Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,905,601 A→G A121A (GCT→GCC)  eno ← enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,905,6010AG84.5% 606.6 / 102.5 246A121A (GCT→GCC) enoenolase
Reads supporting (aligned to +/- strand):  ref base A (22/16);  new base G (96/112);  total (118/128)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 259 total aligned reads displayed.

CATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTG  >  NC_000913/2905511‑2905691
                                                                                          |                                                                                          
 atcatcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCt                                                                                         >  2:5683135/1‑93 (MQ=255)
 atcatcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCt                                                                                         <  2:3489920/93‑1 (MQ=255)
   catcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTGCAGCGGCATACCTTTAGCAGCTGCAGCGGCttt                                                                                       >  2:6480931/1‑93 (MQ=255)
    atcGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTg                                                                                      <  1:3135994/93‑1 (MQ=255)
         gaCCGGCATAGGGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGtt                                                                                 <  2:1524164/92‑1 (MQ=255)
          aCCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTa                                                                                >  2:3864328/1‑93 (MQ=255)
          aCCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTa                                                                                <  1:6229853/93‑1 (MQ=255)
           ccGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAg                                                                               >  1:6405198/1‑93 (MQ=255)
            cGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGc                                                                              >  2:3215663/1‑93 (MQ=255)
                aTAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCaga                                                                          <  2:5727623/93‑1 (MQ=255)
                aTAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCaga                                                                          <  1:1263884/93‑1 (MQ=255)
                 tAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCagag                                                                         >  2:786869/1‑93 (MQ=255)
                   gagTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGAt                                                                       >  1:2955342/1‑93 (MQ=255)
                        tttGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGc                                                                  <  2:663291/93‑1 (MQ=255)
                        tttGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCAGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGc                                                                  <  2:2817045/93‑1 (MQ=255)
                           gCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAg                                                               <  2:1389078/93‑1 (MQ=255)
                           gCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAg                                                               >  2:1725905/1‑93 (MQ=255)
                            cccGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAgg                                                              >  2:3128798/1‑93 (MQ=255)
                             ccGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGa                                                             >  2:6618638/1‑93 (MQ=255)
                             ccGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGa                                                             <  1:4482847/93‑1 (MQ=255)
                              cGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                                                            <  1:2725487/93‑1 (MQ=255)
                              cGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                                                            <  1:1152292/93‑1 (MQ=255)
                              cGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                                                            >  2:4419743/1‑93 (MQ=255)
                               ggAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAtt                                                           >  2:3601075/1‑93 (MQ=255)
                               ggAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAtt                                                           <  1:4703911/93‑1 (MQ=255)
                               ggAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGAtt                                                           >  1:1512453/1‑93 (MQ=255)
                                gAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTg                                                          >  1:1028875/1‑93 (MQ=255)
                                gAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTg                                                          <  2:5427072/93‑1 (MQ=255)
                                gAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTg                                                          >  2:5536771/1‑93 (MQ=255)
                                  gTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCg                                                        >  1:1352647/1‑93 (MQ=255)
                                   tACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGt                                                       <  2:685953/93‑1 (MQ=255)
                                    aCCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGtt                                                      <  1:3938521/93‑1 (MQ=255)
                                      cGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcg                                                    <  2:3985169/93‑1 (MQ=255)
                                      cGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcg                                                    <  2:5577797/93‑1 (MQ=255)
                                       gttcagttcaGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgc                                                   >  1:3654852/1‑93 (MQ=255)
                                       gttcagttcaGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgc                                                   >  2:6039921/1‑93 (MQ=255)
                                       gttcagttcaGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgc                                                   >  2:693919/1‑93 (MQ=255)
                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  >  2:5209119/1‑93 (MQ=255)
                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  >  2:204082/1‑93 (MQ=255)
                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  >  2:2161059/1‑93 (MQ=255)
                                        ttcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                                                  <  2:3820319/93‑1 (MQ=255)
                                         tcagttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCgcgc                                                 >  1:2323170/1‑93 (MQ=255)
                                           agttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCg                                               >  2:5807016/1‑93 (MQ=255)
                                            gttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGa                                              >  1:2579922/1‑93 (MQ=255)
                                            gttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGa                                              >  2:1699281/1‑93 (MQ=255)
                                             ttcagCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGaa                                             <  2:6166436/93‑1 (MQ=255)
                                                 gCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTg                                         <  2:2695526/93‑1 (MQ=255)
