Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,650,396	T→C	intergenic (‑89/‑118)	b2520 ← / → b2521	hypothetical protein/putative thiosulfate sulfur transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,650,396	0	T	C	87.2%	624.3 / 72.4	227	intergenic (‑89/‑118)	b2520/b2521	hypothetical protein/putative thiosulfate sulfur transferase
Reads supporting (aligned to +/- strand): ref base T (8/21); new base C (107/91); total (115/112)
Fisher's exact test for biased strand distribution p-value = 9.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 233 total aligned reads displayed.

ATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCC  >  NC_000913/2650306‑2650487

atttttcatCCCTGCCACAATGGTCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCacac                                                                                           >  2:5243047/1‑93 (MQ=255)

atttttcatCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCacac                                                                                           >  2:2027021/1‑93 (MQ=255)

        tCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGaa                                                                                   >  2:4138946/1‑93 (MQ=255)

         cccTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGGGTGAAAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAAt                                                                                  <  1:3907738/93‑1 (MQ=255)

           cTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAAttt                                                                                >  2:146899/1‑93 (MQ=255)

            tGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAAtttt                                                                               >  2:900307/1‑93 (MQ=255)

             gCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTa                                                                              >  1:5182829/1‑93 (MQ=255)

               cacaATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACACCCACACAATCAGAATTTTAGt                                                                            >  2:3433303/1‑93 (MQ=255)

                acaATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTg                                                                           <  1:1485470/93‑1 (MQ=255)

                acaATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTg                                                                           >  2:506032/1‑93 (MQ=255)

                 caATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTga                                                                          <  1:872787/93‑1 (MQ=255)

                  aaTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCATGCCCACACAATCCGAATTTTAGTgag                                                                         >  1:1171712/1‑93 (MQ=255)

                  aaTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTgag                                                                         >  1:1011293/1‑93 (MQ=255)

                  aaTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTgag                                                                         >  1:6043292/1‑93 (MQ=255)

                  aaTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTgag                                                                         <  2:3496729/93‑1 (MQ=255)

                  aaTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTgag                                                                         >  1:3543202/1‑93 (MQ=255)

                  aaTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTgag                                                                         >  2:5913243/1‑93 (MQ=255)

                  aaTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTgag                                                                         >  2:5990679/1‑93 (MQ=255)

                   aTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTgaga                                                                        >  1:301927/1‑93 (MQ=255)

                   aTGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTgaga                                                                        >  1:3360394/1‑93 (MQ=255)

                    tGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGaa                                                                       >  1:5268214/1‑93 (MQ=255)

                     ggCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAAt                                                                      <  1:185886/93‑1 (MQ=255)

                     ggCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAAt                                                                      >  1:1958885/1‑93 (MQ=255)

                     ggCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTAAGTATCACGCCCACACAATCCGAATTTTAGTGAGAAt                                                                      <  2:4007052/93‑1 (MQ=255)

                       cccGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATtg                                                                    >  1:3673268/1‑93 (MQ=255)

                        ccGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATtgt                                                                   <  2:1665973/93‑1 (MQ=255)

                          gTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTgg                                                                 <  1:5256865/93‑1 (MQ=255)

                          gTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTgg                                                                 <  2:2617718/93‑1 (MQ=255)

                          gTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTgg                                                                 <  1:4726843/93‑1 (MQ=255)

                             aGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTc                                                              <  1:4388950/93‑1 (MQ=255)

                              gCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCa                                                             <  1:2785228/93‑1 (MQ=255)

                              gCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCa                                                             <  2:6225338/93‑1 (MQ=255)

                              gCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCa                                                             >  2:1269516/1‑93 (MQ=255)

                                aaCGTTGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACACTCCGAATTTTAGTGAGAATTGTGGCTCAGa                                                           >  1:1369815/1‑93 (MQ=255)

                                aaCGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGa                                                           <  1:2382957/93‑1 (MQ=255)

                                 aCGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGAc                                                          <  2:6545202/93‑1 (MQ=255)

                                    tCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGc                                                       >  2:2203187/1‑93 (MQ=255)

                                    tCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGc                                                       <  1:958136/93‑1 (MQ=255)

                                      gAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCa                                                     >  2:5509031/1‑93 (MQ=255)

                                      gAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCa                                                     >  1:1886672/1‑93 (MQ=255)

                                       aataatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCaa                                                    >  2:4840783/1‑93 (MQ=255)

                                        ataatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCaaa                                                   >  2:5929002/1‑93 (MQ=255)

