Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,143,485	T→C	K264K (AAA→AAG)	b2997 ←	putative hydrogenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,143,485	0	T	C	86.6%	712.3 / 92.6	269	K264K (AAA→AAG)	b2997	putative hydrogenase subunit
Reads supporting (aligned to +/- strand): ref base T (19/17); new base C (140/93); total (159/110)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-02

Only 100 of 277 total aligned reads displayed.

AGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGAC  >  NC_000913/3143394‑3143576

aGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCtt                                                                                            <  2:2153201/93‑1 (MQ=255)

 gCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCctt                                                                                           <  2:2231380/93‑1 (MQ=255)

 gCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCctt                                                                                           >  2:5809734/1‑93 (MQ=255)

 gCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCctt                                                                                           <  2:2913199/93‑1 (MQ=255)

 gCCATAGCAAGGGTGACCAATCCCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCctt                                                                                           <  1:5392777/93‑1 (MQ=255)

  ccATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTa                                                                                          <  1:5135793/93‑1 (MQ=255)

  ccATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTa                                                                                          >  1:5665470/1‑93 (MQ=255)

   cATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTAc                                                                                         >  1:2881442/1‑93 (MQ=255)

   cATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTAc                                                                                         <  2:1742704/93‑1 (MQ=255)

    aTAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACa                                                                                        <  2:2176321/93‑1 (MQ=255)

      aGCAAGGGTGACCAAGCGCCACCGGCCACACGCCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGc                                                                                      <  2:657158/93‑1 (MQ=255)

       gCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGcc                                                                                     >  1:2773971/1‑93 (MQ=255)

        cAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCg                                                                                    >  2:4863577/1‑93 (MQ=255)

         aaGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCgg                                                                                   >  2:2254444/1‑92 (MQ=255)

         aaGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGa                                                                                   >  2:6625215/1‑93 (MQ=255)

          aGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAg                                                                                  >  1:1119575/1‑93 (MQ=255)

          aGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAg                                                                                  >  2:6053574/1‑93 (MQ=255)

           gggTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAgg                                                                                 <  1:1538376/93‑1 (MQ=255)

           gggTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAgg                                                                                 >  1:6651769/1‑93 (MQ=255)

           gggTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAgg                                                                                 <  1:862530/93‑1 (MQ=255)

            ggTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAggt                                                                                <  2:3945217/93‑1 (MQ=255)

             gTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAggtg                                                                               >  2:1954745/1‑93 (MQ=255)

              tGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCCGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAggtgg                                                                              >  1:1706397/1‑93 (MQ=255)

               gACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAggtggt                                                                             >  2:3429541/1‑93 (MQ=255)

                acaaaTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCGGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTa                                                                            <  1:2644152/90‑1 (MQ=255)

                aCCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTa                                                                            <  1:4581801/93‑1 (MQ=255)

                 ccAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTAc                                                                           >  1:1043913/1‑93 (MQ=255)

                  cAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACa                                                                          >  1:1821746/1‑93 (MQ=255)

                  cAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACa                                                                          >  1:2072224/1‑93 (MQ=255)

                  cAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACa                                                                          >  1:4501274/1‑93 (MQ=255)

                   aaTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCCGCGTTGAGCAGTTGCCGTAAGTTTCTGGCGCCTTACAGCCGAGGCGGTAccg                                                                         >  2:2827082/1‑91 (MQ=255)

                   aaTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAg                                                                         >  1:502375/1‑93 (MQ=255)

                    aTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTa                                                                            <  2:844653/89‑1 (MQ=255)

                    aTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAgg                                                                        >  2:3240244/1‑93 (MQ=255)

                    aTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAgg                                                                        <  2:2277377/93‑1 (MQ=255)

                    aTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAgg                                                                        >  1:3509272/1‑93 (MQ=255)

                    aTCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAgg                                                                        >  1:4039832/1‑93 (MQ=255)

                      cGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCa                                                                      >  2:2849870/1‑93 (MQ=255)

                        ccACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCAcc                                                                    <  2:4050035/93‑1 (MQ=255)

                        ccACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCAcc                                                                    >  2:6227521/1‑93 (MQ=255)

                        ccACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCAcc                                                                    >  1:2050434/1‑93 (MQ=255)

