Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,977,924 A→G L294L (TTA→TTG)  lysR → LysR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,977,9240AG99.1% 733.3 / ‑2.9 212L294L (TTA→TTG) lysRLysR
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (95/115);  total (97/115)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 218 total aligned reads displayed.

GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013
                                                                                          |                                                                                         
ggTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGcc                                                                                         <  2:5410004/93‑1 (MQ=255)
 gTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCg                                                                                        >  1:4391234/1‑93 (MQ=255)
    ccGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaaa                                                                                     <  1:5027741/93‑1 (MQ=255)
    ccGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaaa                                                                                     <  2:3255452/93‑1 (MQ=255)
     cGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAc                                                                                    <  1:2522930/93‑1 (MQ=255)
     cGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAc                                                                                    <  2:280239/93‑1 (MQ=255)
      gTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACt                                                                                   >  2:4442318/1‑93 (MQ=255)
       ttCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  >  1:2228246/1‑93 (MQ=255)
        tCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTgg                                                                                 >  2:3707963/1‑93 (MQ=255)
         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                <  2:5773554/93‑1 (MQ=255)
         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                <  2:5749659/93‑1 (MQ=255)
         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                <  2:1812918/93‑1 (MQ=255)
         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                >  2:4067689/1‑93 (MQ=255)
          aCCGTCAGCGTGATCCTCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTc                                                                               <  1:2632049/93‑1 (MQ=255)
           ccGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCa                                                                              <  2:6137138/93‑1 (MQ=255)
             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            <  2:3921531/93‑1 (MQ=255)
             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            <  1:4560604/93‑1 (MQ=255)
             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            >  1:5878218/1‑93 (MQ=255)
             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            >  2:700397/1‑93 (MQ=255)
              tCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACtt                                                                           <  1:3684598/93‑1 (MQ=255)
               cAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtc                                                                          <  1:2575082/93‑1 (MQ=255)
                 gCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtctc                                                                        >  1:3326137/1‑93 (MQ=255)
                 gCCTGATCCGCCCCCTGAACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtctc                                                                        >  2:4418429/1‑93 (MQ=255)
                  ccTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTctct                                                                       <  1:3672930/93‑1 (MQ=255)
                  ccTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTctct                                                                       >  1:2182229/1‑93 (MQ=255)
                   cTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCtt                                                                      <  2:3475899/93‑1 (MQ=255)
                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  2:330219/1‑93 (MQ=255)
                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  2:2619478/1‑93 (MQ=255)
                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  1:4355521/1‑93 (MQ=255)
                     gATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGa                                                                    >  2:3987999/1‑93 (MQ=255)
                        tcGCCCCCTTCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGTGTTGCCGAAACTGGTCACTTCTCTTGACGc                                                                 <  2:2058334/92‑1 (MQ=255)
                        ccGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGc                                                                 >  1:4428413/1‑93 (MQ=255)
                         cGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCt                                                                >  1:471018/1‑93 (MQ=255)
                           cccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAt                                                              >  1:5268031/1‑93 (MQ=255)
                           cccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAAGCTAt                                                              >  1:1475276/1‑93 (MQ=255)
                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            <  1:3770001/93‑1 (MQ=255)
                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            >  2:1674900/1‑93 (MQ=255)
                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           <  2:4003852/93‑1 (MQ=255)
                               cTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTg                                                          >  2:2379035/1‑93 (MQ=255)
                               cTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTg                                                          <  1:739550/93‑1 (MQ=255)
                                  cACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcg                                                       >  2:2770802/1‑93 (MQ=255)
                                     cGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCa                                                    <  2:1657343/93‑1 (MQ=255)
                                      gCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAg                                                   <  2:4578961/93‑1 (MQ=255)
                                       cccGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGc                                                  >  1:2784433/1‑93 (MQ=255)
