Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,224,890 G→A G1162S (GGC→AGC)  metH → B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,224,8900GA84.2% 732.6 / 104.7 265G1162S (GGC→AGC) metHB12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand):  ref base G (16/26);  new base A (126/97);  total (142/123)
Fisher's exact test for biased strand distribution p-value = 4.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

Only 100 of 270 total aligned reads displayed.

AGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTA  >  NC_000913/4224798‑4224982
                                                                                            |                                                                                            
  ggCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGc                                                                                            <  2:6073704/93‑1 (MQ=255)
  ggCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGc                                                                                            <  2:73939/93‑1 (MQ=255)
   gCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCa                                                                                           <  1:4021826/93‑1 (MQ=255)
   gCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCa                                                                                           <  2:3541738/93‑1 (MQ=255)
      tccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGa                                                                                        <  2:4604250/93‑1 (MQ=255)
      tccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGa                                                                                        >  2:5130321/1‑93 (MQ=255)
      tccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGa                                                                                        >  2:5418878/1‑93 (MQ=255)
      tccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGa                                                                                        >  1:3100215/1‑93 (MQ=255)
      tccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGa                                                                                        >  2:261783/1‑93 (MQ=255)
      tccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGa                                                                                        >  2:508971/1‑93 (MQ=255)
      tccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGa                                                                                        >  1:4287468/1‑93 (MQ=255)
       ccgtccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGaa                                                                                       <  1:1847065/93‑1 (MQ=255)
          tccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACt                                                                                    <  1:5129218/93‑1 (MQ=255)
          tccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACt                                                                                    >  1:718015/1‑93 (MQ=255)
          tccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCCGCTGGAAGTGGAAAAACACACTGGCATGAAACt                                                                                    >  1:6114331/1‑93 (MQ=255)
           ccgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTc                                                                                   <  1:4086217/93‑1 (MQ=255)
            cgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTca                                                                                  <  2:1177029/93‑1 (MQ=255)
            cgGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTca                                                                                  >  2:2455884/1‑93 (MQ=255)
             ggCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGCAGTGGAAAAACACACTAGCATGAAACTcac                                                                                 >  2:4913054/1‑93 (MQ=255)
             ggCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTcac                                                                                 <  1:2673409/93‑1 (MQ=255)
             ggCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTcac                                                                                 >  2:2506911/1‑93 (MQ=255)
              gCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTcaca                                                                                >  1:3123523/1‑93 (MQ=255)
                aCCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGa                                                                              <  2:6170075/93‑1 (MQ=255)
                 ccGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGaa                                                                             <  1:4466800/93‑1 (MQ=255)
                  cGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAAt                                                                            <  1:5720721/93‑1 (MQ=255)
                   gggCTATCCGGCCTGCCCGGAACATACGGAAAAATCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATc                                                                           >  1:1564181/1‑93 (MQ=255)
                   gggCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATc                                                                           <  1:1137486/93‑1 (MQ=255)
                   gggCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATc                                                                           <  1:351671/93‑1 (MQ=255)
                    ggCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCt                                                                          >  1:1821708/1‑93 (MQ=255)
                     gCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCtt                                                                         <  1:2582118/93‑1 (MQ=255)
                     gCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCtt                                                                         >  2:442813/1‑93 (MQ=255)
                      cTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCttt                                                                        <  1:1247789/93‑1 (MQ=255)
                      cTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCttt                                                                        <  1:5743223/93‑1 (MQ=255)
                       tATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTc                                                                       <  1:255008/93‑1 (MQ=255)
                        aTCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCg                                                                      >  1:2900015/1‑93 (MQ=255)
                          ccGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGcc                                                                    <  1:3855085/93‑1 (MQ=255)
                          ccGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGcc                                                                    <  1:6218958/93‑1 (MQ=255)
                            ggCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCAt                                                                  >  1:3302095/1‑93 (MQ=255)
                             gCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCAtg                                                                 >  2:3001701/1‑93 (MQ=255)
                               cTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCAtgtg                                                               >  1:616392/1‑93 (MQ=255)
                                tGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTgg                                                              >  2:25251/1‑93 (MQ=255)
                                tGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTgg                                                              >  2:3384104/1‑93 (MQ=255)
                                   ccGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGccc                                                           >  1:3775835/1‑93 (MQ=255)
                                   ccGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGccc                                                           >  1:2522303/1‑93 (MQ=255)
                                    cGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCg                                                          >  1:1301524/1‑93 (MQ=255)
