Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,072,672	T→C	Q13R (CAG→CGG)	yggC ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,072,672	0	T	C	84.6%	612.1 / 100.8	240	Q13R (CAG→CGG)	yggC	putative kinase
Reads supporting (aligned to +/- strand): ref base T (19/18); new base C (114/89); total (133/107)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

Only 100 of 243 total aligned reads displayed.

GCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTG  >  NC_000913/3072583‑3072760

 ccgcTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTAt                                                                                      >  2:3101188/1‑93 (MQ=255)

   gcTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCt                                                                                    <  2:3817861/93‑1 (MQ=255)

   gcTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCt                                                                                    >  1:2060599/1‑93 (MQ=255)

     tGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTc                                                                                  <  1:844848/93‑1 (MQ=255)

     tGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTc                                                                                  <  2:4174016/93‑1 (MQ=255)

      gCGGATTTACCGTCTGTAAGGACGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCa                                                                                 <  1:5968241/93‑1 (MQ=255)

       cGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAg                                                                                <  2:4016441/93‑1 (MQ=255)

        ggATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGc                                                                               <  1:800734/93‑1 (MQ=255)

        ggATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGc                                                                               <  1:5731963/93‑1 (MQ=255)

         gATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGcc                                                                              >  2:490480/1‑93 (MQ=255)

         gATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGcc                                                                              >  1:4337950/1‑93 (MQ=255)

           tttACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCa                                                                            <  1:3035871/93‑1 (MQ=255)

           tttACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCa                                                                            >  2:5349735/1‑93 (MQ=255)

           tttACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCa                                                                            >  1:4753370/1‑93 (MQ=255)

           tttACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCa                                                                            <  1:5800309/93‑1 (MQ=255)

             tatcGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCAtt                                                                          <  2:2634556/90‑1 (MQ=255)

              aCCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTc                                                                         <  2:2687387/93‑1 (MQ=255)

              aCCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTc                                                                         >  1:1621648/1‑93 (MQ=255)

                cGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCAc                                                                       >  2:5306727/1‑93 (MQ=255)

                 gTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCAcc                                                                      <  1:2565166/93‑1 (MQ=255)

                  tCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCg                                                                     >  1:6609075/1‑93 (MQ=255)

                   cTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGt                                                                    <  1:3844962/93‑1 (MQ=255)

                    tGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGtt                                                                   <  1:3587992/93‑1 (MQ=255)

                    tGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGtt                                                                   >  2:1581832/1‑93 (MQ=255)

                        aGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAAtt                                                               <  1:703073/93‑1 (MQ=255)

                        aGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAAtt                                                               >  2:1331863/1‑93 (MQ=255)

                         ggCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTc                                                              <  2:1440476/93‑1 (MQ=255)

                         ggCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGACCGTATCTTCAGCCCATTCACCGTTAATTc                                                              >  2:5439743/1‑93 (MQ=255)

                           ccgccAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCaa                                                            <  1:1360854/93‑1 (MQ=255)

                            cgccAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAAt                                                           >  2:1184361/1‑93 (MQ=255)

                             gccAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAAtt                                                          >  1:1248830/1‑93 (MQ=255)

                              ccAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCACttt                                                         >  2:387724/1‑93 (MQ=255)

                               cAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAAtttt                                                        >  2:4223656/1‑93 (MQ=255)

                               cAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAAtttt                                                        >  2:1122207/1‑93 (MQ=255)

                                aaCATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTc                                                       <  1:1016706/93‑1 (MQ=255)

                                aaCATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTc                                                       >  1:1856684/1‑93 (MQ=255)

                                 aCATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCa                                                      <  1:39707/93‑1 (MQ=255)

                                 aCATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCa                                                      >  2:4484825/1‑93 (MQ=255)

                                     gtgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTATTGTGCCCGTTTCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGc                                                  >  2:6646449/1‑93 (MQ=255)

                                     gtgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGc                                                  <  2:1712273/93‑1 (MQ=255)

                                     gtgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGc                                                  <  1:4710624/93‑1 (MQ=255)

                                      tgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCg                                                 <  2:2351989/93‑1 (MQ=255)

                                      tgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCg                                                 <  1:2964343/93‑1 (MQ=255)

                                        cagcaACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGtt                                               <  1:1771668/93‑1 (MQ=255)

                                         agcaACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTa                                              <  2:1357130/93‑1 (MQ=255)

                                          gcaTCGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                                             <  1:3654331/93‑1 (MQ=255)

