Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,996,531 C→T G312G (GGC→GGT)  uvrD → DNA‑dependent ATPase I and helicase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,996,5310CT88.8% 751.5 / 62.1 249G312G (GGC→GGT) uvrDDNA‑dependent ATPase I and helicase II
Reads supporting (aligned to +/- strand):  ref base C (12/16);  new base T (112/109);  total (124/125)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

Only 100 of 256 total aligned reads displayed.

AACTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGA  >  NC_000913/3996439‑3996620
                                                                                            |                                                                                         
aaCTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGgc                                                                                           >  2:1542600/1‑93 (MQ=255)
  cTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGgtgc                                                                                         >  1:2257706/1‑93 (MQ=255)
   tACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGcgcg                                                                                        >  1:1547806/1‑93 (MQ=255)
    aCCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCgg                                                                                       >  1:3990307/1‑93 (MQ=255)
     ccGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGa                                                                                      <  1:581722/93‑1 (MQ=255)
        ctctACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACgg                                                                                   <  1:1003397/93‑1 (MQ=255)
         tctACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGt                                                                                  <  1:5705238/93‑1 (MQ=255)
          ctACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTg                                                                                 <  2:6482245/93‑1 (MQ=255)
          ctACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTg                                                                                 <  1:2732978/93‑1 (MQ=255)
           tACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGa                                                                                <  1:6455911/93‑1 (MQ=255)
           tACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGa                                                                                <  2:1696774/93‑1 (MQ=255)
           tACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGa                                                                                <  2:2092265/93‑1 (MQ=255)
             ccAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACACTAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGc                                                                              >  2:4670181/1‑93 (MQ=255)
              cAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGcc                                                                             >  2:709111/1‑93 (MQ=255)
               aGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCt                                                                            >  2:1144071/1‑93 (MQ=255)
                gCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTa                                                                           <  1:2948449/93‑1 (MQ=255)
                gCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTa                                                                           <  2:5241754/93‑1 (MQ=255)
                gCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTa                                                                           >  1:3881059/1‑93 (MQ=255)
                  aaTATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTAtt                                                                         >  2:5425727/1‑93 (MQ=255)
                  aaTATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTAtt                                                                         <  2:2596851/93‑1 (MQ=255)
                  aaTATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTAtt                                                                         <  1:4244030/93‑1 (MQ=255)
                      ttCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTccc                                                                     >  1:6642394/1‑93 (MQ=255)
                       tCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCct                                                                    <  2:5498536/93‑1 (MQ=255)
                        cTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCctc                                                                   <  2:2464586/93‑1 (MQ=255)
                         tGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCctct                                                                  >  1:835160/1‑93 (MQ=255)
                         tGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCctct                                                                  >  1:6168545/1‑93 (MQ=255)
                         tGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCctct                                                                  >  1:3366116/1‑93 (MQ=255)
                            gcgcCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTAtt                                                               <  1:3376248/93‑1 (MQ=255)
                            gcgcCGCTAACGCCCTGATTGAAAACAATAACGGGCCTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTAtt                                                               <  2:2013140/93‑1 (MQ=255)
                                 gcTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCt                                                          >  2:4390995/1‑93 (MQ=255)
                                 gcTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCt                                                          >  2:647108/1‑93 (MQ=255)
                                 gcTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCt                                                          <  1:3001260/93‑1 (MQ=255)
                                  cTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCtt                                                         >  1:3664479/1‑93 (MQ=255)
                                    aaCGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCtttt                                                       >  2:6506692/1‑93 (MQ=255)
                                    aaCGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCtttt                                                       <  1:2555326/93‑1 (MQ=255)
                                    aaCGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCtttt                                                       <  2:2937442/93‑1 (MQ=255)
                                     aCGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTa                                                      <  1:5986066/93‑1 (MQ=255)
                                       gCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAc                                                    >  1:5538766/1‑93 (MQ=255)
