Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,333,863 A→G R91R (CGT→CGC)  murA ← UDP‑N‑glucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,333,8630AG100.0% 860.3 / NA 236R91R (CGT→CGC) murAUDP‑N‑glucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (125/111);  total (125/111)

Only 100 of 248 total aligned reads displayed.

CGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGC  >  NC_000913/3333773‑3333952
                                                                                          |                                                                                         
cGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGcgcg                                                                                         >  1:4721877/1‑93 (MQ=255)
cGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGcgcg                                                                                         <  2:2624693/93‑1 (MQ=255)
cGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGcgcg                                                                                         >  2:6508737/1‑93 (MQ=255)
 ggACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAgcgcgc                                                                                        >  2:2995283/1‑93 (MQ=255)
    cgcgCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATg                                                                                     <  2:6093539/93‑1 (MQ=255)
     gcgcACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATgg                                                                                    >  2:4385228/1‑93 (MQ=255)
     gcgcACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATgg                                                                                    <  1:6425738/93‑1 (MQ=255)
      cgcACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGt                                                                                   <  1:902453/93‑1 (MQ=255)
       gcACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGtt                                                                                  <  1:4187927/93‑1 (MQ=255)
        cACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGttt                                                                                 <  2:3702798/93‑1 (MQ=255)
        cACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGttt                                                                                 >  2:1613624/1‑93 (MQ=255)
          ccGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTa                                                                               >  2:3486057/1‑93 (MQ=255)
          ccGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTa                                                                               >  1:4644363/1‑93 (MQ=255)
             aTCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAAcc                                                                            >  1:511913/1‑93 (MQ=255)
              tCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCa                                                                           <  1:620796/93‑1 (MQ=255)
              tCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCa                                                                           <  2:6335870/93‑1 (MQ=255)
               cGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAg                                                                          <  2:4513543/93‑1 (MQ=255)
                 tACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGAt                                                                        >  2:5659980/1‑93 (MQ=255)
                 tACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGAt                                                                        <  1:6248594/93‑1 (MQ=255)
                  aCAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATc                                                                       >  2:5203337/1‑93 (MQ=255)
                     aCCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTa                                                                    >  2:4501620/1‑93 (MQ=255)
                     aCCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTa                                                                    <  1:2001196/93‑1 (MQ=255)
                     aCCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTa                                                                    >  1:1680870/1‑93 (MQ=255)
                     aCCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTa                                                                    >  2:5936368/1‑93 (MQ=255)
                      ccGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTaa                                                                   <  2:1549618/93‑1 (MQ=255)
                        gCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAgg                                                                 >  2:3072937/1‑93 (MQ=255)
                        gCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAgg                                                                 <  1:3087052/93‑1 (MQ=255)
                          cAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTg                                                               >  1:2742520/1‑93 (MQ=255)
                           aGGTAGTGAAACTTGCCCCTCACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTgc                                                              >  1:4400324/1‑93 (MQ=255)
                            ggTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTgcg                                                             >  2:2793251/1‑93 (MQ=255)
                             gTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTgcgc                                                            >  2:3776714/1‑93 (MQ=255)
                               aGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAg                                                          <  2:2069106/93‑1 (MQ=255)
                                gTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGa                                                         >  2:6520721/1‑93 (MQ=255)
                                  gAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAAt                                                       >  2:217355/1‑93 (MQ=255)
                                  gAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAAt                                                       >  1:3957731/1‑93 (MQ=255)
                                   aaaCTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATa                                                      >  1:6577591/1‑93 (MQ=255)
                                    aaCTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGAGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATAc                                                     >  1:4163299/1‑93 (MQ=255)
                                     aCTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACa                                                    <  2:6284500/93‑1 (MQ=255)
                                     aCTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACa                                                    >  1:1915282/1‑93 (MQ=255)
                                     aCTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACa                                                    <  2:4726472/93‑1 (MQ=255)
                                     aCTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACa                                                    >  2:5037567/1‑93 (MQ=255)
                                      cTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACAt                                                   <  2:2216187/93‑1 (MQ=255)
                                      cTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACAt                                                   <  1:1882102/93‑1 (MQ=255)
                                      cTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACAt                                                   <  1:2416713/93‑1 (MQ=255)
                                       ttGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACAtt                                                  <  1:2578257/93‑1 (MQ=255)