                                        ataatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCaaa                                                   >  1:1109405/1‑93 (MQ=255)

                                         taatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAAt                                                  >  2:2275496/1‑93 (MQ=255)

                                         taatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAAt                                                  <  1:211384/93‑1 (MQ=255)

                                          aatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATc                                                 <  1:3799064/93‑1 (MQ=255)

                                          aatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATc                                                 <  2:4675426/93‑1 (MQ=255)

                                          aatTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATc                                                 <  2:4436216/93‑1 (MQ=255)

                                            ttattaTGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAA‑TGTGGCTCAGACAGCCAAATCAGc                                              >  2:3750312/1‑93 (MQ=255)

                                              attatGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGcc                                             <  1:3245418/93‑1 (MQ=255)

                                               ttatGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCct                                            >  1:2529529/1‑93 (MQ=255)

                                               ttatGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCct                                            >  1:4352173/1‑93 (MQ=255)

                                               ttatGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCct                                            >  2:502113/1‑93 (MQ=255)

                                                tatGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCctc                                           >  2:5526713/1‑93 (MQ=255)

                                                   gCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCttt                                        <  2:2791744/93‑1 (MQ=255)

                                                   gCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCttt                                        >  1:4033002/1‑93 (MQ=255)

                                                    cGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCtttt                                       <  2:5110234/93‑1 (MQ=255)

                                                    cGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCtttt                                       <  1:915269/93‑1 (MQ=255)

                                                      tGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGc                                     <  2:357353/93‑1 (MQ=255)

                                                       gagaATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCt                                    <  1:4770671/93‑1 (MQ=255)

                                                       gagaATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCt                                    >  2:6059622/1‑93 (MQ=255)

                                                       gagaATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCt                                    >  2:3299516/1‑93 (MQ=255)

                                                       gagaATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCt                                    <  1:160395/93‑1 (MQ=255)

                                                       gagaATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCt                                    <  1:6220946/93‑1 (MQ=255)

                                                        agaATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCtt                                   <  1:2044727/93‑1 (MQ=255)

                                                           aTTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTc                                >  2:5223456/1‑93 (MQ=255)

                                                            tttACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCg                               >  2:3156099/1‑93 (MQ=255)

                                                              tACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCt                             >  1:5774022/1‑93 (MQ=255)

                                                               aCGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCtt                            <  2:1887604/93‑1 (MQ=255)

                                                                cGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCttt                           <  1:3078476/93‑1 (MQ=255)

                                                                 gTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCtttt                          >  2:4560749/1‑93 (MQ=255)

                                                                 gTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCtttt                          >  2:1538962/1‑93 (MQ=255)

                                                                 gTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCtttt                          >  1:3128666/1‑93 (MQ=255)

                                                                  ttATGTAATTCAGTATCACGCCCACACCATCCGAATTTTAGTGAGCATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTc                         >  2:2908845/1‑93 (MQ=255)

                                                                  ttATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTc                         <  1:3632270/93‑1 (MQ=255)

                                                                   tATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCa                        >  2:2240655/1‑93 (MQ=255)

                                                                    aTGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCAc                       <  2:4237661/93‑1 (MQ=255)

                                                                    aTGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCAc                       <  2:2099903/93‑1 (MQ=255)

                                                                     tGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACt                      <  1:4680641/93‑1 (MQ=255)

                                                                     tGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACt                      >  2:5513876/1‑93 (MQ=255)

                                                                        aaTTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTaaa                   >  2:1609901/1‑93 (MQ=255)

                                                                         aTTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAAc                  >  2:1409895/1‑93 (MQ=255)

                                                                          ttCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACa                 >  2:5723231/1‑93 (MQ=255)

                                                                          ttCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACa                 <  1:4104213/93‑1 (MQ=255)

                                                                           tCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACAt                >  2:4635789/1‑93 (MQ=255)

                                                                            cAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATc               <  1:1783521/93‑1 (MQ=255)

                                                                            cAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATc               >  1:6320080/1‑93 (MQ=255)

                                                                             aGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCGGCCTCTTTTGCTTGTCGCTTTTCACTAAACATcc              <  2:3119294/93‑1 (MQ=255)

                                                                             aGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATcc              <  1:4289362/93‑1 (MQ=255)

                                                                             aGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATcc              <  1:5411179/93‑1 (MQ=255)

                                                                                aTCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGAc           >  1:2665034/1‑93 (MQ=255)

