Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,751	T→G	F144V (TTC→GTC)	nusG →	NusG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,751	0	T	G	100.0%	1014.5 / NA	270	F144V (TTC→GTC)	nusG	NusG
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (127/143); total (127/143)

Only 100 of 272 total aligned reads displayed.

CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG  >  NC_000913/4175661‑4175840

cGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTc                                                                                         >  1:4060538/1‑93 (MQ=255)

 gCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCa                                                                                        <  2:1630857/93‑1 (MQ=255)

  ccTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCaa                                                                                       <  1:1988646/93‑1 (MQ=255)

   cTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAc                                                                                      <  2:2921510/93‑1 (MQ=255)

     gcagcaGGTCGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGATGTCAACgg                                                                                    <  1:6474033/93‑1 (MQ=255)

        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 >  1:1349838/1‑93 (MQ=255)

        gcagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGgtgt                                                                                 >  2:173292/1‑93 (MQ=255)

         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                >  2:3572690/1‑93 (MQ=255)

         cagGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgtt                                                                                <  1:880207/93‑1 (MQ=255)

          agGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttg                                                                               >  1:4946849/1‑93 (MQ=255)

           ggTTGGTGATAAGCCGCGTCGGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              <  2:4555119/93‑1 (MQ=255)

           ggTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              >  1:4723328/1‑93 (MQ=255)

           ggTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGtgttgt                                                                              <  2:2405070/93‑1 (MQ=255)

             ttGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGttgttg                                                                            >  2:5537850/1‑93 (MQ=255)

              tGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                           <  1:6134657/93‑1 (MQ=255)

              tGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                           <  1:6109195/93‑1 (MQ=255)

              tGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                           <  1:3299604/93‑1 (MQ=255)

              tGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTga                                                                           >  1:5687671/1‑93 (MQ=255)

                gTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaag                                                                         >  1:2814536/1‑93 (MQ=255)

                 tGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaaga                                                                        >  1:2423827/1‑93 (MQ=255)

                 tGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaaga                                                                        <  1:4408252/93‑1 (MQ=255)

                  gATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTgaagaa                                                                       <  2:3726550/93‑1 (MQ=255)

                   aTAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGaagaag                                                                      <  2:3887969/93‑1 (MQ=255)

                     aaGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTg                                                                    >  2:2797905/1‑93 (MQ=255)

                     aaGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTg                                                                    <  1:209399/93‑1 (MQ=255)

                       gCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGa                                                                  <  1:827189/93‑1 (MQ=255)

                           cgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAc                                                              <  2:2265116/93‑1 (MQ=255)

                             tCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAcaa                                                            <  1:6358874/93‑3 (MQ=255)

                             tCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACga                                                            >  2:1857983/1‑93 (MQ=255)

                             tCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACga                                                            >  2:2344613/1‑93 (MQ=255)

                                gAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaa                                                         >  1:712556/1‑93 (MQ=255)

                                gAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaa                                                         >  1:3452671/1‑93 (MQ=255)

                                 aaaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaaa                                                        <  2:3112692/93‑1 (MQ=255)

                                 aaaaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGaaa                                                        >  2:1170250/1‑93 (MQ=255)

                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     <  2:5630342/93‑1 (MQ=255)

                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     <  2:4246236/93‑1 (MQ=255)

                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     >  1:2309813/1‑93 (MQ=255)

                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     >  1:5874346/1‑93 (MQ=255)

                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     <  2:2437802/93‑1 (MQ=255)

                                    aCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtct                                                     >  2:2165603/1‑93 (MQ=255)

                                     cGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtctc                                                    >  2:2048439/1‑93 (MQ=255)

                                     cGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtctc                                                    <  1:5608439/93‑1 (MQ=255)

                                     cGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAAtctc                                                    >  2:1642777/1‑93 (MQ=255)

                                      gCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCg                                                   <  2:3928334/93‑1 (MQ=255)

                                        tGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTc                                                 >  1:2894987/1‑93 (MQ=255)

