Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,898,328 (A)9→8 intergenic (‑33/‑286) b2774 ← / → yqcE putative oxidoreductase/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,898,3200A.82.9% 757.8 / 196.6 247intergenic (‑25/‑294)b2774/yqcEputative oxidoreductase/putative transport protein
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base . (86/118);  total (105/142)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-02

Only 100 of 277 total aligned reads displayed.

ACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAA  >  NC_000913/2898228‑2898412
                                                                                            |                                                                                            
aCAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCa                                                                                              >  2:6353211/1‑93 (MQ=255)
 cAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaa                                                                                             <  2:1244978/93‑1 (MQ=255)
  aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaaa                                                                                            >  2:5701455/1‑93 (MQ=255)
         gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaaaaaaaaga                                                                                     >  1:6587975/1‑91 (MQ=255)
           tttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaaaaaaaagaaa                                                                                   <  2:5718980/93‑5 (MQ=255)
           tttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaaaaaaaagaaa                                                                                   <  1:6065720/93‑5 (MQ=255)
           tttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaaaaaaaagaaa                                                                                   <  1:4552641/93‑5 (MQ=255)
            ttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAaaagaaag                                                                                 >  2:655339/1‑93 (MQ=255)
            ttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAaaagaaag                                                                                 <  1:2327190/93‑1 (MQ=255)
             tACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAgg                                                                                <  1:1114179/93‑1 (MQ=255)
              aCCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGt                                                                               <  2:2969463/93‑1 (MQ=255)
              aCCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGt                                                                               <  2:2780729/93‑1 (MQ=255)
               ccTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTa                                                                              >  2:4717293/1‑93 (MQ=255)
               ccTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTa                                                                              <  1:1782849/93‑1 (MQ=255)
                 tttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTaa                                                                             <  2:323828/93‑1 (MQ=255)
                 tttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAAc                                                                            <  2:2880066/93‑1 (MQ=255)
                 tttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAAc                                                                            <  1:2813620/93‑1 (MQ=255)
                  ttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAAc                                                                            <  1:1011410/93‑1 (MQ=255)
                   tCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACtt                                                                          >  2:701057/1‑93 (MQ=255)
                    cAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTc                                                                         <  1:3128476/93‑1 (MQ=255)
                     aGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTcc                                                                        >  1:2446840/1‑93 (MQ=255)
                      gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTccc                                                                       >  1:2706315/1‑93 (MQ=255)
                       ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTcccc                                                                      <  2:4479488/93‑1 (MQ=255)
                        gagaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTccccc                                                                     <  1:212623/93‑1 (MQ=255)
                          gaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCcg                                                                    >  1:2308765/1‑93 (MQ=255)
                              aaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                               >  1:4748070/1‑93 (MQ=255)
                              aaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                               >  1:626283/1‑93 (MQ=255)
                              aaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                               <  2:5594624/93‑1 (MQ=255)
                               aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGagag                                                              <  2:2436376/93‑1 (MQ=255)
                               aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGagag                                                              >  1:5895748/1‑93 (MQ=255)
                                aTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                             <  2:4962182/93‑1 (MQ=255)
                                  ccATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGa                                                           <  2:6046137/93‑1 (MQ=255)
                                  ccATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGa                                                           <  2:225838/93‑1 (MQ=255)
                                   cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                          <  1:4248398/93‑1 (MQ=255)
                                   cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                          <  1:1992709/93‑1 (MQ=255)
                                   cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAA‑GCCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                          <  2:1574586/93‑1 (MQ=255)
                                     ttGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGt                                                        <  2:3967154/93‑1 (MQ=255)
                                      tGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGt                                                        <  2:1334455/93‑1 (MQ=255)
                                        agaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAc                                                     >  1:1678669/1‑93 (MQ=255)
                                         gaccGCTTTAAGAGATTGGATTGCCATAGTTAGTTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGAAACTTCCCCCGCGAGAGGGAAGTTAct                                                    >  1:5803581/4‑92 (MQ=255)
                                         gaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                    >  2:1150754/1‑93 (MQ=255)
                                         gaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                    >  2:1927785/1‑93 (MQ=255)
                                         gaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                    >  1:1515788/1‑93 (MQ=255)
                                           aCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCt                                                   <  2:5267146/93‑1 (MQ=255)
                                           aCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCtt                                                  >  2:5647064/1‑93 (MQ=255)
                                           aCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAAGTAACTTCCCCCGCGAGAGGGAAGTTACCtt                                                  >  2:6440615/1‑93 (MQ=255)
                                           aCGCAGTGAGAGATTCGATTGACATAGTTAGCTCCAAGTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCtt                                                  <  2:1691163/93‑1 (MQ=39)
