Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,748,385	A→G	D115D (GAT→GAC)	grpE ←	GrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,748,385	0	A	G	99.6%	938.8 / ‑4.6	252	D115D (GAT→GAC)	grpE	GrpE
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (146/105); total (147/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 255 total aligned reads displayed.

AGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATATCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAA  >  NC_000913/2748295‑2748474

aGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTc                                                                                         <  2:5365271/93‑1 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        >  1:2331001/1‑93 (MQ=255)

 gTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTcc                                                                                        >  2:1822419/1‑93 (MQ=255)

  tttCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCg                                                                                       <  1:1255699/93‑1 (MQ=255)

  tttCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCg                                                                                       <  2:4137518/93‑1 (MQ=255)

    tCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCggg                                                                                     >  2:1069559/1‑93 (MQ=255)

     cGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGt                                                                                    >  1:2178847/1‑93 (MQ=255)

       gCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTa                                                                                  <  1:1762052/93‑1 (MQ=255)

       gCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTa                                                                                  <  1:2036195/93‑1 (MQ=255)

        cGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAg                                                                                 <  1:290490/93‑1 (MQ=255)

         gATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGc                                                                                >  1:4261587/1‑93 (MQ=255)

         gATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGc                                                                                <  1:4964411/93‑1 (MQ=255)

         gATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGc                                                                                <  2:6370016/93‑1 (MQ=255)

           tCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCtt                                                                              >  2:6595355/1‑93 (MQ=255)

            cACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCttt                                                                             <  2:5491678/93‑1 (MQ=255)

            cACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCttt                                                                             >  2:4018016/1‑93 (MQ=255)

             aCTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTa                                                                            <  1:2853777/93‑1 (MQ=255)

              cTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAt                                                                           <  2:2212565/93‑1 (MQ=255)

               ttCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATc                                                                          <  2:2364243/93‑1 (MQ=255)

                  aaCGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGc                                                                       <  1:692227/93‑1 (MQ=255)

                   aCGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGcc                                                                      >  2:6220388/1‑93 (MQ=255)

                   aCGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGcc                                                                      <  2:6139468/93‑1 (MQ=255)

                   aCGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGcc                                                                      >  1:5239093/1‑93 (MQ=255)

                    cGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCa                                                                     <  1:2510780/93‑1 (MQ=255)

                    cGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCa                                                                     <  2:342843/93‑1 (MQ=255)

                     gCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCAc                                                                    >  2:839805/1‑93 (MQ=255)

                      ccAAACTTACGCACACCATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACt                                                                   <  2:44018/93‑1 (MQ=255)

                      ccAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACt                                                                   <  2:4449753/93‑1 (MQ=255)

                      ccAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACt                                                                   <  1:991836/93‑1 (MQ=255)

                        aaaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTc                                                                 >  1:2125257/1‑93 (MQ=255)

                         aaCTTACGCACAACATCCAGCATCGACTTCAGGGGCAGCTCAATGCCTTCAACCATCGCAGACAGGTCCGGGTTAGCTTTATCAGCCACTTcc                                                                <  1:418906/93‑1 (MQ=255)

                         aaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTcc                                                                >  2:2716574/1‑93 (MQ=255)

                         aaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTcc                                                                >  1:2629937/1‑93 (MQ=255)

                         aaCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTcc                                                                <  1:5434680/93‑1 (MQ=255)

                          aCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCa                                                               <  2:3946942/93‑1 (MQ=255)

                          aCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCa                                                               <  1:789306/93‑1 (MQ=255)

                          aCTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCa                                                               >  1:503604/1‑93 (MQ=255)

                           cTTACGCACAACGTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGCTAGCTTTATCAGCCACTTCCAg                                                              >  1:5657131/1‑93 (MQ=255)

                           cTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAg                                                              <  1:877269/93‑1 (MQ=255)

                           cTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAg                                                              >  2:2140072/1‑93 (MQ=255)

