Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,928,669 A→G L18L (TTG→CTG)  yieN ← putative 2‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,928,6690AG100.0% 900.8 / NA 242L18L (TTG→CTG) yieNputative 2‑component regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (120/122);  total (120/122)

Only 100 of 244 total aligned reads displayed.

GCGGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAA  >  NC_000913/3928579‑3928758
                                                                                          |                                                                                         
   ggCCAAGGAGGAACACACTTTTACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCTGCTTTTCCAGCGAAc                                                                                      >  1:2783438/1‑93 (MQ=255)
      cAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGc                                                                                   <  1:742740/93‑1 (MQ=255)
       aaGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCt                                                                                  >  2:6198510/1‑93 (MQ=255)
         ggaggaACACGCTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCa                                                                                <  1:1749227/93‑1 (MQ=255)
         ggaggaACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCa                                                                                <  1:1052051/93‑1 (MQ=255)
         ggaggaACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCa                                                                                >  2:4121913/1‑93 (MQ=255)
          gaggaACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAg                                                                               <  1:4915225/93‑1 (MQ=255)
            ggaACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGc                                                                             <  2:1688473/93‑1 (MQ=255)
            ggaACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGc                                                                             >  2:6245538/1‑93 (MQ=255)
               acacacTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCggg                                                                          >  1:2058963/1‑93 (MQ=255)
                 acacTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGaa                                                                        <  2:1217151/93‑1 (MQ=255)
                 acacTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGaa                                                                        >  2:2754474/1‑93 (MQ=255)
                  cacTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGaaa                                                                       <  1:2581724/93‑1 (MQ=255)
                  cacTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGaaa                                                                       <  2:6513971/93‑1 (MQ=255)
                  cacTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGaaa                                                                       <  1:6581081/93‑1 (MQ=255)
                   acTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAAt                                                                      >  1:5230416/1‑93 (MQ=255)
                   acTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAAt                                                                      >  2:3819260/1‑93 (MQ=255)
                    cTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAAtt                                                                     >  2:3656156/1‑93 (MQ=255)
                    cTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAAtt                                                                     >  1:2290997/1‑93 (MQ=255)
                       tctccacTTAATGCAGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCtt                                                                  <  1:4607171/90‑1 (MQ=255)
                       tCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCtt                                                                  <  1:4824838/93‑1 (MQ=255)
                       tCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCtt                                                                  >  1:4018367/1‑93 (MQ=255)
                        caccacTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCttt                                                                 <  1:5949437/93‑1 (MQ=255)
                         cccacTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTc                                                                <  1:3944722/92‑1 (MQ=255)
                         accacTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTc                                                                <  1:3322973/93‑1 (MQ=255)
                         accacTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGACCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTc                                                                >  1:2517751/1‑93 (MQ=255)
                          ccacTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTcc                                                               <  2:454702/93‑1 (MQ=255)
                           cacTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCg                                                              <  1:555451/93‑1 (MQ=255)
                            acTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGc                                                             >  2:1894144/1‑93 (MQ=255)
                              ttAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCta                                                           <  2:5316841/93‑1 (MQ=255)
                               tAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCtaa                                                          >  1:3589921/1‑93 (MQ=255)
                               tAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCtaa                                                          <  1:1127764/93‑1 (MQ=255)
                                 aTGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCtaata                                                        >  2:2121117/1‑93 (MQ=255)
                                  tGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCtaataa                                                       <  1:1022844/93‑1 (MQ=255)
                                  tGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCtaataa                                                       >  2:6447711/1‑93 (MQ=255)
                                   gCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATaaa                                                      <  2:6579386/93‑1 (MQ=255)
                                   gCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATaaa                                                      <  2:4275484/93‑1 (MQ=255)
                                    ccGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAAt                                                     <  2:1405717/93‑1 (MQ=255)