                                            cGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtc                                                <  2:5990736/93‑1 (MQ=255)
                                             gCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCttt                                                 <  1:3655049/93‑1 (MQ=255)
                                             gCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtc                                                <  2:4394169/93‑1 (MQ=255)
                                              cATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtct                                               <  1:6315813/93‑1 (MQ=255)
                                               aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                              >  2:5804328/1‑93 (MQ=255)
                                                ttGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCa                                             >  1:3390987/1‑93 (MQ=255)
                                                 tGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCAt                                            <  2:3778808/93‑1 (MQ=255)
                                                   agagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTa                                          >  1:5726210/1‑93 (MQ=255)
                                                    gagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAt                                         <  1:863607/93‑1 (MQ=255)
                                                      gaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtct                                       >  1:2508047/1‑93 (MQ=255)
                                                       aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtct                                       <  1:5380305/93‑1 (MQ=255)
                                                       aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtct                                       <  1:729765/93‑1 (MQ=255)
                                                       aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                      <  1:5924981/93‑1 (MQ=255)
                                                       aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                      <  2:3450373/93‑1 (MQ=255)
                                                       aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                      >  2:4779074/1‑93 (MQ=255)
                                                       aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTACCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                      <  2:4584784/93‑1 (MQ=255)
                                                        ttCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                      <  2:6243701/93‑1 (MQ=255)
                                                        ttCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATctct                                     <  2:6140350/93‑1 (MQ=255)
                                                        ttCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATctct                                     >  2:3694864/1‑93 (MQ=255)
                                                        ttCGATTCACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                      <  2:3165010/93‑1 (MQ=255)
                                                          cGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTg                                    <  2:2524703/93‑1 (MQ=255)
                                                          cGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                   <  1:4833868/93‑1 (MQ=255)
                                                            aTTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                 >  1:4445412/1‑93 (MQ=255)
                                                            aTTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                 >  2:351281/1‑93 (MQ=255)
                                                              tGACATAGTTAGCTCCAATCTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAAc                               >  1:6467360/1‑93 (MQ=255)
                                                               gACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACt                              <  1:5247283/93‑1 (MQ=255)
                                                                aCATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTg                             <  1:4384110/93‑1 (MQ=255)
                                                                 cATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                            >  1:1924194/1‑93 (MQ=255)
                                                                  aTAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGga                           >  1:3869158/1‑93 (MQ=255)
                                                                    aGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGgaga                         <  1:4656539/93‑1 (MQ=255)
                                                                    aGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGgaga                         <  2:5404436/93‑1 (MQ=255)
                                                                     gTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGgaga                         >  2:5456586/1‑93 (MQ=255)
                                                                     gTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                        >  2:391443/1‑93 (MQ=255)
                                                                     gTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                        <  2:5887570/93‑1 (MQ=255)
                                                                         gCTCCAATTTCGGTAAGGC‑AAAAAAAAGAAGGGTAACTGCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtt                    <  2:3012834/93‑1 (MQ=255)
                                                                         gCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtt                    >  2:5363873/1‑93 (MQ=255)
                                                                              aaTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                <  2:4203552/93‑1 (MQ=255)
                                                                              aaTTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg               <  2:2632953/93‑1 (MQ=255)
                                                                               aTTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc              >  2:1813302/1‑93 (MQ=255)
                                                                               aTTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc              >  1:953677/1‑93 (MQ=255)
                                                                                    cGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAg         <  1:4342050/93‑1 (MQ=255)
                                                                                     gTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg        <  2:5146351/93‑1 (MQ=255)
                                                                                     gTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg        >  2:6317788/1‑93 (MQ=255)
                                                                                     gTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg        <  1:2239793/93‑1 (MQ=255)
                                                                                      tAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg       >  2:4026309/1‑93 (MQ=255)
                                                                                      tAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg       <  1:1245585/93‑1 (MQ=255)
                                                                                      tAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg       >  1:5651616/1‑93 (MQ=255)
                                                                                      tAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg       >  1:347914/1‑93 (MQ=255)
                                                                                       aaGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg       >  2:1889927/1‑93 (MQ=255)
                                                                                       aaGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc      >  1:4994037/1‑93 (MQ=255)
                                                                                         ggCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCa     >  2:848716/1‑93 (MQ=255)
