Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,891,703	T→C	L69P (CTG→CCG)	yieE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,891,703	0	T	C	83.4%	623.4 / 120.8	259	L69P (CTG→CCG)	yieE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (28/15); new base C (107/109); total (135/124)
Fisher's exact test for biased strand distribution p-value = 6.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

Only 100 of 271 total aligned reads displayed.

GTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACC  >  NC_000913/3891613‑3891794

gTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGc                                                                                           >  2:1656855/1‑93 (MQ=255)

gTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGc                                                                                           <  2:6512272/93‑1 (MQ=255)

 tCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGcc                                                                                          >  2:2689352/1‑93 (MQ=255)

 tCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGcc                                                                                          >  1:3449899/1‑93 (MQ=255)

    aaGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGaa                                                                                       >  1:5164434/1‑93 (MQ=255)

     aGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGaaa                                                                                      <  2:3095624/93‑1 (MQ=255)

     aGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGaaa                                                                                      <  1:3264258/93‑1 (MQ=255)

      gCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAg                                                                                     >  1:4575760/1‑93 (MQ=255)

      gCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAg                                                                                     <  2:1825789/93‑1 (MQ=255)

       ccGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAgg                                                                                    <  2:568696/93‑1 (MQ=255)

        cGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGt                                                                                   >  2:4400495/1‑93 (MQ=255)

         gTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTa                                                                                  <  1:5774172/93‑1 (MQ=255)

         gTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTa                                                                                  >  1:4079170/1‑93 (MQ=255)

         gTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTa                                                                                  <  2:84248/93‑1 (MQ=255)

          ttttCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTaa                                                                                 >  1:448125/1‑93 (MQ=255)

          ttttCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTaa                                                                                 <  2:5331860/93‑1 (MQ=255)

           tttCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTaaa                                                                                <  2:3752809/93‑1 (MQ=255)

           tttCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTaaa                                                                                >  2:5454642/1‑93 (MQ=255)

            ttCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAGAGGTAAAc                                                                               >  2:2391785/1‑93 (MQ=255)

             tCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAAcc                                                                              >  1:5987838/1‑93 (MQ=255)

               tGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCgg                                                                            >  1:3519436/1‑93 (MQ=255)

               tGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCgg                                                                            >  1:2842956/1‑93 (MQ=255)

                ggCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGt                                                                           <  2:5556054/93‑1 (MQ=255)

                ggCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGt                                                                           <  1:2309006/93‑1 (MQ=255)

                 gCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGtt                                                                          <  1:2754441/93‑1 (MQ=255)

                 gCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGtt                                                                          <  2:2173027/93‑1 (MQ=255)

                 gCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGtt                                                                          >  1:6506633/1‑93 (MQ=255)

                  cGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCGCCCCGCCGAAAGGTAAACCGGttt                                                                         <  1:4827779/93‑1 (MQ=255)

                    tCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCCCCCCGCCGAAAGGTAAACCGGTTTTc                                                                       <  2:2870515/93‑1 (MQ=255)

                    tCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTc                                                                       >  1:1363091/1‑93 (MQ=255)

                    tCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTc                                                                       >  1:50019/1‑93 (MQ=255)

                    tCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTc                                                                       >  1:548339/1‑93 (MQ=255)

                     ccAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCa                                                                      <  1:2896957/93‑1 (MQ=255)

                     ccAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCa                                                                      <  2:1978908/93‑1 (MQ=255)

                      cAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAg                                                                     <  1:1816033/93‑1 (MQ=255)

                       agagGTTTACTCGCAGAACTGATGTTCACGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGCAGACCGGTTTTCAGt                                                                    >  2:1054826/1‑93 (MQ=255)

                        gagGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTg                                                                   <  2:3577811/93‑1 (MQ=255)

                        gagGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTg                                                                   <  2:869772/93‑1 (MQ=255)

                         agGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGa                                                                  >  1:4736191/1‑93 (MQ=255)

                          ggTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGAt                                                                 <  2:2631991/93‑1 (MQ=255)

                          ggTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGAt                                                                 <  1:6082106/93‑1 (MQ=255)

