Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,673,861 A→G intergenic (+123/+59) yhjE → / ← yhjG putative transport protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,673,8610AG84.0% 815.9 / 138.7 331intergenic (+123/+59)yhjE/yhjGputative transport protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/21);  new base G (149/129);  total (181/150)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

Only 100 of 342 total aligned reads displayed.

TCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGAC  >  NC_000913/3673770‑3673951
                                                                                           |                                                                                          
 cAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCt                                                                                          >  2:3012578/1‑93 (MQ=255)
  aGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACtt                                                                                         <  1:3824452/93‑1 (MQ=255)
  aGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACtt                                                                                         >  2:3519319/1‑93 (MQ=255)
   ggCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCttt                                                                                        >  1:4613427/1‑93 (MQ=255)
   ggCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCATGAACAAAGCACGCttt                                                                                        >  1:4484560/1‑93 (MQ=255)
    gCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTg                                                                                       >  1:5439854/1‑93 (MQ=255)
    gCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTg                                                                                       >  2:4502749/1‑93 (MQ=255)
     ccTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCttt                                                                                        <  1:6618906/91‑1 (MQ=255)
     ccTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGc                                                                                      >  1:5255635/1‑93 (MQ=255)
      cTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGTCATGAACAAAGCACACTTTGcc                                                                                     <  1:3270379/93‑1 (MQ=255)
      cTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGcc                                                                                     <  1:2462275/93‑1 (MQ=255)
        gAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCcaa                                                                                   >  2:1576672/1‑93 (MQ=255)
        gAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCcaa                                                                                   <  1:6192346/93‑1 (MQ=255)
        gAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaa                                                                                   >  2:5481536/1‑93 (MQ=255)
           tttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCATGAACAAAGCACGCTTTGCcaacaa                                                                                >  1:5305389/1‑93 (MQ=255)
            ttCCATACAATATATTTAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAAt                                                                               <  1:2394881/93‑1 (MQ=255)
            ttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAAt                                                                               <  1:2561718/93‑1 (MQ=255)
            ttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAAt                                                                               <  2:5747189/93‑1 (MQ=255)
               cATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTa                                                                            >  1:764899/1‑93 (MQ=255)
                 tACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTaaa                                                                          >  2:5834956/1‑93 (MQ=255)
                   cAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATc                                                                        <  2:3907762/93‑1 (MQ=255)
                   cAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATc                                                                        <  1:974397/93‑1 (MQ=255)
                    aaTATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCt                                                                       <  2:1238991/93‑1 (MQ=255)
                      tatatTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGt                                                                     <  1:2364704/93‑1 (MQ=255)
                       atatTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATATGtt                                                                    <  2:2954160/93‑1 (MQ=255)
                        tatTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGttt                                                                   >  1:3661048/1‑93 (MQ=255)
                        tatTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGttt                                                                   >  2:599199/1‑93 (MQ=255)
                         atTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTa                                                                  >  2:3093901/1‑93 (MQ=255)
                         atTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTa                                                                  >  2:2483770/1‑93 (MQ=255)
                          ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTaa                                                                 >  2:5364712/1‑93 (MQ=255)
                           tGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAAt                                                                <  2:877526/93‑1 (MQ=255)
                             aaTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTg                                                              >  2:4225786/1‑93 (MQ=255)
                              aTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGc                                                             <  1:164464/93‑1 (MQ=255)
                              aTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGc                                                             <  2:5541813/93‑1 (MQ=255)
                               ttCGAAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                            <  2:4434838/93‑1 (MQ=255)
                                tCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCg                                                           <  2:3458856/93‑1 (MQ=255)
                                   cAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAt                                                        <  1:4088198/93‑1 (MQ=255)
                                   cAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAt                                                        <  1:3167736/93‑1 (MQ=255)
                                   cAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAt                                                        <  1:6351699/93‑1 (MQ=255)
                                      ggATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTc                                                     >  2:2118545/1‑93 (MQ=255)
                                       gATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCa                                                    >  1:5642160/1‑93 (MQ=255)
                                         tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGa                                                  <  2:2226911/93‑1 (MQ=255)
                                         tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGa                                                  <  1:1068134/93‑1 (MQ=255)
                                          ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGAc                                                 >  2:3733581/1‑93 (MQ=255)
                                           tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACa                                                >  1:1602542/1‑93 (MQ=255)
                                            gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACAt                                               >  1:1028506/1‑93 (MQ=255)
                                            gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACAt                                               >  1:6464124/1‑93 (MQ=255)
                                            gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACAt                                               >  2:493961/1‑93 (MQ=255)
                                              aGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATcc                                             <  2:6190877/93‑1 (MQ=255)