                                                 gCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTg                                         <  1:2384752/93‑1 (MQ=255)
                                                 gCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGAGTTCGCGCCGAATTTg                                         >  2:559716/1‑93 (MQ=255)
                                                  cGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTgg                                        >  2:1887674/1‑93 (MQ=255)
                                                    aTGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAt                                      <  1:1678797/93‑1 (MQ=255)
                                                    aTGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAt                                      <  1:4093863/93‑1 (MQ=255)
                                                    aTGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGAGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAt                                      >  2:6554434/1‑93 (MQ=255)
                                                     tgtgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTTGAtt                                     <  1:5902860/93‑1 (MQ=255)
                                                     tgtgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAtt                                     >  2:2346535/1‑93 (MQ=255)
                                                      gtgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAttt                                    >  1:4899176/1‑93 (MQ=255)
                                                       tgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTg                                   >  2:5981054/1‑93 (MQ=255)
                                                       tgCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTg                                   >  1:1134671/1‑93 (MQ=255)
                                                        gCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGt                                  <  1:3501384/93‑1 (MQ=255)
                                                        gCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGt                                  <  1:1886347/93‑1 (MQ=255)
                                                         cTCGTACAGCGTCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 <  1:4301924/93‑1 (MQ=255)
                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 <  2:5263080/93‑1 (MQ=255)
                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 >  2:2673100/1‑93 (MQ=255)
                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 >  1:5366537/1‑93 (MQ=255)
                                                         cTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtt                                 <  1:1604602/93‑1 (MQ=255)
                                                          tCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGttt                                >  1:6556187/1‑93 (MQ=255)
                                                          tCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGttt                                >  1:5729162/1‑93 (MQ=255)
                                                           cGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtttt                               <  1:3275046/93‑1 (MQ=255)
                                                            gTACAGCGTCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGtttta                              >  1:6445428/1‑92 (MQ=255)
                                                            gTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTc                              >  1:1749466/1‑93 (MQ=255)
                                                             tACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCg                             <  1:2045368/93‑1 (MQ=255)
                                                             tACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCg                             <  1:891946/93‑1 (MQ=255)
                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           <  2:4879086/93‑1 (MQ=255)
                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           <  1:4372873/93‑1 (MQ=255)
                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           >  2:3853574/1‑93 (MQ=255)
                                                               cAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGt                           >  1:5549258/1‑93 (MQ=255)
                                                                aGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTg                          >  1:6151743/1‑93 (MQ=255)
                                                                aGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTg                          >  2:1428168/1‑93 (MQ=255)
                                                                 gCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGc                         <  1:5279216/93‑1 (MQ=255)
                                                                  cGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGcc                        >  2:4947097/1‑93 (MQ=255)
                                                                  cGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGcc                        <  1:3974411/93‑1 (MQ=255)
                                                                   ggCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCg                       <  2:3314513/93‑1 (MQ=255)
                                                                   ggCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCg                       >  2:1897029/1‑93 (MQ=255)
                                                                   ggCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCg                       <  1:955608/93‑1 (MQ=255)
                                                                    gCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGt                      >  2:3781888/1‑93 (MQ=255)
                                                                       tACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCa                   <  2:3892773/93‑1 (MQ=255)
                                                                        aCCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAg                  <  1:1304107/93‑1 (MQ=255)
                                                                          cTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGt                <  2:286418/93‑1 (MQ=255)
                                                                          cTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGt                >  1:2221100/1‑93 (MQ=255)
                                                                          cTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGt                <  1:3706840/93‑1 (MQ=255)
                                                                            ttAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCg              <  2:4108261/93‑1 (MQ=255)
                                                                             tAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGa             >  1:3302643/1‑93 (MQ=255)
                                                                             tAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGa             <  2:4292202/93‑1 (MQ=255)
                                                                             tAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGa             <  1:3031130/93‑1 (MQ=255)
                                                                              agcagcTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGAt            >  2:5089603/1‑93 (MQ=255)
                                                                                cagcTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCa          >  2:2665617/1‑93 (MQ=255)
                                                                                cagcTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCa          >  1:2931662/1‑93 (MQ=255)
                                                                                 agcTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCAt         >  1:5570060/1‑93 (MQ=255)
                                                                                    tGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGAt      >  2:4598561/1‑93 (MQ=255)
