Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,452,089 T→C Q316R (CAG→CGG)  yheD ← YheD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,452,0890TC82.4% 616.0 / 126.0 262Q316R (CAG→CGG) yheDYheD
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base C (139/77);  total (164/98)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 266 total aligned reads displayed.

TTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAG  >  NC_000913/3451999‑3452179
                                                                                          |                                                                                          
ttAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGa                                                                                          >  2:5207100/1‑93 (MQ=255)
   aGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCg                                                                                       <  1:5288908/93‑1 (MQ=255)
   aGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCg                                                                                       >  1:5510530/1‑93 (MQ=255)
   aGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCg                                                                                       <  2:1454504/93‑1 (MQ=255)
   aGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCg                                                                                       <  2:2710009/93‑1 (MQ=255)
    gcgatgcgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGc                                                                                      <  2:6398841/93‑1 (MQ=255)
      gatgcgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCAc                                                                                    >  2:1984956/1‑93 (MQ=255)
       atgcgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCAcc                                                                                   >  1:1935884/1‑93 (MQ=255)
       atgcgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCAcc                                                                                   <  2:6207308/93‑1 (MQ=255)
       atgcgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCAcc                                                                                   <  1:2663791/93‑1 (MQ=255)
        tgcgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCa                                                                                  <  2:4881817/93‑1 (MQ=255)
         gcgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAg                                                                                 <  2:6045428/93‑1 (MQ=255)
          cgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAgg                                                                                >  2:2346907/1‑93 (MQ=255)
          cgatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAgg                                                                                >  2:5822732/1‑93 (MQ=255)
           gatCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGc                                                                               >  1:1472545/1‑93 (MQ=255)
             tCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCg                                                                             >  1:3452474/1‑93 (MQ=255)
               cAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGaa                                                                           >  1:4898033/1‑93 (MQ=255)
               cAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGaa                                                                           >  2:3986136/1‑93 (MQ=255)
               cAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGaa                                                                           >  1:5902590/1‑93 (MQ=255)
               cAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGaa                                                                           >  2:5309718/1‑93 (MQ=255)
                aGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAAt                                                                          <  1:5409510/93‑1 (MQ=255)
                 gggCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATc                                                                         >  1:2596922/1‑93 (MQ=255)
                 gggCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATc                                                                         <  2:6495287/93‑1 (MQ=255)
                  ggCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCa                                                                        <  1:423641/93‑1 (MQ=255)
                    cAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGc                                                                      >  2:54196/1‑93 (MQ=255)
                     aaaTTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCt                                                                     >  2:3081514/1‑93 (MQ=255)
                     aaaTTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCt                                                                     >  2:2616381/1‑93 (MQ=255)
                      aaTTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTg                                                                    <  2:2280096/93‑1 (MQ=255)
                      aaTTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTg                                                                    >  1:4993147/1‑93 (MQ=255)
                       aTTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTgg                                                                   <  1:1961312/93‑1 (MQ=255)
                        ttCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTggg                                                                  >  1:1256646/1‑93 (MQ=255)
                        ttCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTggg                                                                  >  1:788801/1‑93 (MQ=255)
                           tGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGAcc                                                               <  2:3495451/93‑1 (MQ=255)
                            gATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCt                                                              >  2:6545420/1‑93 (MQ=255)
                             aTCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTc                                                             >  2:4407462/1‑93 (MQ=255)
                              tCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCg                                                            >  1:1797373/1‑93 (MQ=255)
                              tCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCg                                                            >  2:1208494/1‑93 (MQ=255)
                              tCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCg                                                            <  1:2309907/93‑1 (MQ=255)
                               cTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGt                                                           >  2:3338241/1‑93 (MQ=255)
                                tACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTa                                                          >  2:4277743/1‑93 (MQ=255)
                                tACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTa                                                          >  2:2493677/1‑93 (MQ=255)
                                  ccAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTAcc                                                        >  1:6000522/1‑93 (MQ=255)
                                   cAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTAccc                                                       >  2:6348423/1‑93 (MQ=255)
                                    aaTGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCCTAcccc                                                      >  1:3782615/1‑93 (MQ=255)
                                     aTGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTAccccc                                                     <  1:324319/93‑1 (MQ=255)
                                     aTGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTAccccc                                                     <  1:74021/93‑1 (MQ=255)
                                      tGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCa                                                    <  2:4707228/93‑1 (MQ=255)