                        ccACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCAcc                                                                    >  1:2231506/1‑93 (MQ=255)

                            cGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGcc                                                                <  2:1000249/93‑1 (MQ=255)

                            cGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGcc                                                                >  1:4685507/1‑93 (MQ=255)

                             ggCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCt                                                               <  1:3176537/93‑1 (MQ=255)

                             ggCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCt                                                               >  2:1896652/1‑93 (MQ=255)

                             ggCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCt                                                               >  1:6248418/1‑93 (MQ=255)

                             cgCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCt                                                               <  2:1221918/92‑1 (MQ=255)

                                 acacacCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTcgc                                                           >  1:2363210/1‑93 (MQ=255)

                                 acacacCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTcgc                                                           >  1:2296298/1‑93 (MQ=255)

                                  cacacCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTcgcg                                                          >  2:3339935/1‑93 (MQ=255)

                                    cacCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCGCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCggg                                                        >  2:6451604/1‑92 (MQ=255)

                                    cacCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGt                                                        <  1:3659271/93‑1 (MQ=255)

                                     acCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTg                                                       <  2:3400141/93‑1 (MQ=255)

                                      ccGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTgg                                                      >  1:1374882/1‑93 (MQ=255)

                                       cGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGc                                                     >  1:4152736/1‑93 (MQ=255)

                                       cGCCAAAATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACAAGCCTTCGCGGTGGc                                                     >  2:1627749/1‑93 (MQ=255)

                                         ccAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCt                                                   <  2:1227140/93‑1 (MQ=255)

                                          cAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCtt                                                  >  2:5138566/1‑93 (MQ=255)

                                           aaCATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTtc                                                 >  2:4580463/1‑93 (MQ=255)

                                           aaCATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTtc                                                 >  2:4142511/1‑93 (MQ=255)

                                             cATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTtcat                                               <  2:5640971/93‑1 (MQ=255)

                                                cGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCAc                                            <  1:768699/93‑1 (MQ=255)

                                                  cAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTTGCCCCTTACAGCCGAGGTGGTACAGGCACCTGCCTTCGCGGTGGCCCTCATCACCg                                          >  1:2814633/1‑93 (MQ=255)

                                                  cAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCg                                          >  2:436003/1‑93 (MQ=255)

                                                  cAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCg                                          >  1:3030611/1‑93 (MQ=255)

                                                   aGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCCCCGa                                         >  1:4747203/1‑93 (MQ=255)

                                                     aaTTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAAc                                       >  2:2423105/1‑93 (MQ=255)

                                                     aaTTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAAc                                       <  1:5827801/93‑1 (MQ=255)

                                                     aaTTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAAc                                       <  1:679971/93‑1 (MQ=255)

                                                      aTTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAAct                                      >  1:6553430/1‑93 (MQ=255)

                                                       ttGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAActc                                     >  2:6097132/1‑93 (MQ=255)

                                                       ttGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAActc                                     >  1:5942165/1‑93 (MQ=255)

                                                        tGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAActct                                    <  2:5463089/93‑1 (MQ=255)

                                                          cAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCttt                                  <  1:4376576/93‑1 (MQ=255)

                                                           aGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTg                                 >  2:5318839/1‑93 (MQ=255)

                                                            gCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTgg                                >  2:2609870/1‑93 (MQ=255)

                                                             cGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGc                               >  2:1170227/1‑93 (MQ=255)

                                                               ttGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCaa                             >  1:4894694/1‑93 (MQ=255)

                                                                tGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCaaa                            >  1:3939605/1‑93 (MQ=255)

                                                                 gAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCaaaa                           <  1:4277894/93‑1 (MQ=255)

                                                                  aGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAAc                          <  2:3626776/93‑1 (MQ=255)

                                                                  aGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAAc                          <  1:3375344/93‑1 (MQ=255)

                                                                    cAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGa                        <  1:4455890/93‑1 (MQ=255)

                                                                      gTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGAcc                      >  2:4407388/1‑93 (MQ=255)

                                                                       ttGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCa                     >  1:4218014/1‑93 (MQ=255)

                                                                       ttGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCa                     <  1:6066612/93‑1 (MQ=255)