                            ttACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAgc                                                             <  1:4882722/93‑1 (MQ=255)

                            ttACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAgc                                                             >  2:3676841/1‑93 (MQ=255)

                                gCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCAc                                                         >  2:5093256/1‑93 (MQ=255)

                                 cacaACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACg                                                        <  1:1324393/93‑1 (MQ=255)

                                  acaacaTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGa                                                       >  2:6434037/1‑93 (MQ=255)

                                  acaacaTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGa                                                       <  2:1591343/93‑1 (MQ=255)

                                  acaacaTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGa                                                       <  1:1545878/93‑1 (MQ=255)

                                   caacaTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGAt                                                      <  2:6490075/93‑1 (MQ=255)

                                    aacaTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATc                                                     >  1:6529922/1‑93 (MQ=255)

                                       aTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAg                                                  <  2:6100745/93‑1 (MQ=255)

                                         ccAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGc                                                <  2:958035/93‑1 (MQ=255)

                                           agaaTCAACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCTCACGATCCAGGCTa                                              <  2:1224574/90‑1 (MQ=255)

                                           aGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTa                                              >  1:2572866/1‑93 (MQ=255)

                                           aGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTa                                              >  2:4607074/1‑93 (MQ=255)

                                              aTCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatca                                           <  1:6508007/93‑1 (MQ=255)

                                              aTCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatca                                           <  1:138913/93‑1 (MQ=255)

                                              aTCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatca                                           >  2:2436633/1‑93 (MQ=255)

                                               tCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaa                                          >  1:4411161/1‑93 (MQ=255)

                                               tCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaa                                          >  1:1871491/1‑93 (MQ=255)

                                               tCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaa                                          >  1:4467997/1‑93 (MQ=255)

                                               tCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaa                                          >  1:57346/1‑93 (MQ=255)

                                                cGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaat                                         <  2:1139340/93‑1 (MQ=255)

                                                cGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaat                                         <  1:4858578/93‑1 (MQ=255)

                                                 gACTTCAGCGACAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaatc                                        <  1:3756812/93‑1 (MQ=255)

                                                 gACTTCAACGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCATGATCCAGGCTatcaatc                                        >  1:6311726/1‑93 (MQ=255)

                                                  aCTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGCCATGTCCGGGTTAGCTTTATCAGCCCCTTCCACCGCACGATCCAGCCTatcaatac                                       >  2:716445/1‑91 (MQ=255)

                                                  aCTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTatcaatca                                       >  2:3216657/1‑93 (MQ=255)

                                                   cTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAc                                      <  2:5357335/93‑1 (MQ=255)

                                                   cTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAc                                      >  1:3533332/1‑93 (MQ=255)

                                                         cGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGgcag                                >  2:3814563/1‑93 (MQ=255)

                                                         cGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGGTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGTCTATCACGCACCGgcgt                                >  1:6452102/1‑91 (MQ=255)

                                                             aGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAt                            >  2:4091655/1‑93 (MQ=255)

                                                              gCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAtt                           >  2:6049182/1‑93 (MQ=255)

                                                              gCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAtt                           >  2:5068898/1‑93 (MQ=255)

                                                                tCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCg                         >  1:2090598/1‑93 (MQ=255)

                                                                 cAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGt                        <  2:4192415/93‑1 (MQ=255)

                                                                 cAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGt                        <  2:5172930/93‑1 (MQ=255)

                                                                  aaTGCCTTCAACCATCGCAGACATGTCCGGGTTCGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGtt                       <  1:6104094/93‑1 (MQ=255)

                                                                  aaTGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGtt                       >  2:2538856/1‑93 (MQ=255)

                                                                   aTGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTtg                      >  1:1484486/1‑93 (MQ=255)

                                                                    tGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTtga                     <  1:4169025/93‑1 (MQ=255)

                                                                    tGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTtga                     <  2:3115417/93‑1 (MQ=255)