                                          gcaACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                                             >  1:3196514/1‑93 (MQ=255)

                                           caACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAAt                                            <  2:6271994/93‑1 (MQ=255)

                                            aaCGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCCTTCACCGTTAATTCAATTTTCACGGCGTTaact                                           >  1:4408597/1‑91 (MQ=255)

                                            aaCGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAAtt                                           <  2:5629320/93‑1 (MQ=255)

                                             aCGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTc                                          <  1:242451/93‑1 (MQ=255)

                                             aCGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTc                                          >  2:775145/1‑93 (MQ=255)

                                              cGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTcc                                         >  1:1069095/1‑93 (MQ=255)

                                               ggTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCt                                        <  1:944097/93‑1 (MQ=255)

                                               ggTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCt                                        <  1:5524142/93‑1 (MQ=255)

                                               ggTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTTATTCCt                                        >  2:4561345/1‑93 (MQ=255)

                                               ggTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCt                                        >  1:1212162/1‑93 (MQ=255)

                                                gTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCtt                                       >  2:4393971/1‑93 (MQ=255)

                                                   tATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTaa                                    >  1:5651129/1‑93 (MQ=255)

                                                   tATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTaa                                    >  2:185890/1‑93 (MQ=255)

                                                   tATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTaa                                    >  2:3694112/1‑93 (MQ=255)

                                                   tATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTaa                                    >  2:2136433/1‑93 (MQ=255)

                                                   tATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTaa                                    >  2:6517538/1‑93 (MQ=255)

                                                   tATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTaa                                    >  2:4580890/1‑93 (MQ=255)

                                                    aTGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAg                                   >  1:2664980/1‑93 (MQ=255)

                                                     tGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAgg                                  <  2:3627384/93‑1 (MQ=255)

                                                     tGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAgg                                  <  2:2825223/93‑1 (MQ=255)

                                                      gTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAggt                                 <  1:3483168/93‑1 (MQ=255)

                                                      gTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAggt                                 >  2:5852113/1‑93 (MQ=255)

                                                        aCATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAggtgg                               >  2:5155738/1‑93 (MQ=255)

                                                           ttttCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCg                            >  2:2015673/1‑93 (MQ=255)

                                                              tCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTa                         >  1:4261657/1‑93 (MQ=255)

                                                               cAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTAc                        >  2:4749002/1‑93 (MQ=255)

                                                                aaTCTCTTCGTTCTGGTACCGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACt                       >  2:5954328/1‑93 (MQ=255)

                                                                 aTCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTg                      >  1:1077015/1‑93 (MQ=255)

                                                                   ctctTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGcc                    <  2:5987802/93‑1 (MQ=255)

                                                                   ctctTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGcc                    >  1:1397212/1‑93 (MQ=255)

                                                                    tctTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCa                   >  2:6588867/1‑93 (MQ=255)

                                                                    tctTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCa                   <  1:2024037/93‑1 (MQ=255)

                                                                      ttCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATGCCTTTAAGGTGGTCGTTACTGCCAGc                 >  1:5360935/1‑93 (MQ=255)

                                                                        cGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGt               >  1:3530668/1‑93 (MQ=255)

                                                                        cGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGt               <  1:1907634/93‑1 (MQ=255)

                                                                        cGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGt               <  2:550614/93‑1 (MQ=255)

                                                                         gTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTc              >  2:2576653/1‑93 (MQ=255)

                                                                          ttCTGGAACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGGCAGCGTCa             >  1:5204787/1‑93 (MQ=255)

                                                                           tCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAg            >  1:6166804/1‑93 (MQ=255)

                                                                           tCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAg            <  2:2503876/93‑1 (MQ=255)

                                                                            cTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAgc           <  1:4968054/93‑1 (MQ=255)

                                                                             tGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAgcg          >  1:5062468/1‑93 (MQ=255)

                                                                              ggTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAgcgc         <  1:2280894/93‑1 (MQ=255)

                                                                              ggTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAgcgc         <  1:1734790/93‑1 (MQ=255)

                                                                              ggTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAgcgc         <  1:4550446/93‑1 (MQ=255)

                                                                               gTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGcc        >  2:5925540/1‑93 (MQ=255)

                                                                                tACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCg       >  2:942148/1‑93 (MQ=255)

                                                                                 aCTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGt      <  1:5424033/93‑1 (MQ=255)

                                                                                  cTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGtt     >  1:5673868/1‑93 (MQ=255)

                                                                                   tgtgCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGttt    <  2:336301/93‑1 (MQ=255)

