Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,172,320 T→C S96G (AGT→GGT)  coaA ← pantothenate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,172,3200TC89.6% 723.5 / 63.4 252S96G (AGT→GGT) coaApantothenate kinase
Reads supporting (aligned to +/- strand):  ref base T (11/15);  new base C (129/96);  total (141/111)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.07e-01

Only 100 of 261 total aligned reads displayed.

TGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATA  >  NC_000913/4172228‑4172411
                                                                                            |                                                                                           
tGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACt                                                                                             <  1:988845/93‑1 (MQ=255)
   aGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGcc                                                                                          >  2:6001392/1‑93 (MQ=255)
   aGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGcc                                                                                          <  1:548820/93‑1 (MQ=255)
     tGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAg                                                                                        <  1:1044936/93‑1 (MQ=255)
      gATCAGTTCAAAACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGc                                                                                       >  1:1747831/1‑93 (MQ=255)
       aTCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCa                                                                                      >  2:3729019/1‑93 (MQ=255)
        tCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCaa                                                                                     >  1:4185197/1‑93 (MQ=255)
        tCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCaa                                                                                     <  2:2886576/93‑1 (MQ=255)
        tCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCaa                                                                                     <  2:318302/93‑1 (MQ=255)
           gTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATAc                                                                                  >  1:2121809/1‑93 (MQ=255)
            ttCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACt                                                                                 >  2:2110845/1‑93 (MQ=255)
             tCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTg                                                                                <  2:6277614/93‑1 (MQ=255)
               aaCACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGAt                                                                              >  2:3297380/1‑93 (MQ=255)
                acacGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATa                                                                             >  1:4503844/1‑93 (MQ=255)
                acacGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATa                                                                             >  2:4818964/1‑93 (MQ=255)
                 cacGGCGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATaa                                                                            <  1:2933693/93‑1 (MQ=255)
                 cacGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATaa                                                                            <  1:3722035/93‑1 (MQ=255)
                  acgacgATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAAt                                                                           <  2:3778970/93‑1 (MQ=255)
                      cgaTGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTaa                                                                       <  2:1263011/93‑1 (MQ=255)
                       gaTGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAg                                                                      >  2:5455542/1‑93 (MQ=255)
                          gTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGaa                                                                   >  1:5024866/1‑93 (MQ=255)
                          gTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGaa                                                                   >  1:4874260/1‑93 (MQ=255)
                           ttCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAAt                                                                  <  1:2319906/93‑1 (MQ=255)
                            tCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATg                                                                 <  2:2748760/93‑1 (MQ=255)
                             ccGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGc                                                                >  2:5912519/1‑93 (MQ=255)
                              cGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCg                                                               <  1:4588415/93‑1 (MQ=255)
                               ggCCAACGGCTTAATAGCGCCTGCAATGCACGGGCTGTTGTCCTTTCCCCCACCGCGACGCCGCCAGCAATACTGATAATGTAAGGAATGCGt                                                              <  2:4608267/93‑1 (MQ=255)
                                gCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGtt                                                             >  1:4056512/1‑93 (MQ=255)
                                gCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGtt                                                             <  2:491649/93‑1 (MQ=255)
                                gCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGtt                                                             >  1:4812404/1‑93 (MQ=255)
                                 ccAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTg                                                            >  1:5905661/1‑93 (MQ=255)
                                 ccAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTg                                                            >  1:1232318/1‑93 (MQ=255)
                                   aaCGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGcc                                                          >  2:2190791/1‑93 (MQ=255)
                                   aaCGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGcc                                                          >  1:2849546/1‑93 (MQ=255)
                                   aaCGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGcc                                                          >  2:5377154/1‑93 (MQ=255)
                                    aCGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGccc                                                         >  1:3284337/1‑93 (MQ=255)
                                    aCGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGccc                                                         <  1:1683300/93‑1 (MQ=255)
                                    aCGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGccc                                                         <  2:894215/93‑1 (MQ=255)
                                     cGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCg                                                        >  1:5619790/1‑93 (MQ=255)
                                      ggCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACGGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGt                                                       >  2:671745/1‑93 (MQ=255)
