Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,965,750	T→C	S74P (TCT→CCT)	rfe →	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,965,750	0	T	C	87.7%	611.2 / 67.0	220	S74P (TCT→CCT)	rfe	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand): ref base T (15/12); new base C (102/91); total (117/103)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

Only 100 of 259 total aligned reads displayed.

ACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACA  >  NC_000913/3965658‑3965841

  cAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcct                                                                                           <  1:4240469/93‑1 (MQ=255)

  cAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcct                                                                                           <  1:2707198/93‑1 (MQ=255)

  cAGGGATTGATACCTCTCGTTGGGGCTATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcct                                                                                           <  1:5118378/93‑1 (MQ=255)

   aGGGATTGATCCCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcctc                                                                                          <  1:6569941/93‑1 (MQ=255)

   aGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAtctc                                                                                          <  1:5767407/93‑1 (MQ=255)

   aGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcctc                                                                                          <  2:3177309/93‑1 (MQ=255)

    gggATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGCTTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAtctct                                                                                         >  1:2765216/1‑93 (MQ=255)

    gggATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcctct                                                                                         >  1:3919427/1‑93 (MQ=255)

    gggATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcctct                                                                                         >  1:2153126/1‑93 (MQ=255)

     ggATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAtctctc                                                                                        <  1:6210800/93‑1 (MQ=255)

     ggATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAcctctc                                                                                        <  2:358185/93‑1 (MQ=255)

      gATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATctctct                                                                                       >  2:3450798/1‑93 (MQ=255)

      gATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATctctct                                                                                       <  2:5696307/93‑1 (MQ=255)

        ttGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTAt                                                                                     >  1:3229225/1‑93 (MQ=255)

         tGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTAtc                                                                                    <  1:4418406/93‑1 (MQ=255)

          gATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTAtct                                                                                   <  2:2769517/93‑1 (MQ=255)

           aTACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTAtctc                                                                                  >  1:4161906/1‑93 (MQ=255)

            tACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCg                                                                                 >  1:5644793/1‑93 (MQ=255)

             tccTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGc                                                                                <  2:2959623/92‑1 (MQ=255)

              ccTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCt                                                                               <  2:5174774/93‑1 (MQ=255)

              ccTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCt                                                                               >  1:2839234/1‑93 (MQ=255)

              ccTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCt                                                                               >  2:5594321/1‑93 (MQ=255)

                tctcGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTtg                                                                             <  1:1953690/93‑1 (MQ=255)

                tctcGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTtg                                                                             <  2:1397377/93‑1 (MQ=255)

                 ctcGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTtgt                                                                            <  2:5285941/93‑1 (MQ=255)

                   cGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTAAATTCCGCATGCACCTCTCTATCTCGCTTGTGc                                                                          <  1:4215341/93‑1 (MQ=255)

                    gTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGcc                                                                         >  2:4521990/1‑93 (MQ=255)

                    gTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGcc                                                                         <  2:5951497/93‑1 (MQ=255)

                     gtGGGGGGATTTCGGTTTACGCAGGTATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCg                                                                        <  1:2920958/92‑1 (MQ=255)

                      tGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCgg                                                                       >  1:3245081/1‑93 (MQ=255)

                       ggggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGgt                                                                      <  1:5975978/93‑1 (MQ=255)

                       ggggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGgt                                                                      >  2:2545684/1‑93 (MQ=255)

                        gggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGgtg                                                                     <  1:3988656/93‑1 (MQ=255)

                        gggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGgtg                                                                     >  1:3278710/1‑93 (MQ=255)

                        gggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGgtg                                                                     <  2:1769054/93‑1 (MQ=255)

                        gggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGgtg                                                                     >  2:6611100/1‑93 (MQ=255)

                         ggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGgtgt                                                                    <  2:1058164/93‑1 (MQ=255)

                         ggggATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGgtgt                                                                    >  1:6306397/1‑93 (MQ=255)

                            gATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCt                                                                 >  2:3383484/1‑93 (MQ=255)

