Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,660,690 A→G R218R (CGT→CGC)  b2532 ← putative ATP synthase beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,660,6900AG84.8% 614.7 / 93.5 244R218R (CGT→CGC) b2532putative ATP synthase beta subunit
Reads supporting (aligned to +/- strand):  ref base A (21/16);  new base G (108/99);  total (129/115)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 257 total aligned reads displayed.

GCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTCCAGATGAC  >  NC_000913/2660599‑2660781
                                                                                           |                                                                                           
  tGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCgg                                                                                          >  1:1157586/1‑93 (MQ=255)
  tGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCgg                                                                                          >  2:1313410/1‑93 (MQ=255)
  tGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCgg                                                                                          >  1:3599123/1‑93 (MQ=255)
  tGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCgg                                                                                          >  1:4625653/1‑93 (MQ=255)
  tGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCgg                                                                                          >  1:497952/1‑93 (MQ=255)
   gTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCggg                                                                                         >  1:2980162/1‑93 (MQ=255)
     tATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTa                                                                                       >  2:2580583/1‑93 (MQ=255)
        tCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAAc                                                                                    <  1:668723/93‑1 (MQ=255)
            ccTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGAc                                                                                <  1:5429599/93‑1 (MQ=255)
             cTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACg                                                                               <  2:6435670/93‑1 (MQ=255)
             cTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACg                                                                               >  1:4154432/1‑93 (MQ=255)
              ttGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACgg                                                                              >  2:4811938/1‑93 (MQ=255)
              ttGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACgg                                                                              >  1:1359485/1‑93 (MQ=255)
                gTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGgcg                                                                            >  1:1556459/1‑93 (MQ=255)
                gTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGgcg                                                                            <  2:810667/93‑1 (MQ=255)
                  tACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCa                                                                          <  2:3854929/93‑1 (MQ=255)
                    ccTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAAt                                                                        >  2:5909665/1‑93 (MQ=255)
                    ccTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAAt                                                                        >  2:3578268/1‑93 (MQ=255)
                     cTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAAtt                                                                       >  1:5444947/1‑93 (MQ=255)
                     cTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAAtt                                                                       >  1:4572911/1‑93 (MQ=255)
                     cTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAAtt                                                                       >  2:1108667/1‑93 (MQ=255)
                      tttATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAAttt                                                                      >  2:6603600/1‑93 (MQ=255)
                       ttATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTa                                                                     >  1:4825140/1‑93 (MQ=255)
                         aTTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTAtt                                                                   >  2:2748191/1‑93 (MQ=255)
                           tCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATtca                                                                 <  1:6370185/93‑1 (MQ=255)
                            ctgctgCTCAATAGAAGCCACAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATtcat                                                                >  1:878354/1‑93 (MQ=255)
                             tgctgcTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATtaatc                                                               <  1:5154858/93‑1 (MQ=255)
                               ctgctCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCAc                                                             <  2:1394278/93‑1 (MQ=255)
                               ctgctCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCAc                                                             <  1:3416303/93‑1 (MQ=255)
                                tgctCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCAcc                                                            >  2:954915/1‑93 (MQ=255)
                                tgctCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCAcc                                                            <  2:5515276/93‑1 (MQ=255)
                                cgctCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCAcc                                                            <  2:1859801/92‑1 (MQ=255)
                                 gctCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCt                                                           <  1:6587659/93‑1 (MQ=255)
                                 gctCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCt                                                           <  1:4876923/93‑1 (MQ=255)
                                    cAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGcc                                                        <  2:2034782/93‑1 (MQ=255)
                                    cAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGcc                                                        <  1:5579828/93‑1 (MQ=255)
                                      aTAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGCCGGCGCAATTTATTCATCACCTGcccc                                                      >  1:2148086/1‑93 (MQ=255)
                                      aTAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGcccc                                                      >  2:5680743/1‑93 (MQ=255)
                                       tAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCg                                                     >  1:5836246/1‑93 (MQ=255)
                                        aGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCgg                                                    <  1:112704/93‑1 (MQ=255)