                                      ggCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGt                                                       >  2:4127009/1‑93 (MQ=255)
                                         ttAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTgg                                                    >  2:2038371/1‑93 (MQ=255)
                                         ttAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTgg                                                    >  1:1397001/1‑93 (MQ=255)
                                            aTAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTAc                                                 >  1:3356836/1‑93 (MQ=255)
                                             tAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTAcc                                                <  1:428065/93‑1 (MQ=255)
                                              aGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCa                                               <  2:4653101/93‑1 (MQ=255)
                                                 gcCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGa                                            <  2:1219887/93‑1 (MQ=255)
                                                 gcCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGa                                            <  1:3934097/93‑1 (MQ=255)
                                                    tGCAATACACGGGCGGTTGTACTTTCCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAAc                                         <  1:1582547/93‑1 (MQ=255)
                                                     gCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACt                                        >  1:1629419/1‑93 (MQ=255)
                                                     gCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACt                                        >  1:3023935/1‑93 (MQ=255)
                                                      cAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTg                                       >  2:3593876/1‑93 (MQ=255)
                                                       aaTACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGt                                      >  1:2177500/1‑93 (MQ=255)
                                                       aaTACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAATTGt                                      >  1:6064697/1‑93 (MQ=255)
                                                       aaTACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGt                                      >  1:6528604/1‑93 (MQ=255)
                                                        aTACACGGGCGTTTGTGCTTTTCCCCACCGCGCCACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGtt                                     <  1:2606473/93‑1 (MQ=255)
                                                         tACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTc                                    >  2:674787/1‑93 (MQ=255)
                                                         tACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTc                                    >  2:1587805/1‑93 (MQ=255)
                                                          acacGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTcc                                   >  2:5614482/1‑93 (MQ=255)
                                                          acacGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTcc                                   >  2:2629125/1‑93 (MQ=255)
                                                           cacGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCa                                  >  2:2658939/1‑93 (MQ=255)
                                                            acGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAg                                 >  2:1168240/1‑93 (MQ=255)
                                                             cGGGCGGTTGTACTTTTCCCAACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGa                                >  1:3776712/1‑93 (MQ=255)
                                                               ggCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAAc                              >  1:3685968/1‑93 (MQ=255)
                                                               ggCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAAc                              <  1:6109125/93‑1 (MQ=255)
                                                               ggCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAAc                              >  1:6261421/1‑93 (MQ=255)
                                                               ggCGGTTGTACTTTTCCCCACCACGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAAc                              <  2:5682202/93‑1 (MQ=255)
                                                                gCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACt                             <  2:1617182/93‑1 (MQ=255)
                                                                 cGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTg                            >  2:5787039/1‑93 (MQ=255)
                                                                  ggTTGTACTTTTCCCCCCCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGc                           <  2:4419440/93‑1 (MQ=255)
                                                                   gTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGcc                          >  2:338242/1‑93 (MQ=255)
                                                                    ttGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCt                         <  2:1389153/93‑1 (MQ=255)
                                                                    ttGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCt                         <  1:5749722/93‑1 (MQ=255)
                                                                    ttGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCt                         >  2:6518795/1‑93 (MQ=255)
                                                                     tGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTg                        >  1:5111401/1‑93 (MQ=255)
                                                                      gTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGa                       >  2:6035464/1‑93 (MQ=255)
                                                                       tACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGAc                      <  2:5139134/93‑1 (MQ=255)
                                                                         cTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACgg                    <  1:6346168/93‑1 (MQ=255)
                                                                         cTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACgg                    >  2:3037122/1‑93 (MQ=255)
                                                                          ttttCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGgc                   <  1:4432189/93‑1 (MQ=255)
                                                                          ttttCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGgc                   <  2:3443104/93‑1 (MQ=255)
                                                                          ttttCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGgc                   >  2:2906050/1‑93 (MQ=255)
                                                                           tttCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGgcg                  >  2:1130253/1‑93 (MQ=255)
