Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,059,022 T→C G51G (GGT→GGC)  sbm → methylmalonyl‑CoA mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,059,0220TC100.0% 786.5 / NA 219G51G (GGT→GGC) sbmmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (115/104);  total (115/104)

Only 100 of 221 total aligned reads displayed.

TGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTT  >  NC_000913/3058932‑3059111
                                                                                          |                                                                                         
tGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGTAGGTGACAGGCAc                                                                                         >  2:4523197/1‑93 (MQ=255)
tGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCAc                                                                                         <  2:4878735/93‑1 (MQ=255)
  tCGCCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCAccc                                                                                       <  1:4594079/93‑1 (MQ=255)
  tCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCAccc                                                                                       <  1:3697367/93‑1 (MQ=255)
  tCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCAccc                                                                                       <  2:15731/93‑1 (MQ=255)
   cGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCt                                                                                      >  1:899113/1‑93 (MQ=255)
    gACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCtt                                                                                     >  2:2584909/1‑93 (MQ=255)
     aCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTc                                                                                    >  2:1299408/1‑93 (MQ=255)
     aCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTc                                                                                    <  2:2847181/93‑1 (MQ=255)
     aCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTc                                                                                    >  1:2599748/1‑93 (MQ=255)
      cTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTcc                                                                                   <  2:5717443/93‑1 (MQ=255)
       tCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCt                                                                                  <  2:4006829/93‑1 (MQ=255)
       tCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCt                                                                                  >  1:1731529/1‑93 (MQ=255)
       tCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCt                                                                                  <  1:1408915/93‑1 (MQ=255)
       tCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCt                                                                                  >  1:1303130/1‑93 (MQ=255)
       tCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCt                                                                                  >  2:6154565/1‑93 (MQ=255)
        cGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTg                                                                                 <  2:5518929/93‑1 (MQ=255)
         gCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTgg                                                                                >  1:1153284/1‑93 (MQ=255)
          cTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGt                                                                               >  2:2298471/1‑93 (MQ=255)
            ggTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTACCTGGttt                                                                             <  1:6502682/93‑1 (MQ=255)
                catcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccg                                                                         >  1:3329475/1‑93 (MQ=255)
                 atcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccgc                                                                        >  2:1671883/1‑93 (MQ=255)
                  tcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccgcc                                                                       >  2:5838746/1‑93 (MQ=255)
                  tcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccgcc                                                                       >  2:5243110/1‑93 (MQ=255)
                  tcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccgcc                                                                       <  2:2709643/93‑1 (MQ=255)
                   caAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGccc                                                                      <  1:2286111/93‑1 (MQ=255)
                    aaaCCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCt                                                                     <  1:5292319/93‑1 (MQ=255)
                    aaaCCGCGGAAGGGATCGCCAACAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCt                                                                     <  1:5943805/93‑1 (MQ=255)
                       ccGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACg                                                                  <  1:4381098/93‑1 (MQ=255)
                         gcgGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGtt                                                                <  2:3798729/93‑1 (MQ=255)
                          cgGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTc                                                               <  2:631273/93‑1 (MQ=255)
                           ggAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCg                                                              <  1:5513172/93‑1 (MQ=255)
                           ggAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCg                                                              >  1:4409103/1‑93 (MQ=255)
                             aaGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTg                                                            <  2:8543/93‑1 (MQ=255)
                              aGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTgg                                                           >  1:2984210/1‑93 (MQ=255)
                              aGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTgg                                                           <  2:2231420/93‑1 (MQ=255)
                               gggATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGc                                                          >  1:6131314/1‑93 (MQ=255)
                                ggATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGcc                                                         <  1:6079956/93‑1 (MQ=255)
                                  aTCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcg                                                       <  2:4231249/93‑1 (MQ=255)
                                  aTCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcg                                                       <  1:1854540/93‑1 (MQ=255)
                                   tCGCCGTCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgc                                                      >  2:2429145/1‑93 (MQ=255)
