Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,181,376 G→A A852T (GCT→ACT)  rpoB → RNA polymerase beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,3760GA85.6% 784.9 / 92.1 278A852T (GCT→ACT) rpoBRNA polymerase beta subunit
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base A (106/132);  total (124/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-01

Only 100 of 292 total aligned reads displayed.

GTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATT  >  NC_000913/4181284‑4181468
                                                                                            |                                                                                            
   ttgttCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTg                                                                                           >  1:1112921/1‑93 (MQ=255)
     gttCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGAc                                                                                         <  1:1239680/93‑1 (MQ=255)
        cAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATc                                                                                      <  1:586986/93‑1 (MQ=255)
        cAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATc                                                                                      >  2:6257224/1‑93 (MQ=255)
         aGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGTCACCAAGCTGGGTTCGGAAGAGATCACGCCTGACATcc                                                                                     >  1:5380300/1‑93 (MQ=255)
         aGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATcc                                                                                     >  2:6563833/1‑93 (MQ=255)
         aGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATcc                                                                                     <  2:6113599/93‑1 (MQ=255)
         aGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATcc                                                                                     >  2:3455251/1‑93 (MQ=255)
            aaGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGa                                                                                  <  2:4835943/93‑1 (MQ=255)
             acaCCGTTTCACAACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGaa                                                                                 <  2:646345/91‑1 (MQ=255)
              gACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACGAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAAc                                                                                <  2:4965955/93‑1 (MQ=255)
              gACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAAc                                                                                >  1:5918081/1‑93 (MQ=255)
              aaCCGTTACACCACCATCCACATTCAGGAACGGGCGTTTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAAc                                                                                <  1:4463320/92‑1 (MQ=255)
               aCCTTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACg                                                                               <  2:4736307/93‑1 (MQ=255)
               aCCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACg                                                                               <  1:922488/93‑1 (MQ=255)
                ccGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGt                                                                              >  1:2819831/1‑93 (MQ=255)
                 cGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTg                                                                             >  2:4638404/1‑93 (MQ=255)
                 cGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTg                                                                             <  2:4054385/93‑1 (MQ=255)
                  gTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTgg                                                                            >  2:3869753/1‑93 (MQ=255)
                   tttCACCACCATCCACATTCAGGAACTGGCGTGTGTGTTCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTggg                                                                           >  1:2040930/1‑93 (MQ=255)
                   tttCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTggg                                                                           <  1:5080702/93‑1 (MQ=255)
                    ttCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGt                                                                          <  2:6078485/93‑1 (MQ=255)
                     tCACCCCCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTg                                                                         <  1:1654325/93‑1 (MQ=255)
                     tCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTg                                                                         <  2:2958622/93‑1 (MQ=255)
                     tCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTg                                                                         <  2:1947089/93‑1 (MQ=255)
                       accaccATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGaa                                                                       >  2:2591442/1‑93 (MQ=255)
                       accaccATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGaa                                                                       <  2:4236514/93‑1 (MQ=255)
                       accaccATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGACCGGAATAGATGACCACTGACATCCCGAACGTGGGTgga                                                                       >  1:2764461/1‑91 (MQ=255)
                        ccaccaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAg                                                                      >  2:1234635/1‑93 (MQ=255)
                        ccaccaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAg                                                                      >  2:517391/1‑93 (MQ=255)
                        ccaccaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAg                                                                      <  2:5109881/93‑1 (MQ=255)
                        ccaccaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAg                                                                      <  2:805800/93‑1 (MQ=255)
                         caccaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGc                                                                     <  1:3171156/93‑1 (MQ=255)
                          cccaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCt                                                                    <  1:2355525/92‑1 (MQ=255)
                          accaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCt                                                                    <  1:716772/93‑1 (MQ=255)
                           ccaTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTg                                                                   >  1:2702931/1‑93 (MQ=255)
                            caTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGTAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTgc                                                                  <  2:4792916/93‑1 (MQ=255)
                            caTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCCCTGACATCCCGCACGTGGGTGACGCTgg                                                                  >  1:552756/1‑92 (MQ=255)
                            caTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTgc                                                                  <  1:5738434/93‑1 (MQ=255)
                               ccACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGct                                                               <  1:3368952/93‑1 (MQ=255)