                            gATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCt                                                                 <  2:1432885/93‑1 (MQ=255)

                            gATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCt                                                                 >  2:858453/1‑93 (MQ=255)

                             aTTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCtt                                                                <  2:15671/93‑1 (MQ=255)

                              tttCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTg                                                               >  2:5499261/1‑93 (MQ=255)

                                tCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGtt                                                             <  2:1113505/93‑1 (MQ=255)

                                 cGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGttt                                                            <  2:5771467/93‑1 (MQ=255)

                                   gTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTc                                                          >  1:5017961/1‑93 (MQ=255)

                                      tACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCAtt                                                       <  1:4647857/93‑1 (MQ=255)

                                      tACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCAtt                                                       <  2:3687236/93‑1 (MQ=255)

                                       aCGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTg                                                      >  2:396168/1‑93 (MQ=255)

                                       aCGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTg                                                      >  1:4540480/1‑93 (MQ=255)

                                         gCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgc                                                    <  1:3536631/93‑1 (MQ=255)

                                         gCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgc                                                    <  2:3641251/93‑1 (MQ=255)

                                         gCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgc                                                    <  1:6625262/93‑1 (MQ=255)

                                          cAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgcg                                                   <  2:725182/93‑1 (MQ=255)

                                           aGGGATTTGCTTCACGTTCGGGATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTCCTTGTTTTCATTAgcgg                                                  >  1:1538553/1‑92 (MQ=255)

                                           aGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgcgc                                                  >  1:1303627/1‑93 (MQ=255)

                                           aGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgcgt                                                  >  1:1549695/1‑92 (MQ=255)

                                           aGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgcgc                                                  <  2:1783280/93‑1 (MQ=255)

                                            gggATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgcgcg                                                 >  2:3730950/1‑93 (MQ=255)

                                             ggATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgcgcgc                                                >  2:457723/1‑93 (MQ=255)

                                             ggATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGgcgcgc                                                >  1:6149232/1‑93 (MQ=255)

                                               aTTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTg                                              <  2:5644487/93‑1 (MQ=255)

                                                tttGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTgg                                             <  1:3741235/93‑1 (MQ=255)

                                                 ttGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGa                                            <  1:1776027/93‑1 (MQ=255)

                                                  tGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGAt                                           >  1:1159706/1‑93 (MQ=255)

                                                  tGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGAt                                           <  2:4707239/93‑1 (MQ=255)

                                                  tGCTTCACGTTCGGAATTGGCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGAt                                           >  1:6417075/1‑93 (MQ=255)

                                                   gCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATg                                          <  2:4692506/93‑1 (MQ=255)

                                                    cTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGa                                         <  1:3089702/93‑1 (MQ=255)

                                                    cTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGa                                         <  2:5818421/93‑1 (MQ=255)

                                                         cGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGtt                                    >  2:4047319/1‑93 (MQ=255)

                                                          gTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGttt                                   >  1:893033/1‑93 (MQ=255)

                                                          gTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGttt                                   >  1:3867898/1‑93 (MQ=255)

                                                           ttCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGtttt                                  <  2:2375265/93‑1 (MQ=255)

                                                           ttCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGtttt                                  <  1:4849861/93‑1 (MQ=255)

                                                              ggAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGat                               >  1:244068/1‑93 (MQ=255)

                                                               gAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGata                              >  2:4159135/1‑93 (MQ=255)

                                                                 aTTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATc                            >  1:84897/1‑93 (MQ=255)

                                                                 aTTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATc                            >  1:5921549/1‑93 (MQ=255)

                                                                  ttGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCa                           >  2:2273918/1‑93 (MQ=255)

                                                                  ttGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCa                           >  2:5436272/1‑93 (MQ=255)

                                                                  ttGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCa                           <  2:3343164/93‑1 (MQ=255)

                                                                    gTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGc                         <  1:928419/93‑1 (MQ=255)

                                                                      cGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGt                       >  2:4952276/1‑93 (MQ=255)