                           gTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGACGAAAGGTAAACCGGTTTTCAGTGATa                                                                <  1:104882/93‑1 (MQ=255)

                            tttACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATaa                                                               >  1:2501060/1‑93 (MQ=255)

                             ttACTCGCAGAACTGATGTTCATGCTGTATGGCATTAGCGCATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATaaa                                                              >  1:6428964/1‑93 (MQ=255)

                                cTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAAt                                                           <  1:4957430/93‑1 (MQ=255)

                                 tCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAAtt                                                          <  2:5053285/93‑1 (MQ=255)

                                  cGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAAttt                                                         <  1:1993446/93‑1 (MQ=255)

                                  cGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAAttt                                                         <  2:240211/93‑1 (MQ=255)

                                  cGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAAttt                                                         >  1:3913003/1‑93 (MQ=255)

                                  cGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAAttt                                                         >  1:857992/1‑93 (MQ=255)

                                      gAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCt                                                     >  1:4401215/1‑93 (MQ=255)

                                      gAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCt                                                     <  1:6216219/93‑1 (MQ=255)

                                      gAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCt                                                     >  1:2585461/1‑93 (MQ=255)

                                       aaCTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCtt                                                    >  1:5513811/1‑93 (MQ=255)

                                        aCTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTc                                                   >  1:1647660/1‑93 (MQ=255)

                                        aCTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTc                                                   >  2:5201569/1‑93 (MQ=255)

                                          tGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGt                                                 >  1:5764630/1‑93 (MQ=255)

                                           gATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGtt                                                >  1:2142617/1‑93 (MQ=255)

                                            aTGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGttt                                               >  2:3137706/1‑93 (MQ=255)

                                             tGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGtttt                                              <  1:363849/93‑1 (MQ=255)

                                             tGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGtttt                                              <  2:1254806/93‑1 (MQ=255)

                                             tGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGtttt                                              >  1:5144538/1‑93 (MQ=255)

                                              gTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTc                                             <  1:3084299/93‑1 (MQ=255)

                                              gTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTc                                             <  2:3940448/93‑1 (MQ=255)

                                              gTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTc                                             >  1:295005/1‑93 (MQ=255)

                                               ttCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTcc                                            <  1:3365588/93‑1 (MQ=255)

                                                tCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCa                                           >  2:4843739/1‑93 (MQ=255)

                                                tCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCa                                           <  2:62/93‑1 (MQ=255)

                                                 cATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAt                                          >  1:5702245/1‑93 (MQ=255)

                                                  aTGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAtt                                         <  1:6571059/93‑1 (MQ=255)

                                                  aTGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAtt                                         <  1:6114474/93‑1 (MQ=255)

                                                   tGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAttt                                        <  2:64147/93‑1 (MQ=255)

                                                   tGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAttt                                        <  1:5300984/93‑1 (MQ=255)

                                                      tGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCt                                     >  2:3532972/1‑93 (MQ=255)

                                                       gTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTa                                    <  1:1227408/93‑1 (MQ=255)

                                                        tATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTAt                                   <  2:2557590/93‑1 (MQ=255)

                                                         aTGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTTCATTTCCTATg                                  <  2:6066669/93‑1 (MQ=255)

                                                          tGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGc                                 >  2:1003128/1‑93 (MQ=255)

                                                          tGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGGTTTCAGTGATAAAAAATTGTCTTAGTTTTCCATTTACTATGc                                 >  2:194712/1‑93 (MQ=255)

                                                           ggCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGcc                                >  2:2788072/1‑93 (MQ=255)

                                                             cATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCgg                              <  2:3914492/93‑1 (MQ=255)

                                                             cATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCgg                              <  1:426329/93‑1 (MQ=255)

                                                              aTTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCggg                             >  1:1487620/1‑93 (MQ=255)

                                                               ttGGCTAATTGCCGGAAATCGTCACCCCTCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGa                            >  2:1321255/1‑93 (MQ=255)

                                                               ttGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGa                            <  2:1355489/93‑1 (MQ=255)

                                                               ttGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGa                            >  1:748200/1‑93 (MQ=255)

                                                                tGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGaa                           <  2:5877038/93‑1 (MQ=255)