                                      tGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCa                                                    <  1:2965165/93‑1 (MQ=255)
                                      tGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCa                                                    >  2:1390068/1‑93 (MQ=255)
                                       gTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCAt                                                   <  1:3319000/93‑1 (MQ=255)
                                        tttGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATa                                                  >  1:351417/1‑93 (MQ=255)
                                              aagaagCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGt                                            >  1:44922/1‑93 (MQ=255)
                                                gaagCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGtat                                          <  1:6376364/93‑1 (MQ=255)
                                                 aagCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGACCGAATCAGCTGTGACCTCGTACCCCCATACGCCGcata                                         >  1:1184506/1‑93 (MQ=255)
                                                  agCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATaa                                        >  1:1045770/1‑93 (MQ=255)
                                                  agCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGACGTATaa                                        >  1:2202540/1‑93 (MQ=255)
                                                   gCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAac                                       >  1:2263711/1‑93 (MQ=255)
                                                    cATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAaca                                      <  2:426463/93‑1 (MQ=255)
                                                     aTTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAacac                                     <  1:2874753/93‑1 (MQ=255)
                                                      ttCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACg                                    >  2:5023979/1‑93 (MQ=255)
                                                      ttCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTTGGACCTCGTACCCCCATACGCCGTATAACACg                                    >  2:477754/1‑93 (MQ=255)
                                                      ttCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACg                                    >  2:5686928/1‑93 (MQ=255)
                                                       tCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGc                                   >  2:3138826/1‑93 (MQ=255)
                                                         ccACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCtt                                 >  1:272911/1‑93 (MQ=255)
                                                         ccACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCtt                                 >  2:897571/1‑93 (MQ=255)
                                                          cACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTa                                <  2:2813391/93‑1 (MQ=255)
                                                           aCTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAg                               >  1:554507/1‑93 (MQ=255)
                                                           aCTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAg                               >  2:3599737/1‑93 (MQ=255)
                                                             ttttACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAgcg                             >  1:2710998/1‑93 (MQ=255)
                                                               ttACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGcc                           <  2:3568114/93‑1 (MQ=255)
                                                               ttACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGcc                           <  1:567930/93‑1 (MQ=255)
                                                                 aCATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTc                         <  2:4171884/93‑1 (MQ=255)
                                                                 aCATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTc                         >  1:1414046/1‑93 (MQ=255)
                                                                 aCATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTc                         >  2:1846054/1‑93 (MQ=255)
                                                                  cATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCa                        >  2:4450022/1‑93 (MQ=255)
                                                                   aTGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTCGCGCCCCAc                       >  2:1626848/1‑93 (MQ=255)
                                                                     gCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTa                     >  2:2554843/1‑93 (MQ=255)
                                                                     gCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTa                     <  1:4822408/93‑1 (MQ=255)
                                                                        aGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATcg                  >  1:577485/1‑93 (MQ=255)
                                                                         gCCCCGCTGTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATcgc                 >  1:6499909/1‑93 (MQ=255)
                                                                          ccccGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATcgct                >  1:4280620/1‑92 (MQ=255)
                                                                           cccGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCTTATAACACGCTTAGCGCCTCACTATCgcgc               >  2:4072983/1‑93 (MQ=255)
                                                                           cccGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCgcgc               <  2:4021436/93‑1 (MQ=255)
                                                                           cccGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCgcgc               >  1:2003336/1‑93 (MQ=255)
                                                                            ccGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCa              <  2:3969861/93‑1 (MQ=255)
                                                                            ccGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCa              >  2:379565/1‑93 (MQ=255)
                                                                            ccGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCa              >  1:1490859/1‑93 (MQ=255)
                                                                             cGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCAt             >  1:363245/1‑93 (MQ=255)
                                                                              gCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATc            >  1:3059425/1‑93 (MQ=255)
                                                                              gCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATc            <  2:4496220/93‑1 (MQ=255)
                                                                               ctctTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATca           >  2:2981590/1‑93 (MQ=255)
                                                                               ctctTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATca           >  2:6111162/1‑93 (MQ=255)
                                                                                 ctTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATcacc         >  1:65809/1‑93 (MQ=255)
                                                                                  ttACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATcacca        <  1:907945/93‑1 (MQ=255)
                                                                                   tACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATcaccac       >  2:5902731/1‑93 (MQ=255)
                                                                                     cTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACAt     >  2:769932/1‑93 (MQ=255)
                                                                                      tGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATg    <  1:3812305/93‑1 (MQ=255)
