Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,924	A→G	L294L (TTA→TTG)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,924	0	A	G	100.0%	864.7 / NA	241	L294L (TTA→TTG)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (116/125); total (116/125)

Only 100 of 247 total aligned reads displayed.

GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013

 gTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCg                                                                                        >  2:2332771/1‑93 (MQ=255)

  ttCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGCTGCCGa                                                                                       >  2:5072564/1‑93 (MQ=255)

   tCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaa                                                                                      >  2:2201227/1‑93 (MQ=255)

   tCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaa                                                                                      <  2:2425182/93‑1 (MQ=255)

    tcGTTCACCGTCAGCCTGATCCTCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGaaa                                                                                     <  2:3009497/92‑1 (MQ=255)

     cGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAc                                                                                    >  2:1081583/1‑93 (MQ=255)

      gTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACt                                                                                   <  1:846414/93‑1 (MQ=255)

       ttCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  >  2:2583897/1‑93 (MQ=255)

       ttCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  <  2:3651215/93‑1 (MQ=255)

       tgcACCGTCAGCCTGATCCGCCCCCTGCCCCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTg                                                                                  <  1:2156798/91‑1 (MQ=255)

        tCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTgg                                                                                 <  2:4461666/93‑1 (MQ=255)

         cACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGt                                                                                >  1:6240241/1‑93 (MQ=255)

          aCCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTc                                                                               <  2:754883/93‑1 (MQ=255)

             gTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACt                                                                            <  1:285806/93‑1 (MQ=255)

              tCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACtt                                                                           <  2:451746/93‑1 (MQ=255)

               cAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtc                                                                          <  2:3780090/93‑1 (MQ=255)

               cAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTtc                                                                          <  1:3286876/93‑1 (MQ=255)

                  ccTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTctct                                                                       <  2:6392046/93‑1 (MQ=255)

                   cTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCtt                                                                      <  1:5554553/93‑1 (MQ=255)

                   cTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCtt                                                                      >  2:3409105/1‑93 (MQ=255)

                    tGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  1:835226/1‑93 (MQ=255)

                    tGATCCGCCCCCTACACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTg                                                                     >  2:3794248/1‑93 (MQ=255)

                     gATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGa                                                                    <  1:6361732/93‑1 (MQ=255)

                      aTCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAc                                                                   >  2:1181197/1‑93 (MQ=255)

                      aTCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAc                                                                   >  1:3615802/1‑93 (MQ=255)

                       tCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACg                                                                  <  1:2069928/93‑1 (MQ=255)

                       tCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACg                                                                  <  2:2807344/93‑1 (MQ=255)

                          gCCCCCTGCACCTCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTa                                                               <  1:116055/93‑1 (MQ=255)

                          gCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTa                                                               >  2:1452317/1‑93 (MQ=255)

                           cccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAt                                                              >  2:4988410/1‑93 (MQ=255)

                            ccccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtt                                                             >  1:6065633/1‑93 (MQ=255)

                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            <  2:4591113/93‑1 (MQ=255)

                             cccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAttt                                                            <  1:56837/93‑1 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           <  2:5653158/93‑1 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           >  1:6420039/1‑93 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           <  1:4681055/93‑1 (MQ=255)

                              ccTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAtttt                                                           >  2:4926510/1‑93 (MQ=255)

                               cTGCACCGCCCGTCATCAGCGCTGGTTCAGGTGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTg                                                          >  1:1082421/1‑93 (MQ=255)

                                 gCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtc                                                        >  1:2438829/1‑93 (MQ=255)

                                 gCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtc                                                        >  1:630556/1‑93 (MQ=255)

                                 gCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtc                                                        <  1:2696365/93‑1 (MQ=255)

                                  cACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcg                                                       >  1:5120308/1‑93 (MQ=255)

                                  cACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcg                                                       <  1:4310165/93‑1 (MQ=255)

