Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,453,670 T→C T152T (ACT→ACC)  yheE → YheE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,453,6700TC100.0% 796.8 / NA 215T152T (ACT→ACC) yheEYheE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (100/115);  total (100/115)

Only 100 of 217 total aligned reads displayed.

GCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACG  >  NC_000913/3453581‑3453759
                                                                                         |                                                                                         
  ccTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtcca                                                                                      <  1:3998251/93‑1 (MQ=255)
   cTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtccat                                                                                     >  2:747348/1‑93 (MQ=255)
     tGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtccatcc                                                                                   >  1:4417347/1‑93 (MQ=255)
     tGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtccatcc                                                                                   <  1:1743241/93‑1 (MQ=255)
      gAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCtccatcca                                                                                  <  2:3595912/93‑1 (MQ=255)
       aGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAg                                                                                 >  1:6581692/1‑93 (MQ=255)
       aGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTCGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAg                                                                                 <  1:5404308/93‑1 (MQ=255)
       aGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTCGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAg                                                                                 <  2:1212448/93‑1 (MQ=255)
         tCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAggg                                                                               <  2:4668652/93‑1 (MQ=255)
          cAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGc                                                                              <  1:31574/93‑1 (MQ=255)
          cAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGc                                                                              <  1:3021116/93‑1 (MQ=255)
           aGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCt                                                                             >  1:264655/1‑93 (MQ=255)
             cgcCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTAc                                                                           <  2:901242/93‑1 (MQ=255)
               ccGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCt                                                                         >  1:754954/1‑93 (MQ=255)
               ccGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCt                                                                         <  1:336361/93‑1 (MQ=255)
               ccGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCt                                                                         <  1:3870687/93‑1 (MQ=255)
                cGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTg                                                                        >  1:910810/1‑93 (MQ=255)
                   gAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAca                                                                     >  1:6308364/1‑93 (MQ=255)
                    aaCGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcat                                                                    >  2:1804702/1‑93 (MQ=255)
                    aaCGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcat                                                                    >  2:3888966/1‑93 (MQ=255)
                      cGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatca                                                                  <  1:4224778/93‑1 (MQ=255)
                       gTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatcat                                                                 <  2:2070737/93‑1 (MQ=255)
                       gTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatcat                                                                 <  2:5196422/93‑1 (MQ=255)
                       gTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatcat                                                                 <  1:1147040/93‑1 (MQ=255)
                       gTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAcatcat                                                                 <  1:2107005/93‑1 (MQ=255)
                         ttattaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACAtcatca                                                               <  1:2005485/93‑1 (MQ=255)
                         ttattaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTAACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACAtcatca                                                               <  2:5255709/93‑1 (MQ=255)
                          tattaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCaa                                                              >  2:1491441/1‑93 (MQ=255)
                           attaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAag                                                             >  1:1446920/1‑93 (MQ=255)
                            ttaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagc                                                            >  1:4575777/1‑93 (MQ=255)
                            ttaGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagc                                                            >  1:5764964/1‑93 (MQ=255)
                             taGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagca                                                           <  2:2311744/93‑1 (MQ=255)
                              aGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagcag                                                          <  2:333007/93‑1 (MQ=255)
                              aGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagcag                                                          >  2:1844025/1‑93 (MQ=255)
                               gAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagcagc                                                         >  1:2834535/1‑93 (MQ=255)
                               gAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAagcagc                                                         >  2:157329/1‑93 (MQ=255)
                                aaaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGcc                                                        >  1:1592458/1‑93 (MQ=255)
                                 aaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCt                                                       >  2:3274630/1‑93 (MQ=255)
                                 aaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCt                                                       <  2:2568800/93‑1 (MQ=255)
                                 aaaaTAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCt                                                       >  2:6219989/1‑93 (MQ=255)
                                  aaataaatAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTg                                                      <  1:5900096/93‑1 (MQ=255)
                                   aataaataAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGa                                                     >  2:6625448/1‑93 (MQ=255)
                                   aataaataAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGa                                                     <  2:4208469/93‑1 (MQ=255)