                                                                                      tGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATg    <  2:2680941/93‑1 (MQ=255)
                                                                                      tGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATg    >  1:467288/1‑93 (MQ=255)
                                                                                       gCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGa   <  1:1338366/93‑1 (MQ=255)
                                                                                          |                                                                                          
TTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAG  >  NC_000913/3451999‑3452179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAGC  >  NC_000913/3451996‑3452180
                                                                                             |                                                                                           
ACTTTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCC                                                                                             >  SRR4302547.2191916/1‑94 (MQ=60)
ACTTTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCC                                                                                             >  SRR4302547.6755822/1‑94 (MQ=60)
ACTTTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCC                                                                                             <  SRR4302547.6341357/94‑1 (MQ=60)
 CTTTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCG                                                                                            >  SRR4302547.5508646/1‑94 (MQ=60)
  TTTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCTTTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGA                                                                                           <  SRR4302547.586116/94‑1 (MQ=60)
   TTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGAT                                                                                          >  SRR4302547.3549334/1‑94 (MQ=60)
    TAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATC                                                                                         <  SRR4302547.4751590/94‑1 (MQ=60)
     AAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCG                                                                                        >  SRR4302547.1802123/1‑94 (MQ=60)
     AAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCG                                                                                        >  SRR4302547.5624299/1‑94 (MQ=60)
      AGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGC                                                                                       >  SRR4302547.5026270/1‑94 (MQ=60)
      AGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGC                                                                                       <  SRR4302547.5398017/94‑1 (MQ=60)
      AGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTACCCGATCGC                                                                                       >  SRR4302547.5026269/1‑94 (MQ=60)
         GATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACC                                                                                    >  SRR4302547.1974277/1‑94 (MQ=60)
          ATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCA                                                                                   <  SRR4302547.2717318/94‑1 (MQ=60)
          ATGCGACCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCA                                                                                   <  SRR4302547.4878794/94‑1 (MQ=60)
           TGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAG                                                                                  <  SRR4302547.2720179/94‑1 (MQ=60)
             CGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGC                                                                                >  SRR4302547.1501955/1‑94 (MQ=60)
              GATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCC                                                                               >  SRR4302547.6201903/1‑94 (MQ=60)
              GATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCC                                                                               >  SRR4302547.1437922/1‑94 (MQ=60)
              GATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCC                                                                               >  SRR4302547.89782/1‑94 (MQ=60)
               ATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCG                                                                              >  SRR4302547.3522862/1‑94 (MQ=60)
                 CCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAA                                                                            >  SRR4302547.4999457/1‑94 (MQ=60)
                 CCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAA                                                                            >  SRR4302547.6024825/1‑94 (MQ=60)
                 CCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAA                                                                            >  SRR4302547.2091700/1‑94 (MQ=60)
                  CAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAAT                                                                           >  SRR4302547.218758/1‑94 (MQ=60)
                   AGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATC                                                                          >  SRR4302547.2649281/1‑94 (MQ=60)
                   AGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATC                                                                          >  SRR4302547.5558222/1‑94 (MQ=60)
                   AGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATC                                                                          <  SRR4302547.5521361/94‑1 (MQ=60)
                   AGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATC                                                                          >  SRR4302547.4347335/1‑94 (MQ=60)
                     GGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAG                                                                        <  SRR4302547.4634206/94‑1 (MQ=60)
                     GGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAG                                                                        <  SRR4302547.431495/94‑1 (MQ=60)
                     GGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAG                                                                        >  SRR4302547.120342/1‑94 (MQ=60)
                      GCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGC                                                                       <  SRR4302547.3310051/94‑1 (MQ=60)
                      GCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGC                                                                       <  SRR4302547.1896593/94‑1 (MQ=60)
                       CAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCT                                                                      <  SRR4302547.3328106/94‑1 (MQ=60)
                        AAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTG                                                                     <  SRR4302547.5381846/94‑1 (MQ=60)
                        AAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTG                                                                     >  SRR4302547.5096370/1‑94 (MQ=60)
                         AATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGG                                                                    <  SRR4302547.788147/94‑1 (MQ=60)
                          ATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGG                                                                   >  SRR4302547.1281985/1‑94 (MQ=60)
                          ATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGG                                                                   <  SRR4302547.6090063/94‑1 (MQ=60)
                          ATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGG                                                                   >  SRR4302547.803733/1‑94 (MQ=60)
                          ATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGG                                                                   <  SRR4302547.2000252/94‑1 (MQ=60)