                                                                            ttCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGgcgc                 >  1:3440720/1‑93 (MQ=255)
                                                                            ttCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGgcgc                 <  1:774598/93‑1 (MQ=255)
                                                                            ttCCCCACCGCGACACCGCCAGCAACACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGgccc                 <  2:6423055/93‑3 (MQ=255)
                                                                             tCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCa                <  1:581237/93‑1 (MQ=255)
                                                                               cccACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGa              <  1:2657969/93‑1 (MQ=255)
                                                                               cccACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGa              <  2:1874759/93‑1 (MQ=255)
                                                                               cccACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGa              >  1:2420033/1‑93 (MQ=255)
                                                                                ccACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGAt             >  2:4559270/1‑93 (MQ=255)
                                                                                 cACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGAtt            <  1:5327405/93‑1 (MQ=255)
                                                                                     gcgACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGACACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAg        >  2:176879/1‑93 (MQ=255)
                                                                                     gcgACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAg        >  1:2971887/1‑93 (MQ=255)
                                                                                     gcgACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAg        >  2:4211481/1‑93 (MQ=255)
                                                                                      cgACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGc       >  1:11596/1‑93 (MQ=255)
                                                                                       gACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCt      <  1:1947340/93‑1 (MQ=255)
                                                                                        acacCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCtt     <  1:904733/93‑1 (MQ=255)
                                                                                         cacCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTta    >  1:1003597/1‑93 (MQ=255)
                                                                                         cacCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTta    <  2:521673/93‑1 (MQ=255)
                                                                                            |                                                                                           
TGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATA  >  NC_000913/4172228‑4172411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATATA  >  NC_000913/4172227‑4172413
                                                                                             |                                                                                             
CTGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACT                                                                                               <  SRR4302547.1810629/94‑1 (MQ=60)
CTGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACC                                                                                               <  SRR4302547.2605918/94‑1 (MQ=60)
 TGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTG                                                                                              <  SRR4302547.1008095/94‑1 (MQ=60)
 TGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCG                                                                                              <  SRR4302547.6241878/94‑1 (MQ=60)
 TGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCG                                                                                              >  SRR4302547.5785788/1‑94 (MQ=60)
    AGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCA                                                                                           <  SRR4302547.558728/94‑1 (MQ=60)
      TGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGC                                                                                         <  SRR4302547.1065575/94‑1 (MQ=60)
      TGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGC                                                                                         >  SRR4302547.1796093/1‑94 (MQ=60)
      TGATCAGTTCAAAACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGC                                                                                         >  SRR4302547.1782317/1‑94 (MQ=60)
        ATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAA                                                                                       >  SRR4302547.4270506/1‑94 (MQ=60)
           AGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATAC                                                                                    >  SRR4302547.2164103/1‑94 (MQ=60)
           AGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATAC                                                                                    >  SRR4302547.1968495/1‑94 (MQ=60)
            GTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACT                                                                                   <  SRR4302547.3980726/94‑1 (MQ=60)
            GTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACT                                                                                   >  SRR4302547.5132212/1‑94 (MQ=60)
              TCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGA                                                                                 <  SRR4302547.40883/94‑1 (MQ=60)
                AACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATA                                                                               >  SRR4302547.4596846/1‑94 (MQ=60)
                  CACGGCGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAAT                                                                             <  SRR4302547.2992155/94‑1 (MQ=60)
                  CACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAAT                                                                             <  SRR4302547.3798013/94‑1 (MQ=60)
                   ACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATG                                                                            >  SRR4302547.5501510/1‑94 (MQ=60)
                   ACGACGATGTTCCGCCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACCCCGCCAGCAATACTGATAATG                                                                            <  SRR4302547.3106744/94‑1 (MQ=60)
                     GACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTA                                                                          >  SRR4302547.4352742/1‑94 (MQ=60)
                      ACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAA                                                                         <  SRR4302547.5728780/94‑1 (MQ=60)
                          TGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAT                                                                     >  SRR4302547.36470/1‑94 (MQ=60)