                                                                                aTCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGAc           >  2:239002/1‑93 (MQ=255)

                                                                                aTCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGAc           <  2:2647757/93‑1 (MQ=255)

                                                                                aTCACGCACACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGAc           >  1:3693417/1‑93 (MQ=255)

                                                                                    cGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAAtt       >  1:5685670/1‑93 (MQ=255)

                                                                                      cccACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTa     >  2:3932032/1‑93 (MQ=255)

                                                                                       ccACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCTACAATTTaa    >  2:5869521/1‑92 (MQ=255)

                                                                                       ccACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAg    >  2:3956640/1‑93 (MQ=255)

                                                                                       ccACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAg    >  1:4997261/1‑93 (MQ=255)

                                                                                       ccACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAg    <  1:4758573/93‑1 (MQ=255)

ATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCC  >  NC_000913/2650306‑2650487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCTA  >  NC_000913/2650303‑2650489

TTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCAC                                                                                               >  SRR4302547.942662/1‑94 (MQ=60)

TTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCAC                                                                                               >  SRR4302547.886219/1‑94 (MQ=60)

 TCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACA                                                                                              <  SRR4302547.4836020/94‑1 (MQ=60)

   ATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACA                                                                                            <  SRR4302547.5674597/94‑1 (MQ=60)

      TTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATC                                                                                         <  SRR4302547.3300670/94‑1 (MQ=60)

       tccacCCCTGCCACAATGGCCCGTTAGCAACGGCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCCCGCCTACACAATCC                                                                                        <  SRR4302547.6106762/89‑1 (MQ=60)

        TCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCG                                                                                       <  SRR4302547.4036271/94‑1 (MQ=60)

        TCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCG                                                                                       <  SRR4302547.2077534/94‑1 (MQ=60)

         CATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGA                                                                                      >  SRR4302547.4461876/1‑94 (MQ=60)

            CCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGGGTGAAAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATT                                                                                   <  SRR4302547.3987206/94‑1 (MQ=60)

               TGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTA                                                                                >  SRR4302547.3756115/1‑94 (MQ=60)

               TGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTA                                                                                >  SRR4302547.1454727/1‑94 (MQ=60)

               TGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTA                                                                                >  SRR4302547.5289493/1‑94 (MQ=60)

                GCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAG                                                                               >  SRR4302547.1125858/1‑94 (MQ=60)

                GCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAG                                                                               >  SRR4302547.1838628/1‑94 (MQ=60)

                   ACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGA                                                                            <  SRR4302547.3727475/94‑1 (MQ=60)

                   ACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGA                                                                            <  SRR4302547.1515106/94‑1 (MQ=60)

                    CAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCATGCCCACACAATCCGAATTTTAGTGAG                                                                           >  SRR4302547.1195397/1‑94 (MQ=60)

                    CAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAG                                                                           <  SRR4302547.889538/94‑1 (MQ=60)

                    CAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAG                                                                           >  SRR4302547.1031054/1‑94 (MQ=60)

                    CAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAG                                                                           >  SRR4302547.6012583/1‑94 (MQ=60)

                    CAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAG                                                                           >  SRR4302547.3615525/1‑94 (MQ=60)

                    CAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAG                                                                           >  SRR4302547.6168680/1‑94 (MQ=60)

                     AATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGA                                                                          >  SRR4302547.307557/1‑94 (MQ=60)

                     AATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGA                                                                          >  SRR4302547.3428597/1‑94 (MQ=60)

                     AATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGA                                                                          >  SRR4302547.3453240/1‑94 (MQ=60)

                     AATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGA                                                                          >  SRR4302547.4471218/1‑94 (MQ=60)

                      ATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAA                                                                         >  SRR4302547.5376746/1‑94 (MQ=60)

                      ATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAA                                                                         <  SRR4302547.1939333/94‑1 (MQ=60)

                       TGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAAT                                                                        >  SRR4302547.1997771/1‑94 (MQ=60)

                        GGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATT                                                                       <  SRR4302547.2098378/94‑1 (MQ=60)

                        GGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATT                                                                       <  SRR4302547.4808728/94‑1 (MQ=60)

                        GGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATT                                                                       >  SRR4302547.4839950/1‑94 (MQ=60)

                        GGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATT                                                                       <  SRR4302547.189383/94‑1 (MQ=60)

                         GCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTG                                                                      <  SRR4302547.1340245/94‑1 (MQ=60)

                         GCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTG                                                                      >  SRR4302547.2066394/1‑94 (MQ=60)

