Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,001,486 2 bp→GC coding (316‑317/903 nt) rarD ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,001,4860CG100.0% 946.2 / NA 257R106A (CGG→GCG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (126/131);  total (126/131)
*NC_0009134,001,4870GC100.0% 956.7 / NA 257R106A (CGG→GCG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (126/131);  total (126/131)

Only 100 of 260 total aligned reads displayed.

AGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAA  >  NC_000913/4001399‑4001573
                                                                                       ||                                                                                      
agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                                                                                    <  1:4929158/93‑1 (MQ=255)
agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                                                                                    >  2:4498137/1‑93 (MQ=255)
agccaTCGCATCGGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                                                                                    <  2:3991485/93‑1 (MQ=255)
 gccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                                                                                   >  1:5487255/1‑93 (MQ=255)
 gccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                                                                                   >  1:4524148/1‑93 (MQ=255)
 gccaTTGCATCCGGCGGAATCGCTCGCCGACGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                                                                                   >  2:6195507/1‑93 (MQ=255)
  ccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAg                                                                                  <  2:4280719/93‑1 (MQ=255)
   caTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGc                                                                                 >  2:6464549/1‑93 (MQ=255)
    aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCa                                                                                <  1:6508603/93‑1 (MQ=255)
     ttGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat                                                                               >  1:338630/1‑93 (MQ=255)
     ttGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat                                                                               <  2:4097398/93‑1 (MQ=255)
      tGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCata                                                                              >  1:5656368/1‑93 (MQ=255)
       gCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCatat                                                                             <  1:1971723/93‑1 (MQ=255)
       gCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCatat                                                                             <  1:5128746/93‑1 (MQ=255)
        cATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATg                                                                            <  2:3077863/93‑1 (MQ=255)
         aTCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATgg                                                                           <  2:4909649/93‑1 (MQ=255)
          tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt                                                                          >  1:1720292/1‑93 (MQ=255)
          tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt                                                                          >  2:662702/1‑93 (MQ=255)
            cggcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGa                                                                        >  1:2565405/1‑93 (MQ=255)
            cggcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGa                                                                        <  1:922320/93‑1 (MQ=255)
              gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTGACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAtt                                                                      >  2:4676901/1‑93 (MQ=255)
              gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAtt                                                                      <  2:1353020/93‑1 (MQ=255)
                ggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGt                                                                    <  1:4637698/93‑1 (MQ=255)
                 gAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGtt                                                                   <  1:3719535/93‑1 (MQ=255)
                 gAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGtt                                                                   <  1:5173511/93‑1 (MQ=255)
                    tcgctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCAc                                                                <  2:3597283/89‑1 (MQ=255)
                       ctCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGc                                                             <  2:5146102/92‑1 (MQ=255)
                        tCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGcc                                                            <  2:1691848/93‑1 (MQ=255)
                        tCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGcc                                                            >  2:4441194/1‑93 (MQ=255)
                         cGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGccc                                                           <  2:5875742/93‑1 (MQ=255)
                         cGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGccc                                                           >  2:462298/1‑93 (MQ=255)
                               ggAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATaa                                                     >  1:193713/1‑93 (MQ=255)
                                 aaaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAAc                                                   >  1:5313402/1‑93 (MQ=255)
                                  aaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACa                                                  <  2:4380033/93‑1 (MQ=255)
                                   aaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAg                                                 >  2:3737635/1‑93 (MQ=255)
                                    atcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGt                                                <  1:1025085/93‑1 (MQ=255)
                                    atcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGt                                                <  2:5789659/93‑1 (MQ=255)
                                    atcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGt                                                <  2:2916630/93‑1 (MQ=255)
                                    atcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGt                                                <  1:5866692/93‑1 (MQ=255)