                                     cGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGCAATTCTTTCCGCTAATaaaat                                                    >  1:5163245/1‑91 (MQ=255)
                                      gCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGa                                                   <  2:131393/93‑1 (MQ=255)
                                      gCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGa                                                   >  2:83301/1‑93 (MQ=255)
                                      gCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGa                                                   <  2:5114493/93‑1 (MQ=255)
                                      gCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGa                                                   >  2:5864691/1‑93 (MQ=255)
                                        taataaACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAgg                                                 >  2:4452088/1‑93 (MQ=255)
                                        taataaACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAgg                                                 >  1:4209128/1‑93 (MQ=255)
                                        taataaACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAgg                                                 >  2:3195321/1‑93 (MQ=255)
                                          ataaACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAgggg                                               >  1:6228605/1‑92 (MQ=255)
                                          ataaACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGt                                               >  1:6614058/1‑93 (MQ=255)
                                           taaACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTg                                              <  2:2854334/93‑1 (MQ=255)
                                            aaaCACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGa                                             >  1:4986124/1‑93 (MQ=255)
                                            aaaCACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGa                                             >  1:1973925/1‑93 (MQ=255)
                                             aaCACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAg                                            >  1:6376723/1‑93 (MQ=255)
                                              acacAAGCGGATGGCGTGGCTACGTTCATAAAGCCTCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGc                                           <  1:3222650/93‑1 (MQ=255)
                                              acacAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTTCGCTAATAATTGAGGGTGAGc                                           >  1:2722596/1‑93 (MQ=255)
                                              acacAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGc                                           >  2:3085651/1‑93 (MQ=255)
                                               cacaAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGcc                                          >  2:2354508/1‑93 (MQ=255)
                                                acaAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCa                                         >  1:3375628/1‑93 (MQ=255)
                                                 caAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCAt                                        >  1:5615709/1‑93 (MQ=255)
                                                 caAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCAt                                        >  1:4810193/1‑93 (MQ=255)
                                                  aaGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATa                                       >  1:3672559/1‑93 (MQ=255)
                                                  aaGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATa                                       <  1:1279410/93‑1 (MQ=255)
                                                   aGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATaa                                      <  2:2923686/93‑1 (MQ=255)
                                                   aGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATaa                                      <  2:937975/93‑1 (MQ=255)
                                                     cGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATg                                    <  1:273724/93‑1 (MQ=255)
                                                        aTGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAg                                 >  1:134286/1‑93 (MQ=255)
                                                         tGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGt                                >  1:4477198/1‑93 (MQ=255)
                                                         tGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGt                                >  2:5070823/1‑93 (MQ=255)
                                                         tGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGGGAGCCATAATGAAGt                                >  1:3483095/1‑93 (MQ=255)
                                                           gCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTgg                              >  1:1563610/1‑93 (MQ=255)
                                                           gCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTgg                              >  1:1849551/1‑93 (MQ=255)
                                                           gCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTgg                              <  2:1494968/93‑1 (MQ=255)
                                                             gTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCg                            >  1:878690/1‑93 (MQ=255)
                                                              tGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGt                           <  2:1055433/93‑1 (MQ=255)
                                                               ggCTACGTTCATACAGCCACTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTc                          <  2:5471112/93‑1 (MQ=255)
                                                               ggCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTc                          >  1:4959315/1‑93 (MQ=255)
                                                                gCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTcc                         <  1:2322802/93‑1 (MQ=255)
                                                                gCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTcc                         >  2:2043591/1‑93 (MQ=255)
                                                                  tACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCtt                       <  2:4896405/93‑1 (MQ=255)
                                                                  tACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCtt                       >  1:3539820/1‑93 (MQ=255)
                                                                  tACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCtt                       <  1:5038674/93‑1 (MQ=255)
                                                                  tACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCtt                       <  2:1887756/93‑1 (MQ=255)
