Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,801,986 T→C V155A (GTG→GCG)  nrdF → ribonucleoside‑diphosphate reductase 2 beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,801,9860TC88.6% 672.8 / 61.7 237V155A (GTG→GCG) nrdFribonucleoside‑diphosphate reductase 2 beta chain
Reads supporting (aligned to +/- strand):  ref base T (12/15);  new base C (116/94);  total (128/109)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 249 total aligned reads displayed.

AGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATC  >  NC_000913/2801895‑2802077
                                                                                           |                                                                                           
  tGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTTAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGtt                                                                                          >  1:4615643/1‑93 (MQ=255)
  tGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGtt                                                                                          >  1:4205495/1‑93 (MQ=255)
  tGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGtt                                                                                          >  2:5675911/1‑93 (MQ=255)
  tGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGtt                                                                                          >  2:6135024/1‑93 (MQ=255)
   gaagaaAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGttt                                                                                         >  2:6625278/1‑93 (MQ=255)
    aagaaAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTc                                                                                        <  1:3230605/93‑1 (MQ=255)
    aagaaAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTc                                                                                        >  2:4386754/1‑93 (MQ=255)
     agaaAACGCACCGTTGCGGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCt                                                                                       <  2:3601799/93‑1 (MQ=255)
     agaaAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCt                                                                                       <  1:1846872/93‑1 (MQ=255)
     agaaAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGAGAGTTTCt                                                                                       >  1:2887676/1‑93 (MQ=255)
       aaaaCGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTg                                                                                     <  2:2181350/93‑1 (MQ=255)
         aaCGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGaa                                                                                   <  1:3951891/93‑1 (MQ=255)
            gCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCt                                                                                >  2:5838424/1‑93 (MQ=255)
             cACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCtt                                                                               <  1:3801050/93‑1 (MQ=255)
              aCCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCttt                                                                              >  2:3077698/1‑93 (MQ=255)
                cGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCttttt                                                                            >  1:3309297/1‑93 (MQ=255)
                cGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCttttt                                                                            <  2:235546/93‑1 (MQ=255)
                cGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCttttt                                                                            <  2:455750/93‑1 (MQ=255)
                 gTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCtttttt                                                                           <  2:1816542/93‑1 (MQ=255)
                 gTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCtttttt                                                                           >  1:5850485/1‑93 (MQ=255)
                 gTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCtttttt                                                                           <  2:787006/93‑1 (MQ=255)
                 gTTGCAGCGAAAAGCTCAGATGATTCAGCACCATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCtttttt                                                                           <  1:4974929/93‑1 (MQ=255)
                  ttGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTg                                                                          <  2:1010723/93‑1 (MQ=255)
                   tGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGt                                                                         <  2:1154590/93‑1 (MQ=255)
                    gCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGtt                                                                        <  2:5134274/93‑1 (MQ=255)
                    gCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGtt                                                                        <  1:1769385/93‑1 (MQ=255)
                       gCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTa                                                                     >  1:2623594/1‑93 (MQ=255)
                        cGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTAt                                                                    <  1:479461/93‑1 (MQ=255)
                         gAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTAtt                                                                   >  2:257093/1‑93 (MQ=255)
                         gAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTAtt                                                                   >  2:5044400/1‑93 (MQ=255)
                         gAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTAtt                                                                   <  2:3795133/93‑1 (MQ=255)
                          aaaaGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTc                                                                  >  2:3035334/1‑93 (MQ=255)
                          aaaaGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTc                                                                  <  2:5368615/93‑1 (MQ=255)
                          aaaaGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTc                                                                  >  2:3887040/1‑93 (MQ=255)
                           aaaGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTcc                                                                 <  2:1608446/93‑1 (MQ=255)
                           aaaGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTcc                                                                 <  2:5334908/93‑1 (MQ=255)
                               cTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGtt                                                             <  2:2879925/93‑1 (MQ=255)
