Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,055,398	A→G	S73P (TCC→CCC)	glnA ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,055,398	0	A	G	99.6%	1003.2 / ‑4.6	278	S73P (TCC→CCC)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (130/147); total (131/147)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-01

Only 100 of 299 total aligned reads displayed.

GGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGATGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGA  >  NC_000913/4055308‑4055487

ggTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCggtgggtg                                                                                         >  2:3503383/1‑93 (MQ=255)

ggTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCggtgggtg                                                                                         >  2:5933290/1‑93 (MQ=255)

ggTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCggtgggtg                                                                                         <  2:6228805/93‑1 (MQ=255)

  tCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCg                                                                                       <  2:1749487/93‑1 (MQ=255)

    aTAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTTGGTGCGTc                                                                                     >  1:6174155/1‑93 (MQ=255)

    aTAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTc                                                                                     <  1:1190585/93‑1 (MQ=255)

     tAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCt                                                                                    >  2:2163970/1‑93 (MQ=255)

     tAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCt                                                                                    >  2:3732640/1‑93 (MQ=255)

      aGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTg                                                                                   <  2:684941/93‑1 (MQ=255)

      aGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTg                                                                                   <  1:4620496/93‑1 (MQ=255)

         cTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCa                                                                                >  1:5515422/1‑93 (MQ=255)

          ttGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCAt                                                                               >  2:4535177/1‑93 (MQ=255)

          ttGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCAt                                                                               >  2:1314965/1‑93 (MQ=255)

           tGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATc                                                                              >  2:3258413/1‑93 (MQ=255)

           tGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATc                                                                              <  2:4695853/93‑1 (MQ=255)

            gCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCa                                                                             <  1:5889578/93‑1 (MQ=255)

            gCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCa                                                                             >  2:4218665/1‑93 (MQ=255)

             cAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAg                                                                            <  1:2325723/93‑1 (MQ=255)

             cAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAg                                                                            <  2:6092736/93‑1 (MQ=255)

             cAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAg                                                                            <  1:580926/93‑1 (MQ=255)

              aGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGc                                                                           >  2:6534323/1‑93 (MQ=255)

                     cAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGt                                                                    >  2:5579920/1‑93 (MQ=255)

                     cAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGt                                                                    <  1:6043673/93‑1 (MQ=255)

                        gTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCTTCAGCACCATGTCgg                                                                 >  1:910740/1‑93 (MQ=255)

                        gTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCgg                                                                 >  1:1311315/1‑93 (MQ=255)

                         ttCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGa                                                                >  2:6486197/1‑93 (MQ=255)

                          tCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGAc                                                               <  1:3489954/93‑1 (MQ=255)

                          tCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGAc                                                               >  2:3986032/1‑93 (MQ=255)

                           cAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTGTGGCATCAGCACCATGTCGGAcc                                                              >  2:4845474/1‑92 (MQ=255)

                           cAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACt                                                              <  2:4041576/93‑1 (MQ=255)

                            aaGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTc                                                             <  2:5447340/93‑1 (MQ=255)

                            aaGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTc                                                             >  1:2434193/1‑93 (MQ=255)

                             aGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCg                                                            >  2:3136650/1‑93 (MQ=255)

                              ggATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGt                                                           <  1:1393661/93‑1 (MQ=255)

                                  gTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAAt                                                       >  2:3310814/1‑93 (MQ=255)

                                    cGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGc                                                     <  2:3413584/93‑1 (MQ=255)

                                    cGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGc                                                     <  2:909412/93‑1 (MQ=255)

                                     gCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGcc                                                    <  2:2055536/93‑1 (MQ=255)

                                     gCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGcc                                                    >  2:3028846/1‑93 (MQ=255)

                                         cGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTc                                                <  2:431176/93‑1 (MQ=255)

                                          ggATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTcc                                               >  1:5600405/1‑93 (MQ=255)

                                          ggATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTcc                                               <  1:4482869/93‑1 (MQ=255)

                                          ggATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTcc                                               <  2:1130985/93‑1 (MQ=255)

