Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,199,968	G→A	A155A (GCG→GCA)	hydH →	HydH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,199,968	0	G	A	86.3%	748.5 / 80.3	263	A155A (GCG→GCA)	hydH	HydH
Reads supporting (aligned to +/- strand): ref base G (16/20); new base A (122/105); total (138/125)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 268 total aligned reads displayed.

AGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTT  >  NC_000913/4199877‑4200058

  ccAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCAccc                                                                                         <  2:720503/93‑1 (MQ=255)

  ccAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCAccc                                                                                         <  1:650848/93‑1 (MQ=255)

  ccAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCAccc                                                                                         <  1:2372197/93‑1 (MQ=255)

   cAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCt                                                                                        <  2:5758739/93‑1 (MQ=255)

   cAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCt                                                                                        >  1:2226564/1‑93 (MQ=255)

     gagGAAAACTCCCGCTGTCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTgg                                                                                      <  2:22379/93‑1 (MQ=255)

     gagGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTgg                                                                                      >  1:5278332/1‑93 (MQ=255)

     gagGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTgg                                                                                      <  1:4492923/93‑1 (MQ=255)

     gagGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTgg                                                                                      >  1:3422038/1‑93 (MQ=255)

       ggAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTggcg                                                                                    <  1:1216929/93‑1 (MQ=255)

         aaaaCTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTggcggc                                                                                  <  2:3310850/93‑1 (MQ=255)

          aaaCTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCa                                                                                 <  2:2176901/93‑1 (MQ=255)

           aaCTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCAt                                                                                <  2:4390932/93‑1 (MQ=255)

              tCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGa                                                                             >  2:2943582/1‑93 (MQ=255)

               cccGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGaa                                                                            <  1:1718906/93‑1 (MQ=255)

                ccGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAAt                                                                           >  2:3170720/1‑93 (MQ=255)

                ccGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAAt                                                                           >  2:3855853/1‑93 (MQ=255)

                  gCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGc                                                                         <  2:2610880/93‑1 (MQ=255)

                  gCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGc                                                                         <  1:1601050/93‑1 (MQ=255)

                  gCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGc                                                                         <  2:4071933/93‑1 (MQ=255)

                    tGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGcac                                                                       >  2:2327035/1‑93 (MQ=255)

                    tGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGcac                                                                       >  1:2871234/1‑93 (MQ=255)

                        ggcTGCTTGGGAAAACGTAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCGCAata                                                                   <  1:6319904/93‑1 (MQ=255)

                        ggcTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAata                                                                   >  1:2133886/1‑93 (MQ=255)

                        ggcTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAata                                                                   >  2:3818051/1‑93 (MQ=255)

                         gctgctTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAatat                                                                  <  2:151235/93‑1 (MQ=255)

                          ctgctTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATg                                                                 >  2:2859030/1‑93 (MQ=255)

                            gctTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATgtc                                                               >  1:5401185/1‑91 (MQ=255)

                            gctTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGcc                                                               <  1:954819/93‑1 (MQ=255)

                            gctTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGcc                                                               <  1:3470016/93‑1 (MQ=255)

                             ctTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCcg                                                              >  2:5468451/1‑93 (MQ=255)

                               tGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCcgcg                                                            <  2:1125991/93‑1 (MQ=255)

                               tGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCcgcg                                                            <  1:5579189/93‑1 (MQ=255)

                                gggAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCgcgc                                                           >  1:1808691/1‑93 (MQ=255)

                                gggAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCgcgc                                                           <  2:102357/93‑1 (MQ=255)

                                gggAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCgcgc                                                           <  2:3901079/93‑1 (MQ=255)

                                 ggAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCt                                                          >  2:1830645/1‑93 (MQ=255)

                                 ggAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCt                                                          >  2:4278209/1‑93 (MQ=255)

                                   aaaaCGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGt                                                        <  1:1829614/93‑1 (MQ=255)

                                   aaaaCGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGt                                                        >  1:1967525/1‑93 (MQ=255)

                                   aaaaCGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGt                                                        <  1:5034762/93‑1 (MQ=255)

                                     aaCGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTaa                                                      <  2:4629083/93‑1 (MQ=255)

                                      aCGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAAc                                                     <  2:4039108/93‑1 (MQ=255)

                                      aCGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAAc                                                     <  2:5575305/93‑1 (MQ=255)

