Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,490	C→T	A67T (GCT→ACT)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,490	0	C	T	84.9%	632.5 / 84.2	231	A67T (GCT→ACT)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand): ref base C (19/16); new base T (94/102); total (113/118)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

Only 100 of 243 total aligned reads displayed.

AGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTC  >  NC_000913/2907400‑2907581

 gTAGATACGACCCGTGGTGAAGGTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGcc                                                                                          <  1:5464451/93‑1 (MQ=255)

  tAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCg                                                                                         <  1:506389/93‑1 (MQ=255)

  tAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCg                                                                                         <  1:6636686/93‑1 (MQ=255)

   aGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGt                                                                                        <  2:6098378/93‑1 (MQ=255)

    aaTACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTc                                                                                       <  1:1435538/92‑1 (MQ=255)

      tACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCtt                                                                                     >  2:3730233/1‑93 (MQ=255)

       aCGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTc                                                                                    <  2:4388959/93‑1 (MQ=255)

       aCGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTc                                                                                    >  1:6432183/1‑93 (MQ=255)

       aCGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTc                                                                                    <  1:2094340/93‑1 (MQ=255)

       aCGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTc                                                                                    >  2:4867949/1‑93 (MQ=255)

        cGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCa                                                                                   <  1:254209/93‑1 (MQ=255)

         gACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAg                                                                                  >  2:1553594/1‑93 (MQ=255)

          gcccGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAGACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGt                                                                                 <  2:5772982/92‑1 (MQ=255)

          aCCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTTGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGt                                                                                 >  1:4742088/1‑93 (MQ=255)

           cccGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCTGTTTCAGTGCCGTCTTCAGTa                                                                                >  2:6219350/1‑93 (MQ=255)

           cccGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTa                                                                                >  1:4948112/1‑93 (MQ=255)

            ccGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTaa                                                                               <  2:4189534/93‑1 (MQ=255)

             cGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAAc                                                                              >  2:4915411/1‑93 (MQ=255)

              gtggtgAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACg                                                                             <  2:4986905/93‑1 (MQ=255)

               tggtgAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGa                                                                            >  2:513115/1‑93 (MQ=255)

                ggtgAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGaa                                                                           <  2:6570237/93‑1 (MQ=255)

                  tgAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAaca                                                                         >  2:1390134/1‑93 (MQ=255)

                   gAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAacac                                                                        >  1:6528568/1‑93 (MQ=255)

                   gAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAacac                                                                        >  1:4146575/1‑93 (MQ=255)

                    aaGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACt                                                                       >  1:37597/1‑93 (MQ=255)

                    aaGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGACATCTTCAGTAACGAACACt                                                                       >  2:2392675/1‑93 (MQ=255)

                      gttgttGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTc                                                                     <  1:2725660/93‑1 (MQ=255)

                      gttgttGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTc                                                                     <  1:5988004/93‑1 (MQ=255)

                       ttgttgCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTcc                                                                    <  1:729742/93‑1 (MQ=255)

                            gcggcgGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCgtg                                                               <  2:1579075/93‑1 (MQ=255)

                              ggcggcTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGtt                                                             <  1:84065/93‑1 (MQ=255)

                              ggcggaTCATTTTGCTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACCCTTCCCCGTGtt                                                             <  1:2434359/93‑1 (MQ=255)

                                cggcTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTgg                                                           >  1:5254997/1‑93 (MQ=255)

                                cggcTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTgg                                                           >  1:4119655/1‑93 (MQ=255)

                                 ggcTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGa                                                          >  2:2242228/1‑93 (MQ=255)

                                  gcTCATTTTGGTACGAATGAAACGCTCGTAGTGCCTCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGAt                                                         <  2:326237/93‑1 (MQ=255)

                                  gcTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGAt                                                         >  1:15352/1‑93 (MQ=255)

                                  gcTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGAt                                                         <  2:3980709/93‑1 (MQ=255)

                                   cTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGAtt                                                        >  1:3021892/1‑93 (MQ=255)

                                    tCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTg                                                       <  2:2979428/93‑1 (MQ=255)

                                    tCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTg                                                       <  2:4820445/93‑1 (MQ=255)

                                       ttttGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGc                                                    >  1:4478385/1‑93 (MQ=255)

                                       ttttGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGc                                                    <  2:1413361/93‑1 (MQ=255)

                                        tttGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCt                                                   <  2:5661764/93‑1 (MQ=255)

