Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,736,298 T→C N391N (AAT→AAC)  malS → alpha‑amylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,2980TC99.6% 884.2 / ‑5.0 249N391N (AAT→AAC) malSalpha‑amylase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (129/119);  total (130/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

Only 100 of 261 total aligned reads displayed.

TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTA  >  NC_000913/3736208‑3736387
                                                                                          |                                                                                         
tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         <  1:1233900/93‑1 (MQ=255)
tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         >  1:299575/1‑93 (MQ=255)
tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         <  1:273377/93‑1 (MQ=255)
tAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAccc                                                                                         <  2:3538323/93‑1 (MQ=255)
 aaCGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCt                                                                                        >  1:2519572/1‑93 (MQ=255)
 aaCGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCt                                                                                        <  2:2965516/93‑1 (MQ=255)
   cGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTgg                                                                                      >  2:1457226/1‑93 (MQ=255)
    gATTACATTAATGTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGa                                                                                     <  1:5788373/93‑1 (MQ=255)
     aTTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGAt                                                                                    >  2:2307141/1‑93 (MQ=255)
       tACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTc                                                                                  <  1:3567412/93‑1 (MQ=255)
       tACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTc                                                                                  <  1:529430/93‑1 (MQ=255)
        aCATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcg                                                                                 >  2:4613646/1‑93 (MQ=255)
        aCATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcg                                                                                 >  2:4924736/1‑93 (MQ=255)
        aCATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcg                                                                                 >  1:5187576/1‑93 (MQ=255)
         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTCCGACAACCCTGGATTcga                                                                                <  1:655211/93‑1 (MQ=255)
         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcga                                                                                >  2:5065629/1‑93 (MQ=255)
         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcga                                                                                >  2:779372/1‑93 (MQ=255)
         cATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcga                                                                                >  1:621400/1‑93 (MQ=255)
          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgag                                                                               >  1:3100675/1‑92 (MQ=255)
          aTTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgac                                                                               >  1:1022140/1‑93 (MQ=255)
            tAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTcgacga                                                                             <  2:5118088/93‑1 (MQ=255)
               tttCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGAtcc                                                                          >  2:298874/1‑92 (MQ=255)
                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     <  1:6073244/93‑1 (MQ=255)
                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     <  1:4613689/93‑1 (MQ=255)
                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     >  2:2062429/1‑93 (MQ=255)
                    gCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTa                                                                     <  2:5378982/93‑1 (MQ=255)
                      gACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATg                                                                   <  1:2014239/93‑1 (MQ=255)
                      gACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATg                                                                   >  1:1124785/1‑93 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  1:968337/1‑93 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  2:1165945/1‑93 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  <  2:1139316/93‑1 (MQ=255)
                       aCAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGt                                                                  >  1:4047433/1‑93 (MQ=255)
                          aaaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGc                                                               <  2:4157432/93‑1 (MQ=255)
                           aaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCt                                                              >  2:3605561/1‑93 (MQ=255)
                           aaCAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCt                                                              >  2:4401796/1‑93 (MQ=255)
                            aCAGGCTGGGATAAATGGTGGGGAAAAAACTTGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTa                                                             <  1:1831691/93‑1 (MQ=255)
                              aGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGc                                                           >  2:3656801/1‑93 (MQ=255)
                               ggCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGcc                                                          <  1:4716909/93‑1 (MQ=255)
                                 cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGATGATCTCACTATGTCGCTAGCCtt                                                        >  2:1200760/1‑93 (MQ=255)
                                 cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtt                                                        >  2:712361/1‑93 (MQ=255)
                                 cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCAATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtt                                                        <  1:5762704/93‑1 (MQ=255)