                                     taaataaaCCCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAtt                                                   <  1:5165182/93‑1 (MQ=255)
                                     taaataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAtt                                                   >  2:165564/1‑93 (MQ=255)
                                      aaataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTc                                                  <  2:4755481/93‑1 (MQ=255)
                                       aataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCa                                                 >  2:3684330/1‑93 (MQ=255)
                                       aataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCa                                                 <  2:1689106/93‑1 (MQ=255)
                                       aataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCa                                                 >  1:3512407/1‑93 (MQ=255)
                                       aataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCa                                                 <  2:6379713/93‑1 (MQ=255)
                                       aataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCa                                                 <  1:4955559/93‑1 (MQ=255)
                                       aataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCa                                                 >  1:4596104/1‑93 (MQ=255)
                                        ataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAg                                                <  2:6162568/93‑1 (MQ=255)
                                        ataaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAg                                                <  1:5441480/93‑1 (MQ=255)
                                         taaaACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGa                                               >  2:4297622/1‑93 (MQ=255)
                                           aaaCCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGaaa                                             <  2:5678315/93‑1 (MQ=255)
                                            aaCCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAg                                            >  1:4749260/1‑93 (MQ=255)
                                             aCCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGt                                           <  2:3089569/93‑1 (MQ=255)
                                              ccAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTc                                          <  1:3781352/93‑1 (MQ=255)
                                              ccAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTc                                          <  1:4702453/93‑1 (MQ=255)
                                              ccAGCGTTGTGTTTGAAACCCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTc                                          >  1:6482755/1‑93 (MQ=255)
                                               cAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCa                                         >  2:4515723/1‑93 (MQ=255)
                                               cAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCa                                         >  1:5067569/1‑93 (MQ=255)
                                                aGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCaa                                        >  2:2688718/1‑93 (MQ=255)
                                                 gCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCaaa                                       >  1:2045049/1‑93 (MQ=255)
                                                 gCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCaaa                                       <  2:3526082/93‑1 (MQ=255)
                                                  cGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCaaaa                                      <  2:1982470/93‑1 (MQ=255)
                                                      gtgtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTa                                  <  2:459949/93‑1 (MQ=255)
                                                      gtgtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTa                                  <  1:3865814/93‑1 (MQ=255)
                                                        gtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACt                                >  2:5958356/1‑93 (MQ=255)
                                                        gtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACt                                <  2:1638533/93‑1 (MQ=255)
                                                        gtTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACt                                >  1:102964/1‑93 (MQ=255)
                                                         tttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTc                               >  1:898670/1‑93 (MQ=255)
                                                          ttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCg                              >  1:2234479/1‑93 (MQ=255)
                                                          ttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCg                              >  2:4002959/1‑93 (MQ=255)
                                                          ttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCg                              >  1:5875179/1‑93 (MQ=255)
                                                          ttGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCg                              >  2:4685910/1‑93 (MQ=255)
                                                           tGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGc                             >  2:5056944/1‑93 (MQ=255)
                                                             aaaCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTg                           >  2:2890033/1‑93 (MQ=255)
                                                              aaCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGa                          <  1:4266944/93‑1 (MQ=255)
                                                               aCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGat                         >  1:4501861/1‑93 (MQ=255)
                                                               aCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGat                         <  2:1866031/93‑1 (MQ=255)
                                                               aCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGat                         <  2:531719/93‑1 (MQ=255)
                                                                cTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGatt                        <  1:5851365/93‑1 (MQ=255)
                                                                   aCGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattata                     <  2:5764977/93‑1 (MQ=255)
                                                                   aCGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattata                     <  2:5621019/93‑1 (MQ=255)
                                                                    cGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatat                    >  2:812143/1‑93 (MQ=255)
                                                                     gggCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatatt                   >  2:6408618/1‑93 (MQ=255)
                                                                     gggCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatatt                   <  2:4363460/93‑1 (MQ=255)
                                                                       gCATTACGAAAAAGTACCCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGattatattat                 <  1:5516200/93‑1 (MQ=255)