                                 acaTTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGctct                                                             <  2:6348097/93‑1 (MQ=255)
                                  caTTCAGGAACTGGAGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGCAGATATCACCACTGAGATAGCGAACGTGGGGGGAGCCGCGCgctc                                                            >  1:2957954/1‑93 (MQ=255)
                                   aTTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTcc                                                           <  2:18233/93‑1 (MQ=255)
                                   aTTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTcc                                                           >  1:3743861/1‑93 (MQ=255)
                                    ttCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCa                                                          <  1:2201405/93‑1 (MQ=255)
                                    ttCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCa                                                          >  2:3628327/1‑93 (MQ=255)
                                    ttCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCa                                                          >  2:2894333/1‑93 (MQ=255)
                                     tCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCaa                                                         >  1:3600321/1‑93 (MQ=255)
                                      cAGGAACTGGCGTGTGTGTTCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCaaa                                                        >  1:6496045/1‑93 (MQ=255)
                                      cAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCaaa                                                        >  2:3754238/1‑93 (MQ=255)
                                       aGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAAc                                                       <  2:5785948/93‑1 (MQ=255)
                                       aGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAAc                                                       >  1:4804491/1‑93 (MQ=255)
                                           aCTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGa                                                   <  2:1686438/93‑1 (MQ=255)
                                           aCTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGa                                                   <  2:1780862/93‑1 (MQ=255)
                                            cTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAt                                                  <  2:2445394/93‑1 (MQ=255)
                                            cTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAt                                                  <  2:5758441/93‑1 (MQ=255)
                                            cTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAt                                                  >  2:5353822/1‑93 (MQ=255)
                                            cTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAt                                                  <  2:4147844/93‑1 (MQ=255)
                                               gCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGaa                                               >  1:3234385/1‑93 (MQ=255)
                                                cGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAAt                                              >  1:4917420/1‑93 (MQ=255)
                                                 gtgtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATc                                             <  1:1771994/93‑1 (MQ=255)
                                                  tgtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATcc                                            <  2:5275258/93‑1 (MQ=255)
                                                  tgtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATcc                                            >  1:408736/1‑93 (MQ=255)
                                                  tgtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATcc                                            <  1:814472/93‑1 (MQ=255)
                                                  tgtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATcc                                            >  1:3770512/1‑93 (MQ=255)
                                                     gtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGt                                         >  2:1060062/1‑93 (MQ=255)
                                                     gtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGt                                         >  1:5283285/1‑93 (MQ=255)
                                                      tgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTa                                        <  1:2622644/93‑1 (MQ=255)
                                                        tCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATc                                      >  2:1035079/1‑93 (MQ=255)
                                                          ccGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGt                                    <  1:4702895/93‑1 (MQ=255)
                                                               acacCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACa                               <  2:4494650/93‑1 (MQ=255)
                                                               acacCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACa                               >  2:3678397/1‑93 (MQ=255)
                                                                cacCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACAt                              >  1:3102264/1‑93 (MQ=255)
                                                                   cAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTgg                           <  1:5977281/93‑1 (MQ=255)
                                                                    aaGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGt                          >  1:6394005/1‑93 (MQ=255)
                                                                    aaGCGGGATCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGt                          <  1:1377803/93‑1 (MQ=255)
                                                                     aGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTg                         <  2:2288089/93‑1 (MQ=255)
                                                                     aGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTg                         <  1:2384858/93‑1 (MQ=255)
                                                                       cTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCg                       <  2:4166173/93‑1 (MQ=255)
                                                                        tGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCgg                      >  1:5211925/1‑93 (MQ=255)
                                                                        tGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCgg                      <  1:5609407/93‑1 (MQ=255)
                                                                          ggTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGaa                    >  1:1483706/1‑93 (MQ=255)
                                                                          ggTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGaa                    <  1:4239658/93‑1 (MQ=255)
                                                                           gTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAg                   <  1:4310740/93‑1 (MQ=255)
                                                                              cGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGa                >  2:6449645/1‑93 (MQ=255)