                                        tGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTc                                                 >  2:3425832/1‑93 (MQ=255)

                                          tttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTg                                               >  1:5694361/1‑93 (MQ=255)

                                          ttggAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTg                                               <  1:1075374/90‑1 (MQ=255)

                                           ttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGa                                              <  1:1391689/93‑1 (MQ=255)

                                             gagccGGGTGAAATGGTCAGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGaaa                                            <  1:2602844/90‑1 (MQ=255)

                                             gAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGaaa                                            <  1:1277658/93‑1 (MQ=255)

                                              aaCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAg                                           >  1:6040766/1‑93 (MQ=255)

                                              aaCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAg                                           >  2:4779660/1‑93 (MQ=255)

                                              aaCCGGGTGAAATGGTCCGTGTTAATGAAGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAg                                           >  2:4180631/1‑93 (MQ=255)

                                                 cGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgtgt                                        >  1:1841001/1‑93 (MQ=255)

                                                 cGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAgtgc                                        >  1:4119542/1‑92 (MQ=255)

                                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTc                                       <  2:144083/93‑1 (MQ=255)

                                                   ggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCt                                      <  2:1414832/93‑1 (MQ=255)

                                                      gAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGtt                                   >  2:2014339/1‑93 (MQ=255)

                                                          tGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTa                               >  2:4037022/1‑93 (MQ=255)

                                                          tGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTa                               >  2:5038883/1‑93 (MQ=255)

                                                          tGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTa                               <  1:3686558/93‑1 (MQ=255)

                                                           ggTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAt                              <  1:976807/93‑1 (MQ=255)

                                                            gTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATc                             <  1:575781/93‑1 (MQ=255)

                                                               cGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTc                          <  1:5327995/93‑1 (MQ=255)

                                                               cGTGTTAATGATGGTCCCTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTc                          <  2:4704062/93‑1 (MQ=255)

                                                                 tgtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCgg                        <  2:1821271/93‑1 (MQ=255)

                                                                  gtTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGt                       >  1:3836812/1‑93 (MQ=255)

                                                                   ttAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTc                      >  1:4609043/1‑93 (MQ=255)

                                                                   ttAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTc                      <  2:4440473/93‑1 (MQ=255)

                                                                    tAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCg                     >  2:3253081/1‑93 (MQ=255)

                                                                     aaTGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGCAGAGGATTACGAGAAATCTCGTCTGAAAGGGCCTGTTTCTATCTTCGGTCGt                    >  2:4491509/1‑93 (MQ=255)

                                                                     aaTGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGt                    <  1:4640149/93‑1 (MQ=255)

                                                                      atgatgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTg                   <  2:6509800/93‑1 (MQ=255)

                                                                          tgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAc               >  1:2243035/1‑93 (MQ=255)

                                                                          tgGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAc               >  1:6202020/1‑93 (MQ=255)

                                                                           ggTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcc              <  1:2396875/93‑1 (MQ=255)

                                                                            gTCCGTTCGCTGACGTCAACGTTGTTGTTGAAGAAGTGGATTACGACAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAccc             <  1:114919/93‑1 (MQ=255)

                                                                            gTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAccc             <  2:6288061/93‑1 (MQ=255)

                                                                             tCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAcccc            <  1:766322/93‑1 (MQ=255)

                                                                                gTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGt         >  2:5258177/1‑93 (MQ=255)

                                                                                 ttCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTa        >  1:3149611/1‑93 (MQ=255)

                                                                                  tCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag       <  2:781447/93‑1 (MQ=255)

                                                                                  tCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag       >  1:1420950/1‑93 (MQ=255)

                                                                                   cGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTaga      >  2:6162246/1‑93 (MQ=255)

                                                                                    gCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTagag     <  1:5074770/93‑1 (MQ=255)

                                                                                    gCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTagag     >  2:6011372/1‑93 (MQ=255)

                                                                                    gCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTagag     >  1:4002488/1‑93 (MQ=255)