                                                                        tGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAg                    <  1:1145625/93‑1 (MQ=255)

                                                                         gCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGc                   <  2:5747242/93‑1 (MQ=255)

                                                                          ccGTAAGGTTCTGGGCCTTTTCAGCCGAGTTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCa                  <  2:5337241/93‑1 (MQ=255)

                                                                           cGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCAt                 <  2:6314969/93‑1 (MQ=255)

                                                                           cGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCAt                 <  2:3061397/93‑1 (MQ=255)

                                                                            gTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATc                >  1:3868862/1‑93 (MQ=255)

                                                                             tAAGTTTCTGGCCCCTTACAGCCGAGGAGGTAAAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGACCTCTTTGGCAAAACGAACAGCATCg               >  1:2566614/1‑93 (MQ=255)

                                                                              aaGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGa              <  2:3832540/93‑1 (MQ=255)

                                                                                  ttCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTg          >  2:3587083/1‑93 (MQ=255)

                                                                                  ttCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTg          <  1:1623232/93‑1 (MQ=255)

                                                                                     tGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCgg       >  2:3071374/1‑93 (MQ=255)

                                                                                       gCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGc     >  1:6360153/1‑93 (MQ=255)

                                                                                       gCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGc     >  2:2899509/1‑93 (MQ=255)

AGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGAC  >  NC_000913/3143394‑3143576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGT  >  NC_000913/3143392‑3143578

ACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCC                                                                                               <  SRR4302547.5370874/94‑1 (MQ=60)

ACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCC                                                                                               >  SRR4302547.3877762/1‑94 (MQ=60)

 CAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCT                                                                                              >  SRR4302547.3912608/1‑94 (MQ=60)

 CAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCT                                                                                              <  SRR4302547.2390004/94‑1 (MQ=60)

 CAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCT                                                                                              <  SRR4302547.2015195/94‑1 (MQ=60)

 CAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCT                                                                                              >  SRR4302547.6074400/1‑94 (MQ=60)

 CAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCT                                                                                              <  SRR4302547.6166890/94‑1 (MQ=60)

   GCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTA                                                                                            <  SRR4302547.3999320/94‑1 (MQ=60)

   GCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTA                                                                                            >  SRR4302547.5782447/1‑94 (MQ=60)

   GCCATAGCAAGGGTGACCAATCCCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTA                                                                                            <  SRR4302547.5504170/94‑1 (MQ=60)

    CCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTAC                                                                                           >  SRR4302547.2938956/1‑94 (MQ=60)

    CCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTAC                                                                                           <  SRR4302547.5241602/94‑1 (MQ=60)

      ATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAG                                                                                         >  SRR4302547.4680471/1‑94 (MQ=60)

       TAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGC                                                                                        <  SRR4302547.837064/94‑1 (MQ=60)

        AGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCC                                                                                       >  SRR4302547.2829533/1‑94 (MQ=60)

         GCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCG                                                                                      <  SRR4302547.2389140/94‑1 (MQ=60)

         GCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCG                                                                                      >  SRR4302547.286014/1‑94 (MQ=60)

          CAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGA                                                                                     >  SRR4302547.553552/1‑94 (MQ=60)

           AAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAG                                                                                    >  SRR4302547.5559551/1‑94 (MQ=60)

           AAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAG                                                                                    >  SRR4302547.6615096/1‑94 (MQ=60)

           AAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAG                                                                                    >  SRR4302547.6752175/1‑94 (MQ=60)

           AAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAG                                                                                    >  SRR4302547.1142141/1‑94 (MQ=60)

            AGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGG                                                                                   >  SRR4302547.6790437/1‑94 (MQ=60)

             GGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGT                                                                                  <  SRR4302547.1569001/94‑1 (MQ=60)

             GGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGT                                                                                  <  SRR4302547.879015/94‑1 (MQ=60)

               GTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCCGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGG                                                                                >  SRR4302547.1739967/1‑94 (MQ=60)

               GTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGG                                                                                >  SRR4302547.6524661/1‑94 (MQ=60)

               GTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGG                                                                                <  SRR4302547.2918469/94‑1 (MQ=60)

                 GACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTA                                                                              <  SRR4302547.2976117/94‑1 (MQ=60)

                  ACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTAC                                                                             <  SRR4302547.4676622/94‑1 (MQ=60)