                                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTGAGACATCCg                                            >  1:2135215/1‑93 (MQ=255)
                                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCg                                            >  1:852903/1‑93 (MQ=255)
                                                 ccGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGc                                          <  1:5537986/93‑1 (MQ=255)
                                                  cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCa                                         >  2:4597429/1‑93 (MQ=255)
                                                   ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCaa                                        >  1:5172650/1‑93 (MQ=255)
                                                   ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCaa                                        <  1:1145560/93‑1 (MQ=255)
                                                    gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAt                                       <  2:6249183/93‑1 (MQ=255)
                                                    gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAt                                       >  1:4193073/1‑93 (MQ=255)
                                                    gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAt                                       >  1:1290405/1‑93 (MQ=255)
                                                    gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAt                                       >  1:6558199/1‑93 (MQ=255)
                                                     aTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAtt                                      >  1:5030322/1‑93 (MQ=255)
                                                     aTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAtt                                      >  2:2771032/1‑93 (MQ=255)
                                                      tAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTa                                     <  1:4793907/93‑1 (MQ=255)
                                                         ggCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatca                                  >  1:4654994/1‑93 (MQ=255)
                                                         ggCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatca                                  >  2:6605561/1‑93 (MQ=255)
                                                           cGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatcatc                                >  1:2679491/1‑93 (MQ=255)
                                                           cGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatcatc                                <  1:2794707/93‑1 (MQ=255)
                                                            gTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAtcatca                               >  1:4738419/1‑93 (MQ=255)
                                                            gTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAtcatca                               <  2:3257267/93‑1 (MQ=255)
                                                             ttCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAc                              <  2:6049681/93‑1 (MQ=255)
                                                             ttCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCCGCAATTATCATCAc                              >  2:4716920/1‑93 (MQ=255)
                                                              tCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAct                             <  1:3524694/93‑1 (MQ=255)
                                                               cACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATTActt                            >  1:3423069/1‑93 (MQ=255)
                                                                aCGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCActtt                           >  1:6066641/1‑92 (MQ=255)
                                                                 cgccgcATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCActtct                          <  1:5676107/93‑1 (MQ=255)
                                                                  gccgcATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCActtctt                         >  2:6411984/1‑93 (MQ=255)
                                                                  gccgcATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCActtctt                         >  2:1700964/1‑93 (MQ=255)
                                                                   ccgcATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACttcttc                        <  2:1470691/93‑1 (MQ=255)
                                                                   ccgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACttcttc                        <  1:1777690/93‑1 (MQ=255)
                                                                   ccgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACttcttc                        <  1:1956556/93‑1 (MQ=255)
                                                                    cgcATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCa                       >  1:831265/1‑93 (MQ=255)
                                                                     gcATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAt                      >  2:6095661/1‑93 (MQ=255)
                                                                     gcATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAt                      >  1:4025594/1‑93 (MQ=255)
                                                                     gcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAt                      >  2:1893880/1‑93 (MQ=255)
                                                                       aTCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCt                    <  2:2346764/93‑1 (MQ=255)
                                                                        tCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTg                   >  1:5768226/1‑93 (MQ=255)
                                                                           ggCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGa                <  1:2305403/93‑1 (MQ=255)
                                                                           ggCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGa                >  2:6525959/1‑93 (MQ=255)
                                                                             cATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACa              >  1:436808/1‑93 (MQ=255)
                                                                              aTGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACaa             >  1:1408299/1‑93 (MQ=255)
                                                                               tGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACaaa            >  2:1047000/1‑93 (MQ=255)
                                                                               tGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACaaa            >  2:3134115/1‑93 (MQ=255)
                                                                                gAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACaaaa           >  2:4891513/1‑93 (MQ=255)
                                                                                 aaCAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAAt          <  2:4041228/93‑1 (MQ=255)
                                                                                 aaCAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAAt          <  1:5914122/93‑1 (MQ=255)
                                                                                 aaCAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAAt          >  1:4540468/1‑93 (MQ=255)
                                                                                 aaCAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAAt          <  1:2957637/93‑1 (MQ=255)
                                                                                  aCAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATc         <  1:2197603/93‑1 (MQ=255)
                                                                                     aaGCACGCTTTGCAAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTc      <  2:3216709/93‑1 (MQ=255)
                                                                                      aacacgCTTTGCCAACAATCTAAATCTGTTTAATTGCCCGATGACAGACATCCGACAATTATCATCACTTCTTCTTCTGCGACAAAATCGTcc     >  1:323439/3‑93 (MQ=255)
                                                                                      aGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTcc     <  1:492778/93‑1 (MQ=255)
                                                                                           |                                                                                          
TCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGAC  >  NC_000913/3673770‑3673951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 202 total aligned reads displayed.
ATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACT  >  NC_000913/3673769‑3673954
                                                                                            |                                                                                             
ATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACAC                                                                                              >  SRR4302547.4964183/1‑94 (MQ=60)
ATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACAC                                                                                              >  SRR4302547.3869155/1‑94 (MQ=60)
  CAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTT                                                                                            <  SRR4302547.1065383/94‑1 (MQ=60)
  CAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTT                                                                                            >  SRR4302547.3735102/1‑94 (MQ=60)
  CAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTT                                                                                            >  SRR4302547.866603/1‑94 (MQ=60)
   AGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTT                                                                                           <  SRR4302547.1349872/94‑1 (MQ=60)
   AGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTT                                                                                           >  SRR4302547.4708926/1‑94 (MQ=60)
   AGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTT                                                                                           >  SRR4302547.481191/1‑94 (MQ=60)
   AGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTT                                                                                           >  SRR4302547.4678534/1‑94 (MQ=60)
   AGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTT                                                                                           <  SRR4302547.3902364/94‑1 (MQ=60)
   AGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCATGAACAAAGCACGCTTT                                                                                           >  SRR4302547.4577209/1‑94 (MQ=60)
    TGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTG                                                                                          <  SRR4302547.6756665/94‑1 (MQ=60)
    GGCCTGGATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTG                                                                                          >  SRR4302547.858238/1‑94 (MQ=60)
    GGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTG                                                                                          >  SRR4302547.5552273/1‑94 (MQ=60)
    GGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTG                                                                                          >  SRR4302547.5347186/1‑94 (MQ=60)
     GCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGC                                                                                         >  SRR4302547.5363841/1‑94 (MQ=60)
      CCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCC                                                                                        <  SRR4302547.2723831/94‑1 (MQ=60)
      CCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCC                                                                                        >  SRR4302547.5256944/1‑94 (MQ=60)
       CTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGTCATGAACAAAGCACACTTTGCCA                                                                                       <  SRR4302547.3335848/94‑1 (MQ=60)
       CTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCA                                                                                       <  SRR4302547.2512044/94‑1 (MQ=60)
         GAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAAC                                                                                     >  SRR4302547.5243005/1‑94 (MQ=60)
         GAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAAC                                                                                     <  SRR4302547.6320897/94‑1 (MQ=60)
         GAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAAC                                                                                     <  SRR4302547.2376457/94‑1 (MQ=60)
         GAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAAGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAAC                                                                                     >  SRR4302547.3986045/1‑94 (MQ=60)
          AATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACA                                                                                    <  SRR4302547.551166/94‑1 (MQ=60)
           ATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAA                                                                                   >  SRR4302547.154582/1‑94 (MQ=60)
           ATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAA                                                                                   >  SRR4302547.5414825/1‑94 (MQ=60)
             TTCCATACAATATATTTAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATC                                                                                 <  SRR4302547.2443388/94‑1 (MQ=60)
             TTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATC                                                                                 >  SRR4302547.4195911/1‑94 (MQ=60)
             TTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATC                                                                                 <  SRR4302547.2613444/94‑1 (MQ=60)
              TCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCT                                                                                <  SRR4302547.5045008/94‑1 (MQ=60)
               CCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTA                                                                               >  SRR4302547.3293777/1‑94 (MQ=60)
               CCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTA                                                                               >  SRR4302547.1221458/1‑94 (MQ=60)
               CCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTA                                                                               >  SRR4302547.5723116/1‑94 (MQ=60)
               CCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTA                                                                               >  SRR4302547.779314/1‑94 (MQ=60)
                CATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAA                                                                              <  SRR4302547.4717689/94‑1 (MQ=60)
                CATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAA                                                                              >  SRR4302547.4164006/1‑94 (MQ=60)
                 ATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAA                                                                             <  SRR4302547.2195373/94‑1 (MQ=60)
                 ATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAA                                                                             >  SRR4302547.4863624/1‑94 (MQ=60)
