Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,336,835	T→C	T19A (ACG→GCG)	yrbE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,336,835	0	T	C	83.3%	580.0 / 104.4	239	T19A (ACG→GCG)	yrbE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (16/24); new base C (94/105); total (110/129)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 243 total aligned reads displayed.

AGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGA  >  NC_000913/3336745‑3336924

agagCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCtc                                                                                         <  2:262304/93‑1 (MQ=255)

   gCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCa                                                                                      <  1:3803834/93‑1 (MQ=255)

   gCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCa                                                                                      <  1:4012865/93‑1 (MQ=255)

   gCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCa                                                                                      >  2:6273011/1‑93 (MQ=255)

    cTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAg                                                                                     <  2:4760091/93‑1 (MQ=255)

    cTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAg                                                                                     >  2:3998781/1‑93 (MQ=255)

      ggCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAggg                                                                                   >  2:4621603/1‑93 (MQ=255)

      ggCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAggg                                                                                   >  2:2327564/1‑93 (MQ=255)

        cgcACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGtt                                                                                 <  2:893625/93‑1 (MQ=255)

         gcACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGttt                                                                                <  2:5045662/93‑1 (MQ=255)

          cACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGtttt                                                                               <  1:103248/93‑1 (MQ=255)

          cACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGtttt                                                                               >  1:6545034/1‑93 (MQ=255)

            ccAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTaa                                                                             <  2:2697825/93‑1 (MQ=255)

              agcagcGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGGATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATc                                                                           >  2:75114/1‑93 (MQ=255)

              agcagcGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATc                                                                           <  2:2753978/93‑1 (MQ=255)

              agcagcGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATc                                                                           >  1:6315409/1‑93 (MQ=255)

               gcagcGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATcc                                                                          <  1:6613844/93‑1 (MQ=255)

               gcagcGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATcc                                                                          <  1:5924198/93‑1 (MQ=255)

                   cggcgCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCttt                                                                      <  1:3662838/93‑1 (MQ=255)

                   cggcgCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCttt                                                                      <  1:4658798/93‑1 (MQ=255)

                    ggcgCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTa                                                                     <  2:941983/93‑1 (MQ=255)

                    ggcgCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCACGCCCGAACGCTCTCAGGGTTTTAATCCCTTTa                                                                     >  2:1691294/1‑93 (MQ=255)

                     gcgcATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCTCGCCGCCCGAAGGCTCTCAGGGTTTTAATACCTTTAt                                                                    >  2:2151908/1‑93 (MQ=255)

                     gcgcATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTAt                                                                    <  1:1810106/93‑1 (MQ=255)

                      cgcATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATg                                                                   <  2:4938820/93‑1 (MQ=255)

                      cgcATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATg                                                                   <  2:3423614/93‑1 (MQ=255)

                        cATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTc                                                                 >  2:5970034/1‑93 (MQ=255)

                        cATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTc                                                                 >  1:3386438/1‑93 (MQ=255)

                         aTGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTcc                                                                <  2:6515444/93‑1 (MQ=255)

                          tGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCg                                                               >  1:4604111/1‑93 (MQ=255)

                          tGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCg                                                               >  1:4872614/1‑93 (MQ=255)

                           gTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGa                                                              <  2:2551170/93‑1 (MQ=255)

                                cGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGAc                                                         <  2:2865539/93‑1 (MQ=255)

                                 gAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGCTTTAATCCCTTTATGTCCGAGCGACg                                                        >  1:5634846/1‑93 (MQ=255)

                                  aaaTTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGc                                                       >  2:1094914/1‑93 (MQ=255)

                                    aTTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCa                                                     >  2:712484/1‑93 (MQ=255)

                                     ttCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAg                                                    >  1:583018/1‑93 (MQ=255)

                                     ttCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAg                                                    <  1:2985433/93‑1 (MQ=255)

                                     ttCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGGTTTAATCCCTTTATGTCCGAGCGACGCCAg                                                    >  1:4786385/1‑93 (MQ=255)

                                      tCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAgc                                                   <  2:36218/93‑1 (MQ=255)

                                      tCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAgc                                                   <  2:1131331/93‑1 (MQ=255)

