Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,803,648 T→C S271S (AGT→AGC)  proV → ATP‑binding component of transport system for glycine, betaine and proline

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,803,6480TC100.0% 933.8 / NA 250S271S (AGT→AGC) proVATP‑binding component of transport system for glycine, betaine and proline
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (118/132);  total (118/132)

Only 100 of 250 total aligned reads displayed.

TGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAA  >  NC_000913/2803558‑2803737
                                                                                          |                                                                                         
tGAAGTGGTACAGGTCGTCACACCGGATGAAATTCCCAATAATCCGGCGAATGATTATGTCCGTCCCTGCTTCCGTGGCGTTGATATTAGCCa                                                                                         <  1:4462849/93‑1 (MQ=255)
tGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCa                                                                                         <  2:5523272/93‑1 (MQ=255)
 gAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAg                                                                                        >  1:3184010/1‑93 (MQ=255)
 gAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAg                                                                                        <  2:1919017/93‑1 (MQ=255)
 gAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAg                                                                                        <  2:3823258/93‑1 (MQ=255)
  aaGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAgg                                                                                       <  1:5799167/93‑1 (MQ=255)
   aGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGt                                                                                      >  1:2574758/1‑93 (MQ=255)
   aGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGt                                                                                      <  1:4196422/93‑1 (MQ=255)
   aGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGt                                                                                      >  1:4702450/1‑93 (MQ=255)
   aGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGt                                                                                      <  2:6426976/93‑1 (MQ=255)
     tGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTAt                                                                                    <  2:3042942/93‑1 (MQ=255)
     tGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTAt                                                                                    <  1:5387955/93‑1 (MQ=255)
     tGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTAt                                                                                    >  1:2911650/1‑93 (MQ=255)
       gTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTc                                                                                  >  2:3955403/1‑93 (MQ=255)
        tACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCa                                                                                 <  2:1624522/93‑1 (MQ=255)
        tACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCa                                                                                 <  1:1514242/93‑1 (MQ=255)
        tACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCa                                                                                 <  1:1920049/93‑1 (MQ=255)
        tACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCa                                                                                 >  2:2068419/1‑93 (MQ=255)
         aCAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAg                                                                                >  2:1644178/1‑93 (MQ=255)
          cAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGt                                                                               >  2:5721568/1‑93 (MQ=255)
           aGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTg                                                                              >  2:1300454/1‑93 (MQ=255)
            ggTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGc                                                                             >  1:1453595/1‑93 (MQ=255)
             gTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCg                                                                            >  2:151862/1‑93 (MQ=255)
             gTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCg                                                                            <  2:2519370/93‑1 (MQ=255)
             gTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCg                                                                            >  2:863295/1‑93 (MQ=255)
                ggCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGaaa                                                                         <  2:105699/93‑1 (MQ=255)
                    cacCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGata                                                                     <  2:2733644/93‑1 (MQ=255)
                     acCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGatat                                                                    >  1:1578633/1‑93 (MQ=255)
                     acCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGatat                                                                    <  1:4894858/93‑1 (MQ=255)
                       cGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTg                                                                  <  2:3249768/93‑1 (MQ=255)
                       cGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTg                                                                  <  1:4336761/93‑1 (MQ=255)
                         gATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGcc                                                                >  2:4541543/1‑93 (MQ=255)
                          aTGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGccc                                                               <  2:3660994/93‑1 (MQ=255)
                           tGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCccg                                                              >  2:4918771/1‑93 (MQ=255)
                            gAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCccgc                                                             <  2:3512577/93‑1 (MQ=255)
                             aaaTTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCccgcc                                                            <  1:4678220/93‑1 (MQ=255)
                             aaaTTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCccgcc                                                            <  2:6295861/93‑1 (MQ=255)
                              aaTTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCccgccg                                                           >  1:2340001/1‑93 (MQ=255)
                               aTTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCgg                                                          <  2:3925341/93‑1 (MQ=255)
                                ttCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGa                                                         >  1:3915752/1‑93 (MQ=255)
                                ttCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGa                                                         >  1:3690636/1‑93 (MQ=255)
                                 tctcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGac                                                        <  2:4755628/93‑1 (MQ=255)
                                  ctcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGaca                                                       <  1:177413/93‑1 (MQ=255)
                                  ctcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGaca                                                       <  2:4494430/93‑1 (MQ=255)
                                   tcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGCAAGATATTGCCCGCCGGacac                                                      >  1:3062077/1‑93 (MQ=255)
                                   tcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGacac                                                      <  2:2187165/93‑1 (MQ=255)