                                           gATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCa                                              <  2:3604007/93‑1 (MQ=255)

                                              aaTCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAgcc                                           <  2:4384443/93‑1 (MQ=255)

                                               aTCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAgccg                                          <  2:1733021/93‑1 (MQ=255)

                                                tCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAgccgc                                         <  1:1242410/93‑1 (MQ=255)

                                                tCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAgccgc                                         >  1:6519816/1‑93 (MQ=255)

                                                tCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAgccgc                                         >  2:2593868/1‑93 (MQ=255)

                                                 cAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAgccgcc                                        <  2:2310028/93‑1 (MQ=255)

                                                 cAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAgccgcc                                        <  1:3332484/93‑1 (MQ=255)

                                                  aGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCa                                       <  2:1264481/93‑1 (MQ=255)

                                                  aGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCa                                       >  2:5628145/1‑93 (MQ=255)

                                                    ggTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAAt                                     <  1:3951004/93‑1 (MQ=255)

                                                    ggTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAAt                                     >  1:6582575/1‑93 (MQ=255)

                                                     gTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATc                                    >  1:2236551/1‑93 (MQ=255)

                                                      tGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATcc                                   <  1:6380934/93‑2 (MQ=255)

                                                      tGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCg                                   <  1:4260714/93‑1 (MQ=255)

                                                       ggAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCgc                                  >  2:1645819/1‑92 (MQ=255)

                                                        gAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCgag                                 <  1:1943401/93‑1 (MQ=255)

                                                        gAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCgag                                 <  2:1056330/93‑1 (MQ=255)

                                                         aGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCgagg                                <  1:1700432/93‑1 (MQ=255)

                                                          gTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCgagga                               <  1:4355788/93‑1 (MQ=255)

                                                             ggCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGcc                            <  2:548603/93‑1 (MQ=255)

                                                             ggCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGcc                            <  2:3884016/93‑1 (MQ=255)

                                                                gaagaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTc                         >  1:5394972/1‑93 (MQ=255)

                                                                gaagaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTc                         <  2:1486055/93‑1 (MQ=255)

                                                                gaagaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTc                         <  2:6355666/93‑1 (MQ=255)

                                                                 aagaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCa                        >  1:5854712/1‑93 (MQ=255)

                                                                 aagaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCa                        >  2:4080458/1‑93 (MQ=255)

                                                                  agaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCaa                       >  1:4937436/1‑93 (MQ=255)

                                                                   gaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCaac                      >  2:2486859/1‑92 (MQ=255)

                                                                   gaaCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCaaa                      >  1:4138269/1‑93 (MQ=255)

                                                                     aCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACa                    >  1:6159438/1‑93 (MQ=255)

                                                                     aCGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACa                    >  1:1765585/1‑93 (MQ=255)

                                                                      cGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACAt                   <  1:3723619/93‑1 (MQ=255)

                                                                       gggTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACAtt                  >  2:5721057/1‑93 (MQ=255)

                                                                         gTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACAtttt                <  2:1925635/93‑1 (MQ=255)

                                                                         gTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACAtttt                <  1:4764703/93‑1 (MQ=255)

                                                                          tCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTg               >  2:5380389/1‑93 (MQ=255)

                                                                            aaTCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGcc             <  2:3791976/93‑1 (MQ=255)

                                                                            aaTCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGcc             <  2:2738463/93‑1 (MQ=255)

                                                                            aaTCACTGAGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCTAGTAGCCGTCTAACATTTTgtc             >  1:6465667/1‑91 (MQ=255)

                                                                             aTCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCct            >  1:3198572/1‑93 (MQ=255)

                                                                             aTCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCct            >  1:4197848/1‑93 (MQ=255)

                                                                             aTCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCct            <  1:1515390/93‑1 (MQ=255)

                                                                              tCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCctt           <  2:1395889/93‑1 (MQ=255)

                                                                                aCTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCcttct         >  1:4228642/1‑93 (MQ=255)

