Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,453,670 T→C T152T (ACT→ACC)  yheE → YheE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,453,6700TC99.0% 740.2 / NA 208T152T (ACT→ACC) yheEYheE
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base C (117/87);  minor base A (0/2);  total (118/90)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

AGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATAC  >  NC_000913/3453580‑3453758
                                                                                          |                                                                                        
aGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAAccct                                                                                        >  1:1097464/1‑93 (MQ=255)
    cTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtccat                                                                                    <  1:5955892/93‑1 (MQ=255)
     ttGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtccatc                                                                                   >  1:3350500/1‑93 (MQ=255)
     ttGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtccatc                                                                                   >  2:3818795/1‑93 (MQ=255)
         gTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAgg                                                                               <  1:5705369/93‑1 (MQ=255)
          tCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAggg                                                                              >  2:1772494/1‑93 (MQ=255)
           cAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCTCGGGCATTACGAAAAAGTAACCCTCCATCCAGGGc                                                                             >  1:852546/1‑93 (MQ=255)
            aGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCt                                                                            <  2:5447753/93‑1 (MQ=255)
            aGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCt                                                                            <  1:3050554/93‑1 (MQ=255)
             gcgcCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTa                                                                           >  2:5627219/1‑93 (MQ=255)
               gcagggAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTAcc                                                                         <  2:4624926/90‑1 (MQ=255)
               gcCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTAcc                                                                         >  2:5986672/1‑93 (MQ=255)
               gcCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTAcc                                                                         >  1:6141486/1‑93 (MQ=255)
               gcCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTAcc                                                                         >  2:157707/1‑93 (MQ=255)
                 cGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTg                                                                       >  2:2529475/1‑93 (MQ=255)
                  gggAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGa                                                                      >  2:1532022/1‑93 (MQ=255)
                  gggAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGCTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGa                                                                      >  2:26209/1‑93 (MQ=255)
                   ggAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAc                                                                     >  1:3588490/1‑93 (MQ=255)
                    gAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAca                                                                    >  2:1005965/1‑93 (MQ=255)
                    gAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAca                                                                    >  1:2920624/1‑93 (MQ=255)
                    gAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAca                                                                    >  1:2239005/1‑93 (MQ=255)
                     aaCGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcat                                                                   <  1:700400/93‑1 (MQ=255)
                      aCGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatc                                                                  >  2:5052543/1‑93 (MQ=255)
                       cGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatca                                                                 <  2:2685307/93‑1 (MQ=255)
                       cGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatca                                                                 >  2:1009990/1‑93 (MQ=255)
                        gTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTGCCTGAcatcat                                                                <  1:5333120/93‑1 (MQ=255)
                        gTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatcat                                                                <  1:4624172/93‑1 (MQ=255)
                         tttATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACGCTCCATCCAGGGCTACCTGAcatcata                                                               >  2:1248563/1‑92 (MQ=255)
                         tttATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACatcatc                                                               <  2:3441018/93‑1 (MQ=255)
                         tttATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACatcatc                                                               >  2:2064621/1‑93 (MQ=255)
                         tttATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACatcatc                                                               >  1:5375858/1‑93 (MQ=255)
                          ttattaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACAtcatca                                                              >  1:4724445/1‑93 (MQ=255)
                          ttattaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACAtcatca                                                              >  1:5268129/1‑93 (MQ=255)
                           tattaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCaa                                                             <  1:6045735/93‑1 (MQ=255)
                            attaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAag                                                            >  2:2099907/1‑93 (MQ=255)
                             ttaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagc                                                           >  1:3936728/1‑93 (MQ=255)
                             ttaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagc                                                           >  2:3769201/1‑93 (MQ=255)
                             ttaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagc                                                           >  1:4083579/1‑93 (MQ=255)
                              taGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagca                                                          >  1:2279456/1‑93 (MQ=255)
                              taGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagca                                                          >  2:1444464/1‑93 (MQ=255)
                                gAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagcagc                                                        >  1:4334279/1‑93 (MQ=255)
                                gAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagcagc                                                        >  1:4118863/1‑93 (MQ=255)