                                   gggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCtttt                                                      >  2:5824550/1‑93 (MQ=255)
                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  1:2845829/93‑1 (MQ=255)
                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  1:4817756/93‑1 (MQ=255)
                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  2:2893612/93‑1 (MQ=255)
                                    ggATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCttttt                                                     <  2:3446266/93‑1 (MQ=255)
                                      aTAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGc                                                   >  1:37200/1‑93 (MQ=255)
                                       tAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGcc                                                  >  2:4463766/1‑93 (MQ=255)
                                        aaaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCg                                                 >  2:571974/1‑93 (MQ=255)
                                         aaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCgg                                                <  2:4689309/93‑1 (MQ=255)
                                         aaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCgg                                                >  2:550691/1‑93 (MQ=255)
                                         aaTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTCCCgg                                                >  1:3289201/1‑93 (MQ=255)
                                              tggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATc                                           >  2:2838651/1‑93 (MQ=255)
                                               ggggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCa                                          <  2:5651866/93‑1 (MQ=255)
                                                gggAAAAAACTGGATCAGAACGGATATCGTCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaa                                         <  1:3022123/93‑1 (MQ=255)
                                                gggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaa                                         <  1:1657441/93‑1 (MQ=255)
                                                gggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaa                                         <  1:4267190/93‑1 (MQ=255)
                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       >  2:3489354/1‑93 (MQ=255)
                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       <  2:4041466/93‑1 (MQ=255)
                                                  gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCaaaa                                       >  2:4376178/1‑93 (MQ=255)
                                                   aaaaaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAc                                      >  1:2401449/1‑93 (MQ=255)
                                                    aaaaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAcc                                     >  1:549907/1‑93 (MQ=255)
                                                      aaaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGa                                   <  2:25808/93‑1 (MQ=255)
                                                       aaCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGaa                                  <  1:166829/93‑1 (MQ=255)
                                                        aCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAt                                 >  1:5052817/1‑93 (MQ=255)
                                                        aCTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAt                                 <  2:3141267/93‑1 (MQ=255)
                                                         cTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATc                                >  1:670311/1‑93 (MQ=255)
                                                          tGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCa                               >  1:2141128/1‑93 (MQ=255)
                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              >  1:499097/1‑93 (MQ=255)
                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              <  1:2762560/93‑1 (MQ=255)
                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              >  1:3333365/1‑93 (MQ=255)
                                                           ggATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCaa                              <  2:3243125/93‑1 (MQ=255)
                                                            gATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAc                             >  2:6048764/1‑93 (MQ=255)
                                                             aTCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCACACCCGCATCAACt                            >  2:876415/1‑93 (MQ=255)
                                                             aTCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACt                            <  1:4312760/93‑1 (MQ=255)
                                                                 gAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCg                        <  2:1732202/93‑1 (MQ=255)
                                                                     ggATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCttt                    >  2:2204134/1‑92 (MQ=255)
                                                                     ggATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTc                    <  2:3679868/93‑1 (MQ=255)
                                                                     ggATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTc                    >  1:1691763/1‑93 (MQ=255)
                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   >  1:1910245/1‑93 (MQ=255)
                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   >  1:4118107/1‑93 (MQ=255)
                                                                      gATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCt                   <  2:1317888/93‑1 (MQ=255)
                                                                        tatCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTgg                 >  1:567295/1‑93 (MQ=255)
                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               >  2:2118563/1‑93 (MQ=255)
                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               <  1:1299532/93‑1 (MQ=255)
                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               >  2:2007658/1‑93 (MQ=255)
                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               >  1:806467/1‑93 (MQ=255)
                                                                          tCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTc               <  1:5897239/93‑1 (MQ=255)