                                    ccGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTgtcgtc                                                     >  1:1251056/1‑93 (MQ=255)

                                     cGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCa                                                    >  2:1512559/1‑93 (MQ=255)

                                     cGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCa                                                    >  1:3237161/1‑93 (MQ=255)

                                          gTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAc                                               >  2:3376774/1‑93 (MQ=255)

                                           tcatcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACg                                              >  2:1968633/1‑93 (MQ=255)

                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  1:169289/1‑93 (MQ=255)

                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  2:6123426/1‑93 (MQ=255)

                                             atcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAc                                            >  1:2198412/1‑93 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  1:2510534/93‑1 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           <  1:3082962/93‑1 (MQ=255)

                                              tcaGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACa                                           >  1:1061440/1‑93 (MQ=255)

                                               caGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAg                                          >  2:6195296/1‑93 (MQ=255)

                                               caGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAg                                          >  2:4260252/1‑93 (MQ=255)

                                               caGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAg                                          >  2:2107414/1‑93 (MQ=255)

                                                 gcgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        <  1:2542516/93‑1 (MQ=255)

                                                 gcgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        >  2:6295564/1‑93 (MQ=255)

                                                 gcgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        >  1:5414658/1‑93 (MQ=255)

                                                 gcgcTGATTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCa                                        <  1:4046377/93‑1 (MQ=255)

                                                  cgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAt                                       <  1:188829/93‑1 (MQ=255)

                                                  cgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAt                                       <  1:738699/93‑1 (MQ=255)

                                                    cTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaa                                     >  1:5782382/1‑93 (MQ=255)

                                                      ggTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaaa                                   <  2:3984771/93‑1 (MQ=255)

                                                       gTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATaaacg                                  <  1:4187055/93‑3 (MQ=255)

                                                        ttCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGc                                 >  2:2827357/1‑93 (MQ=255)

                                                        ttCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGc                                 <  2:710306/93‑1 (MQ=255)

                                                           aGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGAc                              <  1:4574587/93‑1 (MQ=255)

                                                           aGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGAc                              <  2:4314308/93‑1 (MQ=255)

                                                           aGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGAc                              <  1:5016566/93‑1 (MQ=255)

                                                            ggCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACa                             <  2:5155557/93‑1 (MQ=255)

                                                             gCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAg                            <  2:6026686/93‑1 (MQ=255)

                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  1:3939695/93‑1 (MQ=255)

                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  2:3612283/93‑1 (MQ=255)

                                                              cGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGc                           <  1:4821257/93‑1 (MQ=255)

                                                                tttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAt                         <  1:2298952/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  1:6219531/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        <  1:6620650/93‑1 (MQ=255)

                                                                 ttAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATc                        >  2:1619257/1‑93 (MQ=255)

                                                                  tAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATcc                       <  2:5506059/93‑1 (MQ=255)

                                                                   aGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCt                      <  1:4619513/93‑1 (MQ=255)

                                                                   aGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCt                      <  1:913306/93‑1 (MQ=255)

                                                                     tGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCg                    <  2:4045922/93‑1 (MQ=255)

                                                                     tGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCg                    >  1:3489540/1‑93 (MQ=255)

                                                                        gCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCa                 <  1:5230287/93‑1 (MQ=255)

                                                                        gCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCa                 <  2:3311730/93‑1 (MQ=255)

                                                                          aTTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGAGACAGCATCCTCGGCATg               >  1:2255619/1‑93 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  1:166055/1‑93 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  1:1277153/1‑93 (MQ=255)

                                                                           tttACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATgg              >  2:6474804/1‑93 (MQ=255)

                                                                             tACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt            <  2:1015850/93‑1 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  2:4661439/1‑93 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:2802450/1‑93 (MQ=255)

                                                                               cAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcg          >  1:5035994/1‑93 (MQ=255)

