Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,352,254 2 bp→GC intergenic (‑569/‑12) yhcC ← / → gltB hypothetical protein/glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,2540CG99.6% 898.2 / NA 247intergenic (‑569/‑13)yhcC/gltBhypothetical protein/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (141/105);  minor base A (1/0);  total (142/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,352,2550GC99.6% 897.4 / ‑5.0 247intergenic (‑570/‑12)yhcC/gltBhypothetical protein/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (142/104);  minor base A (0/1);  total (142/105)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 255 total aligned reads displayed.

AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGC  >  NC_000913/3352167‑3352341
                                                                                       ||                                                                                      
agcagcCTGCTCATCATATTTATGCAGTAATTGAGATTCCCTCTTCAGCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGCATGCGAgg                                                                                    >  2:3662077/1‑93 (MQ=255)
   agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc                                                                                 >  1:1053944/1‑93 (MQ=255)
    gcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg                                                                                >  1:380854/1‑93 (MQ=255)
    gcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg                                                                                <  1:1641232/93‑1 (MQ=255)
     ccTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc                                                                               >  1:4815659/1‑93 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt                                                                             >  1:5316660/1‑93 (MQ=255)
       tGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt                                                                             >  1:1857343/1‑93 (MQ=255)
         cTCATCGTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAt                                                                           >  1:5518845/1‑93 (MQ=255)
         cTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAt                                                                           >  2:5705725/1‑93 (MQ=255)
         cTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAt                                                                           >  1:488908/1‑93 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                          <  1:117123/93‑1 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                          <  1:4513822/93‑1 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                          >  2:2789533/1‑93 (MQ=255)
          tcatcaTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                          >  2:725557/1‑93 (MQ=255)
           catcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGa                                                                         <  1:519148/93‑1 (MQ=255)
             tcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                       >  2:6357665/1‑93 (MQ=255)
             tcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                       >  2:1164854/1‑93 (MQ=255)
             tcatATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                       >  1:6289887/1‑93 (MQ=255)
                tatTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc                                                                    >  2:1643016/1‑93 (MQ=255)
                 atTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCa                                                                   <  2:3809400/93‑1 (MQ=255)
                   ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa                                                                 >  1:853366/1‑93 (MQ=255)
                      tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc                                                              <  2:192649/93‑1 (MQ=255)
                      tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc                                                              >  2:5470008/1‑93 (MQ=255)
                         aGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGt                                                           <  2:1493441/93‑1 (MQ=255)
                          gTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTc                                                          <  1:5586476/93‑1 (MQ=255)
                           tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCg                                                         >  1:523681/1‑93 (MQ=255)
                            aaTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                        <  2:5026439/93‑1 (MQ=255)
                            aaTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                        <  2:2673651/93‑1 (MQ=255)
                            aaTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                        <  2:6580938/93‑1 (MQ=255)
                              ttGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCa                                                      >  2:4446518/1‑93 (MQ=255)
                                  gaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGct                                                  >  2:2627293/1‑93 (MQ=255)
                                    tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctct                                                >  1:3451108/1‑93 (MQ=255)
                                    tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCCCCACGctct                                                >  1:2641803/1‑93 (MQ=255)
                                      cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt                                              >  1:125178/1‑93 (MQ=255)
                                      cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt                                              >  1:1740983/1‑93 (MQ=255)
                                      cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGATCttt                                              >  1:1342578/1‑93 (MQ=255)
                                       ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc                                             >  2:2461132/1‑93 (MQ=255)
                                       ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc                                             >  2:4874862/1‑93 (MQ=255)
                                       ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc                                             >  1:3224920/1‑93 (MQ=255)
                                        ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt                                            >  2:2927515/1‑93 (MQ=255)
                                        ctctTCACCGTATTAACCGACGCTAAAAGGACAACAAGGGTGTGAAGGCGAGGCGCTCGTATGACACGCAAACCCCGTCGCCACGCtctctca                                            >  2:6643572/1‑92 (MQ=255)
                                         tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                           >  2:4553278/1‑93 (MQ=255)
                                         tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                           <  1:3027846/93‑1 (MQ=255)
                                         tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                           <  1:5880574/93‑1 (MQ=255)
                                         tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACCCAAACTCCGTCGCCCAGCTCTTTCtg                                           >  1:601272/1‑93 (MQ=255)
                                          ctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                          <  2:4333232/93‑1 (MQ=255)
                                          ctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                          <  1:2421615/93‑1 (MQ=255)
                                          ctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGAGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                          <  2:867678/93‑1 (MQ=255)
                                            tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGc                                        <  2:3887333/93‑1 (MQ=255)