                                                                            ggCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTg             >  1:4338647/1‑93 (MQ=255)
                                                                             gCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGc            <  2:1776548/93‑1 (MQ=255)
                                                                              cGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGcc           <  2:245319/93‑1 (MQ=255)
                                                                              cGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGcc           <  1:3835623/93‑1 (MQ=255)
                                                                               gATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCg          >  2:4841318/1‑93 (MQ=255)
                                                                                aTTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCgg         >  2:3833016/1‑93 (MQ=255)
                                                                                  tACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtg       <  2:1552101/93‑1 (MQ=255)
                                                                                   aCGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt      <  1:379318/93‑1 (MQ=255)
                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    <  1:143575/93‑1 (MQ=255)
                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    >  2:4331612/1‑93 (MQ=255)
                                                                                     gACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTc    <  2:4227614/93‑1 (MQ=255)
                                                                                       cAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCta  <  1:3436683/93‑1 (MQ=255)
                                                                                          |                                                                                         
TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTA  >  NC_000913/3736208‑3736387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTAT  >  NC_000913/3736205‑3736388
                                                                                             |                                                                                          
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                            <  SRR4302532.1858622/94‑1 (MQ=60)
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                            >  SRR4302532.2450510/1‑94 (MQ=60)
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                            >  SRR4302532.5754078/1‑94 (MQ=60)
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                            >  SRR4302532.1668002/1‑94 (MQ=60)
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                            >  SRR4302532.1668001/1‑94 (MQ=60)
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                            >  SRR4302532.5378145/1‑94 (MQ=60)
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAACGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAAC                                                                                            >  SRR4302532.1430263/1‑94 (MQ=60)
 TTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACC                                                                                           >  SRR4302532.5821274/1‑94 (MQ=60)
  TTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCC                                                                                          >  SRR4302532.309912/1‑94 (MQ=60)
  TTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGCATAAATGGTGGGTAAAAAACTGGATCAGACCGCATATCGGCGATTACGACAACCC                                                                                          <  SRR4302532.1022126/94‑1 (MQ=60)
  TTAACGATTACATTAATTGCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCC                                                                                          <  SRR4302532.2594353/94‑1 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                         <  SRR4302532.1386356/94‑1 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                         >  SRR4302532.2603642/1‑94 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                         <  SRR4302532.282846/94‑1 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                         <  SRR4302532.1275181/94‑1 (MQ=60)
   TAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCT                                                                                         <  SRR4302532.1230587/94‑1 (MQ=60)
    AACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTG                                                                                        >  SRR4302532.5956225/1‑94 (MQ=60)
     ACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGG                                                                                       <  SRR4302532.2841372/94‑1 (MQ=60)
     ACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGG                                                                                       <  SRR4302532.1730371/94‑1 (MQ=60)
      CGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGA                                                                                      <  SRR4302532.66781/94‑1 (MQ=60)
       GATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGTGATTACGACAACCCTGGAT                                                                                     >  SRR4302532.3853219/1‑94 (MQ=60)
       GATTACATTAATGTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGAT                                                                                     <  SRR4302532.5989636/94‑1 (MQ=60)
        ATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATT                                                                                    >  SRR4302532.546224/1‑94 (MQ=60)
        ATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATT                                                                                    >  SRR4302532.4234076/1‑94 (MQ=60)
         TTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTC                                                                                   >  SRR4302532.4420998/1‑94 (MQ=60)
          TACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCG                                                                                  <  SRR4302532.3686787/94‑1 (MQ=60)
          TACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCG                                                                                  <  SRR4302532.2493040/94‑1 (MQ=60)
          TACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCG                                                                                  >  SRR4302532.3626732/1‑94 (MQ=60)
          TACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCG                                                                                  <  SRR4302532.547912/94‑1 (MQ=60)
          TACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCG                                                                                  >  SRR4302532.5366336/1‑94 (MQ=60)
          TACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCG                                                                                  >  SRR4302532.2003899/1‑94 (MQ=60)
           ACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGA                                                                                 >  SRR4302532.643081/1‑94 (MQ=60)
           ACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGA                                                                                 >  SRR4302532.643082/1‑94 (MQ=60)
            CATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTCCGACAACCCTGGATTCGAC                                                                                <  SRR4302532.677918/94‑1 (MQ=60)
            CATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGAG                                                                                >  SRR4302532.3203500/1‑94 (MQ=60)
            CATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGAC                                                                                >  SRR4302532.1056772/1‑94 (MQ=60)
             ATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACG                                                                               <  SRR4302532.5565429/94‑1 (MQ=60)
                 ATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCT                                                                           >  SRR4302532.1173335/1‑94 (MQ=60)
                  TTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTC                                                                          <  SRR4302532.191335/94‑1 (MQ=60)
                   TTCAGCGGCAAAACAGGCTGGGGTAAATGGTGGGGAAAAAACTGGATCAGAACGGATATTGGCGATTACGGCAACTCTGGATTGCACGATCTCA                                                                         >  SRR4302532.3993344/1‑94 (MQ=60)
                     CAGCGACAAAACAGGCTGGTATAAATGGTGGGAAAAAAACTGGATCAAAACGGATATCGGCGATTTCGCCAATCCTGGATTCGACGATCTCGCT                                                                       <  SRR4302532.3345679/94‑1 (MQ=60)
                     CAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACT                                                                       >  SRR4302532.4451013/1‑94 (MQ=60)
                     CAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACT                                                                       <  SRR4302532.3907390/94‑1 (MQ=60)
                     CAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACT                                                                       <  SRR4302532.5984221/94‑1 (MQ=60)
                       GCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTAT                                                                     <  SRR4302532.4771009/94‑1 (MQ=60)
                       GCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTAT                                                                     <  SRR4302532.6282992/94‑1 (MQ=60)
                        CGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATG                                                                    >  SRR4302532.4926049/1‑94 (MQ=60)
                        CGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATG                                                                    >  SRR4302532.1162327/1‑94 (MQ=60)
                         GACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGT                                                                   <  SRR4302532.2081408/94‑1 (MQ=60)
                         GACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGT                                                                   >  SRR4302532.1001377/1‑94 (MQ=60)
                         GACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGT                                                                   >  SRR4302532.4183680/1‑94 (MQ=60)
                          ACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTC                                                                  <  SRR4302532.4802865/94‑1 (MQ=60)
                          ACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTC                                                                  >  SRR4302532.6059892/1‑94 (MQ=60)
                           CAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCG                                                                 >  SRR4302532.4030437/1‑94 (MQ=60)
                            AAAACAGGCTGGGATAAATGGTGGGGGAAAAACTGGATCAGAACGGATATCGGTGATTACGACAACCCTGGATTCGATGTTATCACTATGTCGG                                                                >  SRR4302532.5467954/1‑94 (MQ=60)
                             AAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCT                                                               >  SRR4302532.5277456/1‑94 (MQ=60)
                              AACAGGCTGGGGTAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTA                                                              >  SRR4302532.2832407/1‑94 (MQ=60)
                              AACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTA                                                              <  SRR4302532.5790189/94‑1 (MQ=60)
                               ACAGGCTGGGATAAATGGTGGGGAAAAAACTTGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAG                                                             <  SRR4302532.1892620/94‑1 (MQ=60)
                                CAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGC                                                            <  SRR4302532.809371/94‑1 (MQ=60)
                                 AGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGAAAACCCTGGATTCGACGATCTCACTATCTTGCTAGCC                                                           >  SRR4302532.1525744/1‑94 (MQ=60)
                                  GGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCT                                                          <  SRR4302532.4877845/94‑1 (MQ=60)
                                   GCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTT                                                         <  SRR4302532.14744/94‑1 (MQ=60)
                                    CTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTT                                                        <  SRR4302532.4380302/94‑1 (MQ=60)
                                    CTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCAATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTT                                                        <  SRR4302532.5963258/94‑1 (MQ=60)
                                     TGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTT                                                       >  SRR4302532.3258476/1‑94 (MQ=60)
                                       GTATAAATGGTGGGTAAAAAACTGTATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTG                                                     <  SRR4302532.5053148/94‑1 (MQ=60)