                               cTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGtt                                                             >  2:120099/1‑93 (MQ=255)
                                  aGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCt                                                          <  2:3386083/93‑1 (MQ=255)
                                   gATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTg                                                         <  1:1176954/93‑1 (MQ=255)
                                    attattCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTgg                                                        >  1:2338436/1‑93 (MQ=255)
                                     ttattCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGc                                                       <  2:6278482/93‑1 (MQ=255)
                                     atattCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGc                                                       <  2:1710243/92‑1 (MQ=255)
                                      tattCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCt                                                      >  1:5603735/1‑93 (MQ=255)
                                      tattCAGCAACATTATCGCGGAGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCt                                                      >  1:6633577/1‑93 (MQ=255)
                                       attCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTg                                                     <  2:4201080/93‑1 (MQ=255)
                                        ttCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGc                                                    <  1:838583/93‑1 (MQ=255)
                                           aGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGa                                                 >  2:6503143/1‑93 (MQ=255)
                                           aGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGa                                                 >  1:2290534/1‑93 (MQ=255)
                                           aGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGa                                                 >  2:6321316/1‑93 (MQ=255)
                                            gCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGAt                                                <  2:4733841/93‑1 (MQ=255)
                                               aCATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTa                                             <  1:4128166/93‑1 (MQ=255)
                                                cATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAt                                            >  2:5925333/1‑93 (MQ=255)
                                                 aTTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAtt                                           >  1:5238514/1‑93 (MQ=255)
                                                 aTTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAtt                                           >  1:372497/1‑93 (MQ=255)
                                                 aTTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAtt                                           <  2:6562074/93‑1 (MQ=255)
                                                  ttATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAttt                                          >  2:4845493/1‑93 (MQ=255)
                                                     tCGCGGTTATGAGCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTc                                       <  2:2791101/93‑1 (MQ=255)
                                                      cgcgGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTATTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTcc                                      >  2:4673082/1‑93 (MQ=255)
                                                       gcgGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCa                                     >  1:2711908/1‑93 (MQ=255)
                                                        cgGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAg                                    >  1:4096156/1‑93 (MQ=255)
                                                        cgGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAg                                    >  1:6211771/1‑93 (MQ=255)
                                                         ggTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGc                                   >  1:2046175/1‑93 (MQ=255)
                                                          gTGATGATCCGCTTAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGcc                                  <  2:1954468/93‑1 (MQ=255)
                                                          gTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGcc                                  >  1:356527/1‑93 (MQ=255)
                                                          gTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGcc                                  <  1:1514518/93‑1 (MQ=255)
                                                          gTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGcc                                  >  2:5470003/1‑93 (MQ=255)
                                                          gTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGcc                                  >  2:3584572/1‑93 (MQ=255)
                                                           tgatgaTCCGCTGAAAAAGAAAATCGCCAGTTCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCcg                                 <  1:4478243/93‑1 (MQ=255)
                                                            gatgatCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCcgc                                <  1:952691/93‑1 (MQ=255)
                                                            gatgatCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCcgc                                <  1:1052337/93‑1 (MQ=255)
                                                            gatgatCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCcgc                                <  1:5463389/93‑1 (MQ=255)
                                                              tgatCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCgg                              <  2:1483985/93‑1 (MQ=255)
                                                              tgatCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCgg                              <  1:5894918/93‑1 (MQ=255)
                                                               gatCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGa                             >  2:6267999/1‑93 (MQ=255)
                                                               gatCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGa                             >  1:5051307/1‑93 (MQ=255)
                                                                atCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGaa                            <  1:4278254/93‑1 (MQ=255)
                                                                atCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGaa                            >  1:1990126/1‑93 (MQ=255)
                                                                atCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGaa                            >  1:5156700/1‑93 (MQ=255)
