Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,175,751 T→G F144V (TTC→GTC)  nusG → NusG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,7510TG99.2% 859.1 / ‑4.9 235F144V (TTC→GTC) nusGNusG
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (100/133);  total (101/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG  >  NC_000913/4175661‑4175840
                                                                                          |                                                                                         
 gCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCa                                                                                        <  2:4549408/93‑1 (MQ=255)
 gCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCa                                                                                        >  1:2122740/1‑93 (MQ=255)
  ccTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                       >  1:1539361/1‑93 (MQ=255)
  ccTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                       <  1:5398959/93‑1 (MQ=255)
  ccTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                       <  1:5164504/93‑1 (MQ=255)
   cTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAc                                                                                      >  2:2288823/1‑93 (MQ=255)
    tGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACg                                                                                     <  2:3075226/93‑1 (MQ=255)
    tGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGCGAAATGGTCCGTGTTAATGATGGTCCGTTAGCTGACGTCAACg                                                                                     >  2:1969613/1‑93 (MQ=255)
     gcagcaGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACgg                                                                                    <  1:4255749/93‑1 (MQ=255)
     gcagcaGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACgg                                                                                    <  2:4617838/93‑1 (MQ=255)
      cagcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgt                                                                                   <  2:1824746/93‑1 (MQ=255)
        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 <  1:4399119/93‑1 (MQ=255)
        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 >  1:4788359/1‑93 (MQ=255)
        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 <  1:4695024/93‑1 (MQ=255)
        gcagGTTGGTGATAAGCCGCGCCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 >  1:5878603/1‑93 (MQ=255)
         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                >  1:5024592/1‑93 (MQ=255)
         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                <  1:5603734/93‑1 (MQ=255)
         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                >  2:2638838/1‑93 (MQ=255)
         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                <  1:800688/93‑1 (MQ=255)
          agGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttg                                                                               <  1:3586700/93‑1 (MQ=255)
           ggTTGGTGATAATCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              <  1:1011113/93‑1 (MQ=255)
           ggTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              >  2:168435/1‑93 (MQ=255)
           ggTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              <  1:2939800/93‑1 (MQ=255)
            gTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTgttgtt                                                                             >  1:1136397/1‑93 (MQ=255)
              tGGTGATAAGCCTCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                           <  1:2426816/93‑1 (MQ=255)
               ggTGATAAGCCGCGTCCGAAAGCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaa                                                                          <  1:3257607/93‑1 (MQ=255)
               ggTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaa                                                                          >  2:3826943/1‑93 (MQ=255)
               ggTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGATGTTGATgaa                                                                          >  1:1355549/1‑93 (MQ=255)
                   aTAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGaagaag                                                                      >  2:1043107/1‑93 (MQ=255)
                   aTAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGaagaag                                                                      >  2:5195264/1‑93 (MQ=255)
                    tAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGaagaagg                                                                     >  1:2266700/1‑92 (MQ=255)
                       gcagcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGa                                                                  <  1:6074302/90‑1 (MQ=255)
                       gCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGa                                                                  <  2:3637208/93‑1 (MQ=255)
                        ccGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGAt                                                                 >  2:5792392/1‑93 (MQ=255)
                        ccGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGAt                                                                 <  1:1662958/93‑1 (MQ=255)
                          gcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTa                                                               <  1:780678/93‑1 (MQ=255)
                          gcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTa                                                               >  2:533161/1‑93 (MQ=255)
                           cgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAc                                                              <  1:3018390/93‑1 (MQ=255)
                            gTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACg                                                             <  1:1220700/93‑1 (MQ=255)
                            gTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACg                                                             >  2:5213297/1‑93 (MQ=255)
                             tCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACga                                                            >  1:99431/1‑93 (MQ=255)
                                gAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaa                                                         >  2:4365185/1‑93 (MQ=255)
                                 aaaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaaa                                                        >  2:2777463/1‑93 (MQ=255)
                                 aaaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaaa                                                        <  1:2724220/93‑1 (MQ=255)
                                  aaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAt                                                       <  1:662849/93‑1 (MQ=255)
                                  aaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAt                                                       >  2:5107843/1‑93 (MQ=255)