                                       GGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTG                                                     <  SRR4302532.6003836/94‑1 (MQ=60)
                                       GGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTG                                                     <  SRR4302532.2940949/94‑1 (MQ=60)
                                       GGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTG                                                     <  SRR4302532.4983634/94‑1 (MQ=60)
                                       GGATAAATGGTGGGGAAAAAACTGGATCAGAACGCATATCGGCGATTACGACAACCCTGGGTTCGACGATCTCACTATGTCGCTAGCCTTTTTG                                                     <  SRR4302532.575439/94‑1 (MQ=60)
                                        GATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGC                                                    >  SRR4302532.38566/1‑94 (MQ=60)
                                         ATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCG                                                   >  SRR4302532.804313/1‑94 (MQ=60)
                                          TAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCG                                                  >  SRR4302532.6275895/1‑94 (MQ=60)
                                           AAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTCCCGG                                                 >  SRR4302532.3398620/1‑94 (MQ=60)
                                            AATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGA                                                >  SRR4302532.4482531/1‑94 (MQ=60)
                                            AATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGA                                                >  SRR4302532.3300997/1‑94 (MQ=60)
                                             ATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGAT                                               <  SRR4302532.2882976/94‑1 (MQ=60)
                                             ATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGAT                                               >  SRR4302532.1717543/1‑94 (MQ=60)
                                               GGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATAT                                             <  SRR4302532.3524523/94‑1 (MQ=60)
                                                 TGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCA                                           <  SRR4302532.2625115/94‑1 (MQ=60)
                                                 TGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCA                                           <  SRR4302532.5183227/94‑1 (MQ=60)
                                                 TGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCA                                           >  SRR4302532.3145299/1‑94 (MQ=60)
                                                   GGGAAAAAACTGGATCAGAACGGATATCGTCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAA                                         <  SRR4302532.3121810/94‑1 (MQ=60)
                                                   GGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAA                                         <  SRR4302532.1713607/94‑1 (MQ=60)
                                                   GGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAA                                         <  SRR4302532.4410837/94‑1 (MQ=60)
                                                    GGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAA                                        <  SRR4302532.864949/94‑1 (MQ=60)
                                                    GGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAA                                        <  SRR4302532.4242256/94‑1 (MQ=60)
                                                     GAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAAC                                       >  SRR4302532.2481391/1‑94 (MQ=60)
                                                      AAAAAACTGGATCAGAACGTATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACC                                      <  SRR4302532.637805/94‑1 (MQ=60)
                                                      AAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACC                                      >  SRR4302532.3186025/1‑94 (MQ=60)
                                                      AAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACC                                      >  SRR4302532.569085/1‑94 (MQ=60)
                                                      AAAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACC                                      >  SRR4302532.4846522/1‑94 (MQ=60)
                                                       AAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCG                                     <  SRR4302532.5385382/94‑1 (MQ=60)
                                                       AAAAACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATACAAACCG                                     >  SRR4302532.1100858/1‑94 (MQ=60)
                                                          AACTTGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAT                                  <  SRR4302532.1753310/94‑1 (MQ=60)
                                                          AACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAT                                  >  SRR4302532.5226218/1‑94 (MQ=60)
                                                          AACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAT                                  <  SRR4302532.1338937/94‑1 (MQ=60)
                                                          AACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAAT                                  <  SRR4302532.172421/94‑1 (MQ=60)
                                                           ACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATC                                 <  SRR4302532.3104530/94‑1 (MQ=60)
                                                           ACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATC                                 >  SRR4302532.693416/1‑94 (MQ=60)
                                                           ACTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATC                                 >  SRR4302532.693417/1‑94 (MQ=60)
                                                            CTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA                                >  SRR4302532.2212632/1‑94 (MQ=60)
                                                            CTGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA                                <  SRR4302532.1725629/94‑1 (MQ=60)
                                                             TGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAA                               >  SRR4302532.3444540/1‑94 (MQ=60)