                                                                                    gCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTagag     >  1:6594135/1‑93 (MQ=255)

                                                                                    gCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTagag     >  1:669001/1‑93 (MQ=255)

                                                                                    gCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTagag     >  2:2987487/1‑93 (MQ=255)

                                                                                     cTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc    <  2:5859321/93‑1 (MQ=255)

                                                                                     cTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc    >  2:1211651/1‑93 (MQ=255)

                                                                                     cTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGc    <  2:3958377/93‑1 (MQ=255)

                                                                                      tGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCt   >  1:5180667/1‑93 (MQ=255)

                                                                                      tGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCt   >  1:5245196/1‑93 (MQ=255)

                                                                                      tGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCt   >  1:6236011/1‑93 (MQ=255)

                                                                                      tGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCt   >  2:2736345/1‑93 (MQ=255)

                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg  >  2:3440053/1‑93 (MQ=255)

                                                                                       gACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGGCGTGCGACCCCGGTAGAGCTg  >  1:4289763/1‑93 (MQ=255)

CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG  >  NC_000913/4175661‑4175840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  NC_000913/4175658‑4175844

AACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACG                                                                                               >  SRR4302547.6751157/1‑94 (MQ=60)

AACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACG                                                                                               >  SRR4302547.3557035/1‑94 (MQ=60)

 cctgctTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGT                                                                                              >  SRR4302547.5284890/7‑94 (MQ=60)

 ACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGT                                                                                              >  SRR4302547.740808/1‑94 (MQ=60)

  CCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTC                                                                                             >  SRR4302547.4311536/1‑94 (MQ=60)

  CCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTC                                                                                             >  SRR4302547.4143008/1‑94 (MQ=60)

   CGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCA                                                                                            >  SRR4302547.3325291/1‑94 (MQ=60)

    GCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAA                                                                                           <  SRR4302547.246200/94‑1 (MQ=60)

    GCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAA                                                                                           <  SRR4302547.2465853/94‑1 (MQ=60)

     CCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAC                                                                                          <  SRR4302547.2028134/94‑1 (MQ=60)

     CCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAAC                                                                                          <  SRR4302547.438908/94‑1 (MQ=60)

      CTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACG                                                                                         <  SRR4302547.6246008/94‑1 (MQ=60)

       TGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGG                                                                                        >  SRR4302547.573345/1‑94 (MQ=60)

        GCAGCAGGTCGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGATGTCAACGGT                                                                                       <  SRR4302547.6608828/94‑1 (MQ=60)

          AGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGT                                                                                     >  SRR4302547.1376952/1‑94 (MQ=60)

           GCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTT                                                                                    <  SRR4302547.738681/94‑1 (MQ=60)

            CAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTG                                                                                   >  SRR4302547.5049296/1‑94 (MQ=60)

            CAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTG                                                                                   <  SRR4302547.897121/94‑1 (MQ=60)

             AGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGT                                                                                  >  SRR4302547.4821138/1‑94 (MQ=60)

             AGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGT                                                                                  >  SRR4302547.1777012/1‑94 (MQ=60)

              GGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTT                                                                                 <  SRR4302547.5808841/94‑1 (MQ=60)

               GTTGGTGATAAGCCGCGTCCGAAACCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTG                                                                                <  SRR4302547.4168060/94‑1 (MQ=60)

               GTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAGATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTG                                                                                >  SRR4302547.3276574/1‑94 (MQ=60)

               GTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTG                                                                                >  SRR4302547.5482417/1‑94 (MQ=60)

                TTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGA                                                                               >  SRR4302547.682692/1‑94 (MQ=60)

                TTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGA                                                                               >  SRR4302547.5805115/1‑94 (MQ=60)

                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              <  SRR4302547.6261919/94‑1 (MQ=60)

                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              <  SRR4302547.3366010/94‑1 (MQ=60)

                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              <  SRR4302547.6235912/94‑1 (MQ=60)

                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              <  SRR4302547.655967/94‑1 (MQ=60)