                                gAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagcagc                                                        >  1:864952/1‑93 (MQ=255)
                                 aaaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGcc                                                       <  2:5303526/93‑1 (MQ=255)
                                  aaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCt                                                      >  1:26481/1‑93 (MQ=255)
                                  aaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCt                                                      >  1:4823303/1‑93 (MQ=255)
                                  aaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCt                                                      <  2:5514893/93‑1 (MQ=255)
                                    aataaataAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGa                                                    <  2:2319775/93‑1 (MQ=255)
                                     ataaataaAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAt                                                   <  2:609107/93‑1 (MQ=255)
                                     ataaataaAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAt                                                   >  1:127964/1‑93 (MQ=255)
                                      taaataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAtt                                                  >  2:4424715/1‑93 (MQ=255)
                                       aaataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTc                                                 <  2:4269677/93‑1 (MQ=255)
                                       aaataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTc                                                 <  2:3164580/93‑1 (MQ=255)
                                        aataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCa                                                >  1:1944427/1‑93 (MQ=255)
                                        aataaaACCAGCGTTGGGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTAGCTGACATCATCAAGCAGTCTGATTCa                                                >  1:781802/1‑93 (MQ=255)
                                         ataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAg                                               >  2:5750077/1‑93 (MQ=255)
                                          taaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGa                                              >  1:5625649/1‑93 (MQ=255)
                                          taaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGa                                              >  2:2018541/1‑93 (MQ=255)
                                          taaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATAATCAAGCAGCCTGATTCAGa                                              >  1:4680837/1‑93 (MQ=255)
                                            aaaCCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGaaa                                            <  2:273369/93‑1 (MQ=255)
                                               ccAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTc                                         >  2:6014660/1‑93 (MQ=255)
                                                 aGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCaa                                       <  2:1871432/93‑1 (MQ=255)
                                                  gCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCaaa                                      >  1:1604475/1‑93 (MQ=255)
                                                     ttGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATg                                   >  1:662432/1‑93 (MQ=255)
                                                     ttGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATg                                   >  1:4713147/1‑93 (MQ=255)
                                                     ttGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATg                                   >  1:5539355/1‑93 (MQ=255)
                                                     ttGTGTTTGAAACTCACGGGAATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTAAAAATg                                   >  1:26153/1‑93 (MQ=255)
                                                      tgtgTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGt                                  >  2:5086437/1‑93 (MQ=255)
                                                       gtgtTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTa                                 <  2:2177960/93‑1 (MQ=255)
                                                       gtgtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTa                                 <  2:3658713/93‑1 (MQ=255)
                                                       gtgtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTa                                 >  2:1636752/1‑93 (MQ=255)
                                                       gtgtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTa                                 <  2:1728904/93‑1 (MQ=255)
                                                        tgtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTAc                                >  2:2586047/1‑93 (MQ=255)
                                                        tgtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTAc                                <  1:385105/93‑1 (MQ=255)
                                                         gtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACt                               >  2:4103693/1‑93 (MQ=255)
                                                          tttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTc                              >  1:4778435/1‑93 (MQ=255)
                                                          tttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTc                              >  2:4817571/1‑93 (MQ=255)
                                                           ttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCg                             <  2:2851455/93‑1 (MQ=255)
                                                              aaaCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTg                          >  2:3032603/1‑93 (MQ=255)
                                                               aaCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGa                         <  2:1604050/93‑1 (MQ=255)
                                                                aCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGat                        <  1:3993225/93‑1 (MQ=255)
                                                                    gcGGGCATTACGAAAAAGTATCCATCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattata                    <  1:3528522/92‑1 (MQ=255)
                                                                       ggCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatatta                 >  1:2990496/1‑93 (MQ=255)
                                                                       ggCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatatta                 >  2:3152135/1‑93 (MQ=255)
                                                                        gCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatattat                >  1:1192851/1‑93 (MQ=255)
                                                                        gCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatattat                <  2:51260/93‑1 (MQ=255)
                                                                         cATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATc               <  2:4512147/93‑1 (MQ=255)
                                                                         cATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATc               <  2:4914062/93‑1 (MQ=255)
                                                                         cATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATc               >  2:5930460/1‑93 (MQ=255)