                                       gCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAc                                                    >  1:1074409/1‑93 (MQ=255)
                                       gCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAc                                                    <  2:5640790/93‑1 (MQ=255)
                                       gCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAc                                                    >  1:1821199/1‑93 (MQ=255)
                                       gCCCTGATTGAAAACAATAACAGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAc                                                    >  2:354750/1‑93 (MQ=255)
                                        cccTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACg                                                   >  2:5513784/1‑93 (MQ=255)
                                          cTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGaa                                                 >  2:2293914/1‑93 (MQ=255)
                                          cTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGaa                                                 <  1:19737/93‑1 (MQ=255)
                                           tGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAAc                                                <  1:5287168/93‑1 (MQ=255)
                                           tGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAAc                                                >  2:2417631/1‑93 (MQ=255)
                                           tGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAAc                                                >  1:1293883/1‑93 (MQ=255)
                                            gATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGGCGGTGAGCCTATTTCCCTCAATTGCGCTTTTAACGAACt                                               <  1:6440527/93‑1 (MQ=255)
                                             aTTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTc                                              >  2:4707618/1‑93 (MQ=255)
                                              ttGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCg                                             >  2:923834/1‑93 (MQ=255)
                                              ttGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCg                                             >  1:5189938/1‑93 (MQ=255)
                                               tGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGa                                            <  1:2349961/93‑1 (MQ=255)
                                                gAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGAt                                           <  1:6089168/93‑1 (MQ=255)
                                                gAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGAt                                           <  2:5887336/93‑1 (MQ=255)
                                                gAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGAt                                           <  1:4199651/93‑1 (MQ=255)
                                                  aaaCAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGa                                         <  2:3717783/93‑1 (MQ=255)
                                                  aaaCAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGa                                         <  1:5900844/93‑1 (MQ=255)
                                                   aaCAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCTATGaa                                        <  1:4327243/93‑1 (MQ=255)
                                                   aaCAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGaa                                        >  2:6259884/1‑93 (MQ=255)
                                                    aCAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAg                                       >  2:6016837/1‑93 (MQ=255)
                                                    aCAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAg                                       >  1:1667450/1‑93 (MQ=255)
                                                     cAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAgc                                      <  1:2133823/93‑1 (MQ=255)
                                                       aTAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAgcgc                                    <  2:3420427/93‑1 (MQ=255)
                                                       aTAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAgcgc                                    <  1:1496651/93‑1 (MQ=255)
                                                       aTAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAgcgc                                    >  2:1201503/1‑93 (MQ=255)
                                                        tAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGcgcg                                   >  1:4017269/1‑93 (MQ=255)
                                                          aCGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGtt                                 <  1:5225775/93‑1 (MQ=255)
                                                               cGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTtgtg                            <  1:4638227/93‑1 (MQ=255)
                                                               cGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTtgtg                            >  2:3410899/1‑93 (MQ=255)
                                                                gTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTtgtgt                           <  1:6385018/93‑2 (MQ=255)
                                                                gTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTgg                           >  2:5068397/1‑93 (MQ=255)
                                                                gTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTgg                           >  2:1384683/1‑93 (MQ=255)
                                                                 tCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGt                          <  1:3770285/93‑1 (MQ=255)
                                                                     ggTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAAc                      <  2:6333486/93‑1 (MQ=255)
                                                                     ggTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAAc                      <  2:4778448/93‑1 (MQ=255)
                                                                     ggTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAAc                      >  2:5355115/1‑93 (MQ=255)
                                                                      gTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAAcc                     <  1:300084/93‑1 (MQ=255)
                                                                         aaaaaCTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCa                  <  2:6107006/93‑1 (MQ=255)
                                                                          aaaaCTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCAt                 >  1:1221315/1‑93 (MQ=255)
                                                                          aaaaCTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCAt                 <  2:3703133/93‑1 (MQ=255)