                                         cGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTa                                                >  1:4772268/1‑93 (MQ=255)
                                         cGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTa                                                <  1:1845179/93‑1 (MQ=255)
                                         cGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTa                                                <  2:5162164/93‑1 (MQ=255)
                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               <  2:5977153/93‑1 (MQ=255)
                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               <  2:5935705/93‑1 (MQ=255)
                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCGAGTTAGTCGTCAGCTAc                                               >  1:5485252/1‑93 (MQ=255)
                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGCTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               <  2:2871678/93‑1 (MQ=255)
                                          gTCATCAGCGCTGGTCCAGGGGTTTAGGGGGAATTTAAAACGGGGGTGGCCGAAATGGGTCACTTTCCTTGCCGCATTTTGGTGGTCGGCTAc                                               >  2:1725124/1‑93 (MQ=255)
                                           tcatcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTTGTCAGCTACg                                              >  1:1563956/1‑93 (MQ=255)
                                           tcatcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACg                                              <  1:2104856/93‑1 (MQ=255)
                                            catcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGa                                             >  2:5447148/1‑93 (MQ=255)
                                            catcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGa                                             <  2:3614504/93‑1 (MQ=255)
                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  2:5135613/1‑93 (MQ=255)
                                              tcaGCGCTGGTTCAGGCGTTTATTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  2:1494715/93‑1 (MQ=255)
                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           >  2:1706645/1‑93 (MQ=255)
                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  2:1252013/93‑1 (MQ=255)
                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           >  1:3564210/1‑93 (MQ=255)
                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  1:4822052/93‑1 (MQ=255)
                                                aGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGc                                         <  2:2706597/93‑1 (MQ=255)
                                                aGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGc                                         <  2:3059187/93‑1 (MQ=255)
                                                 gcgcTGGTTCAGGCGTTTAGCGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        >  1:3062361/1‑93 (MQ=255)
                                                  cgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAt                                       >  2:4347217/1‑93 (MQ=255)
                                                    cTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaa                                     >  2:4609433/1‑93 (MQ=255)
                                                    cTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaa                                     <  1:5975326/93‑1 (MQ=255)
                                                     tGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaa                                    >  1:1709833/1‑93 (MQ=255)
                                                     tGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGCTGCCGAAACTGGCCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACGGCATaaa                                    >  1:2005604/1‑93 (MQ=255)
                                                      ggTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaaa                                   <  2:3840490/93‑1 (MQ=255)
                                                        ttCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGc                                 <  1:3156148/93‑1 (MQ=255)
                                                            ggCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACa                             <  1:5344105/93‑1 (MQ=255)
                                                            ggCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACa                             <  2:3145824/93‑1 (MQ=255)
                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  1:625582/93‑1 (MQ=255)
                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         <  1:5516969/93‑1 (MQ=255)
                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         >  2:384528/1‑93 (MQ=255)
                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         <  2:2813378/93‑1 (MQ=255)
                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  2:920069/93‑1 (MQ=255)
                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  1:3987870/93‑1 (MQ=255)
                                                                     tGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCg                    >  2:1363452/1‑93 (MQ=255)
                                                                      gggCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCgg                   >  2:1334591/1‑93 (MQ=255)
                                                                      gggCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCgg                   <  1:5638859/93‑1 (MQ=255)
                                                                       ggCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGc                  <  2:3518966/93‑1 (MQ=255)
                                                                        gCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCa                 >  1:6056274/1‑93 (MQ=255)
                                                                         cATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCAt                <  2:2070841/93‑1 (MQ=255)
                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              <  2:570138/93‑1 (MQ=255)
                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  2:4911232/1‑93 (MQ=255)
                                                                            ttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGa             <  1:1270510/93‑1 (MQ=255)
                                                                             tACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt            <  1:5053903/93‑1 (MQ=255)
                                                                             tACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt            <  2:5852613/93‑1 (MQ=255)
