Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,903,427 G→A R257C (CGT→TGT)  bglG ← BglG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,903,4270GA86.3% 814.0 / 89.7 277R257C (CGT→TGT) bglGBglG
Reads supporting (aligned to +/- strand):  ref base G (17/21);  new base A (134/105);  total (151/126)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 278 total aligned reads displayed.

ATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTC  >  NC_000913/3903336‑3903517
                                                                                           |                                                                                          
aTCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGt                                                                                           <  2:3246641/93‑1 (MQ=255)
 tCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACAtt                                                                                          <  2:5683382/93‑1 (MQ=255)
 tCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACAtt                                                                                          >  2:2445952/1‑93 (MQ=255)
 tCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACAtt                                                                                          >  1:5970634/1‑93 (MQ=255)
  ccTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTg                                                                                         >  2:655780/1‑93 (MQ=255)
  ccTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTg                                                                                         >  2:5332438/1‑93 (MQ=255)
   cTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGa                                                                                        <  1:4246642/93‑1 (MQ=255)
    tttACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGAt                                                                                       >  2:4650235/1‑93 (MQ=255)
     ttACTCAGTAATAATATTTCAGTGTTATTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATa                                                                                      >  2:3504642/1‑93 (MQ=255)
      tACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATAc                                                                                     <  1:1083595/93‑1 (MQ=255)
        cTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTg                                                                                   <  2:1165671/93‑1 (MQ=255)
        cTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTg                                                                                   >  2:4270253/1‑93 (MQ=255)
         tCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGc                                                                                  <  1:2980917/93‑1 (MQ=255)
         tCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGc                                                                                  <  2:2865430/93‑1 (MQ=255)
         tCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGc                                                                                  >  1:1502702/1‑93 (MQ=255)
            gTAATAATATTTCAGTGTTCTTTGCGCGCGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCaaa                                                                               >  2:280418/1‑93 (MQ=255)
            gTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCaaa                                                                               <  1:4839098/93‑1 (MQ=255)
            gTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCaaa                                                                               >  1:4231506/1‑93 (MQ=255)
             taataaTATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAAc                                                                              >  1:1037729/1‑93 (MQ=255)
              aataatATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAAcc                                                                             <  1:5267389/93‑1 (MQ=255)
              aataatATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAAcc                                                                             >  2:3275615/1‑93 (MQ=255)
                  atatTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATa                                                                         >  1:5474488/1‑93 (MQ=255)
                   tatTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaa                                                                        >  1:237860/1‑93 (MQ=255)
                    atTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaaa                                                                       >  2:3346584/1‑93 (MQ=255)
                    atTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaaa                                                                       >  1:3599374/1‑93 (MQ=255)
                    atTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaaa                                                                       >  1:3657961/1‑93 (MQ=255)
                    atTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaaa                                                                       >  1:4730601/1‑93 (MQ=255)
                     tttCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaaaa                                                                      <  1:1524049/93‑1 (MQ=255)
                     tttCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaaaa                                                                      <  2:2950211/93‑1 (MQ=255)
                     tttCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATaaaa                                                                      >  1:4087903/1‑93 (MQ=255)
                       tCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAAt                                                                    <  2:2521523/93‑1 (MQ=255)
                        cAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATg                                                                   <  2:2383146/93‑1 (MQ=255)
                          gtgtTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGc                                                                 >  1:1579730/1‑93 (MQ=255)
                          gtgtTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGc                                                                 <  1:3912025/93‑1 (MQ=255)
                           tgtTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCg                                                                >  2:6495820/1‑93 (MQ=255)
                           tgtTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCg                                                                <  1:2582695/93‑1 (MQ=255)
                             ttCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGAt                                                              <  1:4615866/93‑1 (MQ=255)
                              tCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATc                                                             >  2:1035439/1‑93 (MQ=255)
                              tCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATc                                                             <  1:324901/93‑1 (MQ=255)
