Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,736,298 T→C N391N (AAT→AAC)  malS → alpha‑amylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,2980TC100.0% 999.5 / NA 271N391N (AAT→AAC) malSalpha‑amylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (149/122);  total (149/122)

Only 100 of 284 total aligned reads displayed.

TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTA  >  NC_000913/3736208‑3736387
                                                                                          |                                                                                         
tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         >  2:909723/1‑93 (MQ=255)
tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         >  2:4083729/1‑93 (MQ=255)
 aaCGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCt                                                                                        >  1:5551505/1‑93 (MQ=255)
 aaCGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCt                                                                                        >  1:3690814/1‑93 (MQ=255)
    gATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGa                                                                                     >  1:1015258/1‑93 (MQ=255)
    gATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGa                                                                                     <  1:2307178/93‑1 (MQ=255)
     aTTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGAt                                                                                    >  2:4390427/1‑93 (MQ=255)
      ttACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGAtt                                                                                   <  2:6381722/93‑1 (MQ=255)
      ttACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGAtt                                                                                   >  2:4746118/1‑93 (MQ=255)
       tACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTc                                                                                  >  2:5793291/1‑93 (MQ=255)
       tACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTc                                                                                  <  2:1990139/93‑1 (MQ=255)
          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgac                                                                               <  2:6223107/93‑1 (MQ=255)
          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgac                                                                               <  2:5150400/93‑1 (MQ=255)
          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgac                                                                               <  2:3418329/93‑1 (MQ=255)
          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgac                                                                               >  2:2461961/1‑93 (MQ=255)
            tAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgacga                                                                             >  1:1129645/1‑93 (MQ=255)
            tAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgacga                                                                             <  1:2805187/93‑1 (MQ=255)
             aaTTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAt                                                                            <  1:4827501/93‑1 (MQ=255)
             aaTTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAt                                                                            >  1:6034628/1‑93 (MQ=255)
              aTTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAtc                                                                           >  1:6508853/1‑93 (MQ=255)
              aTTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAtc                                                                           <  2:5533247/93‑1 (MQ=255)
               tttCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAtct                                                                          <  1:2389054/93‑1 (MQ=255)
                  cAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCAc                                                                       <  1:315487/93‑1 (MQ=255)
                   aGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACt                                                                      <  1:5895787/93‑1 (MQ=255)
                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     <  1:790912/93‑1 (MQ=255)
                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     >  2:5584125/1‑93 (MQ=255)
                      gACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATg                                                                   >  2:5425544/1‑93 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  1:4140233/1‑93 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  1:5963877/1‑93 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  2:5373201/1‑93 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  <  1:701029/93‑1 (MQ=255)
                        aaaaaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTc                                                                 <  1:3564960/92‑1 (MQ=255)
                         aaaaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCg                                                                >  2:5061753/1‑93 (MQ=255)
                          aaaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGc                                                               >  2:6562973/1‑93 (MQ=255)
                          aaaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGc                                                               >  1:2518788/1‑93 (MQ=255)
                           aaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCt                                                              <  2:1309383/93‑1 (MQ=255)
                           aaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCt                                                              >  1:626654/1‑93 (MQ=255)
                            aCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTa                                                             <  1:2587936/93‑1 (MQ=255)
                            aCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTa                                                             <  2:6528191/93‑1 (MQ=255)
                             cAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAg                                                            <  2:5295251/93‑1 (MQ=255)
                                 cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtt                                                        <  1:272199/93‑1 (MQ=255)
                                   gggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtttt                                                      <  1:154296/93‑1 (MQ=255)
                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  1:5986970/93‑1 (MQ=255)
                                      aTAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGc                                                   <  1:5496661/93‑1 (MQ=255)
                                         aaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCgg                                                <  1:2070405/93‑1 (MQ=255)
                                          aTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGa                                               >  1:1212496/1‑93 (MQ=255)