                                                                  tACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAAGGAGGGTGAGCCATAATGAAGTGGCGTCCtt                       <  2:2459076/93‑1 (MQ=255)
                                                                    cGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTc                     <  1:5394493/93‑1 (MQ=255)
                                                                    cGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTc                     <  1:5876289/93‑1 (MQ=255)
                                                                    cGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTc                     <  1:6075073/93‑1 (MQ=255)
                                                                    cGTGCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTc                     <  1:2864282/93‑1 (MQ=255)
                                                                     gTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCg                    >  2:4654101/1‑93 (MQ=255)
                                                                     gTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCg                    >  2:3981590/1‑93 (MQ=255)
                                                                      ttCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGGCCTTTCGt                   <  1:6468760/93‑1 (MQ=255)
                                                                        cATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCTGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCa                 >  2:68388/1‑93 (MQ=255)
                                                                           aaaGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCaaaa              <  2:1796691/93‑1 (MQ=255)
                                                                               ccccTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTc          <  1:5720076/93‑1 (MQ=255)
                                                                                cccTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCt         >  2:2527671/1‑93 (MQ=255)
                                                                                 ccTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTg        >  2:5935772/1‑93 (MQ=255)
                                                                                  cTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGc       <  2:5671025/93‑1 (MQ=255)
                                                                                   ttttCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCg      >  2:5825873/1‑93 (MQ=255)
                                                                                   ttttCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCg      >  2:5204305/1‑93 (MQ=255)
                                                                                      tCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTaa   <  2:3414674/93‑1 (MQ=255)
                                                                                      tCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTaa   >  2:2782938/1‑93 (MQ=255)
                                                                                       ccAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTaaa  >  2:5269428/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAA  >  NC_000913/3928579‑3928758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGCGGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGC  >  NC_000913/3928576‑3928762
                                                                                             |                                                                                             
CTGGCGGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAG                                                                                               <  SRR4302547.4588616/94‑1 (MQ=60)
  GGCGGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCG                                                                                             <  SRR4302547.1145937/94‑1 (MQ=60)
  GGCGGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCG                                                                                             <  SRR4302547.448464/94‑1 (MQ=60)
     GGGCCAAGGAGGAACACACTTTTACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCTGCTTTTCCAGCGAAC                                                                                          >  SRR4302547.2839190/1‑94 (MQ=60)
     GGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAAC                                                                                          >  SRR4302547.5112872/1‑94 (MQ=60)
     GGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAAC                                                                                          >  SRR4302547.365851/1‑94 (MQ=60)
      GGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACT                                                                                         <  SRR4302547.1683866/94‑1 (MQ=60)
        CCAAGGAGGAACACACTTTCCCCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATTAAGCCCCTTTTCCAGCGAACTGC                                                                                       >  SRR4302547.3836839/1‑94 (MQ=60)
        CCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGC                                                                                       >  SRR4302547.6074736/1‑94 (MQ=60)
         CAAGGAGGACCACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCT                                                                                      <  SRR4302547.4277559/94‑1 (MQ=60)
         CAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCT                                                                                      <  SRR4302547.756713/94‑1 (MQ=60)
         CAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCT                                                                                      >  SRR4302547.6722800/1‑94 (MQ=60)
          AAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTC                                                                                     <  SRR4302547.3777471/94‑1 (MQ=60)
          AAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTC                                                                                     <  SRR4302547.6624027/94‑1 (MQ=60)
           AGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCA                                                                                    >  SRR4302547.509012/1‑94 (MQ=60)
            GGAGGAACACGCTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAG                                                                                   <  SRR4302547.1783754/94‑1 (MQ=60)
            GGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAG                                                                                   <  SRR4302547.1072938/94‑1 (MQ=60)
             GAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGG                                                                                  <  SRR4302547.4921176/94‑1 (MQ=60)
             GAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGG                                                                                  <  SRR4302547.5017019/94‑1 (MQ=60)
              AGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGC                                                                                 <  SRR4302547.1335795/94‑1 (MQ=60)
              AGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGC                                                                                 >  SRR4302547.2253166/1‑94 (MQ=60)
                GAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGG                                                                               >  SRR4302547.4858215/1‑94 (MQ=60)
                 AACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGG                                                                              <  SRR4302547.6259803/94‑1 (MQ=60)
                 AACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGG                                                                              >  SRR4302547.2100048/1‑94 (MQ=60)
                 AACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCCCAGGCGGG                                                                              <  SRR4302547.6674576/94‑1 (MQ=60)
                  ACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGA                                                                             >  SRR4302547.5325534/1‑94 (MQ=60)
                     CACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAAT                                                                          <  SRR4302547.6718125/94‑1 (MQ=60)
                     CACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAAT                                                                          <  SRR4302547.2633826/94‑1 (MQ=60)
                     CACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAAT                                                                          >  SRR4302547.5338082/1‑94 (MQ=60)
                      ACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATT                                                                         <  SRR4302547.2736228/94‑1 (MQ=60)
                      ACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATT                                                                         >  SRR4302547.2337409/1‑94 (MQ=60)
                      ACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATT                                                                         <  SRR4302547.1500939/94‑1 (MQ=60)
                       CTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTC                                                                        <  SRR4302547.5872097/94‑1 (MQ=60)
                       CTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTC                                                                        >  SRR4302547.1923619/1‑94 (MQ=60)
                        TTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCT                                                                       <  SRR4302547.4835771/94‑1 (MQ=60)
                         TTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTT                                                                      >  SRR4302547.4100022/1‑94 (MQ=60)
                          TCTCCACTTAATGCAGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTT                                                                     <  SRR4302547.4702514/94‑1 (MQ=60)
                          TCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTT                                                                     <  SRR4302547.4924858/94‑1 (MQ=60)
                          TCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTT                                                                     <  SRR4302547.2555882/94‑1 (MQ=60)
                          TCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTT                                                                     >  SRR4302547.2439507/1‑94 (MQ=60)
                           CACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTC                                                                    <  SRR4302547.5608690/94‑1 (MQ=60)
                           CACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTC                                                                    <  SRR4302547.6072809/94‑1 (MQ=60)
                           CACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGACCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTC                                                                    >  SRR4302547.2568643/1‑94 (MQ=60)
                            CCCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCC                                                                   <  SRR4302547.4024955/94‑1 (MQ=60)
                            ACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCC                                                                   <  SRR4302547.3390100/94‑1 (MQ=60)
                            ACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCC                                                                   >  SRR4302547.1177811/1‑94 (MQ=60)
                             CCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCG                                                                  >  SRR4302547.2601857/1‑94 (MQ=60)
                             CCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCG                                                                  >  SRR4302547.5300022/1‑94 (MQ=60)
                              CACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGC                                                                 <  SRR4302547.565455/94‑1 (MQ=60)
                                CTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTA                                                               <  SRR4302547.2705984/94‑1 (MQ=60)
                                CTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTA                                                               <  SRR4302547.3509101/94‑1 (MQ=60)
                                 TTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAA                                                              >  SRR4302547.926920/1‑94 (MQ=60)
                                 TTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAA                                                              >  SRR4302547.4401715/1‑94 (MQ=60)
                                 TTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAA                                                              >  SRR4302547.3663149/1‑94 (MQ=60)
                                  TAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAAT                                                             <  SRR4302547.1150521/94‑1 (MQ=60)
                                  TAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAAT                                                             >  SRR4302547.6562433/1‑94 (MQ=60)
                                   AATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATA                                                            >  SRR4302547.6767584/1‑94 (MQ=60)
                                     TGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAA                                                          <  SRR4302547.1042911/94‑1 (MQ=60)