                                                                                aCTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCcttct         <  2:2131151/93‑1 (MQ=255)

                                                                                aCTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCcttct         <  2:5827358/93‑1 (MQ=255)

                                                                                 cTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCcttctt        <  2:5173670/93‑1 (MQ=255)

                                                                                  tGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCttcttc       <  1:273879/93‑1 (MQ=255)

                                                                                  tGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCttcttc       <  1:1064651/93‑1 (MQ=255)

                                                                                   gCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCg      <  2:2371144/93‑1 (MQ=255)

                                                                                     ggtgggtgCGTCTGGCATCAGCACCATGTCGGCCTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCgaa    <  1:1007956/93‑1 (MQ=255)

                                                                                     ggtgggtgCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCgaa    <  2:5317173/93‑1 (MQ=255)

                                                                                     ggtgggtgCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCgaa    <  2:2948328/93‑1 (MQ=255)

                                                                                      gTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCgaag   <  1:785124/93‑1 (MQ=255)

                                                                                       tGGGTGCGTCTGGCATCAGCGCCATGTAGGTCTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCgaaga  <  2:4352925/93‑1 (MQ=255)

                                                                                       tGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCgaaga  <  1:6334937/93‑1 (MQ=255)

GGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGATGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGA  >  NC_000913/4055308‑4055487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACGGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGATGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGAATTC  >  NC_000913/4055305‑4055491

CACGGTCATATCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGG                                                                                               >  SRR4302547.4787520/1‑94 (MQ=60)

CACGGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGG                                                                                               >  SRR4302547.5750752/1‑94 (MQ=60)

 ACGGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGT                                                                                              <  SRR4302547.6361630/94‑1 (MQ=60)

 ACGGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGT                                                                                              <  SRR4302547.6729392/94‑1 (MQ=60)

 ACGGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGAAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGT                                                                                              <  SRR4302547.907145/94‑1 (MQ=60)

  CGGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTG                                                                                             <  SRR4302547.4752032/94‑1 (MQ=60)

   GGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGC                                                                                            >  SRR4302547.5976040/1‑94 (MQ=60)

    GTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCG                                                                                           >  SRR4302547.1004358/1‑94 (MQ=60)

      CATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTTGGTGCGTC                                                                                         >  SRR4302547.6302315/1‑94 (MQ=60)

      CATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTC                                                                                         >  SRR4302547.4334466/1‑94 (MQ=60)

       ATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCT                                                                                        <  SRR4302547.1214613/94‑1 (MQ=60)

         AGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGG                                                                                      <  SRR4302547.4716177/94‑1 (MQ=60)

         AGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGG                                                                                      <  SRR4302547.5074862/94‑1 (MQ=60)

         AGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGG                                                                                      <  SRR4302547.6605287/94‑1 (MQ=60)

           CCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCA                                                                                    >  SRR4302547.5629282/1‑94 (MQ=60)

            CTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCAT                                                                                   >  SRR4302547.4245685/1‑94 (MQ=60)

              TGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCA                                                                                 >  SRR4302547.1045956/1‑94 (MQ=60)

               GCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAG                                                                                <  SRR4302547.2289149/94‑1 (MQ=60)

               GCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAG                                                                                <  SRR4302547.6011511/94‑1 (MQ=60)

                CAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGC                                                                               <  SRR4302547.2372856/94‑1 (MQ=60)

                CAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGC                                                                               <  SRR4302547.591351/94‑1 (MQ=60)

                CAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGC                                                                               <  SRR4302547.6764141/94‑1 (MQ=60)

                 AGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGGATCAGCA                                                                              >  SRR4302547.4891218/1‑94 (MQ=60)

                 AGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCA                                                                              >  SRR4302547.6349696/1‑94 (MQ=60)

                   GGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACC                                                                            >  SRR4302547.3619619/1‑94 (MQ=60)

                     TGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCAT                                                                          <  SRR4302547.4801166/94‑1 (MQ=60)

                       CCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGT                                                                        >  SRR4302547.311900/1‑94 (MQ=60)

                        CAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTC                                                                       >  SRR4302547.968553/1‑94 (MQ=60)

