Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,896,324 +G coding (698/711 nt) b3718 ← putative isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,896,3241.G99.6% 931.4 / ‑4.9 250V233A (GTG→GCG) b3718putative isomerase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (113/136);  minor base T (1/0);  total (114/136)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 266 total aligned reads displayed.

TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA  >  NC_000913/3896232‑3896410
                                                                                             |                                                                                      
ttCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCa                                                                                         >  2:481864/1‑93 (MQ=255)
ttCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCa                                                                                         <  1:2489907/93‑1 (MQ=255)
ttCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCa                                                                                         >  1:4559455/1‑93 (MQ=255)
 tCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCag                                                                                        <  1:5066284/93‑2 (MQ=255)
  cGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagc                                                                                       <  2:1934228/93‑3 (MQ=255)
  cGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagc                                                                                       <  2:624972/93‑3 (MQ=255)
  cGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagc                                                                                       <  2:5316594/93‑3 (MQ=255)
   gTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGCGTGCTAATTCTGTGGCagag                                                                                      >  1:5253844/1‑90 (MQ=255)
   gTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcg                                                                                      >  2:3173976/1‑90 (MQ=255)
      gTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCt                                                                                   <  1:2454463/93‑1 (MQ=255)
      gTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCt                                                                                   >  2:3746642/1‑93 (MQ=255)
       ttATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCtt                                                                                  <  1:4388228/93‑1 (MQ=255)
            tAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                                                                             >  1:1274962/1‑93 (MQ=255)
             aaaTAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg                                                                            >  1:324469/1‑93 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           >  1:2114675/1‑93 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           <  2:173391/93‑1 (MQ=255)
               ataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCg                                                                          >  1:5478170/1‑93 (MQ=255)
                  atGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAtt                                                                       <  1:2039662/93‑1 (MQ=255)
                  atGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAtt                                                                       <  1:2823258/93‑1 (MQ=255)
                  atGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAtt                                                                       >  2:1093839/1‑93 (MQ=255)
                   tGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTa                                                                      <  1:2615513/93‑1 (MQ=255)
                   tGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTa                                                                      >  1:2710174/1‑93 (MQ=255)
                        aCAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATc                                                                 <  2:3037802/93‑1 (MQ=255)
                        aCAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATc                                                                 >  2:4376820/1‑93 (MQ=255)
                          aGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAg                                                               <  2:144539/93‑1 (MQ=255)
                           ggCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGc                                                              >  2:3137657/1‑93 (MQ=255)
                            gCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCg                                                             >  2:3345648/1‑93 (MQ=255)
                            gCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCg                                                             <  2:4011142/93‑1 (MQ=255)
                              cGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCATCGGCTTTATCAGCGATTACCATCAGCGaa                                                           >  2:6612861/1‑93 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  2:252299/1‑93 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  2:4958243/1‑93 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  1:3855044/1‑93 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  1:3416943/1‑93 (MQ=255)
                                gAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAgg                                                         <  1:2031098/93‑1 (MQ=255)
                                 aGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGa                                                        >  1:4625978/1‑93 (MQ=255)
                                 aGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGa                                                        <  2:754783/93‑1 (MQ=255)
                                 aGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGa                                                        <  1:1457696/93‑1 (MQ=255)
                                  gCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAt                                                       <  2:1154826/93‑1 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     <  1:987516/93‑1 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     <  1:1025611/93‑1 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     >  1:4844597/1‑93 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     >  1:5167344/1‑93 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     <  1:3169860/93‑1 (MQ=255)
                                     tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTTCCATCAGCGAAGGATGCa                                                    <  1:6172011/93‑1 (MQ=255)
                                     tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCa                                                    <  2:5354560/93‑1 (MQ=255)
                                     tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCa                                                    >  1:3646309/1‑93 (MQ=255)
                                      aaTTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAg                                                   <  1:101097/93‑1 (MQ=255)
                                        ttCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCt                                                 <  2:2368219/93‑1 (MQ=255)
                                          cacacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGc                                               >  2:245508/1‑93 (MQ=255)
                                          cacacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGc                                               <  1:3484685/93‑1 (MQ=255)
                                            cacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCaa                                             <  1:1832070/93‑1 (MQ=255)
                                             acaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAac                                            <  2:5864587/93‑1 (MQ=255)
                                             acaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAac                                            <  1:1905292/93‑1 (MQ=255)