                                                                           ttACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGACAAAATGTACTCGCTGATTATAATATAGc             >  2:4138646/1‑93 (MQ=255)
                                                                             aCGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCa           <  1:5074910/93‑1 (MQ=255)
                                                                              cGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCAc          <  2:2971816/93‑1 (MQ=255)
                                                                               gAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACg         >  1:1142032/1‑93 (MQ=255)
                                                                                aaaaaGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGc        >  2:3529298/1‑93 (MQ=255)
                                                                                aaaaaGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGc        >  1:2040761/1‑93 (MQ=255)
                                                                                   aaGTACCCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCta     <  2:584647/93‑1 (MQ=255)
                                                                                   aaGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCta     >  1:2427856/1‑93 (MQ=255)
                                                                                    aGTAACCCTCCAGCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCtat    <  1:4915806/93‑1 (MQ=255)
                                                                                     gTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCtata   >  1:5714955/1‑93 (MQ=255)
                                                                                      tAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATAc  <  1:1495589/93‑1 (MQ=255)
                                                                                          |                                                                                        
AGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATAC  >  NC_000913/3453580‑3453758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  NC_000913/3453577‑3453763
                                                                                             |                                                                                             
CGAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACC                                                                                               >  SRR4302532.1478800/1‑94 (MQ=60)
  AAGCCGTTGAGTCAGCGCCGGTAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCT                                                                                             <  SRR4302532.511513/94‑1 (MQ=60)
  AAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCT                                                                                             >  SRR4302532.1134166/1‑94 (MQ=60)
   AGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTC                                                                                            <  SRR4302532.5076220/94‑1 (MQ=60)
   AGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTC                                                                                            >  SRR4302532.5190193/1‑94 (MQ=60)
   AGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTC                                                                                            <  SRR4302532.3691598/94‑1 (MQ=60)
    GCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCC                                                                                           <  SRR4302532.678928/94‑1 (MQ=60)
    GCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCC                                                                                           <  SRR4302532.5516727/94‑1 (MQ=60)
     CCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCA                                                                                          >  SRR4302532.5142269/1‑94 (MQ=60)
       CTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATC                                                                                        <  SRR4302532.6161915/94‑1 (MQ=60)
       CTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATC                                                                                        >  SRR4302532.3462367/1‑94 (MQ=60)
          GAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAG                                                                                     <  SRR4302532.6300822/94‑1 (MQ=60)
            GTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGG                                                                                   <  SRR4302532.488328/94‑1 (MQ=60)
            GTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGG                                                                                   <  SRR4302532.5904293/94‑1 (MQ=60)
             TCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCTCGGGCATTACGAAAAAGTAACCCTCCATCCAGGGC                                                                                  >  SRR4302532.881856/1‑94 (MQ=60)
              CAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCT                                                                                 <  SRR4302532.1662067/94‑1 (MQ=60)
               AGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTA                                                                                <  SRR4302532.3151253/94‑1 (MQ=60)
                 CGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACC                                                                              >  SRR4302532.6353335/1‑94 (MQ=60)
                  GCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCT                                                                             <  SRR4302532.2667643/94‑1 (MQ=60)
                  GCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCT                                                                             <  SRR4302532.4380261/94‑1 (MQ=60)
                   CCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTG                                                                            <  SRR4302532.3423617/94‑1 (MQ=60)
                   CCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTG                                                                            <  SRR4302532.4949047/94‑1 (MQ=60)
                    cgtgtttgttctTTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTATGAAATAGTAACCCTCCATCTAGGGCAATCTGA                                                                           >  SRR4302532.5682992/13‑94 (MQ=60)
                     GGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAC                                                                          >  SRR4302532.5522891/1‑94 (MQ=60)
                     GGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAC                                                                          >  SRR4302532.3708521/1‑94 (MQ=60)
                     GGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAC                                                                          >  SRR4302532.1275789/1‑94 (MQ=60)
                     GGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAC                                                                          >  SRR4302532.1022926/1‑94 (MQ=60)
                      GGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACA                                                                         >  SRR4302532.2313304/1‑94 (MQ=60)
                      GGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACA                                                                         >  SRR4302532.3017879/1‑94 (MQ=60)