                                                                     gCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTtgat                    >  1:838484/1‑93 (MQ=255)

                                                                       cTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTtgatga                  >  1:6027349/1‑93 (MQ=255)

                                                                        ttCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGaa                 >  1:3393311/1‑93 (MQ=255)

                                                                          cAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAAtt               >  1:270816/1‑93 (MQ=255)

                                                                           aaCCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAAttt              >  1:4949136/1‑93 (MQ=255)

                                                                           aaCCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAAttt              >  1:1132892/1‑93 (MQ=255)

                                                                           aaCCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAAttt              <  1:2240924/93‑1 (MQ=255)

                                                                            aCCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTtc             >  1:5569092/1‑93 (MQ=255)

                                                                              cATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTtctc           <  2:3395020/93‑1 (MQ=255)

                                                                               aTCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTcc          <  1:292844/93‑1 (MQ=255)

                                                                               aTCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTcc          <  2:4950441/93‑1 (MQ=255)

                                                                                tCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCa         <  2:1346412/93‑1 (MQ=255)

                                                                                  gCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAgc       <  1:5002448/93‑1 (MQ=255)

                                                                                   cAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAgcg      >  1:2354119/1‑93 (MQ=255)

                                                                                   cAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAgcg      <  1:4750406/93‑1 (MQ=255)

                                                                                    aGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAgcgc     <  2:4825948/93‑1 (MQ=255)

                                                                                    aGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAgcgc     <  2:2409506/93‑1 (MQ=255)

                                                                                     gACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGcgcg    >  2:5936642/1‑93 (MQ=255)

AGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATATCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAA  >  NC_000913/2748295‑2748474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATATCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTG  >  NC_000913/2748292‑2748478

GTTAGTTTCGGAGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATG                                                                                               >  SRR4302547.4144350/1‑94 (MQ=60)

 TTAGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGT                                                                                              <  SRR4302547.111506/94‑1 (MQ=60)

 TTAGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGT                                                                                              <  SRR4302547.1426785/94‑1 (MQ=60)

 TTAGTTTCGGCGATCAATTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGT                                                                                              >  SRR4302547.6007232/1‑94 (MQ=60)

   AGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCC                                                                                            >  SRR4302547.2815718/1‑94 (MQ=60)

   AGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCC                                                                                            >  SRR4302547.2378254/1‑94 (MQ=60)

    GTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCG                                                                                           <  SRR4302547.1355756/94‑1 (MQ=60)

     TTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGG                                                                                          <  SRR4302547.1281016/94‑1 (MQ=60)

       TCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGT                                                                                        >  SRR4302547.5014740/1‑94 (MQ=60)

       TCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGT                                                                                        >  SRR4302547.2222475/1‑94 (MQ=60)

        CGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTT                                                                                       <  SRR4302547.4360311/94‑1 (MQ=60)

        CGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTT                                                                                       >  SRR4302547.2660610/1‑94 (MQ=60)

         GGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTA                                                                                      <  SRR4302547.6719038/94‑1 (MQ=60)

          GCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAG                                                                                     <  SRR4302547.1350355/94‑1 (MQ=60)

          GCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAG                                                                                     <  SRR4302547.2076779/94‑1 (MQ=60)

          GCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAG                                                                                     <  SRR4302547.237745/94‑1 (MQ=60)

          GCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAG                                                                                     <  SRR4302547.2449034/94‑1 (MQ=60)

          GCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAG                                                                                     <  SRR4302547.1796998/94‑1 (MQ=60)

           CGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGC                                                                                    >  SRR4302547.1192867/1‑94 (MQ=60)

           CGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGC                                                                                    <  SRR4302547.295880/94‑1 (MQ=60)

           CGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGC                                                                                    >  SRR4302547.5278034/1‑94 (MQ=60)

           CGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGC                                                                                    >  SRR4302547.4348800/1‑94 (MQ=60)

            GATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCT                                                                                   >  SRR4302547.3612668/1‑94 (MQ=60)

            GATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCT                                                                                   >  SRR4302547.438260/1‑94 (MQ=60)

            GATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCT                                                                                   <  SRR4302547.5067144/94‑1 (MQ=60)

             ATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTT                                                                                  >  SRR4302547.1608409/1‑94 (MQ=60)

             ATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTT                                                                                  <  SRR4302547.6639468/94‑1 (MQ=60)

              TCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTT                                                                                 >  SRR4302547.1905539/1‑94 (MQ=60)

               CACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTA                                                                                >  SRR4302547.2273057/1‑94 (MQ=60)

                ACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAT                                                                               <  SRR4302547.2910802/94‑1 (MQ=60)

                ACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAT                                                                               <  SRR4302547.2933896/94‑1 (MQ=60)

                ACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTAT                                                                               >  SRR4302547.6491012/1‑94 (MQ=60)

                  TTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCA                                                                             >  SRR4302547.297113/1‑94 (MQ=60)

                  TTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCA                                                                             >  SRR4302547.5590790/1‑94 (MQ=60)

                    CAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGC                                                                           >  SRR4302547.2598100/1‑94 (MQ=60)

                     AACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCC                                                                          >  SRR4302547.5346942/1‑94 (MQ=60)

                     AACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCC                                                                          <  SRR4302547.705012/94‑1 (MQ=60)

                      ACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCA                                                                         <  SRR4302547.807909/94‑1 (MQ=60)

                      ACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCA                                                                         >  SRR4302547.5071763/1‑94 (MQ=60)

                       CGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCAC                                                                        <  SRR4302547.2561528/94‑1 (MQ=60)

                       CGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCAC                                                                        >  SRR4302547.4257794/1‑94 (MQ=60)

                         CCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTT                                                                      <  SRR4302547.1011152/94‑1 (MQ=60)

                         CCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTT                                                                      >  SRR4302547.4996055/1‑94 (MQ=60)

                         CCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTT                                                                      <  SRR4302547.2991964/94‑1 (MQ=60)

                          CAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTC                                                                     >  SRR4302547.2167608/1‑94 (MQ=60)

                          CAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTC                                                                     <  SRR4302547.4738837/94‑1 (MQ=60)

                          CAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTC                                                                     >  SRR4302547.754013/1‑94 (MQ=60)

                           AAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCC                                                                    <  SRR4302547.5690077/94‑1 (MQ=60)

                           AAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCC                                                                    >  SRR4302547.2682908/1‑94 (MQ=60)

                            AACTTACGCACAACATCCAGCATCGACTTCAGGGGCAGCTCAATGCCTTCAACCATCGCAGACAGGTCCGGGTTAGCTTTATCAGCCACTTCCA                                                                   <  SRR4302547.426673/94‑1 (MQ=60)

                            AACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCA                                                                   >  SRR4302547.512767/1‑94 (MQ=60)

                            AACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCA                                                                   >  SRR4302547.2441412/1‑94 (MQ=60)

                            AACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCA                                                                   <  SRR4302547.5547020/94‑1 (MQ=60)

                            AACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCA                                                                   >  SRR4302547.5560447/1‑94 (MQ=60)

                             ACTTACGCACAACGTCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGCTAGCTTTATCAGCCACTTCCAG                                                                  >  SRR4302547.5773907/1‑94 (MQ=60)

                             ACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAG                                                                  <  SRR4302547.804248/94‑1 (MQ=60)

                              CTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGC                                                                 <  SRR4302547.2655600/94‑1 (MQ=60)

                              CTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGC                                                                 <  SRR4302547.894121/94‑1 (MQ=60)

                               TTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCG                                                                <  SRR4302547.848432/94‑1 (MQ=60)

                               TTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCG                                                                <  SRR4302547.4983883/94‑1 (MQ=60)

                                   GCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACG                                                            <  SRR4302547.2194634/94‑1 (MQ=60)

                                    CACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGA                                                           <  SRR4302547.1351010/94‑1 (MQ=60)