                                         ttGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTc                                                  >  1:6161811/1‑93 (MQ=255)

                                         ttGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTc                                                  <  2:5288851/93‑1 (MQ=255)

                                         ttGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTc                                                  <  2:2306172/93‑1 (MQ=255)

                                          tGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGGTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCa                                                 >  2:6340623/1‑93 (MQ=255)

                                           ggTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAt                                                <  1:177313/93‑1 (MQ=255)

                                           ggTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAt                                                <  2:1929539/93‑1 (MQ=255)

                                           ggTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAt                                                >  1:3228194/1‑93 (MQ=255)

                                            gTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATa                                               >  2:3405078/1‑93 (MQ=255)

                                             tACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAg                                              <  2:3517561/93‑1 (MQ=255)

                                               cGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTa                                            <  2:1225163/93‑1 (MQ=255)

                                               cGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTa                                            <  2:4560310/93‑1 (MQ=255)

                                                 agtGAACCACTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACCCTTCCCCGTGTTGGATTGGGCTCATAGTAcc                                          <  2:5903806/91‑1 (MQ=255)

                                                 aaTGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTAcc                                          >  1:1002184/1‑93 (MQ=255)

                                                    gAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTgg                                       >  1:2805903/1‑93 (MQ=255)

                                                     aaaCGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGa                                      <  2:3616118/93‑1 (MQ=255)

                                                     aaaCGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGa                                      >  2:5576739/1‑93 (MQ=255)

                                                     aaaCGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGa                                      <  1:4713560/93‑1 (MQ=255)

                                                         gCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGa                                  <  2:4798062/93‑1 (MQ=255)

                                                         gCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGa                                  >  1:1661028/1‑93 (MQ=255)

                                                            cGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGt                               <  1:5556415/93‑1 (MQ=255)

                                                              tAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTg                             >  1:1821499/1‑93 (MQ=255)

                                                               aGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGa                            <  2:5364417/93‑1 (MQ=255)

                                                               aGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGa                            >  2:1983282/1‑93 (MQ=255)

                                                                gTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGAt                           >  1:4318517/1‑93 (MQ=255)

                                                                gTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGAt                           <  2:5106493/93‑1 (MQ=255)

                                                                gTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGAt                           <  2:4342431/93‑1 (MQ=255)

                                                                 tGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATg                          <  1:2591185/93‑1 (MQ=255)

                                                                 tGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATg                          <  2:4429122/93‑1 (MQ=255)

                                                                   ccccAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTa                        <  1:3598611/93‑1 (MQ=255)

                                                                     ccaggtccaggtCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATTTACg                      >  1:2284084/1‑93 (MQ=255)

                                                                     ccaggtccaggtCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACg                      <  2:46017/93‑1 (MQ=255)

                                                                     ccaggtccaggtCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACg                      >  1:2231029/1‑93 (MQ=255)

                                                                     ccaggtccaggtCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTACCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGGTGATGTACg                      >  2:1194078/1‑93 (MQ=255)

                                                                      caggtccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACgg                     <  2:917175/93‑1 (MQ=255)

                                                                      caggtccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACgg                     <  1:2641332/93‑1 (MQ=255)

                                                                      caggtccaggtcGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACgg                     >  1:5179114/1‑93 (MQ=255)

                                                                       aggtccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGa                    <  2:6455695/93‑1 (MQ=255)

                                                                        ggtccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGAt                   <  2:3626373/93‑1 (MQ=255)

                                                                         gtccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATc                  <  2:4166383/93‑1 (MQ=255)

                                                                          tccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATcc                 <  1:1940284/93‑1 (MQ=255)

                                                                          tccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATcc                 <  2:5203200/93‑1 (MQ=255)

                                                                          tccaggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATcc                 <  2:5527812/93‑1 (MQ=255)

                                                                           ccaggtcGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCa                <  1:5513390/93‑1 (MQ=255)

                                                                              ggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGtt             >  2:4280710/1‑93 (MQ=255)

                                                                              ggtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGtt             <  2:831747/93‑1 (MQ=255)

                                                                               gtcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGttt            >  2:702334/1‑93 (MQ=255)

                                                                                tcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTc           >  2:2479197/1‑93 (MQ=255)

                                                                                tcGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTc           <  1:1181922/93‑1 (MQ=255)

                                                                                 cGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCa          <  1:986675/93‑1 (MQ=255)

                                                                                  ggTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCat         >  1:5836596/1‑93 (MQ=255)