                                   tCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACTTTCGTGGCCcgc                                                      <  2:3585918/93‑1 (MQ=255)
                                     gCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGt                                                    >  1:2866608/1‑93 (MQ=255)
                                     gCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGt                                                    >  2:1544947/1‑93 (MQ=255)
                                      ccATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                   >  2:3110360/1‑93 (MQ=255)
                                       cATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGc                                                  >  2:1213192/1‑93 (MQ=255)
                                         tCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCa                                                <  2:2060731/93‑1 (MQ=255)
                                         tCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCa                                                >  2:3935183/1‑93 (MQ=255)
                                          cAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCAc                                               <  1:1061697/93‑1 (MQ=255)
                                          cAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCAc                                               <  1:5582215/93‑1 (MQ=255)
                                          cAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCAc                                               >  1:6382494/1‑93 (MQ=255)
                                             gCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTAt                                            >  1:5789083/1‑93 (MQ=255)
                                               cGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGt                                          >  1:4785188/1‑93 (MQ=255)
                                               cGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGt                                          >  2:3476578/1‑93 (MQ=255)
                                                gCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGta                                         <  2:5661583/93‑1 (MQ=255)
                                                 cTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGtat                                        <  2:1263756/93‑1 (MQ=255)
                                                 cTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGtat                                        >  2:2360746/1‑93 (MQ=255)
                                                  tGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGtata                                       <  1:5137434/93‑1 (MQ=255)
                                                   gTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAc                                      <  1:3903260/93‑1 (MQ=255)
                                                   gTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAc                                      >  1:5453088/1‑93 (MQ=255)
                                                    tataCCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAcc                                     >  2:3240551/1‑93 (MQ=255)
                                                      taCCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGc                                   <  1:4142181/93‑1 (MQ=255)
                                                       aCCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGcc                                  <  2:623900/93‑1 (MQ=255)
                                                        ccGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGccc                                 <  1:1791739/93‑1 (MQ=255)
                                                          gAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCaa                               <  1:2611527/93‑1 (MQ=255)
                                                           aaGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAc                              >  2:5800545/1‑93 (MQ=255)
                                                           aaGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAc                              <  1:3777988/93‑1 (MQ=255)
                                                            aGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAcc                             >  1:2496541/1‑93 (MQ=255)
                                                              ccGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGt                           <  1:6250872/93‑1 (MQ=255)
                                                               cGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTg                          <  2:436263/93‑1 (MQ=255)
                                                                gATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTgg                         <  2:4603023/93‑1 (MQ=255)
                                                                 aTCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGa                        >  1:168914/1‑93 (MQ=255)
                                                                 aTCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGa                        <  2:5360155/93‑1 (MQ=255)
                                                                 aTCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGa                        <  2:1779203/93‑1 (MQ=255)
                                                                 aTCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGa                        >  2:4730453/1‑93 (MQ=255)
                                                                 aTCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGa                        >  2:427833/1‑93 (MQ=255)
                                                                  tctcGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAc                       >  1:360540/1‑93 (MQ=255)
                                                                    tcGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGTCCCCGTGCCACTATGTATACCGCCCAACCGTGGAcct                     >  1:592862/1‑92 (MQ=255)
                                                                    tcGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCa                     >  1:1194369/1‑93 (MQ=255)
                                                                    tcGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCa                     >  1:2673245/1‑93 (MQ=255)
                                                                    tcGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAACGCCCAACCGTGGACCa                     >  2:5964380/1‑93 (MQ=255)
                                                                      gATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATc                   >  1:430998/1‑93 (MQ=255)
                                                                      gATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATc                   <  1:5716127/93‑1 (MQ=255)
                                                                      gATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATc                   <  2:6051463/93‑1 (MQ=255)
                                                                      gATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATc                   >  2:5342029/1‑93 (MQ=255)
                                                                       aTAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATcc                  >  2:3331671/1‑93 (MQ=255)