                                               aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAacac                                          <  1:5268715/93‑1 (MQ=255)
                                                   ggCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGaa                                      <  2:6457371/93‑1 (MQ=255)
                                                     ccTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGc                                    >  2:505118/1‑93 (MQ=255)
                                                       ttATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTg                                  <  1:3115859/93‑1 (MQ=255)
                                                        tATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTgg                                 <  2:2994071/93‑1 (MQ=255)
                                                         aTTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa                                >  1:293369/1‑93 (MQ=255)
                                                         aTTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa                                <  1:4313543/93‑1 (MQ=255)
                                                              ttAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATc                           >  2:5735374/1‑93 (MQ=255)
                                                              ttAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATc                           >  2:4461603/1‑93 (MQ=255)
                                                               tAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCt                          <  2:382654/93‑1 (MQ=255)
                                                               tAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCt                          >  2:915346/1‑93 (MQ=255)
                                                                aaGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCtt                         <  1:120702/93‑1 (MQ=255)
                                                                aaGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCtt                         <  1:6579259/93‑1 (MQ=255)
                                                                aaGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCtt                         <  1:5699969/93‑1 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        <  1:286065/93‑1 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        <  2:1468815/93‑1 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        <  1:6415123/93‑1 (MQ=255)
                                                                  gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCg                       <  2:5263845/93‑1 (MQ=255)
                                                                   cTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCgg                      <  1:400848/93‑1 (MQ=255)
                                                                   cTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCgg                      >  1:6187416/1‑93 (MQ=255)
                                                                   cTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCgg                      >  2:4056005/1‑93 (MQ=255)
                                                                    ttAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGt                     <  2:2574224/93‑1 (MQ=255)
                                                                     tAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCCAAACGGAAGCTGGAACATCTTCGGTa                    >  1:1745169/1‑93 (MQ=255)
                                                                     tAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTa                    <  1:3839595/93‑1 (MQ=255)
                                                                     tAACCGAGTGC‑AATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTaa                   <  2:2264210/93‑1 (MQ=255)
                                                                       aCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTGCCATCAGCGAAGGATGCAGCTGCAACGCGGAAGCTGGAACATCTTCGGTAAc                  <  1:3302755/93‑1 (MQ=255)
                                                                       aCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAc                  <  1:5724006/93‑1 (MQ=255)
                                                                       aCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAc                  <  1:4967808/93‑1 (MQ=255)
                                                                        ccGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAcc                 <  2:4873143/93‑1 (MQ=255)
                                                                          gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCgg               >  1:612086/1‑93 (MQ=255)
                                                                          gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCgg               >  1:3804844/1‑93 (MQ=255)
                                                                          gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCgg               <  2:4597810/93‑1 (MQ=255)
                                                                          gAGTGCTAATGCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCgg               <  2:3220873/93‑1 (MQ=255)
                                                                           aGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGGAACACGGAAGCTGGCACATCTTCGGTAACCGGa              >  1:4195334/1‑93 (MQ=255)
                                                                           aGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGa              <  2:5623997/93‑1 (MQ=255)
                                                                            gTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAc             <  2:1513834/93‑1 (MQ=255)
                                                                             tGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAcc            <  2:4969078/93‑1 (MQ=255)
                                                                             tGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAcc            >  2:2151375/1‑93 (MQ=255)
                                                                             tGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAcc            <  2:5551712/93‑1 (MQ=255)
                                                                             tGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAcc            >  1:6057507/1‑93 (MQ=255)
                                                                              gCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCt           >  2:420372/1‑93 (MQ=255)
                                                                               cTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCtt          <  1:3626492/93‑1 (MQ=255)
                                                                               cTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCtt          <  2:4434216/93‑1 (MQ=255)
                                                                               cTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCtt          <  1:2324222/93‑1 (MQ=255)
                                                                                tAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTg         <  2:5996091/93‑1 (MQ=255)
                                                                                tAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTg         >  2:2948587/1‑93 (MQ=255)
                                                                                tAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTg         <  1:2285017/93‑1 (MQ=255)
                                                                                             |                                                                                      
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA  >  NC_000913/3896232‑3896410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  >  NC_000913/3896231‑3896417
                                                                                             |                                                                                              
TTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA                                                                                                >  SRR4302547.4653781/1‑94 (MQ=60)
TTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA                                                                                                <  SRR4302547.1773848/94‑1 (MQ=60)
 TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑G                                                                                              <  SRR4302547.2540211/94‑1 (MQ=60)
  TCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgc                                                                                              <  SRR4302547.5170821/94‑3 (MQ=60)
    GTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGG                                                                                            <  SRR4302547.5352201/94‑1 (MQ=60)
     TAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGC                                                                                           >  SRR4302547.4587698/1‑94 (MQ=60)
      AGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCT                                                                                          <  SRR4302547.5372981/94‑1 (MQ=60)
      AGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCT                                                                                          <  SRR4302547.5126238/94‑1 (MQ=60)
       GTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTT                                                                                         <  SRR4302547.2504069/94‑1 (MQ=60)
        TTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTT                                                                                        <  SRR4302547.4478513/94‑1 (MQ=60)
         TATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTA                                                                                       >  SRR4302547.2960525/1‑94 (MQ=60)
          ATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAT                                                                                      <  SRR4302547.1364736/94‑1 (MQ=60)
            TTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCA                                                                                    >  SRR4302547.1300643/1‑94 (MQ=60)
             TAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAG                                                                                   >  SRR4302547.330506/1‑94 (MQ=60)
              AAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGC                                                                                  >  SRR4302547.2156813/1‑94 (MQ=60)
               AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 <  SRR4302547.4408547/94‑1 (MQ=60)
               AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 >  SRR4302547.5591297/1‑94 (MQ=60)
               AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 <  SRR4302547.6300803/94‑1 (MQ=60)
                ATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGA                                                                                >  SRR4302547.207166/1‑94 (MQ=60)
                 TAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAT                                                                               >  SRR4302547.1371589/1‑94 (MQ=60)
                 TAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAT                                                                               <  SRR4302547.2937186/94‑1 (MQ=60)
                 TAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAT                                                                               >  SRR4302547.4408036/1‑94 (MQ=60)
                  AATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATT                                                                              >  SRR4302547.388193/1‑94 (MQ=60)
                   ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTA                                                                             >  SRR4302547.2764581/1‑94 (MQ=60)
                   ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTA                                                                             <  SRR4302547.2080338/94‑1 (MQ=60)
                   ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTA                                                                             <  SRR4302547.2879730/94‑1 (MQ=60)
                    TGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAC                                                                            <  SRR4302547.2668216/94‑1 (MQ=60)
                     GAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACC                                                                           <  SRR4302547.5288126/94‑1 (MQ=60)
                      AGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCA                                                                          <  SRR4302547.5858658/94‑1 (MQ=60)
                         ACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTACTTCTGCGGCAGCGGCTCTATCAGCGCTTACCATCA                                                                       >  SRR4302547.2385926/1‑94 (MQ=60)
                           AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     >  SRR4302547.5688104/1‑94 (MQ=60)
                           AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     >  SRR4302547.159981/1‑94 (MQ=60)
                            GGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCG                                                                    <  SRR4302547.4327471/94‑1 (MQ=60)
                            GGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCG                                                                    <  SRR4302547.3698057/94‑1 (MQ=60)
                               CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 >  SRR4302547.3486519/1‑94 (MQ=60)
                               CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 >  SRR4302547.3933513/1‑94 (MQ=60)
                               CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 >  SRR4302547.3153090/1‑94 (MQ=60)
                                 GAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGA                                                               >  SRR4302547.3552270/1‑94 (MQ=60)
                                 GAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGA                                                               <  SRR4302547.2071546/94‑1 (MQ=60)
                                 GAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGA                                                               >  SRR4302547.4721786/1‑94 (MQ=60)
                                  AGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAT                                                              <  SRR4302547.1486825/94‑1 (MQ=60)
                                  AGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAT                                                              <  SRR4302547.1767436/94‑1 (MQ=60)
                                   GCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATG                                                             <  SRR4302547.2530686/94‑1 (MQ=60)
                                    CGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGGGAAGGATGC                                                            >  SRR4302547.1233897/1‑94 (MQ=60)
                                    CGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGC                                                            >  SRR4302547.5273735/1‑94 (MQ=60)
                                    CGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGC                                                            >  SRR4302547.4944993/1‑94 (MQ=60)
                                    CGTAATTCACACATCCAGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCCATTACCATCAGCAAAGGATTC                                                            >  SRR4302547.891816/1‑94 (MQ=60)
                                     GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302547.5264707/1‑94 (MQ=60)
                                     GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302547.2297157/1‑94 (MQ=60)
                                     GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302547.3720679/1‑94 (MQ=60)
                                     GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           <  SRR4302547.1006736/94‑1 (MQ=60)
                                     GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           <  SRR4302547.3232938/94‑1 (MQ=60)
                                     GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302547.1038308/1‑94 (MQ=60)
                                     GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           <  SRR4302547.1045746/94‑1 (MQ=60)
                                      TAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTTCCATCAGCGAAGGATGCAG                                                          <  SRR4302547.6300134/94‑1 (MQ=60)