                                                                                    gtgCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGtttt   >  1:4801175/1‑93 (MQ=255)

                                                                                    gtgCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGtttt   <  2:3544968/93‑1 (MQ=255)

                                                                                     tgCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTg  >  1:6279527/1‑93 (MQ=255)

GCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTG  >  NC_000913/3072583‑3072760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTACGCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCAT  >  NC_000913/3072579‑3072765

GTACGCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCC                                                                                               <  SRR4302547.4876828/94‑1 (MQ=60)

   CGCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTA                                                                                            >  SRR4302547.3343606/1‑94 (MQ=60)

    GCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTAT                                                                                           <  SRR4302547.1581730/94‑1 (MQ=60)

      CGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCT                                                                                         <  SRR4302547.2383730/94‑1 (MQ=60)

      CGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCT                                                                                         >  SRR4302547.2101716/1‑94 (MQ=60)

       GCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTT                                                                                        >  SRR4302547.198176/1‑94 (MQ=60)

        CTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTC                                                                                       <  SRR4302547.4031995/94‑1 (MQ=60)

         TGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCA                                                                                      <  SRR4302547.860973/94‑1 (MQ=60)

          GCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAG                                                                                     >  SRR4302547.1781468/1‑94 (MQ=60)

          GCGGATTTACCGTCTGTAAGGACGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAG                                                                                     <  SRR4302547.6092030/94‑1 (MQ=60)

           CGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGC                                                                                    >  SRR4302547.6695142/1‑94 (MQ=60)

            GGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCC                                                                                   <  SRR4302547.815898/94‑1 (MQ=60)

            GGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCC                                                                                   <  SRR4302547.5850391/94‑1 (MQ=60)

            GGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCC                                                                                   >  SRR4302547.4427178/1‑94 (MQ=60)

              ATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCA                                                                                 >  SRR4302547.1333215/1‑94 (MQ=60)

              ATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCA                                                                                 >  SRR4302547.4851875/1‑94 (MQ=60)

              ATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCA                                                                                 <  SRR4302547.5225765/94‑1 (MQ=60)

               TTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCAT                                                                                >  SRR4302547.1389810/1‑94 (MQ=60)

               TTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCAT                                                                                <  SRR4302547.3096266/94‑1 (MQ=60)

               TTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCAT                                                                                >  SRR4302547.567628/1‑94 (MQ=60)

               TTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCAT                                                                                <  SRR4302547.5920305/94‑1 (MQ=60)

                TTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATT                                                                               <  SRR4302547.6034066/94‑1 (MQ=60)

                 TACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTC                                                                              <  SRR4302547.1365668/94‑1 (MQ=60)

                 TACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTC                                                                              >  SRR4302547.1653737/1‑94 (MQ=60)

                   CCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCAC                                                                            >  SRR4302547.5690118/1‑94 (MQ=60)

                     GTCTGTAAGTCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCG                                                                          <  SRR4302547.3046789/94‑1 (MQ=60)

                     GTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCG                                                                          >  SRR4302547.6746650/1‑94 (MQ=60)

                     GTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCG                                                                          <  SRR4302547.1138790/94‑1 (MQ=60)

                     GTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCG                                                                          >  SRR4302547.72392/1‑94 (MQ=60)

                     GTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCG                                                                          <  SRR4302547.3828948/94‑1 (MQ=60)

                     GTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCG                                                                          <  SRR4302547.2616985/94‑1 (MQ=60)

                      TCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGT                                                                         >  SRR4302547.50769/1‑94 (MQ=60)

                      TCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGT                                                                         <  SRR4302547.4853077/94‑1 (MQ=60)

                       CTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTT                                                                        <  SRR4302547.3923252/94‑1 (MQ=60)

                       CTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTT                                                                        <  SRR4302547.2125950/94‑1 (MQ=60)

                        TGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTA                                                                       <  SRR4302547.3661187/94‑1 (MQ=60)

                           AAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATT                                                                    >  SRR4302547.2074293/1‑94 (MQ=60)

                            AGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTC                                                                   <  SRR4302547.716067/94‑1 (MQ=60)

                             GGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCA                                                                  <  SRR4302547.2663113/94‑1 (MQ=60)

                               CCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAAT                                                                <  SRR4302547.1388164/94‑1 (MQ=60)

                               CCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAAT                                                                >  SRR4302547.5780461/1‑94 (MQ=60)

                                CGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATT                                                               <  SRR4302547.6373454/94‑1 (MQ=60)

                                CGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATT                                                               >  SRR4302547.1274037/1‑94 (MQ=60)