                                   tcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGacac                                                      >  1:817866/1‑93 (MQ=255)
                                   tcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGacac                                                      <  1:2922802/93‑1 (MQ=255)
                                   tcAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGacac                                                      <  2:704192/93‑1 (MQ=255)
                                    cAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACAcc                                                     >  1:5552019/1‑93 (MQ=255)
                                    cAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACAcc                                                     <  1:4067863/93‑1 (MQ=255)
                                      ataatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGa                                                   >  1:6280222/1‑93 (MQ=255)
                                      ataatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGa                                                   >  1:1046916/1‑93 (MQ=255)
                                       taatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGaa                                                  <  2:428545/93‑1 (MQ=255)
                                       taatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGaa                                                  <  2:6022563/93‑1 (MQ=255)
                                        aatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAAt                                                 <  2:811942/93‑1 (MQ=255)
                                        aatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAAt                                                 <  1:5192857/93‑1 (MQ=255)
                                         atCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATg                                                >  2:6486980/1‑93 (MQ=255)
                                          tCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATgg                                               >  1:2408447/1‑93 (MQ=255)
                                           ccGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGc                                              <  2:6347882/93‑1 (MQ=255)
                                             ggCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCtt                                            >  1:2144076/1‑93 (MQ=255)
                                             ggCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCtt                                            <  2:5452308/93‑1 (MQ=255)
                                                gAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAAt                                         >  2:2555755/1‑93 (MQ=255)
                                                  aTGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTc                                       >  1:4600119/1‑93 (MQ=255)
                                                   tGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGCATGGCTTAATTCg                                      >  1:6200831/1‑93 (MQ=255)
                                                        aTGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTaaaa                                 >  1:6418839/1‑93 (MQ=255)
                                                          gTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAAcc                               <  2:3442321/93‑1 (MQ=255)
                                                           tCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAAccc                              <  2:5790766/93‑1 (MQ=255)
                                                           tCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAAccc                              >  2:741253/1‑93 (MQ=255)
                                                            ccGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAAcccc                             <  1:3762179/93‑1 (MQ=255)
                                                            ccGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAAcccc                             <  1:3594367/93‑1 (MQ=255)
                                                            ccGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAAcccc                             >  2:404870/1‑93 (MQ=255)
                                                              gTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTg                           <  2:425016/93‑1 (MQ=255)
                                                               tACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTgg                          >  1:210835/1‑93 (MQ=255)
                                                                aCCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGc                         >  1:34207/1‑93 (MQ=255)
                                                                aCCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGc                         >  2:5311928/1‑93 (MQ=255)
                                                                aCCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGc                         <  1:3676383/93‑1 (MQ=255)
                                                                aCCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGc                         <  2:4993183/93‑1 (MQ=255)
                                                                  cttcttCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCtt                       <  2:1183785/93‑1 (MQ=255)
                                                                     cttcCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCgg                    <  2:5939355/93‑1 (MQ=255)
                                                                     cttcCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCgg                    <  1:3485665/93‑1 (MQ=255)
                                                                         cGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCa                >  1:5133495/1‑93 (MQ=255)
                                                                         cGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCa                >  2:5084246/1‑93 (MQ=255)
                                                                          gTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCAc               >  1:2471133/1‑93 (MQ=255)
                                                                            ggCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGt             >  1:3320253/1‑93 (MQ=255)
                                                                            ggCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGt             >  2:120163/1‑93 (MQ=255)
                                                                             gCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGtt            >  1:3273086/1‑93 (MQ=255)
                                                                             gCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGtt            <  2:4084645/93‑1 (MQ=255)
                                                                             gCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGtt            >  1:1278192/1‑93 (MQ=255)
                                                                               gTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCg          <  1:6161573/93‑1 (MQ=255)
                                                                               gTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCg          >  1:5703751/1‑93 (MQ=255)
                                                                                ttGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCgg         <  1:1632076/93‑1 (MQ=255)
                                                                                ttGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCgg         >  1:656515/1‑93 (MQ=255)
                                                                                ttGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCgg         >  2:196149/1‑93 (MQ=255)
                                                                                 tGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGc        >  1:4540446/1‑93 (MQ=255)