                                     TGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAA                                                          >  SRR4302547.2875560/1‑94 (MQ=60)
                                       CCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGCAATTCTTTCCGCTAATAAAat                                                        >  SRR4302547.5269559/1‑92 (MQ=60)
                                       CCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATG                                                        >  SRR4302547.3318834/1‑94 (MQ=60)
                                        CGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGA                                                       >  SRR4302547.3416162/1‑94 (MQ=60)
                                         GCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAG                                                      >  SRR4302547.1870991/1‑94 (MQ=60)
                                          CTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGG                                                     >  SRR4302547.4295026/1‑94 (MQ=60)
                                          CTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGG                                                     <  SRR4302547.1819284/94‑1 (MQ=60)
                                          CTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGG                                                     >  SRR4302547.497569/1‑94 (MQ=60)
                                          CTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAAG                                                     >  SRR4302547.4384872/1‑94 (MQ=60)
                                           TAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGG                                                    >  SRR4302547.104412/1‑94 (MQ=60)
                                            AATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGT                                                   <  SRR4302547.3661938/94‑1 (MQ=60)
                                            AATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGT                                                   >  SRR4302547.6751728/1‑94 (MQ=60)
                                            AATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGG                                                   >  SRR4302547.6357958/1‑94 (MQ=60)
                                              TAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGA                                                 >  SRR4302547.2013119/1‑94 (MQ=60)
                                              TAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGA                                                 >  SRR4302547.5089219/1‑94 (MQ=60)
                                               AAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAG                                                >  SRR4302547.6509786/1‑94 (MQ=60)
                                               AAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAG                                                <  SRR4302547.5769041/94‑1 (MQ=60)
                                                AACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTTCGCTAATAATTGAGGGTGAGC                                               >  SRR4302547.2777267/1‑94 (MQ=60)
                                                 ACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCTCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCC                                              <  SRR4302547.3286938/94‑1 (MQ=60)
                                                  CACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCA                                             >  SRR4302547.4652452/1‑94 (MQ=60)
                                                  CACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCA                                             >  SRR4302547.3444210/1‑94 (MQ=60)
                                                  CACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCA                                             >  SRR4302547.1412794/1‑94 (MQ=60)
                                                   ACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCAT                                            >  SRR4302547.4909892/1‑94 (MQ=60)
                                                   ACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCAT                                            <  SRR4302547.6435813/94‑1 (MQ=60)
                                                   ACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCAT                                            >  SRR4302547.5731593/1‑94 (MQ=60)
                                                   ACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCAT                                            >  SRR4302547.2280404/1‑94 (MQ=60)
                                                    CAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATA                                           >  SRR4302547.3747455/1‑94 (MQ=60)
                                                     AAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAA                                          <  SRR4302547.1305172/94‑1 (MQ=60)
                                                      AGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAAT                                         <  SRR4302547.5751005/94‑1 (MQ=60)
                                                       GCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATG                                        <  SRR4302547.2779318/94‑1 (MQ=60)
                                                        CGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGA                                       <  SRR4302547.278840/94‑1 (MQ=60)
                                                        CGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGA                                       <  SRR4302547.2836418/94‑1 (MQ=60)
                                                         GGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAA                                      >  SRR4302547.617833/1‑94 (MQ=60)
                                                          GATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAG                                     >  SRR4302547.136712/1‑94 (MQ=60)
                                                          GATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAG                                     >  SRR4302547.3462621/1‑94 (MQ=60)
                                                           ATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGT                                    >  SRR4302547.4569690/1‑94 (MQ=60)
                                                           ATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGGGAGCCATAATGAAGT                                    >  SRR4302547.3554149/1‑94 (MQ=60)
                                                            TGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTG                                   <  SRR4302547.5614226/94‑1 (MQ=60)
                                                            TGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTG                                   >  SRR4302547.4931387/1‑94 (MQ=60)
                                                            TGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTG                                   <  SRR4302547.5195713/94‑1 (MQ=60)