                           TTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGA                                                                  >  SRR4302547.2765957/1‑94 (MQ=60)
                               GATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTC                                                              >  SRR4302547.6728461/1‑94 (MQ=60)
                               GATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTC                                                              >  SRR4302547.2863862/1‑94 (MQ=60)
                                ATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCG                                                             >  SRR4302547.1869615/1‑94 (MQ=60)
                                ATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCG                                                             >  SRR4302547.1832928/1‑94 (MQ=60)
                                 TCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGT                                                            <  SRR4302547.2356745/94‑1 (MQ=60)
                                 TCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGT                                                            >  SRR4302547.5794801/1‑94 (MQ=60)
                                    ACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACC                                                         >  SRR4302547.6125042/1‑94 (MQ=60)
                                     CCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCC                                                        >  SRR4302547.3629605/1‑94 (MQ=60)
                                     CCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCC                                                        <  SRR4302547.3017666/94‑1 (MQ=60)
                                      CAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACACC                                                       >  SRR4302547.1261352/1‑94 (MQ=60)
                                      CAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCCTACCCC                                                       >  SRR4302547.3859746/1‑94 (MQ=60)
                                       AATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCC                                                      <  SRR4302547.5797830/94‑1 (MQ=60)
                                        ATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCA                                                     <  SRR4302547.6376633/94‑1 (MQ=60)
                                        ATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCA                                                     <  SRR4302547.330356/94‑1 (MQ=60)
                                        ATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCA                                                     <  SRR4302547.75411/94‑1 (MQ=60)
                                         TGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCAT                                                    <  SRR4302547.3024182/94‑1 (MQ=60)
                                          GTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATA                                                   >  SRR4302547.357945/1‑94 (MQ=60)
                                          GTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATA                                                   <  SRR4302547.3385996/94‑1 (MQ=60)
                                          GTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATA                                                   >  SRR4302547.1982777/1‑94 (MQ=60)
                                            TTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACG                                                 <  SRR4302547.2396298/94‑1 (MQ=60)
                                              GTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCC                                               >  SRR4302547.3267723/1‑94 (MQ=60)
                                                AAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGT                                             >  SRR4302547.45751/1‑94 (MQ=60)
                                                   GAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATA                                          >  SRR4302547.4990892/1‑94 (MQ=60)
                                                   GAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATA                                          <  SRR4302547.6509416/94‑1 (MQ=60)
                                                   GAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGACCGAATCAGCTGTGACCTCGTACCCCCATACGCCGCATA                                          >  SRR4302547.1208434/1‑94 (MQ=60)
                                                   AAAGCATTCCCACTTTTCCATGCCAGCCCCTCATTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATA                                          <  SRR4302547.1696613/94‑1 (MQ=60)
                                                    AAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAA                                         >  SRR4302547.1066437/1‑94 (MQ=60)
                                                    AAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAA                                         >  SRR4302547.1318938/1‑94 (MQ=60)
                                                    AAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGACGTATAA                                         >  SRR4302547.2246787/1‑94 (MQ=60)
                                                     AGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAAC                                        >  SRR4302547.2343613/1‑94 (MQ=60)
                                                     AGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAAC                                        >  SRR4302547.2309463/1‑94 (MQ=60)
                                                     AGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAAC                                        >  SRR4302547.1704288/1‑94 (MQ=60)
                                                       CATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACAC                                      >  SRR4302547.1502676/1‑94 (MQ=60)
                                                        ATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACG                                     <  SRR4302547.2932144/94‑1 (MQ=60)
                                                        ATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACG                                     >  SRR4302547.23336/1‑94 (MQ=60)
                                                        ATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCTTACCCCCATACGCCGTATAACACG                                     >  SRR4302547.2013719/1‑94 (MQ=60)
                                                        ATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACG                                     >  SRR4302547.2777786/1‑94 (MQ=60)
                                                        ATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACG                                     >  SRR4302547.3522565/1‑94 (MQ=60)
                                                         TTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGC                                    >  SRR4302547.6562984/1‑94 (MQ=60)
                                                           CCCACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTT                                  >  SRR4302547.278012/1‑94 (MQ=60)
                                                             CACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAG                                >  SRR4302547.564489/1‑94 (MQ=60)
                                                             CACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAG                                >  SRR4302547.3048213/1‑94 (MQ=60)
                                                              CCTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGC                               <  SRR4302547.2744486/94‑1 (MQ=60)
                                                              ACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGC                               >  SRR4302547.2299265/1‑94 (MQ=60)
                                                              ACTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGC                               >  SRR4302547.4543579/1‑94 (MQ=60)
                                                               CTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCG                              >  SRR4302547.3011974/1‑94 (MQ=60)