                        CAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTC                                                                       <  SRR4302547.4685132/94‑1 (MQ=60)

                        CAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTC                                                                       <  SRR4302547.6169067/94‑1 (MQ=60)

                        CAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTC                                                                       >  SRR4302547.2377610/1‑94 (MQ=60)

                         AGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCG                                                                      <  SRR4302547.2997487/94‑1 (MQ=60)

                         AGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCG                                                                      <  SRR4302547.1398076/94‑1 (MQ=60)

                          GGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCTTCAGCACCATGTCGG                                                                     >  SRR4302547.928292/1‑94 (MQ=60)

                          GGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGG                                                                     >  SRR4302547.1337686/1‑94 (MQ=60)

                           GTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGA                                                                    >  SRR4302547.4181942/1‑94 (MQ=60)

                           GTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGA                                                                    >  SRR4302547.3317516/1‑94 (MQ=60)

                             TCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACT                                                                  <  SRR4302547.3561170/94‑1 (MQ=60)

                              CAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTC                                                                 >  SRR4302547.2483438/1‑94 (MQ=60)

                                 GGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTT                                                              <  SRR4302547.1421545/94‑1 (MQ=60)

                                 GGATGTCGCAACGGATAATCACGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGGCGGACTCGTT                                                              >  SRR4302547.5535082/1‑94 (MQ=60)

                                  GATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTA                                                             >  SRR4302547.6147591/1‑94 (MQ=60)

                                    TGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAAT                                                           <  SRR4302547.4477131/94‑1 (MQ=60)

                                    TGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAAT                                                           <  SRR4302547.5964969/94‑1 (MQ=60)

                                     GTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATG                                                          <  SRR4302547.6707481/94‑1 (MQ=60)

                                        GCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCT                                                       <  SRR4302547.133125/94‑1 (MQ=60)

                                        GCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCT                                                       >  SRR4302547.1548710/1‑94 (MQ=60)

                                          AACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTT                                                     <  SRR4302547.6281443/94‑1 (MQ=60)

                                            CGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCC                                                   >  SRR4302547.5715932/1‑94 (MQ=60)

                                             GGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCA                                                  <  SRR4302547.6261985/94‑1 (MQ=60)

                                             GGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCA                                                  <  SRR4302547.4575482/94‑1 (MQ=60)

                                               ATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGC                                                >  SRR4302547.4023909/1‑94 (MQ=60)

                                               ATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGC                                                >  SRR4302547.2396632/1‑94 (MQ=60)

                                               ATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGC                                                >  SRR4302547.1441906/1‑94 (MQ=60)

                                                 AATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCG                                              <  SRR4302547.4862379/94‑1 (MQ=60)

                                                 AATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCG                                              <  SRR4302547.2228144/94‑1 (MQ=60)

                                                 AATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCG                                              <  SRR4302547.3822528/94‑1 (MQ=60)

                                                 AATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCG                                              >  SRR4302547.6302204/1‑94 (MQ=60)

                                                 AATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCG                                              >  SRR4302547.1148767/1‑94 (MQ=60)

                                                  ATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGC                                             >  SRR4302547.6655556/1‑94 (MQ=60)

                                                  ATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGC                                             <  SRR4302547.4476508/94‑1 (MQ=60)

                                                   TCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCC                                            <  SRR4302547.1267487/94‑1 (MQ=60)

                                                    CAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCA                                           >  SRR4302547.1643925/1‑94 (MQ=60)

                                                    CAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCA                                           >  SRR4302547.6534774/1‑94 (MQ=60)

                                                    CAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCA                                           <  SRR4302547.3399879/94‑1 (MQ=60)

                                                    CAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCA                                           >  SRR4302547.6008425/1‑94 (MQ=60)

                                                    CAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCA                                           <  SRR4302547.4372378/94‑1 (MQ=60)

                                                     AGGGTGGAGTCGGCGAAGAACGGGGCAATCACTGCGGTGGGTGCGTCTGGGATCAGCACCATGTCGGACTCGTTAATGACTTTCCAGCCGCCAA                                          >  SRR4302547.4259722/1‑94 (MQ=60)