                                                                                  cgTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCat         <  1:3906509/92‑1 (MQ=255)

                                                                                   gTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCatg        <  1:1346446/93‑1 (MQ=255)

                                                                                      tCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCatgatg     >  1:1030246/1‑93 (MQ=255)

                                                                                       cAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATgg    >  1:5605293/1‑93 (MQ=255)

                                                                                       cAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATgg    <  2:446676/93‑1 (MQ=255)

                                                                                         gcgcCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTc  >  2:2121840/1‑93 (MQ=255)

AGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTC  >  NC_000913/2907400‑2907581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCAC  >  NC_000913/2907397‑2907583

CAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGT                                                                                               <  SRR4302547.6426621/94‑1 (MQ=60)

CAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGT                                                                                               <  SRR4302547.1673322/94‑1 (MQ=60)

CAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGCGCGGTTTCAGT                                                                                               >  SRR4302547.5473388/1‑94 (MQ=60)

 AGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTG                                                                                              >  SRR4302547.2767610/1‑94 (MQ=60)

 AGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTG                                                                                              >  SRR4302547.3216351/1‑94 (MQ=60)

   AGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCC                                                                                            <  SRR4302547.2867215/94‑1 (MQ=60)

   AGTAGATACGACCCGTGGTGAAGTTGGTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCC                                                                                            <  SRR4302547.3439448/94‑1 (MQ=60)

    GTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCG                                                                                           <  SRR4302547.2284991/94‑1 (MQ=60)

    GTAGATACGACCCGTGGTGAAGGTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCG                                                                                           <  SRR4302547.5577336/94‑1 (MQ=60)

     TAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGT                                                                                          <  SRR4302547.515587/94‑1 (MQ=60)

     TAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGT                                                                                          >  SRR4302547.1768477/1‑94 (MQ=60)

     TAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGT                                                                                          <  SRR4302547.6774832/94‑1 (MQ=60)

      AGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGGCGTC                                                                                         >  SRR4302547.1885976/1‑94 (MQ=60)

       AATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCT                                                                                        <  SRR4302547.1464237/94‑1 (MQ=60)

         TACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTC                                                                                      >  SRR4302547.6566253/1‑94 (MQ=60)

          ACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCA                                                                                     <  SRR4302547.2136089/94‑1 (MQ=60)

           CGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAG                                                                                    <  SRR4302547.258963/94‑1 (MQ=60)

            GACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTTGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGT                                                                                   >  SRR4302547.4840361/1‑94 (MQ=60)

            GACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGT                                                                                   >  SRR4302547.1513899/1‑94 (MQ=60)

            GACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGT                                                                                   >  SRR4302547.1786460/1‑94 (MQ=60)

             ACCCGTGGTGAAGTTGTTGCGGCGGCTTATTTTGGTACCAATGAAACGCTTGTTGTGCCCCCGGTCCCGGTCGGTTTCAGTTCCGTCCTCAGTA                                                                                  <  SRR4302547.5626640/94‑1 (MQ=55)

             ACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTA                                                                                  >  SRR4302547.5050581/1‑94 (MQ=60)

             ACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTA                                                                                  >  SRR4302547.5298387/1‑94 (MQ=60)

              CCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAA                                                                                 >  SRR4302547.2768074/1‑94 (MQ=60)

               CCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAAC                                                                                >  SRR4302547.6136284/1‑94 (MQ=60)

               CCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAAC                                                                                <  SRR4302547.2801907/94‑1 (MQ=60)

                CGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACG                                                                               >  SRR4302547.3964682/1‑94 (MQ=60)

                 GTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAATGCCCCAGGTCCAGGTCGGTTTCAGTTCCGTCTTCAGTAACGA                                                                              >  SRR4302547.6740366/1‑94 (MQ=60)

                    GTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACA                                                                           >  SRR4302547.4751476/1‑94 (MQ=60)

                     TGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACAC                                                                          >  SRR4302547.6664459/1‑94 (MQ=60)

                     TGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACAC                                                                          >  SRR4302547.4230991/1‑94 (MQ=60)

                      GAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACT                                                                         <  SRR4302547.3134799/94‑1 (MQ=60)

                      GAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACT                                                                         >  SRR4302547.3018202/1‑94 (MQ=60)

                      GAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACT                                                                         >  SRR4302547.38295/1‑94 (MQ=60)

                      GAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACT                                                                         <  SRR4302547.6614470/94‑1 (MQ=60)