                       GAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACAT                                                                        <  SRR4302532.5774000/94‑1 (MQ=60)
                        AACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATC                                                                       <  SRR4302532.724357/94‑1 (MQ=60)
                         ACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCA                                                                      >  SRR4302532.3054694/1‑94 (MQ=60)
                           GTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTGCCTGACATCATC                                                                    <  SRR4302532.5518074/94‑1 (MQ=60)
                           GTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATC                                                                    <  SRR4302532.4781843/94‑1 (MQ=60)
                           GTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATC                                                                    >  SRR4302532.5562093/1‑94 (MQ=60)
                            TTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCA                                                                   >  SRR4302532.5449788/1‑94 (MQ=60)
                            TTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCA                                                                   >  SRR4302532.4885660/1‑94 (MQ=60)
                             TTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAA                                                                  <  SRR4302532.643353/94‑1 (MQ=60)
                             GTATTAGAAAAATAAATAAAACCAGCGTTGTGTTGGAAACTCACGGGCACTACGAAAAAGAAACCCTCCATCCAGGGCGACCTGACACCATCAA                                                                  >  SRR4302532.4928333/1‑94 (MQ=60)
                              TATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAG                                                                 >  SRR4302532.1206049/1‑94 (MQ=60)
                              TATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAG                                                                 <  SRR4302532.6254480/94‑1 (MQ=60)
                               ATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGC                                                                >  SRR4302532.4220682/1‑94 (MQ=60)
                               ATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGC                                                                >  SRR4302532.4069379/1‑94 (MQ=60)
                                TTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCA                                                               >  SRR4302532.2355113/1‑94 (MQ=60)
                                TTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCA                                                               <  SRR4302532.2136171/94‑1 (MQ=60)
                                  AGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGC                                                             >  SRR4302532.4480706/1‑94 (MQ=60)
                                  AGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGC                                                             >  SRR4302532.894616/1‑94 (MQ=60)
                                  AGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGC                                                             >  SRR4302532.4257096/1‑94 (MQ=60)
                                    AAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTCCCTGACATCATCAAGCAGCCT                                                           <  SRR4302532.5326702/94‑1 (MQ=60)
                                    AAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCT                                                           >  SRR4302532.27527/1‑94 (MQ=60)
                                    AAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCT                                                           >  SRR4302532.4989348/1‑94 (MQ=60)
                                     AAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTG                                                          <  SRR4302532.4792299/94‑1 (MQ=60)
                                      AAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGA                                                         >  SRR4302532.4646620/1‑94 (MQ=60)
                                       AATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAT                                                        >  SRR4302532.132288/1‑94 (MQ=60)
                                          AAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCA                                                     >  SRR4302532.2009080/1‑94 (MQ=60)
                                          AAATAAAACCAGCGTTGGGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTAGCTGACATCATCAAGCAGTCTGATTCA                                                     >  SRR4302532.808668/1‑94 (MQ=60)
                                            ATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGA                                                   >  SRR4302532.5820870/1‑94 (MQ=60)
                                            ATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATAATCAAGCAGCCTGATTCAGA                                                   >  SRR4302532.4840451/1‑94 (MQ=60)
                                             TAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAA                                                  >  SRR4302532.2551686/1‑94 (MQ=60)
                                               AAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAG                                                >  SRR4302532.350815/1‑94 (MQ=60)
                                               AAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAG                                                >  SRR4302532.577294/1‑94 (MQ=60)
                                                AACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATgatgtcagg                                               <  SRR4302532.1044330/94‑10 (MQ=60)
                                                AACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGT                                               <  SRR4302532.718971/94‑1 (MQ=60)
                                                 ACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTC                                              >  SRR4302532.5639933/1‑94 (MQ=60)
                                                  CCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCCGCCTGCTTCAGAAAGTCA                                             >  SRR4302532.1426069/1‑94 (MQ=60)
                                                  CCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCA                                             >  SRR4302532.1959773/1‑94 (MQ=60)
                                                  CCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGACA                                             >  SRR4302532.4841946/1‑94 (MQ=60)
                                                    AGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAA                                           >  SRR4302532.1658789/1‑94 (MQ=60)
                                                    AGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAA                                           >  SRR4302532.1636785/1‑94 (MQ=60)
                                                      CGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAAT                                         >  SRR4302532.928077/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATG                                        >  SRR4302532.4873967/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATG                                        >  SRR4302532.685342/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATG                                        >  SRR4302532.6085241/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATG                                        >  SRR4302532.5911216/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATG                                        >  SRR4302532.27187/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATG                                        >  SRR4302532.5731602/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAAACCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATG                                        >  SRR4302532.3860288/1‑94 (MQ=60)