                                                                              tcAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGGCGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATc           <  2:838334/92‑1 (MQ=255)
                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:1143124/1‑93 (MQ=255)
                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:1106409/1‑93 (MQ=255)
                                                                                aaGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgc         <  1:638005/93‑1 (MQ=255)
                                                                                  gCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccg       <  1:856109/93‑1 (MQ=255)
                                                                                   gggggTTGCCAAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccgc      <  1:2435441/92‑1 (MQ=255)
                                                                                     gggTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCgg    <  1:1851442/93‑1 (MQ=255)
                                                                                       gTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTa  <  2:117308/93‑1 (MQ=255)
                                                                                       gTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTa  >  2:773004/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAA  >  NC_000913/2977833‑2978017
                                                                                           |                                                                                             
CGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCC                                                                                             <  SRR4302532.2672007/94‑1 (MQ=60)
CGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCC                                                                                             <  SRR4302532.6238525/94‑1 (MQ=60)
 GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCG                                                                                            >  SRR4302532.4540551/1‑94 (MQ=60)
     CCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAC                                                                                        <  SRR4302532.5200192/94‑1 (MQ=60)
      CGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACT                                                                                       >  SRR4302532.4859955/1‑94 (MQ=60)
      CGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACT                                                                                       <  SRR4302532.2607121/94‑1 (MQ=60)
      CGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGGTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACT                                                                                       >  SRR4302532.3283124/1‑94 (MQ=60)
       GTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTG                                                                                      >  SRR4302532.4238584/1‑94 (MQ=60)
       GTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTG                                                                                      >  SRR4302532.2302187/1‑94 (MQ=60)
           ACCGTCAGCGTGATCCTCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCA                                                                                  <  SRR4302532.2720239/94‑1 (MQ=60)
             CGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACT                                                                                >  SRR4302532.2795137/1‑94 (MQ=60)
             CGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACT                                                                                >  SRR4302532.1253014/1‑94 (MQ=60)
             CGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACT                                                                                <  SRR4302532.3178616/94‑1 (MQ=60)
             CGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACT                                                                                <  SRR4302532.5358780/94‑1 (MQ=60)
             CGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACT                                                                                >  SRR4302532.6081840/1‑94 (MQ=60)
              GTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTT                                                                               <  SRR4302532.4716176/94‑1 (MQ=60)
               TCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTC                                                                              <  SRR4302532.3808336/94‑1 (MQ=60)
                CAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCT                                                                             <  SRR4302532.2661106/94‑1 (MQ=60)
                 AGCCTGATCCGCCTCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTC                                                                            >  SRR4302532.5218463/1‑94 (MQ=60)
                 AGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTC                                                                            >  SRR4302532.3437055/1‑94 (MQ=60)
                  GCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCT                                                                           >  SRR4302532.2255044/1‑94 (MQ=60)
                  GCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCT                                                                           <  SRR4302532.4578671/94‑1 (MQ=60)
                   CCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTT                                                                          <  SRR4302532.3796316/94‑1 (MQ=60)
                    CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         <  SRR4302532.201200/94‑1 (MQ=60)
                    CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         >  SRR4302532.4502981/1‑94 (MQ=60)
                    CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         <  SRR4302532.1413230/94‑1 (MQ=60)
                    CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         >  SRR4302532.4977215/1‑94 (MQ=60)
                     TGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGA                                                                        >  SRR4302532.1840626/1‑94 (MQ=60)
                      catccgccccctgcccattcctTCATCAGCGCTGGTTCAGGCCTTTAGCGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCGCTTCTCTTGAC                                                                       <  SRR4302532.1913231/72‑1 (MQ=60)
                       ATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACG                                                                      >  SRR4302532.1793442/1‑94 (MQ=60)
                        TCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGC                                                                     >  SRR4302532.4579281/1‑94 (MQ=60)