                                             cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc                                       >  1:3889281/1‑93 (MQ=255)
                                              aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc                                      >  1:5079889/1‑93 (MQ=255)
                                              aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc                                      >  1:5775345/1‑93 (MQ=255)
                                               ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg                                     >  2:2585715/1‑93 (MQ=255)
                                               ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg                                     <  2:2212090/93‑1 (MQ=255)
                                               ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg                                     >  1:180356/1‑93 (MQ=255)
                                               ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg                                     >  1:2011181/1‑93 (MQ=255)
                                               ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg                                     <  2:1667585/93‑1 (MQ=255)
                                               ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg                                     >  2:3572334/1‑93 (MQ=255)
                                                cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt                                    >  2:6003728/1‑93 (MQ=255)
                                                cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt                                    <  2:6542384/93‑1 (MQ=255)
                                                cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt                                    <  1:3759343/93‑1 (MQ=255)
                                                 gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg                                   >  1:3349814/1‑93 (MQ=255)
                                                  tATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc                                  >  1:3426586/1‑93 (MQ=255)
                                                   aTTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcg                                 >  2:5131059/1‑93 (MQ=255)
                                                   aTTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcg                                 <  1:6443573/93‑1 (MQ=255)
                                                    ttAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc                                >  2:4970530/1‑93 (MQ=255)
                                                     tAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCa                               <  1:3395351/93‑1 (MQ=255)
                                                     tAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCa                               <  1:1451162/93‑1 (MQ=255)
                                                       aCCGATGCGAAAAGGACAACAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGagcagc                             >  1:4227292/1‑93 (MQ=255)
                                                        ccGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg                            <  2:1821159/93‑1 (MQ=255)
                                                          gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTGTTACTGTGCCCGTGCGCAGCGGt                          >  2:1023767/1‑93 (MQ=255)
                                                          gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt                          >  1:4605706/1‑93 (MQ=255)
                                                           aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt                         <  1:2980508/93‑1 (MQ=255)
                                                             gCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCg                       <  1:2616449/93‑1 (MQ=255)
                                                             gCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCg                       >  1:5823917/1‑93 (MQ=255)
                                                              cGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCgg                      >  2:6430600/1‑93 (MQ=255)
                                                               gAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa                     >  2:3069599/1‑93 (MQ=255)
                                                                aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGaa                    <  2:4243871/93‑1 (MQ=255)
                                                                 aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAg                   >  2:5844306/1‑93 (MQ=255)
                                                                  aaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGt                  >  1:2092502/1‑93 (MQ=255)
                                                                  aaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGt                  <  1:2394328/93‑1 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 >  2:429611/1‑93 (MQ=255)
                                                                   aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                 >  2:2960284/1‑93 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                <  1:2769684/93‑1 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                <  1:5346311/93‑1 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgg                <  2:1727204/93‑1 (MQ=255)
                                                                    ggACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTACGCAGCGGTTCGGAAGTgg                <  1:6481565/93‑1 (MQ=255)
                                                                      acaacaAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTgggg              >  2:3963206/1‑93 (MQ=255)
                                                                       caacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACTCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGt             <  2:3297432/93‑1 (MQ=255)
                                                                       caacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGt             <  2:6214174/93‑1 (MQ=255)
                                                                        aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGtt            <  1:1599047/93‑1 (MQ=255)
                                                                         acaaGGGGTCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc           <  1:1127210/93‑1 (MQ=255)
                                                                         acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTc           >  1:2833594/1‑93 (MQ=255)
                                                                           aaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCACGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTccc         >  2:4792710/1‑93 (MQ=255)
                                                                            aGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCg        >  2:4688838/1‑93 (MQ=255)
                                                                             gggggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGc       >  1:6156968/1‑93 (MQ=255)
                                                                              ggggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa      <  1:920153/93‑1 (MQ=255)
                                                                              ggggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTAGCCACGCTCTTTCTGTGACCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCa      >  1:4345968/1‑93 (MQ=255)
                                                                                ggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCaga    <  1:3164477/93‑1 (MQ=255)
                                                                                  cGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGc  <  1:3064099/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGC  >  NC_000913/3352167‑3352341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  NC_000913/3352161‑3352343