                                                                                  gATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCa       >  2:5277331/1‑93 (MQ=255)
                                                                                  gATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCa       >  1:5010944/1‑93 (MQ=255)
                                                                                  gATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCa       >  2:1135449/1‑93 (MQ=255)
                                                                                  gATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCa       >  1:2690905/1‑93 (MQ=255)
                                                                                  gATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCa       <  2:2681128/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAA  >  NC_000913/2803558‑2803737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTA  >  NC_000913/2803556‑2803741
                                                                                            |                                                                                             
GGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCC                                                                                              <  SRR4302547.223478/94‑1 (MQ=60)
 GTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCA                                                                                             <  SRR4302547.1333903/94‑1 (MQ=60)
 GTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCA                                                                                             <  SRR4302547.5081816/94‑1 (MQ=60)
  TGAAGTGGTACAGGTCGTCACACCGGATGAAATTCCCAATAATCCGGCGAATGATTATGTCCGTCCCTGCTTCCGTGGCGTTGATATTAGCCAG                                                                                            <  SRR4302547.4555077/94‑1 (MQ=60)
  TGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAG                                                                                            <  SRR4302547.6578903/94‑1 (MQ=60)
  TGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAG                                                                                            >  SRR4302547.3247460/1‑94 (MQ=60)
   GAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGG                                                                                           <  SRR4302547.2129014/94‑1 (MQ=60)
   GAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGG                                                                                           >  SRR4302547.3530215/1‑94 (MQ=60)
    AAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGT                                                                                          >  SRR4302547.4799839/1‑94 (MQ=60)
    AAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGT                                                                                          >  SRR4302547.2626729/1‑94 (MQ=60)
    AAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGT                                                                                          <  SRR4302547.5919130/94‑1 (MQ=60)
     AGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTA                                                                                         <  SRR4302547.4282006/94‑1 (MQ=60)
     AGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTA                                                                                         >  SRR4302547.6512198/1‑94 (MQ=60)
      GTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTAT                                                                                        >  SRR4302547.2969732/1‑94 (MQ=60)
       TGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATT                                                                                       <  SRR4302547.5499215/94‑1 (MQ=60)
         GTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCA                                                                                     >  SRR4302547.3520775/1‑94 (MQ=60)
          TACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAG                                                                                    >  SRR4302547.5142156/1‑94 (MQ=60)
          TACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAG                                                                                    <  SRR4302547.1544417/94‑1 (MQ=60)
          TACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAG                                                                                    <  SRR4302547.1958074/94‑1 (MQ=60)
            CAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCATTG                                                                                  >  SRR4302547.4054438/1‑94 (MQ=60)
            CAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTG                                                                                  >  SRR4302547.176874/1‑94 (MQ=60)
             AGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGC                                                                                 >  SRR4302547.5916213/1‑94 (MQ=60)
             AGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGC                                                                                 >  SRR4302547.1482655/1‑94 (MQ=60)
              GGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCG                                                                                >  SRR4302547.4856956/1‑94 (MQ=60)
              GGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCG                                                                                <  SRR4302547.4777655/94‑1 (MQ=60)
              GGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCG                                                                                <  SRR4302547.5324351/94‑1 (MQ=60)
               GTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGA                                                                               <  SRR4302547.160955/94‑1 (MQ=60)
               GTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGA                                                                               >  SRR4302547.5203756/1‑94 (MQ=60)
                    CACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGAT                                                                          <  SRR4302547.3256471/94‑1 (MQ=60)
                      CACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATAT                                                                        >  SRR4302547.3775166/1‑94 (MQ=60)
                      CACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATAT                                                                        <  SRR4302547.6129732/94‑1 (MQ=60)
                      CACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATAT                                                                        >  SRR4302547.1610047/1‑94 (MQ=60)
                      CACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGGATTCAGTGCGAAAGATAT                                                                        >  SRR4302547.4320338/1‑94 (MQ=60)
                      CACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGGATTCAGTGCGAAAGATAT                                                                        >  SRR4302547.3727374/1‑94 (MQ=60)
                       ACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATT                                                                       <  SRR4302547.4996234/94‑1 (MQ=60)
                       ACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATT                                                                       <  SRR4302547.19229/94‑1 (MQ=60)
                        CCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTG                                                                      <  SRR4302547.1513284/94‑1 (MQ=60)
                         CGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGC                                                                     <  SRR4302547.4425968/94‑1 (MQ=60)
                         CGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGC                                                                     >  SRR4302547.6747848/1‑94 (MQ=60)
                         CGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGC                                                                     <  SRR4302547.4688727/94‑1 (MQ=60)