                                    CACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGA                                                           <  SRR4302547.2016665/94‑1 (MQ=60)

                                     ACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGAT                                                          >  SRR4302547.603108/1‑94 (MQ=60)

                                     ACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGAT                                                          <  SRR4302547.1576656/94‑1 (MQ=60)

                                      CAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATC                                                         >  SRR4302547.6665833/1‑94 (MQ=60)

                                       AACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCC                                                        >  SRR4302547.1776806/1‑94 (MQ=60)

                                       AACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCC                                                        >  SRR4302547.4888037/1‑94 (MQ=60)

                                         CATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAG                                                      >  SRR4302547.6329059/1‑94 (MQ=60)

                                         CATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAG                                                      <  SRR4302547.2035235/94‑1 (MQ=60)

                                          ATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGG                                                     >  SRR4302547.4597027/1‑94 (MQ=60)

                                          ACCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGG                                                     <  SRR4302547.659656/94‑1 (MQ=60)

                                             CAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTA                                                  >  SRR4302547.2624808/1‑94 (MQ=60)

                                              AGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTAT                                                 >  SRR4302547.2918302/1‑94 (MQ=60)

                                                CATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCA                                               >  SRR4302547.4255850/1‑94 (MQ=60)

                                                 ATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAA                                              <  SRR4302547.141421/94‑1 (MQ=60)

                                                 ATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAA                                              >  SRR4302547.58430/1‑94 (MQ=60)

                                                 ATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAA                                              >  SRR4302547.4560318/1‑94 (MQ=60)

                                                 ATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAA                                              <  SRR4302547.6643511/94‑1 (MQ=60)

                                                 ATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAA                                              >  SRR4302547.4502100/1‑94 (MQ=60)

                                                 ATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAA                                              >  SRR4302547.1908456/1‑94 (MQ=60)

                                                   CGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATC                                            <  SRR4302547.4959240/94‑1 (MQ=60)

                                                   CGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTACAGCGCACGATCCAGGCTATCAATC                                            >  SRR4302547.5137930/1‑94 (MQ=60)

                                                   CGACTTCAACGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCATGATCCAGGCTATCAATC                                            >  SRR4302547.6442956/1‑94 (MQ=60)

                                                    GACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCA                                           >  SRR4302547.1348844/1‑94 (MQ=60)

                                                    GACTTCAGCGACAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCA                                           <  SRR4302547.3833458/94‑1 (MQ=60)

                                                     ACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCAC                                          >  SRR4302547.3605482/1‑94 (MQ=60)

                                                      CTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACC                                         >  SRR4302547.2186726/1‑94 (MQ=60)

                                                      CTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACC                                         <  SRR4302547.309371/94‑1 (MQ=60)

                                                       TTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCG                                        >  SRR4302547.454321/1‑94 (MQ=60)

                                                       TTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCG                                        <  SRR4302547.601365/94‑1 (MQ=60)

                                                        TCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGG                                       >  SRR4302547.1441805/1‑94 (MQ=60)

                                                        TCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGG                                       <  SRR4302547.5693932/94‑1 (MQ=60)

                                                         CAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGC                                      <  SRR4302547.5095328/94‑1 (MQ=60)

                                                         CAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGC                                      <  SRR4302547.4921851/94‑1 (MQ=60)

                                                          AGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCA                                     <  SRR4302547.2328683/94‑1 (MQ=60)

                                                          AGCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCA                                     >  SRR4302547.1539223/1‑94 (MQ=60)

                                                           GCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAG                                    >  SRR4302547.2773554/1‑94 (MQ=60)

                                                           GCGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGGTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGTCTATCAcgcaccggcgt                                    >  SRR4302547.6586508/1‑83 (MQ=60)

                                                            CGTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGC                                   >  SRR4302547.6772021/1‑94 (MQ=60)

                                                             GTCAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCA                                  >  SRR4302547.273302/1‑94 (MQ=60)

                                                               CAGCTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAAT                                >  SRR4302547.2162547/1‑94 (MQ=60)