                              tCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATc                                                             <  1:1879709/93‑1 (MQ=255)
                               cTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATcc                                                            >  1:6418930/1‑93 (MQ=255)
                                tttGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCg                                                           <  1:922336/93‑1 (MQ=255)
                                tttGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCg                                                           <  2:3077104/93‑1 (MQ=255)
                                tttGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCg                                                           <  1:4376981/93‑1 (MQ=255)
                                 ttGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGt                                                          <  2:2667085/93‑1 (MQ=255)
                                 ttGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGt                                                          <  1:3081854/93‑1 (MQ=255)
                                 ttGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGt                                                          <  2:4997748/93‑1 (MQ=255)
                                   gcgcACGCTCTTTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTc                                                        <  2:3700241/93‑1 (MQ=255)
                                      cACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGc                                                     >  2:5386977/1‑93 (MQ=255)
                                        cgcgCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGcac                                                   >  2:4657193/1‑93 (MQ=255)
                                        cgcgCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGcac                                                   <  1:115986/93‑1 (MQ=255)
                                          cgcTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACAt                                                 >  1:5537101/1‑93 (MQ=255)
                                            ctctATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTg                                               >  1:3819944/1‑93 (MQ=255)
                                            ctctATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTg                                               >  1:2263080/1‑93 (MQ=255)
                                            ctctATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTg                                               >  2:6541029/1‑93 (MQ=255)
                                            ctctATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTg                                               >  1:4954674/1‑93 (MQ=255)
                                             tctATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGc                                              >  2:5765432/1‑93 (MQ=255)
                                              ctATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGcc                                             >  2:6311122/1‑93 (MQ=255)
                                               tataTTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCa                                            <  1:6533317/93‑1 (MQ=255)
                                                atatTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCAt                                           <  2:3902340/93‑1 (MQ=255)
                                                  atTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGc                                         <  2:1554666/93‑1 (MQ=255)
                                                  atTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGc                                         <  1:613057/93‑1 (MQ=255)
                                                   tttATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCACGCt                                        >  2:1902056/1‑93 (MQ=255)
                                                      aTGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGc                                     >  2:3725468/1‑93 (MQ=255)
                                                       tGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCg                                    >  1:4911983/1‑93 (MQ=255)
                                                           tAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTa                                >  1:5870119/1‑93 (MQ=255)
                                                           tAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTa                                >  1:2510149/1‑93 (MQ=255)
                                                             aaaaCATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAAt                              <  2:1612758/93‑1 (MQ=255)
                                                             aaaaCATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAAt                              >  1:5390030/1‑93 (MQ=255)
                                                               aaCATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAAttt                            >  1:2018089/1‑93 (MQ=255)
                                                               aaCATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAAttt                            >  2:5918138/1‑93 (MQ=255)
                                                                aCATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAAtttt                           >  2:6607113/1‑93 (MQ=255)
                                                                   tAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGtt                        >  2:85617/1‑93 (MQ=255)
                                                                    aaTCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGttt                       <  2:5841087/93‑1 (MQ=255)
                                                                    aaTCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGttt                       <  2:1218861/93‑1 (MQ=255)
                                                                     aTCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGtttt                      >  1:4996511/1‑93 (MQ=255)
                                                                      tctcTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTa                     <  1:1438145/93‑1 (MQ=255)
                                                                      tctcTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTa                     >  2:784729/1‑93 (MQ=255)
                                                                        tctGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACt                   >  2:400006/1‑93 (MQ=255)
                                                                        tctGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACt                   >  1:5910527/1‑93 (MQ=255)
                                                                          tGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGc                 <  2:1507020/93‑1 (MQ=255)
                                                                          tGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTc                                                        >  2:1601812/1‑54 (MQ=255)