                                                                                aaGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgc         >  1:6553330/1‑93 (MQ=255)

                                                                                 aGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgcc        >  2:5121011/1‑93 (MQ=255)

                                                                                   cGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccgc      >  1:1748365/1‑93 (MQ=255)

                                                                                     gggTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCgg    >  1:15283/1‑93 (MQ=255)

                                                                                       gTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTa  >  2:3206364/1‑93 (MQ=255)

GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTA  >  NC_000913/2977834‑2978013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAA  >  NC_000913/2977831‑2978017

TGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTG                                                                                               >  SRR4302547.1728973/1‑94 (MQ=60)

TGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTG                                                                                               <  SRR4302547.5995189/94‑1 (MQ=60)

TGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTG                                                                                               >  SRR4302547.3527064/1‑94 (MQ=60)

TGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTG                                                                                               <  SRR4302547.5247715/94‑1 (MQ=60)

 GCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGCTCACGCGTTTAGTGGGCATTTACAAGCGGGGTTGC                                                                                              >  SRR4302547.44511/1‑94 (MQ=60)

  CGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGTTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCC                                                                                             <  SRR4302547.3070175/94‑1 (MQ=60)

  CGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCC                                                                                             <  SRR4302547.3135144/94‑1 (MQ=60)

  CGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCC                                                                                             >  SRR4302547.1850679/1‑94 (MQ=60)

   GGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGTGTTGCCG                                                                                            >  SRR4302547.4685987/1‑94 (MQ=60)

     TTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAA                                                                                          >  SRR4302547.3275573/1‑94 (MQ=60)

       CCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAC                                                                                        >  SRR4302547.1713853/1‑94 (MQ=60)

       CCGTTCACCGTCACCCTGATCCGCCCCCTGCGCCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAAC                                                                                        <  SRR4302547.6450215/94‑1 (MQ=60)

         GTTCACCGTCAGCCTGATCCGCCGCCTGCACCGCCCGTCATCAGCGCTGGCTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTGGCCGGAACTG                                                                                      >  SRR4302547.363696/1‑94 (MQ=60)

         GTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTG                                                                                      <  SRR4302547.862573/94‑1 (MQ=60)

          TGCACCGTCAGCCTGATCCGCCCCCTGCCCCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGG                                                                                     <  SRR4302547.2199895/94‑1 (MQ=60)

           TCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGT                                                                                    >  SRR4302547.6369881/1‑94 (MQ=60)

           TCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGT                                                                                    >  SRR4302547.95231/1‑94 (MQ=60)

              CCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCAC                                                                                 <  SRR4302547.4390836/94‑1 (MQ=60)

                GTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTA                                                                               <  SRR4302547.291121/94‑1 (MQ=60)

                 GCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTC                                                                              <  SRR4302547.5631943/94‑1 (MQ=60)

                  CAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCT                                                                             <  SRR4302547.3352812/94‑1 (MQ=60)

                   AGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTC                                                                            >  SRR4302547.6504579/1‑94 (MQ=60)

                    GCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCT                                                                           >  SRR4302547.5130367/1‑94 (MQ=60)

                     CCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTT                                                                          >  SRR4302547.1739686/1‑94 (MQ=60)

                     CCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTT                                                                          <  SRR4302547.4898347/94‑1 (MQ=60)

                     CCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTT                                                                          <  SRR4302547.6423755/94‑1 (MQ=60)

                      CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAATCTGGTCACTTCTCTTG                                                                         <  SRR4302547.397501/94‑1 (MQ=60)

                      CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCTCTTCTCTTG                                                                         <  SRR4302547.2125524/94‑1 (MQ=60)

                      CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         <  SRR4302547.5669140/94‑1 (MQ=60)

                      CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         >  SRR4302547.2977830/1‑94 (MQ=60)

                      CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         <  SRR4302547.1218325/94‑1 (MQ=60)