                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              >  SRR4302547.50895/1‑94 (MQ=60)

                 TGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAA                                                                              <  SRR4302547.5792851/94‑1 (MQ=60)

                  GGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAG                                                                             >  SRR4302547.2870840/1‑94 (MQ=60)

                   GTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGA                                                                            >  SRR4302547.2472878/1‑94 (MQ=60)

                    TGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAA                                                                           >  SRR4302547.5980470/1‑94 (MQ=60)

                    TGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAA                                                                           <  SRR4302547.4499097/94‑1 (MQ=60)

                    TGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAA                                                                           >  SRR4302547.782199/1‑94 (MQ=60)

                     GATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          >  SRR4302547.5857908/1‑94 (MQ=60)

                     GATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          <  SRR4302547.3043048/94‑1 (MQ=60)

                     GATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          <  SRR4302547.3370307/94‑1 (MQ=60)

                     GATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAG                                                                          >  SRR4302547.2328633/1‑94 (MQ=60)

                      ATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGT                                                                         <  SRR4302547.1181501/94‑1 (MQ=60)

                      ATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGT                                                                         <  SRR4302547.134593/94‑1 (MQ=60)

                       TAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTG                                                                        <  SRR4302547.6353362/94‑1 (MQ=60)

                       TAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTG                                                                        <  SRR4302547.1907834/94‑1 (MQ=60)

                       TAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTG                                                                        <  SRR4302547.514267/94‑1 (MQ=60)

                        AAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGG                                                                       <  SRR4302547.213346/94‑1 (MQ=60)

                        AAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGG                                                                       <  SRR4302547.4077558/94‑1 (MQ=60)

                        AAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGG                                                                       <  SRR4302547.287480/94‑1 (MQ=60)

                        AAGCCGCGTCCGAAAACGCTGTATGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGG                                                                       >  SRR4302547.4314445/1‑94 (MQ=60)

                         AGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGA                                                                      <  SRR4302547.3778123/94‑1 (MQ=60)

                          GCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGAT                                                                     <  SRR4302547.842983/94‑1 (MQ=60)

                             GCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAC                                                                  <  SRR4302547.1422499/94‑1 (MQ=60)

                             GCGTCAGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTAC                                                                  <  SRR4302547.6378751/94‑1 (MQ=60)

                                TCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACAAG                                                               <  SRR4302547.6491493/94‑1 (MQ=60)

                                  CGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAA                                                             >  SRR4302547.725738/1‑94 (MQ=60)

                                  CGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAA                                                             >  SRR4302547.4796675/1‑94 (MQ=60)

                                  CGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAA                                                             >  SRR4302547.3523063/1‑94 (MQ=60)

                                  CGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAA                                                             <  SRR4302547.595263/94‑1 (MQ=60)

                                  CGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAA                                                             <  SRR4302547.4725430/94‑1 (MQ=60)

                                   GAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAA                                                            >  SRR4302547.1427239/1‑94 (MQ=60)

                                      AACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCT                                                         >  SRR4302547.2356651/1‑94 (MQ=60)

                                      AACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCT                                                         >  SRR4302547.5995949/1‑94 (MQ=60)

                                       ACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTC                                                        >  SRR4302547.6417937/1‑94 (MQ=60)

                                        CGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCG                                                       <  SRR4302547.5724171/94‑1 (MQ=60)

                                        CGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCG                                                       >  SRR4302547.4711810/1‑94 (MQ=60)

                                         GCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCNACNGTNTTGTTGAAGAAGTGGATTACGAGAAATCTCGT                                                      >  SRR4302547.5372457/1‑94 (MQ=60)

                                          CTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTC                                                     >  SRR4302547.2952729/1‑94 (MQ=60)

                                          CTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTC                                                     >  SRR4302547.845607/1‑94 (MQ=60)

                                           TGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCT                                                    >  SRR4302547.3761691/1‑94 (MQ=60)

                                            GTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTG                                                   <  SRR4302547.4168072/94‑1 (MQ=60)