                                           tggtggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGat                                              >  1:4426305/1‑93 (MQ=255)
                                            ggtggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGata                                             <  2:2273020/93‑1 (MQ=255)
                                            ggtggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATATCACTATGTCGCTAGCCTTTTTGCCGGata                                             <  2:3970348/93‑1 (MQ=255)
                                             gtggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGatat                                            <  2:4871482/93‑1 (MQ=255)
                                               ggggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCa                                          >  1:4388602/1‑93 (MQ=255)
                                               ggggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCa                                          >  1:4728495/1‑93 (MQ=255)
                                               ggggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCa                                          <  2:1415892/93‑1 (MQ=255)
                                                 ggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaa                                        >  2:1134288/1‑93 (MQ=255)
                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCCCTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       >  2:743531/1‑93 (MQ=255)
                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       <  1:1482748/93‑1 (MQ=255)
                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       <  2:5996039/93‑1 (MQ=255)
                                                    aaaaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAcc                                     >  2:336338/1‑93 (MQ=255)
                                                    aaaaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAcc                                     >  1:5195746/1‑93 (MQ=255)
                                                     aaaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCg                                    >  1:1401255/1‑93 (MQ=255)
                                                     aaaaCTGGATCAGAACGGATATCGGCGATAACGACACCCCGGAATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCg                                    <  1:793696/93‑1 (MQ=255)
                                                       aaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGaa                                  >  1:3055310/1‑93 (MQ=255)
                                                         cTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATc                                <  1:6596455/93‑1 (MQ=255)
                                                          tGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCa                               >  1:5457166/1‑93 (MQ=255)
                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              >  1:609931/1‑93 (MQ=255)
                                                            gATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAc                             >  2:5003182/1‑93 (MQ=255)
                                                            gATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAc                             >  1:3863783/1‑93 (MQ=255)
                                                            gATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAc                             >  2:2403889/1‑93 (MQ=255)
                                                            gATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAc                             >  1:1615098/1‑93 (MQ=255)
                                                            gATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAc                             <  2:1001571/93‑1 (MQ=255)
                                                              tCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTa                           <  2:2484972/93‑1 (MQ=255)
                                                               cAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTAc                          <  2:5977238/93‑1 (MQ=255)
                                                                  aaCGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGc                       <  1:1644303/93‑1 (MQ=255)
                                                                   aCGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCt                      <  1:4705831/93‑1 (MQ=255)
                                                                   aCGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCt                      >  1:3386378/1‑93 (MQ=255)
                                                                    cGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCtt                     >  2:1009846/1‑93 (MQ=255)
                                                                    cGGATATCGGCGATTACGACAACCCTCGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCtt                     <  2:3199489/93‑1 (MQ=255)
                                                                     ggATATCGGCGATTACGACACCCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTc                    <  1:571348/93‑1 (MQ=255)
                                                                     ggATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTc                    >  2:3496112/1‑93 (MQ=255)
                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   >  1:1924018/1‑93 (MQ=255)
                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   <  2:265495/93‑1 (MQ=255)
                                                                        tatCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTgg                 >  2:3915274/1‑93 (MQ=255)
                                                                        tatCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTgg                 <  1:4513500/93‑1 (MQ=255)
                                                                           cGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCt              >  2:148309/1‑93 (MQ=255)
                                                                              cGATTACGACACCCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAACCCGAATCAACTACCGCTTCTGGTCTGcc           <  1:5018667/93‑1 (MQ=255)
                                                                              cGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGcc           >  2:5697746/1‑93 (MQ=255)
                                                                               gATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCg          >  2:1696165/1‑93 (MQ=255)
                                                                               gATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCg          >  1:3012105/1‑93 (MQ=255)
                                                                                aTTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCgg         <  1:2958421/93‑1 (MQ=255)
                                                                                aTTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCgg         <  1:4789866/93‑1 (MQ=255)
                                                                                 ttACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgt        <  1:5793291/93‑1 (MQ=255)
                                                                                   aCGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt      <  2:4629532/93‑1 (MQ=255)