                                                                           aaaCTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATc                >  1:954971/1‑93 (MQ=255)
                                                                               tgtgGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCaaaa            <  1:4565541/93‑1 (MQ=255)
                                                                               tgtgGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCaaaa            <  1:4837252/93‑1 (MQ=255)
                                                                                  ggACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCt         <  2:561108/93‑1 (MQ=255)
                                                                                  ggACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCt         <  2:4596132/93‑1 (MQ=255)
                                                                                  ggACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCt         <  1:6318455/93‑1 (MQ=255)
                                                                                  ggACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCt         >  2:1440800/1‑93 (MQ=255)
                                                                                   gACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTg        <  2:4449968/93‑1 (MQ=255)
                                                                                     ccGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGc      <  2:3872720/93‑1 (MQ=255)
                                                                                      cGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCa     >  2:4262023/1‑93 (MQ=255)
                                                                                      cGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCa     <  1:1059408/93‑1 (MQ=255)
                                                                                       gATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCTCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAg    <  1:1323252/93‑1 (MQ=255)
                                                                                       gATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAg    >  2:4986119/1‑93 (MQ=255)
                                                                                       gATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAg    >  2:3963279/1‑93 (MQ=255)
                                                                                       gATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAg    <  1:5424450/93‑1 (MQ=255)
                                                                                         tGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGa  >  2:5824853/1‑93 (MQ=255)
                                                                                         tGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGa  <  2:596423/93‑1 (MQ=255)
                                                                                         tGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGa  >  1:6038119/1‑93 (MQ=255)
                                                                                         tGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGATTTGTGGTTAATCGCATCAAAACCTGGCAGGa  >  1:210056/1‑93 (MQ=255)
                                                                                            |                                                                                         
AACTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGA  >  NC_000913/3996439‑3996620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGACAAC  >  NC_000913/3996440‑3996624
                                                                                           |                                                                                             
ACTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGC                                                                                             >  SRR4302547.2303302/1‑94 (MQ=60)
ACTACCGCTCAACCAGCAATATGCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGC                                                                                             <  SRR4302547.3452147/94‑1 (MQ=60)
 CTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCG                                                                                            <  SRR4302547.1042248/94‑1 (MQ=60)
 CTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCG                                                                                            >  SRR4302547.1578622/1‑94 (MQ=60)
  TACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGG                                                                                           >  SRR4302547.4071452/1‑94 (MQ=60)
   ACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGA                                                                                          >  SRR4302547.1722661/1‑94 (MQ=60)
    CCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGAC                                                                                         <  SRR4302547.592173/94‑1 (MQ=60)
    CCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGAC                                                                                         <  SRR4302547.5216675/94‑1 (MQ=60)
     CGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACG                                                                                        <  SRR4302547.6038118/94‑1 (MQ=60)
     CGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACG                                                                                        >  SRR4302547.5676545/1‑94 (MQ=60)
     CGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACG                                                                                        >  SRR4302547.2875513/1‑94 (MQ=60)
     CGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACG                                                                                        <  SRR4302547.164581/94‑1 (MQ=60)
     CGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACG                                                                                        <  SRR4302547.5926729/94‑1 (MQ=60)
       CTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGT                                                                                      <  SRR4302547.1022971/94‑1 (MQ=60)
       CTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGT                                                                                      <  SRR4302547.3069329/94‑1 (MQ=60)
       CTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGAGGACCGATGGCGCGGACGGT                                                                                      <  SRR4302547.6553023/94‑1 (MQ=60)
        TCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTG                                                                                     <  SRR4302547.1510744/94‑1 (MQ=60)
        TCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTG                                                                                     <  SRR4302547.5823080/94‑1 (MQ=60)
        TCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTG                                                                                     <  SRR4302547.5580215/94‑1 (MQ=60)
         CTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGA                                                                                    <  SRR4302547.4593380/94‑1 (MQ=60)
         CTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGA                                                                                    <  SRR4302547.2787826/94‑1 (MQ=60)
          TACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAG                                                                                   <  SRR4302547.6590378/94‑1 (MQ=60)
             CAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCT                                                                                <  SRR4302547.5434265/94‑1 (MQ=60)
             CAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCT                                                                                >  SRR4302547.2900095/1‑94 (MQ=60)
              AGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTA                                                                               >  SRR4302547.4306657/1‑94 (MQ=60)
              AGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTA                                                                               >  SRR4302547.3960017/1‑94 (MQ=60)
              AGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTA                                                                               >  SRR4302547.615118/1‑94 (MQ=60)
               GCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTAT                                                                              <  SRR4302547.3007171/94‑1 (MQ=60)
               GCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTAT                                                                              >  SRR4302547.1092480/1‑94 (MQ=60)
                 AATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTT                                                                            <  SRR4302547.6187690/94‑1 (MQ=60)
                 AATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTT                                                                            <  SRR4302547.4330827/94‑1 (MQ=60)
                   TATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCC                                                                          <  SRR4302547.2490738/94‑1 (MQ=60)
                    ATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCC                                                                         >  SRR4302547.6780678/1‑94 (MQ=60)
                    ATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCC                                                                         >  SRR4302547.2585890/1‑94 (MQ=60)
                     TTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCT                                                                        <  SRR4302547.6118212/94‑1 (MQ=60)
                       CTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCT                                                                      >  SRR4302547.3434482/1‑94 (MQ=60)
                       CTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCT                                                                      <  SRR4302547.1249820/94‑1 (MQ=60)
                       CTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCT                                                                      >  SRR4302547.6296588/1‑94 (MQ=60)
                       CTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCT                                                                      >  SRR4302547.851101/1‑94 (MQ=60)
                        TGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTT                                                                     >  SRR4302547.6003126/1‑94 (MQ=60)
                        TGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGGCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTCTTTCCCTCTT                                                                     >  SRR4302547.1972350/1‑94 (MQ=60)
                         GAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTAT                                                                    >  SRR4302547.3728527/1‑94 (MQ=60)
                          AGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATT                                                                   >  SRR4302547.1795927/1‑94 (MQ=60)
                          AGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGGGCGGACGGTGAGCCTATTTCCCTCTATT                                                                   >  SRR4302547.5025112/1‑94 (MQ=60)
                           GCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTG                                                                  <  SRR4302547.235163/94‑1 (MQ=60)
                           GCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTG                                                                  <  SRR4302547.3444844/94‑1 (MQ=60)
                             GCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCG                                                                <  SRR4302547.6145872/94‑1 (MQ=60)
                             GCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCG                                                                <  SRR4302547.3048027/94‑1 (MQ=60)
                              CCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGC                                                               <  SRR4302547.4132891/94‑1 (MQ=60)
                                GCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTT                                                             >  SRR4302547.3739231/1‑94 (MQ=60)
                                GCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTT                                                             <  SRR4302547.3060941/94‑1 (MQ=60)
                                GCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTT                                                             <  SRR4302547.3060794/94‑1 (MQ=60)
                                 CTAACGCCCTGATTGAAAACAATAACGTGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCGATTTCCCTCTATTGCGCTTT                                                            <  SRR4302547.6629593/94‑1 (MQ=60)
                                  TAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTT                                                           >  SRR4302547.2147303/1‑94 (MQ=60)
                                  TAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTT                                                           >  SRR4302547.3895283/1‑94 (MQ=60)
                                   AACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTA                                                          <  SRR4302547.2606931/94‑1 (MQ=60)
                                    ACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAA                                                         >  SRR4302547.4700832/1‑94 (MQ=60)
                                    ACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAA                                                         <  SRR4302547.6110241/94‑1 (MQ=60)
                                    ACGCCCTGATTGAAAACAATAACGGGCGTCTGGGGAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAA                                                         >  SRR4302547.4884233/1‑94 (MQ=60)