                      CTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTG                                                                         >  SRR4302547.851167/1‑94 (MQ=60)

                        GATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAC                                                                       <  SRR4302547.6494442/94‑1 (MQ=60)

                        GATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAC                                                                       >  SRR4302547.1174658/1‑94 (MQ=60)

                        GATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGAC                                                                       >  SRR4302547.3689566/1‑94 (MQ=60)

                          TCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGC                                                                     <  SRR4302547.2111249/94‑1 (MQ=60)

                             GCCCCCTGCACCTCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTAT                                                                  <  SRR4302547.118166/94‑1 (MQ=60)

                              CCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATT                                                                 >  SRR4302547.578891/1‑94 (MQ=60)

                              CCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATT                                                                 >  SRR4302547.6191449/1‑94 (MQ=60)

                               CCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTT                                                                >  SRR4302547.5611618/1‑94 (MQ=60)

                               CCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTT                                                                <  SRR4302547.4763316/94‑1 (MQ=60)

                               CCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTT                                                                >  SRR4302547.3246877/1‑94 (MQ=60)

                                CCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTT                                                               <  SRR4302547.57912/94‑1 (MQ=60)

                                CCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTT                                                               <  SRR4302547.949216/94‑1 (MQ=60)

                                CCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTT                                                               <  SRR4302547.3407884/94‑1 (MQ=60)

                                CCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTT                                                               >  SRR4302547.6553880/1‑94 (MQ=60)

                                 CCTGCACCGCCCGTCATCAGCGCTGGTTCAGGTGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTG                                                              >  SRR4302547.1104036/1‑94 (MQ=60)

                                 CCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTG                                                              <  SRR4302547.4778019/94‑1 (MQ=60)

                                   TGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTC                                                            >  SRR4302547.2488149/1‑94 (MQ=60)

                                   TGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTC                                                            >  SRR4302547.642206/1‑94 (MQ=60)

                                   TGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTC                                                            <  SRR4302547.648512/94‑1 (MQ=60)

                                    GCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCG                                                           >  SRR4302547.5225801/1‑94 (MQ=60)

                                    GCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCG                                                           <  SRR4302547.2750496/94‑1 (MQ=60)

                                    GCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCG                                                           >  SRR4302547.83446/1‑94 (MQ=60)

                                     CACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGT                                                          <  SRR4302547.4398829/94‑1 (MQ=60)

                                     CACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGT                                                          >  SRR4302547.5136510/1‑94 (MQ=60)

                                      ACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTC                                                         >  SRR4302547.1276305/1‑94 (MQ=60)

                                       CCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCA                                                        >  SRR4302547.85297/1‑94 (MQ=60)

                                       CCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCA                                                        >  SRR4302547.3301779/1‑94 (MQ=60)

                                           CCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTA                                                    >  SRR4302547.2669579/1‑94 (MQ=60)

                                            CGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAC                                                   <  SRR4302547.4082216/94‑1 (MQ=60)

                                             GTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACG                                                  >  SRR4302547.1420426/1‑94 (MQ=60)

                                             CTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACG                                                  <  SRR4302547.3625621/94‑1 (MQ=60)

                                               CATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAC                                                >  SRR4302547.2369483/1‑94 (MQ=60)

                                               CATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAC                                                >  SRR4302547.4013388/1‑94 (MQ=60)

                                               CATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAC                                                >  SRR4302547.2242574/1‑94 (MQ=60)

                                               CATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGAC                                                >  SRR4302547.172385/1‑94 (MQ=60)

                                                ATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACA                                               >  SRR4302547.2221927/1‑94 (MQ=60)

                                                ATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACA                                               >  SRR4302547.1082554/1‑94 (MQ=60)

                                                 TCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAG                                              <  SRR4302547.2561279/94‑1 (MQ=60)

                                                 TCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAG                                              <  SRR4302547.3144160/94‑1 (MQ=60)