                                       cGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACg                                                    >  2:3734852/1‑93 (MQ=255)

                                       cGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACg                                                    <  2:1296823/93‑1 (MQ=255)

                                       cGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACg                                                    <  2:607375/93‑1 (MQ=255)

                                        ggAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACgg                                                   >  2:4250092/1‑93 (MQ=255)

                                        ggAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACgg                                                   >  2:2779044/1‑93 (MQ=255)

                                           aaCTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCaa                                                >  2:3435215/1‑93 (MQ=255)

                                           aaCTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCaa                                                >  2:2083329/1‑93 (MQ=255)

                                            aCTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCaaa                                               >  1:4632719/1‑93 (MQ=255)

                                            aCTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCaaa                                               <  2:2345308/93‑1 (MQ=255)

                                             cTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAg                                              <  2:4476901/93‑1 (MQ=255)

                                                cGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCtg                                           <  2:5629189/93‑1 (MQ=255)

                                                 gCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCtgt                                          >  1:1164630/1‑93 (MQ=255)

                                                  ccTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCtgtg                                         >  2:4559647/1‑93 (MQ=255)

                                                   cTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGc                                        >  2:6590897/1‑93 (MQ=255)

                                                   cTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGc                                        >  1:657987/1‑93 (MQ=255)

                                                    tGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGcc                                       <  1:2456963/93‑1 (MQ=255)

                                                    tGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGcc                                       <  1:1074989/93‑1 (MQ=255)

                                                    tGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGcc                                       <  1:4253316/93‑1 (MQ=255)

                                                    tGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGcc                                       >  1:2115394/1‑93 (MQ=255)

                                                       aCTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCcaca                                    <  2:1230778/93‑1 (MQ=255)

                                                         ttGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAg                                  >  2:5029839/1‑93 (MQ=255)

                                                           gAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTa                                <  1:4362459/93‑1 (MQ=255)

                                                            aGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAg                               <  1:3737794/93‑1 (MQ=255)

                                                            aGGTCTATCGTTTGTCCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAg                               <  2:2048432/93‑1 (MQ=255)

                                                              gTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGGAGAt                             >  1:1303607/1‑93 (MQ=255)

                                                               tCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATg                            >  2:3656623/1‑93 (MQ=255)

                                                                cTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGc                           >  1:3075291/1‑93 (MQ=255)

                                                                cTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGc                           >  1:2983303/1‑93 (MQ=255)

                                                                  aTCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGcac                         >  1:2687566/1‑93 (MQ=255)

                                                                   tCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGcaca                        >  2:6418090/1‑93 (MQ=255)

                                                                   tCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGcaca                        >  2:6447381/1‑93 (MQ=255)

                                                                   tCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGcaca                        <  2:4753320/93‑1 (MQ=255)

                                                                    cGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCacaa                       <  1:4686493/93‑1 (MQ=255)

                                                                     gTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCacaac                      >  1:5714582/1‑93 (MQ=255)

                                                                     gTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCacaac                      >  2:5684717/1‑93 (MQ=255)

                                                                     gTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCacaac                      <  2:1863021/93‑1 (MQ=255)

                                                                     gTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTATGCCACAAGTAGATGCacaac                      <  1:4227462/93‑1 (MQ=255)

                                                                        tGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAgg                   >  1:6281581/1‑93 (MQ=255)

                                                                         gTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGc                  <  1:3580273/93‑1 (MQ=255)

                                                                          ttCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCt                 <  2:198426/93‑1 (MQ=255)

                                                                          ttCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCt                 >  1:2752398/1‑93 (MQ=255)

                                                                          ttCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCt                 >  1:485399/1‑93 (MQ=255)

                                                                           tCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTa                <  1:2594484/93‑1 (MQ=255)

                                                                              aGCCAATTTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTAttt             >  2:2694351/1‑93 (MQ=255)

                                                                                ccAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTAttttt           >  1:73440/1‑93 (MQ=255)

                                                                                ccAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGGACAACAGGCTAttttt           >  1:593707/1‑93 (MQ=255)

                                                                                ccAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTAttttt           <  2:5916530/93‑1 (MQ=255)

                                                                                 cAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTa          <  2:1458867/93‑1 (MQ=255)

                                                                                 cAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTa          <  2:3600739/93‑1 (MQ=255)

                                                                                  aaTGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTAt         >  2:874731/1‑93 (MQ=255)