                         GCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTG                                                                      >  SRR4302547.3748185/1‑94 (MQ=60)

                           CCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTG                                                                    <  SRR4302547.1830000/94‑1 (MQ=60)

                           CCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTG                                                                    >  SRR4302547.4501042/1‑94 (MQ=60)

                            CGTTAGCCACGTCGAATAATTAGTATGCGTGATAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGG                                                                   <  SRR4302547.1621633/94‑1 (MQ=60)

                            CGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGG                                                                   <  SRR4302547.5196450/94‑1 (MQ=60)

                             GTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGC                                                                  <  SRR4302547.5365105/94‑1 (MQ=60)

                             GTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGC                                                                  <  SRR4302547.4824784/94‑1 (MQ=60)

                             GTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGC                                                                  >  SRR4302547.3690019/1‑94 (MQ=60)

                              TTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCT                                                                 >  SRR4302547.3834122/1‑94 (MQ=60)

                               TAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTA                                                                <  SRR4302547.6297527/94‑1 (MQ=60)

                                AGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCA                                                               <  SRR4302547.4479251/94‑1 (MQ=60)

                                 GCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAG                                                              <  SRR4302547.2841016/94‑1 (MQ=60)

                                 GCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAG                                                              >  SRR4302547.5871799/1‑94 (MQ=60)

                                  CAACGTTGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACACTCCGAATTTTAGTGAGAATTGTGGCTCAGA                                                             >  SRR4302547.1397304/1‑94 (MQ=60)

                                   AACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGAC                                                            <  SRR4302547.2431232/94‑1 (MQ=60)

                                   AACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGAC                                                            >  SRR4302547.4144489/1‑94 (MQ=60)

                                   AACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGAC                                                            >  SRR4302547.3078394/1‑94 (MQ=60)

                                      GTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGC                                                         <  SRR4302547.180678/94‑1 (MQ=60)

                                       TCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCC                                                        <  SRR4302547.976745/94‑1 (MQ=60)

                                        CGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCA                                                       <  SRR4302547.6506098/94‑1 (MQ=60)

                                        CGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCA                                                       >  SRR4302547.1923950/1‑94 (MQ=60)

                                          AATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAA                                                     >  SRR4302547.1131743/1‑94 (MQ=60)

                                            TAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATC                                                   <  SRR4302547.215366/94‑1 (MQ=60)

                                             AATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCA                                                  <  SRR4302547.3876512/94‑1 (MQ=60)

                                             AATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCA                                                  <  SRR4302547.2520929/94‑1 (MQ=60)

                                              ATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAG                                                 >  SRR4302547.1436234/1‑94 (MQ=60)

                                                TATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCC                                               >  SRR4302547.2891303/1‑94 (MQ=60)

                                                 ATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCT                                              >  SRR4302547.2580650/1‑94 (MQ=60)

                                                 ATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCT                                              >  SRR4302547.4441597/1‑94 (MQ=60)

                                                 ATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCT                                              <  SRR4302547.3310249/94‑1 (MQ=60)

                                                  TTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTC                                             <  SRR4302547.3293879/94‑1 (MQ=60)

                                                  TTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTC                                             <  SRR4302547.2446824/94‑1 (MQ=60)

                                                   TATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCT                                            <  SRR4302547.1910996/94‑1 (MQ=60)

                                                    ATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTT                                           <  SRR4302547.2309829/94‑1 (MQ=60)

                                                     TGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTT                                          >  SRR4302547.4114897/1‑94 (MQ=60)

                                                      GCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTT                                         <  SRR4302547.5004609/94‑1 (MQ=60)

                                                       CGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTG                                        >  SRR4302547.4397024/1‑94 (MQ=60)

                                                       CGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTG                                        <  SRR4302547.932931/94‑1 (MQ=60)

                                                          GAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTT                                     <  SRR4302547.4869505/94‑1 (MQ=60)

                                                          GAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTT                                     <  SRR4302547.163335/94‑1 (MQ=60)

                                                          GAGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTT                                     <  SRR4302547.6350136/94‑1 (MQ=60)

                                                           AGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTG                                    <  SRR4302547.2085514/94‑1 (MQ=60)

                                                           AGAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTAAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTG                                    <  SRR4302547.5514547/94‑1 (MQ=60)

                                                            GAATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGT                                   <  SRR4302547.4829229/94‑1 (MQ=60)

                                                              ATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCG                                 <  SRR4302547.6442878/94‑1 (MQ=60)