                                                                        cATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATc                <  2:3546746/93‑1 (MQ=255)
                                                                        cATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATc                <  2:2746468/93‑1 (MQ=255)
                                                                        cATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATc                <  1:2797337/93‑1 (MQ=255)
                                                                         aTTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCg               <  2:4875347/93‑1 (MQ=255)
                                                                          ttACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGc              <  2:2962635/93‑1 (MQ=255)
                                                                          ttACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGc              >  1:234549/1‑93 (MQ=255)
                                                                          ttACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGc              <  2:651533/93‑1 (MQ=255)
                                                                            aCGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCa            <  1:5735330/93‑1 (MQ=255)
                                                                                 aaaGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCt       >  1:1902710/1‑93 (MQ=255)
                                                                                      aaCCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACg  >  2:4266052/1‑93 (MQ=255)
                                                                                         |                                                                                         
GCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACG  >  NC_000913/3453581‑3453759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  NC_000913/3453577‑3453763
                                                                                             |                                                                                             
CGAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACC                                                                                               >  SRR4302547.5202611/1‑94 (MQ=60)
 GAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCC                                                                                              <  SRR4302547.4664571/94‑1 (MQ=60)
 GAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCC                                                                                              >  SRR4302547.4856589/1‑94 (MQ=60)
 GAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCC                                                                                              >  SRR4302547.1078860/1‑94 (MQ=60)
 GAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCC                                                                                              <  SRR4302547.5504593/94‑1 (MQ=60)
 GAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAAACAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCC                                                                                              >  SRR4302547.5266051/1‑94 (MQ=60)
 GAAGCCATTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCC                                                                                              >  SRR4302547.5092005/1‑94 (MQ=60)
  AAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCT                                                                                             <  SRR4302547.1016765/94‑1 (MQ=60)
     CCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCA                                                                                          >  SRR4302547.5846342/1‑94 (MQ=60)
      CCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCAT                                                                                         <  SRR4302547.4079527/94‑1 (MQ=60)
      CCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCAT                                                                                         <  SRR4302547.4829803/94‑1 (MQ=60)
        TTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCC                                                                                       <  SRR4302547.212689/94‑1 (MQ=60)
        TTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCC                                                                                       >  SRR4302547.4508508/1‑94 (MQ=60)
         TGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCA                                                                                      <  SRR4302547.1777577/94‑1 (MQ=60)
         TGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGAGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCAA                                                                                      >  SRR4302547.4585185/1‑94 (MQ=60)
          GAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAG                                                                                     >  SRR4302547.6718748/1‑94 (MQ=60)
           AGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGG                                                                                    <  SRR4302547.1469805/94‑1 (MQ=60)
           AGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTCGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGG                                                                                    <  SRR4302547.5516015/94‑1 (MQ=60)
              CAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCT                                                                                 <  SRR4302547.32134/94‑1 (MQ=60)
              CAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCT                                                                                 <  SRR4302547.3081193/94‑1 (MQ=60)
              CAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCT                                                                                 >  SRR4302547.269601/1‑94 (MQ=60)
                  GCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCT                                                                             >  SRR4302547.769182/1‑94 (MQ=60)
                   CCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTG                                                                            >  SRR4302547.928364/1‑94 (MQ=60)
                   CCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTG                                                                            <  SRR4302547.3949475/94‑1 (MQ=60)
                   CCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTG                                                                            <  SRR4302547.342621/94‑1 (MQ=60)
                    CGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGA                                                                           >  SRR4302547.2808980/1‑94 (MQ=60)
                     GGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGAC                                                                          >  SRR4302547.6003495/1‑94 (MQ=60)
                      GGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACA                                                                         >  SRR4302547.3718247/1‑94 (MQ=60)
                      GGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACA                                                                         >  SRR4302547.6439512/1‑94 (MQ=60)
                       GAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACAT                                                                        >  SRR4302547.6505125/1‑94 (MQ=60)
                        AACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATC                                                                       <  SRR4302547.2257447/94‑1 (MQ=60)