                                                               CTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCG                              >  SRR4302547.2765420/1‑94 (MQ=60)
                                                               CTTTTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCG                              <  SRR4302547.6600952/94‑1 (MQ=60)
                                                                  TTACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCT                           <  SRR4302547.578132/94‑1 (MQ=60)
                                                                   TACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTC                          <  SRR4302547.3586032/94‑1 (MQ=60)
                                                                   TACATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTC                          >  SRR4302547.1442326/1‑94 (MQ=60)
                                                                    ACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCA                         <  SRR4302547.3776497/94‑1 (MQ=60)
                                                                     CATGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCAC                        >  SRR4302547.3069989/1‑94 (MQ=60)
                                                                     CAGGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCAC                        <  SRR4302547.4029488/94‑1 (MQ=60)
                                                                      AGGCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACT                       <  SRR4302547.5504846/94‑1 (MQ=60)
                                                                        GCCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTAT                     <  SRR4302547.4922350/94‑1 (MQ=60)
                                                                         CCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATC                    <  SRR4302547.1488149/94‑1 (MQ=60)
                                                                         CCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATC                    <  SRR4302547.1418782/94‑1 (MQ=60)
                                                                         CCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATC                    >  SRR4302547.2695527/1‑94 (MQ=60)
                                                                         CCAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATC                    >  SRR4302547.3879862/1‑94 (MQ=60)
                                                                          CAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCG                   >  SRR4302547.800567/1‑94 (MQ=60)
                                                                          CAGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCG                   >  SRR4302547.587836/1‑94 (MQ=60)
                                                                           AGCCCCGCTGTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGC                  >  SRR4302547.6635194/1‑94 (MQ=60)
                                                                           AGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGC                  >  SRR4302547.747744/1‑94 (MQ=60)
                                                                           AGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGC                  <  SRR4302547.6657803/94‑1 (MQ=60)
                                                                           AGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGC                  >  SRR4302547.579319/1‑94 (MQ=60)
                                                                           AGCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATAGC                  >  SRR4302547.4889520/1‑94 (MQ=60)
                                                                            GCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCT                 >  SRR4302547.4368544/1‑94 (MQ=60)
                                                                            GCCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCG                 <  SRR4302547.5628568/94‑1 (MQ=60)
                                                                             CCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGC                >  SRR4302547.5191566/1‑94 (MQ=60)
                                                                             CCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGC                >  SRR4302547.2043200/1‑94 (MQ=60)
                                                                             CCCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGC                >  SRR4302547.4353591/1‑94 (MQ=60)
                                                                              CCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCA               >  SRR4302547.1520598/1‑94 (MQ=60)
                                                                              CCCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCA               >  SRR4302547.6003031/1‑94 (MQ=60)
                                                                              CCCGCTCTTACTGCCCGAACGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACCCGCTTAGCGCCTCACTATCGCGCA               <  SRR4302547.2628344/94‑1 (MQ=60)
                                                                               CCGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCAT              >  SRR4302547.369998/1‑94 (MQ=60)
                                                                                CGCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATC             >  SRR4302547.3120286/1‑94 (MQ=60)
                                                                                 GCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCA            >  SRR4302547.4042143/1‑94 (MQ=60)
                                                                                 GCTCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCA            >  SRR4302547.1465120/1‑94 (MQ=60)
                                                                                   TCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACC          <  SRR4302547.1213244/94‑1 (MQ=60)
                                                                                   TCTTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACC          >  SRR4302547.67038/1‑94 (MQ=60)
                                                                                     TTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCAC        >  SRR4302547.5602358/1‑94 (MQ=60)
                                                                                     TTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCAC        >  SRR4302547.3512487/1‑94 (MQ=60)
                                                                                     TTACTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCAC        <  SRR4302547.925447/94‑1 (MQ=60)
                                                                                        CTGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATG     >  SRR4302547.475897/1‑94 (MQ=60)
                                                                                         TGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGA    <  SRR4302547.3889995/94‑1 (MQ=60)
                                                                                         TGCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGA    >  SRR4302547.5951535/1‑94 (MQ=60)
                                                                                          GCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAG   <  SRR4302547.20371/94‑1 (MQ=60)
                                                                                          GCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAG   <  SRR4302547.1365266/94‑1 (MQ=60)
                                                                                          GCCCGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAG   <  SRR4302547.3718422/94‑1 (MQ=60)
                                                                                           CCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAGC  <  SRR4302547.5636008/94‑1 (MQ=60)
                                                                                             |                                                                                           
ACTTTAAGCGATGCGATCCAGGGCAAATTCTGATCTACCAATGTTTGTAAAGAAGCATTCCCACTTTTACATGCCAGCCCCGCTCTTACTGCCTGATCGCACCAGGCCGAATCAGCTGGGACCTCGTACCCCCATACGCCGTATAACACGCTTAGCGCCTCACTATCGCGCATCACCACATGAGC  >  NC_000913/3451996‑3452180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