                                            GTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTG                                                   >  SRR4302547.5811973/1‑94 (MQ=60)

                                             TTGGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGA                                                  <  SRR4302547.1096837/94‑1 (MQ=60)

                                              TTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAA                                                 <  SRR4302547.1419540/94‑1 (MQ=60)

                                                GAGCCGGGTGAAATGGTCAGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAG                                               <  SRR4302547.2655303/94‑1 (MQ=60)

                                                GAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAG                                               >  SRR4302547.6166113/1‑94 (MQ=60)

                                                GAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAG                                               <  SRR4302547.1303391/94‑1 (MQ=60)

                                                GAACCGGGTGAAATGGTCCGTGGTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAG                                               <  SRR4302547.806995/94‑1 (MQ=60)

                                                   CCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGATAGTGC                                            >  SRR4302547.3979875/1‑94 (MQ=60)

                                                   CCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGT                                            >  SRR4302547.6180544/1‑94 (MQ=60)

                                                   CCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGT                                            >  SRR4302547.1877383/1‑94 (MQ=60)

                                                   CCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGC                                            >  SRR4302547.4203265/1‑94 (MQ=60)

                                                     GGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCT                                          <  SRR4302547.5288366/94‑1 (MQ=60)

                                                     GGGTGAAAGGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCT                                          >  SRR4302547.5481377/1‑94 (MQ=60)

                                                      GGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTG                                         <  SRR4302547.6310467/94‑1 (MQ=60)

                                                      GGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTG                                         >  SRR4302547.1965868/1‑94 (MQ=60)

                                                      GGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTG                                         <  SRR4302547.700935/94‑1 (MQ=60)

                                                       GTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGT                                        <  SRR4302547.2188678/94‑1 (MQ=60)

                                                       GTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGT                                        <  SRR4302547.6250167/94‑1 (MQ=60)

                                                        TGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTT                                       <  SRR4302547.1384255/94‑1 (MQ=60)

                                                        TGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTT                                       >  SRR4302547.532966/1‑94 (MQ=60)

                                                        TGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTT                                       <  SRR4302547.2331473/94‑1 (MQ=60)

                                                        TGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTT                                       <  SRR4302547.2535706/94‑1 (MQ=60)

                                                         GAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTT                                      <  SRR4302547.3875137/94‑1 (MQ=60)

                                                          AAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTC                                     >  SRR4302547.5235175/1‑94 (MQ=60)

                                                           AATGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCT                                    >  SRR4302547.608685/1‑94 (MQ=60)

                                                             TGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAT                                  <  SRR4302547.3761822/94‑1 (MQ=60)

                                                             TGGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTAT                                  <  SRR4302547.4121826/94‑1 (MQ=60)

                                                              GGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATC                                 <  SRR4302547.995803/94‑1 (MQ=60)

                                                              GGTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATC                                 >  SRR4302547.353007/1‑94 (MQ=60)

                                                               GTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCT                                <  SRR4302547.2630687/94‑1 (MQ=60)

                                                               GTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCT                                <  SRR4302547.586105/94‑1 (MQ=60)

                                                               GTCCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCT                                <  SRR4302547.2067781/94‑1 (MQ=60)

                                                                 CCGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTC                              >  SRR4302547.4953876/1‑94 (MQ=60)

                                                                  CGTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             <  SRR4302547.5437815/94‑1 (MQ=60)

                                                                  CGTGTGAATGATGGTCCGGTCGCGGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCG                             <  SRR4302547.1292632/94‑1 (MQ=60)

                                                                   GTGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGG                            <  SRR4302547.4691806/94‑1 (MQ=60)

                                                                    TGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGT                           >  SRR4302547.992656/1‑94 (MQ=60)

                                                                    TGTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGT                           >  SRR4302547.3914961/1‑94 (MQ=60)

                                                                     GTTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTC                          >  SRR4302547.4704436/1‑94 (MQ=60)

                                                                      TTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCG                         <  SRR4302547.6764874/94‑1 (MQ=60)