                         CCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCT                                                                    >  SRR4302532.487420/1‑94 (MQ=60)
                         CCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCT                                                                    >  SRR4302532.4668516/1‑94 (MQ=60)
                         CCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCT                                                                    <  SRR4302532.5737890/94‑1 (MQ=60)
                         CCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCT                                                                    <  SRR4302532.5982266/94‑1 (MQ=60)
                           GCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAT                                                                  <  SRR4302532.6206140/94‑1 (MQ=60)
                           GCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAT                                                                  >  SRR4302532.5449688/1‑94 (MQ=60)
                           GCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAAGCTAT                                                                  >  SRR4302532.1524980/1‑94 (MQ=60)
                             CCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTT                                                                >  SRR4302532.2708078/1‑94 (MQ=60)
                              CCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTT                                                               <  SRR4302532.3896883/94‑1 (MQ=60)
                               CCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTG                                                              <  SRR4302532.6067206/94‑1 (MQ=60)
                                CTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGT                                                             <  SRR4302532.764761/94‑1 (MQ=60)
                                CTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGT                                                             >  SRR4302532.3927844/1‑94 (MQ=60)
                                CTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGT                                                             <  SRR4302532.3053939/94‑1 (MQ=60)
                                  GCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCG                                                           <  SRR4302532.312860/94‑1 (MQ=60)
                                    ACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTC                                                         >  SRR4302532.490280/1‑94 (MQ=60)
                                    ACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTC                                                         <  SRR4302532.3491792/94‑1 (MQ=60)
                                    ACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTC                                                         <  SRR4302532.5021553/94‑1 (MQ=60)
                                     CCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCA                                                        <  SRR4302532.3804966/94‑1 (MQ=60)
                                      CGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAG                                                       >  SRR4302532.5606025/1‑94 (MQ=60)
                                       GCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGC                                                      >  SRR4302532.2877835/1‑94 (MQ=60)
                                         CCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTA                                                    >  SRR4302532.4936472/1‑94 (MQ=60)
                                          CGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAC                                                   <  SRR4302532.1906441/94‑1 (MQ=60)
                                          CGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCGAGTTAGTCGTCAGCTAC                                                   >  SRR4302532.5675439/1‑94 (MQ=60)
                                           GTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTTGTCAGCTACG                                                  >  SRR4302532.1616853/1‑94 (MQ=60)
                                            TCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTATGA                                                 >  SRR4302532.3705550/1‑94 (MQ=60)
                                            TCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGA                                                 <  SRR4302532.2175106/94‑1 (MQ=60)
                                            TCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGA                                                 <  SRR4302532.2175107/94‑1 (MQ=60)
                                             CATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAC                                                >  SRR4302532.1908178/1‑94 (MQ=60)
                                              atcaggcgtgtTTCAGGCGTTTAGTGGTCATTTACAAGCGGGGGTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACA                                               <  SRR4302532.3745484/83‑1 (MQ=60)
                                              ATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACA                                               >  SRR4302532.3683462/1‑94 (MQ=60)
                                               TCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAG                                              <  SRR4302532.4988051/94‑1 (MQ=60)
                                                CAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGC                                             >  SRR4302532.1706284/1‑94 (MQ=60)
                                                 AGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCA                                            <  SRR4302532.1171866/94‑1 (MQ=60)
                                                 AGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCA                                            >  SRR4302532.819367/1‑94 (MQ=60)
                                                 AGCGCTGGTTCAGGCGTTTAGCGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCA                                            >  SRR4302532.3163494/1‑94 (MQ=60)
                                                  GCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGGCAGCAT                                           >  SRR4302532.2572657/1‑94 (MQ=60)
                                                  GCGCTGGTTCAGGCGTGTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAT                                           <  SRR4302532.653120/94‑1 (MQ=60)
                                                     CTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAA                                        <  SRR4302532.6181867/94‑1 (MQ=60)
                                                     CTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAA                                        >  SRR4302532.1767474/1‑94 (MQ=60)