                                        aGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCgg                                                    <  1:4088519/93‑1 (MQ=255)
                                         gAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGa                                                   <  1:1898178/93‑1 (MQ=255)
                                          aaGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGAt                                                  <  2:1515990/93‑1 (MQ=255)
                                           aGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATg                                                 >  2:3225556/1‑93 (MQ=255)
                                           aGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATg                                                 >  2:4344567/1‑93 (MQ=255)
                                            gCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATgg                                                <  1:388759/93‑1 (MQ=255)
                                              cAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGtt                                              <  1:3569139/93‑1 (MQ=255)
                                               aGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGttt                                             >  1:6119338/1‑93 (MQ=255)
                                                 aaTCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTc                                           <  1:902142/93‑1 (MQ=255)
                                                 aaTCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTc                                           >  1:4296563/1‑93 (MQ=255)
                                                  aTCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCa                                          >  1:5043228/1‑93 (MQ=255)
                                                  aTCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCa                                          <  1:2843049/93‑1 (MQ=255)
                                                  aTCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCa                                          >  2:3122828/1‑93 (MQ=255)
                                                   tCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAc                                         <  1:3084233/93‑1 (MQ=255)
                                                   tCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAc                                         >  2:3802864/1‑93 (MQ=255)
                                                    cccGCGCAGGATATTCAACTCCTGGCTTTCCGCGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTCCCCCGGATGGCTTTCatg                                        >  1:384986/1‑91 (MQ=255)
                                                     ccGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACgg                                       <  2:4279328/93‑1 (MQ=255)
                                                     ccGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACgg                                       >  2:3022994/1‑93 (MQ=255)
                                                      cgcgCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGa                                      <  2:1748736/93‑1 (MQ=255)
                                                         gcAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATaa                                   <  2:3651865/93‑1 (MQ=255)
                                                           aGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATaaaa                                 >  1:6633721/1‑93 (MQ=255)
                                                            ggATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAAc                                >  2:4591157/1‑93 (MQ=255)
                                                             gATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAAcc                               >  2:4922480/1‑93 (MQ=255)
                                                              atatTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCg                              <  2:2846326/93‑1 (MQ=255)
                                                               tatTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCgg                             >  2:3213636/1‑93 (MQ=255)
                                                                atTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGt                            <  1:3755657/93‑1 (MQ=255)
                                                                 ttCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGtt                           >  2:3396619/1‑93 (MQ=255)
                                                                  tCAACACCTGGCTTTCCGGGCGCGCGCGGATAAACAGACGGAGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTg                          <  2:6643723/93‑1 (MQ=255)
                                                                   cAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGc                         >  2:1687406/1‑93 (MQ=255)
                                                                    agcTCCTGGTTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGcc                        <  1:1497849/91‑1 (MQ=255)
                                                                    aaCTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGcc                        >  1:4913075/1‑93 (MQ=255)
                                                                    aaCTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGcc                        <  1:981682/93‑1 (MQ=255)
                                                                    aaCTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGcc                        >  1:3128977/1‑93 (MQ=255)
                                                                     aCTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCca                       >  2:2344225/1‑93 (MQ=255)
                                                                     aCTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCca                       >  2:4463118/1‑93 (MQ=255)
                                                                     aCTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCca                       >  1:2219244/1‑93 (MQ=255)
                                                                      cTCCTGGATTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCcag                      <  1:397742/93‑1 (MQ=255)
                                                                        ccTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCctgca                    <  2:5085060/93‑1 (MQ=255)
                                                                        ccTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCcagca                    <  1:33438/93‑1 (MQ=255)
                                                                         cttgCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGTAATTTATTCATCTCCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCcagcag                   <  1:5866939/90‑1 (MQ=255)
                                                                         cTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCcagcag                   >  1:6089042/1‑93 (MQ=255)
                                                                         cTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCcagcag                   <  1:560033/93‑1 (MQ=255)
                                                                         cTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCcagcag                   >  2:2060627/1‑93 (MQ=255)
                                                                         cTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCcagcag                   >  1:1048748/1‑93 (MQ=255)