                          TGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAA                                                                     >  SRR4302547.5128686/1‑94 (MQ=60)
                          TGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAA                                                                     >  SRR4302547.4975260/1‑94 (MQ=60)
                           GTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAAT                                                                    <  SRR4302547.2921279/94‑1 (MQ=60)
                            TTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATG                                                                   <  SRR4302547.2366920/94‑1 (MQ=60)
                             TCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGC                                                                  <  SRR4302547.662007/94‑1 (MQ=60)
                             TCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGC                                                                  <  SRR4302547.3670160/94‑1 (MQ=60)
                               CGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGT                                                                <  SRR4302547.4683385/94‑1 (MQ=60)
                                GGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTT                                                               >  SRR4302547.6180488/1‑94 (MQ=60)
                                GGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTT                                                               <  SRR4302547.1047026/94‑1 (MQ=60)
                                GGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTT                                                               >  SRR4302547.4912146/1‑94 (MQ=60)
                                GGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTT                                                               >  SRR4302547.1346855/1‑94 (MQ=60)
                                GGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTT                                                               >  SRR4302547.4138886/1‑94 (MQ=60)
                                 GCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTG                                                              <  SRR4302547.5115142/94‑1 (MQ=60)
                                 GCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTG                                                              >  SRR4302547.1257177/1‑94 (MQ=60)
                                 GCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTG                                                              >  SRR4302547.2578710/1‑94 (MQ=60)
                                 GCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTG                                                              >  SRR4302547.6027968/1‑94 (MQ=60)
                                  CCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGC                                                             <  SRR4302547.5802871/94‑1 (MQ=60)
                                   CAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCC                                                            >  SRR4302547.4387259/1‑94 (MQ=60)
                                   CAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCC                                                            >  SRR4302547.999978/1‑94 (MQ=60)
                                   CAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCC                                                            >  SRR4302547.2906477/1‑94 (MQ=60)
                                    AACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCC                                                           <  SRR4302547.3081591/94‑1 (MQ=60)
                                    AACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCC                                                           >  SRR4302547.2215625/1‑94 (MQ=60)
                                    AACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCC                                                           >  SRR4302547.3350209/1‑94 (MQ=60)
                                     ACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCG                                                          <  SRR4302547.6391864/94‑1 (MQ=60)
                                     ACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCG                                                          <  SRR4302547.1716437/94‑1 (MQ=60)
                                     ACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCG                                                          >  SRR4302547.5735768/1‑94 (MQ=60)
                                      CGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGT                                                         >  SRR4302547.3796459/1‑94 (MQ=60)
                                       GGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTT                                                        >  SRR4302547.4876823/1‑94 (MQ=60)
                                       GGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTT                                                        >  SRR4302547.122940/1‑94 (MQ=60)
                                       GGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTT                                                        >  SRR4302547.69725/1‑94 (MQ=60)
                                       GGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTT                                                        >  SRR4302547.3437814/1‑94 (MQ=60)
                                         CTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGG                                                      >  SRR4302547.1424950/1‑94 (MQ=60)
                                          TTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGT                                                     >  SRR4302547.4452366/1‑94 (MQ=60)
                                          TTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGT                                                     <  SRR4302547.986675/94‑1 (MQ=60)
                                           TAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTA                                                    <  SRR4302547.3806336/94‑1 (MQ=60)
                                           TAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGGAAGGAATGCGTTGCGCGTTGttg                                                    >  SRR4302547.3370202/1‑91 (MQ=60)
                                            AATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTAC                                                   >  SRR4302547.3424925/1‑94 (MQ=60)
                                             ATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACC                                                  >  SRR4302547.1598641/1‑94 (MQ=60)
                                              TAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCA                                                 <  SRR4302547.435988/94‑1 (MQ=60)
                                                  GCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAA                                             <  SRR4302547.4014107/94‑1 (MQ=60)
                                                   CCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAA                                            >  SRR4302547.3264409/1‑94 (MQ=60)
                                                   CCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGCTGCCCGTTGGTACCAAGAAA                                            >  SRR4302547.855548/1‑94 (MQ=60)