                                       AATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC                                                         <  SRR4302547.2771249/94‑1 (MQ=60)
                                       AATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC                                                         <  SRR4302547.102959/94‑1 (MQ=60)
                                        ATTCACACATCCGGCCTTTTTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCT                                                        >  SRR4302547.5367185/1‑94 (MQ=60)
                                         TTCACACATCCGGCCTTATTTCTTAAGCTTAGCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTG                                                       <  SRR4302547.4703376/94‑1 (MQ=60)
                                         TTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTG                                                       <  SRR4302547.4808351/94‑1 (MQ=60)
                                         TTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTG                                                       <  SRR4302547.3494063/94‑1 (MQ=60)
                                          TCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGC                                                      <  SRR4302547.2463697/94‑1 (MQ=60)
                                           CACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCA                                                     >  SRR4302547.3715659/1‑94 (MQ=60)
                                           CACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCA                                                     <  SRR4302547.3555772/94‑1 (MQ=60)
                                             CACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAAC                                                   <  SRR4302547.1868275/94‑1 (MQ=60)
                                              ACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA                                                  <  SRR4302547.283521/94‑1 (MQ=60)
                                              ACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA                                                  <  SRR4302547.1942992/94‑1 (MQ=60)
                                                ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                <  SRR4302547.4102835/94‑1 (MQ=60)
                                                ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                <  SRR4302547.5377263/94‑1 (MQ=60)
                                                 TCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGG                                               >  SRR4302547.6120518/1‑94 (MQ=60)
                                                  CCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGA                                              <  SRR4302547.3874686/94‑1 (MQ=60)
                                                   CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             <  SRR4302547.4533878/94‑1 (MQ=60)
                                                   CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             >  SRR4302547.1917340/1‑94 (MQ=60)
                                                     GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCGACACGGAAGC                                           >  SRR4302547.1008810/1‑94 (MQ=60)
                                                     GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGC                                           <  SRR4302547.3117169/94‑1 (MQ=60)
                                                     GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGC                                           <  SRR4302547.2026370/94‑1 (MQ=60)
                                                     GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGC                                           >  SRR4302547.1133882/1‑94 (MQ=60)
                                                       CTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTG                                         <  SRR4302547.2224689/94‑1 (MQ=60)
                                                       CTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTG                                         <  SRR4302547.2109452/94‑1 (MQ=60)
                                                       CTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTG                                         >  SRR4302547.392384/1‑94 (MQ=60)
                                                       CTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTG                                         <  SRR4302547.3914413/94‑1 (MQ=60)
                                                        TTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGG                                        <  SRR4302547.3177621/94‑1 (MQ=60)
                                                        TTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGG                                        >  SRR4302547.4991565/1‑94 (MQ=60)
                                                         TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       <  SRR4302547.330953/94‑1 (MQ=60)
                                                         TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       >  SRR4302547.6517128/1‑94 (MQ=60)
                                                         TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       >  SRR4302547.1213891/1‑94 (MQ=60)
                                                         TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       >  SRR4302547.298817/1‑94 (MQ=60)
                                                          ATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      <  SRR4302547.2691411/94‑1 (MQ=60)
                                                          ATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      <  SRR4302547.4402303/94‑1 (MQ=60)
                                                          ATTTCGTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      <  SRR4302547.551671/94‑1 (MQ=60)
                                                            TTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACA                                    >  SRR4302547.4165258/1‑94 (MQ=60)
                                                              CTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATC                                  >  SRR4302547.3746308/1‑94 (MQ=60)
                                                              CTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATC                                  >  SRR4302547.2347692/1‑94 (MQ=60)
                                                               TTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCT                                 >  SRR4302547.629980/1‑94 (MQ=60)
                                                               TTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCT                                 <  SRR4302547.6217354/94‑1 (MQ=60)
                                                                TAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTT                                <  SRR4302547.3286846/94‑1 (MQ=60)
                                                                TAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTT                                >  SRR4302547.1885271/1‑94 (MQ=60)
                                                                 AAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               <  SRR4302547.5817709/94‑1 (MQ=60)
                                                                 AAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               >  SRR4302547.580830/1‑94 (MQ=60)
                                                                 AAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               <  SRR4302547.6716263/94‑1 (MQ=60)
                                                                 AAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               <  SRR4302547.122902/94‑1 (MQ=60)
                                                                  AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCTATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302547.4994202/94‑1 (MQ=60)
                                                                  AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302547.5183373/94‑1 (MQ=60)
                                                                  AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302547.6548894/94‑1 (MQ=60)
                                                                  AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302547.641594/94‑1 (MQ=60)