                                     tcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTa                                               <  1:2511031/93‑1 (MQ=255)
                                     tcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTa                                               <  1:3250918/93‑1 (MQ=255)
                                     tcatcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTa                                               <  1:1815638/93‑1 (MQ=255)
                                      catcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAg                                              <  2:2382596/93‑1 (MQ=255)
                                       atcCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGc                                             >  2:3866257/1‑93 (MQ=255)
                                         ccccAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCa                                           >  1:815778/1‑93 (MQ=255)
                                           ccAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGt                                         <  1:778603/93‑1 (MQ=255)
                                           ccAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGt                                         <  2:1146184/93‑1 (MQ=255)
                                             aGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                       >  2:3231853/1‑93 (MQ=255)
                                             aGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                       <  2:3096082/93‑1 (MQ=255)
                                             aGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                       >  2:6290362/1‑93 (MQ=255)
                                             aGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                       >  1:6555640/1‑93 (MQ=255)
                                             aGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                       >  1:157345/1‑93 (MQ=255)
                                              gCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGc                                      >  2:5063490/1‑93 (MQ=255)
                                               caccATGTTCCCCAGCGGGTTTATAATGTAACCAAGGCTCGCTTCCAACATATGGTGATTGTTCACCGCCAAGATAAACAGTAGCCAGGTGcc                                     <  1:2193061/93‑1 (MQ=255)
                                                   aTGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccacca                                 >  1:5940398/1‑93 (MQ=255)
                                                   aTGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccacca                                 <  1:2129965/93‑1 (MQ=255)
                                                   aTGTCCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccacca                                 <  1:1356929/93‑1 (MQ=255)
                                                    tGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCaa                                >  1:6237238/1‑93 (MQ=255)
                                                    tGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCaa                                >  2:771161/1‑93 (MQ=255)
                                                      ttCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATc                              <  1:471958/93‑1 (MQ=255)
                                                      ttCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATc                              >  1:5703624/1‑93 (MQ=255)
                                                         actaGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                           <  2:5416443/90‑1 (MQ=255)
                                                         aCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                           <  2:5456827/93‑1 (MQ=255)
                                                         aCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                           >  1:276787/1‑93 (MQ=255)
                                                         aCCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                           >  2:4784084/1‑93 (MQ=255)
                                                          ccAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCa                          >  1:4045168/1‑93 (MQ=255)
                                                          ccAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCa                          <  1:3688954/93‑1 (MQ=255)
                                                           cAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAc                         <  2:4292295/93‑1 (MQ=255)
                                                           cAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAc                         <  2:5590052/93‑1 (MQ=255)
                                                           cAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAc                         <  1:2868084/93‑1 (MQ=255)
                                                           cAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAc                         <  2:2804588/93‑1 (MQ=255)
                                                           cAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAc                         >  2:5054242/1‑93 (MQ=255)
                                                            aGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACg                        >  1:3418371/1‑93 (MQ=255)
                                                            aGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACg                        >  2:2823577/1‑93 (MQ=255)
                                                             gCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACgg                       >  2:2845213/1‑93 (MQ=255)
                                                             gCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACgg                       <  1:5276430/93‑1 (MQ=255)
                                                               gggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCa                     >  1:6367047/1‑93 (MQ=255)
                                                               gggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCa                     <  1:5829702/93‑1 (MQ=255)
                                                                ggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCag                    <  1:1088166/93‑1 (MQ=255)
                                                                ggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCag                    <  1:3220727/93‑1 (MQ=255)
                                                                ggTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCag                    >  1:4414483/1‑93 (MQ=255)
                                                                 gTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCaga                   >  2:4650527/1‑93 (MQ=255)
                                                                   taataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAgaga                 >  1:1622282/1‑93 (MQ=255)
                                                                   taataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAgaga                 <  2:5277426/93‑1 (MQ=255)
