Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,974,569 C→T intergenic (‑533/‑52) aas ← / → galR 2‑acyl‑glycerophospho‑ethanolamine acyltransferase/GalR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,974,5690CT85.5% 735.5 / 95.0 276intergenic (‑533/‑52)aas/galR2‑acyl‑glycerophospho‑ethanolamine acyltransferase/GalR
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base T (113/122);  total (135/141)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-04

Only 100 of 280 total aligned reads displayed.

CAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTT  >  NC_000913/2974479‑2974659
                                                                                          |                                                                                          
cAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGgtgt                                                                                          >  2:5677450/1‑93 (MQ=255)
   aGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGtttt                                                                                       >  1:5419057/1‑93 (MQ=255)
    ggTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTc                                                                                      >  1:2161623/1‑93 (MQ=255)
      tttGCAATCTCTTTTTTTGCCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCg                                                                                    >  1:6492797/1‑93 (MQ=255)
       ttGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGt                                                                                   <  2:6589417/93‑1 (MQ=255)
        tGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTa                                                                                  >  1:5601998/1‑93 (MQ=255)
         gCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTaa                                                                                 >  1:5124499/1‑93 (MQ=255)
          cAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAac                                                                                <  2:529764/93‑1 (MQ=255)
          cAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAac                                                                                >  2:5389929/1‑93 (MQ=255)
             tctcTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACt                                                                             >  2:4810209/1‑93 (MQ=255)
             tctcTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACt                                                                             >  1:5541029/1‑93 (MQ=255)
             tctcTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACt                                                                             >  2:1190335/1‑93 (MQ=255)
               tctTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGa                                                                           >  2:590439/1‑93 (MQ=255)
               tctTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGa                                                                           <  2:893936/93‑1 (MQ=255)
                ctTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGaa                                                                          >  2:1987708/1‑93 (MQ=255)
                 tttttttGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGaaa                                                                         <  2:4766890/93‑1 (MQ=255)
                  ttttttGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAg                                                                        <  1:2096477/93‑1 (MQ=255)
                    ttttGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGaa                                                                      <  2:5752091/93‑1 (MQ=255)
                    ttttGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGaa                                                                      <  1:699480/93‑1 (MQ=255)
                     tttGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAAt                                                                     <  1:1538887/93‑1 (MQ=255)
                      ttGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATg                                                                    >  1:5479560/1‑93 (MQ=255)
                      ttGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATg                                                                    >  2:2111172/1‑93 (MQ=255)
                       tGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGt                                                                   <  1:642726/93‑1 (MQ=255)
                       tGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGt                                                                   >  2:5040108/1‑93 (MQ=255)
                        gTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTa                                                                  >  2:3294444/1‑93 (MQ=255)
                         tCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTaa                                                                 >  2:620829/1‑93 (MQ=255)
                         tCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTaa                                                                 <  1:5938281/93‑1 (MQ=255)
                         tCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTaa                                                                 <  2:3476680/93‑1 (MQ=255)
                         tCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTaa                                                                 <  1:1842259/93‑1 (MQ=255)
                         tCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTaa                                                                 <  2:4103017/93‑1 (MQ=255)
                          cttacttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAg                                                                >  1:5372959/1‑93 (MQ=255)
                          cttacttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAg                                                                <  2:4630072/93‑1 (MQ=255)
                           ttacttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGc                                                               <  2:2242186/93‑1 (MQ=255)
                           ttacttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGc                                                               <  1:2587678/93‑1 (MQ=255)
                            tacttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCg                                                              <  2:1757976/93‑1 (MQ=255)
                             acttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGt                                                             >  1:3346313/1‑93 (MQ=255)
                              cttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGtt                                                            >  2:691113/1‑93 (MQ=255)
                               ttaTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGttt                                                           >  1:3146056/1‑93 (MQ=255)
                                taTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTa                                                          <  1:4645227/93‑1 (MQ=255)
                                taTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTa                                                          <  2:380421/93‑1 (MQ=255)
                                taTTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTa                                                          >  2:4305076/1‑93 (MQ=255)