                                                                  gCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAata                         <  2:1442064/93‑1 (MQ=255)

                                                                      aTTGCCGGAAATCGTCGCCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGt                     <  2:433126/93‑1 (MQ=255)

                                                                      aTTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGt                     >  2:5938986/1‑93 (MQ=255)

                                                                      aTTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGt                     <  2:4035995/93‑1 (MQ=255)

                                                                       ttGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGtt                    <  2:4245271/93‑1 (MQ=255)

                                                                              aaaTCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTtggcgtgg             >  1:5573565/1‑93 (MQ=255)

                                                                               aaTCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTtggcgtggc            >  1:6305418/1‑93 (MQ=255)

                                                                                aTCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTtggcgtggcg           >  1:4237779/1‑93 (MQ=255)

                                                                                 tCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTggcgtggcgt          >  2:3281745/1‑93 (MQ=255)

                                                                                 tCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTggcgtggcgt          <  2:1503100/93‑1 (MQ=255)

                                                                                    tCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTaa       >  2:4721905/1‑93 (MQ=255)

                                                                                     cACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTaac      >  1:4185690/1‑93 (MQ=255)

                                                                                      aCCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTaaca     >  1:1977856/1‑93 (MQ=255)

GTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACC  >  NC_000913/3891613‑3891794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCGTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCG  >  NC_000913/3891610‑3891795

ATCGTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCC                                                                                              <  SRR4302547.3886724/94‑1 (MQ=60)

ATCGTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCC                                                                                              >  SRR4302547.1318213/1‑94 (MQ=60)

ATCGTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCC                                                                                              >  SRR4302547.1048839/1‑94 (MQ=60)

 TCGTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCG                                                                                             <  SRR4302547.897354/94‑1 (MQ=60)

  CGTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGC                                                                                            <  SRR4302547.467224/94‑1 (MQ=60)

   GTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCC                                                                                           >  SRR4302547.3520221/1‑94 (MQ=60)

   GTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCC                                                                                           >  SRR4302547.6270661/1‑94 (MQ=60)

     CAAAGCCGTTTTCTGGCGTCCAGAGGGTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGA                                                                                         >  SRR4302547.2354917/1‑94 (MQ=60)

      AAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCATAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAA                                                                                        <  SRR4302547.2959852/94‑1 (MQ=60)

      AAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAA                                                                                        >  SRR4302547.5270767/1‑94 (MQ=60)

      AAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAA                                                                                        <  SRR4302547.884529/94‑1 (MQ=60)

       AAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGGCGAAA                                                                                       >  SRR4302547.3330893/1‑94 (MQ=60)

       AAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAA                                                                                       >  SRR4302547.1346181/1‑94 (MQ=60)

        AGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAG                                                                                      >  SRR4302547.4670473/1‑94 (MQ=60)

        AGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAG                                                                                      <  SRR4302547.3329554/94‑1 (MQ=60)

          CCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGT                                                                                    >  SRR4302547.4181331/1‑94 (MQ=60)

           CGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTA                                                                                   >  SRR4302547.4162066/1‑94 (MQ=60)

           CGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTA                                                                                   >  SRR4302547.6239365/1‑94 (MQ=60)

           CGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTA                                                                                   <  SRR4302547.4487577/94‑1 (MQ=60)

            GTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAA                                                                                  <  SRR4302547.2065301/94‑1 (MQ=60)

            GTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAA                                                                                  >  SRR4302547.456447/1‑94 (MQ=60)

            GTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAA                                                                                  >  SRR4302547.5234509/1‑94 (MQ=60)

            GTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAA                                                                                  <  SRR4302547.5893602/94‑1 (MQ=60)

              TTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAAC                                                                                <  SRR4302547.4119373/94‑1 (MQ=60)

               TTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACC                                                                               >  SRR4302547.6112071/1‑94 (MQ=60)

                TCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCG                                                                              <  SRR4302547.2907082/94‑1 (MQ=60)

                TCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCG                                                                              <  SRR4302547.5702173/94‑1 (MQ=60)

                 CTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGG                                                                             >  SRR4302547.4344349/1‑94 (MQ=60)