                                     CGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAC                                                        <  SRR4302547.5497346/94‑1 (MQ=60)
                                     CGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAC                                                        >  SRR4302547.6252949/1‑94 (MQ=60)
                                     CGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAC                                                        >  SRR4302547.1914201/1‑94 (MQ=60)
                                     CGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAC                                                        >  SRR4302547.1095846/1‑94 (MQ=60)
                                     CGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAC                                                        >  SRR4302547.5653087/1‑94 (MQ=60)
                                     CGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAAC                                                        >  SRR4302547.1857202/1‑94 (MQ=60)
                                     CGCCCTGATTGAAAACAAAAACGGGCGGCTGGGTAAAAAACTGTGGACCGATTGCGCGGACGGTGAGCCTTTTTCCCTCCATTGCGCCTTTAAC                                                        <  SRR4302547.387739/94‑1 (MQ=60)
                                      GCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGCTGGTGCGGACGGTGAGCCTAGTTCCCTCTATTGCGCTTTTAACG                                                       >  SRR4302547.6698508/1‑94 (MQ=60)
                                      GCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACG                                                       >  SRR4302547.2233137/1‑94 (MQ=60)
                                       CCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGA                                                      >  SRR4302547.1636791/1‑94 (MQ=60)
                                       CCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTCACGA                                                      >  SRR4302547.1006902/1‑94 (MQ=60)
                                        CCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAA                                                     >  SRR4302547.302633/1‑94 (MQ=60)
                                         CTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAAC                                                    <  SRR4302547.20115/94‑1 (MQ=60)
                                         CTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAAC                                                    >  SRR4302547.1319925/1‑94 (MQ=60)
                                         CTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAAC                                                    >  SRR4302547.2624291/1‑94 (MQ=60)
                                          TGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACT                                                   <  SRR4302547.5396238/94‑1 (MQ=60)
                                           GATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGGCGGTGAGCCTATTTCCCTCAATTGCGCTTTTAACGAACTC                                                  <  SRR4302547.6574756/94‑1 (MQ=60)
                                           GATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTC                                                  <  SRR4302547.6465212/94‑1 (MQ=60)
                                           GATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTC                                                  >  SRR4302547.3972395/1‑94 (MQ=60)
                                            ATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCG                                                 <  SRR4302547.5142131/94‑1 (MQ=60)
                                            ATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTAAACGAACTCG                                                 >  SRR4302547.878168/1‑94 (MQ=60)
                                            ATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCG                                                 >  SRR4302547.5296748/1‑94 (MQ=60)
                                              TGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGAT                                               <  SRR4302547.2397583/94‑1 (MQ=60)
                                               GAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATG                                              <  SRR4302547.6215479/94‑1 (MQ=60)
                                               GAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATG                                              <  SRR4302547.4285306/94‑1 (MQ=60)
                                                AAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGA                                             >  SRR4302547.6003009/1‑94 (MQ=60)
                                                AAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGA                                             <  SRR4302547.2285912/94‑1 (MQ=60)
                                                 AAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAA                                            <  SRR4302547.6023041/94‑1 (MQ=60)
                                                  AACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCTATGAAG                                           <  SRR4302547.4416304/94‑1 (MQ=60)
                                                  AACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAG                                           >  SRR4302547.1700320/1‑94 (MQ=60)
                                                   ACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGC                                          <  SRR4302547.4034746/94‑1 (MQ=60)
                                                    CAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCG                                         <  SRR4302547.2176327/94‑1 (MQ=60)
                                                    CAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCG                                         <  SRR4302547.2103934/94‑1 (MQ=60)
                                                    CAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCG                                         >  SRR4302547.682987/1‑94 (MQ=60)
                                                     AATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGC                                        >  SRR4302547.4732672/1‑94 (MQ=60)
                                                      ATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCG                                       <  SRR4302547.1526508/94‑1 (MQ=60)
                                                      ATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCG                                       >  SRR4302547.4098901/1‑94 (MQ=60)
                                                        AACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTT                                     >  SRR4302547.3739950/1‑94 (MQ=60)
                                                         ACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTT                                    <  SRR4302547.5333340/94‑1 (MQ=60)
                                                         ACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTT                                    >  SRR4302547.1558725/1‑94 (MQ=60)
                                                         ACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTT                                    <  SRR4302547.4195099/94‑1 (MQ=60)