                                         ggTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCa                                                >  2:6179911/1‑93 (MQ=255)

                                         ggTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCa                                                >  2:4299234/1‑93 (MQ=255)

                                           tttGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCAtt                                              <  2:5676937/93‑1 (MQ=255)

                                            ttGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCAttt                                             <  2:6065544/93‑1 (MQ=255)

                                            ttGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCAttt                                             >  1:2702543/1‑93 (MQ=255)

                                               ccGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAAc                                          <  1:4275158/93‑1 (MQ=255)

                                                cGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACa                                         >  1:1655877/1‑93 (MQ=255)

                                                  aCCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGc                                       >  2:3497021/1‑93 (MQ=255)

                                                  aCCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGc                                       >  1:4807303/1‑93 (MQ=255)

                                                   ccAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCa                                      >  1:774419/1‑93 (MQ=255)

                                                    cAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCAt                                     >  2:334411/1‑93 (MQ=255)

                                                     aGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATg                                    >  1:4367979/1‑93 (MQ=255)

                                                      gcgcATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGa                                   >  2:4585240/1‑93 (MQ=255)

                                                       cgcATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAg                                  >  2:3670049/1‑93 (MQ=255)

                                                       cgcATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAg                                  <  2:5845988/93‑1 (MQ=255)

                                                        gcATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGt                                 <  1:52490/93‑1 (MQ=255)

                                                         cATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTg                                >  1:122648/1‑93 (MQ=255)

                                                          aTTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTgg                               >  1:2275152/1‑93 (MQ=255)

                                                          aTTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTgg                               >  1:5516143/1‑93 (MQ=255)

                                                           ttGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGc                              <  2:1375207/93‑1 (MQ=255)

                                                           ttGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGc                              <  2:5578131/93‑1 (MQ=255)

                                                           ttGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGc                              <  1:198943/93‑1 (MQ=255)

                                                            tGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCt                             <  2:4450990/93‑1 (MQ=255)

                                                             gAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCtt                            <  2:6418214/93‑1 (MQ=255)

                                                                tAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAAc                         <  1:1384122/93‑1 (MQ=255)

                                                                tAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAAc                         <  2:5127508/93‑1 (MQ=255)

                                                                tAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAAc                         >  2:1474780/1‑93 (MQ=255)

                                                                  aCATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTc                       <  1:418549/93‑1 (MQ=255)

                                                                  aCATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTc                       >  1:5099182/1‑93 (MQ=255)

                                                                  aCATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTc                       <  2:2462542/93‑1 (MQ=255)

                                                                    aTTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTccc                     <  1:3195797/93‑1 (MQ=255)

                                                                     ttAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCt                    >  2:5435550/1‑93 (MQ=255)

                                                                     ttAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCt                    <  2:4137981/93‑1 (MQ=255)

                                                                     ttAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCt                    >  1:3606381/1‑93 (MQ=255)

                                                                      tAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTGTGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTg                   >  2:3551739/1‑93 (MQ=255)

                                                                       aaCCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTgg                  <  2:547040/93‑1 (MQ=255)

                                                                          ccGGCCCGCCCGACCGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTaa               <  2:3288163/93‑1 (MQ=255)

                                                                            ggCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTaaaa             >  1:4204871/1‑93 (MQ=255)

                                                                            ggCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTaaaa             <  2:2909349/93‑1 (MQ=255)

                                                                              cccgcccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGa           >  1:1740154/1‑93 (MQ=255)

                                                                               ccgcccgAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGAt          >  2:3199286/1‑93 (MQ=255)

                                                                               ccgcccgAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGAt          >  2:1552215/1‑93 (MQ=255)

                                                                               ccgcccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGAt          <  2:5362404/93‑1 (MQ=255)

                                                                               ccgcccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGAt          <  1:5899116/93‑1 (MQ=255)

                                                                                 gcccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCa        >  1:3905108/1‑93 (MQ=255)

                                                                                 gcccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCa        >  2:5214096/1‑93 (MQ=255)

                                                                                 gcccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCa        <  1:302173/93‑1 (MQ=255)

                                                                                  cccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAg       <  2:1987153/93‑1 (MQ=255)