                                                                                           cAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATa   >  2:2179100/1‑93 (MQ=255)
                                                                                            aaaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa  <  1:5160181/93‑1 (MQ=255)
                                                                                            |                                                                                            
ACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAA  >  NC_000913/2898228‑2898412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGT  >  NC_000913/2898235‑2898422
                                                                                             |                                                                                              
CGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAG                                                                                                <  SRR4302547.6728248/94‑1 (MQ=60)
  GTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAA                                                                                             <  SRR4302547.2522330/94‑1 (MQ=60)
    TTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAG                                                                                           >  SRR4302547.2274307/1‑94 (MQ=60)
    TTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAG                                                                                           <  SRR4302547.4756798/94‑1 (MQ=60)
    TTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAG                                                                                           <  SRR4302547.4693642/94‑1 (MQ=60)
    TTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAG                                                                                           <  SRR4302547.6191537/94‑1 (MQ=60)
    TTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAG                                                                                           <  SRR4302547.4646815/94‑1 (MQ=60)
     TTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAG                                                                                           <  SRR4302547.1794369/94‑1 (MQ=60)
     TTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGG                                                                                          <  SRR4302547.2374357/94‑1 (MQ=60)
     TTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGG                                                                                          <  SRR4302547.4187944/94‑1 (MQ=60)
      TACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGT                                                                                         <  SRR4302547.1136614/94‑1 (MQ=60)
      TACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGT                                                                                         <  SRR4302547.2125949/94‑1 (MQ=60)
       ACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTA                                                                                        >  SRR4302547.1034902/1‑94 (MQ=60)
        CCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTA                                                                                        <  SRR4302547.561378/94‑1 (MQ=60)
        CCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAA                                                                                       <  SRR4302547.1818166/94‑1 (MQ=60)
         CTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAAC                                                                                      <  SRR4302547.91954/94‑1 (MQ=60)
          TTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACT                                                                                     <  SRR4302547.2869909/94‑1 (MQ=60)
           TTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACT                                                                                     <  SRR4302547.1031173/94‑1 (MQ=60)
            TCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTC                                                                                   >  SRR4302547.4657262/1‑94 (MQ=60)
             CAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCC                                                                                  <  SRR4302547.3190556/94‑1 (MQ=60)
             CAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCC                                                                                  >  SRR4302547.2496302/1‑94 (MQ=60)
              AGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCC                                                                                 >  SRR4302547.2760661/1‑94 (MQ=60)
               GGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCA                                                                                <  SRR4302547.4443908/94‑1 (MQ=60)
               GGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGAATGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCC                                                                                >  SRR4302547.5320468/1‑94 (MQ=60)
                GGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCC                                                                               >  SRR4302547.2743445/1‑94 (MQ=60)
                 GAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCC                                                                               <  SRR4302547.2023476/94‑1 (MQ=60)
                 GAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCC                                                                               <  SRR4302547.771192/94‑1 (MQ=60)
                 GAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCG                                                                              <  SRR4302547.3477833/94‑1 (MQ=60)
                 GAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCG                                                                              <  SRR4302547.3410502/94‑1 (MQ=60)
                 GAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCG                                                                              <  SRR4302547.216633/94‑1 (MQ=60)
                  AGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCG                                                                              >  SRR4302547.2355582/1‑94 (MQ=60)
                  AGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCG                                                                              <  SRR4302547.4485482/94‑1 (MQ=60)
                  AGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGC                                                                             <  SRR4302547.1253254/94‑1 (MQ=60)
                  AGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGC                                                                             <  SRR4302547.2482880/94‑1 (MQ=60)
                     AAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGA                                                                           >  SRR4302547.3117969/1‑94 (MQ=60)
                      AAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGA                                                                         >  SRR4302547.4846457/1‑94 (MQ=60)
                      AAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGA                                                                         >  SRR4302547.637848/1‑94 (MQ=60)
                       AAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAG                                                                        >  SRR4302547.6017829/1‑94 (MQ=60)
                       AAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAG                                                                        >  SRR4302547.6757142/1‑94 (MQ=60)
                       AAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGACAATTCCCCCGCGAGAG                                                                        >  SRR4302547.2357174/1‑94 (MQ=60)
                         ATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGG                                                                      <  SRR4302547.1551/94‑1 (MQ=60)
                          TCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGA                                                                     <  SRR4302547.1853406/94‑1 (MQ=60)
                           CCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAA                                                                    <  SRR4302547.2801879/94‑1 (MQ=60)