                                                             TGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAA                               >  SRR4302532.4597969/1‑94 (MQ=60)
                                                             TGGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAA                               >  SRR4302532.516543/1‑94 (MQ=60)
                                                              GGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCTGGATATCAAAACCGAATCAAC                              <  SRR4302532.5898007/94‑1 (MQ=60)
                                                              GGATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAAC                              <  SRR4302532.2855327/94‑1 (MQ=60)
                                                               GATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACT                             >  SRR4302532.3608442/1‑94 (MQ=60)
                                                                ATCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTA                            <  SRR4302532.4458559/94‑1 (MQ=60)
                                                                 TCAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTAC                           >  SRR4302532.3421962/1‑94 (MQ=60)
                                                                  CAGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACC                          <  SRR4302532.3737/94‑1 (MQ=60)
                                                                   AGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCG                         >  SRR4302532.1896235/1‑94 (MQ=60)
                                                                   AGAACGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCC                         >  SRR4302532.1593865/1‑94 (MQ=60)
                                                                       CGGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTC                     >  SRR4302532.1748885/1‑94 (MQ=60)
                                                                        GGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCT                    >  SRR4302532.4256321/1‑94 (MQ=60)
                                                                        GGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCT                    >  SRR4302532.1973685/1‑94 (MQ=60)
                                                                        GGATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCT                    >  SRR4302532.397808/1‑94 (MQ=60)
                                                                          ATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGG                  >  SRR4302532.587100/1‑94 (MQ=60)
                                                                          ATATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGG                  <  SRR4302532.1992750/94‑1 (MQ=60)
                                                                            ATCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTC                >  SRR4302532.834005/1‑94 (MQ=60)
                                                                             TCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCT               <  SRR4302532.1343035/94‑1 (MQ=60)
                                                                             TCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCT               <  SRR4302532.3505689/94‑1 (MQ=60)
                                                                             TCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCT               <  SRR4302532.6101460/94‑1 (MQ=60)
                                                                             TCGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTCTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCT               >  SRR4302532.2653876/1‑94 (MQ=60)
                                                                              CGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTG              >  SRR4302532.4485195/1‑94 (MQ=60)
                                                                              CGGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTG              <  SRR4302532.3033487/94‑1 (MQ=60)
                                                                               GGCGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGC             >  SRR4302532.5123858/1‑94 (MQ=60)
                                                                                 CGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCG           <  SRR4302532.3965122/94‑1 (MQ=60)
                                                                                 CGATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCG           >  SRR4302532.5339527/1‑94 (MQ=60)
                                                                                 CGATTACGACAACCCTGGATTCGACGATCTCACTATATCGCTAGCATTTTTGCCGGATATGAAAACCGAATCACCTACTGCTTCTGGGCTGCCG           >  SRR4302532.5339525/1‑94 (MQ=60)
                                                                                  GATTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGG          >  SRR4302532.4666338/1‑94 (MQ=60)
                                                                                    TTACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTG        <  SRR4302532.2117096/94‑1 (MQ=60)
                                                                                      ACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTT      <  SRR4302532.392541/94‑1 (MQ=60)
                                                                                      ACGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTT      <  SRR4302532.392540/94‑1 (MQ=60)
                                                                                       CGACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTC     >  SRR4302532.2305966/1‑94 (MQ=60)
                                                                                        GACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCT    <  SRR4302532.148408/94‑1 (MQ=60)
                                                                                        GACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCT    <  SRR4302532.298205/94‑1 (MQ=60)
                                                                                         ACAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTA   <  SRR4302532.6300635/94‑1 (MQ=60)
                                                                                          CAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTAT  <  SRR4302532.3551831/94‑1 (MQ=60)
                                                                                          CAACCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTAT  <  SRR4302532.2252576/94‑1 (MQ=60)
                                                                                             |                                                                                          
CTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTAT  >  NC_000913/3736205‑3736388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