                                                                    aataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGAc                <  1:6444350/93‑1 (MQ=255)
                                                                    aataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGAc                >  1:6312115/1‑93 (MQ=255)
                                                                     ataaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACt               >  1:6331194/1‑93 (MQ=255)
                                                                      taaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTg              >  2:2491843/1‑93 (MQ=255)
                                                                      taaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTg              <  1:901791/93‑1 (MQ=255)
                                                                      taaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTg              >  1:3159115/1‑93 (MQ=255)
                                                                      taaAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTg              <  1:2953051/93‑1 (MQ=255)
                                                                       aaaGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGc             >  2:229119/1‑93 (MQ=255)
                                                                         aGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTCCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCa           <  1:2064354/93‑1 (MQ=255)
                                                                            aaCCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCa        <  2:1800510/93‑1 (MQ=255)
                                                                             aCCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCAt       >  2:2677358/1‑93 (MQ=255)
                                                                             aCCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCAt       >  2:382821/1‑93 (MQ=255)
                                                                              ccAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATa      <  2:3146379/93‑1 (MQ=255)
                                                                                aaGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaa    >  1:664278/1‑93 (MQ=255)
                                                                                aaGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaa    >  2:6082484/1‑93 (MQ=255)
                                                                                 aGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaa   <  2:3334557/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAA  >  NC_000913/4001399‑4001573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAA  >  NC_000913/4001393‑4001572
                                                                                             |                                                                                      
CCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCG                                                                                        <  SRR4302547.6038380/94‑1 (MQ=60)
     CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACGAAGGCTCGCTTCC                                                                                   >  SRR4302547.192620/1‑94 (MQ=60)
     CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCC                                                                                   <  SRR4302547.1149641/94‑1 (MQ=60)
     CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCC                                                                                   <  SRR4302547.3339017/94‑1 (MQ=60)
      AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                                                                                  >  SRR4302547.5600567/1‑94 (MQ=60)
      AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                                                                                  <  SRR4302547.3914585/94‑1 (MQ=60)
      AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                                                                                  <  SRR4302547.5031258/94‑1 (MQ=60)
      AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                                                                                  <  SRR4302547.4735194/94‑1 (MQ=60)
      AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                                                                                  >  SRR4302547.4617638/1‑94 (MQ=60)
       GCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAG                                                                                 >  SRR4302547.3472121/1‑94 (MQ=60)
       GCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAG                                                                                 <  SRR4302547.4384270/94‑1 (MQ=60)
        CCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGC                                                                                <  SRR4302547.1618719/94‑1 (MQ=60)
        CCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGC                                                                                >  SRR4302547.4637122/1‑94 (MQ=60)
         CATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCA                                                                               >  SRR4302547.151853/1‑94 (MQ=60)
         CATTGCATCCGGCGGAATCGATCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGCTAATAAAGTAACCAAGGCTCGCTTCCAGCA                                                                               >  SRR4302547.6551738/1‑94 (MQ=60)
          ATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCAT                                                                              >  SRR4302547.344927/1‑94 (MQ=60)
          ATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCAT                                                                              <  SRR4302547.5645115/94‑1 (MQ=60)
          ATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCAT                                                                              <  SRR4302547.6644118/94‑1 (MQ=60)
           TTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATA                                                                             >  SRR4302547.5727698/1‑94 (MQ=60)
           TTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATA                                                                             >  SRR4302547.5773132/1‑94 (MQ=60)
             GCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATG                                                                           <  SRR4302547.5234424/94‑1 (MQ=60)
             GCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATG                                                                           <  SRR4302547.2010878/94‑1 (MQ=60)
             GCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATG                                                                           <  SRR4302547.6123633/94‑1 (MQ=60)
               ttcaggcgcaatcgcTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAAGCTCGCTACCAGCATATGGT                                                                         <  SRR4302547.1721280/79‑1 (MQ=60)
               ATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGT                                                                         >  SRR4302547.5399688/1‑94 (MQ=60)
               ATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGT                                                                         >  SRR4302547.1754109/1‑94 (MQ=60)
                TCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTG                                                                        <  SRR4302547.4144066/94‑1 (MQ=60)
                TCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTG                                                                        >  SRR4302547.925107/1‑94 (MQ=60)
                 CCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGA                                                                       >  SRR4302547.2617229/1‑94 (MQ=60)
                 CCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGA                                                                       >  SRR4302547.3031715/1‑94 (MQ=60)
                 CCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGA                                                                       >  SRR4302547.3667434/1‑94 (MQ=60)
                  CGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAT                                                                      >  SRR4302547.4615366/1‑94 (MQ=60)
                  CGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAT                                                                      <  SRR4302547.940138/94‑1 (MQ=60)
                     CGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGT                                                                   >  SRR4302547.766255/1‑94 (MQ=60)
                     CGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGT                                                                   >  SRR4302547.983950/1‑94 (MQ=60)
                     CGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGT                                                                   >  SRR4302547.1219286/1‑94 (MQ=60)
                      GGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTT                                                                  <  SRR4302547.4733759/94‑1 (MQ=60)
                      GGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTT                                                                  >  SRR4302547.4896661/1‑94 (MQ=60)
                      GGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTT                                                                  >  SRR4302547.5665343/1‑94 (MQ=60)
                       GAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTC                                                                 <  SRR4302547.3795457/94‑1 (MQ=60)
                       GAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTC                                                                 <  SRR4302547.5280011/94‑1 (MQ=60)
                       GAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTC                                                                 <  SRR4302547.889968/94‑1 (MQ=60)
                           cgcTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCG                                                             >  SRR4302547.3301969/4‑94 (MQ=60)
                             CTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCC                                                           >  SRR4302547.5997151/1‑94 (MQ=60)
                             CTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCC                                                           >  SRR4302547.2035430/1‑94 (MQ=60)
                              TCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCC                                                          >  SRR4302547.1627427/1‑94 (MQ=60)
                              TCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCC                                                          <  SRR4302547.6614365/94‑1 (MQ=60)
                               CGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCA                                                         >  SRR4302547.767065/1‑94 (MQ=60)
                                GCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAG                                                        >  SRR4302547.6572988/1‑94 (MQ=60)
                                GCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAG                                                        <  SRR4302547.6703722/94‑1 (MQ=60)
                                GCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAG                                                        <  SRR4302547.663098/94‑1 (MQ=60)
                                  CGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGAT                                                      >  SRR4302547.3062002/1‑94 (MQ=60)
                                   GAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATA                                                     >  SRR4302547.5870601/1‑94 (MQ=60)
                                    AGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAA                                                    <  SRR4302547.3313271/94‑1 (MQ=60)
                                    AGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAA                                                    >  SRR4302547.197338/1‑94 (MQ=60)
                                    AGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAA                                                    <  SRR4302547.804035/94‑1 (MQ=60)
                                     aaAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAA                                                   <  SRR4302547.2194080/92‑1 (MQ=60)
                                      GAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAAC                                                  >  SRR4302547.5422982/1‑94 (MQ=60)
                                       AAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACA                                                 <  SRR4302547.6086344/94‑1 (MQ=60)
                                        AAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAG                                                <  SRR4302547.5540358/94‑1 (MQ=60)
                                        AAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAG                                                >  SRR4302547.2918637/1‑94 (MQ=60)
                                          ATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTACCCAAGGCACGCTTCCAGCATATGGTGATTTTTCGCCGCCCAGATAAACAGTA                                              <  SRR4302547.3646643/94‑1 (MQ=60)
                                          ATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTA                                              <  SRR4302547.1933814/94‑1 (MQ=60)
                                          ATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTA                                              <  SRR4302547.5988115/94‑1 (MQ=60)
                                          ATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTA                                              <  SRR4302547.1045211/94‑1 (MQ=60)