                 ATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAA                                                                             >  SRR4302547.1776189/1‑94 (MQ=60)
                  TACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAAT                                                                            >  SRR4302547.1851110/1‑94 (MQ=60)
                   ACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATC                                                                           <  SRR4302547.3357266/94‑1 (MQ=60)
                    CAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCT                                                                          <  SRR4302547.2347338/94‑1 (MQ=60)
                    CAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCT                                                                          <  SRR4302547.209691/94‑1 (MQ=60)
                    CAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCT                                                                          >  SRR4302547.1890735/1‑94 (MQ=60)
                    CAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCT                                                                          >  SRR4302547.6575003/1‑94 (MQ=60)
                     AATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTG                                                                         >  SRR4302547.1139161/1‑94 (MQ=60)
                      ATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGT                                                                        >  SRR4302547.627877/1‑94 (MQ=60)
                      ATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGT                                                                        >  SRR4302547.2303879/1‑94 (MQ=60)
                       TATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTT                                                                       <  SRR4302547.2412640/94‑1 (MQ=60)
                       TATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTT                                                                       <  SRR4302547.6092249/94‑1 (MQ=60)
                        ATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTT                                                                      >  SRR4302547.3735725/1‑94 (MQ=60)
                        ATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTT                                                                      <  SRR4302547.3253387/94‑1 (MQ=60)
                        ATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGACATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTT                                                                      >  SRR4302547.2427136/1‑94 (MQ=60)
                         TATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTA                                                                     >  SRR4302547.4170895/1‑94 (MQ=60)
                         TATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTA                                                                     >  SRR4302547.773068/1‑94 (MQ=60)
                          ATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAA                                                                    >  SRR4302547.4589394/1‑94 (MQ=60)
                          ATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAA                                                                    <  SRR4302547.4390622/94‑1 (MQ=60)
                           TTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAAT                                                                   <  SRR4302547.5592480/94‑1 (MQ=60)
                           TTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAAT                                                                   <  SRR4302547.668068/94‑1 (MQ=60)
                             GAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTG                                                                 <  SRR4302547.2486423/94‑1 (MQ=60)
                               ATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCC                                                               <  SRR4302547.167485/94‑1 (MQ=60)
                               ATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCC                                                               >  SRR4302547.4126356/1‑94 (MQ=60)
                               ATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCC                                                               <  SRR4302547.4786719/94‑1 (MQ=60)
                                  CGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGA                                                            >  SRR4302547.5049148/1‑94 (MQ=60)
                                   GCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAT                                                           >  SRR4302547.3233769/1‑94 (MQ=60)
                                   GCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAT                                                           <  SRR4302547.2524445/94‑1 (MQ=60)
                                   GCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAT                                                           >  SRR4302547.3213917/1‑94 (MQ=60)
                                   GCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCCAAATCTGTTTAATTGCCGGAT                                                           <  SRR4302547.5546124/94‑1 (MQ=60)
                                   GCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAT                                                           <  SRR4302547.920099/94‑1 (MQ=60)
                                    CAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATG                                                          <  SRR4302547.3230765/94‑1 (MQ=60)
                                    CAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATG                                                          <  SRR4302547.456330/94‑1 (MQ=60)
                                    CAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATG                                                          <  SRR4302547.4171314/94‑1 (MQ=60)
                                    CAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATG                                                          <  SRR4302547.6484060/94‑1 (MQ=60)
                                    CAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATG                                                          >  SRR4302547.581700/1‑94 (MQ=60)
                                    CAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCAGGCATGAACAAAGCAAGCTTTGCCAACAATCTAAATCTGTTTAATTGGCGGATG                                                          >  SRR4302547.841646/1‑94 (MQ=60)
                                     AAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGT                                                         >  SRR4302547.6642322/1‑94 (MQ=60)
                                      AGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTC                                                        >  SRR4302547.5762112/1‑94 (MQ=60)
                                       GGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCA                                                       >  SRR4302547.5758629/1‑94 (MQ=60)
                                        GATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAG                                                      <  SRR4302547.1433061/94‑1 (MQ=60)