                 CTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGG                                                                             >  SRR4302547.3591278/1‑94 (MQ=60)

                 CTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGG                                                                             >  SRR4302547.667993/1‑94 (MQ=60)

                 CTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGG                                                                             >  SRR4302547.2899765/1‑94 (MQ=60)

                  TGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGT                                                                            >  SRR4302547.5386252/1‑94 (MQ=60)

                   GGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTT                                                                           <  SRR4302547.2355828/94‑1 (MQ=60)

                   GGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTT                                                                           <  SRR4302547.4517647/94‑1 (MQ=60)

                   GGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTT                                                                           >  SRR4302547.6642086/1‑94 (MQ=60)

                    GCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTT                                                                          <  SRR4302547.2809659/94‑1 (MQ=60)

                    GCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTT                                                                          <  SRR4302547.4595630/94‑1 (MQ=60)

                     CGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCGCCCCGCCGAAAGGTAAACCGGTTTT                                                                         <  SRR4302547.4927880/94‑1 (MQ=60)

                     CGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTT                                                                         <  SRR4302547.4879488/94‑1 (MQ=60)

                     CGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTT                                                                         <  SRR4302547.2829135/94‑1 (MQ=60)

                      GTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTC                                                                        >  SRR4302547.1390441/1‑94 (MQ=60)

                      GTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTC                                                                        >  SRR4302547.558241/1‑94 (MQ=60)

                      GTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTC                                                                        >  SRR4302547.50951/1‑94 (MQ=60)

                        CCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGTGAATTGCCGGGAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTGAG                                                                      >  SRR4302547.6247741/1‑94 (MQ=60)

                        CCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAG                                                                      >  SRR4302547.5848142/1‑94 (MQ=60)

                        CCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAG                                                                      <  SRR4302547.2954727/94‑1 (MQ=60)

                         CAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGT                                                                     >  SRR4302547.5082/1‑94 (MQ=60)

                         CAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGT                                                                     <  SRR4302547.1851918/94‑1 (MQ=60)

                          AGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTG                                                                    <  SRR4302547.2988280/94‑1 (MQ=60)

                           GAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGA                                                                   >  SRR4302547.3565729/1‑94 (MQ=60)

                           GAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGA                                                                   >  SRR4302547.4834349/1‑94 (MQ=60)

                           GAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGA                                                                   <  SRR4302547.4807629/94‑1 (MQ=60)

                             GGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGCTTTCAGTGATA                                                                 >  SRR4302547.6691315/1‑94 (MQ=60)

                             GGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATA                                                                 <  SRR4302547.6208294/94‑1 (MQ=60)

                             GGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCAATCCGCCGAAAGGTAAACCGGTTTTCAGTGATA                                                                 >  SRR4302547.586938/1‑94 (MQ=60)

                              GTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAA                                                                >  SRR4302547.6396618/1‑94 (MQ=60)

                              GTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAA                                                                >  SRR4302547.2551589/1‑94 (MQ=60)

                              GTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAA                                                                >  SRR4302547.6133116/1‑94 (MQ=60)

                              GTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGACGAAAGGTAAACCGGTTTTCAGTGATAA                                                                <  SRR4302547.106794/94‑1 (MQ=60)

                               TTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTAGCGCATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAA                                                               >  SRR4302547.6562989/1‑94 (MQ=60)

                                 TACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAA                                                             <  SRR4302547.5977362/94‑1 (MQ=60)

                                  ACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAAT                                                            >  SRR4302547.2895554/1‑94 (MQ=60)

                                   CTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATT                                                           <  SRR4302547.1299194/94‑1 (MQ=60)

                                   CTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATT                                                           <  SRR4302547.5060027/94‑1 (MQ=60)

                                    TCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTT                                                          >  SRR4302547.874389/1‑94 (MQ=60)

                                    TCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTT                                                          >  SRR4302547.750621/1‑94 (MQ=60)

                                    TCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTT                                                          >  SRR4302547.5254487/1‑94 (MQ=60)

                                    TCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTT                                                          >  SRR4302547.3992565/1‑94 (MQ=60)

                                    TCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTT                                                          <  SRR4302547.2155835/94‑1 (MQ=60)