                                                                                  cccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAg       <  1:6046649/93‑1 (MQ=255)

                                                                                  cccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAg       <  1:820445/93‑1 (MQ=255)

                                                                                   ccgAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGc      >  1:363086/1‑93 (MQ=255)

                                                                                   ccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGc      >  2:553779/1‑93 (MQ=255)

                                                                                   ccgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGc      <  1:2068842/93‑1 (MQ=255)

                                                                                    cgAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGct     <  2:6268118/93‑2 (MQ=255)

                                                                                     gAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGt    <  2:394863/93‑1 (MQ=255)

                                                                                      aaCGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTg   >  1:2762437/1‑93 (MQ=255)

                                                                                      aaCGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTg   >  1:934303/1‑93 (MQ=255)

                                                                                      aaCGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTg   >  2:4930864/1‑93 (MQ=255)

                                                                                       aCGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGa  >  1:1710594/1‑93 (MQ=255)

AGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGA  >  NC_000913/3336745‑3336924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAAT  >  NC_000913/3336742‑3336928

TATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGC                                                                                               <  SRR4302547.3828594/94‑1 (MQ=60)

 ATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCT                                                                                              <  SRR4302547.6335615/94‑1 (MQ=60)

   AGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCT                                                                                            >  SRR4302547.6081073/1‑94 (MQ=60)

     AGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCA                                                                                          >  SRR4302547.4283003/1‑94 (MQ=60)

      GCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAG                                                                                         <  SRR4302547.3881377/94‑1 (MQ=60)

      GCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAG                                                                                         <  SRR4302547.1661066/94‑1 (MQ=60)

      GCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAG                                                                                         <  SRR4302547.4094431/94‑1 (MQ=60)

      GCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAG                                                                                         >  SRR4302547.3841762/1‑94 (MQ=60)

       CTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGG                                                                                        >  SRR4302547.2864909/1‑94 (MQ=60)

       CTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGG                                                                                        >  SRR4302547.2534435/1‑94 (MQ=60)

       CTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTCCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGG                                                                                        <  SRR4302547.1767238/94‑1 (MQ=60)

         GGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGT                                                                                      <  SRR4302547.5712294/94‑1 (MQ=60)

           CGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTT                                                                                    >  SRR4302547.4079979/1‑94 (MQ=60)

            GCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTT                                                                                   >  SRR4302547.6681251/1‑94 (MQ=60)

             CACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTA                                                                                  >  SRR4302547.477817/1‑94 (MQ=60)

             CACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTA                                                                                  >  SRR4302547.3115624/1‑94 (MQ=60)

             CACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTA                                                                                  <  SRR4302547.105137/94‑1 (MQ=60)

               CCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAAT                                                                                >  SRR4302547.6493507/1‑94 (MQ=60)

               CCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAAT                                                                                <  SRR4302547.4547875/94‑1 (MQ=60)

                CAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATC                                                                               >  SRR4302547.6446732/1‑94 (MQ=60)

                CAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATC                                                                               >  SRR4302547.2925507/1‑94 (MQ=60)

                  GCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCC                                                                             <  SRR4302547.6046984/94‑1 (MQ=60)

                  GCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCC                                                                             <  SRR4302547.6751510/94‑1 (MQ=60)

                     GCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAAAGCTCTCATGGGTTTAATCCCTTT                                                                          >  SRR4302547.5076976/1‑94 (MQ=60)

                      CGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTA                                                                         <  SRR4302547.3737569/94‑1 (MQ=60)

                      CGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTA                                                                         <  SRR4302547.4755282/94‑1 (MQ=60)

                       GGTGCATGTTTGCGAAATTCCGGTTTGGCGACCAGCGCATTGAATAACATTAACCCGGACCGCCCGAACGCTCTCAGGGTTTTAATCCTTTTAT                                                                        >  SRR4302547.4081220/1‑94 (MQ=60)

                        GCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATG                                                                       <  SRR4302547.1845871/94‑1 (MQ=60)

                          GCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTC                                                                     <  SRR4302547.6279348/94‑1 (MQ=60)

                          GCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTC                                                                     >  SRR4302547.3455317/1‑94 (MQ=60)

                           CATGTTTGCGAAATTCCGGTTTGACGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCC                                                                    >  SRR4302547.1432023/1‑94 (MQ=60)

