Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,491 2 bp→CG intergenic (+58/‑174) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4910GC100.0% 700.7 / NA 193intergenic (+58/‑175)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (100/93);  total (100/93)
*NC_0009134,008,4920CG100.0% 678.8 / NA 187intergenic (+59/‑174)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (95/92);  total (95/92)

Only 100 of 196 total aligned reads displayed.

AGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008409‑4008577
                                                                                        ||                                                                                      
gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGGAAG‑GCTGGATTATGGGCAGTGCgcacc                                                                                    >  1:5604860/3‑93 (MQ=255)
gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGGAAG‑GCTGCATTATGGGCAGTGCgcacc                                                                                    >  1:5931997/3‑93 (MQ=255)
gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCgcacc                                                                                    >  2:3643001/3‑93 (MQ=255)
gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCgcacc                                                                                    <  1:3340049/91‑1 (MQ=255)
  cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAg                                                                                  <  1:5836693/93‑1 (MQ=255)
   tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  1:4922669/1‑93 (MQ=255)
   tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  1:866450/1‑93 (MQ=255)
    gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                                                >  2:3864395/1‑93 (MQ=255)
    gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                                                <  2:410613/93‑1 (MQ=255)
      aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGTCAGTGCGCACCAGCGtt                                                                              >  1:1197039/1‑92 (MQ=255)
                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                <  2:5152570/89‑1 (MQ=255)
                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                >  2:6403989/5‑93 (MQ=255)
                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                                                <  1:1056897/89‑1 (MQ=255)
                     ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                                               <  2:929718/90‑1 (MQ=255)
                     ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                                               >  2:175197/4‑93 (MQ=255)
                      tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAGCTTCATcc                                                              >  2:2521224/3‑93 (MQ=255)
                      tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              <  1:5577567/91‑1 (MQ=255)
                        gtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                                            <  1:921788/93‑1 (MQ=255)
                        gtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                                            <  2:5392757/93‑1 (MQ=255)
                         tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa                                                           >  2:5674995/1‑93 (MQ=255)
                         tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa                                                           >  1:2185073/1‑93 (MQ=255)
                         tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATCATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa                                                           >  2:6247931/1‑93 (MQ=255)
                            cGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                        <  1:737491/93‑1 (MQ=255)
                              ggATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                     >  2:2527060/1‑92 (MQ=255)
                               gATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    >  2:5522726/1‑91 (MQ=255)
                               gATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    >  2:1986235/1‑91 (MQ=255)
                                aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                   <  2:5301800/93‑4 (MQ=255)
                                 tGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  >  2:2414163/1‑89 (MQ=255)
                                 tGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  <  1:1068601/93‑5 (MQ=255)
                                    acgacgCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                <  1:1753517/93‑1 (MQ=255)
                                     cgacgCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                               >  1:5113939/1‑93 (MQ=255)
                                     cgacgCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                               >  2:3203999/1‑93 (MQ=255)
                                      gacgCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              <  2:3756621/93‑1 (MQ=255)
                                       acgCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             <  2:6052003/93‑1 (MQ=255)
                                         gCCGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                                           >  1:1206236/1‑93 (MQ=255)
                                           cGAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                         <  1:460624/93‑1 (MQ=255)
                                            gAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  1:2977761/1‑93 (MQ=255)
                                            gAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  2:3489360/1‑93 (MQ=255)
                                            gAAATGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  1:1742587/1‑93 (MQ=255)
                                             aaaTGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                       >  2:3050470/1‑93 (MQ=255)
                                               aTGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     <  2:2542082/93‑1 (MQ=255)
                                               aTGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     <  2:260750/93‑1 (MQ=255)
                                               aTGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     >  2:6487699/1‑93 (MQ=255)
                                               aTGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     <  2:3317426/93‑1 (MQ=255)
                                                tGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  2:4729384/1‑93 (MQ=255)
                                                tGCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  2:6099992/1‑93 (MQ=255)
                                                 gCTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                   <  1:2924265/93‑1 (MQ=255)
                                                  cTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                  <  1:4629335/93‑1 (MQ=255)
                                                  cTGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                  <  1:3534800/93‑1 (MQ=255)
                                                   tGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  2:371689/1‑93 (MQ=255)
                                                   tGTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  1:4101900/1‑93 (MQ=255)
                                                   cgTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 <  1:506992/92‑1 (MQ=255)
                                                    gTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                >  2:1185427/1‑93 (MQ=255)
                                                    gTCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  2:4865780/93‑1 (MQ=255)
                                                     tCGATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               <  2:3028737/93‑1 (MQ=255)
                                                       gATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             >  2:1573723/1‑93 (MQ=255)
                                                       gATGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             <  2:5635097/93‑1 (MQ=255)