                                                                       aTAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATcc                  <  1:4546727/93‑1 (MQ=255)
                                                                        tAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCg                 <  2:4452145/93‑1 (MQ=255)
                                                                          aTCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTc               <  2:209023/93‑1 (MQ=255)
                                                                           tCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCa              >  2:746175/1‑93 (MQ=255)
                                                                           tCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCa              >  1:701744/1‑93 (MQ=255)
                                                                              ggAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTa           >  2:4833359/1‑93 (MQ=255)
                                                                               gAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTAt          <  1:659309/93‑1 (MQ=255)
                                                                                 ggTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGc        >  2:1378389/1‑93 (MQ=255)
                                                                                 ggTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGc        <  2:1492246/93‑1 (MQ=255)
                                                                                   tGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTg      <  2:3063258/93‑1 (MQ=255)
                                                                                    gACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTgg     <  1:3089028/93‑1 (MQ=255)
                                                                                      cAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGtt   >  2:6601342/1‑93 (MQ=255)
                                                                                      cAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGtt   >  2:101955/1‑93 (MQ=255)
                                                                                      cAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGtt   <  2:925239/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTT  >  NC_000913/3058932‑3059111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3058929‑3059115
                                                                                             |                                                                                             
AACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGC                                                                                               <  SRR4302547.6002256/94‑1 (MQ=60)
  CTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCAC                                                                                             <  SRR4302547.1059838/94‑1 (MQ=60)
  CTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCAC                                                                                             >  SRR4302547.668203/1‑94 (MQ=60)
   TGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACC                                                                                            >  SRR4302547.3345902/1‑94 (MQ=60)
     TCGCCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCT                                                                                          <  SRR4302547.4689141/94‑1 (MQ=60)
     TCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCT                                                                                          <  SRR4302547.3772829/94‑1 (MQ=60)
     TCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCT                                                                                          >  SRR4302547.916399/1‑94 (MQ=60)
      CGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTT                                                                                         <  SRR4302547.1090077/94‑1 (MQ=60)
      CGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTT                                                                                         >  SRR4302547.4441844/1‑94 (MQ=60)
      CGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTT                                                                                         >  SRR4302547.2661688/1‑94 (MQ=60)
       GACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTC                                                                                        >  SRR4302547.2652154/1‑94 (MQ=60)
       GACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTC                                                                                        <  SRR4302547.4285433/94‑1 (MQ=60)
        ACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCC                                                                                       <  SRR4302547.495715/94‑1 (MQ=60)
        ACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCC                                                                                       >  SRR4302547.446003/1‑94 (MQ=60)
         CTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      >  SRR4302547.1765544/1‑94 (MQ=60)
         CTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      >  SRR4302547.1329348/1‑94 (MQ=60)
         CTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      >  SRR4302547.3731442/1‑94 (MQ=60)
         CTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      >  SRR4302547.5889941/1‑94 (MQ=60)
          TCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTG                                                                                     <  SRR4302547.1437098/94‑1 (MQ=60)
           CGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGG                                                                                    >  SRR4302547.1176626/1‑94 (MQ=60)
               GGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTG                                                                                >  SRR4302547.1732341/1‑94 (MQ=60)
               GGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTACCTGGTTTG                                                                                <  SRR4302547.6638036/94‑1 (MQ=60)
                GTTCATCAAACCGCGGGAGGGATCGCCATCAAGCCGCTGTGTACCGAAGCCGATCTCGATAATCTGGAGGCGGCAGGCACCCTTCCTGGTTTGC                                                                               >  SRR4302547.471609/1‑94 (MQ=60)
                  TCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCG                                                                             >  SRR4302547.2471223/1‑94 (MQ=60)
                  TCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCG                                                                             >  SRR4302547.3396799/1‑94 (MQ=60)
                      CAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCT                                                                         <  SRR4302547.2332395/94‑1 (MQ=60)
                      CAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCT                                                                         >  SRR4302547.6212191/1‑94 (MQ=60)
                       AAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTA                                                                        >  SRR4302547.1428119/1‑94 (MQ=60)
                       AAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTA                                                                        <  SRR4302547.5401501/94‑1 (MQ=60)