                                                     CTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGCTGCCGAAACTGGCCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACGGCATAAA                                        >  SRR4302532.2072482/1‑94 (MQ=60)
                                                     CTGGTTCAGGCGTTTAGTGGGCATTTACAAGAGGGGTTGCCGAAACTGGTAACTTCTCTTGAAGCTATTTTGTCGTCAGCTACGACAGCAGAAA                                        >  SRR4302532.4942923/1‑94 (MQ=60)
                                                         TTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCG                                    <  SRR4302532.3260906/94‑1 (MQ=60)
                                                         TTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCG                                    <  SRR4302532.232206/94‑1 (MQ=60)
                                                            AGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACA                                 <  SRR4302532.5332635/94‑1 (MQ=60)
                                                             GGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAG                                <  SRR4302532.4592723/94‑1 (MQ=60)
                                                             GGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAG                                <  SRR4302532.5529513/94‑1 (MQ=60)
                                                               CGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCA                              <  SRR4302532.647439/94‑1 (MQ=60)
                                                                GTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAT                             <  SRR4302532.2630028/94‑1 (MQ=60)
                                                                 TTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATC                            <  SRR4302532.5708409/94‑1 (MQ=60)
                                                                  TTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCC                           <  SRR4302532.1614328/94‑1 (MQ=60)
                                                                  TTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCA                           <  SRR4302532.4122198/94‑1 (MQ=60)
                                                                    AGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTC                         <  SRR4302532.6120382/94‑1 (MQ=60)
                                                                       GGGCATTTACAAGCGGGGTTGCCGAAACTGGTGACTTCTCTTGACGCTCTTTTGTCGTCAGCTACGACAGGATAAAAGCGACAGGATCCTCGta                      >  SRR4302532.676591/1‑92 (MQ=60)
                                                                       GGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGC                      <  SRR4302532.5834810/94‑1 (MQ=60)
                                                                        GGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCA                     <  SRR4302532.2159643/94‑1 (MQ=60)
                                                                        GGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCA                     <  SRR4302532.2267230/94‑1 (MQ=60)
                                                                        GGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCA                     >  SRR4302532.6265401/1‑94 (MQ=60)
                                                                         GCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCAT                    >  SRR4302532.3873235/1‑94 (MQ=60)
                                                                          CATTTATAAGCTGGGTTGTCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAATCGATAGTATCCTCGGCATG                   >  SRR4302532.5685196/1‑94 (MQ=60)
                                                                           ATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGG                  <  SRR4302532.86815/94‑1 (MQ=60)
                                                                           ATTTACAACCGGGGTTACCGAAACTGGGCACTTGTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCGCGGCATGG                  >  SRR4302532.2354022/1‑94 (MQ=60)
                                                                             TTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAT                <  SRR4302532.1313011/94‑1 (MQ=60)
                                                                              TACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATC               <  SRR4302532.5227358/94‑1 (MQ=60)
                                                                              TACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATC               <  SRR4302532.4514807/94‑1 (MQ=60)
                                                                               ACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCG              >  SRR4302532.1143398/1‑94 (MQ=60)
                                                                               ACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCG              <  SRR4302532.3821265/94‑1 (MQ=60)
                                                                               ACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCG              >  SRR4302532.1181259/1‑94 (MQ=60)
                                                                                 AAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCC            <  SRR4302532.660229/94‑1 (MQ=60)
                                                                                   GCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGC          <  SRR4302532.885526/94‑1 (MQ=60)
                                                                                   GCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGC          <  SRR4302532.6283474/94‑1 (MQ=60)
                                                                                    GGGGGTTGCCAAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCG         <  SRR4302532.2516415/94‑1 (MQ=60)
                                                                                    CGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCG         <  SRR4302532.5498287/94‑1 (MQ=60)
                                                                                      GGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGT       <  SRR4302532.1912878/94‑1 (MQ=60)
                                                                                        GTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTAT     >  SRR4302532.5733273/1‑94 (MQ=60)
                                                                                           GCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAA  <  SRR4302532.1882372/94‑1 (MQ=60)
                                                                                           |                                                                                             
CGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAA  >  NC_000913/2977833‑2978017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