                           CCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAA                                                                    >  SRR4302547.2425731/1‑94 (MQ=60)
                            CATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAG                                                                   <  SRR4302547.2538269/94‑1 (MQ=60)
                            CATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAG                                                                   <  SRR4302547.2032333/94‑1 (MQ=60)
                            CATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAG                                                                   <  SRR4302547.4997033/94‑1 (MQ=60)
                            CATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAG                                                                   <  SRR4302547.4335304/94‑1 (MQ=60)
                              TTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTT                                                                 >  SRR4302547.5899396/1‑94 (MQ=60)
                               TGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTA                                                                >  SRR4302547.625580/1‑94 (MQ=60)
                               TGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTA                                                                <  SRR4302547.3924354/94‑1 (MQ=60)
                                GAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAC                                                               >  SRR4302547.1711728/1‑94 (MQ=60)
                                 AGACCGCTTTAAGAGATTGGATTGCCATAGTTAGTTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGAAACTTCCCCCGCGAGAGGGAAGTTACT                                                              >  SRR4302547.5923652/1‑94 (MQ=60)
                                 AGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTTCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACC                                                              >  SRR4302547.1151739/1‑94 (MQ=60)
                                 AGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACC                                                              >  SRR4302547.1545989/1‑94 (MQ=60)
                                  GAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCT                                                             >  SRR4302547.4590419/1‑94 (MQ=60)
                                  GAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCT                                                             <  SRR4302547.146401/94‑1 (MQ=60)
                                  GAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCT                                                             >  SRR4302547.4853884/1‑94 (MQ=60)
                                  GAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTT                                                            >  SRR4302547.2298483/1‑94 (MQ=60)
                                    ACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTT                                                            >  SRR4302547.2957849/1‑94 (MQ=60)
                                    ACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTT                                                            >  SRR4302547.3580931/1‑94 (MQ=60)
                                    ACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTT                                                           <  SRR4302547.6643843/94‑1 (MQ=60)
                                      GCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTC                                                          <  SRR4302547.3729616/94‑1 (MQ=60)
                                       CATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTC                                                        <  SRR4302547.6447143/94‑1 (MQ=60)
                                       CATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTC                                                        <  SRR4302547.1993431/94‑1 (MQ=60)
                                        ATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCA                                                       <  SRR4302547.4897186/94‑1 (MQ=60)
                                        ATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCA                                                       >  SRR4302547.4380872/1‑94 (MQ=60)
                                        ATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCA                                                       >  SRR4302547.3459969/1‑94 (MQ=60)
                                        ATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGCTACCTTTCTCA                                                       >  SRR4302547.4573046/1‑94 (MQ=60)
                                         TTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCAT                                                      >  SRR4302547.6045689/1‑94 (MQ=60)
                                          TGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATT                                                     >  SRR4302547.1392955/1‑94 (MQ=60)
                                           GAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTA                                                    <  SRR4302547.1638759/94‑1 (MQ=60)
                                           GAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTA                                                    >  SRR4302547.5844503/1‑94 (MQ=60)
                                            AGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAT                                                   <  SRR4302547.6267802/94‑1 (MQ=60)
                                             GAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATC                                                  >  SRR4302547.92267/1‑94 (MQ=60)
                                             GAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATC                                                  <  SRR4302547.880125/94‑1 (MQ=60)
                                              AGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCT                                                 >  SRR4302547.2558755/1‑94 (MQ=60)
                                               AATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTC                                                <  SRR4302547.1837553/94‑1 (MQ=60)
                                                ATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTC                                                <  SRR4302547.743381/94‑1 (MQ=60)
                                                ATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTC                                                <  SRR4302547.5491388/94‑1 (MQ=60)
                                                ATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCT                                               <  SRR4302547.6047794/94‑1 (MQ=60)
                                                 TTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTG                                              <  SRR4302547.5240244/94‑1 (MQ=60)
                                                 TTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTG                                              <  SRR4302547.5783908/94‑1 (MQ=60)
                                                 TTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTG                                              <  SRR4302547.2070019/94‑1 (MQ=60)
                                                  TCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGG                                             >  SRR4302547.3825108/1‑94 (MQ=60)
                                                   CGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGT                                            <  SRR4302547.4934074/94‑1 (MQ=60)
                                                    GATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTA                                           >  SRR4302547.4537355/1‑94 (MQ=60)
                                                      TTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAAC                                         <  SRR4302547.5366943/94‑1 (MQ=60)
                                                      TTGACATAGTTAGCTCCAATCTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAAC                                         >  SRR4302547.6602037/1‑94 (MQ=60)
                                                       TGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACT                                        <  SRR4302547.4507614/94‑1 (MQ=60)