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TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATG                                                                                           >  SRR4302547.6260714/1‑94 (MQ=60)
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATG                                                                                           <  SRR4302547.5674228/94‑1 (MQ=60)
        CAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGC                                                                                   >  SRR4302547.1074886/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCG                                                                                  >  SRR4302547.387915/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCG                                                                                  >  SRR4302547.5480387/1‑94 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCG                                                                                  <  SRR4302547.3918493/94‑1 (MQ=60)
         AGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCG                                                                                  <  SRR4302547.1743932/94‑1 (MQ=60)
          GCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGC                                                                                 >  SRR4302547.4915459/1‑94 (MQ=60)
          GCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGC                                                                                 <  SRR4302547.1673671/94‑1 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGT                                                                               <  SRR4302547.5307301/94‑1 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGT                                                                               >  SRR4302547.1894033/1‑94 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGT                                                                               >  SRR4302547.4237118/1‑94 (MQ=60)
            CTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGT                                                                               >  SRR4302547.5426298/1‑94 (MQ=60)
              GCTCATCGTATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAT                                                                             >  SRR4302547.5632770/1‑94 (MQ=60)
              GCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAT                                                                             <  SRR4302547.4668501/94‑1 (MQ=60)
              GCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAT                                                                             >  SRR4302547.497849/1‑94 (MQ=60)
               CTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATG                                                                            >  SRR4302547.2486315/1‑94 (MQ=60)
                TCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGA                                                                           <  SRR4302547.4607080/94‑1 (MQ=60)
                TCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGA                                                                           <  SRR4302547.119248/94‑1 (MQ=60)
                 CATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGAC                                                                          <  SRR4302547.528559/94‑1 (MQ=60)
                  ATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACA                                                                         >  SRR4302547.6420591/1‑94 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGC                                                                      >  SRR4302547.6190671/1‑94 (MQ=60)
                     ATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGC                                                                      >  SRR4302547.3401305/1‑94 (MQ=60)
                       ATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAA                                                                    <  SRR4302547.3888764/94‑1 (MQ=60)
                        TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAA                                                                   >  SRR4302547.869663/1‑94 (MQ=60)
                         TTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAC                                                                  >  SRR4302547.3186272/1‑94 (MQ=60)
                            TGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCC                                                               <  SRR4302547.922794/94‑1 (MQ=60)
                            TGCAGTAATTGAGACCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCC                                                               <  SRR4302547.5704472/94‑1 (MQ=60)
                             GCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCG                                                              >  SRR4302547.3493901/1‑94 (MQ=60)
                               AGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTC                                                            >  SRR4302547.6109657/1‑94 (MQ=60)
                                GTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCG                                                           >  SRR4302547.533160/1‑94 (MQ=60)
                                GTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCG                                                           <  SRR4302547.5701705/94‑1 (MQ=60)
                                 TAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGC                                                          <  SRR4302547.5251934/94‑1 (MQ=60)
                                     TGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGGCACGGAAACCCCGTCGCCACG                                                      >  SRR4302547.6593264/1‑94 (MQ=60)
                                     TGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACG                                                      <  SRR4302547.186884/94‑1 (MQ=60)
                                     TGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACG                                                      >  SRR4302547.1403440/1‑94 (MQ=60)
                                     TGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACG                                                      <  SRR4302547.6267761/94‑1 (MQ=60)
                                     TGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCAACG                                                      >  SRR4302547.715539/1‑94 (MQ=60)
                                      GAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGC                                                     >  SRR4302547.3190590/1‑94 (MQ=60)
                                      GAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGC                                                     >  SRR4302547.3105316/1‑94 (MQ=60)
                                       AGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCT                                                    <  SRR4302547.5052514/94‑1 (MQ=60)
                                       AGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCT                                                    >  SRR4302547.1336885/1‑94 (MQ=60)
                                       AGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCT                                                    <  SRR4302547.895251/94‑1 (MQ=60)
                                        GATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTC                                                   >  SRR4302547.5942944/1‑94 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCT                                                  <  SRR4302547.6432223/94‑1 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCT                                                  >  SRR4302547.3521458/1‑94 (MQ=60)
                                         ATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCCCCACGCTCT                                                  >  SRR4302547.2694970/1‑94 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 <  SRR4302547.245742/94‑1 (MQ=60)