                          CGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCAT                                                                     >  SRR4302547.3978935/1‑94 (MQ=60)
                          CGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCAT                                                                     <  SRR4302547.4311097/94‑1 (MQ=60)
                           GTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATC                                                                    <  SRR4302547.2148983/94‑1 (MQ=60)
                           GTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATC                                                                    <  SRR4302547.1170233/94‑1 (MQ=60)
                             TTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAA                                                                  <  SRR4302547.2045392/94‑1 (MQ=60)
                              TATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAG                                                                 >  SRR4302547.1475857/1‑94 (MQ=60)
                               ATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGC                                                                >  SRR4302547.4670491/1‑94 (MQ=60)
                               ATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGC                                                                >  SRR4302547.5884194/1‑94 (MQ=60)
                                TTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCA                                                               >  SRR4302547.2063493/1‑94 (MQ=60)
                                 TAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAG                                                              <  SRR4302547.2042915/94‑1 (MQ=60)
                                  AGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGC                                                             >  SRR4302547.2891212/1‑94 (MQ=60)
                                  AGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGC                                                             >  SRR4302547.4618854/1‑94 (MQ=60)
                                   GAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCC                                                            >  SRR4302547.1624079/1‑94 (MQ=60)
                                    AAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCT                                                           <  SRR4302547.5988763/94‑1 (MQ=60)
                                      AAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGA                                                         >  SRR4302547.2036493/1‑94 (MQ=60)
                                      AAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGA                                                         <  SRR4302547.3947423/94‑1 (MQ=60)
                                      AAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGA                                                         <  SRR4302547.6022277/94‑1 (MQ=60)
                                      AAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGA                                                         >  SRR4302547.4740133/1‑94 (MQ=60)
                                       AATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTGACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAT                                                        <  SRR4302547.5215083/94‑1 (MQ=60)
                                       AATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAT                                                        <  SRR4302547.5378076/94‑1 (MQ=60)
                                       AATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAT                                                        <  SRR4302547.4690721/94‑1 (MQ=60)
                                       AATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGAT                                                        >  SRR4302547.5537663/1‑94 (MQ=60)
                                        ATAAATAAACCCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATT                                                       <  SRR4302547.840857/94‑1 (MQ=60)
                                        ATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATT                                                       >  SRR4302547.4387828/1‑94 (MQ=60)
                                         TAAATAAACCCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTC                                                      <  SRR4302547.5271523/94‑1 (MQ=60)
                                          AAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCA                                                     >  SRR4302547.4691207/1‑94 (MQ=60)
                                          AAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCA                                                     >  SRR4302547.3584111/1‑94 (MQ=60)
                                           AATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAG                                                    <  SRR4302547.5058133/94‑1 (MQ=60)
                                           AATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAG                                                    <  SRR4302547.6703024/94‑1 (MQ=60)
                                            ATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGA                                                   <  SRR4302547.5553924/94‑1 (MQ=60)
                                             TAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAA                                                  >  SRR4302547.1688241/1‑94 (MQ=60)
                                               AAACCAGCGTTTTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAG                                                <  SRR4302547.4194320/94‑1 (MQ=60)
                                               AAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAG                                                >  SRR4302547.4847671/1‑94 (MQ=60)
                                                AACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGT                                               >  SRR4302547.3570272/1‑94 (MQ=60)
                                                 ACCAGCGTTGTGTTTGAAACTCACTGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGGC                                              >  SRR4302547.842060/1‑94 (MQ=60)
                                                 ACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTC                                              <  SRR4302547.5957791/94‑1 (MQ=60)
                                                 ACCAGCGTTGTGTTTGAAACCCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTC                                              >  SRR4302547.6617717/1‑94 (MQ=60)
                                                  CCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCA                                             <  SRR4302547.4799842/94‑1 (MQ=60)
                                                  CCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCA                                             <  SRR4302547.5613476/94‑1 (MQ=60)
                                                  CCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCA                                             <  SRR4302547.3858449/94‑1 (MQ=60)
                                                  CCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCA                                             >  SRR4302547.5172130/1‑94 (MQ=60)
                                                    AGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAA                                           >  SRR4302547.2085841/1‑94 (MQ=60)
                                                    AGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAA                                           >  SRR4302547.4315775/1‑94 (MQ=60)
                                                          GTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTAC                                     <  SRR4302547.3944491/94‑1 (MQ=60)