                                                                  CTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCG                             >  SRR4302547.2132270/1‑94 (MQ=60)

                                                                  CTCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCG                             >  SRR4302547.5781759/1‑94 (MQ=60)

                                                                   TCAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGT                            >  SRR4302547.6157331/1‑94 (MQ=60)

                                                                    CAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTT                           >  SRR4302547.374975/1‑94 (MQ=60)

                                                                    CAATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTT                           >  SRR4302547.4040941/1‑94 (MQ=60)

                                                                     AATGCCTTCAACCATCGCAGACATGTCCGGGTTCGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTG                          <  SRR4302547.6230719/94‑1 (MQ=60)

                                                                     AATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTG                          <  SRR4302547.5304068/94‑1 (MQ=60)

                                                                     AATGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTG                          >  SRR4302547.1514102/1‑94 (MQ=60)

                                                                       TGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGAT                        <  SRR4302547.4253940/94‑1 (MQ=60)

                                                                       TGCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGAT                        >  SRR4302547.854478/1‑94 (MQ=60)

                                                                        GCCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATG                       >  SRR4302547.93045/1‑94 (MQ=60)

                                                                         CCTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGA                      >  SRR4302547.6152433/1‑94 (MQ=60)

                                                                          CTTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAA                     >  SRR4302547.3462351/1‑94 (MQ=60)

                                                                           TTCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAAT                    >  SRR4302547.4979721/1‑94 (MQ=60)

                                                                            TCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATT                   >  SRR4302547.1438657/1‑94 (MQ=60)

                                                                            TCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATT                   >  SRR4302547.275883/1‑94 (MQ=60)

                                                                            TCAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATT                   >  SRR4302547.3510066/1‑94 (MQ=60)

                                                                             CAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTT                  >  SRR4302547.1155764/1‑94 (MQ=60)

                                                                             CAACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTT                  >  SRR4302547.5051619/1‑94 (MQ=60)

                                                                              AACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTC                 >  SRR4302547.6655285/1‑94 (MQ=60)

                                                                              AACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTC                 <  SRR4302547.2286117/94‑1 (MQ=60)

                                                                              AACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTC                 >  SRR4302547.5683925/1‑94 (MQ=60)

                                                                               ACCATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCT                <  SRR4302547.2363891/94‑1 (MQ=60)

                                                                                 CATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCC              <  SRR4302547.5521873/94‑1 (MQ=60)

                                                                                  ATCGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCA             <  SRR4302547.298286/94‑1 (MQ=60)

                                                                                    CGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGC           <  SRR4302547.6353357/94‑1 (MQ=60)

                                                                                    CGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGC           >  SRR4302547.5055806/1‑94 (MQ=60)

                                                                                    CGCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGC           >  SRR4302547.880463/1‑94 (MQ=60)

                                                                                     GCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCG          >  SRR4302547.2401824/1‑94 (MQ=60)

                                                                                     GCAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCG          <  SRR4302547.5105854/94‑1 (MQ=60)

                                                                                      CAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGC         <  SRR4302547.4848840/94‑1 (MQ=60)

                                                                                      CAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGC         >  SRR4302547.2149496/1‑94 (MQ=60)

                                                                                      CAGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGC         <  SRR4302547.857938/94‑1 (MQ=60)

                                                                                       AGACATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCG        >  SRR4302547.4334908/1‑94 (MQ=60)

                                                                                          CATGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAAT     <  SRR4302547.209915/94‑1 (MQ=60)

                                                                                            TGTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTT   >  SRR4302547.3047203/1‑94 (MQ=60)

                                                                                             GTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGCATTTG  >  SRR4302547.32218/1‑94 (MQ=60)

                                                                                             GTCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTG  >  SRR4302547.1128753/1‑94 (MQ=60)

GTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCATCGACTTCAGCGTCAGCTCAATGCCTTCAACCATCGCAGACATATCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGATCCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTG  >  NC_000913/2748292‑2748478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`