                                       ttGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACAtt                                                  >  1:5514257/1‑93 (MQ=255)
                                       ttGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACAtt                                                  >  1:3187259/1‑93 (MQ=255)
                                       ttGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACAtt                                                  >  2:4836528/1‑93 (MQ=255)
                                        tGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTa                                                 <  1:2248474/93‑1 (MQ=255)
                                          ccccTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAAc                                               <  2:331999/93‑1 (MQ=255)
                                           cccTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACg                                              >  2:5305980/1‑93 (MQ=255)
                                            ccTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGt                                             >  1:3428885/1‑93 (MQ=255)
                                             cTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTc                                            <  2:3412331/93‑1 (MQ=255)
                                             cTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTc                                            >  1:5839205/1‑93 (MQ=255)
                                               gACCAAAGCGCGCTACCAGCGGCCCCAGCTCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTcgc                                          <  2:1081394/93‑1 (MQ=255)
                                                aCCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTcgcg                                         <  2:6074935/93‑1 (MQ=255)
                                                 ccAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCgg                                        <  2:5460877/93‑1 (MQ=255)
                                                 ccAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCgg                                        <  2:6215729/93‑1 (MQ=255)
                                                 ccAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCgg                                        >  1:2083287/1‑93 (MQ=255)
                                                 ccAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCgg                                        <  1:6507658/93‑1 (MQ=255)
                                                   aaaGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGACCGTAAGGTGCGCAGAATACATTAACGTCGCGGGc                                      >  2:2334397/1‑93 (MQ=255)
                                                    aaGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCa                                     >  1:5942089/1‑93 (MQ=255)
                                                    aaGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCa                                     >  2:1980556/1‑93 (MQ=255)
                                                    aaGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCa                                     <  1:1510663/93‑1 (MQ=255)
                                                    aaGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCa                                     >  2:150421/1‑93 (MQ=255)
                                                     aGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCAt                                    <  1:2494360/93‑1 (MQ=255)
                                                     aGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCAt                                    >  2:5883603/1‑93 (MQ=255)
                                                      gcgcgcTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATc                                   <  1:2869040/93‑1 (MQ=255)
                                                        gcgcTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCaa                                 >  1:1033788/1‑93 (MQ=255)
                                                        gcgcTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCaa                                 <  1:4299696/93‑1 (MQ=255)
                                                         cgcTACCAGCGGCCCCAGAGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAat                                <  1:5766272/93‑1 (MQ=255)
                                                          gcTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAata                               <  1:1605474/93‑1 (MQ=255)
                                                            tACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGGGCGCAGAAGACATTAACGTCGCGGGCATCAATATg                             >  2:726341/1‑93 (MQ=255)
                                                               cAGCGGCCCCAGAGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGcac                          >  2:4134616/1‑93 (MQ=255)
                                                                 gCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAg                        >  2:2943729/1‑93 (MQ=255)
                                                                    gCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAAc                     <  2:1797807/93‑1 (MQ=255)
                                                                    gCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAAc                     >  2:136653/1‑93 (MQ=255)
                                                                      cccAGCCCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGAGCAGAATACATTATCGTCGCGGGCATCAATATGCACAGACCCa                   <  1:5625186/93‑1 (MQ=255)
                                                                       ccAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCAt                  >  2:2575943/1‑93 (MQ=255)
                                                                         aGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTa                <  2:4361860/93‑1 (MQ=255)
                                                                         aGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTa                <  1:1224462/93‑1 (MQ=255)
                                                                          gcgcCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAc               <  1:387864/93‑1 (MQ=255)
                                                                          gcgcCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAc               >  1:3546546/1‑93 (MQ=255)
                                                                           cgcCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACg              <  1:4030161/93‑1 (MQ=255)
                                                                           cgcCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACg              >  1:1089930/1‑93 (MQ=255)
                                                                            gcCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGt             <  1:4065318/93‑1 (MQ=255)
                                                                            gcCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGt             <  1:3729778/93‑1 (MQ=255)
                                                                             cccAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGtt            <  1:725070/93‑1 (MQ=255)
                                                                             cccAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGtt            >  2:791552/1‑93 (MQ=255)
                                                                              ccAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTc           >  1:5385101/1‑93 (MQ=255)
                                                                              ccAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTc           <  1:4959297/93‑1 (MQ=255)
                                                                               cAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCt          >  1:3302263/1‑93 (MQ=255)
                                                                                 gATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTAc        <  2:1658837/93‑1 (MQ=255)