                                                                                     gcagcgGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATc     <  1:6307990/93‑1 (MQ=255)
                                                                                          |                                                                                          
CATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTG  >  NC_000913/2905511‑2905691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTGTCA  >  NC_000913/2905508‑2905694
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GTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCG                                                                                               <  SRR4302547.5932618/94‑1 (MQ=60)
 TTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGG                                                                                              >  SRR4302547.1945785/1‑94 (MQ=60)
 TTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGG                                                                                              <  SRR4302547.2527032/94‑1 (MQ=60)
 TTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGG                                                                                              <  SRR4302547.2826269/94‑1 (MQ=60)
   CATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCT                                                                                            <  SRR4302547.11054/94‑1 (MQ=60)
    ATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTTCAGCGGCATACCTTTAGCAGCTTCAGCGGCTT                                                                                           <  SRR4302547.6623012/94‑1 (MQ=60)
    ATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTT                                                                                           >  SRR4302547.1308733/1‑94 (MQ=60)
     TCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTT                                                                                          <  SRR4302547.514040/94‑1 (MQ=60)
      CATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGGACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTG                                                                                         <  SRR4302547.2173315/94‑1 (MQ=60)
       ATCGGAGCCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCCCGTACAGCGGCATGCCTTTAGCAGCTGCAGCGGCTTTGG                                                                                        <  SRR4302547.1601437/94‑1 (MQ=60)
       ATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGG                                                                                        <  SRR4302547.3198266/94‑1 (MQ=60)
        TCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATAGCTTTAGCAGCTGCAGCGGCTTTGGC                                                                                       >  SRR4302547.5897954/1‑94 (MQ=60)
        TCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGC                                                                                       <  SRR4302547.4662058/94‑1 (MQ=60)
           GAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTT                                                                                    >  SRR4302547.252115/1‑94 (MQ=60)
            AACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTA                                                                                   >  SRR4302547.439545/1‑94 (MQ=60)
            AACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTA                                                                                   >  SRR4302547.3456454/1‑94 (MQ=60)
             ACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAG                                                                                  <  SRR4302547.6359254/94‑1 (MQ=60)
             ACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAG                                                                                  >  SRR4302547.6538784/1‑94 (MQ=60)
                  CATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGA                                                                             >  SRR4302547.2411399/1‑94 (MQ=60)
                  CATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGA                                                                             <  SRR4302547.2361785/94‑1 (MQ=60)
                  CATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGA                                                                             <  SRR4302547.3673198/94‑1 (MQ=60)
                   ATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAG                                                                            >  SRR4302547.4105596/1‑94 (MQ=60)
                   ATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAG                                                                            <  SRR4302547.1289337/94‑1 (MQ=60)
                   ATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAG                                                                            >  SRR4302547.2926753/1‑94 (MQ=60)
                     AGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGAT                                                                          <  SRR4302547.3203449/94‑1 (MQ=60)
                     AGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGAT                                                                          >  SRR4302547.4309878/1‑94 (MQ=60)
                     AGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGAT                                                                          >  SRR4302547.3014167/1‑94 (MQ=60)
                      GAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATA                                                                         >  SRR4302547.6556255/1‑94 (MQ=60)
                         TATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAG                                                                      >  SRR4302547.6437261/1‑94 (MQ=60)
                          ATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGC                                                                     <  SRR4302547.4529953/94‑1 (MQ=60)
                              GCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGGTAGCCAGAGATACAGCCAGG                                                                 <  SRR4302547.6623018/94‑1 (MQ=60)
                                CCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGAT                                                               <  SRR4302547.4575459/94‑1 (MQ=60)
                                 CGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATT                                                              <  SRR4302547.1772010/94‑1 (MQ=60)
                                 CGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATT                                                              <  SRR4302547.1175618/94‑1 (MQ=60)
                                 CGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATT                                                              <  SRR4302547.3280640/94‑1 (MQ=60)
                                 CGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATT                                                              <  SRR4302547.2780208/94‑1 (MQ=60)
                                 CGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATT                                                              >  SRR4302547.1542594/1‑94 (MQ=60)
                                  GGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTG                                                             <  SRR4302547.774599/94‑1 (MQ=60)
                                  GGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTG                                                             <  SRR4302547.4801326/94‑1 (MQ=60)
                                  GGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTG                                                             >  SRR4302547.1049084/1‑94 (MQ=60)
                                  GGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGGTAGCCAGAGATACAGCCAGGATTG                                                             >  SRR4302547.6708512/1‑94 (MQ=60)
                                    AGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCG                                                           >  SRR4302547.1379804/1‑94 (MQ=60)