                                                   AGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCA                                            >  SRR4302547.5526661/1‑94 (MQ=60)

                                                    GCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAT                                           <  SRR4302547.2593877/94‑1 (MQ=60)

                                                    GCGCTGATTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCAT                                           <  SRR4302547.4128541/94‑1 (MQ=60)

                                                     CGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATA                                          <  SRR4302547.752535/94‑1 (MQ=60)

                                                     CGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATA                                          >  SRR4302547.6787274/1‑94 (MQ=60)

                                                     CGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATA                                          <  SRR4302547.192385/94‑1 (MQ=60)

                                                      GCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAA                                         <  SRR4302547.5379420/94‑1 (MQ=60)

                                                      GCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAA                                         >  SRR4302547.2463954/1‑94 (MQ=60)

                                                      GCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAA                                         <  SRR4302547.2165947/94‑1 (MQ=60)

                                                      GCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAA                                         >  SRR4302547.5901979/1‑94 (MQ=60)

                                                        TGGTTCAGGCGTTTAGTGTGCATTTACAAGCGGGGTTCCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAA                                       <  SRR4302547.3346972/94‑1 (MQ=60)

                                                        TGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAA                                       >  SRR4302547.4248510/1‑94 (MQ=60)

                                                        TGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAA                                       >  SRR4302547.2647282/1‑94 (MQ=60)

                                                        TGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAA                                       >  SRR4302547.5866708/1‑94 (MQ=60)

                                                         GGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAG                                      >  SRR4302547.4597706/1‑94 (MQ=60)

                                                         GGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAG                                      >  SRR4302547.1698085/1‑94 (MQ=60)

                                                         GGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAG                                      <  SRR4302547.2540326/94‑1 (MQ=60)

                                                         GGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAG                                      <  SRR4302547.6745126/94‑1 (MQ=60)

                                                         GGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAG                                      <  SRR4302547.5195164/94‑1 (MQ=60)

                                                          GTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGGCAGCATAAAAGC                                     >  SRR4302547.1883631/1‑94 (MQ=60)

                                                          GTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAACGC                                     <  SRR4302547.4272431/94‑1 (MQ=60)

                                                          GTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGC                                     >  SRR4302547.4761668/1‑94 (MQ=60)

                                                          GTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGC                                     >  SRR4302547.5756861/1‑94 (MQ=60)

                                                           TTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCG                                    <  SRR4302547.858222/94‑1 (MQ=60)

                                                            TCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGA                                   >  SRR4302547.6207698/1‑94 (MQ=60)

                                                             CAGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGAC                                  <  SRR4302547.2912946/94‑1 (MQ=60)

                                                              AGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACA                                 <  SRR4302547.4669250/94‑1 (MQ=60)

                                                              AGGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACA                                 <  SRR4302547.5120254/94‑1 (MQ=60)

                                                              AGGCGTTTAGTGGACATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACA                                 >  SRR4302547.1217065/1‑94 (MQ=60)

                                                               GGCGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAG                                >  SRR4302547.1943997/1‑94 (MQ=60)

                                                                 CGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCA                              <  SRR4302547.4921172/94‑1 (MQ=60)

                                                                 CGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCA                              >  SRR4302547.910566/1‑94 (MQ=60)

                                                                 CGTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCA                              <  SRR4302547.4019800/94‑1 (MQ=60)

                                                                  GTTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCAT                             <  SRR4302547.6737048/94‑1 (MQ=60)

                                                                   TTTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATC                            <  SRR4302547.2345541/94‑1 (MQ=60)

                                                                    TTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACTGCATAAAAGCGACAGCATCC                           <  SRR4302547.6629886/94‑1 (MQ=60)

                                                                    TTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCC                           <  SRR4302547.6758456/94‑1 (MQ=60)

                                                                    TTAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCC                           <  SRR4302547.6348688/94‑1 (MQ=60)