                                  tttGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTAcc                                                        >  2:3255098/1‑93 (MQ=255)
                                  tttGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTAcc                                                        >  1:457547/1‑93 (MQ=255)
                                    tGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCa                                                      >  1:903727/1‑93 (MQ=255)
                                     gCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCAc                                                     <  2:5709047/93‑1 (MQ=255)
                                     gCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCAc                                                     <  2:649620/93‑1 (MQ=255)
                                        tttGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTaa                                                  <  1:5629617/93‑1 (MQ=255)
                                        tttGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTaa                                                  >  1:5209720/1‑93 (MQ=255)
                                           ggCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGt                                               <  1:1922674/93‑1 (MQ=255)
                                             cTCACATTACCACGATGAAAAAACGCCACCCCTTGAACCAACGGGAGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTAt                                             >  1:3226500/1‑93 (MQ=255)
                                               cacaTTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTAttt                                           >  1:2966862/1‑93 (MQ=255)
                                               cacaTTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTAttt                                           <  2:2609112/93‑1 (MQ=255)
                                                acaTTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTAtttt                                          >  2:4839159/1‑93 (MQ=255)
                                                 caTTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTc                                         >  2:1538319/1‑93 (MQ=255)
                                                 caTTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTc                                         >  2:5412761/1‑93 (MQ=255)
                                                  aTTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCa                                        <  1:3945530/93‑1 (MQ=255)
                                                  aTTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCa                                        <  1:2355967/93‑1 (MQ=255)
                                                   ttCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCAt                                       <  2:4850048/93‑1 (MQ=255)
                                                   ttCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCAt                                       >  1:6376841/1‑93 (MQ=255)
                                                    tCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATg                                      <  1:869862/93‑1 (MQ=255)
                                                     cccACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATgg                                     <  1:4173107/93‑1 (MQ=255)
                                                      ccACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGc                                    <  2:6524909/93‑1 (MQ=255)
                                                       cACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCg                                   >  2:6369237/1‑93 (MQ=255)
                                                         cGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGAc                                 >  1:2772630/1‑93 (MQ=255)
                                                          gATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGAcc                                <  2:6006849/93‑1 (MQ=255)
                                                           aTGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCa                               >  1:6201373/1‑93 (MQ=255)
                                                            tGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCAt                              >  1:3836195/1‑93 (MQ=255)
                                                             gAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATa                             <  1:4492643/93‑1 (MQ=255)
                                                               aaaCACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATaaa                           <  2:2457817/93‑1 (MQ=255)
                                                               aaaCACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATaaa                           <  2:2327104/93‑1 (MQ=255)
                                                                aaCACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAg                          >  1:6005951/1‑93 (MQ=255)
                                                                  cacGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGa                        >  2:2178690/1‑93 (MQ=255)
                                                                  cacGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGa                        <  1:4347942/93‑1 (MQ=255)
                                                                  cacGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGa                        <  2:2906067/93‑1 (MQ=255)
                                                                    cGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATg                      <  1:261675/93‑1 (MQ=255)
                                                                    cGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATg                      <  1:1647918/93‑1 (MQ=255)
                                                                    cGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATg                      >  1:138205/1‑93 (MQ=255)
                                                                        aCCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGc                  >  1:1012933/1‑93 (MQ=255)
                                                                         ccccTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGcc                 >  2:4019084/1‑93 (MQ=255)
                                                                         ccccTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGcc                 <  2:4336053/93‑1 (MQ=255)
                                                                          cccTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGccc                >  1:225316/1‑93 (MQ=255)
                                                                          cccTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGccc                <  1:5074852/93‑1 (MQ=255)
                                                                           ccTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCg               >  1:1086598/1‑93 (MQ=255)
                                                                           ccTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCg               <  2:3601809/93‑1 (MQ=255)
                                                                           ccTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCg               <  2:3061822/93‑1 (MQ=255)