                                          TCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTT                                                 <  SRR4302547.3066488/94‑1 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                >  SRR4302547.2575369/1‑94 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                >  SRR4302547.1775234/1‑94 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTT                                                >  SRR4302547.127452/1‑94 (MQ=60)
                                           CCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGATCTTT                                                >  SRR4302547.1369545/1‑94 (MQ=60)
                                            CCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTC                                               >  SRR4302547.3289274/1‑94 (MQ=60)
                                            CCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTC                                               <  SRR4302547.4798949/94‑1 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTG                                             >  SRR4302547.4580370/1‑94 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTG                                             >  SRR4302547.5994203/1‑94 (MQ=60)
                                              CTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACCCAAACTCCGTCGCCCAGCTCTTTCTG                                             >  SRR4302547.612399/1‑94 (MQ=60)
                                               TCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGT                                            <  SRR4302547.3088053/94‑1 (MQ=60)
                                               TCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGT                                            >  SRR4302547.2952394/1‑94 (MQ=60)
                                               TCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGT                                            <  SRR4302547.6002332/94‑1 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           <  SRR4302547.2792241/94‑1 (MQ=60)
                                                CTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTG                                           <  SRR4302547.2470633/94‑1 (MQ=60)
                                                 TTCACCGTATTAACCGATGCGAAAAGTACAACAAGGGGGCGGATGCGAGGCCAGCGTATGACACGCAAACCCCGCCGCCAAGCTCTTTCTaggc                                          >  SRR4302547.1888089/1‑90 (MQ=57)
                                                  TCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCC                                         >  SRR4302547.3968389/1‑94 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        >  SRR4302547.5894799/1‑94 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        <  SRR4302547.1520703/94‑1 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        >  SRR4302547.5184700/1‑94 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCC                                        >  SRR4302547.2319344/1‑94 (MQ=60)
                                                   CACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCACGTCGCCACGCTCTTTCTGTGCCC                                        >  SRR4302547.3350084/1‑94 (MQ=60)
                                                    ACCGTATTACCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCG                                       >  SRR4302547.6613647/1‑94 (MQ=60)
                                                    ACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCG                                       >  SRR4302547.183740/1‑94 (MQ=60)
                                                    ACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCG                                       >  SRR4302547.2051197/1‑94 (MQ=60)
                                                      CGTATTAACTGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTG                                     >  SRR4302547.6577758/1‑94 (MQ=60)
                                                      CGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTG                                     >  SRR4302547.3417740/1‑94 (MQ=60)
                                                      CGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTG                                     <  SRR4302547.3836045/94‑1 (MQ=60)
                                                       GTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGC                                    >  SRR4302547.3496378/1‑94 (MQ=60)
                                                       GTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGC                                    >  SRR4302547.3863570/1‑94 (MQ=60)
                                                       GTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGC                                    >  SRR4302547.5008080/1‑94 (MQ=60)
                                                         ATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGC                                  >  SRR4302547.3271638/1‑94 (MQ=60)
                                                         ATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGC                                  <  SRR4302547.6577847/94‑1 (MQ=60)
                                                          TTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCA                                 >  SRR4302547.3490529/1‑94 (MQ=60)
                                                          TTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCA                                 <  SRR4302547.1177024/94‑1 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                <  SRR4302547.3464439/94‑1 (MQ=60)
                                                           TAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAG                                <  SRR4302547.1480183/94‑1 (MQ=60)
                                                            AACCGATGCGAAAAGGACAACAGGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGAGCAGC                               >  SRR4302547.4313693/1‑94 (MQ=60)
                                                            AACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGC                               >  SRR4302547.159647/1‑94 (MQ=60)
                                                             ACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCG                              >  SRR4302547.2979016/1‑94 (MQ=60)
                                                             ACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCG                              >  SRR4302547.16409/1‑94 (MQ=60)
                                                             ACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCG                              >  SRR4302547.2683879/1‑94 (MQ=60)
                                                              CCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGG                             >  SRR4302547.4980965/1‑94 (MQ=60)
                                                               CGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGT                            >  SRR4302547.4701017/1‑94 (MQ=60)
                                                                GATGCGAAAAGGACAACGAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           <  SRR4302547.1153121/94‑1 (MQ=60)
                                                                GATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           <  SRR4302547.2719486/94‑1 (MQ=60)
                                                                GATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           <  SRR4302547.4768994/94‑1 (MQ=60)
                                                                GATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTT                           <  SRR4302547.1779219/94‑1 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          <  SRR4302547.5331062/94‑1 (MQ=60)
                                                                 ATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTC                          <  SRR4302547.3039814/94‑1 (MQ=60)
                                                                  TGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCG                         >  SRR4302547.5944436/1‑94 (MQ=60)
                                                                  TGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCG                         <  SRR4302547.2086100/94‑1 (MQ=60)
                                                                   GCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGG                        <  SRR4302547.2669165/94‑1 (MQ=60)