                                                                                   aCGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt      <  2:4482692/93‑1 (MQ=255)
                                                                                   aCGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt      >  2:2114584/1‑93 (MQ=255)
                                                                                    cGACAACCCTGCATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGtt     <  2:3021213/93‑1 (MQ=255)
                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGGTATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    >  2:6099212/1‑93 (MQ=255)
                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    <  1:4315370/93‑1 (MQ=255)
                                                                                      aCAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCt   >  2:4167117/1‑93 (MQ=255)
                                                                                      aCAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCt   >  1:5607983/1‑93 (MQ=255)
                                                                                       cAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCta  >  2:2827276/1‑93 (MQ=255)
                                                                                          |                                                                                         
TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTA  >  NC_000913/3736208‑3736387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAA  >  NC_000913/3736205‑3736391
                                                                                             |                                                                                             
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                               >  SRR4302547.5089879/1‑94 (MQ=60)
 TTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACC                                                                                              >  SRR4302547.3591442/1‑94 (MQ=60)
 TTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACC                                                                                              <  SRR4302547.1584898/94‑1 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                            >  SRR4302547.3766163/1‑94 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                            <  SRR4302547.4495135/94‑1 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                            >  SRR4302547.5666041/1‑94 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                            <  SRR4302547.6139861/94‑1 (MQ=60)
    AGCGATTACATTAATTTCAGCGACAAAACAGGCTGGGAGAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTG                                                                                           >  SRR4302547.203995/1‑94 (MQ=60)
    AACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTG                                                                                           >  SRR4302547.3298032/1‑94 (MQ=60)
    AACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTG                                                                                           >  SRR4302547.3864644/1‑94 (MQ=60)
     ACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGG                                                                                          <  SRR4302547.1401216/94‑1 (MQ=60)
      CGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGA                                                                                         >  SRR4302547.1767223/1‑94 (MQ=60)
      CGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGA                                                                                         >  SRR4302547.1035138/1‑94 (MQ=60)
       GATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGAT                                                                                        <  SRR4302547.2353944/94‑1 (MQ=60)
        ATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATT                                                                                       <  SRR4302547.563497/94‑1 (MQ=60)
        ATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATT                                                                                       >  SRR4302547.4261014/1‑94 (MQ=60)
         TTACCTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTC                                                                                      <  SRR4302547.1489149/94‑1 (MQ=60)
         TTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTC                                                                                      <  SRR4302547.788804/94‑1 (MQ=60)
         TTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTC                                                                                      <  SRR4302547.5941621/94‑1 (MQ=60)
         TTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTC                                                                                      >  SRR4302547.4597414/1‑94 (MQ=60)
         TTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTC                                                                                      >  SRR4302547.556318/1‑94 (MQ=60)
         TTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTC                                                                                      <  SRR4302547.1219417/94‑1 (MQ=60)
          TACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCG                                                                                     <  SRR4302547.6651977/94‑1 (MQ=60)
           ACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGA                                                                                    <  SRR4302547.2772746/94‑1 (MQ=60)
             ATTAATTTCAGCGACAAAACAGCCTGGGATAAAGGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACG                                                                                  <  SRR4302547.4783642/94‑1 (MQ=60)
              TTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGA                                                                                 >  SRR4302547.1152448/1‑94 (MQ=60)
               TAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAT                                                                                >  SRR4302547.6159857/1‑94 (MQ=60)
               TAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAT                                                                                <  SRR4302547.2861338/94‑1 (MQ=60)
               TAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAT                                                                                <  SRR4302547.2383608/94‑1 (MQ=60)
                AATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATC                                                                               >  SRR4302547.6094741/1‑94 (MQ=60)
                AATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATC                                                                               <  SRR4302547.4927591/94‑1 (MQ=60)
                AATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATC                                                                               >  SRR4302547.6644374/1‑94 (MQ=60)
                 ATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCT                                                                              <  SRR4302547.1594553/94‑1 (MQ=60)
                 ATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCT                                                                              <  SRR4302547.713919/94‑1 (MQ=60)