                                                                            cTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGa              >  2:4399962/1‑93 (MQ=255)
                                                                            cTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGa              >  2:5124037/1‑93 (MQ=255)
                                                                            cTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGa              >  1:4756566/1‑93 (MQ=255)
                                                                            cTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGa              >  1:389873/1‑93 (MQ=255)
                                                                             ttGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGAc             <  1:1216860/93‑1 (MQ=255)
                                                                             ttGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGAc             <  2:4274288/93‑1 (MQ=255)
                                                                              tGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACt            <  1:4221703/93‑1 (MQ=255)
                                                                               gAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTg           <  1:3650420/93‑1 (MQ=255)
                                                                                 aCCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGc         <  1:2269214/93‑1 (MQ=255)
                                                                                  ccAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCa        >  1:980663/1‑93 (MQ=255)
                                                                                  ccAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCa        <  1:5848606/93‑1 (MQ=255)
                                                                                      cGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCg    <  2:1908293/93‑1 (MQ=255)
                                                                                      cGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCg    <  2:2302494/93‑1 (MQ=255)
                                                                                       gggTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGt   >  2:642190/1‑93 (MQ=255)
                                                                                       gggTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGt   >  2:4194091/1‑93 (MQ=255)
                                                                                          |                                                                                          
CAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTT  >  NC_000913/2974479‑2974659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCA  >  NC_000913/2974476‑2974662
                                                                                             |                                                                                             
AACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGT                                                                                               <  SRR4302547.4952980/94‑1 (MQ=60)
AACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGT                                                                                               >  SRR4302547.877109/1‑94 (MQ=60)
AACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGT                                                                                               >  SRR4302547.1261628/1‑94 (MQ=60)
  CCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGT                                                                                             >  SRR4302547.2396509/1‑94 (MQ=60)
  CCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGT                                                                                             <  SRR4302547.5666701/94‑1 (MQ=60)
    AAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTT                                                                                           <  SRR4302547.4048950/94‑1 (MQ=60)
     AAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTT                                                                                          >  SRR4302547.5531147/1‑94 (MQ=60)
      AGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTC                                                                                         >  SRR4302547.2204826/1‑94 (MQ=60)
        GTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCG                                                                                       <  SRR4302547.6519515/94‑1 (MQ=60)
        GTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCG                                                                                       >  SRR4302547.2269043/1‑94 (MQ=60)
        GTTTGCAATCTCTTTTTTTGCCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCG                                                                                       >  SRR4302547.6627966/1‑94 (MQ=60)
         TTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGT                                                                                      <  SRR4302547.4386044/94‑1 (MQ=60)
          TTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTA                                                                                     >  SRR4302547.5717565/1‑94 (MQ=60)
           TGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAA                                                                                    >  SRR4302547.5230092/1‑94 (MQ=60)
           TGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAA                                                                                    >  SRR4302547.2693600/1‑94 (MQ=60)
            GCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAAC                                                                                   <  SRR4302547.1674394/94‑1 (MQ=60)
            GCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAAC                                                                                   <  SRR4302547.1226203/94‑1 (MQ=60)
             CAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACA                                                                                  >  SRR4302547.2313306/1‑94 (MQ=60)
             CAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACA                                                                                  >  SRR4302547.5672424/1‑94 (MQ=60)
               ATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACT                                                                                >  SRR4302547.5950890/1‑94 (MQ=60)
               ATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACT                                                                                <  SRR4302547.2642130/94‑1 (MQ=60)
               ATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACT                                                                                >  SRR4302547.5655387/1‑94 (MQ=60)
               ATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACT                                                                                <  SRR4302547.6767223/94‑1 (MQ=60)
                TCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTG                                                                               <  SRR4302547.4829684/94‑1 (MQ=60)
                 CTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGA                                                                              <  SRR4302547.887194/94‑1 (MQ=60)
                 CTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGA                                                                              >  SRR4302547.4661311/1‑94 (MQ=60)
                  TCTTTTTTTGTCTTTCTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACCGGGGTTTTCCGTTACACCGAA                                                                             >  SRR4302547.1795884/1‑94 (MQ=60)
                     TTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGA                                                                          <  SRR4302547.2138273/94‑1 (MQ=60)
                       TTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAAT                                                                        <  SRR4302547.712404/94‑1 (MQ=60)
                        TTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATG                                                                       <  SRR4302547.1569522/94‑1 (MQ=60)
                        TTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATG                                                                       <  SRR4302547.2539163/94‑1 (MQ=60)
                        TTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATG                                                                       >  SRR4302547.5592710/1‑94 (MQ=60)
                          TGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTA                                                                     >  SRR4302547.1271216/1‑94 (MQ=60)
                          TGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTA                                                                     <  SRR4302547.5634222/94‑1 (MQ=60)
                          TGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTA                                                                     >  SRR4302547.5253315/1‑94 (MQ=60)
                          TGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTA                                                                     <  SRR4302547.654579/94‑1 (MQ=60)
                          TGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTA                                                                     >  SRR4302547.5728457/1‑94 (MQ=60)
                            TCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAG                                                                   <  SRR4302547.1596894/94‑1 (MQ=60)
                            TCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAG                                                                   <  SRR4302547.5583173/94‑1 (MQ=60)
                            TCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAG                                                                   <  SRR4302547.1878662/94‑1 (MQ=60)
                            TCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAG                                                                   <  SRR4302547.6061417/94‑1 (MQ=60)
                            TCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAG                                                                   >  SRR4302547.5483657/1‑94 (MQ=60)
                            TCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAG                                                                   >  SRR4302547.5483840/1‑94 (MQ=60)
                            TCTTACTTATTTGCGTTTGGCCCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAG                                                                   <  SRR4302547.5238336/94‑1 (MQ=60)
                             CTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGC                                                                  >  SRR4302547.4548520/1‑94 (MQ=60)
                              TTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCG                                                                 <  SRR4302547.2805455/94‑1 (MQ=60)
                              TTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCG                                                                 <  SRR4302547.2639891/94‑1 (MQ=60)
                               TACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGT                                                                >  SRR4302547.3414106/1‑94 (MQ=60)
                                ACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTT                                                               >  SRR4302547.1942470/1‑94 (MQ=60)
                                 CTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTT                                                              >  SRR4302547.3208567/1‑94 (MQ=60)
                                  TTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTA                                                             <  SRR4302547.6111368/94‑1 (MQ=60)
                                   TATTTGCGTTTTGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTAC                                                            >  SRR4302547.364322/1‑94 (MQ=60)
                                   TATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTAC                                                            <  SRR4302547.4741423/94‑1 (MQ=60)
                                    ATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACC                                                           >  SRR4302547.466011/1‑94 (MQ=60)
                                    ATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACC                                                           >  SRR4302547.5743132/1‑94 (MQ=60)
                                    ATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACC                                                           <  SRR4302547.5504642/94‑1 (MQ=60)
                                    ATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACC                                                           <  SRR4302547.3212293/94‑1 (MQ=60)
                                     TTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCC                                                          <  SRR4302547.3309038/94‑1 (MQ=60)
                                     TTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCC                                                          <  SRR4302547.5601416/94‑1 (MQ=60)
                                      TTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCA                                                         >  SRR4302547.921128/1‑94 (MQ=60)
                                      TTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCA                                                         <  SRR4302547.5876236/94‑1 (MQ=60)
                                      TTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCA                                                         <  SRR4302547.3310825/94‑1 (MQ=60)
                                       TGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCAC                                                        >  SRR4302547.5674991/1‑94 (MQ=60)
                                        GCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACT                                                       <  SRR4302547.3775765/94‑1 (MQ=60)
                                         CGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTA                                                      >  SRR4302547.4262827/1‑94 (MQ=60)
                                          GTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAA                                                     >  SRR4302547.5316932/1‑94 (MQ=60)
                                           TTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAG                                                    <  SRR4302547.5745801/94‑1 (MQ=60)
                                             TGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGT                                                  >  SRR4302547.3950853/1‑94 (MQ=60)