                        AGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTC                                                                       >  SRR4302547.5241255/1‑94 (MQ=60)

                         GTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCC                                                                      <  SRR4302547.6112241/94‑1 (MQ=60)

                         GTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCC                                                                      <  SRR4302547.2780387/94‑1 (MQ=60)

                          TTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCC                                                                     <  SRR4302547.743357/94‑1 (MQ=60)

                           TGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCC                                                                    >  SRR4302547.6046368/1‑94 (MQ=60)

                            GTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCG                                                                   >  SRR4302547.4401797/1‑94 (MQ=60)

                              TGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACCCCTCCCCGTG                                                                 >  SRR4302547.4014404/1‑94 (MQ=60)

                               GCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGT                                                                >  SRR4302547.4270808/1‑94 (MQ=60)

                                 GGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTG                                                              <  SRR4302547.85615/94‑1 (MQ=60)

                                 GGCGGATCATTTTGCTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACCCTTCCCCGTGTTG                                                              <  SRR4302547.2483606/94‑1 (MQ=60)

                                  GCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGG                                                             >  SRR4302547.5363193/1‑94 (MQ=60)

                                  GCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGG                                                             <  SRR4302547.964283/94‑1 (MQ=60)

                                  GCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGG                                                             >  SRR4302547.4203380/1‑94 (MQ=60)

                                   CGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGA                                                            <  SRR4302547.3541468/94‑1 (MQ=60)

                                    GGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGAT                                                           >  SRR4302547.15636/1‑94 (MQ=60)

                                     GCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATT                                                          <  SRR4302547.4928599/94‑1 (MQ=60)

                                     GCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATT                                                          <  SRR4302547.3799628/94‑1 (MQ=60)

                                     GCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATT                                                          >  SRR4302547.3081981/1‑94 (MQ=60)

                                      CTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTG                                                         >  SRR4302547.6159091/1‑94 (MQ=60)

                                         ATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGC                                                      >  SRR4302547.4570905/1‑94 (MQ=60)

                                         ATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGC                                                      >  SRR4302547.1032397/1‑94 (MQ=60)

                                           TTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTC                                                    >  SRR4302547.6289691/1‑94 (MQ=60)

                                             TGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAT                                                  >  SRR4302547.5900007/1‑94 (MQ=60)

                                             TGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAT                                                  >  SRR4302547.3292625/1‑94 (MQ=60)

                                             TGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAT                                                  >  SRR4302547.789656/1‑94 (MQ=60)

                                             TGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAT                                                  >  SRR4302547.109564/1‑94 (MQ=60)

                                             TGGTACGAATGAAACGCTCGTAGTGCCCAAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCAT                                                  >  SRR4302547.1206801/1‑94 (MQ=60)

                                              GGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATA                                                 <  SRR4302547.180607/94‑1 (MQ=60)

                                                TACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGT                                               >  SRR4302547.2539458/1‑94 (MQ=60)

                                                 ACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTA                                              <  SRR4302547.5680123/94‑1 (MQ=60)

                                                 ACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTA                                              >  SRR4302547.1387658/1‑94 (MQ=60)

                                                  CGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTAC                                             >  SRR4302547.2731204/1‑94 (MQ=60)

                                                   GAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACC                                            >  SRR4302547.1021736/1‑94 (MQ=60)

                                                    AATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGGCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCT                                           >  SRR4302547.563670/1‑94 (MQ=60)

                                                      TGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGG                                         >  SRR4302547.2070331/1‑94 (MQ=60)

                                                      TGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGG                                         >  SRR4302547.4228011/1‑94 (MQ=60)

                                                      TGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGG                                         >  SRR4302547.2862069/1‑94 (MQ=60)

                                                        AAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGAT                                       <  SRR4302547.4811144/94‑1 (MQ=60)

                                                         AACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATC                                      <  SRR4302547.2597546/94‑1 (MQ=60)

                                                           CGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGA                                    >  SRR4302547.1693779/1‑94 (MQ=60)

                                                             CTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACG                                  >  SRR4302547.1576803/1‑94 (MQ=60)

                                                               CGTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTT                                <  SRR4302547.5671031/94‑1 (MQ=60)

                                                                TTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTG                               <  SRR4302547.5747585/94‑1 (MQ=60)

                                                                GTAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTG                               <  SRR4302547.1557224/94‑1 (MQ=60)

                                                                GTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTG                               >  SRR4302547.1857511/1‑94 (MQ=60)

                                                                 TAGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGA                              <  SRR4302547.6408561/94‑1 (MQ=60)