                                                                          tGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCagcagc                  >  1:570760/1‑93 (MQ=255)
                                                                          tGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCagcagc                  >  2:4969770/1‑93 (MQ=255)
                                                                           ggCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCg                 >  1:1245529/1‑93 (MQ=255)
                                                                            gcgttCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGt                <  1:1669121/90‑1 (MQ=255)
                                                                            gCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGt                <  1:6241646/93‑1 (MQ=255)
                                                                              tttCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGttt              >  1:5247809/1‑93 (MQ=255)
                                                                                 ccGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGtt           <  2:2263700/93‑1 (MQ=255)
                                                                                  cGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTc          >  2:6260265/1‑93 (MQ=255)
                                                                                  cGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTc          <  1:4044897/93‑1 (MQ=255)
                                                                                   gggCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTcc         >  1:2896758/1‑93 (MQ=255)
                                                                                   gggCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTcc         >  2:2169724/1‑93 (MQ=255)
                                                                                   gggCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTcc         <  1:6521678/93‑1 (MQ=255)
                                                                                       gcgcgcGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTCCAGAt     <  1:2766224/93‑1 (MQ=255)
                                                                                       gcgcgcGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTCCAGAt     <  2:5307174/93‑1 (MQ=255)
                                                                                        cgcgcgGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTCCAGATg    <  1:2559258/93‑1 (MQ=255)
                                                                                          cACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTCGAGATGAc  >  2:867680/1‑93 (MQ=255)
                                                                                           |                                                                                           
GCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGTTTGTTCCAGATGAC  >  NC_000913/2660599‑2660781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATGACC  >  NC_000913/2660597‑2660782
                                                                                             |                                                                                             
CGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCG                                                                                               >  SRR4302547.4421753/1‑94 (MQ=60)
 GGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCAC                                                                                              >  SRR4302547.5210412/1‑94 (MQ=60)
  GCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCG                                                                                             <  SRR4302547.6603050/94‑1 (MQ=60)
   CTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGG                                                                                            >  SRR4302547.1181005/1‑94 (MQ=60)
   CTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGG                                                                                            >  SRR4302547.4721456/1‑94 (MQ=60)
   CTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGG                                                                                            >  SRR4302547.3672510/1‑94 (MQ=60)
   CTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGG                                                                                            >  SRR4302547.507028/1‑94 (MQ=60)
    TGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGG                                                                                           >  SRR4302547.3039463/1‑94 (MQ=60)
    TGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGG                                                                                           >  SRR4302547.5000059/1‑94 (MQ=60)
    TGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGG                                                                                           <  SRR4302547.426668/94‑1 (MQ=60)
     GTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGT                                                                                          >  SRR4302547.6553167/1‑94 (MQ=60)
     GTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGT                                                                                          <  SRR4302547.2920828/94‑1 (MQ=60)
      TTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTA                                                                                         >  SRR4302547.6714275/1‑94 (MQ=60)
      TTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTA                                                                                         >  SRR4302547.6019205/1‑94 (MQ=60)
       TATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAAGTCCTGGCTTTCCGGGCGCTCGCGGGTAA                                                                                        >  SRR4302547.6015498/1‑94 (MQ=60)
          TCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACA                                                                                     <  SRR4302547.681030/94‑1 (MQ=60)
          TCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACA                                                                                     <  SRR4302547.3368937/94‑1 (MQ=60)
              CCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACG                                                                                 <  SRR4302547.4977282/94‑1 (MQ=60)
              CCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACG                                                                                 <  SRR4302547.6324694/94‑1 (MQ=60)
              CCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACG                                                                                 <  SRR4302547.5541865/94‑1 (MQ=60)
              CCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACG                                                                                 >  SRR4302547.4239061/1‑94 (MQ=60)
              CCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACG                                                                                 >  SRR4302547.2436326/1‑94 (MQ=60)
              CCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACG                                                                                 <  SRR4302547.1984494/94‑1 (MQ=60)
              CCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACG                                                                                 >  SRR4302547.5754944/1‑94 (MQ=60)
               CTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGG                                                                                >  SRR4302547.4064746/1‑94 (MQ=60)
               CTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGG                                                                                <  SRR4302547.6220698/94‑1 (MQ=60)
               CTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGG                                                                                >  SRR4302547.1386765/1‑94 (MQ=60)
                TTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGC                                                                               <  SRR4302547.4988007/94‑1 (MQ=60)
                TTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGC                                                                               >  SRR4302547.6793042/1‑94 (MQ=60)
                 TGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCG                                                                              >  SRR4302547.1587425/1‑94 (MQ=60)
                 TGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCG                                                                              <  SRR4302547.1587137/94‑1 (MQ=60)
                   TTACCTTTCTTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCA                                                                            >  SRR4302547.6675498/1‑94 (MQ=60)
                    TACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAA                                                                           >  SRR4302547.3953581/1‑94 (MQ=60)
                    TACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAA                                                                           <  SRR4302547.1528366/94‑1 (MQ=60)
                    TACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGAAGGCGCAA                                                                           >  SRR4302547.899841/1‑94 (MQ=60)
                      CCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATT                                                                         >  SRR4302547.4667535/1‑94 (MQ=60)
                      CCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATT                                                                         >  SRR4302547.5557450/1‑94 (MQ=60)
                      CCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATT                                                                         >  SRR4302547.1371292/1‑94 (MQ=60)
                      CCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATT                                                                         <  SRR4302547.4719823/94‑1 (MQ=60)
                       CTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGGGCGCGCGGGCAAACAGACGGCGCAATTT                                                                        >  SRR4302547.4788316/1‑94 (MQ=60)
                        TTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTA                                                                       >  SRR4302547.4925177/1‑94 (MQ=60)
                         TTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTAT                                                                      <  SRR4302547.4138122/94‑1 (MQ=60)
                         TTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTAT                                                                      <  SRR4302547.5709315/94‑1 (MQ=60)
                          TATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATT                                                                     <  SRR4302547.5358320/94‑1 (MQ=60)
                           ATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTC                                                                    >  SRR4302547.4203352/1‑94 (MQ=60)
                            TTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCA                                                                   <  SRR4302547.1767415/94‑1 (MQ=60)
                             TCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCAT                                                                  <  SRR4302547.3988808/94‑1 (MQ=60)
                             TCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCAT                                                                  <  SRR4302547.6503095/94‑1 (MQ=60)
                             TCTGCTGCTCAATAGAAGCCACAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCAT                                                                  >  SRR4302547.895231/1‑94 (MQ=60)
                              CTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATC                                                                 >  SRR4302547.37359/1‑94 (MQ=60)
                              CTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATC                                                                 >  SRR4302547.5137704/1‑94 (MQ=60)
                               TGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCA                                                                >  SRR4302547.5729567/1‑94 (MQ=60)
                               TGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTAATCA                                                                <  SRR4302547.5261013/94‑1 (MQ=60)
                                 CTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACC                                                              <  SRR4302547.3485871/94‑1 (MQ=60)
                                 CTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACC                                                              <  SRR4302547.4786406/94‑1 (MQ=60)
                                 CTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACC                                                              >  SRR4302547.3454097/1‑94 (MQ=60)
                                  TGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCT                                                             <  SRR4302547.5973206/94‑1 (MQ=60)
                                   GCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTG                                                            <  SRR4302547.4977966/94‑1 (MQ=60)
                                   GCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTG                                                            <  SRR4302547.6724806/94‑1 (MQ=60)
                                    CTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGC                                                           >  SRR4302547.1274529/1‑94 (MQ=60)
                                      CAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCC                                                         >  SRR4302547.6738431/1‑94 (MQ=60)
                                      CAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCC                                                         <  SRR4302547.5694894/94‑1 (MQ=60)
                                      CAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCC                                                         >  SRR4302547.3686443/1‑94 (MQ=60)
                                       AATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGCCGGCGCAATTTATTCATCACCTGCCCC                                                        >  SRR4302547.2190953/1‑94 (MQ=60)
                                        ATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCG                                                       >  SRR4302547.5956997/1‑94 (MQ=60)
                                          AGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGA                                                     <  SRR4302547.114756/94‑1 (MQ=60)