                            ATGTTTGCGAAATTCCGGTTTGGCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCG                                                                   >  SRR4302547.4316636/1‑94 (MQ=60)

                            ATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGGCCCAACGCCCCCAGGGGTTTAATCCCTCTATGTCTG                                                                   >  SRR4302547.387522/1‑94 (MQ=60)

                            ATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCG                                                                   >  SRR4302547.3064550/1‑94 (MQ=60)

                            ATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCG                                                                   >  SRR4302547.4699384/1‑94 (MQ=60)

                            ATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCG                                                                   >  SRR4302547.4973588/1‑94 (MQ=60)

                             TGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGA                                                                  <  SRR4302547.44138/94‑1 (MQ=60)

                             TGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGA                                                                  <  SRR4302547.3683787/94‑1 (MQ=60)

                             TGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGA                                                                  <  SRR4302547.508134/94‑1 (MQ=60)

                                  GCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGAC                                                             >  SRR4302547.1149303/1‑94 (MQ=60)

                                   CGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACG                                                            <  SRR4302547.1855868/94‑1 (MQ=60)

                                   CGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGCTTTAATCCCTTTATGTCCGAGCGACG                                                            >  SRR4302547.5751136/1‑94 (MQ=60)

                                     AAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCC                                                          <  SRR4302547.6533517/94‑1 (MQ=60)

                                      AATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCA                                                         <  SRR4302547.5863641/94‑1 (MQ=60)

                                       ATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAG                                                        >  SRR4302547.593504/1‑94 (MQ=60)

                                       ATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGGTTTAATCCCTTTATGTCCGAGCGACGCCAG                                                        >  SRR4302547.4885522/1‑94 (MQ=60)

                                        TTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGC                                                       <  SRR4302547.3044840/94‑1 (MQ=60)

                                        TTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGC                                                       <  SRR4302547.4939684/94‑1 (MQ=60)

                                         TCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCG                                                      <  SRR4302547.2781827/94‑1 (MQ=60)

                                             GTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATT                                                  >  SRR4302547.973264/1‑94 (MQ=60)

                                              TTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTT                                                 <  SRR4302547.1828724/94‑1 (MQ=60)

                                              TTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTT                                                 >  SRR4302547.2756781/1‑94 (MQ=60)

                                                TGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAA                                               <  SRR4302547.434486/94‑1 (MQ=60)

                                                 GCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAAC                                              <  SRR4302547.3663181/94‑1 (MQ=60)

                                                  CCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACA                                             >  SRR4302547.1688539/1‑94 (MQ=60)

                                                  CCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACA                                             <  SRR4302547.2182499/94‑1 (MQ=60)

                                                  CCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACA                                             <  SRR4302547.4362951/94‑1 (MQ=60)

                                                   CGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAG                                            <  SRR4302547.4901179/94‑1 (MQ=60)

                                                   CGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAG                                            <  SRR4302547.5575592/94‑1 (MQ=60)

                                                    GACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGC                                           >  SRR4302547.4906936/1‑94 (MQ=60)

                                                     ACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCA                                          >  SRR4302547.789063/1‑94 (MQ=60)

                                                      CCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCAT                                         <  SRR4302547.1999797/94‑1 (MQ=60)

                                                       CAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATG                                        >  SRR4302547.4457689/1‑94 (MQ=60)

                                                       CAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATG                                        <  SRR4302547.4732678/94‑1 (MQ=60)

                                                         GCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAG                                      <  SRR4302547.2525634/94‑1 (MQ=60)

                                                           GCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTG                                    <  SRR4302547.53480/94‑1 (MQ=60)

                                                           GCATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTG                                    >  SRR4302547.124878/1‑94 (MQ=60)

                                                            CATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGG                                   >  SRR4302547.2321161/1‑94 (MQ=60)

                                                            CATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAGTCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGG                                   <  SRR4302547.6700432/94‑1 (MQ=60)

                                                            CATTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGG                                   >  SRR4302547.5630023/1‑94 (MQ=60)

                                                              TTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCT                                 <  SRR4302547.2095581/94‑1 (MQ=60)

                                                              TTGAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCT                                 <  SRR4302547.202655/94‑1 (MQ=60)