                  ACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTAC                                                                             >  SRR4302547.1064519/1‑94 (MQ=60)

                  ACAAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCGGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTAC                                                                             <  SRR4302547.2697360/94‑1 (MQ=60)

                   CCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACA                                                                            <  SRR4302547.2223259/94‑1 (MQ=60)

                   CCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACA                                                                            >  SRR4302547.2113577/1‑94 (MQ=60)

                   CCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACA                                                                            >  SRR4302547.4594245/1‑94 (MQ=60)

                   CCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACA                                                                            >  SRR4302547.1857760/1‑94 (MQ=60)

                    CAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAG                                                                           >  SRR4302547.4501168/1‑94 (MQ=60)

                    CAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAG                                                                           >  SRR4302547.511520/1‑94 (MQ=60)

                     AATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAat                                                                          >  SRR4302547.860774/1‑92 (MQ=60)

                     AATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGG                                                                          >  SRR4302547.3580896/1‑94 (MQ=60)

                     AATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGG                                                                          >  SRR4302547.4121867/1‑94 (MQ=60)

                      ATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGC                                                                         >  SRR4302547.6055106/1‑94 (MQ=60)

                      ATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGC                                                                         >  SRR4302547.9762/1‑94 (MQ=60)

                       TCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCA                                                                        <  SRR4302547.1137672/94‑1 (MQ=60)

                        CGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCAC                                                                       >  SRR4302547.3488858/1‑94 (MQ=60)

                         GCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACC                                                                      >  SRR4302547.2091330/1‑94 (MQ=60)

                         GCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACC                                                                      >  SRR4302547.2276484/1‑94 (MQ=60)

                          CCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCA                                                                     <  SRR4302547.3176803/94‑1 (MQ=60)

                           CACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAG                                                                    >  SRR4302547.5074876/1‑94 (MQ=60)

                           CACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGGGGTACAGGCACCAG                                                                    >  SRR4302547.315561/1‑94 (MQ=60)

                            ACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGC                                                                   >  SRR4302547.43516/1‑94 (MQ=60)

                            ACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGC                                                                   >  SRR4302547.3547343/1‑94 (MQ=60)

                             CCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCC                                                                  <  SRR4302547.4366888/94‑1 (MQ=60)

                             CCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCC                                                                  >  SRR4302547.1148552/1‑94 (MQ=60)

                             CCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCC                                                                  >  SRR4302547.4782564/1‑94 (MQ=60)

                              CGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCT                                                                 <  SRR4302547.4816044/94‑1 (MQ=60)

                              CGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCT                                                                 >  SRR4302547.6378217/1‑94 (MQ=60)

                              CGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCT                                                                 <  SRR4302547.1813030/94‑1 (MQ=60)

                               GGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTT                                                                >  SRR4302547.1005448/1‑94 (MQ=60)

                               GGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTT                                                                >  SRR4302547.689260/1‑94 (MQ=60)

                               GGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTT                                                                <  SRR4302547.3239804/94‑1 (MQ=60)

                               GGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTT                                                                >  SRR4302547.6716665/1‑94 (MQ=60)

                               GGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTT                                                                <  SRR4302547.6245311/94‑1 (MQ=60)

                                GCCACACACCGCCACCATCGCAGAATTGCAGCGTTGCGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTC                                                               <  SRR4302547.4324520/94‑1 (MQ=60)

                                GCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTC                                                               >  SRR4302547.4276211/1‑94 (MQ=60)

                                GCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTC                                                               <  SRR4302547.5459139/94‑1 (MQ=60)

                                  CACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGC                                                             >  SRR4302547.2411118/1‑94 (MQ=60)

                                  CACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGC                                                             >  SRR4302547.2342826/1‑94 (MQ=60)

                                  CACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGC                                                             >  SRR4302547.3349476/1‑94 (MQ=60)

                                  CACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTCACAGCCGAGGTGGTACAGGCACCAGCCTTAGC                                                             >  SRR4302547.6084587/1‑94 (MQ=60)

                                   ACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCG                                                            <  SRR4302547.4969093/94‑1 (MQ=60)

                                    CACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGG                                                           >  SRR4302547.1611878/1‑94 (MQ=60)

                                     ACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGT                                                          <  SRR4302547.1832143/94‑1 (MQ=60)