                                                                              cGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAACCTGGATGAATCCGGTATCGTTTACATTGGTGCGGACGTGa                >  2:1718390/1‑93 (MQ=255)
                                                                               ggAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAc               >  2:1435844/1‑93 (MQ=255)
                                                                               ggAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAc               <  1:4103036/93‑1 (MQ=255)
                                                                                gAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAcc              <  2:3098645/93‑1 (MQ=255)
                                                                                 aaGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCg             <  1:4868988/93‑1 (MQ=255)
                                                                                  agagATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCgg            >  2:5548992/1‑93 (MQ=255)
                                                                                   gagaTCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCggg           >  2:2162847/1‑92 (MQ=255)
                                                                                   gagaTCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGt           <  1:5498237/93‑1 (MQ=255)
                                                                                   gagaTCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGt           <  2:1512456/93‑1 (MQ=255)
                                                                                       tCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCg       <  1:6132538/93‑1 (MQ=255)
                                                                                       tCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCg       <  2:160920/93‑1 (MQ=255)
                                                                                        caccacTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGa      <  2:2260089/93‑1 (MQ=255)
                                                                                        cACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGa      >  1:6065873/1‑93 (MQ=255)
                                                                                         aCCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGAc     <  1:3914632/93‑1 (MQ=255)
                                                                                            gCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAtt  <  2:4005903/93‑1 (MQ=255)
                                                                                            |                                                                                            
GTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATT  >  NC_000913/4181284‑4181468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATT  >  NC_000913/4181283‑4181468
                                                                                             |                                                                                            
CGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCC                                                                                              >  SRR4302547.5112548/1‑94 (MQ=60)
CGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCA                                                                                              <  SRR4302547.4200405/94‑1 (MQ=60)
 GTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCAC                                                                                             >  SRR4302547.1590783/1‑94 (MQ=60)
 GTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCAC                                                                                             <  SRR4302547.5320170/94‑1 (MQ=60)
 GTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCCGAAGAGACCACCAC                                                                                             >  SRR4302547.576816/1‑94 (MQ=60)
  TGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCT                                                                                            <  SRR4302547.5120432/94‑1 (MQ=60)
  TGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACT                                                                                            >  SRR4302547.2344439/1‑94 (MQ=60)
   GTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTG                                                                                           >  SRR4302547.1135333/1‑94 (MQ=60)
    TTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGA                                                                                          <  SRR4302547.4511203/94‑1 (MQ=60)
      GTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACA                                                                                        <  SRR4302547.1264716/94‑1 (MQ=60)
      GTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACA                                                                                        <  SRR4302547.5883044/94‑1 (MQ=60)
       TTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACAT                                                                                       <  SRR4302547.5076296/94‑1 (MQ=60)
         CAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGTCACCAAGCTGGGTTCGGAAGAGATCACGCCTGACATCC                                                                                     >  SRR4302547.5491383/1‑94 (MQ=60)
         CAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCC                                                                                     <  SRR4302547.597575/94‑1 (MQ=60)
         CAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCC                                                                                     >  SRR4302547.3554910/1‑94 (MQ=60)
         CAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCC                                                                                     <  SRR4302547.2413284/94‑1 (MQ=60)
         CAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCAGGGTCCGGAAGAGATCACCACTGACATCC                                                                                     >  SRR4302547.6668710/1‑94 (MQ=60)
          AGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCC                                                                                    <  SRR4302547.3187027/94‑1 (MQ=60)
          AAGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCC                                                                                    >  SRR4302547.1392502/1‑94 (MQ=60)
           GGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCG                                                                                   >  SRR4302547.1918740/1‑94 (MQ=60)
           GGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCG                                                                                   <  SRR4302547.3670291/94‑1 (MQ=60)
             AAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAA                                                                                 >  SRR4302547.1725353/1‑94 (MQ=60)
             AAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAA                                                                                 >  SRR4302547.6310128/1‑94 (MQ=60)
             AAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAA                                                                                 >  SRR4302547.758714/1‑94 (MQ=60)
              AGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAAC                                                                                <  SRR4302547.5243417/94‑1 (MQ=60)
              AGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAAC                                                                                >  SRR4302547.5437272/1‑94 (MQ=60)
              AGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAAC                                                                                >  SRR4302547.6040729/1‑94 (MQ=60)
              AGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAAC                                                                                >  SRR4302547.1699098/1‑94 (MQ=60)
               AACCGTTACACCACCATCCACATTCAGGAACGGGCGTTTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACG                                                                               <  SRR4302547.4555556/94‑1 (MQ=60)
                ACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGT                                                                              >  SRR4302547.2876240/1‑94 (MQ=60)
                ACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGT                                                                              <  SRR4302547.940308/94‑1 (MQ=60)
                 CCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTG                                                                             >  SRR4302547.1474769/1‑94 (MQ=60)
                 CCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTG                                                                             <  SRR4302547.5005080/94‑1 (MQ=60)
                 CCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTG                                                                             <  SRR4302547.2878243/94‑1 (MQ=60)
                 CCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTG                                                                             <  SRR4302547.2824055/94‑1 (MQ=60)
                  CGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGG                                                                            <  SRR4302547.4356694/94‑1 (MQ=60)
                  CGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGG                                                                            <  SRR4302547.6249551/94‑1 (MQ=60)
                   GTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTTCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGG                                                                           >  SRR4302547.2081633/1‑94 (MQ=60)
                   GTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGG                                                                           <  SRR4302547.2116742/94‑1 (MQ=60)
                    TTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGT                                                                          <  SRR4302547.5185536/94‑1 (MQ=60)
                      TCACCCCCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGA                                                                        <  SRR4302547.1686965/94‑1 (MQ=60)
                      TCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGA                                                                        <  SRR4302547.6546465/94‑1 (MQ=60)
                      TCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGA                                                                        <  SRR4302547.2718862/94‑1 (MQ=60)
                       CACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAA                                                                       >  SRR4302547.4979840/1‑94 (MQ=60)
                       CACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGACCGGAATAGATGACCACTGACATCCCGAACGTGGGTGGA                                                                       >  SRR4302547.2819861/1‑94 (MQ=60)
                         CCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGC                                                                     >  SRR4302547.4920511/1‑94 (MQ=60)
                          CACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCT                                                                    <  SRR4302547.3137716/94‑1 (MQ=60)
                          CACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCT                                                                    <  SRR4302547.6341140/94‑1 (MQ=60)
                          CACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCT                                                                    <  SRR4302547.3234269/94‑1 (MQ=60)
                           CCCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTG                                                                   <  SRR4302547.2403259/94‑1 (MQ=60)
                           ACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTG                                                                   >  SRR4302547.2765867/1‑94 (MQ=60)
                           ACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTG                                                                   <  SRR4302547.730052/94‑1 (MQ=60)
                           ACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTG                                                                   >  SRR4302547.2757175/1‑94 (MQ=60)
                            CCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCCCTGACATCCCGCACGTGGGTGACGCTGG                                                                  >  SRR4302547.562716/1‑94 (MQ=60)
                             CATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCCAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGAGAAGCTGCG                                                                 >  SRR4302547.2695439/1‑94 (MQ=60)
                             CATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCG                                                                 <  SRR4302547.5857018/94‑1 (MQ=60)
                              ATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGC                                                                >  SRR4302547.3957109/1‑94 (MQ=60)
                               TCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCT                                                               <  SRR4302547.916003/94‑1 (MQ=60)
                                CCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTC                                                              <  SRR4302547.3437378/94‑1 (MQ=60)
                                CCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTC                                                              >  SRR4302547.5384020/1‑94 (MQ=60)
                                 CACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCT                                                             >  SRR4302547.1368217/1‑94 (MQ=60)
                                   CATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCC                                                           <  SRR4302547.4170728/94‑1 (MQ=60)
                                   CATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCC                                                           <  SRR4302547.3833213/94‑1 (MQ=60)
                                   CATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCC                                                           >  SRR4302547.3820283/1‑94 (MQ=60)
                                    ATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCA                                                          >  SRR4302547.6599683/1‑94 (MQ=60)
                                    ATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCA                                                          <  SRR4302547.5509921/94‑1 (MQ=60)
                                     TTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAA                                                         <  SRR4302547.2245632/94‑1 (MQ=60)
                                     TTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAA                                                         >  SRR4302547.3673736/1‑94 (MQ=60)
                                      TCAGGAACTGGCGTGTGTGTTCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAA                                                        >  SRR4302547.6631266/1‑94 (MQ=60)
