Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,929,613 A→G V179V (GTA→GTG)  b2798 → Exo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,929,6130AG85.5% 583.8 / 76.0 228V179V (GTA→GTG) b2798Exo
Reads supporting (aligned to +/- strand):  ref base A (13/20);  new base G (89/106);  total (102/126)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

GGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGT  >  NC_000913/2929523‑2929705
                                                                                          |                                                                                            
ggATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGcc                                                                                            >  2:6542680/1‑93 (MQ=255)
   tGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCggg                                                                                         >  1:1073864/1‑93 (MQ=255)
   tGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCggg                                                                                         <  2:6245648/93‑1 (MQ=255)
     cgcCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGgtg                                                                                       <  1:6033721/93‑1 (MQ=255)
     cgcCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGgtg                                                                                       <  2:1490747/93‑1 (MQ=255)
      gctaTTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGgtgt                                                                                      <  2:669784/90‑1 (MQ=255)
      gcCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGgtgt                                                                                      >  1:5930361/1‑93 (MQ=255)
      gcCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGgtgt                                                                                      <  1:4270913/93‑1 (MQ=255)
       ccATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGtt                                                                                     <  1:440742/93‑1 (MQ=255)
       ccATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGtt                                                                                     >  1:5753203/1‑93 (MQ=255)
       ccATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGtt                                                                                     <  1:196042/93‑1 (MQ=255)
        cATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAATGTGCCGGGTGTTg                                                                                    >  1:1496143/1‑93 (MQ=255)
            tATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCggg                                                                                <  2:1068124/93‑1 (MQ=255)
            tATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCggg                                                                                <  1:3212829/93‑1 (MQ=255)
             aTCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGa                                                                               <  2:1453885/93‑1 (MQ=255)
                gATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATc                                                                            <  2:5251359/93‑1 (MQ=255)
                caTAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATc                                                                            <  1:2504922/92‑1 (MQ=255)
                 aTAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCg                                                                           >  2:4395417/1‑93 (MQ=255)
                   aaaGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGa                                                                         <  2:5186342/93‑1 (MQ=255)
                   aaaGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGa                                                                         <  1:110546/93‑1 (MQ=255)
                   aaaGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGa                                                                         <  2:4648658/93‑1 (MQ=255)
                     aGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGAcc                                                                       <  1:3844575/93‑1 (MQ=255)
                      gAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCa                                                                      >  1:2593202/1‑93 (MQ=255)
                      gAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCa                                                                      >  1:4335035/1‑93 (MQ=255)
                      gAATGTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAAGCGTACCa                                                                      >  1:3203877/1‑93 (MQ=255)
                        aTTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCaaa                                                                    >  1:372454/1‑93 (MQ=255)
                         tttGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCaaaa                                                                   <  2:2375586/93‑1 (MQ=255)
                         tttGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCaaaa                                                                   >  2:4314429/1‑93 (MQ=255)
                         tttGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCaaaa                                                                   <  1:5455811/93‑1 (MQ=255)
                           tGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAg                                                                 <  2:4030587/93‑1 (MQ=255)
                           tGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAg                                                                 >  1:2125649/1‑93 (MQ=255)
                            ggCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAgc                                                                <  2:5787250/93‑1 (MQ=255)
                              cGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGCATCGGACCAAAAAgcgc                                                              >  1:1031621/1‑93 (MQ=255)
                              cGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAgcgc                                                              >  1:85481/1‑93 (MQ=255)
                               gTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGcc                                                             <  1:6232355/93‑1 (MQ=255)
                                ttCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGccc                                                            >  1:3535391/1‑92 (MQ=255)
                                   aaacGCAGCAGTTGCTCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGc                                                         <  1:3111715/90‑1 (MQ=255)