                                              TGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTA                                                 <  SRR4302547.6143230/94‑1 (MQ=60)
                                              GGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTA                                                 <  SRR4302547.1960745/94‑1 (MQ=60)
                                              GGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTA                                                 >  SRR4302547.3609547/1‑94 (MQ=60)
                                               GCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTAT                                                >  SRR4302547.198544/1‑94 (MQ=60)
                                               GCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTAT                                                <  SRR4302547.1806210/94‑1 (MQ=60)
                                               GCTCACATTACCACGATGAAAAAACGCCACCCCTTGAACCAACGGGAGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTAT                                                >  SRR4302547.3290895/1‑94 (MQ=60)
                                                CTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATT                                               >  SRR4302547.1086102/1‑94 (MQ=60)
                                                 TCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTT                                              >  SRR4302547.3025909/1‑94 (MQ=60)
                                                 TCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTT                                              <  SRR4302547.2224466/94‑1 (MQ=60)
                                                  CACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTT                                             <  SRR4302547.2652973/94‑1 (MQ=60)
                                                  CACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTT                                             >  SRR4302547.773099/1‑94 (MQ=60)
                                                    CATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCA                                           <  SRR4302547.970020/94‑1 (MQ=60)
                                                     ATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCAT                                          <  SRR4302547.2403708/94‑1 (MQ=60)
                                                     ATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCAT                                          <  SRR4302547.4025774/94‑1 (MQ=60)
                                                     ATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCAT                                          >  SRR4302547.6509905/1‑94 (MQ=60)
                                                     ATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCAT                                          <  SRR4302547.1504795/94‑1 (MQ=60)
                                                     ATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGCGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCAT                                          <  SRR4302547.6324760/94‑1 (MQ=60)
                                                       TCCCACGATGAAAACACGCCTCCCCTTGAACCAACGTGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGG                                        <  SRR4302547.4993533/94‑1 (MQ=60)
                                                       TCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGG                                        <  SRR4302547.4499219/94‑1 (MQ=60)
                                                       TCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGG                                        <  SRR4302547.886532/94‑1 (MQ=60)
                                                        CCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGC                                       <  SRR4302547.4258119/94‑1 (MQ=60)
                                                        CCCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGC                                       <  SRR4302547.194835/94‑1 (MQ=60)
                                                         CCACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCG                                      <  SRR4302547.2335051/94‑1 (MQ=60)
                                                          CACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGA                                     <  SRR4302547.2565971/94‑1 (MQ=60)
                                                          CACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGA                                     <  SRR4302547.1232328/94‑1 (MQ=60)
                                                           ACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGAC                                    >  SRR4302547.2828174/1‑94 (MQ=60)
                                                           ACGATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGAC                                    >  SRR4302547.2085010/1‑94 (MQ=60)
                                                             GATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCA                                  >  SRR4302547.6330127/1‑94 (MQ=60)
                                                              CTGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCAT                                 >  SRR4302547.3914334/1‑94 (MQ=60)
                                                              ATGAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCAT                                 >  SRR4302547.2178017/1‑94 (MQ=60)
                                                                GAAAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAA                               >  SRR4302547.4745876/1‑94 (MQ=60)
                                                                GAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAA                               <  SRR4302547.4585443/94‑1 (MQ=60)
                                                                  AAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAG                             >  SRR4302547.6130591/1‑94 (MQ=60)
                                                                  AAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAG                             >  SRR4302547.6341630/1‑94 (MQ=60)
                                                                  AAACACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAG                             <  SRR4302547.5398943/94‑1 (MQ=60)
                                                                  AAACACGCAACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATGAAG                             >  SRR4302547.2151085/1‑94 (MQ=60)
                                                                     CACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGAT                          >  SRR4302547.988679/1‑94 (MQ=60)
                                                                     CACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGAT                          <  SRR4302547.4437297/94‑1 (MQ=60)
                                                                      ACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATG                         >  SRR4302547.140703/1‑94 (MQ=60)
                                                                      ACGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATG                         <  SRR4302547.3978447/94‑1 (MQ=60)
                                                                       CGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGT                        >  SRR4302547.2849688/1‑94 (MQ=60)
                                                                       CGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGT                        <  SRR4302547.3172152/94‑1 (MQ=60)