                                           TCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAG                                             <  SRR4302547.1851512/94‑1 (MQ=60)
                                           TCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAG                                             <  SRR4302547.2561785/94‑1 (MQ=60)
                                           TCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAG                                             <  SRR4302547.3315860/94‑1 (MQ=60)
                                           TCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAG                                             <  SRR4302547.493243/94‑1 (MQ=60)
                                           TCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAG                                             <  SRR4302547.2924098/94‑1 (MQ=60)
                                           TCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAG                                             <  SRR4302547.893539/94‑1 (MQ=60)
                                            CATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGC                                            <  SRR4302547.3497452/94‑1 (MQ=60)
                                              TCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCA                                          >  SRR4302547.831268/1‑94 (MQ=60)
                                                CCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGT                                        >  SRR4302547.5446361/1‑94 (MQ=60)
                                                 CCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTT                                       <  SRR4302547.793320/94‑1 (MQ=60)
                                                  CAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTG                                      >  SRR4302547.160230/1‑94 (MQ=60)
                                                  CAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTG                                      >  SRR4302547.6692150/1‑94 (MQ=60)
                                                  CAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTG                                      <  SRR4302547.659048/94‑1 (MQ=60)
                                                   AGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGC                                     >  SRR4302547.3244363/1‑94 (MQ=60)
                                                    GCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCC                                    <  SRR4302547.2328145/94‑1 (MQ=60)
                                                    GCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCC                                    <  SRR4302547.2753623/94‑1 (MQ=60)
                                                     CACCATGTTCCCCAGCGGGTTTATAATGTAACCAAGGCTCGCTTCCAACATATGGTGATTGTTCACCGCCAAGATAAACAGTAGCCAGGTGCCA                                   <  SRR4302547.2237087/94‑1 (MQ=55)
                                                     CACAATGTTCATCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCA                                   >  SRR4302547.603391/1‑94 (MQ=60)
                                                     CACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCA                                   <  SRR4302547.1742713/94‑1 (MQ=60)
                                                     CACAATGTTCACCAGCGGGATAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCAACGCCCAGATACACAGTAGCCAGTTGCCA                                   >  SRR4302547.2160444/1‑94 (MQ=60)
                                                      ACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCAC                                  >  SRR4302547.3879912/1‑94 (MQ=60)
                                                       CAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACA                                 >  SRR4302547.34044/1‑94 (MQ=60)
                                                        AATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCA                                >  SRR4302547.6063569/1‑94 (MQ=60)
                                                        AATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCA                                >  SRR4302547.6719115/1‑94 (MQ=60)
                                                         ATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAA                               >  SRR4302547.6366823/1‑94 (MQ=60)
                                                         ATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAA                               >  SRR4302547.1095726/1‑94 (MQ=60)
                                                         ATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAA                               <  SRR4302547.2172406/94‑1 (MQ=60)
                                                         ATGTCCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAA                               <  SRR4302547.1384155/94‑1 (MQ=60)
                                                           GTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATC                             <  SRR4302547.4627695/94‑1 (MQ=60)
                                                           GTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATC                             >  SRR4302547.5821437/1‑94 (MQ=60)
                                                            TTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCA                            >  SRR4302547.815769/1‑94 (MQ=60)
                                                            TTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCA                            <  SRR4302547.480634/94‑1 (MQ=60)
                                                              CACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGC                          >  SRR4302547.4024059/1‑94 (MQ=60)
                                                              CACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGC                          >  SRR4302547.281955/1‑94 (MQ=60)
                                                               ACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCA                         >  SRR4302547.4127310/1‑94 (MQ=60)
                                                                CCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAC                        >  SRR4302547.5276506/1‑94 (MQ=60)
                                                                CCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAC                        >  SRR4302547.3002972/1‑94 (MQ=60)
                                                                CCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAC                        >  SRR4302547.1308447/1‑94 (MQ=60)
                                                                CCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCAC                        <  SRR4302547.3764260/94‑1 (MQ=60)
                                                                 CAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACG                       <  SRR4302547.2925374/94‑1 (MQ=60)
                                                                 CAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACG                       >  SRR4302547.3487972/1‑94 (MQ=60)