                                   aaCCGCAGCAGTTGCCCGATTACTGTGGACTTGCGGGGATCAGAAGTTCAAAGGTGCCGGGTGTTACGGTAATCGGACCAAAAAGCGCCACGc                                                         <  1:5156273/93‑1 (MQ=255)
                                    aCCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCa                                                        >  2:5692039/1‑93 (MQ=255)
                                    aCCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCa                                                        <  1:1600099/93‑1 (MQ=255)
                                       gcagcaGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgct                                                     >  2:1942143/1‑93 (MQ=255)
                                        cagcagTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgctg                                                    <  1:4703144/93‑1 (MQ=255)
                                        cagcagTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgctg                                                    >  2:553655/1‑93 (MQ=255)
                                         agcagTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgctgc                                                   <  1:618202/93‑1 (MQ=255)
                                          gcagTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgctgct                                                  >  2:3224608/1‑93 (MQ=255)
                                          gcagTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgctgct                                                  >  2:638700/1‑93 (MQ=255)
                                          gcagTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgctgct                                                  <  1:919735/93‑1 (MQ=255)
                                          gcagTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGCGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAgctgct                                                  <  2:5830275/93‑1 (MQ=255)
                                            agTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTgg                                                >  1:3481206/1‑93 (MQ=255)
                                             gTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGt                                               <  1:3041616/93‑1 (MQ=255)
                                             gTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGt                                               <  1:4567005/93‑1 (MQ=255)
                                              ttGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTc                                              >  1:2004233/1‑93 (MQ=255)
                                                 cccGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAg                                           <  1:27157/93‑1 (MQ=255)
                                                  ccGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGt                                          >  2:4607211/1‑93 (MQ=255)
                                                   cGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGtt                                         <  2:2151568/93‑1 (MQ=255)
                                                    gagtACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGttt                                        <  2:1800618/90‑1 (MQ=255)
                                                    gATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGttt                                        <  1:6109101/93‑1 (MQ=255)
                                                     aTTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTc                                       >  1:4938281/1‑93 (MQ=255)
                                                     aTTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTc                                       >  1:1896646/1‑93 (MQ=255)
                                                     aTTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTc                                       >  2:2871216/1‑93 (MQ=255)
                                                      ttACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCa                                      <  2:5226554/93‑1 (MQ=255)
                                                       tACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCag                                     >  1:3074640/1‑93 (MQ=255)
                                                        tcTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCaga                                    <  1:6643392/92‑1 (MQ=255)
                                                        tcTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCaga                                    <  1:4125578/92‑1 (MQ=255)
                                                        aCTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCaga                                    <  2:6597102/93‑1 (MQ=255)
                                                         cTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCagag                                   <  2:3975494/93‑1 (MQ=255)
                                                            gggACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCt                                <  1:6276671/93‑1 (MQ=255)
                                                             ggACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTg                               <  1:2244802/93‑1 (MQ=255)
                                                              gACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTgg                              >  2:2578705/1‑93 (MQ=255)
                                                               aCTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGa                             <  1:4041716/93‑1 (MQ=255)
                                                                cTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGaa                            >  2:6149887/1‑93 (MQ=255)
                                                                  tGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAgg                          >  2:1782116/1‑93 (MQ=255)
                                                                  tGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAgg                          <  2:1388591/93‑1 (MQ=255)
                                                                  tGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAgg                          >  2:810504/1‑93 (MQ=255)
                                                                  tGCGGGGATAAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACTAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAgg                          <  1:3989410/93‑1 (MQ=255)
                                                                    cGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGa                        <  1:5083590/93‑1 (MQ=255)
                                                                    cGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGAAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGa                        <  2:1546531/93‑1 (MQ=255)
                                                                     ggggATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGat                       >  2:457247/1‑93 (MQ=255)