                                                     TGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACT                                          >  SRR4302547.3084061/1‑94 (MQ=60)
                                                     TGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACT                                          >  SRR4302547.6376739/1‑94 (MQ=60)
                                                     TGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACT                                          >  SRR4302547.1661673/1‑94 (MQ=60)
                                                     TGCAATACACGGGCGGTTGTACTTTCCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACT                                          <  SRR4302547.1614031/94‑1 (MQ=60)
                                                      GCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTG                                         <  SRR4302547.3071252/94‑1 (MQ=60)
                                                      GCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTG                                         >  SRR4302547.5047277/1‑94 (MQ=60)
                                                       CAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGT                                        >  SRR4302547.2221104/1‑94 (MQ=60)
                                                       CAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAATTGT                                        >  SRR4302547.6190496/1‑94 (MQ=60)
                                                       CAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGT                                        >  SRR4302547.6664496/1‑94 (MQ=60)
                                                       CAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGT                                        >  SRR4302547.206068/1‑94 (MQ=60)
                                                       CAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGT                                        >  SRR4302547.6198078/1‑94 (MQ=60)
                                                        AATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTT                                       <  SRR4302547.1690106/94‑1 (MQ=60)
                                                        AATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGGTGGTACCAAGAAACTGTT                                       >  SRR4302547.2337583/1‑94 (MQ=60)
                                                         ATACACGGGCGTTTGTGCTTTTCCCCACCGCGCCACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTC                                      <  SRR4302547.2658998/94‑1 (MQ=60)
                                                         ATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTC                                      >  SRR4302547.2457135/1‑94 (MQ=60)
                                                         ATACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTC                                      >  SRR4302547.589704/1‑94 (MQ=60)
                                                          TACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCC                                     <  SRR4302547.1435767/94‑1 (MQ=60)
                                                           ACACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCA                                    <  SRR4302547.704931/94‑1 (MQ=60)
                                                            CACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAG                                   >  SRR4302547.4806706/1‑94 (MQ=60)
                                                            CACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAG                                   <  SRR4302547.5942691/94‑1 (MQ=60)
                                                            CACGGGCGGTAGTACTTTTCCCCACCGAGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAG                                   <  SRR4302547.5460042/94‑1 (MQ=60)
                                                             ACGGTCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTTCCAAGAAACTGTTACAGA                                  >  SRR4302547.3689678/1‑94 (MQ=60)
                                                             ACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGA                                  <  SRR4302547.2264555/94‑1 (MQ=60)
                                                             ACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGA                                  <  SRR4302547.2665686/94‑1 (MQ=60)
                                                             ACGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGA                                  >  SRR4302547.1143444/1‑94 (MQ=60)
                                                             ACGGGCGGTTGTACTTTTCCCAACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGA                                  >  SRR4302547.3853734/1‑94 (MQ=60)
                                                              CGGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAA                                 >  SRR4302547.165288/1‑94 (MQ=60)
                                                               GGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAAC                                >  SRR4302547.3761220/1‑94 (MQ=60)
                                                               GGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAAC                                >  SRR4302547.6391502/1‑94 (MQ=60)
                                                               GGGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAAC                                >  SRR4302547.5776613/1‑94 (MQ=60)
                                                                GGCGGTTGTTCTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACT                               <  SRR4302547.6362012/94‑1 (MQ=60)
                                                                GGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACT                               >  SRR4302547.4633765/1‑94 (MQ=60)
                                                                GGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACT                               <  SRR4302547.2557708/94‑1 (MQ=60)
                                                                GGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACT                               <  SRR4302547.3799153/94‑1 (MQ=60)
                                                                GGCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACT                               <  SRR4302547.6235841/94‑1 (MQ=60)
                                                                 GCGGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTTCCAAGAAACTGTTCCAGAACTG                              >  SRR4302547.1700191/1‑94 (MQ=60)
                                                                   GGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCC                            >  SRR4302547.2785395/1‑94 (MQ=60)
                                                                   GGTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCC                            <  SRR4302547.291339/94‑1 (MQ=60)
                                                                    GTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCT                           <  SRR4302547.4136224/94‑1 (MQ=60)
                                                                    GTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCT                           >  SRR4302547.2004187/1‑94 (MQ=60)
                                                                    GTTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCT                           <  SRR4302547.6095079/94‑1 (MQ=60)