                                                                        aTTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTaa                     >  2:2156230/1‑93 (MQ=255)

                                                                         ttACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTaaa                    >  2:705931/1‑93 (MQ=255)

                                                                         tgaCTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTaaa                    >  1:5277471/3‑93 (MQ=255)

                                                                          tACTATATTCCGCATGCACTTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTaaaa                   >  1:6462667/1‑93 (MQ=255)

                                                                           aCTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTaaaaa                  <  1:2516584/93‑1 (MQ=255)

                                                                             tataTCCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTTATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATc                <  1:2432872/93‑1 (MQ=255)

                                                                              atatTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATcc               >  1:5481438/1‑93 (MQ=255)

                                                                              atatTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATcc               <  1:3671081/93‑1 (MQ=255)

                                                                               tatTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCg              >  2:6521492/1‑93 (MQ=255)

                                                                                atTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGt             <  1:5520208/93‑1 (MQ=255)

                                                                                   ccGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGcc          >  1:1085858/1‑93 (MQ=255)

                                                                                    cGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGcca         >  2:1965879/1‑93 (MQ=255)

                                                                                       atgcacCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGccacca      >  1:5775079/1‑93 (MQ=255)

                                                                                        tgcacCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCAt     >  2:6007429/1‑93 (MQ=255)

                                                                                        tgcacCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCAt     >  2:5119613/1‑93 (MQ=255)

                                                                                        tgcacCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCAt     >  1:1193785/1‑93 (MQ=255)

ACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACA  >  NC_000913/3965658‑3965841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGG  >  NC_000913/3965659‑3965843

CCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCT                                                                                             <  SRR4302547.4215894/94‑1 (MQ=60)

CCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCT                                                                                             >  SRR4302547.1326417/1‑94 (MQ=60)

 CAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTC                                                                                            <  SRR4302547.2761558/94‑1 (MQ=60)

 CAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTC                                                                                            <  SRR4302547.4327181/94‑1 (MQ=60)

 CAGGGATTGATACCTCTCGTTGGGGCTATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTC                                                                                            <  SRR4302547.5223834/94‑1 (MQ=60)

  AGGGATTGATCCCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCT                                                                                           <  SRR4302547.6706733/94‑1 (MQ=60)

  AGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGCTTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCT                                                                                           >  SRR4302547.2820625/1‑94 (MQ=60)

  AGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCT                                                                                           <  SRR4302547.5886682/94‑1 (MQ=60)

  AGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCT                                                                                           <  SRR4302547.4895189/94‑1 (MQ=60)

  AGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCT                                                                                           >  SRR4302547.2196120/1‑94 (MQ=60)

  AGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCT                                                                                           >  SRR4302547.3999124/1‑94 (MQ=60)

    GGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCT                                                                                         <  SRR4302547.6339770/94‑1 (MQ=60)

    GGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCT                                                                                         <  SRR4302547.6348785/94‑1 (MQ=60)

     GATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTA                                                                                        <  SRR4302547.3241706/94‑1 (MQ=60)

      ATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTAT                                                                                       >  SRR4302547.3293671/1‑94 (MQ=60)

      ATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTAT                                                                                       >  SRR4302547.5950982/1‑94 (MQ=60)

        TGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCT                                                                                     <  SRR4302547.4509593/94‑1 (MQ=60)

         GATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTC                                                                                    >  SRR4302547.4246690/1‑94 (MQ=60)

          ATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCG                                                                                   >  SRR4302547.5761309/1‑94 (MQ=60)

          ATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCG                                                                                   >  SRR4302547.1493130/1‑94 (MQ=60)

           TACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGC                                                                                  >  SRR4302547.3982842/1‑94 (MQ=60)

            ACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCT                                                                                 >  SRR4302547.5714253/1‑94 (MQ=60)

            ACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCT                                                                                 >  SRR4302547.2895978/1‑94 (MQ=60)

             CCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTT                                                                                <  SRR4302547.2860243/94‑1 (MQ=60)

              CTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTT                                                                               >  SRR4302547.5573421/1‑94 (MQ=60)

               TCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGCATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGT                                                                              >  SRR4302547.3428774/1‑94 (MQ=60)

               TCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGT                                                                              <  SRR4302547.1992444/94‑1 (MQ=60)

                  CGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCGCCTCTCTATCTCGCTTGTGCC                                                                           <  SRR4302547.3951756/94‑1 (MQ=60)

                  CGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCC                                                                           >  SRR4302547.2365991/1‑94 (MQ=60)

                  CGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTAAATTCCGCATGCACCTCTCTATCTCGCTTGTGCC                                                                           <  SRR4302547.4301392/94‑1 (MQ=60)

                   GTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCG                                                                          <  SRR4302547.1108498/94‑1 (MQ=60)

                    TTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGG                                                                         >  SRR4302547.3309905/1‑94 (MQ=60)

                    GTGGGGGGATTTCGGTTTACGCAGGTATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGG                                                                         <  SRR4302547.2979196/94‑1 (MQ=60)

                      GGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTG                                                                       <  SRR4302547.6099930/94‑1 (MQ=60)

                      GGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTG                                                                       >  SRR4302547.3344439/1‑94 (MQ=60)

                      GGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTG                                                                       >  SRR4302547.296936/1‑94 (MQ=60)

                       GGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGT                                                                      <  SRR4302547.4069757/94‑1 (MQ=60)

                       GGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGT                                                                      >  SRR4302547.6437514/1‑94 (MQ=60)

                       GGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGT                                                                      <  SRR4302547.2880834/94‑1 (MQ=60)

                        GGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTG                                                                     >  SRR4302547.5798476/1‑94 (MQ=60)

                            ATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTG                                                                 <  SRR4302547.4299554/94‑1 (MQ=60)

                                 GGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTC                                                            >  SRR4302547.5121668/1‑94 (MQ=60)

                                  GTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCA                                                           <  SRR4302547.613979/94‑1 (MQ=60)

                                   TTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCAT                                                          <  SRR4302547.3083411/94‑1 (MQ=60)

                                     TACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTG                                                        <  SRR4302547.4744138/94‑1 (MQ=60)

                                     TACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTG                                                        >  SRR4302547.4634342/1‑94 (MQ=60)

                                      ACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGC                                                       <  SRR4302547.2993020/94‑1 (MQ=60)

                                        GCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCG                                                     >  SRR4302547.4242678/1‑94 (MQ=60)

                                        GCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCG                                                     <  SRR4302547.3608837/94‑1 (MQ=60)

                                        GCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCG                                                     <  SRR4302547.6692272/94‑1 (MQ=60)

                                        GCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCG                                                     >  SRR4302547.2377193/1‑94 (MQ=60)

                                        GCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCG                                                     <  SRR4302547.6763181/94‑1 (MQ=60)

                                        GCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCG                                                     <  SRR4302547.6732474/94‑1 (MQ=60)

                                         CAGGGATTTGCTTCACGTTCGGGATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTCCTTGTTTTCATTagcgg                                                    >  SRR4302547.1569182/1‑89 (MQ=60)

                                         CAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGC                                                    >  SRR4302547.1329853/1‑94 (MQ=60)

                                         CAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGT                                                    >  SRR4302547.1580545/1‑94 (MQ=60)

                                         CAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGC                                                    <  SRR4302547.5794265/94‑1 (MQ=60)

                                         CAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGC                                                    >  SRR4302547.3833603/1‑94 (MQ=60)

                                         CAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGC                                                    <  SRR4302547.434276/94‑1 (MQ=60)

                                          AGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCG                                                   >  SRR4302547.3789229/1‑94 (MQ=60)

                                          AGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCG                                                   >  SRR4302547.883005/1‑94 (MQ=60)

                                          AGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCG                                                   >  SRR4302547.4310944/1‑94 (MQ=60)

                                          AGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCG                                                   >  SRR4302547.1952329/1‑94 (MQ=60)

                                          AGGGATTTGCTCCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCG                                                   <  SRR4302547.6095723/94‑1 (MQ=60)