                                                                                 gATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAAAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTAc        >  1:3580686/1‑93 (MQ=255)
                                                                                   tAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACtt      <  2:184832/93‑1 (MQ=255)
                                                                                   tAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACtt      >  1:1423756/1‑93 (MQ=255)
                                                                                    aGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACttt     <  2:5336187/93‑1 (MQ=255)
                                                                                    aGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACttt     <  2:2457606/93‑1 (MQ=255)
                                                                                     gAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTc    >  2:3155531/1‑93 (MQ=255)
                                                                                     gAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTc    <  2:6426955/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGC  >  NC_000913/3333773‑3333952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGC  >  NC_000913/3333770‑3333952
                                                                                             |                                                                                         
AACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCG                                                                                           >  SRR4302547.1049906/1‑94 (MQ=60)
AACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCG                                                                                           <  SRR4302547.3639026/94‑1 (MQ=60)
AACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCG                                                                                           >  SRR4302547.1782974/1‑94 (MQ=60)
 ACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGC                                                                                          <  SRR4302547.4817686/94‑1 (MQ=60)
 ACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGC                                                                                          >  SRR4302547.408176/1‑94 (MQ=60)
 ACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGC                                                                                          >  SRR4302547.6171981/1‑94 (MQ=60)
  CCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCG                                                                                         >  SRR4302547.4819652/1‑94 (MQ=60)
    GGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCA                                                                                       >  SRR4302547.6644231/1‑94 (MQ=60)
    GGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCA                                                                                       >  SRR4302547.1963979/1‑94 (MQ=60)
     GACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCAT                                                                                      <  SRR4302547.6367098/94‑1 (MQ=60)
        GCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGT                                                                                   <  SRR4302547.6559699/94‑1 (MQ=60)
         CGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTT                                                                                  <  SRR4302547.919821/94‑1 (MQ=60)
         CGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTT                                                                                  >  SRR4302547.4217167/1‑94 (MQ=60)
         CGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGGT                                                                                  >  SRR4302547.4409883/1‑94 (MQ=60)
          GCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTT                                                                                 <  SRR4302547.4273314/94‑1 (MQ=60)
           CACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTT                                                                                <  SRR4302547.720350/94‑1 (MQ=60)
            CCCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTA                                                                               <  SRR4302547.5892101/94‑1 (MQ=60)
            ACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTA                                                                               >  SRR4302547.4740549/1‑94 (MQ=60)
             CCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAA                                                                              >  SRR4302547.2841745/1‑94 (MQ=60)
               GATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACC                                                                            >  SRR4302547.521200/1‑94 (MQ=60)
                 TCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAG                                                                          <  SRR4302547.632279/94‑1 (MQ=60)
                 TCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAG                                                                          <  SRR4302547.5486923/94‑1 (MQ=60)
                  CGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGA                                                                         >  SRR4302547.5065762/1‑94 (MQ=60)
                  CGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGA                                                                         >  SRR4302547.3175701/1‑94 (MQ=60)
                   GTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGAT                                                                        <  SRR4302547.5551783/94‑1 (MQ=60)
                   GTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGAT                                                                        >  SRR4302547.5702236/1‑94 (MQ=60)
                    TACAACCGCGAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATC                                                                       <  SRR4302547.4075505/94‑1 (MQ=60)
                    TACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATC                                                                       <  SRR4302547.6378396/94‑1 (MQ=60)
                       AACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTA                                                                    >  SRR4302547.1713963/1‑94 (MQ=60)
                       AACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTA                                                                    >  SRR4302547.743849/1‑94 (MQ=60)
                        ACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAA                                                                   <  SRR4302547.2041018/94‑1 (MQ=60)
                           GCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGT                                                                <  SRR4302547.3546260/94‑1 (MQ=60)
                           GCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGT                                                                <  SRR4302547.326048/94‑1 (MQ=60)
                           GCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGT                                                                <  SRR4302547.3148305/94‑1 (MQ=60)
                            CCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTG                                                               <  SRR4302547.2461546/94‑1 (MQ=60)
                            CCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTG                                                               <  SRR4302547.4129763/94‑1 (MQ=60)
                            CCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTG                                                               <  SRR4302547.162470/94‑1 (MQ=60)
                            CCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTG                                                               >  SRR4302547.3006413/1‑94 (MQ=60)