                                                         ACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTT                                    >  SRR4302547.531668/1‑94 (MQ=60)
                                                          CGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTT                                   >  SRR4302547.5323036/1‑94 (MQ=60)
                                                           GGGCGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTG                                  >  SRR4302547.3439792/1‑94 (MQ=60)
                                                              CGTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGG                               <  SRR4302547.4734300/94‑1 (MQ=60)
                                                               GTCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGTT                              <  SRR4302547.6518251/94‑1 (MQ=60)
                                                                TCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTT                             <  SRR4302547.108405/94‑1 (MQ=60)
                                                                TCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTT                             <  SRR4302547.3847168/94‑1 (MQ=60)
                                                                TCTGGGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTT                             >  SRR4302547.6405594/1‑94 (MQ=60)
                                                                    GGTAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACC                         <  SRR4302547.3935349/94‑1 (MQ=60)
                                                                     GTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCG                        <  SRR4302547.305682/94‑1 (MQ=60)
                                                                      TAAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGC                       >  SRR4302547.1439164/1‑94 (MQ=60)
                                                                       AAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCA                      >  SRR4302547.5372003/1‑94 (MQ=60)
                                                                       AAAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCA                      >  SRR4302547.6557919/1‑94 (MQ=60)
                                                                        AAAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCAT                     >  SRR4302547.1245957/1‑94 (MQ=60)
                                                                         AAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATC                    <  SRR4302547.435280/94‑1 (MQ=60)
                                                                         AAAACTGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATC                    >  SRR4302547.973523/1‑94 (MQ=60)
                                                                              TGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAAC               <  SRR4302547.4659997/94‑1 (MQ=60)
                                                                              TGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAAC               <  SRR4302547.4937524/94‑1 (MQ=60)
                                                                              TGTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAAC               >  SRR4302547.6365345/1‑94 (MQ=60)
                                                                               GTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACC              >  SRR4302547.5763362/1‑94 (MQ=60)
                                                                               GTGGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACC              >  SRR4302547.1165802/1‑94 (MQ=60)
                                                                               GTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACC              <  SRR4302547.4367420/94‑1 (MQ=60)
                                                                                TGGACCGATTGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCT             >  SRR4302547.6579270/1‑94 (MQ=60)
                                                                                TGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCT             <  SRR4302547.4087412/94‑1 (MQ=60)
                                                                                 GGACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTG            <  SRR4302547.6449837/94‑1 (MQ=60)
                                                                                 GGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTG            >  SRR4302547.2098654/1‑94 (MQ=60)
                                                                                  GACCGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGG           <  SRR4302547.3256332/94‑1 (MQ=60)
                                                                                     CGATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAG        <  SRR4302547.1080475/94‑1 (MQ=60)
                                                                                      GATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCTCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGG       <  SRR4302547.1349845/94‑1 (MQ=60)
                                                                                      GATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGG       <  SRR4302547.5536633/94‑1 (MQ=60)
                                                                                      GATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGG       >  SRR4302547.3563943/1‑94 (MQ=60)
                                                                                      GATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGG       <  SRR4302547.765341/94‑1 (MQ=60)
                                                                                      GATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGG       <  SRR4302547.1246493/94‑1 (MQ=60)
                                                                                       ATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGA      >  SRR4302547.6163411/1‑94 (MQ=60)
                                                                                       ATGGTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGATTTGTGGTTAATCGCATCAAAACCTGGCAGGA      >  SRR4302547.214015/1‑94 (MQ=60)
                                                                                          GTGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGACAA   <  SRR4302547.978746/94‑1 (MQ=60)
                                                                                           TGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGACAAC  <  SRR4302547.3211124/94‑1 (MQ=60)
                                                                                           TGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGACAAC  <  SRR4302547.846305/94‑1 (MQ=60)
                                                                                           |                                                                                             
ACTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTCTATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGACAAC  >  NC_000913/3996440‑3996624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