                                                                       CGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGT                        <  SRR4302547.1680458/94‑1 (MQ=60)
                                                                       CGCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGT                        <  SRR4302547.266564/94‑1 (MQ=60)
                                                                        GCCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTGCCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTA                       >  SRR4302547.829671/1‑94 (MQ=60)
                                                                         CCACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAG                      >  SRR4302547.4739455/1‑94 (MQ=60)
                                                                          CACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGC                     >  SRR4302547.3945089/1‑94 (MQ=60)
                                                                          CACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGC                     <  SRR4302547.4281826/94‑1 (MQ=60)
                                                                          CACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGC                     >  SRR4302547.1442146/1‑94 (MQ=60)
                                                                          CACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGC                     <  SRR4302547.1264375/94‑1 (MQ=60)
                                                                          CACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGC                     >  SRR4302547.1032733/1‑94 (MQ=60)
                                                                           ACCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCC                    >  SRR4302547.5181488/1‑94 (MQ=60)
                                                                            CCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCC                   <  SRR4302547.660823/94‑1 (MQ=60)
                                                                            CCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCC                   >  SRR4302547.638811/1‑94 (MQ=60)
                                                                            CCCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCC                   >  SRR4302547.229546/1‑94 (MQ=60)
                                                                             CCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCG                  >  SRR4302547.1108319/1‑94 (MQ=60)
                                                                             CCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCG                  <  SRR4302547.5179547/94‑1 (MQ=60)
                                                                             CCCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCACG                  >  SRR4302547.6006359/1‑94 (MQ=60)
                                                                              CCTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGA                 >  SRR4302547.4855123/1‑94 (MQ=60)
                                                                              CCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGA                 >  SRR4302547.4016167/1‑94 (MQ=60)
                                                                              CCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGA                 >  SRR4302547.397104/1‑94 (MQ=60)
                                                                               CTTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGAC                >  SRR4302547.4033676/1‑94 (MQ=60)
                                                                                TTGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACT               <  SRR4302547.1241427/94‑1 (MQ=60)
                                                                                 TGAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTG              <  SRR4302547.4307929/94‑1 (MQ=60)
                                                                                  GAAGCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGGCCATAAAGGATGTAGCCCGACTGG             <  SRR4302547.469551/94‑1 (MQ=60)
                                                                                  GAACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGG             <  SRR4302547.3724877/94‑1 (MQ=60)
                                                                                   AACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGC            >  SRR4302547.5218550/1‑94 (MQ=60)
                                                                                    ACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCA           >  SRR4302547.999748/1‑94 (MQ=60)
                                                                                    ACCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCA           <  SRR4302547.2315104/94‑1 (MQ=60)
                                                                                     CCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAG          <  SRR4302547.6525491/94‑1 (MQ=60)
                                                                                     CCAACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAG          <  SRR4302547.5969579/94‑1 (MQ=60)
                                                                                       AACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGC        <  SRR4302547.6106318/94‑1 (MQ=60)
                                                                                        ACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCG       <  SRR4302547.3239889/94‑1 (MQ=60)
                                                                                        ACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCG       >  SRR4302547.549114/1‑94 (MQ=60)
                                                                                        ACGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCAACTGGCAGGCG       <  SRR4302547.3063431/94‑1 (MQ=60)
                                                                                         CGGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGT      <  SRR4302547.4625786/94‑1 (MQ=60)
                                                                                          GGGTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTT     >  SRR4302547.4547293/1‑94 (MQ=60)
                                                                                          GGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTT     >  SRR4302547.908265/1‑94 (MQ=60)
                                                                                            GTGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTC   <  SRR4302547.338118/94‑1 (MQ=60)
                                                                                             TGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCA  <  SRR4302547.687396/94‑1 (MQ=60)
                                                                                             CGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCA  >  SRR4302547.1186751/1‑94 (MQ=60)
                                                                                             |                                                                                             
AACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAAACACGCCACCCCTTGAACCAACGGGCGTTTTCCGTAACACTGAAAGAATGTAAGCGTTTACCCACTAAGGTATTTTCATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCA  >  NC_000913/2974476‑2974662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