                            CCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTG                                                               >  SRR4302547.2797529/1‑94 (MQ=60)
                             CAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGC                                                              <  SRR4302547.3470876/94‑1 (MQ=60)
                             CAGGTAGTGAAACTTGCCCCTCACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGC                                                              >  SRR4302547.4490936/1‑94 (MQ=60)
                              AGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCG                                                             >  SRR4302547.213002/1‑94 (MQ=60)
                                  AGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGA                                                         >  SRR4302547.3660592/1‑94 (MQ=60)
                                    TGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAAT                                                       >  SRR4302547.4038234/1‑94 (MQ=60)
                                     GAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATA                                                      >  SRR4302547.6714557/1‑94 (MQ=60)
                                      AAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATAC                                                     <  SRR4302547.2692164/94‑1 (MQ=60)
                                      AAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATAC                                                     <  SRR4302547.5860654/94‑1 (MQ=60)
                                      AAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGAGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATAC                                                     >  SRR4302547.4248102/1‑94 (MQ=60)
                                       AACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCGTGGTTTTAACCAGATCGTAAGGTGCGCAGAATACA                                                    >  SRR4302547.5972223/1‑94 (MQ=60)
                                       AACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACA                                                    >  SRR4302547.1953174/1‑94 (MQ=60)
                                       AACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACA                                                    <  SRR4302547.5449875/94‑1 (MQ=60)
                                         CTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATT                                                  >  SRR4302547.3250771/1‑94 (MQ=60)
                                         CTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATT                                                  <  SRR4302547.1919282/94‑1 (MQ=60)
                                         CTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATT                                                  >  SRR4302547.5628096/1‑94 (MQ=60)
                                         CTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATT                                                  <  SRR4302547.2465647/94‑1 (MQ=60)
                                          TTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTA                                                 <  SRR4302547.2630285/94‑1 (MQ=60)
                                          TTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTA                                                 <  SRR4302547.6091849/94‑1 (MQ=60)
                                           TGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAA                                                <  SRR4302547.2293863/94‑1 (MQ=60)
                                            GCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAAC                                               <  SRR4302547.2786626/94‑1 (MQ=60)
                                            GCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAAC                                               <  SRR4302547.4003112/94‑1 (MQ=60)
                                             CCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACG                                              <  SRR4302547.6653417/94‑1 (MQ=60)
                                              CCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGT                                             >  SRR4302547.3498738/1‑94 (MQ=60)
                                               CCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTC                                            >  SRR4302547.5960022/1‑94 (MQ=60)
                                               CCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTC                                            <  SRR4302547.4252747/94‑1 (MQ=60)
                                               CCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTC                                            >  SRR4302547.1654793/1‑94 (MQ=60)
                                                  GACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCG                                         >  SRR4302547.3631832/1‑94 (MQ=60)
                                                  GACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCG                                         <  SRR4302547.1370163/94‑1 (MQ=60)
                                                   ACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGG                                        >  SRR4302547.2124827/1‑94 (MQ=60)
                                                    CCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGG                                       <  SRR4302547.1454505/94‑1 (MQ=60)
                                                    CCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGG                                       <  SRR4302547.6643152/94‑1 (MQ=60)
                                                     CAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGC                                      <  SRR4302547.3347082/94‑1 (MQ=60)
                                                     CAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGC                                      >  SRR4302547.5952598/1‑94 (MQ=60)
                                                     CAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATCGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGCGC                                      >  SRR4302547.2327738/1‑94 (MQ=60)
                                                      AAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCA                                     >  SRR4302547.6065303/1‑94 (MQ=60)
                                                       AAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCAT                                    <  SRR4302547.1540768/94‑1 (MQ=60)
                                                        AGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATC                                   <  SRR4302547.2544758/94‑1 (MQ=60)
                                                         GCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCA                                  <  SRR4302547.2926351/94‑1 (MQ=60)
                                                          CGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAA                                 >  SRR4302547.1054125/1‑94 (MQ=60)
                                                           GCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAAT                                <  SRR4302547.4388111/94‑1 (MQ=60)
                                                           GCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAAT                                >  SRR4302547.6401446/1‑94 (MQ=60)
                                                           GCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAAT                                >  SRR4302547.4959796/1‑94 (MQ=60)
                                                           GCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTACGCAGAATACATAAACGTCGCGGGCATCAAT                                >  SRR4302547.4924576/1‑94 (MQ=60)
                                                            CGCTACCAGCGGCCCCAGAGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATA                               <  SRR4302547.5885527/94‑1 (MQ=60)