                                                                      gggATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGata                      >  2:3175080/1‑93 (MQ=255)
                                                                      gggATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGata                      >  2:748535/1‑93 (MQ=255)
                                                                      gggATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGata                      <  2:878743/93‑1 (MQ=255)
                                                                       ggATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGatat                     >  1:5220725/1‑93 (MQ=255)
                                                                        gATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGatata                    >  2:5486426/1‑93 (MQ=255)
                                                                         aTCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGatatat                   >  2:1163722/1‑93 (MQ=255)
                                                                         aTCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGatatat                   >  1:5329651/1‑93 (MQ=255)
                                                                           cagcagTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGatatgtaa                 <  2:2943432/93‑5 (MQ=255)
                                                                           cagcagTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATga                 <  1:1545830/93‑1 (MQ=255)
                                                                           cagcagTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATga                 <  1:322838/93‑1 (MQ=255)
                                                                             gcagTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATgaga               >  2:2197105/1‑93 (MQ=255)
                                                                             gcagTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATgaga               >  1:1281139/1‑93 (MQ=255)
                                                                             gcagTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATgaga               >  2:4916527/1‑93 (MQ=255)
                                                                                gTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATc            <  2:2535701/93‑1 (MQ=255)
                                                                                 ttCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCt           <  2:199775/93‑1 (MQ=255)
                                                                                 ttCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCt           <  2:2824135/93‑1 (MQ=255)
                                                                                   cAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTgg         >  2:4989019/1‑93 (MQ=255)
                                                                                    aaaGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGa        <  2:3947163/93‑1 (MQ=255)
                                                                                    aaaGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGa        <  1:2150744/93‑1 (MQ=255)
                                                                                     aaGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGAt       <  2:3536469/93‑1 (MQ=255)
                                                                                      aGGTGCCGGGTGTTGCGAGAATCGGACAAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATg      <  1:4069490/93‑1 (MQ=255)
                                                                                          aCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGt  <  1:4008041/93‑1 (MQ=255)
                                                                                          |                                                                                            
GGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGT  >  NC_000913/2929523‑2929705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGTT  >  NC_000913/2929521‑2929706
                                                                                            |                                                                                             
CTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGC                                                                                              >  SRR4302547.1276210/1‑94 (MQ=60)
CTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGC                                                                                              >  SRR4302547.4922981/1‑94 (MQ=60)
 TGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCC                                                                                             <  SRR4302547.2709530/94‑1 (MQ=60)
 TGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCC                                                                                             <  SRR4302547.6219573/94‑1 (MQ=60)
    ATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGG                                                                                          >  SRR4302547.1095294/1‑94 (MQ=60)
    ATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGG                                                                                          >  SRR4302547.429794/1‑94 (MQ=60)
     TGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGT                                                                                         >  SRR4302547.4194393/1‑94 (MQ=60)
      GCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGCG                                                                                        >  SRR4302547.1104005/1‑94 (MQ=60)
       CGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGT                                                                                       <  SRR4302547.6158935/94‑1 (MQ=60)
       CGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGT                                                                                       >  SRR4302547.6053310/1‑94 (MQ=60)
        GCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTT                                                                                      <  SRR4302547.2189325/94‑1 (MQ=60)
        GCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTT                                                                                      >  SRR4302547.5872139/1‑94 (MQ=60)
        GCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTT                                                                                      <  SRR4302547.1854633/94‑1 (MQ=60)
        GCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTT                                                                                      <  SRR4302547.4358568/94‑1 (MQ=60)
         CCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAATGTGCCGGGTGTTG                                                                                     >  SRR4302547.1525990/1‑94 (MQ=60)
         CCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTG                                                                                     <  SRR4302547.448960/94‑1 (MQ=60)
         CCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTG                                                                                     <  SRR4302547.199706/94‑1 (MQ=60)
           ATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCG                                                                                   >  SRR4302547.4520584/1‑94 (MQ=60)
            TTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGG                                                                                  <  SRR4302547.4211717/94‑1 (MQ=60)
             TTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGG                                                                                 <  SRR4302547.2413460/94‑1 (MQ=60)
              TATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGA                                                                                <  SRR4302547.3276921/94‑1 (MQ=60)
               ATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAA                                                                               <  SRR4302547.4527719/94‑1 (MQ=60)
                TCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAAT                                                                              <  SRR4302547.4095526/94‑1 (MQ=60)
                  CATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCG                                                                            <  SRR4302547.2555544/94‑1 (MQ=60)
                    TAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGA                                                                          >  SRR4302547.3168552/1‑94 (MQ=60)
                    TAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGA                                                                          <  SRR4302547.4489895/94‑1 (MQ=60)
                     AAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGAC                                                                         <  SRR4302547.112565/94‑1 (MQ=60)
                       AGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCA                                                                       >  SRR4302547.2645497/1‑94 (MQ=60)
                       AGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCA                                                                       >  SRR4302547.4424210/1‑94 (MQ=60)
                       AGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCA                                                                       <  SRR4302547.3922857/94‑1 (MQ=60)
                       AGAATGTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAAGCGTACCA                                                                       >  SRR4302547.3267775/1‑94 (MQ=60)
                         AATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAA                                                                     >  SRR4302547.379355/1‑94 (MQ=60)
                         AATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAA                                                                     >  SRR4302547.1065402/1‑94 (MQ=60)
                          ATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAA                                                                    <  SRR4302547.3580150/94‑1 (MQ=60)
                          ATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAA                                                                    <  SRR4302547.1870960/94‑1 (MQ=60)
                           TTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAA                                                                   <  SRR4302547.5568535/94‑1 (MQ=60)
                           TTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAA                                                                   >  SRR4302547.6245510/1‑94 (MQ=60)
                            TTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAG                                                                  >  SRR4302547.2168008/1‑94 (MQ=60)
                            TTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAG                                                                  >  SRR4302547.6371294/1‑94 (MQ=60)
                              GGCGTTCAACCGCAGCAGTTGGCCGAATCCTGGGGACTTGCGGGGATCAGCAGTTCAAAGGGGCCGGGCGTTGCGGGAATCGGAtcaaacagcc                                                                >  SRR4302547.1474533/1‑84 (MQ=60)
                              GGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCG                                                                <  SRR4302547.2163310/94‑1 (MQ=60)
                               GCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGCATCGGACCAAAAAGCGC                                                               >  SRR4302547.1051903/1‑94 (MQ=60)
                               GCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGC                                                               >  SRR4302547.87061/1‑94 (MQ=60)
                                TGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCC                                                              >  SRR4302547.2500743/1‑94 (MQ=60)
                                 GTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCC                                                             >  SRR4302547.3607572/1‑94 (MQ=60)
                                 GTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCA                                                             <  SRR4302547.6361827/94‑1 (MQ=60)
                                 GTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCA                                                             <  SRR4302547.630418/94‑1 (MQ=60)
                                  TTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCAC                                                            <  SRR4302547.5094403/94‑1 (MQ=60)
                                   TCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACG                                                           <  SRR4302547.2005895/94‑1 (MQ=60)
                                    CAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCGCGC                                                          >  SRR4302547.3261385/1‑94 (MQ=60)
                                    CAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGC                                                          >  SRR4302547.6598851/1‑94 (MQ=60)
                                     AACCGCAGCAGTTGCCCGATTACTGTGGACTTGCGGGGATCAGAAGTTCAAAGGTGCCGGGTGTTACGGTAATCGGACCAAAAAGCGCCACGCA                                                         <  SRR4302547.5262451/94‑1 (MQ=60)
                                     AACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCA                                                         <  SRR4302547.5262450/94‑1 (MQ=60)
                                     AACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCA                                                         >  SRR4302547.5419390/1‑94 (MQ=60)