                                    cGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCg                                                          >  2:109210/1‑93 (MQ=255)
                                     ggAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCgg                                                         >  2:3785813/1‑93 (MQ=255)
                                      gAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGt                                                        >  1:883523/1‑93 (MQ=255)
                                       aaCATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTg                                                       <  1:2041009/93‑1 (MQ=255)
                                       aaCATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTg                                                       >  1:3221065/1‑93 (MQ=255)
                                       aaCATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTg                                                       >  1:278595/1‑93 (MQ=255)
                                        aCATACGGAAAAAGCCACCATCTGAGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGc                                                      <  2:4995144/93‑1 (MQ=255)
                                         cATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCa                                                     <  1:4799401/93‑1 (MQ=255)
                                         cATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCa                                                     <  2:1426661/93‑1 (MQ=255)
                                           tACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATc                                                   <  1:4952635/93‑1 (MQ=255)
                                           tACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATc                                                   >  1:4283130/1‑93 (MQ=255)
                                           tACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATc                                                   <  1:785775/93‑1 (MQ=255)
                                           tACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATc                                                   >  2:5723648/1‑93 (MQ=255)
                                             cGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCgg                                                 >  2:6400985/1‑93 (MQ=255)
                                             cGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCgg                                                 >  2:4278506/1‑93 (MQ=255)
                                              ggAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGt                                                <  1:1889030/93‑1 (MQ=255)
                                               gAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGtt                                               <  2:1877231/93‑1 (MQ=255)
                                               gAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGtt                                               >  1:1390668/1‑93 (MQ=255)
                                               gAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGtt                                               <  1:5583058/93‑1 (MQ=255)
                                                 aaaaGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTc                                             >  1:2479510/1‑93 (MQ=255)
                                                    aGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCggg                                          >  2:5449754/1‑93 (MQ=255)
                                                     gCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGt                                         >  2:4766690/1‑93 (MQ=255)
                                                     gCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGACCGGTGCATCGGTTTCGGGt                                         >  1:5656324/1‑93 (MQ=255)
                                                     accaccaTCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGt                                         <  2:5593254/92‑1 (MQ=255)
                                                      ccaccaTCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGtt                                        >  2:867161/1‑93 (MQ=255)
                                                       caccaTCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTg                                       >  1:959/1‑93 (MQ=255)
                                                         ccaTCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGt                                     <  2:3099037/93‑1 (MQ=255)
                                                           aTCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTAc                                   <  2:6447985/93‑1 (MQ=255)
                                                             cTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACtt                                 <  2:6514881/93‑1 (MQ=255)
                                                             cTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACtt                                 >  1:4271660/1‑93 (MQ=255)
                                                              tGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTc                                >  2:3493934/1‑93 (MQ=255)
                                                               gggAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCa                               >  2:1611202/1‑93 (MQ=255)
                                                                ggAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAg                              >  2:2160016/1‑93 (MQ=255)
                                                                ggAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAg                              >  2:2096110/1‑93 (MQ=255)
                                                                  aGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGcc                            >  2:5976467/1‑93 (MQ=255)
                                                                  aGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGcc                            <  1:602409/93‑1 (MQ=255)
                                                                    ctgctgGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCAc                          <  1:2844039/93‑1 (MQ=255)
                                                                    ctgctgGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCAc                          <  2:3329165/93‑1 (MQ=255)
                                                                        tgGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCgg                      >  1:3622698/1‑93 (MQ=255)
                                                                         ggAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGa                     <  2:5207876/93‑1 (MQ=255)
                                                                          gAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGAc                    <  2:2858581/93‑1 (MQ=255)
                                                                           aaGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACa                   >  1:2369470/1‑93 (MQ=255)
                                                                            aGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAg                  <  1:6343241/93‑1 (MQ=255)
                                                                                gAAAAACACACTAGCATGAAACTCACAGAATGTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAg              >  1:5308358/1‑93 (MQ=255)
                                                                                gAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAg              >  1:5384822/1‑93 (MQ=255)
                                                                                gAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAg              <  2:2268454/93‑1 (MQ=255)
                                                                                 aaaaaCACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGt             >  1:4645016/1‑93 (MQ=255)
                                                                                   aaaCACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGtac           >  2:3649175/1‑93 (MQ=255)
                                                                                     acacacTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGtacta         >  2:4022290/1‑93 (MQ=255)