                                                                    CGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACACTCTCTCTGTGCCCGTGCGCAGCGGTTCGGA                       >  SRR4302547.1146447/1‑94 (MQ=60)
                                                                     GAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAA                      >  SRR4302547.1974221/1‑94 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     >  SRR4302547.1302953/1‑94 (MQ=60)
                                                                      AAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAG                     >  SRR4302547.5759949/1‑94 (MQ=60)
                                                                       AAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGT                    >  SRR4302547.2134206/1‑94 (MQ=60)
                                                                       AAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGT                    <  SRR4302547.5366734/94‑1 (MQ=60)
                                                                       AAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGT                    <  SRR4302547.3689559/94‑1 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   >  SRR4302547.3455756/1‑94 (MQ=60)
                                                                        AAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG                   <  SRR4302547.2442826/94‑1 (MQ=60)
                                                                         AGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGG                  >  SRR4302547.1214854/1‑94 (MQ=60)
                                                                          GGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGG                 <  SRR4302547.2825175/94‑1 (MQ=60)
                                                                          GGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGG                 >  SRR4302547.2878357/1‑94 (MQ=60)
                                                                          GGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGG                 <  SRR4302547.5456532/94‑1 (MQ=60)
                                                                          GGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTACGCAGCGGTTCGGAAGTGGG                 <  SRR4302547.6616507/94‑1 (MQ=60)
                                                                            ACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGT               <  SRR4302547.4442625/94‑1 (MQ=60)
                                                                             CAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              <  SRR4302547.56794/94‑1 (MQ=60)
                                                                             CAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              >  SRR4302547.5999710/1‑94 (MQ=60)
                                                                             CAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTT              <  SRR4302547.2024883/94‑1 (MQ=60)
                                                                             CAACAAGGGGGCGAATACGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGCGGGGAT              >  SRR4302547.1844689/1‑94 (MQ=60)
                                                                              AACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             >  SRR4302547.2890252/1‑94 (MQ=60)
                                                                              AACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             <  SRR4302547.1630777/94‑1 (MQ=60)
                                                                              AACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             >  SRR4302547.5427729/1‑94 (MQ=60)
                                                                              AACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             <  SRR4302547.5419589/94‑1 (MQ=60)
                                                                              AACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             >  SRR4302547.4085375/1‑94 (MQ=60)
                                                                              AACAAGGGGGCGAATGCGAGCCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTC             <  SRR4302547.6372350/94‑1 (MQ=60)
                                                                               ACAAGGGGTCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCC            <  SRR4302547.1149956/94‑1 (MQ=60)
                                                                                CAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCC           <  SRR4302547.5562615/94‑1 (MQ=60)
                                                                                CAAGGGGGCGAATGCGAGGCGCGCGTATGACACCCAAGCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGCAGTGCGGTTCCC           <  SRR4302547.2083741/94‑1 (MQ=60)
                                                                                 AAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          <  SRR4302547.3521324/94‑1 (MQ=60)
                                                                                 AAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCG          >  SRR4302547.3682701/1‑94 (MQ=60)
                                                                                  AGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGGCCGTGCGCAGCGGATCGGAAGGGGGGTTCCCGC         >  SRR4302547.3518247/1‑94 (MQ=60)
                                                                                  AGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGC         >  SRR4302547.6284741/1‑94 (MQ=60)
                                                                                   GGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTAGCCACGCTCTTTCTGTGACCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCA        >  SRR4302547.4435302/1‑94 (MQ=60)
                                                                                    GGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAG       <  SRR4302547.937920/94‑1 (MQ=60)
                                                                                    GGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAG       <  SRR4302547.1709036/94‑1 (MQ=60)
                                                                                     GGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGA      <  SRR4302547.2168819/94‑1 (MQ=60)
                                                                                      GGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAG     <  SRR4302547.3227436/94‑1 (MQ=60)
                                                                                      GGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAG     <  SRR4302547.3061860/94‑1 (MQ=60)
                                                                                      GGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCATGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAG     <  SRR4302547.438685/94‑1 (MQ=60)
                                                                                       GCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGC    <  SRR4302547.985322/94‑1 (MQ=60)
                                                                                        CGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCC   <  SRR4302547.3125025/94‑1 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302547.6037660/94‑1 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302547.1468590/94‑1 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  SRR4302547.1426001/1‑94 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  SRR4302547.1332864/1‑94 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  SRR4302547.1164059/1‑94 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302547.4706543/94‑1 (MQ=60)
                                                                                         GAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  <  SRR4302547.6216682/94‑1 (MQ=60)
                                                                                             |                                                                                         
TGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCT  >  NC_000913/3352161‑3352343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