                         CGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGC                                                                     <  SRR4302547.3372293/94‑1 (MQ=60)
                         CGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGC                                                                     <  SRR4302547.2660007/94‑1 (MQ=60)
                         CGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGC                                                                     >  SRR4302547.5769121/1‑94 (MQ=60)
                         CGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGC                                                                     >  SRR4302547.5259435/1‑94 (MQ=60)
                          GGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCC                                                                    >  SRR4302547.628382/1‑94 (MQ=60)
                           GATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCC                                                                   <  SRR4302547.3140548/94‑1 (MQ=60)
                            ATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCG                                                                  <  SRR4302547.4953457/94‑1 (MQ=60)
                              GAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCC                                                                <  SRR4302547.2454785/94‑1 (MQ=60)
                              GAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCC                                                                <  SRR4302547.3817318/94‑1 (MQ=60)
                               AAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCG                                                               <  SRR4302547.4775128/94‑1 (MQ=60)
                               AAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCG                                                               >  SRR4302547.2387435/1‑94 (MQ=60)
                                 ATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGA                                                             >  SRR4302547.3995370/1‑94 (MQ=60)
                                 ATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGA                                                             <  SRR4302547.6348343/94‑1 (MQ=60)
                                 ATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGA                                                             >  SRR4302547.3765982/1‑94 (MQ=60)
                                   TCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACA                                                           <  SRR4302547.6681927/94‑1 (MQ=60)
                                    CTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGCAAGATATTGCCCGCCGGACAC                                                          >  SRR4302547.3122977/1‑94 (MQ=60)
                                    CTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACAC                                                          >  SRR4302547.833407/1‑94 (MQ=60)
                                    CTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACAC                                                          <  SRR4302547.180709/94‑1 (MQ=60)
                                     TCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACC                                                         >  SRR4302547.5666564/1‑94 (MQ=60)
                                     TCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACC                                                         >  SRR4302547.1383815/1‑94 (MQ=60)
                                     TCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACC                                                         <  SRR4302547.2981075/94‑1 (MQ=60)
                                      CAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCG                                                        <  SRR4302547.4150495/94‑1 (MQ=60)
                                       AATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGA                                                       >  SRR4302547.1067621/1‑94 (MQ=60)
                                       AATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGA                                                       >  SRR4302547.6410702/1‑94 (MQ=60)
                                        ATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAA                                                      <  SRR4302547.3481237/94‑1 (MQ=60)
                                        ATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAA                                                      >  SRR4302547.2971533/1‑94 (MQ=60)
                                          AATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATG                                                    <  SRR4302547.5299731/94‑1 (MQ=60)
                                           ATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGG                                                   <  SRR4302547.1129069/94‑1 (MQ=60)
                                           ATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGG                                                   >  SRR4302547.2457208/1‑94 (MQ=60)
                                            TCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGC                                                  <  SRR4302547.1608530/94‑1 (MQ=60)
                                             CCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCT                                                 <  SRR4302547.3973364/94‑1 (MQ=60)
                                              CGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTT                                                >  SRR4302547.2636214/1‑94 (MQ=60)
                                              CGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTT                                                >  SRR4302547.2186844/1‑94 (MQ=60)
                                                  GAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATT                                            >  SRR4302547.4562040/1‑94 (MQ=60)
                                                   AATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTC                                           >  SRR4302547.872274/1‑94 (MQ=60)
                                                   AATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTC                                           >  SRR4302547.4695319/1‑94 (MQ=60)
                                                   AATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTC                                           >  SRR4302547.2508293/1‑94 (MQ=60)
                                                    ATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGCATGGCTTAATTCG                                          >  SRR4302547.6329577/1‑94 (MQ=60)
                                                    ATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCG                                          <  SRR4302547.1573794/94‑1 (MQ=60)
                                                    ATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCG                                          <  SRR4302547.2974738/94‑1 (MQ=60)
                                                       ATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAA                                       <  SRR4302547.220626/94‑1 (MQ=60)
                                                        TTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTACA                                      >  SRR4302547.6275586/1‑94 (MQ=60)
                                                         TATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAA                                     >  SRR4302547.6552661/1‑94 (MQ=60)
                                                           TGTCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACC                                   <  SRR4302547.638551/94‑1 (MQ=60)
                                                             TCCGTGCCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCC                                 <  SRR4302547.3102712/94‑1 (MQ=60)
                                                             TCCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCC                                 >  SRR4302547.4748526/1‑94 (MQ=60)
                                                              CCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCT                                <  SRR4302547.5546388/94‑1 (MQ=60)