                                                                  ccGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAg                          >  1:757822/1‑93 (MQ=255)
                                                                    gCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCt                        <  1:802778/93‑1 (MQ=255)
                                                                      tGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGa                      >  2:6052589/1‑93 (MQ=255)
                                                                       gAAAAAGAAAATGGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGAc                     <  2:4113636/93‑1 (MQ=255)
                                                                          aaaGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCaa                  >  1:4334532/1‑93 (MQ=255)
                                                                           aaGAAAAGCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAAt                 <  2:3227649/93‑1 (MQ=255)
                                                                            aGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATa                >  2:248442/1‑93 (MQ=255)
                                                                             gAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATAc               <  2:1761946/93‑1 (MQ=255)
                                                                                aaTCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACcgc            >  1:5369417/1‑93 (MQ=255)
                                                                                aaTCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACcgc            >  1:4580628/1‑93 (MQ=255)
                                                                                aaTCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACcgc            <  1:4419005/93‑1 (MQ=255)
                                                                                aaTCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACcgc            >  1:6308546/1‑93 (MQ=255)
                                                                                aaTCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACcgc            >  1:1694642/1‑93 (MQ=255)
                                                                                  tCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCTGCGGAAAGCTGACCAATACCGCgg          <  2:4077623/93‑1 (MQ=255)
                                                                                   cGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGa         >  1:6108305/1‑93 (MQ=255)
                                                                                     ccAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGAcc       <  1:499651/93‑1 (MQ=255)
                                                                                     ccAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGAcc       <  1:3005617/93‑1 (MQ=255)
                                                                                      cAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCt      >  1:5737182/1‑93 (MQ=255)
                                                                                       aGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTg     >  2:93002/1‑93 (MQ=255)
                                                                                        gtgcgtTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGa    >  1:1314203/1‑93 (MQ=255)
                                                                                          gtgtTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATc  <  1:4509949/93‑1 (MQ=255)
                                                                                           |                                                                                           
AGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATC  >  NC_000913/2801895‑2802077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCG  >  NC_000913/2801893‑2802079
                                                                                             |                                                                                             
TGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGC                                                                                               >  SRR4302547.368247/1‑94 (MQ=60)
GGAGTGAAGAAAACGCACCGTTGCTGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGC                                                                                               <  SRR4302547.6655797/94‑1 (MQ=60)
GGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGC                                                                                               <  SRR4302547.619736/94‑1 (MQ=60)
 GAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTG                                                                                              >  SRR4302547.4836479/1‑94 (MQ=60)
 GAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCG                                                                                              <  SRR4302547.5199266/94‑1 (MQ=60)
   GTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTTAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTT                                                                                            >  SRR4302547.4711208/1‑94 (MQ=60)
   GTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTT                                                                                            >  SRR4302547.4291304/1‑94 (MQ=60)
     GAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTC                                                                                          <  SRR4302547.423860/94‑1 (MQ=60)
      AAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCT                                                                                         <  SRR4302547.3295082/94‑1 (MQ=60)
      AAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCT                                                                                         <  SRR4302547.4465310/94‑1 (MQ=60)
      AAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGAGAGTTTCT                                                                                         >  SRR4302547.2945289/1‑94 (MQ=60)
       AGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTT                                                                                        <  SRR4302547.1883385/94‑1 (MQ=60)
         AAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGA                                                                                      >  SRR4302547.2256562/1‑94 (MQ=60)
           AACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAAT                                                                                    <  SRR4302547.4032263/94‑1 (MQ=60)
           AACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAAT                                                                                    <  SRR4302547.3856069/94‑1 (MQ=60)
            ACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATC                                                                                   <  SRR4302547.1324780/94‑1 (MQ=60)
             CGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAGAGAAAATCGCCAGTGCGTTTCTTGAATCT                                                                                  >  SRR4302547.2018168/1‑94 (MQ=60)
             CGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCT                                                                                  <  SRR4302547.4650241/94‑1 (MQ=60)
             CGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCT                                                                                  <  SRR4302547.2688020/94‑1 (MQ=60)
               CACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTT                                                                                >  SRR4302547.1832450/1‑94 (MQ=60)
               CACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTT                                                                                <  SRR4302547.5081882/94‑1 (MQ=60)
               CACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTT                                                                                <  SRR4302547.3878525/94‑1 (MQ=60)
               CACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTT                                                                                >  SRR4302547.2046157/1‑94 (MQ=60)
                atcggTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGTTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTCGAATCTTTT                                                                               <  SRR4302547.3948759/89‑1 (MQ=60)
                ACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTT                                                                               >  SRR4302547.6081362/1‑94 (MQ=60)
                ACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTT                                                                               >  SRR4302547.2413466/1‑94 (MQ=60)
                 CCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTT                                                                              >  SRR4302547.3376024/1‑94 (MQ=60)
                  CGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTT                                                                             >  SRR4302547.5971510/1‑94 (MQ=60)
                   GTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTG                                                                            >  SRR4302547.4989345/1‑94 (MQ=60)
                   GTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTG                                                                            >  SRR4302547.2279026/1‑94 (MQ=60)
                   GTTGCAGCGAAAAGCTCAGATGATTCAGCACCATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTG                                                                            <  SRR4302547.5077838/94‑1 (MQ=60)
                     TGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTT                                                                          <  SRR4302547.919236/94‑1 (MQ=60)
                      GCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTC                                                                         <  SRR4302547.1804470/94‑1 (MQ=60)
                      GCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTC                                                                         <  SRR4302547.1398395/94‑1 (MQ=60)
                       CAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCT                                                                        >  SRR4302547.5930916/1‑94 (MQ=60)
                        AGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTA                                                                       >  SRR4302547.2676448/1‑94 (MQ=60)
                        AGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTA                                                                       >  SRR4302547.1966690/1‑94 (MQ=60)
                          CGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAACGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATT                                                                     <  SRR4302547.5516009/94‑1 (MQ=60)
                          CGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATT                                                                     <  SRR4302547.488270/94‑1 (MQ=60)
                           GAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTC                                                                    >  SRR4302547.827828/1‑94 (MQ=60)
                           GAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTC                                                                    <  SRR4302547.4184513/94‑1 (MQ=60)
                           AAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTC                                                                    <  SRR4302547.5193959/94‑1 (MQ=60)
                            AAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCC                                                                   <  SRR4302547.6460071/94‑1 (MQ=60)
                            AAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCC                                                                   >  SRR4302547.4623825/1‑94 (MQ=60)
                             AAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCG                                                                  <  SRR4302547.1170591/94‑1 (MQ=60)
                              AACCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGG                                                                 <  SRR4302547.4368551/94‑1 (MQ=60)
                               AGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGT                                                                <  SRR4302547.5148543/94‑1 (MQ=60)
                               AGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGT                                                                >  SRR4302547.6254453/1‑94 (MQ=60)
                               AGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGT                                                                >  SRR4302547.733961/1‑94 (MQ=60)
                                GCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTT                                                               <  SRR4302547.3235012/94‑1 (MQ=60)
                                GCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTT                                                               >  SRR4302547.5694485/1‑94 (MQ=60)
                                GCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTT                                                               >  SRR4302547.2848863/1‑94 (MQ=60)
                                 CTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTT                                                              >  SRR4302547.4008269/1‑94 (MQ=60)
                                 CTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTT                                                              <  SRR4302547.3358889/94‑1 (MQ=60)
                                 CTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTT                                                              <  SRR4302547.485668/94‑1 (MQ=60)
                                  TCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTC                                                             >  SRR4302547.1689768/1‑94 (MQ=60)