                                     AACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCA                                                         >  SRR4302547.2754451/1‑94 (MQ=60)
                                     AACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCA                                                         <  SRR4302547.4078543/94‑1 (MQ=60)
                                     AAACGCAGCAGTTGCTCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCA                                                         <  SRR4302547.3173379/94‑1 (MQ=60)
                                      ACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAG                                                        <  SRR4302547.1631842/94‑1 (MQ=60)
                                        CGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCT                                                      <  SRR4302547.6110685/94‑1 (MQ=60)
                                        CGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCT                                                      >  SRR4302547.3609049/1‑94 (MQ=60)
                                          CAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGC                                                    <  SRR4302547.4800547/94‑1 (MQ=60)
                                           AGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCT                                                   <  SRR4302547.629651/94‑1 (MQ=60)
                                           AGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCT                                                   >  SRR4302547.928892/1‑94 (MQ=60)
                                            GCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTG                                                  <  SRR4302547.5895819/94‑1 (MQ=60)
                                            GCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTG                                                  <  SRR4302547.937491/94‑1 (MQ=60)
                                             CAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGG                                                 >  SRR4302547.3552221/1‑94 (MQ=60)
                                              AGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGT                                                >  SRR4302547.97618/1‑94 (MQ=60)
                                              AGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGAGCCACGCAGCTGCTGGT                                                >  SRR4302547.2257033/1‑94 (MQ=60)
                                               GTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTC                                               <  SRR4302547.6263003/94‑1 (MQ=60)
                                               GTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTC                                               <  SRR4302547.4661501/94‑1 (MQ=60)
                                               GTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTC                                               <  SRR4302547.3102150/94‑1 (MQ=60)
                                               GTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTC                                               >  SRR4302547.2044122/1‑94 (MQ=60)
                                                TTGCCCGATTACTGGGGAGTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCG                                              <  SRR4302547.3039717/94‑1 (MQ=60)
                                                TTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCG                                              <  SRR4302547.5298118/94‑1 (MQ=60)
                                                  GCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAG                                            >  SRR4302547.4407933/1‑94 (MQ=60)
                                                   CCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGT                                           <  SRR4302547.27654/94‑1 (MQ=60)
                                                    CCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTT                                          >  SRR4302547.4105987/1‑94 (MQ=60)
                                                     CGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTT                                         <  SRR4302547.6506768/94‑1 (MQ=60)
                                                      GATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTC                                        <  SRR4302547.1954892/94‑1 (MQ=60)
                                                      GATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTC                                        >  SRR4302547.1934154/1‑94 (MQ=60)
                                                      GATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTC                                        >  SRR4302547.3130903/1‑94 (MQ=60)
                                                      GATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTC                                        <  SRR4302547.6235816/94‑1 (MQ=60)
                                                      GATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTC                                        >  SRR4302547.5040564/1‑94 (MQ=60)
                                                        TTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAG                                      >  SRR4302547.3135722/1‑94 (MQ=60)
                                                         TACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAATAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGA                                     <  SRR4302547.5415801/94‑1 (MQ=60)
                                                          TCTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAG                                    <  SRR4302547.4209481/94‑1 (MQ=60)
                                                          TCTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAG                                    <  SRR4302547.6781702/94‑1 (MQ=60)
                                                            TGGGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGGGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTcctgctcgcgttccagatgt                                  >  SRR4302547.6014472/1‑74 (MQ=60)
                                                              GGGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTG                                <  SRR4302547.6407069/94‑1 (MQ=60)
                                                               GGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGG                               <  SRR4302547.2290086/94‑1 (MQ=60)
                                                               GGACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGG                               <  SRR4302547.3875789/94‑1 (MQ=60)
                                                                GACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGA                              <  SRR4302547.327793/94‑1 (MQ=60)
                                                                 ACTTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAA                             <  SRR4302547.4123801/94‑1 (MQ=60)