                                                                  AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302547.291384/94‑1 (MQ=60)
                                                                   GCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGG                             >  SRR4302547.6315850/1‑94 (MQ=60)
                                                                    CTTAACCGATTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGGAACACGGCAGCTGGGACAGCGTCGGT                            >  SRR4302547.6588778/1‑94 (MQ=60)
                                                                    CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            >  SRR4302547.3958940/1‑94 (MQ=60)
                                                                    CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            <  SRR4302547.408297/94‑1 (MQ=60)
                                                                     TTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCCAAACGGAAGCTGGAACATCTTCGGTA                           >  SRR4302547.1779569/1‑94 (MQ=60)
                                                                     TTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTA                           >  SRR4302547.6188841/1‑94 (MQ=60)
                                                                      TAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAA                          <  SRR4302547.271839/94‑1 (MQ=60)
                                                                      TAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAA                          <  SRR4302547.3917797/94‑1 (MQ=60)
                                                                       AACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAC                         >  SRR4302547.2450523/1‑94 (MQ=60)
                                                                        ACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTGCCATCAGCGAAGGATGCAGCTGCAACGCGGAAGCTGGAACATCTTCGGTAACC                        <  SRR4302547.3369255/94‑1 (MQ=60)
                                                                        ACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACC                        <  SRR4302547.5842244/94‑1 (MQ=60)
                                                                        ACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACC                        <  SRR4302547.5070610/94‑1 (MQ=60)
                                                                         CCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCG                       >  SRR4302547.5466354/1‑94 (MQ=60)
                                                                          CGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGG                      <  SRR4302547.4573811/94‑1 (MQ=60)
                                                                          CGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGG                      >  SRR4302547.3882401/1‑94 (MQ=60)
                                                                          CGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGG                      >  SRR4302547.623441/1‑94 (MQ=60)
                                                                           GAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGGAACACGGAAGCTGGCACATCTTCGGTAACCGGA                     >  SRR4302547.4280896/1‑94 (MQ=60)
                                                                           GAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGA                     >  SRR4302547.3583063/1‑94 (MQ=60)
                                                                           GAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGA                     >  SRR4302547.5622841/1‑94 (MQ=60)
                                                                            AGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAC                    >  SRR4302547.1947955/1‑94 (MQ=60)
                                                                            AGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAC                    <  SRR4302547.3438306/94‑1 (MQ=60)
                                                                             GTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCCGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACC                   >  SRR4302547.3260826/1‑94 (MQ=60)
                                                                             GTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGCCC                   >  SRR4302547.657617/1‑94 (MQ=60)
                                                                             GTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACC                   >  SRR4302547.6183168/1‑94 (MQ=60)
                                                                               GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTT                 <  SRR4302547.1806110/94‑1 (MQ=60)
                                                                               GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTT                 <  SRR4302547.21112/94‑1 (MQ=60)
                                                                               GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTT                 <  SRR4302547.623821/94‑1 (MQ=60)
                                                                               GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTT                 <  SRR4302547.2589312/94‑1 (MQ=60)
                                                                                CTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTG                <  SRR4302547.3700456/94‑1 (MQ=60)
                                                                                CTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTG                <  SRR4302547.2371329/94‑1 (MQ=60)
                                                                                 TAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGC               <  SRR4302547.2331277/94‑1 (MQ=60)
                                                                                    TTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGA            >  SRR4302547.4032860/1‑94 (MQ=60)
                                                                                    TTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGA            <  SRR4302547.3327900/94‑1 (MQ=60)
                                                                                      CTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAC          >  SRR4302547.4784969/1‑94 (MQ=60)
                                                                                      CTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAC          <  SRR4302547.2901443/94‑1 (MQ=60)
                                                                                      CTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAC          >  SRR4302547.3184331/1‑94 (MQ=60)
                                                                                       TGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA         >  SRR4302547.5085977/1‑94 (MQ=60)
                                                                                       TGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA         <  SRR4302547.2533978/94‑1 (MQ=60)
                                                                                       TGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA         <  SRR4302547.749403/94‑1 (MQ=60)
                                                                                        GCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACAT        >  SRR4302547.6076407/1‑94 (MQ=60)
                                                                                         CGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATT       <  SRR4302547.5857927/94‑1 (MQ=60)
                                                                                          GGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTT      <  SRR4302547.2847992/94‑1 (MQ=60)
                                                                                           GCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTT     <  SRR4302547.104979/94‑1 (MQ=60)
                                                                                             G‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  <  SRR4302547.5064125/94‑1 (MQ=60)
                                                                                             |                                                                                              
TTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  >  NC_000913/3896231‑3896417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