                                  TCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTC                                                             >  SRR4302547.4405052/1‑94 (MQ=60)
                                    AGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTG                                                           <  SRR4302547.386168/94‑1 (MQ=60)
                                    AGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTG                                                           <  SRR4302547.3745342/94‑1 (MQ=60)
                                    AGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTG                                                           <  SRR4302547.806006/94‑1 (MQ=60)
                                     GATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGG                                                          >  SRR4302547.2385844/1‑94 (MQ=60)
                                     GATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGG                                                          <  SRR4302547.1200732/94‑1 (MQ=60)
                                     GATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGG                                                          >  SRR4302547.5668602/1‑94 (MQ=60)
                                      ATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGC                                                         >  SRR4302547.6344595/1‑94 (MQ=60)
                                       TTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCT                                                        >  SRR4302547.5719340/1‑94 (MQ=60)
                                       TTATTCAGCAACATTATCGCGGAGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCT                                                        >  SRR4302547.6771658/1‑94 (MQ=60)
                                          TTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCC                                                     <  SRR4302547.854579/94‑1 (MQ=60)
                                            CAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGA                                                   >  SRR4302547.2336941/1‑94 (MQ=60)
                                                 ACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAT                                              <  SRR4302547.4212131/94‑1 (MQ=60)
                                                  CATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATT                                             >  SRR4302547.379399/1‑94 (MQ=60)
                                                  CATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATT                                             <  SRR4302547.4324673/94‑1 (MQ=60)
                                                  CATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATT                                             >  SRR4302547.5346353/1‑94 (MQ=60)
                                                   ATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTT                                            <  SRR4302547.4214712/94‑1 (MQ=60)
                                                   ATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTT                                            <  SRR4302547.2954912/94‑1 (MQ=60)
                                                      ATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTC                                         >  SRR4302547.5805362/1‑94 (MQ=60)
                                                        CGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCA                                       <  SRR4302547.4567295/94‑1 (MQ=60)
                                                        CGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCA                                       >  SRR4302547.2766352/1‑94 (MQ=60)
                                                         GCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAG                                      <  SRR4302547.2363141/94‑1 (MQ=60)
                                                         GCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAG                                      >  SRR4302547.4179454/1‑94 (MQ=60)
                                                         GCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAG                                      >  SRR4302547.6340763/1‑94 (MQ=60)
                                                          CGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGC                                     >  SRR4302547.2086990/1‑94 (MQ=60)
                                                           GGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCC                                    >  SRR4302547.323291/1‑94 (MQ=60)
                                                           GGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCC                                    >  SRR4302547.363137/1‑94 (MQ=60)
                                                           CGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCTTTTCTTGAATCTTTTTTTTTCTATTCCGGTTTCTGGCTGCCGTTGTATTTTTCCAGCC                                    <  SRR4302547.1788629/94‑1 (MQ=60)
                                                            GTGATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCG                                   <  SRR4302547.1544697/94‑1 (MQ=60)
                                                             TGATGATCCGCTGAAAAAGAAAATCGCCAGTTCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGC                                  <  SRR4302547.4570760/94‑1 (MQ=60)
                                                              GATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCG                                 <  SRR4302547.5576248/94‑1 (MQ=60)
                                                              GATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCG                                 <  SRR4302547.1073230/94‑1 (MQ=60)
                                                              GATGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCG                                 <  SRR4302547.971193/94‑1 (MQ=60)
                                                                TGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGA                               >  SRR4302547.5155595/1‑94 (MQ=60)
                                                                TGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGA                               <  SRR4302547.533403/94‑1 (MQ=60)
                                                                TGATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGA                               <  SRR4302547.6016979/94‑1 (MQ=60)
                                                                 GATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAA                              >  SRR4302547.2029652/1‑94 (MQ=60)
                                                                 GATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAA                              >  SRR4302547.5237693/1‑94 (MQ=60)
                                                                 GATCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAA                              >  SRR4302547.5262883/1‑94 (MQ=60)
                                                                  ATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAA                             <  SRR4302547.4366119/94‑1 (MQ=60)