                                                       GTTGTGTTTGAAACTCACGGGAATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTAAAAATG                                        >  SRR4302532.27186/1‑94 (MQ=60)
                                                          GTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTAC                                     <  SRR4302532.4196597/94‑1 (MQ=60)
                                                          GTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTAC                                     >  SRR4302532.4004079/1‑94 (MQ=60)
                                                           TGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACT                                    <  SRR4302532.2351516/94‑1 (MQ=60)
                                                           TGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACT                                    <  SRR4302532.398582/94‑1 (MQ=60)
                                                            GTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTC                                   >  SRR4302532.4942993/1‑94 (MQ=60)
                                                             TTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCG                                  <  SRR4302532.3168319/94‑1 (MQ=60)
                                                                   ACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATT                            >  SRR4302532.4843388/1‑94 (MQ=60)
                                                                   ACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATT                            <  SRR4302532.4127724/94‑1 (MQ=60)
                                                                    CTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTA                           <  SRR4302532.2483941/94‑1 (MQ=60)
                                                                    CTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTA                           >  SRR4302532.4130754/1‑94 (MQ=60)
                                                                     TCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTAT                          <  SRR4302532.830066/94‑1 (MQ=60)
                                                                       GCGGGCATTACGAAAAAGTATCCATCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATAT                        <  SRR4302532.3646629/94‑1 (MQ=60)
                                                                       CCGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATAT                        <  SRR4302532.2964854/94‑1 (MQ=60)
                                                                       ACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATAT                        >  SRR4302532.6233629/1‑94 (MQ=60)
                                                                         GGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTA                      >  SRR4302532.3089330/1‑94 (MQ=60)
                                                                          GGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTAT                     <  SRR4302532.1173012/94‑1 (MQ=60)
                                                                          GGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTAT                     >  SRR4302532.1232736/1‑94 (MQ=60)
                                                                                ACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCTC               <  SRR4302532.3119754/94‑1 (MQ=60)
                                                                                ACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCAC               <  SRR4302532.5249366/94‑1 (MQ=60)
                                                                                 CGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACG              >  SRR4302532.1180120/1‑94 (MQ=60)
                                                                                 CGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACG              >  SRR4302532.412637/1‑94 (MQ=60)
                                                                                  GAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGC             >  SRR4302532.2108732/1‑94 (MQ=60)
                                                                                     AAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTA          <  SRR4302532.5893284/94‑1 (MQ=60)
                                                                                     AAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTA          >  SRR4302532.2508551/1‑94 (MQ=60)
                                                                                       AGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATA        >  SRR4302532.5914082/1‑94 (MQ=60)
                                                                                       AGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATA        >  SRR4302532.2075785/1‑94 (MQ=60)
                                                                                       AGTAACCCTCCAGCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATA        <  SRR4302532.5084679/94‑1 (MQ=60)
                                                                                        GTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATAC       <  SRR4302532.4241427/94‑1 (MQ=60)
                                                                                         TAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACG      <  SRR4302532.1546035/94‑1 (MQ=60)
                                                                                         TAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACG      <  SRR4302532.886481/94‑1 (MQ=60)
                                                                                         TAACACTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACG      <  SRR4302532.778821/94‑1 (MQ=60)
                                                                                           ACCCTCCATCCGGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAGATGTACTCGCTGATTATATTATCGCCACGCATATACGTG    >  SRR4302532.1539985/1‑94 (MQ=60)
                                                                                           ACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTG    >  SRR4302532.5746733/1‑94 (MQ=60)
                                                                                            CCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGA   <  SRR4302532.3260789/94‑1 (MQ=60)
                                                                                             CCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  <  SRR4302532.2129551/94‑1 (MQ=60)
                                                                                             CCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  SRR4302532.4442197/1‑94 (MQ=60)
                                                                                             CCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  SRR4302532.2533013/1‑94 (MQ=60)
                                                                                             CCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  SRR4302532.2668676/1‑94 (MQ=60)
                                                                                             CCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  SRR4302532.2668677/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  NC_000913/3453577‑3453763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