                                                             GGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGG                                  >  SRR4302547.1886110/1‑94 (MQ=60)
                                                             GGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGG                                  >  SRR4302547.3596331/1‑94 (MQ=60)
                                                             GGCGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGG                                  >  SRR4302547.1594761/1‑94 (MQ=60)
                                                               CGTGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCG                                >  SRR4302547.895570/1‑94 (MQ=60)
                                                                 TGGCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTC                              >  SRR4302547.5061936/1‑94 (MQ=60)
                                                                   GCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCT                            <  SRR4302547.6473017/94‑1 (MQ=60)
                                                                   GCTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCT                            <  SRR4302547.2369887/94‑1 (MQ=60)
                                                                    CTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTT                           <  SRR4302547.5702557/94‑1 (MQ=60)
                                                                    CTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTT                           >  SRR4302547.3612081/1‑94 (MQ=60)
                                                                    CTACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTT                           <  SRR4302547.203295/94‑1 (MQ=60)
                                                                     TACGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTT                          <  SRR4302547.5142732/94‑1 (MQ=60)
                                                                       CGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCG                        <  SRR4302547.5505934/94‑1 (MQ=60)
                                                                       CGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCG                        <  SRR4302547.6201095/94‑1 (MQ=60)
                                                                       CGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCG                        <  SRR4302547.5997939/94‑1 (MQ=60)
                                                                       CGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCG                        >  SRR4302547.3641805/1‑94 (MQ=60)
                                                                       CGTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCG                        <  SRR4302547.4092459/94‑1 (MQ=60)
                                                                       CGTGCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCG                        <  SRR4302547.2921498/94‑1 (MQ=60)
                                                                        GTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGT                       <  SRR4302547.2346814/94‑1 (MQ=60)
                                                                        GTTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGT                       >  SRR4302547.2998115/1‑94 (MQ=60)
                                                                         TTCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGGCCTTTCGTC                      <  SRR4302547.6603473/94‑1 (MQ=60)
                                                                          TCATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCA                     >  SRR4302547.5067632/1‑94 (MQ=60)
                                                                           CATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAA                    >  SRR4302547.2831171/1‑94 (MQ=60)
                                                                           CATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAA                    >  SRR4302547.5040557/1‑94 (MQ=60)
                                                                            ATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAA                   >  SRR4302547.1152015/1‑94 (MQ=60)
                                                                            ATAAAGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAA                   >  SRR4302547.703066/1‑94 (MQ=60)
                                                                                AGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTT               >  SRR4302547.6014346/1‑94 (MQ=60)
                                                                                AGCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTT               >  SRR4302547.1103328/1‑94 (MQ=60)
                                                                                 GCCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTC              >  SRR4302547.776980/1‑94 (MQ=60)
                                                                                  CCCCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCT             <  SRR4302547.5838235/94‑1 (MQ=60)
                                                                                    CCTTTTCCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGC           >  SRR4302547.3371890/1‑94 (MQ=60)
                                                                                          CCAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAAT     <  SRR4302547.5413743/94‑1 (MQ=60)
                                                                                           CAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATT    >  SRR4302547.1612347/1‑94 (MQ=60)
                                                                                           CAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATT    >  SRR4302547.5748340/1‑94 (MQ=60)
                                                                                           CAGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATT    <  SRR4302547.4533731/94‑1 (MQ=60)
                                                                                           CAGCGAACTGCTCAGGCGGAAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATT    <  SRR4302547.1784206/94‑1 (MQ=60)
                                                                                            AGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGGGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTG   <  SRR4302547.423713/94‑1 (MQ=60)
                                                                                            AGCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTG   <  SRR4302547.1711689/94‑1 (MQ=60)
                                                                                             GCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGC  <  SRR4302547.508178/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGGCGGGCCAAGGAGGAACACACTTTCACCACTTAATGCCGCTAATAAACACAAGCGGATGGCGTGGCTACGTTCATAAAGCCCCTTTTCCAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGC  >  NC_000913/3928576‑3928762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