                                                                          tGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGc                 <  2:3828642/93‑1 (MQ=255)
                                                                             gggTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCttg              >  2:443856/1‑93 (MQ=255)
                                                                             gggTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCttg              >  1:2788444/1‑93 (MQ=255)
                                                                              ggTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCttgt             <  2:6137869/93‑1 (MQ=255)
                                                                               gTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCttgtt            <  2:4166128/93‑1 (MQ=255)
                                                                               gTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCttgtt            <  1:1374598/93‑1 (MQ=255)
                                                                               gTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCttgtt            <  1:2198091/93‑1 (MQ=255)
                                                                                tGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCttgttg           <  2:2786956/93‑1 (MQ=255)
                                                                                 gAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTtgttgt          <  1:2363817/93‑1 (MQ=255)
                                                                                 gAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTtgttgt          >  2:2762358/1‑93 (MQ=255)
                                                                                  aaaTTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTa         <  2:480570/93‑1 (MQ=255)
                                                                                    aTTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAAt       >  2:5514847/1‑93 (MQ=255)
                                                                                    aTTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGGAATTTTGTTTTACTGCTTGTTGTAAt       >  2:2110157/1‑93 (MQ=255)
                                                                                     ttttACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTGATg      <  1:2913930/93‑1 (MQ=255)
                                                                                     ttttACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATg      >  1:1787749/1‑93 (MQ=255)
                                                                                     ttttACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATg      <  2:4517862/93‑1 (MQ=255)
                                                                                      tttACATTGATACTGCAAACCAATAAAAATGGCGATACGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGa     <  1:973915/93‑1 (MQ=255)
                                                                                        tACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGAtt   <  1:742999/93‑1 (MQ=255)
                                                                                           |                                                                                          
ATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTC  >  NC_000913/3903336‑3903517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATC  >  NC_000913/3903335‑3903520
                                                                                            |                                                                                             
AATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACAT                                                                                              >  SRR4302547.1270171/1‑94 (MQ=60)
AATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACAT                                                                                              >  SRR4302547.5099706/1‑94 (MQ=60)
 ATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATT                                                                                             >  SRR4302547.6094465/1‑94 (MQ=60)
  TCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTG                                                                                            >  SRR4302547.1126690/1‑94 (MQ=60)
  TCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTG                                                                                            <  SRR4302547.1431487/94‑1 (MQ=60)
    CTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGAT                                                                                          <  SRR4302547.4333508/94‑1 (MQ=60)
    CTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGAT                                                                                          <  SRR4302547.6003916/94‑1 (MQ=60)
      TTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATAC                                                                                        >  SRR4302547.6731912/1‑94 (MQ=60)
       TACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACT                                                                                       <  SRR4302547.1105242/94‑1 (MQ=60)
       TACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACT                                                                                       <  SRR4302547.1548727/94‑1 (MQ=60)
        ACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTG                                                                                      >  SRR4302547.5046993/1‑94 (MQ=60)
         CTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGC                                                                                     >  SRR4302547.2485770/1‑94 (MQ=60)
         CTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGC                                                                                     >  SRR4302547.3812754/1‑94 (MQ=60)
         CTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGC                                                                                     >  SRR4302547.1532660/1‑94 (MQ=60)
          TCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCA                                                                                    <  SRR4302547.3040232/94‑1 (MQ=60)
            AGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAA                                                                                  >  SRR4302547.4318016/1‑94 (MQ=60)
             GTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAAC                                                                                 >  SRR4302547.1058173/1‑94 (MQ=60)
             GTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAAC                                                                                 <  SRR4302547.5019010/94‑1 (MQ=60)
             GTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAAC                                                                                 <  SRR4302547.4257271/94‑1 (MQ=60)
             GTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAAC                                                                                 <  SRR4302547.4939399/94‑1 (MQ=60)
              TAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACC                                                                                <  SRR4302547.268708/94‑1 (MQ=60)
               AATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCA                                                                               >  SRR4302547.5567258/1‑94 (MQ=60)
               AATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCA                                                                               <  SRR4302547.5375897/94‑1 (MQ=60)
                ATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAA                                                                              >  SRR4302547.2613442/1‑94 (MQ=60)
                  AATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATA                                                                            >  SRR4302547.5587570/1‑94 (MQ=60)
                  AATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATA                                                                            <  SRR4302547.929828/94‑1 (MQ=60)
                  AATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATA                                                                            <  SRR4302547.1285708/94‑1 (MQ=60)
                   ATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAA                                                                           >  SRR4302547.242319/1‑94 (MQ=60)
                   ATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAA                                                                           >  SRR4302547.6245637/1‑94 (MQ=60)
                   ATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAA                                                                           >  SRR4302547.3070953/1‑94 (MQ=60)
                    TATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAA                                                                          >  SRR4302547.4828637/1‑94 (MQ=60)
                    TATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAA                                                                          >  SRR4302547.3672765/1‑94 (MQ=60)
                    TATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAA                                                                          >  SRR4302547.3732593/1‑94 (MQ=60)
                     ATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTATATTGATACTGCAAACCAATAAAA                                                                         >  SRR4302547.4427007/1‑94 (MQ=60)
                     ATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACGAATAAAA                                                                         <  SRR4302547.2903375/94‑1 (MQ=60)
                     ATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAA                                                                         >  SRR4302547.4171009/1‑94 (MQ=60)
                      TTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAA                                                                        <  SRR4302547.1554389/94‑1 (MQ=60)
                       TTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAAT                                                                       <  SRR4302547.2068782/94‑1 (MQ=60)
                       TTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAAT                                                                       >  SRR4302547.6088670/1‑94 (MQ=60)
                        TCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATG                                                                      >  SRR4302547.662585/1‑94 (MQ=60)
                        TCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATG                                                                      <  SRR4302547.5203943/94‑1 (MQ=60)
                        TCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATG                                                                      <  SRR4302547.5094518/94‑1 (MQ=60)
                         CAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGG                                                                     >  SRR4302547.323769/1‑94 (MQ=60)
                         CAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGG                                                                     >  SRR4302547.6544112/1‑94 (MQ=60)
                         CAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGG                                                                     >  SRR4302547.194628/1‑94 (MQ=60)
                          AGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGC                                                                    >  SRR4302547.1611169/1‑94 (MQ=60)
                           GTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCG                                                                   <  SRR4302547.4323302/94‑1 (MQ=60)
                           GTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCG                                                                   <  SRR4302547.578472/94‑1 (MQ=60)
                           GTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCG                                                                   <  SRR4302547.3991576/94‑1 (MQ=60)
                            TGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGA                                                                  <  SRR4302547.2634818/94‑1 (MQ=60)
                             GTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGAT                                                                 <  SRR4302547.2265073/94‑1 (MQ=60)
                             GTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGAT                                                                 >  SRR4302547.4986783/1‑94 (MQ=60)
                              TTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATC                                                                <  SRR4302547.4711440/94‑1 (MQ=60)
                               TCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCC                                                               <  SRR4302547.1916852/94‑1 (MQ=60)
                               TCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCC                                                               >  SRR4302547.6552756/1‑94 (MQ=60)
                               TCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCC                                                               <  SRR4302547.330949/94‑1 (MQ=60)
                                CTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCG                                                              <  SRR4302547.5062202/94‑1 (MQ=60)
                                 TTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGT                                                             >  SRR4302547.391405/1‑94 (MQ=60)
                                 TTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGT                                                             <  SRR4302547.4466937/94‑1 (MQ=60)
                                 TTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGT                                                             <  SRR4302547.940154/94‑1 (MQ=60)
                                  TTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTT                                                            <  SRR4302547.3143039/94‑1 (MQ=60)
                                  TTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTT                                                            <  SRR4302547.14584/94‑1 (MQ=60)