                                     ACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGT                                                          <  SRR4302547.4192219/94‑1 (MQ=60)

                                      CACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTG                                                         <  SRR4302547.3733928/94‑1 (MQ=60)

                                      CACCGCCAACATCGCAGAATTGCAGCGCTGAGCAGTTGCCGTAAGTTTCTGGACCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTG                                                         >  SRR4302547.1106524/1‑94 (MQ=60)

                                       ACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGG                                                        <  SRR4302547.1954082/94‑1 (MQ=60)

                                       ACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGG                                                        >  SRR4302547.1402451/1‑94 (MQ=60)

                                        CCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGC                                                       <  SRR4302547.73813/94‑1 (MQ=60)

                                        CCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGC                                                       >  SRR4302547.4237323/1‑94 (MQ=60)

                                         CGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCC                                                      >  SRR4302547.447848/1‑94 (MQ=60)

                                          GCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCT                                                     >  SRR4302547.5162066/1‑94 (MQ=60)

                                          GCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCT                                                     <  SRR4302547.2639661/94‑1 (MQ=60)

                                           CCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTT                                                    >  SRR4302547.3639820/1‑94 (MQ=60)

                                            CAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTC                                                   >  SRR4302547.132063/1‑94 (MQ=60)

                                            CAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTC                                                   >  SRR4302547.151738/1‑94 (MQ=60)

                                             AACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCA                                                  <  SRR4302547.4174364/94‑1 (MQ=60)

                                             AACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCA                                                  >  SRR4302547.4841932/1‑94 (MQ=60)

                                             AACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGGTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCA                                                  >  SRR4302547.2332228/1‑94 (MQ=60)

                                              ACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCAT                                                 <  SRR4302547.5283861/94‑1 (MQ=60)

                                                ATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTTGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCA                                               >  SRR4302547.2287247/1‑94 (MQ=60)

                                                ATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCA                                               >  SRR4302547.5200296/1‑94 (MQ=60)

                                                  CGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACC                                             <  SRR4302547.783227/94‑1 (MQ=60)

                                                   GCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTTGCCCCTTACAGCCGAGGTGGTACAGGCACCTGCCTTCGCGGTGGCCCTCATCACCG                                            >  SRR4302547.2870938/1‑94 (MQ=60)

                                                   GCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCG                                            >  SRR4302547.3090878/1‑94 (MQ=60)

                                                    CAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCCCCGA                                           >  SRR4302547.4845575/1‑94 (MQ=60)

                                                     AGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAA                                          >  SRR4302547.6277400/1‑94 (MQ=60)

                                                       AATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACT                                        <  SRR4302547.5948407/94‑1 (MQ=60)

                                                       AATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACT                                        <  SRR4302547.2102513/94‑1 (MQ=60)

                                                       AATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACT                                        <  SRR4302547.692503/94‑1 (MQ=60)

                                                       AATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACT                                        >  SRR4302547.6689858/1‑94 (MQ=60)

                                                        ATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTC                                       >  SRR4302547.6065381/1‑94 (MQ=60)

                                                            CAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTG                                   <  SRR4302547.4466518/94‑1 (MQ=60)

                                                            CAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTG                                   <  SRR4302547.3957259/94‑1 (MQ=60)

                                                             AGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGG                                  >  SRR4302547.4025048/1‑94 (MQ=60)

                                                             AGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCCTTGG                                  <  SRR4302547.5048849/94‑1 (MQ=60)

                                                              GCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGC                                 <  SRR4302547.4632878/94‑1 (MQ=60)

                                                               CGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCA                                >  SRR4302547.2928528/1‑94 (MQ=60)

                                                                GTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAA                               >  SRR4302547.1665019/1‑94 (MQ=60)

                                                                GTTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAA                               >  SRR4302547.4996070/1‑94 (MQ=60)

                                                                 TTGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAA                              >  SRR4302547.4019710/1‑94 (MQ=60)

                                                                  TGAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAA                             <  SRR4302547.6186268/94‑1 (MQ=60)

                                                                   GAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAAC                            <  SRR4302547.4365754/94‑1 (MQ=60)

                                                                   GAGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCAACGAACTCTTTGGCAAAAC                            <  SRR4302547.6008097/94‑1 (MQ=60)