                                                             GCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATAT                              >  SRR4302547.1588610/1‑94 (MQ=60)
                                                             GCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATAT                              >  SRR4302547.1715780/1‑94 (MQ=60)
                                                             GCTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATAT                              <  SRR4302547.1637308/94‑1 (MQ=60)
                                                              CTACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATG                             >  SRR4302547.2490485/1‑94 (MQ=60)
                                                                ACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCA                           <  SRR4302547.2481180/94‑1 (MQ=60)
                                                                ACCAGCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCA                           >  SRR4302547.4508361/1‑94 (MQ=60)
                                                                    GCGGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCCGGCATCAATATGCACAGA                       >  SRR4302547.2184392/1‑94 (MQ=60)
                                                                      GGCCCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAAC                     <  SRR4302547.4007939/94‑1 (MQ=60)
                                                                      GGCCCCAGCACCCAGATAGATGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAAC                     >  SRR4302547.6599571/1‑94 (MQ=60)
                                                                         CCCAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCAT                  >  SRR4302547.3589613/1‑94 (MQ=60)
                                                                         CCCAGCCCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGAGCAGAATACATTATCGTCGCGGGCATCAATATGCACAGACCCAT                  <  SRR4302547.5741266/94‑1 (MQ=60)
                                                                           CAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTA                <  SRR4302547.4240494/94‑1 (MQ=60)
                                                                           CAGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTA                <  SRR4302547.192709/94‑1 (MQ=60)
                                                                            AGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAC               <  SRR4302547.2086759/94‑1 (MQ=60)
                                                                            AGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAC               <  SRR4302547.4382807/94‑1 (MQ=60)
                                                                            AGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAC               >  SRR4302547.3618928/1‑94 (MQ=60)
                                                                            AGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAC               <  SRR4302547.1249160/94‑1 (MQ=60)
                                                                            AGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAC               <  SRR4302547.3930523/94‑1 (MQ=60)
                                                                            AGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAC               <  SRR4302547.1127523/94‑1 (MQ=60)
                                                                            AGCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTAC               <  SRR4302547.4766807/94‑1 (MQ=60)
                                                                             GCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACG              <  SRR4302547.395056/94‑1 (MQ=60)
                                                                             GCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACG              <  SRR4302547.3099865/94‑1 (MQ=60)
                                                                             GCGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACG              >  SRR4302547.1111765/1‑94 (MQ=60)
                                                                              CGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGT             >  SRR4302547.2020535/1‑94 (MQ=60)
                                                                              CGCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGT             <  SRR4302547.4112027/94‑1 (MQ=60)
                                                                               GCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTT            <  SRR4302547.4147890/94‑1 (MQ=60)
                                                                               GCCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTT            <  SRR4302547.3805927/94‑1 (MQ=60)
                                                                                CCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTC           <  SRR4302547.738580/94‑1 (MQ=60)
                                                                                CCCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTC           >  SRR4302547.5496295/1‑94 (MQ=60)
                                                                                 CCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCT          >  SRR4302547.3368745/1‑94 (MQ=60)
                                                                                 CCAGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCT          >  SRR4302547.3664017/1‑94 (MQ=60)
                                                                                 CCAGATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCT          <  SRR4302547.5061918/94‑1 (MQ=60)
                                                                                   AGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTAC        >  SRR4302547.5685656/1‑94 (MQ=60)
                                                                                   AGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTAC        >  SRR4302547.4283825/1‑94 (MQ=60)
                                                                                   AGATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAAAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTAC        >  SRR4302547.3653735/1‑94 (MQ=60)
                                                                                    GATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACT       <  SRR4302547.6079161/94‑1 (MQ=60)
                                                                                    GATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACT       >  SRR4302547.4574470/1‑94 (MQ=60)
                                                                                     ATAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTT      >  SRR4302547.1452260/1‑94 (MQ=60)
                                                                                     ATAGAAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTT      >  SRR4302547.806463/1‑94 (MQ=60)
                                                                                      TAGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTT     <  SRR4302547.6431871/94‑1 (MQ=60)
                                                                                       AGAAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTC    >  SRR4302547.2586077/1‑94 (MQ=60)
                                                                                       AGAAGCGCGCATGGCTTTAACCAGATCGCAAGGGGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTC    >  SRR4302547.1090593/1‑94 (MQ=60)
                                                                                         AAGCGCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGC  >  SRR4302547.1370207/1‑94 (MQ=60)
                                                                                         AAGCGCGCATGGTTTTAACCAGATCGCAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGC  >  SRR4302547.1849004/1‑94 (MQ=60)
                                                                                             |                                                                                         
AACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGC  >  NC_000913/3333770‑3333952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