                                    cctctttccctacacgacgctcttccgatcttgacatTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCC                                                          <  SRR4302547.1633583/57‑1 (MQ=60)
                                    actctttccctacacgacgctcttccgatcttgacatTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCC                                                          <  SRR4302547.2629029/57‑1 (MQ=60)
                                    TCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCGGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCC                                                          <  SRR4302547.1595393/94‑1 (MQ=60)
                                       CACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCA                                                       <  SRR4302547.6068845/94‑1 (MQ=60)
                                       CACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCA                                                       >  SRR4302547.4057657/1‑94 (MQ=60)
                                         CGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACA                                                     <  SRR4302547.118095/94‑1 (MQ=60)
                                          GCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACAT                                                    >  SRR4302547.5651386/1‑94 (MQ=60)
                                           CGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATT                                                   >  SRR4302547.1014412/1‑94 (MQ=60)
                                            GCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTG                                                  >  SRR4302547.3897780/1‑94 (MQ=60)
                                            GCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTG                                                  >  SRR4302547.5057232/1‑94 (MQ=60)
                                            GCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTG                                                  >  SRR4302547.2308813/1‑94 (MQ=60)
                                             CTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGC                                                 >  SRR4302547.184567/1‑94 (MQ=60)
                                             CTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGC                                                 >  SRR4302547.5608027/1‑94 (MQ=60)
                                             CTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGC                                                 >  SRR4302547.370692/1‑94 (MQ=60)
                                              TCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCC                                                >  SRR4302547.6165486/1‑94 (MQ=60)
                                              TCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCC                                                <  SRR4302547.1528117/94‑1 (MQ=60)
                                               CTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCA                                               >  SRR4302547.4999945/1‑94 (MQ=60)
                                               CTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCA                                               >  SRR4302547.1902304/1‑94 (MQ=60)
                                               CTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCA                                               >  SRR4302547.3022408/1‑94 (MQ=60)
                                               CTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCA                                               <  SRR4302547.4732528/94‑1 (MQ=60)
                                                TATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCAT                                              <  SRR4302547.6669292/94‑1 (MQ=60)
                                                 ATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATG                                             >  SRR4302547.4495130/1‑94 (MQ=60)
                                                   ATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCT                                           >  SRR4302547.3078790/1‑94 (MQ=60)
                                                   ATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCT                                           <  SRR4302547.945042/94‑1 (MQ=60)
                                                   ATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCT                                           <  SRR4302547.624422/94‑1 (MQ=60)
                                                   ATTTATCGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCT                                           >  SRR4302547.957902/1‑94 (MQ=60)
                                                    TTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTT                                          <  SRR4302547.2202967/94‑1 (MQ=60)
                                                     TTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTG                                         <  SRR4302547.1136590/94‑1 (MQ=60)
                                                      TATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGC                                        >  SRR4302547.3735287/1‑94 (MQ=60)
                                                       ATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCG                                       >  SRR4302547.5013698/1‑94 (MQ=60)
                                                        TGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGG                                      >  SRR4302547.4593488/1‑94 (MQ=60)
                                                        TGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGG                                      >  SRR4302547.903805/1‑94 (MQ=60)
                                                        TGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGG                                      >  SRR4302547.5230647/1‑94 (MQ=60)
                                                        TGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGG                                      >  SRR4302547.1703605/1‑94 (MQ=60)
                                                         GGCTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGG                                     >  SRR4302547.2795499/1‑94 (MQ=60)
                                                           CTAAAAACATAATCTCTGCGGGTTAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTA                                   <  SRR4302547.3646471/94‑1 (MQ=60)
                                                           CTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTA                                   >  SRR4302547.5991623/1‑94 (MQ=60)
                                                           CTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTA                                   >  SRR4302547.3581282/1‑94 (MQ=60)
                                                           CTAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTA                                   >  SRR4302547.2560891/1‑94 (MQ=60)
                                                            TAAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAA                                  <  SRR4302547.1187623/94‑1 (MQ=60)
                                                             AAAAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAAT                                 >  SRR4302547.5501346/1‑94 (MQ=60)