                                                                   TTGCGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGG                           <  SRR4302547.1317866/94‑1 (MQ=60)
                                                                   TTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGG                           <  SRR4302547.3439377/94‑1 (MQ=60)
                                                                    TGCGGGGATAAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACTAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGG                          <  SRR4302547.4070534/94‑1 (MQ=60)
                                                                      CGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGAT                        <  SRR4302547.5188463/94‑1 (MQ=60)
                                                                      CGGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGAT                        >  SRR4302547.1191444/1‑94 (MQ=60)
                                                                       GGGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATA                       <  SRR4302547.1361073/94‑1 (MQ=60)
                                                                        GGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATAT                      <  SRR4302547.55465/94‑1 (MQ=60)
                                                                        GGGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATAT                      >  SRR4302547.5328165/1‑94 (MQ=60)
                                                                         GGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATA                     >  SRR4302547.3268274/1‑94 (MQ=60)
                                                                         GGATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATA                     <  SRR4302547.3229437/94‑1 (MQ=60)
                                                                          GATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATAT                    >  SRR4302547.1621334/1‑94 (MQ=60)
                                                                          GATCAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATAT                    <  SRR4302547.1527741/94‑1 (MQ=60)
                                                                          GATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATAT                    <  SRR4302547.2147288/94‑1 (MQ=60)
                                                                          GATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATAT                    >  SRR4302547.5439509/1‑94 (MQ=60)
                                                                             CAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAG                 <  SRR4302547.5020838/94‑1 (MQ=60)
                                                                             CAGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAG                 <  SRR4302547.1576606/94‑1 (MQ=60)
                                                                             CAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAG                 <  SRR4302547.328842/94‑1 (MQ=60)
                                                                              AGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGA                >  SRR4302547.5798482/1‑94 (MQ=60)
                                                                              AGCAGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGA                >  SRR4302547.3438157/1‑94 (MQ=60)
                                                                              AGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGA                >  SRR4302547.1306935/1‑94 (MQ=60)
                                                                                 AGTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATC             >  SRR4302547.980318/1‑94 (MQ=60)
                                                                                  GTTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCT            >  SRR4302547.3961176/1‑94 (MQ=60)
                                                                                   TTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTG           <  SRR4302547.4132107/94‑1 (MQ=60)
                                                                                   TTCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTG           <  SRR4302547.769339/94‑1 (MQ=60)
                                                                                    TCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGG          >  SRR4302547.5145857/1‑94 (MQ=60)
                                                                                    TCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGG          <  SRR4302547.3862882/94‑1 (MQ=60)
                                                                                    TCAAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGG          <  SRR4302547.2600916/94‑1 (MQ=60)
                                                                                      AAAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGAT        >  SRR4302547.4695557/1‑94 (MQ=60)
                                                                                      AAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGAT        <  SRR4302547.2193671/94‑1 (MQ=60)
                                                                                       AAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATG       <  SRR4302547.6234171/94‑1 (MQ=60)
                                                                                       AAGGTGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATG       >  SRR4302547.2828408/1‑94 (MQ=60)
                                                                                        AGGTGCCGGGTGTTGCGAGAATCGGACAAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGC      <  SRR4302547.4152154/94‑1 (MQ=60)
                                                                                           TGCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGT   >  SRR4302547.2160689/1‑94 (MQ=60)
                                                                                            GCCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGTT  <  SRR4302547.5589441/94‑1 (MQ=60)
                                                                                            ACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGTT  <  SRR4302547.4089504/94‑1 (MQ=60)
                                                                                            ACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGTT  >  SRR4302547.337567/1‑94 (MQ=60)
                                                                                            |                                                                                             
CTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTTGCCCGATTACTGGGGACTTGCGGGGATCAGCAGTTCAAAGGTACCGGGTGTTGCGGGAATCGGACCAAAAAGCGCCACGCAGCTGCTGGTCGAGTTTCAGAGTCTGGAAGGGATATATGAGAATCTGGATGCGGTT  >  NC_000913/2929521‑2929706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