                                  aaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAt                                                       >  2:2344880/1‑93 (MQ=255)
                                   aaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCTCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtc                                                      <  1:5298573/93‑1 (MQ=255)
                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     <  2:2796067/93‑1 (MQ=255)
                                      gCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCg                                                   <  1:5191531/93‑1 (MQ=255)
                                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGt                                                  <  2:2591167/93‑1 (MQ=255)
                                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGt                                                  <  2:294299/93‑1 (MQ=255)
                                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGt                                                  <  1:1120907/93‑1 (MQ=255)
                                         gTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCt                                                >  1:714623/1‑93 (MQ=255)
                                              aaCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAg                                           <  1:5661073/93‑1 (MQ=255)
                                               aCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgt                                          <  2:925058/93‑1 (MQ=255)
                                                ccGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgtg                                         <  1:450735/93‑1 (MQ=255)
                                                 cGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgtgt                                        >  1:4981968/1‑93 (MQ=255)
                                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                       <  2:5185709/93‑1 (MQ=255)
                                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                       <  2:3301093/93‑1 (MQ=255)
                                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                       >  2:1459031/1‑93 (MQ=255)
                                                   ggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCt                                      <  2:407947/93‑1 (MQ=255)
                                                      gAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGtt                                   <  2:5399648/93‑1 (MQ=255)
                                                        aaTGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTc                                 >  2:632729/1‑93 (MQ=255)
                                                        aaTGGTCCGTGCTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTc                                 <  1:4635874/93‑1 (MQ=255)
                                                         aTGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCt                                <  1:4041626/93‑1 (MQ=255)
                                                          tGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTa                               <  1:2565700/93‑1 (MQ=255)
                                                          tGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGACTGAAAGTGTCTGTTTCTa                               >  2:5988651/1‑93 (MQ=255)
                                                           tgTCCGTGTTAATGGTGGTCCGTTCGCTGACGTCAACGGTTTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAt                              <  1:3645670/92‑1 (MQ=255)
                                                            gTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATc                             <  1:3745443/93‑1 (MQ=255)
                                                             tCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCt                            <  1:3552279/93‑1 (MQ=255)
                                                               cGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTc                          <  1:1775613/93‑1 (MQ=255)
                                                               cGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTc                          <  1:6110084/93‑1 (MQ=255)
                                                                gtgtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAGGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCg                         >  1:4362142/1‑93 (MQ=255)
                                                                gtgtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCg                         >  1:1541102/1‑93 (MQ=255)
                                                                gtgtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCg                         >  1:3888845/1‑93 (MQ=255)
                                                                    tAATGATGGTCAGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCg                     <  1:4533998/93‑1 (MQ=255)
                                                                     aaTGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGt                    >  2:2087645/1‑93 (MQ=255)
                                                                       tgatgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGc                  <  1:1963343/93‑1 (MQ=255)
                                                                       tgatgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGc                  >  1:2507927/1‑93 (MQ=255)
                                                                         atgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGa                >  2:5143033/1‑93 (MQ=255)
                                                                          tgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAc               <  1:4000214/93‑1 (MQ=255)
                                                                           ggcccGTTCGCTGACGTCCACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcc              >  1:3772795/4‑93 (MQ=255)
                                                                           ggTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcc              >  2:5888947/1‑93 (MQ=255)
                                                                            gTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAccc             <  2:3418710/93‑1 (MQ=255)
                                                                             tCCGTTCGCTGATGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcccc            <  1:3959115/93‑1 (MQ=255)
                                                                             tCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcccc            >  2:1149258/1‑93 (MQ=255)
                                                                               cGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCgg          >  1:3210826/1‑93 (MQ=255)
                                                                                gTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGt         <  2:3543241/93‑1 (MQ=255)
                                                                                 ttCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTa        >  2:4913998/1‑93 (MQ=255)