                                                                  AGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGAT                             >  SRR4302547.5619578/1‑94 (MQ=60)

                                                                  AGTGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGAT                             >  SRR4302547.4407404/1‑94 (MQ=60)

                                                                    TGCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGT                           <  SRR4302547.2643451/94‑1 (MQ=60)

                                                                     GCCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTA                          <  SRR4302547.2342259/94‑1 (MQ=60)

                                                                      CCCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC                         <  SRR4302547.3671992/94‑1 (MQ=60)

                                                                       CCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATTTACG                        >  SRR4302547.2330326/1‑94 (MQ=60)

                                                                       CCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACG                        >  SRR4302547.1839467/1‑94 (MQ=60)

                                                                       CCCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACG                        <  SRR4302547.2005195/94‑1 (MQ=60)

                                                                       CCCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACG                        >  SRR4302547.2275997/1‑94 (MQ=60)

                                                                        CCAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGG                       >  SRR4302547.5065957/1‑94 (MQ=60)

                                                                        CCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGG                       >  SRR4302547.5285716/1‑94 (MQ=60)

                                                                        CCAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGG                       <  SRR4302547.5089537/94‑1 (MQ=60)

                                                                         CAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGA                      <  SRR4302547.2489363/94‑1 (MQ=60)

                                                                         CAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGA                      <  SRR4302547.2694494/94‑1 (MQ=60)

                                                                         CAGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGA                      >  SRR4302547.704059/1‑94 (MQ=60)

                                                                         CAGGTCCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGA                      >  SRR4302547.529034/1‑94 (MQ=60)

                                                                          AGGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGAT                     >  SRR4302547.4839580/1‑94 (MQ=60)

                                                                           GGTCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATC                    <  SRR4302547.5592880/94‑1 (MQ=60)

                                                                             TCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCA                  <  SRR4302547.1978765/94‑1 (MQ=60)

                                                                             TCCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATACA                  >  SRR4302547.4519792/1‑94 (MQ=60)

                                                                              CCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAG                 <  SRR4302547.5395294/94‑1 (MQ=60)

                                                                              CCAGGTCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAG                 >  SRR4302547.4172038/1‑94 (MQ=60)

                                                                              CCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAG                 <  SRR4302547.5627209/94‑1 (MQ=60)

                                                                              CCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAG                 >  SRR4302547.819735/1‑94 (MQ=60)

                                                                              CCAGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAG                 >  SRR4302547.2184549/1‑94 (MQ=60)

                                                                                AGGTCGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGAGGTACGGATCCAGTT               <  SRR4302547.6616790/94‑1 (MQ=60)

                                                                                   TCGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCA            <  SRR4302547.1205799/94‑1 (MQ=60)

                                                                                    CGGTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCAT           >  SRR4302547.2320093/1‑94 (MQ=60)

                                                                                    CGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCAT           >  SRR4302547.5957361/1‑94 (MQ=60)

                                                                                    CGGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCAT           <  SRR4302547.1005878/94‑1 (MQ=60)

                                                                                     CGTTTCAGCGCCGCCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATG          <  SRR4302547.3985952/94‑1 (MQ=60)

                                                                                      GTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGA         <  SRR4302547.1373475/94‑1 (MQ=60)

                                                                                      GTTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGCTGGATTTGGCTCATAGTACCTGGATCGACGTTGATGAACGGATCCCGGTTCATGA         >  SRR4302547.2990754/1‑94 (MQ=60)

                                                                                       TTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGAT        <  SRR4302547.1239352/94‑1 (MQ=60)

                                                                                       TTTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGAT        <  SRR4302547.2684563/94‑1 (MQ=60)

                                                                                        TTCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATG       >  SRR4302547.1050487/1‑94 (MQ=60)

                                                                                         TCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGG      <  SRR4302547.4797318/94‑1 (MQ=60)

                                                                                         TCAGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGG      >  SRR4302547.5720937/1‑94 (MQ=60)

                                                                                           AGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTC    <  SRR4302547.4919413/94‑1 (MQ=60)

                                                                                           AGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTC    >  SRR4302547.5089970/1‑94 (MQ=60)

                                                                                           AGTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTC    >  SRR4302547.115523/1‑94 (MQ=60)

                                                                                            GTGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGTATCCAGTTTCCTGATGGTCA   <  SRR4302547.6623646/94‑1 (MQ=60)

                                                                                             TGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCAC  <  SRR4302547.3411151/94‑1 (MQ=60)

CAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCAC  >  NC_000913/2907397‑2907583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`