                                          AGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGA                                                     <  SRR4302547.4171650/94‑1 (MQ=60)
                                           GAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGAT                                                    <  SRR4302547.1935732/94‑1 (MQ=60)
                                           GAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGAT                                                    >  SRR4302547.1517037/1‑94 (MQ=60)
                                           GAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGAT                                                    <  SRR4302547.1935733/94‑1 (MQ=60)
                                           GAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGAT                                                    >  SRR4302547.1163969/1‑94 (MQ=60)
                                            AAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATG                                                   <  SRR4302547.584369/94‑1 (MQ=60)
                                             AGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGG                                                  <  SRR4302547.647064/94‑1 (MQ=60)
                                              GCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGT                                                 <  SRR4302547.395968/94‑1 (MQ=60)
                                              GCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGT                                                 >  SRR4302547.3567553/1‑94 (MQ=60)
                                               CCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTT                                                >  SRR4302547.3655460/1‑94 (MQ=60)
                                                CAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTT                                               <  SRR4302547.3641948/94‑1 (MQ=60)
                                                CAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTT                                               >  SRR4302547.6246265/1‑94 (MQ=60)
                                                 AGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTT                                              >  SRR4302547.3259187/1‑94 (MQ=60)
                                                  GAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTC                                             >  SRR4302547.4384901/1‑94 (MQ=60)
                                                   AATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCA                                            >  SRR4302547.5147368/1‑94 (MQ=60)
                                                   AATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCA                                            <  SRR4302547.919506/94‑1 (MQ=60)
                                                   AATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCA                                            >  SRR4302547.288345/1‑94 (MQ=60)
                                                   AATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCA                                            >  SRR4302547.2841934/1‑94 (MQ=60)
                                                    ATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAC                                           <  SRR4302547.4290550/94‑1 (MQ=60)
                                                    ATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAC                                           <  SRR4302547.2899860/94‑1 (MQ=60)
                                                    ATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAC                                           <  SRR4302547.6788273/94‑1 (MQ=60)
                                                     TCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACG                                          <  SRR4302547.3145452/94‑1 (MQ=60)
                                                     TCCCGCGCAGGATATTCAACTCCTGGCTTTCCGCGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTCCCCCGGATGGCTTTCATG                                          >  SRR4302547.392123/1‑94 (MQ=60)
                                                        CGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGAT                                       <  SRR4302547.907149/94‑1 (MQ=60)
                                                           GCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAA                                    >  SRR4302547.1133614/1‑94 (MQ=60)
                                                           GCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAA                                    >  SRR4302547.5060144/1‑94 (MQ=60)
                                                           GCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTACCCCCGATGGTTTTCACGGATAAA                                    >  SRR4302547.1911544/1‑94 (MQ=60)
                                                            CAGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAA                                   >  SRR4302547.6771803/1‑94 (MQ=60)
                                                             AGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAAC                                  <  SRR4302547.5175468/94‑1 (MQ=60)
                                                             AGGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAAC                                  >  SRR4302547.5302953/1‑94 (MQ=60)
                                                              GGATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACC                                 <  SRR4302547.6642408/94‑1 (MQ=60)
                                                                ATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGG                               <  SRR4302547.995628/94‑1 (MQ=60)
                                                                ATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGG                               >  SRR4302547.5548774/1‑94 (MQ=60)
                                                                ATATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGG                               >  SRR4302547.3159008/1‑94 (MQ=60)
                                                                  ATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTT                             <  SRR4302547.3525061/94‑1 (MQ=60)
                                                                  ATTCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTT                             <  SRR4302547.3832283/94‑1 (MQ=60)
                                                                    TCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGC                           >  SRR4302547.1203418/1‑94 (MQ=60)
                                                                    TCAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGC                           <  SRR4302547.858867/94‑1 (MQ=60)
                                                                     CAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCC                          >  SRR4302547.5014810/1‑94 (MQ=60)
                                                                     CAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCC                          >  SRR4302547.445308/1‑94 (MQ=60)
                                                                     CAACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCC                          >  SRR4302547.3191068/1‑94 (MQ=60)
                                                                      AGCTCCTGGTTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCA                         <  SRR4302547.1527734/94‑1 (MQ=60)
                                                                      AGCTCCTGGGTTGCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCA                         <  SRR4302547.2672440/94‑1 (MQ=60)