                                                                                 ttCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTa        >  1:4564257/1‑93 (MQ=255)
                                                                                  tCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag       <  2:3947406/93‑1 (MQ=255)
                                                                                  tCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag       >  2:5999172/1‑93 (MQ=255)
                                                                                     cTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc    <  1:5754962/93‑1 (MQ=255)
                                                                                     cTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc    <  1:5997138/93‑1 (MQ=255)
                                                                                      tGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCt   <  1:2025334/93‑1 (MQ=255)
                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  <  2:4865851/93‑1 (MQ=255)
                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  >  2:4725615/1‑93 (MQ=255)
                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  >  1:2632587/1‑93 (MQ=255)
                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  >  1:915993/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG  >  NC_000913/4175661‑4175840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  NC_000913/4175658‑4175844
                                                                                             |                                                                                             
AACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACG                                                                                               <  SRR4302532.5994254/94‑1 (MQ=60)
 ACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGT                                                                                              >  SRR4302532.926507/1‑94 (MQ=60)
 ACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGT                                                                                              <  SRR4302532.4708844/94‑1 (MQ=60)
 ACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGT                                                                                              >  SRR4302532.4041980/1‑94 (MQ=60)
  CCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTC                                                                                             <  SRR4302532.5533927/94‑1 (MQ=60)
  CCGCCTGCAGCAGGTAGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTC                                                                                             <  SRR4302532.5660432/94‑1 (MQ=60)
   CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGTTGAAATGGGCCGTGGTAATGATGGTCCGTTCGCTGACGTCA                                                                                            >  SRR4302532.4121656/1‑94 (MQ=60)
   CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCA                                                                                            >  SRR4302532.2193653/1‑94 (MQ=60)
    GCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAA                                                                                           >  SRR4302532.1591366/1‑94 (MQ=60)
    GCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAA                                                                                           >  SRR4302532.1591367/1‑94 (MQ=60)
     CCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAC                                                                                          <  SRR4302532.5342290/94‑1 (MQ=60)
     CCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAC                                                                                          <  SRR4302532.1895491/94‑1 (MQ=60)
     CCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAC                                                                                          <  SRR4302532.5586034/94‑1 (MQ=60)
      CTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACG                                                                                         >  SRR4302532.4726528/1‑94 (MQ=60)
      CTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACG                                                                                         <  SRR4302532.5161444/94‑1 (MQ=60)
      CTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGCCAACG                                                                                         >  SRR4302532.4173763/1‑94 (MQ=60)
       TGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGG                                                                                        >  SRR4302532.3814339/1‑94 (MQ=60)
       TGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGG                                                                                        <  SRR4302532.2959609/94‑1 (MQ=60)
        GCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGT                                                                                       <  SRR4302532.2290519/94‑1 (MQ=60)
        GCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGT                                                                                       <  SRR4302532.4398852/94‑1 (MQ=60)
         CAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTG                                                                                      <  SRR4302532.5878590/94‑1 (MQ=60)
         CAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTG                                                                                      <  SRR4302532.1160064/94‑1 (MQ=60)
          AGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGT                                                                                     >  SRR4302532.4953243/1‑94 (MQ=60)
          AGCAGGTTGGTGATAAGCCGCGCCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGT                                                                                     >  SRR4302532.6082240/1‑94 (MQ=60)
           GCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTT                                                                                    >  SRR4302532.1769311/1‑94 (MQ=60)
           GCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTT                                                                                    >  SRR4302532.5196926/1‑94 (MQ=60)
           GCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTT                                                                                    <  SRR4302532.4548846/94‑1 (MQ=60)
           GCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTT                                                                                    <  SRR4302532.4855157/94‑1 (MQ=60)
           GCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTT                                                                                    <  SRR4302532.4855158/94‑1 (MQ=60)
            CAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTG                                                                                   <  SRR4302532.828052/94‑1 (MQ=60)
            CAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTG                                                                                   <  SRR4302532.1302223/94‑1 (MQ=60)
            CAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTG                                                                                   <  SRR4302532.5798245/94‑1 (MQ=60)
             AGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGT                                                                                  <  SRR4302532.3706683/94‑1 (MQ=60)
              GGTTGGTGATAATCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTT                                                                                 <  SRR4302532.1045481/94‑1 (MQ=60)
              GGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTT                                                                                 <  SRR4302532.4530236/94‑1 (MQ=60)
              GGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTT                                                                                 >  SRR4302532.1174304/1‑94 (MQ=60)
              GGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTT                                                                                 <  SRR4302532.6360/94‑1 (MQ=60)
              GGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTT                                                                                 <  SRR4302532.3037434/94‑1 (MQ=60)
              GGTGGGTGAGAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTT                                                                                 <  SRR4302532.4530235/94‑1 (MQ=60)
               GTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTG                                                                                <  SRR4302532.5829666/94‑1 (MQ=60)
               GTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTG                                                                                <  SRR4302532.706542/94‑1 (MQ=60)
                 TGGTGATAAGCCTCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              <  SRR4302532.2507468/94‑1 (MQ=60)
                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              >  SRR4302532.312310/1‑94 (MQ=60)
                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGATGTTGATGAA                                                                              >  SRR4302532.1400907/1‑94 (MQ=60)
                  GGTGATAAGCCGCGTCCGAAAGCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAG                                                                             <  SRR4302532.3365846/94‑1 (MQ=60)
                     GATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          <  SRR4302532.2063160/94‑1 (MQ=60)
                     GATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          <  SRR4302532.140785/94‑1 (MQ=60)
                     GATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          <  SRR4302532.3726465/94‑1 (MQ=60)
                     GATAAGCCGCGTCCAAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          <  SRR4302532.627615/94‑1 (MQ=60)
                      ATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGG                                                                         >  SRR4302532.2341928/1‑94 (MQ=60)
                        AAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGG                                                                       <  SRR4302532.2712619/94‑1 (MQ=60)
                         AGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGA                                                                      >  SRR4302532.2798749/1‑94 (MQ=60)
                         AGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGA                                                                      <  SRR4302532.3621462/94‑1 (MQ=60)
                          GCAGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGAT                                                                     <  SRR4302532.6284076/94‑1 (MQ=60)
                           CCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATT                                                                    <  SRR4302532.1719291/94‑1 (MQ=60)
                             GCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAC                                                                  <  SRR4302532.807523/94‑1 (MQ=60)
                             GCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAC                                                                  <  SRR4302532.807524/94‑1 (MQ=60)
                              CGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACG                                                                 >  SRR4302532.546353/1‑94 (MQ=60)
                              CGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACG                                                                 <  SRR4302532.3117976/94‑1 (MQ=60)
                               GTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGA                                                                >  SRR4302532.102883/1‑94 (MQ=60)
                               GTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGA                                                                <  SRR4302532.1261546/94‑1 (MQ=60)
                                 CCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGA                                                              <  SRR4302532.871677/94‑1 (MQ=60)
                                 CCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGA                                                              >  SRR4302532.167320/1‑94 (MQ=60)
                                 CCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGA                                                              <  SRR4302532.644133/94‑1 (MQ=60)
                                 CAGAAAACGCTGTTTGAACCTGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGTGGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGA                                                              <  SRR4302532.644134/94‑1 (MQ=60)
                                  CGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAA                                                             >  SRR4302532.6150921/1‑94 (MQ=60)
                                   GAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAA                                                            >  SRR4302532.484633/1‑94 (MQ=60)
                                    AAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAT                                                           <  SRR4302532.2815837/94‑1 (MQ=60)
                                    AAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAT                                                           <  SRR4302532.2638885/94‑1 (MQ=60)
                                     AAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATC                                                          <  SRR4302532.685770/94‑1 (MQ=60)
                                     AAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATC                                                          >  SRR4302532.1991054/1‑94 (MQ=60)
                                     AAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATC                                                          <  SRR4302532.5705037/94‑1 (MQ=60)
                                      AACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCTCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCT                                                         <  SRR4302532.5481608/94‑1 (MQ=60)