                                                                     TAGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCT                          <  SRR4302547.1285391/94‑1 (MQ=60)

                                                                      AGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTC                         <  SRR4302547.4715167/94‑1 (MQ=60)

                                                                      AGTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTC                         <  SRR4302547.930917/94‑1 (MQ=60)

                                                                       GTGGGCATTTACAAGCGGGGTTGCGGAATCTGGTCTCTGCTCTTGACTCTATTTTGTCGTCAGCGACGACAGCATAAAGGCGACAGCATCCTCG                        <  SRR4302547.6449390/94‑1 (MQ=60)

                                                                       GTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCG                        <  SRR4302547.2565151/94‑1 (MQ=60)

                                                                       GTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCG                        >  SRR4302547.3560744/1‑94 (MQ=60)

                                                                       GTGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCG                        >  SRR4302547.2281954/1‑94 (MQ=60)

                                                                        TGGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGG                       <  SRR4302547.5268742/94‑1 (MQ=60)

                                                                         GGGCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGC                      <  SRR4302547.6354996/94‑1 (MQ=60)

                                                                           GCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCAT                    <  SRR4302547.4199081/94‑1 (MQ=60)

                                                                           GCATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCAT                    <  SRR4302547.5337948/94‑1 (MQ=60)

                                                                            CATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATG                   <  SRR4302547.5996424/94‑1 (MQ=60)

                                                                            CATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGAGACAGCATCCTCGGCATG                   >  SRR4302547.2301172/1‑94 (MQ=60)

                                                                             ATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGG                  >  SRR4302547.1302875/1‑94 (MQ=60)

                                                                             ATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGG                  >  SRR4302547.169105/1‑94 (MQ=60)

                                                                             ATTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTAAGACAGCATAAAAGCGACAGCATCCTCGGCATGG                  >  SRR4302547.1585523/1‑94 (MQ=60)

                                                                             AATTACAAGCGGGGTTGCCGAAGCTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGG                  <  SRR4302547.1095070/94‑1 (MQ=60)

                                                                              TTTACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGA                 >  SRR4302547.6220050/1‑94 (MQ=60)

                                                                                TACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATC               <  SRR4302547.5035969/94‑1 (MQ=60)

                                                                                 ACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCG              <  SRR4302547.3523065/94‑1 (MQ=60)

                                                                                 ACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCG              >  SRR4302547.2858555/1‑94 (MQ=60)

                                                                                 ACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCG              >  SRR4302547.5140015/1‑94 (MQ=60)

                                                                                 ACAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCG              <  SRR4302547.1359052/94‑1 (MQ=60)

                                                                                  CAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGC             >  SRR4302547.6689751/1‑94 (MQ=60)

                                                                                  CAAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGC             >  SRR4302547.2819477/1‑94 (MQ=60)

                                                                                   AAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCC            <  SRR4302547.640675/94‑1 (MQ=60)

                                                                                   AAGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCC            <  SRR4302547.2333066/94‑1 (MQ=60)

                                                                                    AGCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCG           <  SRR4302547.4566708/94‑1 (MQ=60)

                                                                                     GCGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGC          >  SRR4302547.1782869/1‑94 (MQ=60)

                                                                                      CGGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCG         <  SRR4302547.2296528/94‑1 (MQ=60)

                                                                                       GGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGG        >  SRR4302547.15564/1‑94 (MQ=60)

                                                                                       GGGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGG        >  SRR4302547.2861519/1‑94 (MQ=60)

                                                                                        GGGTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGT       >  SRR4302547.5267719/1‑94 (MQ=60)

                                                                                          GTTGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTAT     >  SRR4302547.6764221/1‑94 (MQ=60)

                                                                                            TGCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCA   <  SRR4302547.2933339/94‑1 (MQ=60)

                                                                                             GCCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAA  <  SRR4302547.5944243/94‑1 (MQ=60)

TGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAA  >  NC_000913/2977831‑2978017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`