                                                        aTGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            >  1:5701826/1‑93 (MQ=255)
                                                        aTGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            <  2:841724/93‑1 (MQ=255)
                                                        aTGGCG‑GGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                            >  1:168750/1‑93 (MQ=255)
                                                           ggcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                          >  1:3672704/5‑93 (MQ=255)
                                                            gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                         >  2:452360/4‑93 (MQ=255)
                                                            gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                         >  1:1820823/4‑93 (MQ=255)
                                                             cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                        <  2:5793374/91‑1 (MQ=255)
                                                             cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                        <  1:4029892/91‑1 (MQ=255)
                                                             cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                        >  1:300252/3‑93 (MQ=255)
                                                              ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       <  1:6312510/92‑1 (MQ=255)
                                                              ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       <  2:3844288/92‑1 (MQ=255)
                                                              ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       <  1:2670173/92‑1 (MQ=255)
                                                               gggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                      >  2:5897441/1‑93 (MQ=255)
                                                                ggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     <  2:2055246/93‑1 (MQ=255)
                                                                  aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTGCTAATGCCGACGACGCGGTGCCg                   <  1:4697058/89‑1 (MQ=255)
                                                                   aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                  >  2:956587/4‑93 (MQ=255)
                                                                   aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                  >  2:3675794/4‑93 (MQ=255)
                                                                   aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                  >  1:5330316/4‑93 (MQ=255)
                                                                   aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                  >  2:1866687/4‑93 (MQ=255)
                                                                    aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                 <  2:3364328/91‑1 (MQ=255)
                                                                    aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                 >  1:6422610/3‑93 (MQ=255)
                                                                     ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                <  1:4290688/92‑1 (MQ=255)
                                                                      gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               <  2:2899699/93‑1 (MQ=255)
                                                                      gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               <  2:388086/93‑1 (MQ=255)
                                                                        tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  2:2126216/93‑1 (MQ=255)
                                                                        tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             <  1:6465690/93‑1 (MQ=255)
                                                                         gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            <  1:1378233/93‑1 (MQ=255)
                                                                          cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  2:1356062/93‑1 (MQ=255)
                                                                          cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  1:6020153/93‑1 (MQ=255)
                                                                          cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  1:3570102/93‑1 (MQ=255)
                                                                           aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg          >  1:980118/1‑93 (MQ=255)
                                                                           aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg          <  1:5221782/93‑1 (MQ=255)
                                                                           aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg          <  1:4382926/93‑1 (MQ=255)
                                                                           aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg          >  2:2161658/1‑93 (MQ=255)
                                                                              aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt       <  1:4776536/93‑1 (MQ=255)
                                                                              aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt       <  1:2263131/93‑1 (MQ=255)
                                                                               tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa      <  2:3982457/93‑1 (MQ=255)
                                                                                gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa     >  2:1373889/1‑93 (MQ=255)
                                                                                 ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa    >  2:5131349/1‑93 (MQ=255)
                                                                                 ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa    >  1:6153694/1‑93 (MQ=255)
                                                                                  gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc   <  1:5462994/93‑1 (MQ=255)
                                                                                  gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc   >  2:660382/1‑93 (MQ=255)
                                                                                   cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct  <  1:3048197/93‑1 (MQ=255)
                                                                                        ||                                                                                      
AGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008409‑4008577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008404‑4008579
                                                                                                |                                                                                         
GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGC                                                                                           <  SRR4302547.2571947/94‑1 (MQ=60)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGG‑‑AAG‑GCTGGATTATGGGCAGTGCGCACC                                                                                      >  SRR4302547.5720488/4‑94 (MQ=50)
     agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGG‑‑AAG‑GCTGCATTATGGGCAGTGCGCACC                                                                                      >  SRR4302547.6054993/4‑94 (MQ=60)
      gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCA                                                                                     <  SRR4302547.3407643/92‑1 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    <  SRR4302547.6362301/94‑1 (MQ=60)
      C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAG                                                                                    <  SRR4302547.5392178/94‑1 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302547.883027/1‑94 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   <  SRR4302547.4123631/94‑1 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   <  SRR4302547.5957459/94‑1 (MQ=60)
        CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGC                                                                                   >  SRR4302547.5024625/1‑94 (MQ=60)
         TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCG                                                                                  >  SRR4302547.3313888/1‑94 (MQ=57)
         TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCG                                                                                  >  SRR4302547.5004293/1‑94 (MQ=57)
         TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCG                                                                                  <  SRR4302547.5183144/94‑1 (MQ=57)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGTCAGTGCGCACCAGCGTT                                                                                >  SRR4302547.1221192/1‑94 (MQ=44)
           AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTC                                                                                >  SRR4302547.1874931/1‑94 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                               <  SRR4302547.6046996/94‑1 (MQ=57)
            AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCT                                                                               <  SRR4302547.4432855/94‑1 (MQ=57)
             GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                                              >  SRR4302547.2076823/1‑94 (MQ=60)
                 tgtattgcgtttgGTGACGGTATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          <  SRR4302547.4865767/81‑1 (MQ=60)
                 tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                                          >  SRR4302547.1805044/14‑94 (MQ=60)
                  gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                                         >  SRR4302547.3204810/13‑94 (MQ=60)
                  T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGCAAG‑GCTGCATTATGGGCAGTGCGCACCAGAGTCTGAAAGAC                                                                        >  SRR4302547.4030477/1‑94 (MQ=60)
                  T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                                        >  SRR4302547.3631094/1‑94 (MQ=60)
                    ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                                       >  SRR4302547.5565756/1‑94 (MQ=60)
                      TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTT                                                                     >  SRR4302547.4729456/1‑94 (MQ=60)
                       GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC                                                                    >  SRR4302547.1853310/1‑94 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302547.1029109/1‑94 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302547.4292256/1‑94 (MQ=60)
                        CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302547.6644422/1‑94 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302547.3100901/89‑1 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302547.6373042/89‑1 (MQ=60)
                         gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302547.1779384/89‑1 (MQ=60)
                          tttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATC                                                                 <  SRR4302547.1077919/90‑1 (MQ=60)
                          TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                                               <  SRR4302547.5692588/94‑1 (MQ=60)
                              GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                                             >  SRR4302547.2228876/1‑94 (MQ=60)
                              GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                                             <  SRR4302547.939594/94‑1 (MQ=60)
                                GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCACCCCGAGC                                                           >  SRR4302547.811310/1‑94 (MQ=60)
                                  CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        <  SRR4302547.751299/94‑1 (MQ=60)
                                  CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        >  SRR4302547.5830561/1‑94 (MQ=60)
                                   GGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                       >  SRR4302547.5615165/1‑94 (MQ=60)
                                   GGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                       >  SRR4302547.3976144/1‑94 (MQ=60)
                                      ATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     <  SRR4302547.308281/94‑1 (MQ=60)
                                       TGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                    <  SRR4302547.1089880/94‑1 (MQ=60)
                                         AACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                  >  SRR4302547.1198134/1‑94 (MQ=60)
                                          ACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                 <  SRR4302547.1788185/94‑1 (MQ=60)
                                          ACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                 >  SRR4302547.5219323/1‑94 (MQ=60)
                                           CGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                <  SRR4302547.3240957/94‑1 (MQ=60)
                                            tactgcggcataattcattagattggggggaaaggcttaATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                  <  SRR4302547.2189780/55‑1 (MQ=34)
                                            GACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302547.888404/94‑1 (MQ=60)
                                            GACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302547.2289714/94‑1 (MQ=60)
                                              CGCCGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             >  SRR4302547.1230577/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          <  SRR4302547.469134/94‑1 (MQ=60)
                                                 CGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302547.3037007/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302547.1776886/1‑94 (MQ=60)
                                                 CGAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302547.1224111/1‑94 (MQ=60)
                                                  GAAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         >  SRR4302547.5253826/1‑94 (MQ=60)
                                                   AAATGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                        >  SRR4302547.544902/1‑94 (MQ=60)
                                                      TGCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                     <  SRR4302547.6312935/94‑1 (MQ=60)
                                                       GCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    >  SRR4302547.1778203/1‑94 (MQ=60)
                                                       GCTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    <  SRR4302547.2982575/94‑1 (MQ=60)
                                                        CTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   <  SRR4302547.3606970/94‑1 (MQ=60)
                                                        CTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302547.3699325/1‑94 (MQ=60)
                                                        CTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302547.6534897/1‑94 (MQ=60)
                                                        CTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302547.4185309/1‑94 (MQ=60)
                                                        CTGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   <  SRR4302547.4725225/94‑1 (MQ=60)
                                                         CGTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                  <  SRR4302547.516194/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302547.5780534/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302547.3492111/94‑1 (MQ=60)
                                                          GTCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302547.4348088/94‑1 (MQ=60)
                                                           TCGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                >  SRR4302547.532665/1‑94 (MQ=60)