                 AGTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCT                                                                              <  SRR4302547.1135247/94‑1 (MQ=60)
                  TTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTC                                                                             <  SRR4302547.2437452/94‑1 (MQ=60)
                  TTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTC                                                                             >  SRR4302547.258933/1‑94 (MQ=60)
                    TCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCAC                                                                           >  SRR4302547.3102663/1‑94 (MQ=60)
                     CAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACT                                                                          <  SRR4302547.321363/94‑1 (MQ=60)
                     CAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACT                                                                          >  SRR4302547.5910322/1‑94 (MQ=60)
                      AGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTA                                                                         >  SRR4302547.4806291/1‑94 (MQ=60)
                      AGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTA                                                                         <  SRR4302547.6017870/94‑1 (MQ=60)
                       GCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTAT                                                                        <  SRR4302547.805882/94‑1 (MQ=60)
                       GCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTAT                                                                        <  SRR4302547.3133825/94‑1 (MQ=60)
                       GCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTAT                                                                        <  SRR4302547.1543608/94‑1 (MQ=60)
                        CGACAAAACAGGCTGGGATAAATGGGGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATG                                                                       >  SRR4302547.2310818/1‑94 (MQ=60)
                         GACAAAACAGGCTGGGGGAAATGGTGGGGAATAAACTGGCTCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGAAGATCTCACTATGC                                                                      >  SRR4302547.5381990/1‑94 (MQ=60)
                         GACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGT                                                                      >  SRR4302547.4224484/1‑94 (MQ=60)
                         GACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGT                                                                      >  SRR4302547.6087563/1‑94 (MQ=60)
                         GACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGT                                                                      >  SRR4302547.2406587/1‑94 (MQ=60)
                          ACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTC                                                                     <  SRR4302547.713985/94‑1 (MQ=60)
                           AAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCG                                                                    <  SRR4302547.3637704/94‑1 (MQ=60)
                            AAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGC                                                                   >  SRR4302547.2569701/1‑94 (MQ=60)
                             AAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCT                                                                  >  SRR4302547.638223/1‑94 (MQ=60)
                              AACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTA                                                                 >  SRR4302547.3707980/1‑94 (MQ=60)
                               ACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGTTTCGACGATCTAACTATTTCGCTAG                                                                >  SRR4302547.5672980/1‑94 (MQ=60)
                               ACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAG                                                                <  SRR4302547.2640149/94‑1 (MQ=60)
                                CAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGC                                                               >  SRR4302547.2820626/1‑94 (MQ=60)
                                 AGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCC                                                              <  SRR4302547.6059510/94‑1 (MQ=60)
                                  GGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCT                                                             <  SRR4302547.4407555/94‑1 (MQ=60)
                                    CTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTT                                                           >  SRR4302547.2737754/1‑94 (MQ=60)
                                    CTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTT                                                           >  SRR4302547.1248225/1‑94 (MQ=60)
                                    CTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTT                                                           <  SRR4302547.277291/94‑1 (MQ=60)
                                    CTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTT                                                           <  SRR4302547.5760779/94‑1 (MQ=60)
                                     TGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTT                                                          >  SRR4302547.6091436/1‑94 (MQ=60)
                                      GGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTT                                                         <  SRR4302547.3855711/94‑1 (MQ=60)
                                      GGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTT                                                         >  SRR4302547.3545798/1‑94 (MQ=60)
                                      GGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTT                                                         <  SRR4302547.157115/94‑1 (MQ=60)
                                       GGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTG                                                        <  SRR4302547.6111176/94‑1 (MQ=60)
                                       GGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTG                                                        <  SRR4302547.4901756/94‑1 (MQ=60)
                                        GGTAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGC                                                       <  SRR4302547.151987/94‑1 (MQ=60)
                                         ATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCC                                                      <  SRR4302547.5610150/94‑1 (MQ=60)
                                         ATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCC                                                      <  SRR4302547.424200/94‑1 (MQ=60)
                                          TAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCG                                                     <  SRR4302547.1486432/94‑1 (MQ=60)
                                          TAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCG                                                     <  SRR4302547.5914807/94‑1 (MQ=60)