                                                       TGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACT                                        <  SRR4302547.5814679/94‑1 (MQ=60)
                                                        GACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTG                                       <  SRR4302547.5355299/94‑1 (MQ=60)
                                                         ACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGG                                      <  SRR4302547.4474296/94‑1 (MQ=60)
                                                         ACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGG                                      >  SRR4302547.1962311/1‑94 (MQ=60)
                                                         ACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGG                                      >  SRR4302547.2305964/1‑94 (MQ=60)
                                                          CATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGA                                     >  SRR4302547.3947925/1‑94 (MQ=60)
                                                             AGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAT                                  <  SRR4302547.4752979/94‑1 (MQ=60)
                                                                 AGCTCCAATTTCCGTAAAGCAAAAAAAAAGAAAGGAAACTTACCCCGCCGGAGGGTAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAT                               >  SRR4302547.4232104/1‑94 (MQ=60)
                                                                  GCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTT                             >  SRR4302547.442282/1‑94 (MQ=60)
                                                                   ctcaaattcccgaaggaaAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTT                             <  SRR4302547.5863734/76‑1 (MQ=60)
                                                                    TGCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTT                           >  SRR4302547.6513940/1‑94 (MQ=60)
                                                                    TCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTT                           <  SRR4302547.6409365/94‑1 (MQ=60)
                                                                      CAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAG                         <  SRR4302547.5462838/94‑1 (MQ=60)
                                                                       AATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGC                        >  SRR4302547.972205/1‑94 (MQ=60)
                                                                             CGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGG                  <  SRR4302547.5923741/94‑1 (MQ=60)
                                                                             CGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGG                  <  SRR4302547.6009916/94‑1 (MQ=60)
                                                                             CGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGG                  <  SRR4302547.4431328/94‑1 (MQ=60)
                                                                             CGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGG                  <  SRR4302547.3451153/94‑1 (MQ=60)
                                                                              GTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGG                  <  SRR4302547.2776773/94‑1 (MQ=60)
                                                                              GTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGG                 >  SRR4302547.5768289/1‑94 (MQ=60)
                                                                              GTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGG                 <  SRR4302547.2284961/94‑1 (MQ=60)
                                                                              GTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGG                 >  SRR4302547.354377/1‑94 (MQ=60)
                                                                               TAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGC                >  SRR4302547.5097291/1‑94 (MQ=60)
                                                                               TAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGC                <  SRR4302547.1270724/94‑1 (MQ=60)
                                                                                 AGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCA               <  SRR4302547.3641454/94‑1 (MQ=60)
                                                                                 AGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCA               >  SRR4302547.5671114/1‑94 (MQ=60)
                                                                                 AGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAT              >  SRR4302547.3488184/1‑94 (MQ=60)
                                                                                 AGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAT              <  SRR4302547.4600727/94‑1 (MQ=60)
                                                                                 AGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAT              <  SRR4302547.6483857/94‑1 (MQ=60)
                                                                                   GCAAAAAAAAAGAAAGGCAACTTCCCCCGCGAGAGGGAAGTGACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATA             <  SRR4302547.6711173/94‑1 (MQ=60)
                                                                                    gcAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAA            <  SRR4302547.2100023/92‑1 (MQ=60)
                                                                                    gcAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAA            <  SRR4302547.6024376/92‑1 (MQ=60)
                                                                                     CAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAAC           <  SRR4302547.5192565/94‑1 (MQ=60)
                                                                                     AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAAC           <  SRR4302547.5266432/94‑1 (MQ=60)
                                                                                      AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACT          <  SRR4302547.1756379/94‑1 (MQ=60)
                                                                                       AAAAAAAGAAAGGTACCTTCCCCCGCGAGAGGGAAGTTCCCTTTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTG         <  SRR4302547.4215506/94‑1 (MQ=60)
                                                                                       AAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTG         >  SRR4302547.6714141/1‑94 (MQ=60)
                                                                                          AAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTA      >  SRR4302547.6371305/1‑94 (MQ=60)
                                                                                            AAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAT    >  SRR4302547.3247276/1‑94 (MQ=60)
                                                                                            AAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAT    <  SRR4302547.6460295/94‑1 (MQ=60)
                                                                                            AAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAT    >  SRR4302547.840721/1‑94 (MQ=60)
                                                                                             AGAAAGGTACCTCCCCCCGCGAGAGGGAAGTTACC‑TTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGT  <  SRR4302547.2852044/94‑1 (MQ=60)
                                                                                             AGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGGATAACTGCTAATG   >  SRR4302547.4993138/1‑94 (MQ=60)
                                                                                             AGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATG   >  SRR4302547.3511014/1‑94 (MQ=60)
                                                                                             AGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATG   <  SRR4302547.1366393/94‑1 (MQ=60)
                                                                                             |                                                                                              
CGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGT  >  NC_000913/2898235‑2898422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