                                                                                       cAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCg    <  2:6127027/93‑1 (MQ=255)

                                                                                        aGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGt   >  2:481443/1‑93 (MQ=255)

                                                                                        aGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGt   <  2:107169/93‑1 (MQ=255)

                                                                                        aGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGt   <  2:905338/93‑1 (MQ=255)

                                                                                         gcgcCCTGGCGGCATGTAATGCACAAGATGCCGCGCTGTAACGGCAAAGCTGTGCCTCAAGTAGATGCACAACAGGCTATTTTTATCGCCGtt  <  2:5098254/93‑1 (MQ=255)

                                                                                         gcgcCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGtt  <  2:5287333/93‑1 (MQ=255)

AGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTT  >  NC_000913/4199877‑4200058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGAT  >  NC_000913/4199877‑4200061

AGCCAGAGGAAACCTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACC                                                                                             <  SRR4302547.1221930/94‑1 (MQ=60)

 GCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCC                                                                                            <  SRR4302547.5665659/94‑1 (MQ=60)

  CCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCT                                                                                           >  SRR4302547.2592947/1‑94 (MQ=60)

  CCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCT                                                                                           <  SRR4302547.662836/94‑1 (MQ=60)

  CCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCT                                                                                           <  SRR4302547.2420272/94‑1 (MQ=60)

  CCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCT                                                                                           >  SRR4302547.2271435/1‑94 (MQ=60)

  CCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCT                                                                                           <  SRR4302547.5113655/94‑1 (MQ=60)

    AGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGG                                                                                         >  SRR4302547.3491710/1‑94 (MQ=60)

    AGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGG                                                                                         >  SRR4302547.5071120/1‑94 (MQ=60)

    AGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGG                                                                                         >  SRR4302547.5387197/1‑94 (MQ=60)

     GAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGC                                                                                        <  SRR4302547.4585726/94‑1 (MQ=60)

       GGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGG                                                                                      >  SRR4302547.3522892/1‑94 (MQ=60)

       GGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGG                                                                                      >  SRR4302547.2761150/1‑94 (MQ=60)

       GGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGG                                                                                      <  SRR4302547.1241496/94‑1 (MQ=60)

        GAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGC                                                                                     >  SRR4302547.5891912/1‑94 (MQ=60)

         AAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCA                                                                                    >  SRR4302547.3765121/1‑94 (MQ=60)

          aatccCCCGCTGGCGGCTGCTTGAGAAAACGAAAACTGCGCCTGCCCTTGATGTCAATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCAT                                                                                   <  SRR4302547.1172850/89‑1 (MQ=60)

          AAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCAT                                                                                   >  SRR4302547.2717603/1‑94 (MQ=60)

          AAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCAT                                                                                   >  SRR4302547.2232280/1‑94 (MQ=60)

          AAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCAT                                                                                   >  SRR4302547.3894814/1‑94 (MQ=60)

           AACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATG                                                                                  >  SRR4302547.6510242/1‑94 (MQ=60)

            ACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGG                                                                                 <  SRR4302547.5671046/94‑1 (MQ=60)

               CCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAAT                                                                              <  SRR4302547.1752701/94‑1 (MQ=60)

               CCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAAT                                                                              >  SRR4302547.1462469/1‑94 (MQ=60)

                CCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATG                                                                             <  SRR4302547.181425/94‑1 (MQ=60)

                CCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATG                                                                             >  SRR4302547.5396010/1‑94 (MQ=60)

                CCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATG                                                                             <  SRR4302547.4728344/94‑1 (MQ=60)

                 CGCTGGCGGCTGCTTGGGAAAACGGAAACTACCCCGGCACTTGAGGTCAACCGATTTTTCCAGCCAATGTGAGAACCCTGGAGGCATGGAATGC                                                                            >  SRR4302547.5933410/1‑94 (MQ=42)

                  GCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCA                                                                           <  SRR4302547.447386/94‑1 (MQ=60)

                  GCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCA                                                                           <  SRR4302547.1632808/94‑1 (MQ=60)

                   CTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCAC                                                                          >  SRR4302547.2928573/1‑94 (MQ=60)

                   CTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCAC                                                                          >  SRR4302547.4837862/1‑94 (MQ=60)

                       CGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATA                                                                      >  SRR4302547.2176392/1‑94 (MQ=60)

                        GGCTGCTTGGGAAAACGTAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCGCAATAT                                                                     <  SRR4302547.6451315/94‑1 (MQ=60)