                       AAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTA                                                                        >  SRR4302547.6651018/1‑94 (MQ=60)
                       AAACCGCGGAAGGGATCGCCAACAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTA                                                                        <  SRR4302547.6067059/94‑1 (MQ=60)
                         ACGGCGGAAGGGATGGCAATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACG                                                                      <  SRR4302547.2925250/94‑1 (MQ=60)
                          CCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGT                                                                     <  SRR4302547.4471183/94‑1 (MQ=60)
                           CGCGGAGGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTT                                                                    <  SRR4302547.2675752/94‑1 (MQ=60)
                             CGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCG                                                                  >  SRR4302547.4499976/1‑94 (MQ=60)
                              GGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGT                                                                 <  SRR4302547.5626984/94‑1 (MQ=60)
                               GAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTG                                                                >  SRR4302547.6437354/1‑94 (MQ=60)
                                AAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGG                                                               >  SRR4302547.3043599/1‑94 (MQ=60)
                                 AGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGC                                                              >  SRR4302547.6064160/1‑94 (MQ=60)
                                 AGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGC                                                              >  SRR4302547.6258495/1‑94 (MQ=60)
                                  ggaaccgcccTCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCC                                                             <  SRR4302547.43991/84‑1 (MQ=60)
                                   GGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCC                                                            <  SRR4302547.6206069/94‑1 (MQ=60)
                                     ATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGC                                                          <  SRR4302547.1537412/94‑1 (MQ=60)
                                     ATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGC                                                          <  SRR4302547.2402987/94‑1 (MQ=60)
                                     ATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGC                                                          <  SRR4302547.1891184/94‑1 (MQ=60)
                                       CGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGT                                                        >  SRR4302547.2923870/1‑94 (MQ=60)
                                          CATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCC                                                     >  SRR4302547.5569052/1‑94 (MQ=60)
                                          CATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCC                                                     >  SRR4302547.3804910/1‑94 (MQ=60)
                                            TCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCAC                                                   >  SRR4302547.6515689/1‑94 (MQ=60)
                                             CAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACT                                                  <  SRR4302547.5697354/94‑1 (MQ=60)
                                             CAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACT                                                  <  SRR4302547.1082818/94‑1 (MQ=60)
                                              AAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTTCGTTCGTGGTCCGCGTGCCAgtt                                                 >  SRR4302547.5066056/1‑91 (MQ=60)
                                               AGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTAT                                                >  SRR4302547.5908833/1‑94 (MQ=60)
                                                 CCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGT                                              <  SRR4302547.4213097/94‑1 (MQ=60)
                                                 CCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGT                                              >  SRR4302547.4884304/1‑94 (MQ=60)
                                                  CGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTA                                             >  SRR4302547.4674616/1‑94 (MQ=60)
                                                   GCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTAT                                            <  SRR4302547.58038/94‑1 (MQ=60)
                                                    CTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATA                                           >  SRR4302547.3606137/1‑94 (MQ=60)
                                                     TGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAC                                          >  SRR4302547.5565772/1‑94 (MQ=60)
                                                     TGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAC                                          <  SRR4302547.5243271/94‑1 (MQ=60)
                                                      GTATGCCGAAGCCGATCTCGATAATCTGGAGGTGACATGCACCCTTCCTGGTTTGCCACACTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         <  SRR4302547.4285949/94‑1 (MQ=60)
                                                      GTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         >  SRR4302547.4472000/1‑94 (MQ=60)
                                                      GTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         >  SRR4302547.6324617/1‑94 (MQ=60)
                                                      GTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         <  SRR4302547.3982634/94‑1 (MQ=60)
                                                       TATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCG                                        >  SRR4302547.3829579/1‑94 (MQ=60)
                                                         TACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCC                                      <  SRR4302547.6536666/94‑1 (MQ=60)
                                                         TACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCC                                      <  SRR4302547.4226477/94‑1 (MQ=60)
                                                          ACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCC                                     <  SRR4302547.1192336/94‑1 (MQ=60)
                                                           CCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCA                                    <  SRR4302547.1827218/94‑1 (MQ=60)
                                                            CGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAA                                   <  SRR4302547.2116509/94‑1 (MQ=60)
                                                             GAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTTGTTTGCCGCCCTACGTTCGTGGCCAGCGTGCCACTATGTATAGCGACCAAC                                  >  SRR4302547.5771936/1‑94 (MQ=60)