                                                                 CAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACG                       >  SRR4302547.2101125/1‑94 (MQ=60)
                                                                   GCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGC                     <  SRR4302547.5385222/94‑1 (MQ=60)
                                                                   GCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGC                     <  SRR4302547.5070894/94‑1 (MQ=60)
                                                                    CGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCA                    >  SRR4302547.176551/1‑94 (MQ=60)
                                                                    CGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCA                    >  SRR4302547.6499877/1‑94 (MQ=60)
                                                                     GGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAG                   <  SRR4302547.5950333/94‑1 (MQ=60)
                                                                     GGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAG                   <  SRR4302547.2338742/94‑1 (MQ=60)
                                                                     GGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAG                   >  SRR4302547.4505532/1‑94 (MQ=60)
                                                                      GGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGA                  <  SRR4302547.1109944/94‑1 (MQ=60)
                                                                      GGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGA                  <  SRR4302547.3284967/94‑1 (MQ=60)
                                                                      GGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGA                  >  SRR4302547.530342/1‑94 (MQ=60)
                                                                      GGTTAATAAAGGAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGA                  <  SRR4302547.2775800/94‑1 (MQ=60)
                                                                       GTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAG                 <  SRR4302547.6485173/94‑1 (MQ=60)
                                                                       GTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAG                 <  SRR4302547.4869065/94‑1 (MQ=60)
                                                                        TTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGA                >  SRR4302547.1654382/1‑94 (MQ=60)
                                                                         TAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGAC               <  SRR4302547.6411879/94‑1 (MQ=60)
                                                                         TAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGAC               >  SRR4302547.6443359/1‑94 (MQ=60)
                                                                          AATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACT              >  SRR4302547.6462978/1‑94 (MQ=60)
                                                                          AATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACT              <  SRR4302547.5192654/94‑1 (MQ=60)
                                                                          AATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACT              <  SRR4302547.6578636/94‑1 (MQ=60)
                                                                          AATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACG              >  SRR4302547.5975196/1‑94 (MQ=60)
                                                                           ATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTG             <  SRR4302547.6458803/94‑1 (MQ=60)
                                                                           ATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTG             >  SRR4302547.3221956/1‑94 (MQ=60)
                                                                            TAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGC            <  SRR4302547.3011840/94‑1 (MQ=60)
                                                                            TAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGC            <  SRR4302547.474982/94‑1 (MQ=60)
                                                                            TAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGC            >  SRR4302547.3406979/1‑94 (MQ=60)
                                                                            TAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGC            <  SRR4302547.919152/94‑1 (MQ=60)
                                                                             AAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCC           <  SRR4302547.5346394/94‑1 (MQ=60)
                                                                               AGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAG         <  SRR4302547.2169835/94‑1 (MQ=60)
                                                                               AGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAG         <  SRR4302547.4240783/94‑1 (MQ=60)
                                                                               AGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTCCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAG         <  SRR4302547.2105547/94‑1 (MQ=60)
                                                                                GTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGC        <  SRR4302547.5783999/94‑1 (MQ=60)
                                                                                 TAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCA       <  SRR4302547.6456774/94‑1 (MQ=60)
                                                                                  AACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTATCCAGTTGCCACCAATCAGGACCGCAGAGACTGCCAACAT      >  SRR4302547.2595158/1‑94 (MQ=60)
                                                                                  AACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCAT      <  SRR4302547.3781775/94‑1 (MQ=60)
                                                                                   ACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATA     >  SRR4302547.591736/1‑94 (MQ=60)
                                                                                     CAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAA   >  SRR4302547.676504/1‑94 (MQ=60)
                                                                                     CAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAA   >  SRR4302547.2005975/1‑94 (MQ=60)
                                                                                      AAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAA  >  SRR4302547.5977469/1‑94 (MQ=60)
                                                                                             |                                                                                      
ACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAA  >  NC_000913/4001393‑4001572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