                                        GATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAG                                                      >  SRR4302547.3329839/1‑94 (MQ=60)
                                        GATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAG                                                      <  SRR4302547.4811477/94‑1 (MQ=60)
                                         ATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGA                                                     >  SRR4302547.6610793/1‑94 (MQ=60)
                                          TTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGAC                                                    <  SRR4302547.1089397/94‑1 (MQ=60)
                                          TTTGTAGGCCGCATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGAC                                                    <  SRR4302547.2407799/94‑1 (MQ=60)
                                           TTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACA                                                   <  SRR4302547.4909439/94‑1 (MQ=60)
                                           TTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACA                                                   >  SRR4302547.539780/1‑94 (MQ=60)
                                           TTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACA                                                   >  SRR4302547.1634329/1‑94 (MQ=60)
                                           TTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACA                                                   >  SRR4302547.4474715/1‑94 (MQ=60)
                                            TGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACAT                                                  >  SRR4302547.2786290/1‑94 (MQ=60)
                                            TGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACAT                                                  >  SRR4302547.1048708/1‑94 (MQ=60)
                                            TGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACAT                                                  >  SRR4302547.6598725/1‑94 (MQ=60)
                                            TGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACAT                                                  >  SRR4302547.1669760/1‑94 (MQ=60)
                                              TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCC                                                <  SRR4302547.3625881/94‑1 (MQ=60)
                                              TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCC                                                >  SRR4302547.4728260/1‑94 (MQ=60)
                                              TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCC                                                <  SRR4302547.3387979/94‑1 (MQ=60)
                                              TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCC                                                <  SRR4302547.3500714/94‑1 (MQ=60)
                                              TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCC                                                >  SRR4302547.6010251/1‑94 (MQ=60)
                                               AGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTGAGACATCCG                                               >  SRR4302547.2177744/1‑94 (MQ=60)
                                               AGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCG                                               >  SRR4302547.1832484/1‑94 (MQ=60)
                                               AGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCG                                               >  SRR4302547.869189/1‑94 (MQ=60)
                                               AGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCCGATGACAGACATACG                                               >  SRR4302547.1287445/1‑94 (MQ=60)
                                                GGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGTCGGATGTCAGACATCCGG                                              >  SRR4302547.4278646/1‑94 (MQ=60)
                                                GGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGG                                              <  SRR4302547.3819891/94‑1 (MQ=60)
                                                GGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGG                                              >  SRR4302547.2235501/1‑94 (MQ=60)
                                                 GCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGC                                             >  SRR4302547.2022657/1‑94 (MQ=60)
                                                 GCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGC                                             <  SRR4302547.3308833/94‑1 (MQ=60)
                                                  CCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCA                                            >  SRR4302547.3420322/1‑94 (MQ=60)
                                                  CCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCA                                            <  SRR4302547.5652293/94‑1 (MQ=60)
                                                  CCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCA                                            <  SRR4302547.678952/94‑1 (MQ=60)
                                                   CGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAA                                           >  SRR4302547.4645546/1‑94 (MQ=60)
                                                   CGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAA                                           >  SRR4302547.5279135/1‑94 (MQ=60)
                                                    GGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAT                                          >  SRR4302547.4278585/1‑94 (MQ=60)
                                                    GGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAT                                          <  SRR4302547.1168718/94‑1 (MQ=60)
                                                    GGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAT                                          >  SRR4302547.1316384/1‑94 (MQ=60)
                                                    GGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAT                                          >  SRR4302547.6694758/1‑94 (MQ=60)
                                                     GATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATT                                         <  SRR4302547.105502/94‑1 (MQ=60)
                                                     GATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATT                                         <  SRR4302547.1310330/94‑1 (MQ=60)
                                                     GATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATT                                         >  SRR4302547.5134256/1‑94 (MQ=60)
                                                     GATAAGCCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATT                                         <  SRR4302547.5765628/94‑1 (MQ=60)