                                                                GAATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTA                               >  SRR4302547.4597370/1‑94 (MQ=60)

                                                                 AATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAA                              >  SRR4302547.708482/1‑94 (MQ=60)

                                                                  ATAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAAC                             >  SRR4302547.6076076/1‑94 (MQ=60)

                                                                   TAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACT                            <  SRR4302547.3847202/94‑1 (MQ=60)

                                                                   TAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACT                            <  SRR4302547.6774477/94‑1 (MQ=60)

                                                                   TAACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACT                            <  SRR4302547.1411839/94‑1 (MQ=60)

                                                                    AACATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTC                           >  SRR4302547.5204320/1‑94 (MQ=60)

                                                                     ACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCC                          <  SRR4302547.426306/94‑1 (MQ=60)

                                                                      CATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCC                         >  SRR4302547.3357887/1‑94 (MQ=60)

                                                                      CATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCC                         <  SRR4302547.936359/94‑1 (MQ=60)

                                                                       ATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCT                        <  SRR4302547.1750375/94‑1 (MQ=60)

                                                                       ATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCT                        <  SRR4302547.3259489/94‑1 (MQ=60)

                                                                       ATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCT                        >  SRR4302547.3679925/1‑94 (MQ=60)

                                                                       ATTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCT                        <  SRR4302547.532075/94‑1 (MQ=60)

                                                                        TTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTG                       <  SRR4302547.3113433/94‑1 (MQ=60)

                                                                        TTAACCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTG                       <  SRR4302547.3737542/94‑1 (MQ=60)

                                                                          AACCCGGCCCGCCCGAACGTTGTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGT                     >  SRR4302547.3854975/1‑94 (MQ=60)

                                                                           ACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTA                    >  SRR4302547.517624/1‑94 (MQ=60)

                                                                            CCCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAA                   <  SRR4302547.3700209/94‑1 (MQ=60)

                                                                             CCGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAA                  <  SRR4302547.1691327/94‑1 (MQ=60)

                                                                              CGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAA                 >  SRR4302547.822726/1‑94 (MQ=60)

                                                                              CGGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAA                 >  SRR4302547.4290661/1‑94 (MQ=60)

                                                                               GGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAG                >  SRR4302547.6019027/1‑94 (MQ=60)

                                                                               GGCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAG                >  SRR4302547.4001968/1‑94 (MQ=60)

                                                                                GCCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGA               >  SRR4302547.1774382/1‑94 (MQ=60)

                                                                                 CCCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGAT              <  SRR4302547.4761805/94‑1 (MQ=60)

                                                                                  CCGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATC             <  SRR4302547.6021275/94‑1 (MQ=60)

                                                                                   CGCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCA            >  SRR4302547.3984518/1‑94 (MQ=60)

                                                                                    GCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAG           <  SRR4302547.307807/94‑1 (MQ=60)

                                                                                    GCCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAG           >  SRR4302547.4251571/1‑94 (MQ=60)

                                                                                     CCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGC          >  SRR4302547.369833/1‑94 (MQ=60)

                                                                                     CCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGC          <  SRR4302547.6172101/94‑1 (MQ=60)

                                                                                     CCCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGC          <  SRR4302547.836081/94‑1 (MQ=60)

                                                                                      CCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCG         >  SRR4302547.1409624/1‑94 (MQ=60)

                                                                                      CCGAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCG         <  SRR4302547.2110125/94‑1 (MQ=60)

                                                                                        GAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTG       <  SRR4302547.6044279/94‑1 (MQ=60)

                                                                                        GAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTG       >  SRR4302547.5386810/1‑94 (MQ=60)

                                                                                        GAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTG       >  SRR4302547.2817799/1‑94 (MQ=60)

                                                                                        GAACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTG       >  SRR4302547.952378/1‑94 (MQ=60)

                                                                                         AACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGA      >  SRR4302547.1744223/1‑94 (MQ=60)

                                                                                         AACGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGA      >  SRR4302547.5851390/1‑94 (MQ=60)

                                                                                           CGCTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATA    >  SRR4302547.10169/1‑94 (MQ=60)

                                                                                             CTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAAT  >  SRR4302547.5432637/1‑94 (MQ=60)

TATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAAT  >  NC_000913/3336742‑3336928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`