                                       ACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTC                                                        <  SRR4302547.4018601/94‑1 (MQ=60)
                                        CCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCG                                                       >  SRR4302547.5320635/1‑94 (MQ=60)
                                         CGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGC                                                      >  SRR4302547.3729410/1‑94 (MQ=60)
                                          GTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCG                                                     >  SRR4302547.3738419/1‑94 (MQ=60)
                                           TTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGC                                                    >  SRR4302547.2370260/1‑94 (MQ=60)
                                            TCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCC                                                   <  SRR4302547.4373014/94‑1 (MQ=60)
                                              AGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGA                                                 >  SRR4302547.2631992/1‑94 (MQ=60)
                                                TTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAAT                                               <  SRR4302547.4943463/94‑1 (MQ=60)
                                                  CAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTT                                             <  SRR4302547.1409863/94‑1 (MQ=60)
                                                  CAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTT                                             >  SRR4302547.6417371/1‑94 (MQ=60)
                                                   AGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTG                                            <  SRR4302547.5931766/94‑1 (MQ=60)
                                                    GCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGG                                           <  SRR4302547.2433063/94‑1 (MQ=60)
                                                    GCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGG                                           <  SRR4302547.2216555/94‑1 (MQ=60)
                                                     CGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGA                                          >  SRR4302547.1166902/1‑94 (MQ=60)
                                                      GATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAT                                         >  SRR4302547.6448373/1‑94 (MQ=60)
                                                      GATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGAT                                         <  SRR4302547.1183192/94‑1 (MQ=60)
                                                       ATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATT                                        <  SRR4302547.1711857/94‑1 (MQ=60)
                                                       ATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATT                                        <  SRR4302547.4177110/94‑1 (MQ=60)
                                                       ATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATT                                        >  SRR4302547.5494203/1‑94 (MQ=60)
                                                        TGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTTGATTT                                       <  SRR4302547.6025103/94‑1 (MQ=60)
                                                        TGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTT                                       <  SRR4302547.3024618/94‑1 (MQ=60)
                                                        TGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTT                                       >  SRR4302547.5000626/1‑94 (MQ=60)
                                                         GTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTG                                      <  SRR4302547.1289993/94‑1 (MQ=60)
                                                         GTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTG                                      >  SRR4302547.1157589/1‑94 (MQ=60)
                                                           GCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTT                                    >  SRR4302547.5477274/1‑94 (MQ=60)
                                                           GCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTT                                    <  SRR4302547.1923618/94‑1 (MQ=60)
                                                           GCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTT                                    <  SRR4302547.3572836/94‑1 (MQ=60)
                                                            CTCGTACAGCGTCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTT                                   <  SRR4302547.4390405/94‑1 (MQ=60)
                                                            CTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTT                                   >  SRR4302547.6692706/1‑94 (MQ=60)
                                                            CTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTT                                   >  SRR4302547.5847517/1‑94 (MQ=60)
                                                            CTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTT                                   <  SRR4302547.5679710/94‑1 (MQ=60)
                                                            CTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTT                                   <  SRR4302547.2093127/94‑1 (MQ=60)
                                                            CTCGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTT                                   <  SRR4302547.1384392/94‑1 (MQ=60)
                                                            CTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTT                                   <  SRR4302547.1636429/94‑1 (MQ=60)
                                                              CGTACAGCGTCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTA                                 >  SRR4302547.6579726/1‑94 (MQ=60)
                                                              CGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTC                                 <  SRR4302547.3340662/94‑1 (MQ=60)
                                                              CGTACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTC                                 >  SRR4302547.1783994/1‑94 (MQ=60)
                                                               GTACAGCAGCATCCGTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCG                                <  SRR4302547.2746347/94‑1 (MQ=60)
                                                                TACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGG                               <  SRR4302547.2086167/94‑1 (MQ=60)
                                                                TACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGG                               <  SRR4302547.909097/94‑1 (MQ=60)
                                                                 ACAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGT                              >  SRR4302547.5663759/1‑94 (MQ=60)
                                                                  CAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTG                             <  SRR4302547.1767650/94‑1 (MQ=60)
                                                                  CAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTG                             <  SRR4302547.4462730/94‑1 (MQ=60)
                                                                  CAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTG                             <  SRR4302547.4470981/94‑1 (MQ=60)
                                                                  CAGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTG                             >  SRR4302547.6279386/1‑94 (MQ=60)
                                                                   AGCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGC                            >  SRR4302547.5721302/1‑94 (MQ=60)
                                                                    GCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCC                           <  SRR4302547.5388112/94‑1 (MQ=60)