                                                      ATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATCA                                        >  SRR4302547.2474084/1‑94 (MQ=60)
                                                       TAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAT                                       <  SRR4302547.4893244/94‑1 (MQ=60)
                                                       TAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAT                                       <  SRR4302547.1297738/94‑1 (MQ=60)
                                                        AAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATC                                      >  SRR4302547.5413273/1‑94 (MQ=60)
                                                         AGGCGTTCCCCCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCA                                     <  SRR4302547.2421131/94‑1 (MQ=60)
                                                         AGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCA                                     <  SRR4302547.4940550/94‑1 (MQ=60)
                                                         AGGCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCA                                     >  SRR4302547.4751402/1‑94 (MQ=60)
                                                           GCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATC                                   <  SRR4302547.6500186/94‑1 (MQ=60)
                                                           GCGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATC                                   >  SRR4302547.2733297/1‑94 (MQ=60)
                                                            CGTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCA                                  <  SRR4302547.2850657/94‑1 (MQ=60)
                                                            CGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCA                                  >  SRR4302547.4836622/1‑94 (MQ=60)
                                                             TTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAC                                 >  SRR4302547.4281259/1‑94 (MQ=60)
                                                             GTTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAC                                 >  SRR4302547.6283038/1‑94 (MQ=60)
                                                              TTCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACT                                <  SRR4302547.2788475/94‑1 (MQ=60)
                                                               TCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATTACTT                               >  SRR4302547.3492765/1‑94 (MQ=60)
                                                               TCACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTT                               <  SRR4302547.3596643/94‑1 (MQ=60)
                                                                CACGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTC                              >  SRR4302547.5857144/1‑94 (MQ=60)
                                                                CACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTT                              >  SRR4302547.6192471/1‑94 (MQ=60)
                                                                  CGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTT                            <  SRR4302547.6413212/94‑1 (MQ=60)
                                                                  CGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTT                            <  SRR4302547.5793311/94‑1 (MQ=60)
                                                                  CGCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTT                            <  SRR4302547.2962505/94‑1 (MQ=60)
                                                                  CGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTT                            >  SRR4302547.6724259/1‑94 (MQ=60)
                                                                   GCCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATTACTTCTTC                           >  SRR4302547.2795459/1‑94 (MQ=60)
                                                                    CCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCA                          <  SRR4302547.1099521/94‑1 (MQ=60)
                                                                    CCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCA                          >  SRR4302547.847136/1‑94 (MQ=60)
                                                                    CCGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCA                          <  SRR4302547.707495/94‑1 (MQ=60)
                                                                    CCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCA                          >  SRR4302547.3978561/1‑94 (MQ=60)
                                                                    CCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCA                          <  SRR4302547.1812939/94‑1 (MQ=60)
                                                                    CCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCA                          <  SRR4302547.1995388/94‑1 (MQ=60)
                                                                     CGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAT                         >  SRR4302547.6293053/1‑94 (MQ=60)
                                                                     CGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAT                         >  SRR4302547.4107385/1‑94 (MQ=60)
                                                                     CGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAT                         <  SRR4302547.5754301/94‑1 (MQ=60)
                                                                     CGCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAT                         <  SRR4302547.5596405/94‑1 (MQ=60)
                                                                     CGCATCCGGCATGAACAAAGCACGCTTTACCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCAT                         <  SRR4302547.3063516/94‑1 (MQ=60)
                                                                      GCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATC                        >  SRR4302547.85038/1‑94 (MQ=60)
                                                                      GCATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATC                        <  SRR4302547.5661960/94‑1 (MQ=60)
                                                                       CATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCT                       >  SRR4302547.238819/1‑94 (MQ=60)
                                                                        ATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTG                      <  SRR4302547.256841/94‑1 (MQ=60)
                                                                        ATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTG                      <  SRR4302547.1792163/94‑1 (MQ=60)
                                                                        ATCCGGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTG                      >  SRR4302547.5887521/1‑94 (MQ=60)
                                                                           CGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGA                   >  SRR4302547.1554441/1‑94 (MQ=60)