                                     CGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCCCCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTG                                                         <  SRR4302547.3477033/94‑1 (MQ=60)

                                     CGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTG                                                         <  SRR4302547.2033088/94‑1 (MQ=60)

                                        AGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCT                                                      >  SRR4302547.4491846/1‑94 (MQ=60)

                                        AGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCT                                                      >  SRR4302547.2637628/1‑94 (MQ=60)

                                         GAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTT                                                     >  SRR4302547.6439212/1‑94 (MQ=60)

                                         GAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTT                                                     <  SRR4302547.6345315/94‑1 (MQ=60)

                                         GAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTT                                                     >  SRR4302547.5627641/1‑94 (MQ=60)

                                          AACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTC                                                    >  SRR4302547.1680196/1‑94 (MQ=60)

                                          AACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTC                                                    >  SRR4302547.2426298/1‑94 (MQ=60)

                                          AACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTC                                                    >  SRR4302547.3018244/1‑94 (MQ=60)

                                           ACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCG                                                   >  SRR4302547.89695/1‑94 (MQ=60)

                                           ACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCG                                                   <  SRR4302547.4212106/94‑1 (MQ=60)

                                           ACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCG                                                   <  SRR4302547.6365522/94‑1 (MQ=60)

                                            CTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGT                                                  >  SRR4302547.5883851/1‑94 (MQ=60)

                                             TGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTT                                                 >  SRR4302547.5669972/1‑94 (MQ=60)

                                             TGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTT                                                 >  SRR4302547.2185352/1‑94 (MQ=60)

                                              GATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTT                                                <  SRR4302547.6182412/94‑1 (MQ=60)

                                              GATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGCTTTCAGTGATAAAAATTTGCCTTCGTTT                                                >  SRR4302547.1871679/1‑94 (MQ=60)

                                               ATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTT                                               <  SRR4302547.4277294/94‑1 (MQ=60)

                                               ATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTT                                               >  SRR4302547.5250503/1‑94 (MQ=60)

                                                TGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTC                                              <  SRR4302547.370609/94‑1 (MQ=60)

                                                TGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTC                                              >  SRR4302547.300487/1‑94 (MQ=60)

                                                TGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTC                                              >  SRR4302547.3160096/1‑94 (MQ=60)

                                                 GTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCC                                             <  SRR4302547.3145518/94‑1 (MQ=60)

                                                  TTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCA                                            <  SRR4302547.6543208/94‑1 (MQ=60)

                                                  TTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCA                                            <  SRR4302547.3433942/94‑1 (MQ=60)

                                                  TTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGGTTTCAGTGATAAAAATTTGCCTTCGTTTTCCA                                            >  SRR4302547.48860/1‑94 (MQ=60)

                                                   TCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAT                                           >  SRR4302547.5820037/1‑94 (MQ=60)

                                                   TCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAT                                           >  SRR4302547.5646552/1‑94 (MQ=60)

                                                    CATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATT                                          >  SRR4302547.4344961/1‑94 (MQ=60)

                                                    CATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATT                                          >  SRR4302547.2475942/1‑94 (MQ=60)

                                                    CATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATT                                          >  SRR4302547.5537764/1‑94 (MQ=60)

                                                    CATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATT                                          <  SRR4302547.3091947/94‑1 (MQ=60)

                                                     ATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTT                                         <  SRR4302547.6707868/94‑1 (MQ=60)

                                                     ATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTT                                         >  SRR4302547.3949180/1‑94 (MQ=60)

                                                     ATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTT                                         <  SRR4302547.6241311/94‑1 (MQ=60)

                                                     ATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTT                                         <  SRR4302547.2720857/94‑1 (MQ=60)

                                                      TGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTC                                        <  SRR4302547.5410346/94‑1 (MQ=60)

                                                        CTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCT                                      >  SRR4302547.2019139/1‑94 (MQ=60)

                                                        CTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCT                                      <  SRR4302547.505532/94‑1 (MQ=60)

                                                        CTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCT                                      >  SRR4302547.6041960/1‑94 (MQ=60)

                                                        CTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCT                                      <  SRR4302547.4450512/94‑1 (MQ=60)

                                                         TGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTA                                     <  SRR4302547.685244/94‑1 (MQ=60)