                                      AACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCT                                                         <  SRR4302532.3213539/94‑1 (MQ=60)
                                        CGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCG                                                       >  SRR4302532.5426440/1‑94 (MQ=60)
                                         GCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGT                                                      <  SRR4302532.3679285/94‑1 (MQ=60)
                                         GCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGT                                                      <  SRR4302532.5370457/94‑1 (MQ=60)
                                          CTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTC                                                     <  SRR4302532.1158335/94‑1 (MQ=60)
                                          CTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTC                                                     >  SRR4302532.510409/1‑94 (MQ=60)
                                          CTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTC                                                     <  SRR4302532.4687488/94‑1 (MQ=60)
                                          CTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTC                                                     >  SRR4302532.768104/1‑94 (MQ=60)
                                           TGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCT                                                    >  SRR4302532.739022/1‑94 (MQ=60)
                                           TGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCT                                                    <  SRR4302532.4074725/94‑1 (MQ=60)
                                            GTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTG                                                   <  SRR4302532.2561577/94‑1 (MQ=60)
                                            GTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTG                                                   >  SRR4302532.3102976/1‑94 (MQ=60)
                                              TTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAA                                                 <  SRR4302532.5302940/94‑1 (MQ=60)
                                                 AACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGT                                              <  SRR4302532.5858330/94‑1 (MQ=60)
                                                   CCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGT                                            >  SRR4302532.5152748/1‑94 (MQ=60)
                                                   CCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGT                                            <  SRR4302532.466386/94‑1 (MQ=60)
                                                   CCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGT                                            <  SRR4302532.1190630/94‑1 (MQ=60)
                                                    CGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTC                                           >  SRR4302532.4096985/1‑94 (MQ=60)
                                                    CGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTC                                           >  SRR4302532.1696091/1‑94 (MQ=60)
                                                      GGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTG                                         >  SRR4302532.2186010/1‑94 (MQ=60)
                                                      GGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTG                                         <  SRR4302532.5939402/94‑1 (MQ=60)
                                                      GGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTG                                         <  SRR4302532.5984443/94‑1 (MQ=60)
                                                      GGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTG                                         <  SRR4302532.917251/94‑1 (MQ=60)
                                                       GTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGT                                        >  SRR4302532.3877779/1‑94 (MQ=60)
                                                          AAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTC                                     >  SRR4302532.4704958/1‑94 (MQ=60)
                                                           AATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCT                                    <  SRR4302532.4677086/94‑1 (MQ=60)
                                                           AATGGTCCGTGCTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCT                                    <  SRR4302532.4793936/94‑1 (MQ=60)
                                                            ATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTA                                   <  SRR4302532.4177749/94‑1 (MQ=60)
                                                            ATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTA                                   >  SRR4302532.5531490/1‑94 (MQ=60)
                                                             TGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAT                                  <  SRR4302532.2651334/94‑1 (MQ=60)
                                                             TGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAT                                  <  SRR4302532.2659916/94‑1 (MQ=60)
                                                             TGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAT                                  >  SRR4302532.5294858/1‑94 (MQ=60)
                                                              TGTCCGTGTTAATGGTGGTCCGTTCGCTGACGTCAACGGTTTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATC                                 <  SRR4302532.3767997/94‑1 (MQ=60)
                                                               GTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCT                                <  SRR4302532.3871332/94‑1 (MQ=60)
                                                                TCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTT                               <  SRR4302532.3671123/94‑1 (MQ=60)
                                                                 CCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTC                              >  SRR4302532.5122475/1‑94 (MQ=60)
                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAGGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             >  SRR4302532.4509988/1‑94 (MQ=60)
                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             >  SRR4302532.4762615/1‑94 (MQ=60)
                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             >  SRR4302532.971891/1‑94 (MQ=60)
                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             >  SRR4302532.1593184/1‑94 (MQ=60)
                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             <  SRR4302532.1835068/94‑1 (MQ=60)
                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             <  SRR4302532.6320984/94‑1 (MQ=60)
                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             >  SRR4302532.4020062/1‑94 (MQ=60)