                                          TAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCG                                                     >  SRR4302547.5876965/1‑94 (MQ=60)
                                            AATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGA                                                   >  SRR4302547.1236968/1‑94 (MQ=60)
                                            AATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGA                                                   >  SRR4302547.2956673/1‑94 (MQ=60)
                                            AATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGA                                                   >  SRR4302547.4485016/1‑94 (MQ=60)
                                            AATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGA                                                   <  SRR4302547.2111732/94‑1 (MQ=60)
                                            AATGGTGGGGAAAAAACTGGATCAGAACGGATAGCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGA                                                   >  SRR4302547.806766/1‑94 (MQ=60)
                                             ATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGAT                                                  >  SRR4302547.4517768/1‑94 (MQ=60)
                                             ATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGAT                                                  >  SRR4302547.4858943/1‑94 (MQ=60)
                                              TGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATA                                                 <  SRR4302547.6695207/94‑1 (MQ=60)
                                              TGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATA                                                 <  SRR4302547.4204263/94‑1 (MQ=60)
                                               GGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATAT                                                <  SRR4302547.558925/94‑1 (MQ=60)
                                                 TGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCA                                              >  SRR4302547.4478893/1‑94 (MQ=60)
                                                 TGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCA                                              >  SRR4302547.4826483/1‑94 (MQ=60)
                                                 GGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCA                                              <  SRR4302547.5215471/94‑1 (MQ=60)
                                                  GGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAA                                             >  SRR4302547.3081918/1‑94 (MQ=60)
                                                   GGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAA                                            >  SRR4302547.2451384/1‑94 (MQ=60)
                                                    GGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAA                                           <  SRR4302547.6038298/94‑1 (MQ=60)
                                                     GAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAC                                          <  SRR4302547.1512339/94‑1 (MQ=60)
                                                     GAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAC                                          <  SRR4302547.3614342/94‑1 (MQ=60)
                                                      AAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACC                                         >  SRR4302547.5302675/1‑94 (MQ=60)
                                                       AAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCG                                        >  SRR4302547.1429290/1‑94 (MQ=60)
                                                        AAAACTGGATCAGAACGGATATCGGCGATAACGACACCCCGGAATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGA                                       <  SRR4302547.808712/94‑1 (MQ=60)
                                                         AAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAA                                      >  SRR4302547.83664/1‑94 (MQ=60)
                                                         AAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAA                                      >  SRR4302547.6271149/1‑94 (MQ=60)
                                                         AAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAA                                      >  SRR4302547.971915/1‑94 (MQ=60)
                                                         AAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAA                                      >  SRR4302547.3116110/1‑94 (MQ=60)
                                                          AACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAT                                     >  SRR4302547.5257545/1‑94 (MQ=60)
                                                           ACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATC                                    >  SRR4302547.2813332/1‑94 (MQ=60)
                                                            CTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA                                   <  SRR4302547.6733784/94‑1 (MQ=60)
                                                            CTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA                                   >  SRR4302547.6255503/1‑94 (MQ=60)
                                                            CTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA                                   <  SRR4302547.721326/94‑1 (MQ=60)
                                                            CTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA                                   >  SRR4302547.5569919/1‑94 (MQ=60)
                                                            CTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA                                   <  SRR4302547.5728524/94‑1 (MQ=60)
                                                             TGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAA                                  >  SRR4302547.621264/1‑94 (MQ=60)
                                                              GGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAC                                 >  SRR4302547.3699920/1‑94 (MQ=60)
                                                              GGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAC                                 >  SRR4302547.3942401/1‑94 (MQ=60)
                                                              GGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAC                                 >  SRR4302547.1647073/1‑94 (MQ=60)
                                                               GATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACT                                >  SRR4302547.4579096/1‑94 (MQ=60)
                                                               GATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGCATATCAAAACCGAATCAACT                                <  SRR4302547.6639262/94‑1 (MQ=60)
                                                                  CAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACC                             <  SRR4302547.2546066/94‑1 (MQ=60)
                                                                    GAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGC                           >  SRR4302547.620556/1‑94 (MQ=60)