                                           GGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGC                                                  >  SRR4302547.894073/1‑94 (MQ=60)

                                           GGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGC                                                  >  SRR4302547.6276811/1‑94 (MQ=60)

                                             GATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTG                                                >  SRR4302547.5887632/1‑94 (MQ=60)

                                              ATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGG                                               <  SRR4302547.4647352/94‑1 (MQ=60)

                                              ATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGG                                               <  SRR4302547.2733579/94‑1 (MQ=60)

                                               TTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGA                                              <  SRR4302547.3817610/94‑1 (MQ=60)

                                                TTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGAT                                             <  SRR4302547.1811252/94‑1 (MQ=60)

                                                TTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGAT                                             >  SRR4302547.1183163/1‑94 (MQ=60)

                                                TTGCTTCACGTTCGGAATTGGCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGAT                                             >  SRR4302547.6550876/1‑94 (MQ=60)

                                                   CTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGAC                                          <  SRR4302547.3150987/94‑1 (MQ=60)

                                                   CTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGAC                                          <  SRR4302547.2528208/94‑1 (MQ=60)

                                                   CTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGAC                                          >  SRR4302547.3192282/1‑94 (MQ=60)

                                                   CTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGAC                                          >  SRR4302547.1193538/1‑94 (MQ=60)

                                                    TTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACC                                         >  SRR4302547.6135809/1‑94 (MQ=60)

                                                    TTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACC                                         <  SRR4302547.2540647/94‑1 (MQ=60)

                                                     TCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCG                                        <  SRR4302547.723222/94‑1 (MQ=60)

                                                     TCACGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCG                                        <  SRR4302547.461168/94‑1 (MQ=60)

                                                        CGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTT                                     >  SRR4302547.910205/1‑94 (MQ=60)

                                                        CGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTT                                     >  SRR4302547.3946648/1‑94 (MQ=60)

                                                        CGTTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTT                                     <  SRR4302547.2786940/94‑1 (MQ=60)

                                                        CGTTCCGAATTTTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTT                                     <  SRR4302547.5610107/94‑1 (MQ=60)

                                                         GTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTT                                    <  SRR4302547.4018628/94‑1 (MQ=60)

                                                          TTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTG                                   >  SRR4302547.465311/1‑94 (MQ=60)

                                                          TTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTG                                   >  SRR4302547.3324193/1‑94 (MQ=60)

                                                          TTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTG                                   <  SRR4302547.4950365/94‑1 (MQ=60)

                                                          TTCGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTG                                   >  SRR4302547.5384686/1‑94 (MQ=60)

                                                            CGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGAT                                 >  SRR4302547.1210999/1‑94 (MQ=60)

                                                            CGGAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGAT                                 >  SRR4302547.248624/1‑94 (MQ=60)

                                                              GAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCCTTTTGAAAT                               >  SRR4302547.4307903/1‑94 (MQ=60)

                                                              GAATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATAT                               >  SRR4302547.5713325/1‑94 (MQ=60)

                                                               AATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATC                              >  SRR4302547.6502596/1‑94 (MQ=60)

                                                               AATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATC                              <  SRR4302547.4961903/94‑1 (MQ=60)

                                                               AATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATC                              >  SRR4302547.6044284/1‑94 (MQ=60)

                                                               AATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATC                              >  SRR4302547.86469/1‑94 (MQ=60)

                                                               AATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATC                              >  SRR4302547.171947/1‑94 (MQ=60)

                                                                ATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCA                             >  SRR4302547.248201/1‑94 (MQ=60)

                                                                ATTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCA                             >  SRR4302547.2574433/1‑94 (MQ=60)

                                                                ATTGTCGATTACTAGATTCCGCATGCATCTCTCTATCTCGCTTGTCCCGGTGTGCTTGTTTTCATTGGCGCGCTGTATGACCGTTTTGATATCA                             <  SRR4302547.3727519/94‑1 (MQ=60)

                                                                 TTGTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAG                            <  SRR4302547.2082281/94‑1 (MQ=60)