                                                              CCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCT                                >  SRR4302547.202041/1‑94 (MQ=60)
                                                              CCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCT                                <  SRR4302547.3838927/94‑1 (MQ=60)
                                                              CCGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCT                                <  SRR4302547.3667682/94‑1 (MQ=60)
                                                               CGTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTG                               <  SRR4302547.3761866/94‑1 (MQ=60)
                                                                GTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACGCCGAATGGCTTAATTCGTAAAACCCCTGG                              <  SRR4302547.2503788/94‑1 (MQ=60)
                                                                GTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGG                              <  SRR4302547.3197113/94‑1 (MQ=60)
                                                                GTACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGG                              >  SRR4302547.214807/1‑94 (MQ=60)
                                                                 TACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGC                             >  SRR4302547.34832/1‑94 (MQ=60)
                                                                 TACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGC                             <  SRR4302547.5454920/94‑1 (MQ=60)
                                                                  ACCTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCT                            <  SRR4302547.3751386/94‑1 (MQ=60)
                                                                    CTTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTC                          <  SRR4302547.2917453/94‑1 (MQ=60)
                                                                     TTCTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCG                         >  SRR4302547.2178975/1‑94 (MQ=60)
                                                                       CTTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGC                       <  SRR4302547.3556772/94‑1 (MQ=60)
                                                                        TTCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCC                      >  SRR4302547.1715412/1‑94 (MQ=60)
                                                                         TCCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCC                     >  SRR4302547.4054015/1‑94 (MQ=60)
                                                                          CCGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCA                    >  SRR4302547.5239269/1‑94 (MQ=60)
                                                                           CGTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCAC                   >  SRR4302547.2521084/1‑94 (MQ=60)
                                                                            GTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACG                  <  SRR4302547.4278295/94‑1 (MQ=60)
                                                                            GTGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACG                  >  SRR4302547.2586217/1‑94 (MQ=60)
                                                                             TGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGT                 >  SRR4302547.2351263/1‑94 (MQ=60)
                                                                             TGGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGT                 >  SRR4302547.3387319/1‑94 (MQ=60)
                                                                              GGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTT                >  SRR4302547.3338640/1‑94 (MQ=60)
                                                                              GGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTT                >  SRR4302547.1303933/1‑94 (MQ=60)
                                                                              GGCGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTT                <  SRR4302547.5032683/94‑1 (MQ=60)
                                                                                CGTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCG              >  SRR4302547.5821567/1‑94 (MQ=60)
                                                                                 GTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGG             >  SRR4302547.668605/1‑94 (MQ=60)
                                                                                 GTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGG             <  SRR4302547.4610090/94‑1 (MQ=60)
                                                                                 GTTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGG             <  SRR4302547.6289447/94‑1 (MQ=60)
                                                                                  TTGATATTAGCCAGTTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGC            <  SRR4302547.5720143/94‑1 (MQ=60)
                                                                                  TTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGC            <  SRR4302547.1664378/94‑1 (MQ=60)
                                                                                  TTGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGC            >  SRR4302547.4634306/1‑94 (MQ=60)
                                                                                   TGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCA           <  SRR4302547.4726483/94‑1 (MQ=60)
                                                                                   TGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCA           >  SRR4302547.2744926/1‑94 (MQ=60)
                                                                                   TGATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCA           >  SRR4302547.5114539/1‑94 (MQ=60)
                                                                                    GATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCAC          <  SRR4302547.335068/94‑1 (MQ=60)
                                                                                     ATATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACT         <  SRR4302547.3655825/94‑1 (MQ=60)
                                                                                      TATTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTG        >  SRR4302547.1330089/1‑94 (MQ=60)
                                                                                        TTAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAA      >  SRR4302547.3716838/1‑94 (MQ=60)
                                                                                         TAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAA     <  SRR4302547.4316557/94‑1 (MQ=60)
                                                                                         TAGCCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAA     <  SRR4302547.4523311/94‑1 (MQ=60)
                                                                                            CCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTT  >  SRR4302547.3713296/1‑94 (MQ=60)
                                                                                            CCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTA  <  SRR4302547.6340819/94‑1 (MQ=60)
                                                                                            CCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTA  >  SRR4302547.5064853/1‑94 (MQ=60)
                                                                                            CCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTA  <  SRR4302547.5459009/94‑1 (MQ=60)
                                                                                            CCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTA  >  SRR4302547.2434494/1‑94 (MQ=60)
                                                                                            |                                                                                             
GGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTA  >  NC_000913/2803556‑2803741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