                                                             GAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAC                                  <  SRR4302547.2664149/94‑1 (MQ=60)
                                                              AAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACC                                 <  SRR4302547.3855032/94‑1 (MQ=60)
                                                              AAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACC                                 >  SRR4302547.2546981/1‑94 (MQ=60)
                                                              AAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACC                                 >  SRR4302547.501165/1‑94 (MQ=60)
                                                                 CCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTG                              <  SRR4302547.6380725/94‑1 (MQ=60)
                                                                  CGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGG                             >  SRR4302547.1800574/1‑94 (MQ=60)
                                                                   GATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGA                            >  SRR4302547.2996098/1‑94 (MQ=60)
                                                                   GATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGA                            >  SRR4302547.2573031/1‑94 (MQ=60)
                                                                   GATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGA                            >  SRR4302547.172007/1‑94 (MQ=60)
                                                                   GATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGA                            >  SRR4302547.1324681/1‑94 (MQ=60)
                                                                    ATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAC                           >  SRR4302547.367227/1‑94 (MQ=60)
                                                                     TCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACC                          <  SRR4302547.5693569/94‑1 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGTCCCCGTGCCACTATGTATACCGCCCAACCGTGGACCT                         >  SRR4302547.603685/1‑94 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCA                         <  SRR4302547.1837104/94‑1 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCA                         >  SRR4302547.1218473/1‑94 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCA                         >  SRR4302547.2726943/1‑94 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCA                         <  SRR4302547.2878055/94‑1 (MQ=60)
                                                                        CGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATC                       >  SRR4302547.438978/1‑94 (MQ=60)
                                                                        CGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAACGTGGACCATC                       >  SRR4302547.5995441/1‑94 (MQ=60)
                                                                        CGATAATCTGGAAGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATC                       <  SRR4302547.5496514/94‑1 (MQ=60)
                                                                         GATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCC                      <  SRR4302547.6197887/94‑1 (MQ=60)
                                                                         GATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCC                      <  SRR4302547.5834178/94‑1 (MQ=60)
                                                                          ATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCG                     <  SRR4302547.4640747/94‑1 (MQ=60)
                                                                           TAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGT                    <  SRR4302547.623573/94‑1 (MQ=60)
                                                                            AATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTC                   <  SRR4302547.3981229/94‑1 (MQ=60)
                                                                             ATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCA                  >  SRR4302547.714713/1‑94 (MQ=60)
                                                                              TCTGGAGCTGACAGCCACCCTTCCTGGTTTGCTGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAG                 <  SRR4302547.1859168/94‑1 (MQ=60)
                                                                                 GGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTAT              <  SRR4302547.3076754/94‑1 (MQ=60)
                                                                                  GAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATG             <  SRR4302547.671453/94‑1 (MQ=60)
                                                                                    GGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCT           >  SRR4302547.4812123/1‑94 (MQ=60)
                                                                                    GGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCT           <  SRR4302547.4507249/94‑1 (MQ=60)
                                                                                    GGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCT           >  SRR4302547.6777712/1‑94 (MQ=60)
                                                                                      TGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGG         <  SRR4302547.5340023/94‑1 (MQ=60)
                                                                                      TGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGG         <  SRR4302547.1639352/94‑1 (MQ=60)
                                                                                       GACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGT        <  SRR4302547.3150300/94‑1 (MQ=60)
                                                                                         CAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTT      <  SRR4302547.914045/94‑1 (MQ=60)
                                                                                          AGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTT     >  SRR4302547.2899262/1‑94 (MQ=60)
                                                                                            GCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTC   >  SRR4302547.5334802/1‑94 (MQ=60)
                                                                                            GCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTC   >  SRR4302547.5483162/1‑94 (MQ=60)
                                                                                            GCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTC   <  SRR4302547.1119790/94‑1 (MQ=60)
                                                                                             CACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  SRR4302547.543752/1‑94 (MQ=60)
                                                                                             CACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  <  SRR4302547.4285390/94‑1 (MQ=60)
                                                                                             |                                                                                             
AACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3058929‑3059115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