                                                          GTGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTAC                                     <  SRR4302547.4984221/94‑1 (MQ=60)
                                                           TGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACT                                    >  SRR4302547.104847/1‑94 (MQ=60)
                                                           TGTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACT                                    >  SRR4302547.192984/1‑94 (MQ=60)
                                                            GTTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTC                                   >  SRR4302547.915946/1‑94 (MQ=60)
                                                             TTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCG                                  >  SRR4302547.5996800/1‑94 (MQ=60)
                                                             TTTGAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCG                                  >  SRR4302547.2279525/1‑94 (MQ=60)
                                                                GAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTG                               <  SRR4302547.6430847/94‑1 (MQ=60)
                                                                GAAACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTG                               >  SRR4302547.3908786/1‑94 (MQ=60)
                                                                  ATCTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGAT                             <  SRR4302547.2652884/94‑1 (MQ=60)
                                                                  AACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGAT                             <  SRR4302547.2882640/94‑1 (MQ=60)
                                                                  AACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGAT                             <  SRR4302547.4354409/94‑1 (MQ=60)
                                                                  AACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGAT                             <  SRR4302547.1513398/94‑1 (MQ=60)
                                                                  AACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGAT                             <  SRR4302547.5076431/94‑1 (MQ=60)
                                                                  AACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGAT                             >  SRR4302547.4594838/1‑94 (MQ=60)
                                                                  AACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGAT                             <  SRR4302547.3641062/94‑1 (MQ=60)
                                                                   ACTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATT                            >  SRR4302547.3395967/1‑94 (MQ=60)
                                                                    CTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTA                           >  SRR4302547.6448492/1‑94 (MQ=60)
                                                                    CTCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTA                           <  SRR4302547.5972404/94‑1 (MQ=60)
                                                                    CTCACGGGCATTACGAAAAAGTAACCCTACATCCAGGGTTAACTGACATCATCAAGCAGCCTGATTCAGAAAGACAAAATGTACTTGCTGATTA                           >  SRR4302547.4383337/1‑94 (MQ=60)
                                                                     TCACGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTAT                          <  SRR4302547.586062/94‑1 (MQ=60)
                                                                        CGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATT                       >  SRR4302547.1065228/1‑94 (MQ=60)
                                                                        CGGGCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATT                       >  SRR4302547.2967050/1‑94 (MQ=60)
                                                                           GCATTACGAAAAAGTACCCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATC                    <  SRR4302547.5630081/94‑1 (MQ=60)
                                                                           GCATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATC                    <  SRR4302547.923100/94‑1 (MQ=60)
                                                                            CATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCG                   >  SRR4302547.3132103/1‑94 (MQ=60)
                                                                            CATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCG                   <  SRR4302547.2853334/94‑1 (MQ=60)
                                                                            CATTACGAAAAAGAAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCACTGATTATATTATCA                   >  SRR4302547.678358/1‑94 (MQ=60)
                                                                             ATTACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGC                  >  SRR4302547.238957/1‑94 (MQ=60)
                                                                               TACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCA                <  SRR4302547.2442217/94‑1 (MQ=60)
                                                                                ACGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCAC               <  SRR4302547.5853818/94‑1 (MQ=60)
                                                                                 CGAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACG              >  SRR4302547.2318496/1‑94 (MQ=60)
                                                                                  GAAAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGATAGTCAAAATGTACTCGCTGATTATATTATCGCCACGC             >  SRR4302547.2750869/1‑94 (MQ=60)
                                                                                    AAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCT           >  SRR4302547.1940351/1‑94 (MQ=60)
                                                                                    AAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCT           <  SRR4302547.485285/94‑1 (MQ=60)
                                                                                    AAAAGTAACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCT           >  SRR4302547.5651646/1‑94 (MQ=60)
                                                                                         TAACCCTCCATCCAGGGCTGCCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACG      <  SRR4302547.4446820/94‑1 (MQ=60)
                                                                                          AACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGT     >  SRR4302547.6468468/1‑94 (MQ=60)
                                                                                          AACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGT     >  SRR4302547.4696101/1‑94 (MQ=60)
                                                                                           ACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTG    >  SRR4302547.6447602/1‑94 (MQ=60)
                                                                                           ACCCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTG    >  SRR4302547.4123897/1‑94 (MQ=60)
                                                                                             CCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  SRR4302547.2863404/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGAAGCCCTTGAGTCAGCGCCGGGAACGTTTATTAGAAAAATAAATAAAACCAGCGTTGTGTTTGAAACTCACGGGCATTACGAAAAAGTAACTCTCCATCCAGGGCTACCTGACATCATCAAGCAGCCTGATTCAGAAAGTCAAAATGTACTCGCTGATTATATTATCGCCACGCCTATACGTGAT  >  NC_000913/3453577‑3453763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