                         GCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATG                                                                    >  SRR4302547.3008380/1‑94 (MQ=60)

                          CTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGC                                                                   >  SRR4302547.5396408/1‑94 (MQ=60)

                          CTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGC                                                                   >  SRR4302547.1232269/1‑94 (MQ=60)

                           TGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGTC                                                                  >  SRR4302547.5512818/1‑94 (MQ=60)

                           TGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCC                                                                  >  SRR4302547.438777/1‑94 (MQ=60)

                            GCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCG                                                                 <  SRR4302547.3540785/94‑1 (MQ=60)

                            GCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCG                                                                 <  SRR4302547.742674/94‑1 (MQ=60)

                            GCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCG                                                                 <  SRR4302547.973370/94‑1 (MQ=60)

                            GCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCG                                                                 <  SRR4302547.2893685/94‑1 (MQ=60)

                              TTGGGAAACCGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCG                                                               <  SRR4302547.3053870/94‑1 (MQ=60)

                              TTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCG                                                               >  SRR4302547.3593552/1‑94 (MQ=60)

                               TGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGC                                                              >  SRR4302547.1844432/1‑94 (MQ=60)

                               TGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGC                                                              <  SRR4302547.5694241/94‑1 (MQ=60)

                               TGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGC                                                              <  SRR4302547.1911799/94‑1 (MQ=60)

                                 GGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTG                                                            <  SRR4302547.1670060/94‑1 (MQ=60)

                                  GAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGT                                                           >  SRR4302547.2006602/1‑94 (MQ=60)

                                  GAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGT                                                           <  SRR4302547.4508107/94‑1 (MQ=60)

                                   aaaccgcaaacgatGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTA                                                          <  SRR4302547.4772548/80‑1 (MQ=60)

                                   AAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTA                                                          <  SRR4302547.5138765/94‑1 (MQ=60)

                                   AAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTA                                                          <  SRR4302547.1865777/94‑1 (MQ=60)

                                     AACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAAC                                                        <  SRR4302547.2152928/94‑1 (MQ=60)

                                      ACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGAGGCATGGAATGCACAATATGCCGCGCTGTAACG                                                       >  SRR4302547.462326/1‑94 (MQ=60)

                                        GGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGC                                                     >  SRR4302547.2006268/1‑94 (MQ=60)

                                        GGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGC                                                     >  SRR4302547.3665706/1‑94 (MQ=60)

                                         GAAGCTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCA                                                    <  SRR4302547.1277479/94‑1 (MQ=60)

                                           AACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAA                                                  >  SRR4302547.4728676/1‑94 (MQ=60)

                                            ACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAG                                                 >  SRR4302547.2583340/1‑94 (MQ=60)

                                            ACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAG                                                 <  SRR4302547.178444/94‑1 (MQ=60)

                                              TACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCT                                               <  SRR4302547.2164428/94‑1 (MQ=60)

                                                CGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGT                                             >  SRR4302547.1188185/1‑94 (MQ=60)

                                                 GCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTG                                            >  SRR4302547.2296144/1‑94 (MQ=60)

                                                  CCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGC                                           >  SRR4302547.4759540/1‑94 (MQ=60)

                                                  CCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGC                                           >  SRR4302547.3539501/1‑94 (MQ=60)

                                                  CCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGC                                           >  SRR4302547.670105/1‑94 (MQ=60)

                                                   CTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCC                                          >  SRR4302547.2157544/1‑94 (MQ=60)

                                                    TGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCA                                         <  SRR4302547.2506617/94‑1 (MQ=60)

                                                    TGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCA                                         <  SRR4302547.1096444/94‑1 (MQ=60)

                                                    TGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCA                                         <  SRR4302547.4340345/94‑1 (MQ=60)

                                                     GCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCAC                                        >  SRR4302547.3188347/1‑94 (MQ=60)

                                                      CACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACA                                       <  SRR4302547.4872144/94‑1 (MQ=60)

                                                       ACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAA                                      >  SRR4302547.5765970/1‑94 (MQ=60)

                                                          TGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTA                                   >  SRR4302547.1008853/1‑94 (MQ=60)

                                                           GAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAG                                  <  SRR4302547.4452049/94‑1 (MQ=60)

                                                           GAGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAG                                  <  SRR4302547.3963836/94‑1 (MQ=60)

                                                            AGGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGA                                 <  SRR4302547.3814110/94‑1 (MQ=60)