                                                                  TGCCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGCCCGTTTTGATATCGGC                           >  SRR4302547.1397093/1‑94 (MQ=60)

                                                                   GTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCG                          >  SRR4302547.5984630/1‑94 (MQ=60)

                                                                   GTCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCG                          <  SRR4302547.946356/94‑1 (MQ=60)

                                                                    TCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGT                         >  SRR4302547.3334172/1‑94 (MQ=60)

                                                                    TCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGT                         >  SRR4302547.3304865/1‑94 (MQ=60)

                                                                    TCGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGT                         <  SRR4302547.6542894/94‑1 (MQ=60)

                                                                     CGATTACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTA                        >  SRR4302547.857232/1‑94 (MQ=60)

                                                                       ATGACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAA                      >  SRR4302547.5386301/1‑94 (MQ=60)

                                                                        TTACTATATTCCGCATGCACTTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAA                     >  SRR4302547.6597245/1‑94 (MQ=60)

                                                                          ACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAAT                   >  SRR4302547.6589687/1‑94 (MQ=60)

                                                                          ACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAAT                   >  SRR4302547.5223733/1‑94 (MQ=60)

                                                                          ACTATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAAT                   <  SRR4302547.2567456/94‑1 (MQ=60)

                                                                           CTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATC                  >  SRR4302547.6704799/1‑94 (MQ=60)

                                                                            TATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCC                 >  SRR4302547.5594626/1‑94 (MQ=60)

                                                                            TATATCCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTTATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCC                 <  SRR4302547.2482091/94‑1 (MQ=60)

                                                                             ATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCG                <  SRR4302547.936255/94‑1 (MQ=60)

                                                                             ATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCG                <  SRR4302547.3745954/94‑1 (MQ=60)

                                                                             ATATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATCGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCG                <  SRR4302547.5512332/94‑1 (MQ=60)

                                                                              TATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGT               >  SRR4302547.1625447/1‑94 (MQ=60)

                                                                               ATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTG              >  SRR4302547.6313711/1‑94 (MQ=60)

                                                                               ATTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTG              <  SRR4302547.5634161/94‑1 (MQ=60)

                                                                                TTCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGC             >  SRR4302547.3851416/1‑94 (MQ=60)

                                                                                 TCCGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCC            >  SRR4302547.1107557/1‑94 (MQ=60)

                                                                                   CGCATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCAC          <  SRR4302547.4950976/94‑1 (MQ=60)

                                                                                     CATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCA        >  SRR4302547.6403861/1‑94 (MQ=60)

                                                                                     CATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCA        <  SRR4302547.5153950/94‑1 (MQ=60)

                                                                                     CATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCA        >  SRR4302547.5894527/1‑94 (MQ=60)

                                                                                      ATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCAT       >  SRR4302547.1217878/1‑94 (MQ=60)

                                                                                      ATGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCAT       >  SRR4302547.4352788/1‑94 (MQ=60)

                                                                                       TGCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATA      >  SRR4302547.1577809/1‑94 (MQ=60)

                                                                                        GCACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATAC     <  SRR4302547.3659830/94‑1 (MQ=60)

                                                                                          ATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAG   <  SRR4302547.182828/94‑1 (MQ=60)

                                                                                          ACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAG   <  SRR4302547.3758220/94‑1 (MQ=60)

                                                                                          ACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAG   <  SRR4302547.2228678/94‑1 (MQ=60)

                                                                                          ACCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAG   >  SRR4302547.4005106/1‑94 (MQ=60)

                                                                                          ACCTCTCTATCTCGCTTGTGCCGGGGTGCTTGATTTCATTGGTGCGCTGGATGACCGTTTTGATATCAGCGTCAAAATCCGTGCCACCATACAG   >  SRR4302547.1390596/1‑94 (MQ=60)

                                                                                           CCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGG  >  SRR4302547.3396530/1‑94 (MQ=60)

                                                                                           CCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGG  >  SRR4302547.5099778/1‑94 (MQ=60)

CCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGG  >  NC_000913/3965659‑3965843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`