                                                          GTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTAT                                    <  SRR4302547.1893036/94‑1 (MQ=60)

                                                          GTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTAT                                    >  SRR4302547.4589426/1‑94 (MQ=60)

                                                          GTATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTAT                                    <  SRR4302547.1252173/94‑1 (MQ=60)

                                                           TATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATG                                   <  SRR4302547.5871259/94‑1 (MQ=60)

                                                           TATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATG                                   <  SRR4302547.4017585/94‑1 (MQ=60)

                                                           TATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATG                                   >  SRR4302547.788331/1‑94 (MQ=60)

                                                            ATGGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGC                                  <  SRR4302547.2192702/94‑1 (MQ=60)

                                                              GGCATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCG                                <  SRR4302547.5076811/94‑1 (MQ=60)

                                                                CATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGG                              >  SRR4302547.5784314/1‑94 (MQ=60)

                                                                CATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGG                              >  SRR4302547.1517316/1‑94 (MQ=60)

                                                                CATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGG                              <  SRR4302547.1414986/94‑1 (MQ=60)

                                                                CATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGG                              >  SRR4302547.3907352/1‑94 (MQ=60)

                                                                CATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGG                              <  SRR4302547.434227/94‑1 (MQ=60)

                                                                 ATTGGCGAATTGCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGA                             >  SRR4302547.762308/1‑94 (MQ=60)

                                                                      CGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATG                        <  SRR4302547.5047184/94‑1 (MQ=60)

                                                                            GCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAATGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCTGGGAATATGGTTGGC                  <  SRR4302547.3605058/94‑1 (MQ=60)

                                                                            GCCGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGC                  <  SRR4302547.3202471/94‑1 (MQ=60)

                                                                              CGGAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGACTATGGTTGGCGT                >  SRR4302547.4749848/1‑94 (MQ=60)

                                                                                GAAATCGTCTCCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGG              >  SRR4302547.6580430/1‑94 (MQ=60)

                                                                                GAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGG              >  SRR4302547.5688490/1‑94 (MQ=60)

                                                                                GAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGG              >  SRR4302547.1621670/1‑94 (MQ=60)

                                                                                GAAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGGTGGCGTGG              >  SRR4302547.123578/1‑94 (MQ=60)

                                                                                 AAATCGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGC             >  SRR4302547.6436507/1‑94 (MQ=60)

                                                                                  AATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCG            >  SRR4302547.4324427/1‑94 (MQ=60)

                                                                                   ATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGT           >  SRR4302547.3448656/1‑94 (MQ=60)

                                                                                   ATCGTCACCCCGCCGAAAGGGAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGT           >  SRR4302547.353527/1‑94 (MQ=60)

                                                                                     CGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTA         <  SRR4302547.2731886/94‑1 (MQ=60)

                                                                                     CGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTA         <  SRR4302547.2620196/94‑1 (MQ=60)

                                                                                     CGTCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTA         <  SRR4302547.1480810/94‑1 (MQ=60)

                                                                                       TCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAAC       >  SRR4302547.1772390/1‑94 (MQ=60)

                                                                                       TCACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAAC       >  SRR4302547.4271028/1‑94 (MQ=60)

                                                                                        CACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACA      >  SRR4302547.2017122/1‑94 (MQ=60)

                                                                                        CACCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACA      <  SRR4302547.5435392/94‑1 (MQ=60)

                                                                                          CCCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAAC    <  SRR4302547.5316455/94‑1 (MQ=60)

                                                                                           CCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACC   >  SRR4302547.5613576/1‑94 (MQ=60)

                                                                                           CCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACC   >  SRR4302547.1702869/1‑94 (MQ=60)

                                                                                           CCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACC   >  SRR4302547.5023292/1‑94 (MQ=60)

                                                                                           CCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACC   <  SRR4302547.4836109/94‑1 (MQ=60)

                                                                                           CCCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACC   <  SRR4302547.1437722/94‑1 (MQ=60)

                                                                                            CCGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCG  >  SRR4302547.6066811/1‑94 (MQ=60)

ATCGTCAAAGCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCG  >  NC_000913/3891610‑3891795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`