                                                               AAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTT                               >  SRR4302547.2058239/1‑94 (MQ=60)
                                                               AAACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTT                               >  SRR4302547.2412516/1‑94 (MQ=60)
                                                                AACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTT                              >  SRR4302547.4080257/1‑94 (MQ=60)
                                                                AACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTT                              <  SRR4302547.481975/94‑1 (MQ=60)
                                                                 ACATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTG                             >  SRR4302547.2252328/1‑94 (MQ=60)
                                                                  CATAATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGT                            >  SRR4302547.2936750/1‑94 (MQ=60)
                                                                     AATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTT                         >  SRR4302547.5099831/1‑94 (MQ=60)
                                                                     AATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTT                         >  SRR4302547.1545674/1‑94 (MQ=60)
                                                                      ATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTA                        >  SRR4302547.1072686/1‑94 (MQ=60)
                                                                      ATCTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTA                        >  SRR4302547.3250134/1‑94 (MQ=60)
                                                                       TCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTAC                       <  SRR4302547.1466905/94‑1 (MQ=60)
                                                                        CTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACT                      >  SRR4302547.2319191/1‑94 (MQ=60)
                                                                        CTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACT                      <  SRR4302547.48016/94‑1 (MQ=60)
                                                                        CTCTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACT                      >  SRR4302547.6032930/1‑94 (MQ=60)
                                                                         TCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTG                     <  SRR4302547.5476911/94‑1 (MQ=60)
                                                                          CTGCGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGC                    >  SRR4302547.5656687/1‑94 (MQ=60)
                                                                            GCTGGTGAAATTTTACATTGATACTGCATACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTT                  <  SRR4302547.6660966/94‑1 (MQ=60)
                                                                            GCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTT                  <  SRR4302547.4395814/94‑1 (MQ=60)
                                                                             CGGGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTG                 >  SRR4302547.2844288/1‑94 (MQ=60)
                                                                               GGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTT               <  SRR4302547.2310319/94‑1 (MQ=60)
                                                                               GGTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTT               <  SRR4302547.5553088/94‑1 (MQ=60)
                                                                                GTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAAatgtcaagatcggaagagcg              <  SRR4302547.6375431/94‑21 (MQ=60)
                                                                                GTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTG              <  SRR4302547.2242248/94‑1 (MQ=60)
                                                                                GTGAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTG              <  SRR4302547.1402163/94‑1 (MQ=60)
                                                                                  GAAATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTA            <  SRR4302547.2411739/94‑1 (MQ=60)
                                                                                    AATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAAT          <  SRR4302547.4213104/94‑1 (MQ=60)
                                                                                    AATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAAT          >  SRR4302547.2489437/1‑94 (MQ=60)
                                                                                    AATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAAT          <  SRR4302547.5590479/94‑1 (MQ=60)
                                                                                     ATTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATG         >  SRR4302547.1823144/1‑94 (MQ=60)
                                                                                      TTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTGATGA        <  SRR4302547.2972052/94‑1 (MQ=60)
                                                                                      TTTTACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGA        >  SRR4302547.1577002/1‑94 (MQ=60)
                                                                                       TTTACATTGATACTGCAAACCAATAAAAATGGCGATACGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGAT       <  SRR4302547.992847/94‑1 (MQ=60)
                                                                                        TTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATT      <  SRR4302547.6309978/94‑1 (MQ=60)
                                                                                         TACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTC     <  SRR4302547.756979/94‑1 (MQ=60)
                                                                                         TACATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTC     >  SRR4302547.2795947/1‑94 (MQ=60)
                                                                                          ACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCA    <  SRR4302547.3920062/94‑1 (MQ=60)
                                                                                           CATTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCAT   >  SRR4302547.4434778/1‑94 (MQ=60)
                                                                                            ATTGATACAGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATC  <  SRR4302547.6463816/94‑1 (MQ=60)
                                                                                            |                                                                                             
AATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATAATCTCTGCGGGTGAAATTTTACGTTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGCACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATC  >  NC_000913/3903335‑3903520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