                                                                      TTAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCG                         <  SRR4302547.6717873/94‑1 (MQ=60)

                                                                       TAATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGT                        <  SRR4302547.5832802/94‑1 (MQ=60)

                                                                        AATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTG                       <  SRR4302547.4736253/94‑1 (MQ=60)

                                                                         ATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGC                      >  SRR4302547.271244/1‑94 (MQ=60)

                                                                         ATGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGC                      >  SRR4302547.1607441/1‑94 (MQ=60)

                                                                          TGATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCG                     <  SRR4302547.4755850/94‑1 (MQ=60)

                                                                            ATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAC                   >  SRR4302547.6330789/1‑94 (MQ=60)

                                                                            ATGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAC                   >  SRR4302547.2288273/1‑94 (MQ=60)

                                                                             TGGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACC                  <  SRR4302547.4480702/94‑1 (MQ=60)

                                                                              GGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAGCC                 >  SRR4302547.6570045/1‑94 (MQ=60)

                                                                              GGTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCC                 <  SRR4302547.2445432/94‑1 (MQ=60)

                                                                               GTCCGTTCGCTGACGTCAACGTTGTTGTTGAAGAAGTGGATTACGACAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCC                <  SRR4302547.117009/94‑1 (MQ=60)

                                                                               GTCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCC                >  SRR4302547.2795452/1‑94 (MQ=60)

                                                                                TCCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCG               <  SRR4302547.780789/94‑1 (MQ=60)

                                                                                 CCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGG              <  SRR4302547.980506/94‑1 (MQ=60)

                                                                                 CCGTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGG              >  SRR4302547.5212837/1‑94 (MQ=60)

                                                                                   GTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTA            <  SRR4302547.216966/94‑1 (MQ=60)

                                                                                   GTTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTA            >  SRR4302547.3212205/1‑94 (MQ=60)

                                                                                    TTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGCCCCCGGTAG           <  SRR4302547.6618657/94‑1 (MQ=60)

                                                                                    TTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAG           >  SRR4302547.3115957/1‑94 (MQ=60)

                                                                                    TTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAG           >  SRR4302547.1449391/1‑94 (MQ=60)

                                                                                    TTCGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAG           <  SRR4302547.6313631/94‑1 (MQ=60)

                                                                                      CGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAG         >  SRR4302547.6731424/1‑94 (MQ=60)

                                                                                      CGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAG         >  SRR4302547.681313/1‑94 (MQ=60)

                                                                                      CGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAG         >  SRR4302547.4083848/1‑94 (MQ=60)

                                                                                      CGCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAG         <  SRR4302547.1102332/94‑1 (MQ=60)

                                                                                       GCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGC        <  SRR4302547.5179465/94‑1 (MQ=60)

                                                                                       GCTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGC        <  SRR4302547.726748/94‑1 (MQ=60)

                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       >  SRR4302547.5353155/1‑94 (MQ=60)

                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       >  SRR4302547.6365567/1‑94 (MQ=60)

                                                                                        CTGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCT       >  SRR4302547.5287291/1‑94 (MQ=60)

                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG      >  SRR4302547.1303863/1‑94 (MQ=60)

                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTG      >  SRR4302547.6126714/1‑94 (MQ=60)

                                                                                         TGACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGGCGTGCGACCCCGGTAGAGCTG      >  SRR4302547.4377931/1‑94 (MQ=60)

                                                                                          GACGTCAGCGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGG     <  SRR4302547.2425686/94‑1 (MQ=60)

                                                                                          GACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGG     >  SRR4302547.1426101/1‑94 (MQ=60)

                                                                                           ACGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGA    <  SRR4302547.1940048/94‑1 (MQ=60)

                                                                                            CGTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGAC   >  SRR4302547.2860929/1‑94 (MQ=60)

                                                                                             GTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  SRR4302547.4914031/1‑94 (MQ=60)

                                                                                             GTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  SRR4302547.1017713/1‑94 (MQ=60)

AACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACT  >  NC_000913/4175658‑4175844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`