                                                      GGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAAT                                         >  SRR4302547.6719649/1‑94 (MQ=60)

                                                       GGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATC                                        <  SRR4302547.4031363/94‑1 (MQ=60)

                                                       GGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATC                                        >  SRR4302547.2584518/1‑94 (MQ=60)

                                                       GGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATC                                        >  SRR4302547.2281652/1‑94 (MQ=60)

                                                       GGGGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATC                                        <  SRR4302547.1269603/94‑1 (MQ=60)

                                                         TGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGG                                      >  SRR4302547.4581160/1‑94 (MQ=60)

                                                         TGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGA                                      <  SRR4302547.4347904/94‑1 (MQ=60)

                                                         TGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGA                                      >  SRR4302547.5250090/1‑94 (MQ=60)

                                                         TGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGA                                      <  SRR4302547.1223485/94‑1 (MQ=60)

                                                         TGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCCA                                      <  SRR4302547.6514091/94‑1 (MQ=60)

                                                          GGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAG                                     >  SRR4302547.709208/1‑94 (MQ=60)

                                                          GGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAG                                     <  SRR4302547.3451204/94‑1 (MQ=60)

                                                          GGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAG                                     >  SRR4302547.6513420/1‑94 (MQ=60)

                                                           GAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCCATCGAGG                                    >  SRR4302547.1384186/1‑94 (MQ=60)

                                                           GAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGG                                    <  SRR4302547.1981936/94‑1 (MQ=60)

                                                            AGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGA                                   >  SRR4302547.6298736/1‑94 (MQ=60)

                                                            AGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGA                                   <  SRR4302547.6655287/94‑1 (MQ=60)

                                                            AGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGA                                   <  SRR4302547.1733848/94‑1 (MQ=60)

                                                            AGTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGA                                   <  SRR4302547.1219981/94‑1 (MQ=60)

                                                             GTCGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAG                                  <  SRR4302547.4445261/94‑1 (MQ=60)

                                                               CGGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCC                                <  SRR4302547.87063/94‑1 (MQ=60)

                                                                GGCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCG                               >  SRR4302547.4298784/1‑94 (MQ=60)

                                                                 GCGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCTTCAGCACCATGTCGGACTCGTTAATGCCTTCCCAGCCGCCAATCGAGGAGCCGT                              <  SRR4302547.6125583/94‑1 (MQ=60)

                                                                  CGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTC                             >  SRR4302547.5506423/1‑94 (MQ=60)

                                                                  CGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTC                             >  SRR4302547.2632802/1‑94 (MQ=60)

                                                                  CGAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTC                             <  SRR4302547.906761/94‑1 (MQ=60)

                                                                   GAAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCA                            >  SRR4302547.5975852/1‑94 (MQ=60)

                                                                    AAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAA                           >  SRR4302547.5039702/1‑94 (MQ=60)

                                                                    AAGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAA                           <  SRR4302547.5687352/94‑1 (MQ=60)

                                                                     AGAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAA                          >  SRR4302547.4222469/1‑94 (MQ=60)

                                                                      GAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAAC                         <  SRR4302547.4985505/94‑1 (MQ=60)

                                                                      GAACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAAC                         >  SRR4302547.1172802/1‑94 (MQ=60)

                                                                       AACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACA                        >  SRR4302547.6287271/1‑94 (MQ=60)

                                                                       AACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACA                        <  SRR4302547.5938227/94‑1 (MQ=60)

                                                                       AACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACA                        <  SRR4302547.1922508/94‑1 (MQ=60)

                                                                       AACGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACA                        >  SRR4302547.1800606/1‑94 (MQ=60)

                                                                         CGGGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATT                      <  SRR4302547.3799630/94‑1 (MQ=60)

                                                                           GGTCAATCCCGGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTT                    <  SRR4302547.1746463/94‑1 (MQ=60)

                                                                           GGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTT                    <  SRR4302547.655104/94‑1 (MQ=60)

                                                                           GGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTT                    <  SRR4302547.2103362/94‑1 (MQ=60)