                                                                    GAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGC                           >  SRR4302547.6542228/1‑94 (MQ=60)
                                                                     AACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCACAACCGAATCAACTACCGCT                          >  SRR4302547.6189703/1‑94 (MQ=60)
                                                                     AACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCT                          >  SRR4302547.4823841/1‑94 (MQ=60)
                                                                     AACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCT                          <  SRR4302547.1676790/94‑1 (MQ=60)
                                                                     AACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCT                          >  SRR4302547.3070580/1‑94 (MQ=60)
                                                                     AACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCT                          >  SRR4302547.3455255/1‑94 (MQ=60)
                                                                      ACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTT                         <  SRR4302547.4803280/94‑1 (MQ=60)
                                                                        GGATATCGGCGATTACGACACCCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCT                       <  SRR4302547.581617/94‑1 (MQ=60)
                                                                        GGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCT                       >  SRR4302547.1962131/1‑94 (MQ=60)
                                                                         GATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTG                      >  SRR4302547.5532945/1‑94 (MQ=60)
                                                                         GATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTG                      >  SRR4302547.1612131/1‑94 (MQ=60)
                                                                         GATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTG                      >  SRR4302547.5634722/1‑94 (MQ=60)
                                                                          ATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGG                     >  SRR4302547.4973121/1‑94 (MQ=60)
                                                                           TATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGT                    <  SRR4302547.3038359/94‑1 (MQ=60)
                                                                           TATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGT                    >  SRR4302547.1673768/1‑94 (MQ=60)
                                                                           TATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGT                    <  SRR4302547.4606756/94‑1 (MQ=60)
                                                                           TATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACAGCTTCTGGT                    >  SRR4302547.2331186/1‑94 (MQ=60)
                                                                            ATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTC                   >  SRR4302547.4885452/1‑94 (MQ=60)
                                                                             TCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCT                  <  SRR4302547.1801318/94‑1 (MQ=60)
                                                                              CGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTG                 >  SRR4302547.5438085/1‑94 (MQ=60)
                                                                              CGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTG                 <  SRR4302547.687364/94‑1 (MQ=60)
                                                                                 CGATTACGACACCCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAACCCGAATCAACTACCGCTTCTGGTCTGCCG              <  SRR4302547.5122384/94‑1 (MQ=60)
                                                                                 CGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCG              >  SRR4302547.3071988/1‑94 (MQ=60)
                                                                                 CGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCG              >  SRR4302547.1969905/1‑94 (MQ=60)
                                                                                  GATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGG             >  SRR4302547.2984686/1‑94 (MQ=60)
                                                                                  GATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGG             >  SRR4302547.3978403/1‑94 (MQ=60)
                                                                                  GATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGG             >  SRR4302547.4710421/1‑94 (MQ=60)
                                                                                   ATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGT            <  SRR4302547.3017302/94‑1 (MQ=60)
                                                                                   ATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGT            <  SRR4302547.4889104/94‑1 (MQ=60)
                                                                                    TTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTG           <  SRR4302547.5913128/94‑1 (MQ=60)
                                                                                      ACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTT         >  SRR4302547.5110194/1‑94 (MQ=60)
                                                                                      ACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTT         <  SRR4302547.1206522/94‑1 (MQ=60)
                                                                                        GACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCT       <  SRR4302547.3448196/94‑1 (MQ=60)
                                                                                        GACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCT       <  SRR4302547.4404179/94‑1 (MQ=60)
                                                                                        GACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCT       >  SRR4302547.5723701/1‑94 (MQ=60)
                                                                                           AACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATA    >  SRR4302547.4759427/1‑94 (MQ=60)
                                                                                            ACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAA   >  SRR4302547.3313234/1‑94 (MQ=60)
                                                                                            ACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAA   <  SRR4302547.4480771/94‑1 (MQ=60)
                                                                                             CCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAA  >  SRR4302547.5235781/1‑94 (MQ=60)
                                                                                             CCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAA  >  SRR4302547.1594008/1‑94 (MQ=60)
                                                                                             CCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAA  <  SRR4302547.5001242/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAA  >  NC_000913/3736205‑3736391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