                                                            CGATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                               <  SRR4302547.3621421/94‑1 (MQ=60)
                                                             GATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              >  SRR4302547.5819606/1‑94 (MQ=60)
                                                             GATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              >  SRR4302547.508670/1‑94 (MQ=60)
                                                             GATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302547.6580736/94‑1 (MQ=60)
                                                             GATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              >  SRR4302547.171841/1‑94 (MQ=60)
                                                              ATGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                             >  SRR4302547.5767409/1‑94 (MQ=60)
                                                               TGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                            >  SRR4302547.3747605/1‑94 (MQ=60)
                                                               TGGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                            >  SRR4302547.4852321/1‑94 (MQ=60)
                                                                GGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           >  SRR4302547.1856817/1‑94 (MQ=60)
                                                                GGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           >  SRR4302547.568183/1‑94 (MQ=60)
                                                                GGC‑‑GGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302547.4499608/94‑1 (MQ=60)
                                                                    gcggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGG                          >  SRR4302547.2430988/11‑94 (MQ=60)
                                                                    gcggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGG                          >  SRR4302547.305855/11‑94 (MQ=60)
                                                                     cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                         <  SRR4302547.4111755/85‑1 (MQ=60)
                                                                     cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                         >  SRR4302547.1739451/10‑94 (MQ=60)
                                                                     GGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                       >  SRR4302547.2985126/1‑94 (MQ=60)
                                                                     GGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                       >  SRR4302547.4703879/1‑94 (MQ=60)
                                                                      ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302547.6443764/86‑1 (MQ=60)
                                                                      ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302547.2723814/86‑1 (MQ=60)
                                                                          AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                   >  SRR4302547.6556496/1‑94 (MQ=60)
                                                                          AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                   >  SRR4302547.2785673/1‑94 (MQ=60)
                                                                          AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTGCTAATGCCGACGACGCGGTGCCGC                    <  SRR4302547.4794351/94‑1 (MQ=60)
                                                                          AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                    <  SRR4302547.6712459/94‑1 (MQ=60)
                                                                          AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                    >  SRR4302547.5440187/1‑94 (MQ=60)
                                                                            G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                 <  SRR4302547.4378874/94‑1 (MQ=60)
                                                                              GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                <  SRR4302547.3055973/94‑1 (MQ=60)
                                                                                TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              <  SRR4302547.6600330/94‑1 (MQ=60)
                                                                                 GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             <  SRR4302547.1405862/94‑1 (MQ=60)
                                                                                 GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             >  SRR4302547.1533375/1‑94 (MQ=60)
                                                                                  CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            >  SRR4302547.999191/1‑94 (MQ=60)
                                                                                  CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302547.3642936/94‑1 (MQ=60)
                                                                                  CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302547.6145100/94‑1 (MQ=60)
                                                                                  CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302547.611167/94‑1 (MQ=60)
                                                                                   ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           <  SRR4302547.4473080/94‑1 (MQ=60)
                                                                                   ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           <  SRR4302547.5329233/94‑1 (MQ=60)
                                                                                    TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302547.1382198/94‑1 (MQ=60)
                                                                                     TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         >  SRR4302547.614677/1‑94 (MQ=60)
                                                                                      ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA        <  SRR4302547.4875493/94‑1 (MQ=60)
                                                                                      ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA        <  SRR4302547.2308866/94‑1 (MQ=60)
                                                                                        GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA      >  SRR4302547.1797251/1‑94 (MQ=60)
                                                                                        GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA      >  SRR4302547.6281375/1‑94 (MQ=60)
                                                                                         GGCAGTGCGCACGAGCGGCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC     <  SRR4302547.999065/94‑1 (MQ=60)
                                                                                          GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT    <  SRR4302547.5575843/94‑1 (MQ=60)
                                                                                           CAGTGCGCACCAGCGTCTGAAAGGCCTTCCTCCCGGGATGGCAGTGTTTGGTTCTCATGCCGACGTCGCGGTGCCGCATTATCTGCGTAAACTC   <  SRR4302547.6786390/94‑1 (MQ=23)
                                                                                           CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   <  SRR4302547.3108841/94‑1 (MQ=60)
                                                                                            AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  <  SRR4302547.5520832/94‑1 (MQ=60)
                                                                                            AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  <  SRR4302547.5110872/94‑1 (MQ=60)
                                                                                            AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  SRR4302547.3675527/1‑94 (MQ=60)
                                                                                                |                                                                                         
GCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008404‑4008579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