                                                                           GGTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTT                    <  SRR4302547.5719843/94‑1 (MQ=60)

                                                                            GTCAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTG                   <  SRR4302547.4863404/94‑1 (MQ=60)

                                                                              CAATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGACTTTCCAGCCGACAATCGAGGAGCCGTCAAACATTTTGAC                 >  SRR4302547.823619/1‑94 (MQ=60)

                                                                              CAATCACTGAGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCTAGTAGCCGTCTAACATTTTGTC                 >  SRR4302547.6600306/1‑94 (MQ=60)

                                                                               AATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCT                >  SRR4302547.4283453/1‑94 (MQ=60)

                                                                               AATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCT                >  SRR4302547.3262335/1‑94 (MQ=60)

                                                                               AATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCT                >  SRR4302547.3385781/1‑94 (MQ=60)

                                                                               AATCACTGCGGTGGATGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCT                >  SRR4302547.5668238/1‑94 (MQ=60)

                                                                                ATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTT               >  SRR4302547.4120522/1‑94 (MQ=60)

                                                                                ATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTT               <  SRR4302547.5587060/94‑1 (MQ=60)

                                                                                ATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTT               <  SRR4302547.6512922/94‑1 (MQ=60)

                                                                                ATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTT               <  SRR4302547.1545585/94‑1 (MQ=60)

                                                                                ATCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTT               <  SRR4302547.232633/94‑1 (MQ=60)

                                                                                 TCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTC              <  SRR4302547.3417799/94‑1 (MQ=60)

                                                                                 TCACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTC              <  SRR4302547.1847797/94‑1 (MQ=60)

                                                                                  CACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCT             >  SRR4302547.4315071/1‑94 (MQ=60)

                                                                                   CCTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTT            <  SRR4302547.1747114/94‑1 (MQ=60)

                                                                                   ACTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTT            <  SRR4302547.6431569/94‑1 (MQ=60)

                                                                                    CTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTCCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTC           <  SRR4302547.6702347/94‑1 (MQ=60)

                                                                                    CTGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATCCCTTTCCAGCCCCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTC           <  SRR4302547.5371585/94‑1 (MQ=60)

                                                                                     TGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCG          <  SRR4302547.278998/94‑1 (MQ=60)

                                                                                     TGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCG          <  SRR4302547.1085841/94‑1 (MQ=60)

                                                                                     TGCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCG          <  SRR4302547.437880/94‑1 (MQ=60)

                                                                                      GCGGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGA         >  SRR4302547.145824/1‑94 (MQ=60)

                                                                                        GGTGGGTGCGTCTGGCATCAGCACCATGTCGGCCTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAG       <  SRR4302547.1027636/94‑1 (MQ=60)

                                                                                        GGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAG       >  SRR4302547.5886950/1‑94 (MQ=60)

                                                                                        GGTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAG       >  SRR4302547.4300018/1‑94 (MQ=60)

                                                                                         GTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGA      >  SRR4302547.3585589/1‑94 (MQ=60)

                                                                                         GTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGA      <  SRR4302547.799976/94‑1 (MQ=60)

                                                                                         GTGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGA      <  SRR4302547.4346512/94‑1 (MQ=60)

                                                                                          TGGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGAA     <  SRR4302547.6466839/94‑1 (MQ=60)

                                                                                           GGGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGAAT    <  SRR4302547.5491044/94‑1 (MQ=60)

                                                                                            GGTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGAATT   >  SRR4302547.6388662/1‑94 (MQ=60)

                                                                                             GTGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGAATTC  <  SRR4302547.2373175/94‑1 (MQ=60)

CACGGTCATAGCCTTGCAGGGTGCCAGGTTCAAGGATGTCGCAACGGATAATCAGGGTGGAGTCGGCGAAGAACGGGTCAATCACTGCGGTGGATGCGTCTGGCATCAGCACCATGTCGGACTCGTTAATGCCTTTCCAGCCGCCAATCGAGGAGCCGTCAAACATTTTGCCTTCTTCGAAGAATTC  >  NC_000913/4055305‑4055491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`