                                                             GGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGAT                                <  SRR4302547.6233215/94‑1 (MQ=60)

                                                             GGTCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGGAGAT                                >  SRR4302547.1329833/1‑94 (MQ=60)

                                                               TCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGC                              >  SRR4302547.1859225/1‑94 (MQ=60)

                                                               TCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGC                              <  SRR4302547.2461119/94‑1 (MQ=60)

                                                               TCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGC                              >  SRR4302547.3136381/1‑94 (MQ=60)

                                                               TCTATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGC                              >  SRR4302547.3042673/1‑94 (MQ=60)

                                                                CTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCA                             <  SRR4302547.212130/94‑1 (MQ=60)

                                                                 TATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCAC                            >  SRR4302547.2741514/1‑94 (MQ=60)

                                                                  ATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTCGATGCACA                           <  SRR4302547.6445228/94‑1 (MQ=60)

                                                                  ATCGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACA                           >  SRR4302547.665883/1‑94 (MQ=60)

                                                                    CGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAAC                         <  SRR4302547.4783568/94‑1 (MQ=60)

                                                                    CGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAAC                         >  SRR4302547.5832609/1‑94 (MQ=60)

                                                                    CGTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAAC                         >  SRR4302547.2237872/1‑94 (MQ=60)

                                                                     GTTTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTATGCCACAAGTAGATGCACAACA                        <  SRR4302547.4313868/94‑1 (MQ=60)

                                                                       TTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGG                      >  SRR4302547.6412090/1‑94 (MQ=60)

                                                                       TTGTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGG                      <  SRR4302547.610241/94‑1 (MQ=60)

                                                                        TGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGC                     <  SRR4302547.5908557/94‑1 (MQ=60)

                                                                        TGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGC                     >  SRR4302547.1698300/1‑94 (MQ=60)

                                                                         GTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCT                    >  SRR4302547.2807581/1‑94 (MQ=60)

                                                                         GTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCT                    >  SRR4302547.494289/1‑94 (MQ=60)

                                                                         GTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCT                    >  SRR4302547.2867330/1‑94 (MQ=60)

                                                                         GTTCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCT                    <  SRR4302547.3653312/94‑1 (MQ=60)

                                                                          TTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTA                   <  SRR4302547.3040381/94‑1 (MQ=60)

                                                                           TCCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTAT                  <  SRR4302547.2646802/94‑1 (MQ=60)

                                                                            CCAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATT                 >  SRR4302547.2680896/1‑94 (MQ=60)

                                                                             CAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTT                <  SRR4302547.888490/94‑1 (MQ=60)

                                                                             CAGCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTT                <  SRR4302547.5326973/94‑1 (MQ=60)

                                                                               GCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTT              >  SRR4302547.74821/1‑94 (MQ=60)

                                                                               GCCAATGTCAGCACCGTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTT              <  SRR4302547.3173235/94‑1 (MQ=60)

                                                                               GCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGGACAACAGGCTATTTTT              >  SRR4302547.604563/1‑94 (MQ=60)

                                                                               GCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTT              >  SRR4302547.229189/1‑94 (MQ=60)

                                                                               GCCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTT              >  SRR4302547.1138537/1‑94 (MQ=60)

                                                                               GCCAATGTCAGCACCCTGGAGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTT              >  SRR4302547.6538823/1‑94 (MQ=60)

                                                                                CCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTA             >  SRR4302547.4319918/1‑94 (MQ=60)

                                                                                CCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTA             >  SRR4302547.1197632/1‑94 (MQ=60)

                                                                                CCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTA             <  SRR4302547.6678762/94‑1 (MQ=60)

                                                                                CCAATGTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTA             >  SRR4302547.903702/1‑94 (MQ=60)

                                                                                     GTCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCC        >  SRR4302547.4320673/1‑94 (MQ=60)

                                                                                      TCAGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCG       >  SRR4302547.1287143/1‑94 (MQ=60)

                                                                                        AGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTT     >  SRR4302547.2493425/1‑94 (MQ=60)

                                                                                        AGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTT     >  SRR4302547.4925442/1‑94 (MQ=60)

                                                                                        AGCACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTT     >  SRR4302547.4418464/1‑94 (MQ=60)

                                                                                           GCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGAT  >  SRR4302547.4486445/1‑94 (MQ=60)

                                                                                           ACCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGAT  <  SRR4302547.5998342/94‑1 (MQ=60)

AGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGAT  >  NC_000913/4199877‑4200061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`