                                      TCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAA                                                        <  SRR4302547.5683262/94‑1 (MQ=60)
                                       CAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAAC                                                       >  SRR4302547.5761883/1‑94 (MQ=60)
                                       CAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAAC                                                       >  SRR4302547.4904056/1‑94 (MQ=60)
                                       CAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAAC                                                       >  SRR4302547.3278854/1‑94 (MQ=60)
                                        AGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACT                                                      <  SRR4302547.4835836/94‑1 (MQ=60)
                                        AGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACT                                                      >  SRR4302547.714570/1‑94 (MQ=60)
                                        AGGAACTGGCGTGTGTGTCCAGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGCAGCTGCGCTCTCCAAACT                                                      >  SRR4302547.5390776/1‑94 (MQ=60)
                                         GGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTG                                                     >  SRR4302547.5629283/1‑94 (MQ=60)
                                         GGAACTGCCGGGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACACCCCGAACGT‑GGTGAAGCTGCGCTCTCCAAACTGG                                                    <  SRR4302547.2814720/94‑1 (MQ=60)
                                            ACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAT                                                  >  SRR4302547.6024367/1‑94 (MQ=60)
                                            ACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAT                                                  <  SRR4302547.1000712/94‑1 (MQ=60)
                                             CTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATG                                                 <  SRR4302547.607013/94‑1 (MQ=60)
                                             CTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATG                                                 <  SRR4302547.2378328/94‑1 (MQ=60)
                                               GGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAA                                               >  SRR4302547.3298938/1‑94 (MQ=60)
                                               GGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAA                                               >  SRR4302547.3979985/1‑94 (MQ=60)
                                                GCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAAT                                              <  SRR4302547.1837009/94‑1 (MQ=60)
                                                GCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAAT                                              >  SRR4302547.5019263/1‑94 (MQ=60)
                                                 CGTGTGTGTCCCGTGACACAAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGACGCTGCGCTCTCCAAACTGGATGgagc                                             >  SRR4302547.1683113/1‑90 (MQ=60)
                                                  GTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCC                                            <  SRR4302547.1807126/94‑1 (MQ=60)
                                                  GTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCC                                            >  SRR4302547.3847399/1‑94 (MQ=60)
                                                  GTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCC                                            >  SRR4302547.416319/1‑94 (MQ=60)
                                                   TGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCG                                           <  SRR4302547.5571987/94‑1 (MQ=60)
                                                   TGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCG                                           <  SRR4302547.829937/94‑1 (MQ=60)
                                                     ggtgacgcgtgcatccacgcgGGGTCCAGAAGAGATCTCCACTGACATCCCGAACGTGGGTGAAGCTGCGCgatcgatacgggatgaaaccgct                                         <  SRR4302547.6462624/73‑24 (MQ=44)
                                                     TGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGT                                         >  SRR4302547.5392271/1‑94 (MQ=60)
                                                     TGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATACGGT                                         >  SRR4302547.2568235/1‑94 (MQ=60)
                                                      GTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTA                                        <  SRR4302547.5253927/94‑1 (MQ=60)
                                                       TGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTAT                                       <  SRR4302547.5443940/94‑1 (MQ=60)
                                                       TGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTAT                                       <  SRR4302547.2675472/94‑1 (MQ=60)
                                                          CCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGT                                    >  SRR4302547.3810369/1‑94 (MQ=60)
                                                           CCGTGACACCAAGCTGGGTCCGGAAGAGATCACGGCTGACATCCCGAACGTGGGTGAAGCTGAGCTCTGCAAACTGGATGAATCCGGTATCGAT                                   >  SRR4302547.3292717/1‑94 (MQ=60)
                                                           CCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTT                                   <  SRR4302547.399706/94‑1 (MQ=60)
                                                           CCGTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTT                                   <  SRR4302547.4800292/94‑1 (MQ=60)
                                                            CGTGTCACCAAGATGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTATCCAAACTGGATGAATCCGGTATCGTTT                                  <  SRR4302547.489958/94‑1 (MQ=60)
                                                             GTGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTA                                 >  SRR4302547.4177448/1‑94 (MQ=60)
                                                              TGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTAC                                <  SRR4302547.5630909/94‑1 (MQ=60)
                                                              TGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTAC                                <  SRR4302547.4508194/94‑1 (MQ=60)
                                                              TGACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCG‑TTACA                               >  SRR4302547.4027203/1‑94 (MQ=60)
                                                                ACACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACAT                              >  SRR4302547.3163745/1‑94 (MQ=60)
                                                                 CACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATT                             <  SRR4302547.3320707/94‑1 (MQ=60)
                                                                 CACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATT                             >  SRR4302547.3826603/1‑94 (MQ=60)
                                                                 CACCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATT                             <  SRR4302547.1654164/94‑1 (MQ=60)
                                                                   CCAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGG                           >  SRR4302547.454948/1‑94 (MQ=60)