                                 GCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTT                                                              >  SRR4302547.5320968/1‑94 (MQ=60)

                                  CCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTT                                                             >  SRR4302547.3950751/1‑94 (MQ=60)

                                   CAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTC                                                            >  SRR4302547.2063067/1‑94 (MQ=60)

                                   CAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTC                                                            >  SRR4302547.1893367/1‑94 (MQ=60)

                                    AACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCA                                                           <  SRR4302547.1036615/94‑1 (MQ=60)

                                     ACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCAC                                                          <  SRR4302547.6126104/94‑1 (MQ=60)

                                     ACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCAC                                                          <  SRR4302547.3625168/94‑1 (MQ=60)

                                     ACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCAC                                                          <  SRR4302547.1935114/94‑1 (MQ=60)

                                     ACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCAC                                                          <  SRR4302547.40441/94‑1 (MQ=60)

                                      CATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACG                                                         >  SRR4302547.4944138/1‑94 (MQ=60)

                                       ATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGG                                                        >  SRR4302547.2773637/1‑94 (MQ=60)

                                       ATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGG                                                        >  SRR4302547.1110764/1‑94 (MQ=60)

                                         GTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCG                                                      <  SRR4302547.4808167/94‑1 (MQ=60)

                                          TGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGT                                                     <  SRR4302547.3023342/94‑1 (MQ=60)

                                          TGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGT                                                     <  SRR4302547.4333872/94‑1 (MQ=60)

                                           GCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTT                                                    <  SRR4302547.4657417/94‑1 (MQ=60)

                                            CAGCAACGGTTTATGTTCATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTA                                                   <  SRR4302547.708174/94‑1 (MQ=60)

                                            CAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTA                                                   <  SRR4302547.1806798/94‑1 (MQ=60)

                                            CAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTA                                                   >  SRR4302547.4392975/1‑94 (MQ=60)

                                            CAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTA                                                   >  SRR4302547.2213160/1‑94 (MQ=60)

                                             AGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAA                                                  >  SRR4302547.3260226/1‑94 (MQ=60)

                                              GCATCGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAAT                                                 <  SRR4302547.3728878/94‑1 (MQ=60)

                                               CAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCCTTCACCGTTAATTCAATTTTCACGGCGTTAACT                                                >  SRR4302547.4499453/1‑94 (MQ=60)

                                                AACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTG                                               >  SRR4302547.4436707/1‑94 (MQ=60)

                                                AACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTC                                               >  SRR4302547.1675026/1‑94 (MQ=60)

                                                AACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTC                                               >  SRR4302547.5870043/1‑94 (MQ=60)

                                                 ACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCC                                              <  SRR4302547.3688164/94‑1 (MQ=60)

                                                 ACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCC                                              >  SRR4302547.1090384/1‑94 (MQ=60)

                                                 ACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCC                                              <  SRR4302547.246984/94‑1 (MQ=60)

                                                  CGGTTTATGTACATTTTCACTCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCCTTTACCGTTGATTCAATTTTCACGGCGTTAATTACT                                             >  SRR4302547.5491279/1‑94 (MQ=60)

                                                  CGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCT                                             >  SRR4302547.1236629/1‑94 (MQ=60)

                                                   GGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTT                                            <  SRR4302547.5638158/94‑1 (MQ=60)

                                                   GGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTT                                            <  SRR4302547.962398/94‑1 (MQ=60)

                                                   GGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTT                                            <  SRR4302547.2603672/94‑1 (MQ=60)

                                                     TTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTA                                          <  SRR4302547.3588037/94‑1 (MQ=60)

                                                      TTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAA                                         >  SRR4302547.5767791/1‑94 (MQ=60)

                                                      TTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAA                                         >  SRR4302547.4832731/1‑94 (MQ=60)

                                                       TATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAG                                        <  SRR4302547.4465852/94‑1 (MQ=60)

                                                       TATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAG                                        >  SRR4302547.2718531/1‑94 (MQ=60)

                                                        ATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGG                                       >  SRR4302547.894275/1‑94 (MQ=60)

                                                         TGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGT                                      >  SRR4302547.5829373/1‑94 (MQ=60)

                                                         TGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGT                                      <  SRR4302547.1079265/94‑1 (MQ=60)

                                                          GTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTG                                     >  SRR4302547.801668/1‑94 (MQ=60)

                                                          GTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTG                                     <  SRR4302547.3554223/94‑1 (MQ=60)

                                                            ACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGT                                   <  SRR4302547.540786/94‑1 (MQ=60)

                                                             CATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTC                                  <  SRR4302547.449193/94‑1 (MQ=60)