                                                                    TGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGT                           <  SRR4302532.76933/94‑1 (MQ=60)
                                                                     GTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTC                          <  SRR4302532.4197068/94‑1 (MQ=60)
                                                                       TAATGATGGTCAGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGT                        <  SRR4302532.4688746/94‑1 (MQ=60)
                                                                        AATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTG                       >  SRR4302532.1592897/1‑94 (MQ=60)
                                                                        AATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTG                       <  SRR4302532.5342707/94‑1 (MQ=60)
                                                                         ATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGC                      >  SRR4302532.6119207/1‑94 (MQ=60)
                                                                         ATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGC                      >  SRR4302532.2591585/1‑94 (MQ=60)
                                                                         ATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGC                      >  SRR4302532.3353606/1‑94 (MQ=60)
                                                                          TGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCG                     <  SRR4302532.1249576/94‑1 (MQ=60)
                                                                          TGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCG                     <  SRR4302532.2028549/94‑1 (MQ=60)
                                                                           GATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGC                    >  SRR4302532.4560082/1‑94 (MQ=60)
                                                                           GATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGA                    >  SRR4302532.1052017/1‑94 (MQ=60)
                                                                             TGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACC                  <  SRR4302532.4134970/94‑1 (MQ=60)
                                                                             TGGCCCGTTCGCTGACGTCCACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACC                  >  SRR4302532.3899821/1‑94 (MQ=60)
                                                                               GTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCC                <  SRR4302532.3558564/94‑1 (MQ=60)
                                                                                TCCGTTCGCTGATGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCG               <  SRR4302532.4092518/94‑1 (MQ=60)
                                                                                TCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCG               >  SRR4302532.2434130/1‑94 (MQ=60)
                                                                                 CCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGG              >  SRR4302532.3317436/1‑94 (MQ=60)
                                                                                   GTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGGTTACGAGAAATCTCGACTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTA            >  SRR4302532.4785083/1‑94 (MQ=60)
                                                                                   GTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTA            >  SRR4302532.4719944/1‑94 (MQ=60)
                                                                                   GTTCGCTGACGTCAACGGTGTTGTTAAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTA            >  SRR4302532.2556691/1‑94 (MQ=60)
                                                                                     TCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGA          <  SRR4302532.5742718/94‑1 (MQ=60)
                                                                                       GCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGC        >  SRR4302532.3969523/1‑94 (MQ=60)
                                                                                       GCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGC        >  SRR4302532.4381500/1‑94 (MQ=60)
                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       <  SRR4302532.6204295/94‑1 (MQ=60)
                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       <  SRR4302532.289323/94‑1 (MQ=60)
                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       <  SRR4302532.5955264/94‑1 (MQ=60)
                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       >  SRR4302532.6328093/1‑94 (MQ=60)
                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       <  SRR4302532.4445036/94‑1 (MQ=60)
                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       <  SRR4302532.2147913/94‑1 (MQ=60)
                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG      <  SRR4302532.2092857/94‑1 (MQ=60)
                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG      >  SRR4302532.3970505/1‑94 (MQ=60)
                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG      <  SRR4302532.374053/94‑1 (MQ=60)
                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG      >  SRR4302532.947329/1‑94 (MQ=60)
                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG      >  SRR4302532.2720788/1‑94 (MQ=60)
                                                                                           ACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGA    <  SRR4302532.1641090/94‑1 (MQ=60)
                                                                                            CGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGAC   >  SRR4302532.2016853/1‑94 (MQ=60)
                                                                                            CGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGAC   >  SRR4302532.3967010/1‑94 (MQ=60)
                                                                                             GTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  SRR4302532.450805/1‑94 (MQ=60)
                                                                                             GTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  SRR4302532.926473/1‑94 (MQ=60)
                                                                                             GTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  SRR4302532.1208066/1‑94 (MQ=60)
                                                                                             GTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  SRR4302532.453901/1‑94 (MQ=60)
                                                                                             GTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGGAGAGCTGGACT  >  SRR4302532.5490397/1‑94 (MQ=60)
                                                                                             |                                                                                             
AACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  NC_000913/4175658‑4175844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