                                                                            GGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGAC                  <  SRR4302547.2352131/94‑1 (MQ=60)
                                                                            GGCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGAC                  <  SRR4302547.1444332/94‑1 (MQ=60)
                                                                             GCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACA                 <  SRR4302547.1675377/94‑1 (MQ=60)
                                                                             GCATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACA                 <  SRR4302547.5468133/94‑1 (MQ=60)
                                                                             GCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACA                 >  SRR4302547.5320222/1‑94 (MQ=60)
                                                                             GCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACA                 >  SRR4302547.444936/1‑94 (MQ=60)
                                                                              CATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAA                >  SRR4302547.654950/1‑94 (MQ=60)
                                                                              CATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAA                >  SRR4302547.1436472/1‑94 (MQ=60)
                                                                              CATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAA                >  SRR4302547.2705555/1‑94 (MQ=60)
                                                                              CATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAA                <  SRR4302547.799554/94‑1 (MQ=60)
                                                                              CATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAA                <  SRR4302547.296722/94‑1 (MQ=60)
                                                                               ATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAA               <  SRR4302547.3596080/94‑1 (MQ=60)
                                                                               ATGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAA               >  SRR4302547.4621183/1‑94 (MQ=60)
                                                                                TGAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAA              <  SRR4302547.3682029/94‑1 (MQ=60)
                                                                                TGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAA              >  SRR4302547.5939150/1‑94 (MQ=60)
                                                                                 GAACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAAT             <  SRR4302547.598310/94‑1 (MQ=60)
                                                                                 GAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAAT             >  SRR4302547.4634329/1‑94 (MQ=60)
                                                                                  AACAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATC            <  SRR4302547.6036654/94‑1 (MQ=60)
                                                                                  AACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATC            <  SRR4302547.3016499/94‑1 (MQ=60)
                                                                                   ACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCG           <  SRR4302547.2241749/94‑1 (MQ=60)
                                                                                    CAAAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGT          <  SRR4302547.5338705/94‑1 (MQ=60)
                                                                                      AAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCC        >  SRR4302547.4885523/1‑94 (MQ=60)
                                                                                      AAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCC        <  SRR4302547.255735/94‑1 (MQ=60)
                                                                                      AAGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCC        >  SRR4302547.2186193/1‑94 (MQ=60)
                                                                                      AAACACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCCGATGACAGACATCCGACAATTATCATCACTTCTTCTTCTGCGACAAAATCGTCC        >  SRR4302547.329461/1‑94 (MQ=60)
                                                                                       AGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCG       <  SRR4302547.645325/94‑1 (MQ=60)
                                                                                       AGCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCG       <  SRR4302547.501773/94‑1 (MQ=60)
                                                                                        GCACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGA      <  SRR4302547.6128316/94‑1 (MQ=60)
                                                                                        GCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGA      <  SRR4302547.4592584/94‑1 (MQ=60)
                                                                                         CACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGAC     >  SRR4302547.5640463/1‑94 (MQ=60)
                                                                                         CACGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGAC     >  SRR4302547.263976/1‑94 (MQ=60)
                                                                                         CACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGAC     >  SRR4302547.905961/1‑94 (MQ=60)
                                                                                          ACGCTTTGCCAACAATCTAAATCTGGTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACA    >  SRR4302547.650147/1‑94 (MQ=60)
                                                                                           CGCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACAC   >  SRR4302547.2460471/1‑94 (MQ=60)
                                                                                            GCTTTGCCAACAGTCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACT  >  SRR4302547.1169859/1‑94 (MQ=60)
                                                                                            GCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACT  >  SRR4302547.1311001/1‑94 (MQ=60)
                                                                                            GCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACT  <  SRR4302547.701685/94‑1 (MQ=60)
                                                                                            GCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACT  <  SRR4302547.1639283/94‑1 (MQ=60)
                                                                                            GCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACT  <  SRR4302547.6097677/94‑1 (MQ=60)
                                                                                            |                                                                                             
ATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACT  >  NC_000913/3673769‑3673954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