                                                                    CAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGT                          >  SRR4302547.6527393/1‑94 (MQ=60)
                                                                    CAAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGT                          <  SRR4302547.6101268/94‑1 (MQ=60)
                                                                     AAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAGCTGGATGAATCCGGTATCGTTTACATTGGTG                         >  SRR4302547.6078065/1‑94 (MQ=60)
                                                                     AAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTG                         <  SRR4302547.2999228/94‑1 (MQ=60)
                                                                     AAGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCAACAAACTGGATGAATCCGGTATCGTTTACATTGGTG                         <  SRR4302547.3805326/94‑1 (MQ=60)
                                                                     AAGCGGGATCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTG                         <  SRR4302547.1405425/94‑1 (MQ=60)
                                                                      AGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGC                        <  SRR4302547.914096/94‑1 (MQ=60)
                                                                      AGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGC                        <  SRR4302547.2433170/94‑1 (MQ=60)
                                                                      AGCTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGC                        <  SRR4302547.5837027/94‑1 (MQ=60)
                                                                        CTGGGTCCGGAAGAGATCACCGCTGACAACCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGG                      <  SRR4302547.4778426/94‑1 (MQ=60)
                                                                        CTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGG                      >  SRR4302547.6221638/1‑94 (MQ=60)
                                                                        CTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGG                      <  SRR4302547.2576463/94‑1 (MQ=60)
                                                                        CTGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGG                      >  SRR4302547.5319206/1‑94 (MQ=60)
                                                                         TGGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGA                     <  SRR4302547.5725154/94‑1 (MQ=60)
                                                                          GGGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAA                    >  SRR4302547.1513313/1‑94 (MQ=60)
                                                                           GGTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAG                   <  SRR4302547.4326350/94‑1 (MQ=60)
                                                                            GTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGT                  <  SRR4302547.2206881/94‑1 (MQ=60)
                                                                            GTCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGT                  <  SRR4302547.4399418/94‑1 (MQ=60)
                                                                             TCCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTG                 <  SRR4302547.420639/94‑1 (MQ=60)
                                                                              CCGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGA                >  SRR4302547.1258025/1‑94 (MQ=60)
                                                                               CGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAC               >  SRR4302547.342053/1‑94 (MQ=60)
                                                                               CGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAC               <  SRR4302547.986103/94‑1 (MQ=60)
                                                                               CGGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAC               >  SRR4302547.819692/1‑94 (MQ=60)
                                                                                GGAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACC              <  SRR4302547.4186454/94‑1 (MQ=60)
                                                                                 GAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCG             >  SRR4302547.4884824/1‑94 (MQ=60)
                                                                                 GAAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCG             <  SRR4302547.3131416/94‑1 (MQ=60)
                                                                                  AAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGG            <  SRR4302547.4969889/94‑1 (MQ=60)
                                                                                  AAGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGG            <  SRR4302547.5942674/94‑1 (MQ=60)
                                                                                   AGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGT           <  SRR4302547.4240635/94‑1 (MQ=60)
                                                                                   AGAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGT           >  SRR4302547.6156211/1‑94 (MQ=60)
                                                                                    GAGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTG          <  SRR4302547.5611760/94‑1 (MQ=60)
                                                                                     AGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGG         <  SRR4302547.2803940/94‑1 (MQ=60)
                                                                                     AGATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGG         >  SRR4302547.1462192/1‑94 (MQ=60)
                                                                                       ATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCG       >  SRR4302547.3189297/1‑94 (MQ=60)
                                                                                       ATCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCG       <  SRR4302547.2432513/94‑1 (MQ=60)
                                                                                        TCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGA      >  SRR4302547.6191690/1‑94 (MQ=60)
                                                                                        TCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGA      <  SRR4302547.6259741/94‑1 (MQ=60)
                                                                                        TCACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGA      >  SRR4302547.6178066/1‑94 (MQ=60)
                                                                                          ACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACA    <  SRR4302547.3994230/94‑1 (MQ=60)
                                                                                          ACCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACA    >  SRR4302547.2120237/1‑94 (MQ=60)
                                                                                           CCACTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAT   >  SRR4302547.5956582/1‑94 (MQ=60)
                                                                                            CGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATT  >  SRR4302547.6034905/1‑94 (MQ=60)
                                                                                             |                                                                                            
CGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATT  >  NC_000913/4181283‑4181468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