                                                                    AGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACG                           <  SRR4302547.3443916/94‑1 (MQ=60)

                                                                    AGCAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACG                           <  SRR4302547.4023579/94‑1 (MQ=60)

                                                                      CAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGAC                         <  SRR4302547.4702720/94‑1 (MQ=60)

                                                                      CAGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGAC                         <  SRR4302547.4547992/94‑1 (MQ=60)

                                                                       AGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACC                        >  SRR4302547.2706709/1‑94 (MQ=60)

                                                                       AGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACC                        >  SRR4302547.1705978/1‑94 (MQ=60)

                                                                       AGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACC                        <  SRR4302547.2035490/94‑1 (MQ=60)

                                                                       AGTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCATCATCACCGAACTCTTTTGCAAAACGACC                        >  SRR4302547.2817350/1‑94 (MQ=60)

                                                                        GTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCA                       >  SRR4302547.4304138/1‑94 (MQ=60)

                                                                        GTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCA                       <  SRR4302547.282715/94‑1 (MQ=60)

                                                                        GTTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCA                       <  SRR4302547.6790932/94‑1 (MQ=60)

                                                                         TTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAG                      <  SRR4302547.6192442/94‑1 (MQ=60)

                                                                         TTGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAG                      <  SRR4302547.2138052/94‑1 (MQ=60)

                                                                          TGCCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGC                     <  SRR4302547.1168785/94‑1 (MQ=60)

                                                                            CCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCAT                   >  SRR4302547.2615589/1‑94 (MQ=60)

                                                                            CCGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCAT                   >  SRR4302547.647349/1‑94 (MQ=60)

                                                                             CGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATC                  >  SRR4302547.2367259/1‑94 (MQ=60)

                                                                             CGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATC                  >  SRR4302547.4451254/1‑94 (MQ=60)

                                                                             CGTAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATC                  >  SRR4302547.3947622/1‑94 (MQ=60)

                                                                              GTAAGTTTCTGGCCCCTTACAGCCGAGGAGGTAAAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGACCTCTTTGGCAAAACGAACAGCATCG                 >  SRR4302547.2618456/1‑94 (MQ=60)

                                                                               TAAGTTTCTGGCCCCTTACAGCCGAGGTGGTCCAGGCACCAGCCTTCGCGGTGGCCTTCATCAGCGAACTCTTTGGCCAAGCGACCAGCCTCGA                >  SRR4302547.4613552/1‑94 (MQ=60)

                                                                               TAAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGA                <  SRR4302547.242255/94‑1 (MQ=60)

                                                                                AAGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAA               <  SRR4302547.5844893/94‑1 (MQ=60)

                                                                                 AGTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTTGCAAAACGACCAGCATCGAAG              <  SRR4302547.377075/94‑1 (MQ=60)

                                                                                  GTTTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGT             <  SRR4302547.1359362/94‑1 (MQ=60)

                                                                                    TTCTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGC           <  SRR4302547.1655349/94‑1 (MQ=60)

                                                                                      CTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGG         <  SRR4302547.456659/94‑1 (MQ=60)

                                                                                      CTGGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGG         <  SRR4302547.3303611/94‑1 (MQ=60)

                                                                                       TGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGG        <  SRR4302547.144282/94‑1 (MQ=60)

                                                                                        GGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGC       >  SRR4302547.6492812/1‑94 (MQ=60)

                                                                                        GGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGC       <  SRR4302547.3779781/94‑1 (MQ=60)

                                                                                        GGCCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGC       >  SRR4302547.1161229/1‑94 (MQ=60)

                                                                                          CCCCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGA     >  SRR4302547.1279127/1‑94 (MQ=60)

                                                                                            CCTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACG   >  SRR4302547.2240868/1‑94 (MQ=60)

                                                                                             CTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGT  >  SRR4302547.6087151/1‑94 (MQ=60)

                                                                                             CTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGT  >  SRR4302547.1800683/1‑94 (MQ=60)

ACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCAGCGTTGAGCAGTTGCCGTAAGTTTCTGGCCCTTTACAGCCGAGGTGGTACAGGCACCAGCCTTCGCGGTGGCCTTCATCACCGAACTCTTTGGCAAAACGACCAGCATCGAAGTGCGGGCGACGT  >  NC_000913/3143392‑3143578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`