                                                                   TCCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAG                            >  SRR4302547.772106/1‑94 (MQ=60)
                                                                    CCGCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGC                           <  SRR4302547.1539089/94‑1 (MQ=60)
                                                                      GCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTG                         >  SRR4302547.4678920/1‑94 (MQ=60)
                                                                      GCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTG                         >  SRR4302547.4580824/1‑94 (MQ=60)
                                                                      GCTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTG                         <  SRR4302547.817990/94‑1 (MQ=60)
                                                                      CCTGAAAAAGAACGTCGCCACTGCGTTACTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCGGATGTATTTTTCCAGCCGCGGAAAGCTG                         <  SRR4302547.6008652/94‑1 (MQ=60)
                                                                       CTGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGA                        >  SRR4302547.507216/1‑94 (MQ=60)
                                                                        TGAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGAC                       >  SRR4302547.3603424/1‑94 (MQ=60)
                                                                         GAAAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACC                      <  SRR4302547.852569/94‑1 (MQ=60)
                                                                           AAAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAA                    >  SRR4302547.4423701/1‑94 (MQ=60)
                                                                            AAAGAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAAT                   >  SRR4302547.6379410/1‑94 (MQ=60)
                                                                             AAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATA                  >  SRR4302547.4145955/1‑94 (MQ=60)
                                                                               GAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACC                <  SRR4302547.452168/94‑1 (MQ=60)
                                                                               GAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACC                >  SRR4302547.5287526/1‑94 (MQ=60)
                                                                               GAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACC                <  SRR4302547.1797929/94‑1 (MQ=60)
                                                                               GAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACC                >  SRR4302547.757628/1‑94 (MQ=60)
                                                                               GAAAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACC                <  SRR4302547.5434410/94‑1 (MQ=60)
                                                                                 AAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGC              >  SRR4302547.5480224/1‑94 (MQ=60)
                                                                                 AAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGC              >  SRR4302547.1727968/1‑94 (MQ=60)
                                                                                 AAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGC              >  SRR4302547.6439698/1‑94 (MQ=60)
                                                                                 AAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGC              >  SRR4302547.2783095/1‑94 (MQ=60)
                                                                                 AAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGC              >  SRR4302547.2609346/1‑94 (MQ=60)
                                                                                 AAATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGC              >  SRR4302547.4675427/1‑94 (MQ=60)
                                                                                  AATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCG             >  SRR4302547.6144414/1‑94 (MQ=60)
                                                                                  AATCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCG             <  SRR4302547.4510218/94‑1 (MQ=60)
                                                                                    TCGCCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGA           >  SRR4302547.6235009/1‑94 (MQ=60)
                                                                                       CCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCT        <  SRR4302547.508760/94‑1 (MQ=60)
                                                                                       CCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCT        >  SRR4302547.5855720/1‑94 (MQ=60)
                                                                                       CCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCT        <  SRR4302547.3463113/94‑1 (MQ=60)
                                                                                       CCAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCT        <  SRR4302547.3065392/94‑1 (MQ=60)
                                                                                        CAGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTG       <  SRR4302547.4056181/94‑1 (MQ=60)
                                                                                         AGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGA      >  SRR4302547.1340622/1‑94 (MQ=60)
                                                                                         AGTGCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGA      >  SRR4302547.5914765/1‑94 (MQ=60)
                                                                                            GTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCC   <  SRR4302547.4603125/94‑1 (MQ=60)
                                                                                            GCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCC   <  SRR4302547.4240439/94‑1 (MQ=60)
                                                                                            GCGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCC   <  SRR4302547.5154928/94‑1 (MQ=60)
                                                                                             CGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCG  >  SRR4302547.5871438/1‑94 (MQ=60)
                                                                                             CGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCG  <  SRR4302547.5786011/94‑1 (MQ=60)
                                                                                             CGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCG  >  SRR4302547.4988282/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCG  >  NC_000913/2801893‑2802079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