                                                              ATTTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCG                                 >  SRR4302547.6147703/1‑94 (MQ=60)

                                                                TTACGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCT                               >  SRR4302547.5893445/1‑94 (MQ=60)

                                                                   CGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTT                            >  SRR4302547.3190751/1‑94 (MQ=60)

                                                                   CGTTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTT                            <  SRR4302547.3139615/94‑1 (MQ=60)

                                                                     TTATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCA                          <  SRR4302547.3706366/94‑1 (MQ=60)

                                                                      TATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCAC                         <  SRR4302547.5376665/94‑1 (MQ=60)

                                                                       ATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACT                        >  SRR4302547.5336616/1‑94 (MQ=60)

                                                                       ATGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACT                        <  SRR4302547.3394283/94‑1 (MQ=60)

                                                                        TGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTA                       <  SRR4302547.4777595/94‑1 (MQ=60)

                                                                        TGTAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTA                       >  SRR4302547.3814256/1‑94 (MQ=60)

                                                                         GTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAA                      <  SRR4302547.3167697/94‑1 (MQ=60)

                                                                          TAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAA                     >  SRR4302547.3972018/1‑94 (MQ=60)

                                                                          TAATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAA                     >  SRR4302547.2673187/1‑94 (MQ=60)

                                                                            ATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACA                   <  SRR4302547.907219/94‑1 (MQ=60)

                                                                            ATTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACA                   >  SRR4302547.4234780/1‑94 (MQ=60)

                                                                             TTCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACAT                  <  SRR4302547.4187651/94‑1 (MQ=60)

                                                                              TCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATC                 <  SRR4302547.5398222/94‑1 (MQ=60)

                                                                              TCAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATC                 >  SRR4302547.6451492/1‑94 (MQ=60)

                                                                               CAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCC                >  SRR4302547.976376/1‑94 (MQ=60)

                                                                               CAGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCC                <  SRR4302547.1818848/94‑1 (MQ=60)

                                                                                AGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCG               <  SRR4302547.4377519/94‑1 (MQ=60)

                                                                                AGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCG               <  SRR4302547.4857727/94‑1 (MQ=60)

                                                                                AGTATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCG               <  SRR4302547.5523096/94‑1 (MQ=60)

                                                                                 GTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGA              <  SRR4302547.6487349/94‑1 (MQ=60)

                                                                                  TATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCTTCTTTTGCTTGTCGCTTTTCACTAAACATCCGAC             <  SRR4302547.1170610/94‑1 (MQ=60)

                                                                                  TATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGAC             >  SRR4302547.2718586/1‑94 (MQ=60)

                                                                                  TATCACGCACACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGAC             >  SRR4302547.3768820/1‑94 (MQ=60)

                                                                                   ATCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACA            >  SRR4302547.2831008/1‑94 (MQ=60)

                                                                                    TCACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAA           <  SRR4302547.4129310/94‑1 (MQ=60)

                                                                                      ACGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATT         >  SRR4302547.5803073/1‑94 (MQ=60)

                                                                                       CGCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTT        >  SRR4302547.3690099/1‑94 (MQ=60)

                                                                                        GCCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTA       >  SRR4302547.5282250/1‑94 (MQ=60)

                                                                                         CCCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAG      >  SRR4302547.5100593/1‑94 (MQ=60)

                                                                                          CCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGC     >  SRR4302547.175651/1‑94 (MQ=60)

                                                                                          CCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGC     >  SRR4302547.3414537/1‑94 (MQ=60)

                                                                                          CCACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGC     <  SRR4302547.4857167/94‑1 (MQ=60)

                                                                                            ATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCT   <  SRR4302547.3139512/94‑1 (MQ=60)

                                                                                            ACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCT   <  SRR4302547.6393286/94‑1 (MQ=60)

                                                                                            ACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCT   <  SRR4302547.6388791/94‑1 (MQ=60)

                                                                                            ACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCT   <  SRR4302547.3620381/94‑1 (MQ=60)

                                                                                            ACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCT   >  SRR4302547.185313/1‑94 (MQ=60)

                                                                                            ACACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCT   <  SRR4302547.5837796/94‑1 (MQ=60)

                                                                                             CACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCTA  <  SRR4302547.3084822/94‑1 (MQ=60)

TTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCCGAATTTTAGTGAGAATTGTGGCTCAGACAGCCAAATCAGCCTCTTTTGCTTGTCGCTTTTCACTAAACATCCGACAATTTAGCCTA  >  NC_000913/2650303‑2650489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`