                                                                TTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTT                               >  SRR4302547.1877005/1‑94 (MQ=60)

                                                                 TTCAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTA                              >  SRR4302547.4348872/1‑94 (MQ=60)

                                                                   CAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACT                            <  SRR4302547.841385/94‑1 (MQ=60)

                                                                   CAATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACT                            <  SRR4302547.3059478/94‑1 (MQ=60)

                                                                    AATCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTG                           >  SRR4302547.1098506/1‑94 (MQ=60)

                                                                      TCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCC                         >  SRR4302547.4886613/1‑94 (MQ=60)

                                                                      TCTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCC                         >  SRR4302547.1425163/1‑94 (MQ=60)

                                                                       CTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCA                        >  SRR4302547.514675/1‑94 (MQ=60)

                                                                       CTCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCA                        >  SRR4302547.6697326/1‑94 (MQ=60)

                                                                        TCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAG                       >  SRR4302547.5548625/1‑94 (MQ=60)

                                                                        TCTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAG                       <  SRR4302547.2064345/94‑1 (MQ=60)

                                                                         CTTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATGCCTTTAAGGTGGTCGTTACTGCCAGC                      >  SRR4302547.5471515/1‑94 (MQ=60)

                                                                          TTCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCG                     >  SRR4302547.1835762/1‑94 (MQ=60)

                                                                           TCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGT                    >  SRR4302547.3602753/1‑94 (MQ=60)

                                                                           TCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGT                    >  SRR4302547.2697574/1‑94 (MQ=60)

                                                                           TCGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGT                    <  SRR4302547.3701784/94‑1 (MQ=60)

                                                                            CGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTC                   <  SRR4302547.1256162/94‑1 (MQ=60)

                                                                            CGTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTC                   <  SRR4302547.1945383/94‑1 (MQ=60)

                                                                             GTTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCA                  >  SRR4302547.1593184/1‑94 (MQ=60)

                                                                             GTTCTGGAACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGGCAGCGTCA                  >  SRR4302547.5311889/1‑94 (MQ=60)

                                                                             GTNCTGGNNNNNTGCCCGTATNTNNNNNNNATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGNTCNNTNCTGCCAGCGTNA                  <  SRR4302547.6054955/94‑1 (MQ=60)

                                                                              TTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAG                 <  SRR4302547.3258142/94‑1 (MQ=60)

                                                                              TTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAG                 >  SRR4302547.6294801/1‑94 (MQ=60)

                                                                              TTCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAG                 <  SRR4302547.5499331/94‑1 (MQ=60)

                                                                               TCTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGC                <  SRR4302547.4384773/94‑1 (MQ=60)

                                                                                CTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCG               <  SRR4302547.5070862/94‑1 (MQ=60)

                                                                                CTGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCG               >  SRR4302547.5166931/1‑94 (MQ=60)

                                                                                 TGGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGC              <  SRR4302547.6183597/94‑1 (MQ=60)

                                                                                  GGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCC             <  SRR4302547.2327063/94‑1 (MQ=60)

                                                                                  GGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCC             <  SRR4302547.4644563/94‑1 (MQ=60)

                                                                                  GGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCC             <  SRR4302547.1096449/94‑1 (MQ=60)

                                                                                  GGTACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCC             <  SRR4302547.1768869/94‑1 (MQ=60)

                                                                                     ACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTT          <  SRR4302547.5536205/94‑1 (MQ=60)

                                                                                     ACTGTGCCCGTATCTTCAGCCCATTCGCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTT          <  SRR4302547.5842593/94‑1 (MQ=60)

                                                                                     ACTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTT          >  SRR4302547.5791025/1‑94 (MQ=60)

                                                                                      CTGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTT         >  SRR4302547.478288/1‑94 (MQ=60)

                                                                                       TGTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTT        >  SRR4302547.4900666/1‑94 (MQ=60)

                                                                                        GTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTG       <  SRR4302547.345147/94‑1 (MQ=60)

                                                                                        GTGCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTG       >  SRR4302547.6409995/1‑94 (MQ=60)

                                                                                          GCCCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGAC     >  SRR4302547.1125149/1‑94 (MQ=60)

                                                                                            CCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCA   >  SRR4302547.5876715/1‑94 (MQ=60)

                                                                                            CCGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCA   >  SRR4302547.1892008/1‑94 (MQ=60)

                                                                                             CGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCAT  >  SRR4302547.1326069/1‑94 (MQ=60)

                                                                                             CGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCAT  >  SRR4302547.616077/1‑94 (MQ=60)

GTACGCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCAT  >  NC_000913/3072579‑3072765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`