                                                                      AACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCA                         <  SRR4302547.1000782/94‑1 (MQ=60)
                                                                      AACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCA                         >  SRR4302547.2263948/1‑94 (MQ=60)
                                                                       ACTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAG                        >  SRR4302547.5674096/1‑94 (MQ=60)
                                                                       ACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGCCGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAG                        >  SRR4302547.5508740/1‑94 (MQ=60)
                                                                        CTCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGC                       >  SRR4302547.1695533/1‑94 (MQ=60)
                                                                        CTCCTGGATTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGC                       <  SRR4302547.405141/94‑1 (MQ=60)
                                                                         TCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCA                      >  SRR4302547.5870592/1‑94 (MQ=60)
                                                                         TCCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCA                      <  SRR4302547.412051/94‑1 (MQ=60)
                                                                          CCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAG                     <  SRR4302547.34046/94‑1 (MQ=60)
                                                                          CCTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAG                     >  SRR4302547.6215351/1‑94 (MQ=60)
                                                                          CCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAG                     >  SRR4302547.1069506/1‑94 (MQ=60)
                                                                          CCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAG                     >  SRR4302547.3010983/1‑94 (MQ=60)
                                                                           CTTGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGTAATTTATTCATCTCCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGC                    <  SRR4302547.5988367/94‑1 (MQ=60)
                                                                           CTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGC                    <  SRR4302547.570117/94‑1 (MQ=60)
                                                                           CTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGC                    <  SRR4302547.2960484/94‑1 (MQ=60)
                                                                           CTGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGC                    >  SRR4302547.581019/1‑94 (MQ=60)
                                                                           CTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGC                    <  SRR4302547.2335212/94‑1 (MQ=60)
                                                                            TGGCTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG                   >  SRR4302547.1270668/1‑94 (MQ=60)
                                                                            TGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGCATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG                   >  SRR4302547.2222262/1‑94 (MQ=60)
                                                                              GCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TT                <  SRR4302547.3885337/94‑1 (MQ=60)
                                                                              GCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TT                <  SRR4302547.6371310/94‑1 (MQ=60)
                                                                              GCGTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TT                <  SRR4302547.1702014/94‑1 (MQ=60)
                                                                               CTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTT               <  SRR4302547.3070565/94‑1 (MQ=60)
                                                                               CTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTT               >  SRR4302547.5355840/1‑94 (MQ=60)
                                                                               CTTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTT               >  SRR4302547.444192/1‑94 (MQ=60)
                                                                               CTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTT               <  SRR4302547.1167236/94‑1 (MQ=60)
                                                                                TTTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCGATTT               >  SRR4302547.5609838/1‑94 (MQ=60)
                                                                                 TTCCGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGT             >  SRR4302547.4053976/1‑94 (MQ=60)
                                                                                    CGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCC          >  SRR4302547.2954526/1‑94 (MQ=60)
                                                                                    CGGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCC          <  SRR4302547.4127033/94‑1 (MQ=60)
                                                                                     GGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCA         <  SRR4302547.6657446/94‑1 (MQ=60)
                                                                                     GGGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCA         <  SRR4302547.3073313/94‑1 (MQ=60)
                                                                                      GGCGCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAG        <  SRR4302547.5127788/94‑1 (MQ=60)
                                                                                         GCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATG     <  SRR4302547.2821652/94‑1 (MQ=60)
                                                                                         GCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATG     <  SRR4302547.3896273/94‑1 (MQ=60)
                                                                                         GCGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATG     <  SRR4302547.6297354/94‑1 (MQ=60)
                                                                                          CGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATGA    <  SRR4302547.2610943/94‑1 (MQ=60)
                                                                                          CGCGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATGA    >  SRR4302547.2180544/1‑94 (MQ=60)
                                                                                            CGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATGACC  <  SRR4302547.2198399/94‑1 (MQ=60)
                                                                                            CGCGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAAGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATGACC  >  SRR4302547.4496582/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAACCGGTTGCCAGCAGCG‑TTTGTTCCAGATGACC  >  NC_000913/2660597‑2660782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