                                                                    GCGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCC                           <  SRR4302547.3866609/94‑1 (MQ=60)
                                                                     CGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGGATTTGGATTTGTTTTCGGTGCCT                          >  SRR4302547.1628668/1‑94 (MQ=60)
                                                                     CGGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCG                          <  SRR4302547.4055232/94‑1 (MQ=60)
                                                                     CGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCG                          >  SRR4302547.5834626/1‑94 (MQ=60)
                                                                      GGCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGT                         <  SRR4302547.974173/94‑1 (MQ=60)
                                                                       GCATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTC                        >  SRR4302547.1965609/1‑94 (MQ=60)
                                                                        CATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCC                       >  SRR4302547.3059992/1‑94 (MQ=60)
                                                                        CATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCC                       >  SRR4302547.2662751/1‑94 (MQ=60)
                                                                        CATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCC                       >  SRR4302547.199960/1‑94 (MQ=60)
                                                                         ATACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCA                      <  SRR4302547.402917/94‑1 (MQ=60)
                                                                         ATACCTTTAGCAGCTGCAGCGGCTTTAGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCA                      <  SRR4302547.5468654/94‑1 (MQ=60)
                                                                          TACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAG                     >  SRR4302547.953902/1‑94 (MQ=60)
                                                                           TCCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGG                    <  SRR4302547.3448064/94‑1 (MQ=60)
                                                                           ACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGG                    >  SRR4302547.6462502/1‑94 (MQ=60)
                                                                           ACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGG                    <  SRR4302547.6134229/94‑1 (MQ=60)
                                                                           ACCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGG                    <  SRR4302547.1330343/94‑1 (MQ=60)
                                                                            CCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGT                   >  SRR4302547.3976062/1‑94 (MQ=60)
                                                                            CCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGT                   >  SRR4302547.2265847/1‑94 (MQ=60)
                                                                            CCTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGT                   <  SRR4302547.3106461/94‑1 (MQ=60)
                                                                            CCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGT                   >  SRR4302547.2940374/1‑94 (MQ=60)
                                                                             CTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTC                  <  SRR4302547.3782513/94‑1 (MQ=60)
                                                                             CTTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTC                  <  SRR4302547.3342647/94‑1 (MQ=60)
                                                                              TTTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCG                 <  SRR4302547.239176/94‑1 (MQ=60)
                                                                               TTAGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGA                >  SRR4302547.3369135/1‑94 (MQ=60)
                                                                                TAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGAT               <  SRR4302547.3091408/94‑1 (MQ=60)
                                                                                 AGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATC              <  SRR4302547.1347606/94‑1 (MQ=60)
                                                                                 AGCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATC              >  SRR4302547.30081/1‑94 (MQ=60)
                                                                                  GCAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCA             <  SRR4302547.4959426/94‑1 (MQ=60)
                                                                                  GCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCA             >  SRR4302547.2990094/1‑94 (MQ=60)
                                                                                   CAGCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCAT            >  SRR4302547.5684919/1‑94 (MQ=60)
                                                                                    AGCTGCAGCGGCTTTTGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATG           <  SRR4302547.561773/94‑1 (MQ=60)
                                                                                     GCTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGA          <  SRR4302547.1858002/94‑1 (MQ=60)
                                                                                      CTGCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGAT         <  SRR4302547.6309653/94‑1 (MQ=60)
                                                                                        GCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCT       <  SRR4302547.6439136/94‑1 (MQ=60)
                                                                                        GCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCT       <  SRR4302547.3584695/94‑1 (MQ=60)
                                                                                        GCAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCT       <  SRR4302547.4171899/94‑1 (MQ=60)
                                                                                         CAGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTT      <  SRR4302547.1659512/94‑1 (MQ=60)
                                                                                         CAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTT      <  SRR4302547.6350721/94‑1 (MQ=60)
                                                                                          AGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTG     >  SRR4302547.6484248/1‑94 (MQ=60)
                                                                                          AGCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTG     <  SRR4302547.1164197/94‑1 (MQ=60)
                                                                                           GCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTGT    <  SRR4302547.6260469/94‑1 (MQ=60)
                                                                                           GCGGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTGT    >  SRR4302547.829339/1‑94 (MQ=60)
                                                                                            CGGCTTTGGCGTGAGACAGAGATCCAGCCAGGATTGCGTTGGCGCCGAATTTGTATTGGTTTTCGGTGCCGTCCAGGTCGATCATTATCTAGTC   <  SRR4302547.4252646/94‑1 (MQ=60)
                                                                                             GGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTGTCA  >  SRR4302547.309914/1‑94 (MQ=60)
                                                                                             GGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTGTCA  >  SRR4302547.3654697/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTGTTTTCGGTGCCGTCCAGGTCGATCATGATCTTGTCA  >  NC_000913/2905508‑2905694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