                                                                     TTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTG                          >  SRR4302547.464310/1‑94 (MQ=60)
                                                                     TTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTG                          <  SRR4302547.5868567/94‑1 (MQ=60)
                                                                     TTGTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTG                          >  SRR4302547.5216733/1‑94 (MQ=60)
                                                                       GTACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGAC                        >  SRR4302547.175895/1‑94 (MQ=60)
                                                                        TACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACG                       <  SRR4302547.3003610/94‑1 (MQ=60)
                                                                        TACTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCCGACG                       >  SRR4302547.1314048/1‑94 (MQ=60)
                                                                          CTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGC                     >  SRR4302547.5306561/1‑94 (MQ=60)
                                                                          CTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGC                     <  SRR4302547.6478361/94‑1 (MQ=60)
                                                                          CTTTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGC                     >  SRR4302547.2710224/1‑94 (MQ=60)
                                                                           TTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCG                    <  SRR4302547.4523808/94‑1 (MQ=60)
                                                                            TTTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGC                   >  SRR4302547.3510867/1‑94 (MQ=60)
                                                                             TTCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCA                  <  SRR4302547.789243/94‑1 (MQ=60)
                                                                              TCCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAG                 <  SRR4302547.591669/94‑1 (MQ=60)
                                                                              TCCCCACCGCGACACCGCCAGCAACACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAG                 <  SRR4302547.4578386/94‑1 (MQ=60)
                                                                               CCCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGA                >  SRR4302547.2469023/1‑94 (MQ=60)
                                                                                CCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGAT               >  SRR4302547.1101680/1‑94 (MQ=60)
                                                                                CCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGAT               <  SRR4302547.2711383/94‑1 (MQ=60)
                                                                                CCCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGAT               >  SRR4302547.3668137/1‑94 (MQ=60)
                                                                                 CCACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATT              >  SRR4302547.5869860/1‑94 (MQ=60)
                                                                                  CACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTC             >  SRR4302547.4922521/1‑94 (MQ=60)
                                                                                  CACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTC             <  SRR4302547.5437203/94‑1 (MQ=60)
                                                                                  CACCGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTC             <  SRR4302547.6462468/94‑1 (MQ=60)
                                                                                     CGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAG          <  SRR4302547.3425768/94‑1 (MQ=60)
                                                                                     CGCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAG          >  SRR4302547.3031030/1‑94 (MQ=60)
                                                                                      GCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGC         >  SRR4302547.4255732/1‑94 (MQ=60)
                                                                                      GCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGC         >  SRR4302547.11809/1‑94 (MQ=60)
                                                                                      GCGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGC         >  SRR4302547.4150670/1‑94 (MQ=60)
                                                                                       CGACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCT        <  SRR4302547.5359352/94‑1 (MQ=60)
                                                                                        GACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTT       <  SRR4302547.1985945/94‑1 (MQ=60)
                                                                                         ACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTA      >  SRR4302547.1023176/1‑94 (MQ=60)
                                                                                         ACACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTA      <  SRR4302547.922161/94‑1 (MQ=60)
                                                                                          CACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTAT     >  SRR4302547.6242124/1‑94 (MQ=60)
                                                                                          CACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTAT     >  SRR4302547.3302418/1‑94 (MQ=60)
                                                                                           ACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATA    <  SRR4302547.3801243/94‑1 (MQ=60)
                                                                                           ACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATA    >  SRR4302547.5238617/1‑94 (MQ=60)
                                                                                           ACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATA    >  SRR4302547.5504303/1‑94 (MQ=60)
                                                                                           ACCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGGTGGTACCAAGAAACTGTTCCAGAACTGGCTGGCGGCGCAGATTCGAGCTTATT    >  SRR4302547.2988763/1‑94 (MQ=60)
                                                                                            CCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATAT   <  SRR4302547.6784271/94‑1 (MQ=60)
                                                                                            CCGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATAT   <  SRR4302547.4217707/94‑1 (MQ=60)
                                                                                             CGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATATA  >  SRR4302547.4098307/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGTAGTGATCAGTTCAACACGACGATGTTCCGGCCAACGGCTTAATAGCGCCTGCAATACACGGGCGGTTGTACTTTTCCCCACCGCGACACTGCCAGCAATACTGATAATGTAAGGAATGCGTTGCCCGTTGGTACCAAGAAACTGTTCCAGAACTGCCTGACGGCGCAGATTCGAGCTTATATA  >  NC_000913/4172227‑4172413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