                                                                                      cacacTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCCAGtactac        <  1:6493555/93‑1 (MQ=255)
                                                                                      cacacTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGtactac        >  1:108074/1‑93 (MQ=255)
                                                                                       acacTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACg       >  1:5027971/1‑93 (MQ=255)
                                                                                       acacTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACg       >  1:5474815/1‑93 (MQ=255)
                                                                                       acacTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACg       >  2:1954370/1‑93 (MQ=255)
                                                                                        cacTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGc      <  1:2266674/93‑1 (MQ=255)
                                                                                            |                                                                                            
AGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTA  >  NC_000913/4224798‑4224982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAG  >  NC_000913/4224797‑4224983
                                                                                             |                                                                                             
CAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTG                                                                                               <  SRR4302547.1195718/94‑1 (MQ=60)
CAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTA                                                                                               <  SRR4302547.5854025/94‑1 (MQ=60)
 AGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAG                                                                                              >  SRR4302547.427837/1‑94 (MQ=60)
 AGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAG                                                                                              <  SRR4302547.5452225/94‑1 (MQ=60)
   GGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCA                                                                                            >  SRR4302547.2596521/1‑94 (MQ=60)
    GCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCAT                                                                                           <  SRR4302547.4103545/94‑1 (MQ=60)
     CATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATG                                                                                          <  SRR4302547.1850992/94‑1 (MQ=60)
     CATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATG                                                                                          <  SRR4302547.1238730/94‑1 (MQ=60)
      ATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGA                                                                                         >  SRR4302547.3161620/1‑94 (MQ=60)
      ATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGA                                                                                         >  SRR4302547.4375586/1‑94 (MQ=60)
      ATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGA                                                                                         >  SRR4302547.5641697/1‑94 (MQ=60)
      ATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGA                                                                                         >  SRR4302547.2699888/1‑94 (MQ=60)
       TCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAA                                                                                        >  SRR4302547.3673823/1‑94 (MQ=60)
        CCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAA                                                                                       <  SRR4302547.1883580/94‑1 (MQ=60)
          GTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACT                                                                                     >  SRR4302547.731333/1‑94 (MQ=60)
          GTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCCGCTGGAAGTGGAAAAACACACTGGCATGAAACT                                                                                     >  SRR4302547.6241166/1‑94 (MQ=60)
           TCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTC                                                                                    >  SRR4302547.810410/1‑94 (MQ=60)
           TCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTC                                                                                    <  SRR4302547.5234908/94‑1 (MQ=60)
            CCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCA                                                                                   <  SRR4302547.2519554/94‑1 (MQ=60)
            CCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCA                                                                                   <  SRR4302547.4169273/94‑1 (MQ=60)
              GGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACA                                                                                 <  SRR4302547.916110/94‑1 (MQ=60)
              GGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACA                                                                                 >  SRR4302547.3185474/1‑94 (MQ=60)
              GGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACA                                                                                 <  SRR4302547.2727110/94‑1 (MQ=60)
                 ACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAA                                                                              <  SRR4302547.3696677/94‑1 (MQ=60)
                 ACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAA                                                                              >  SRR4302547.5466636/1‑94 (MQ=60)
                  CCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAAT                                                                             <  SRR4302547.4559097/94‑1 (MQ=60)
                  CCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAAT                                                                             <  SRR4302547.483781/94‑1 (MQ=60)
                   CGGGCTATCCGGCCTGCCCGGAACATACGGAAAAATCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATC                                                                            >  SRR4302547.1595336/1‑94 (MQ=60)
                   CGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCCTCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCAAAGAATC                                                                            >  SRR4302547.5157681/1‑94 (MQ=60)
                   CGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATC                                                                            >  SRR4302547.5343366/1‑94 (MQ=60)
                   CGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATC                                                                            >  SRR4302547.4852138/1‑94 (MQ=60)
                   CGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATC                                                                            <  SRR4302547.5838900/94‑1 (MQ=60)
                    GGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCT                                                                           <  SRR4302547.1160457/94‑1 (MQ=60)
                    GGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCT                                                                           >  SRR4302547.1857721/1‑94 (MQ=60)
                    GGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCT                                                                           <  SRR4302547.1128181/94‑1 (MQ=60)
                    GGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCT                                                                           <  SRR4302547.6721104/94‑1 (MQ=60)
                    GGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCT                                                                           <  SRR4302547.358202/94‑1 (MQ=60)
                     GGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTT                                                                          >  SRR4302547.2349948/1‑94 (MQ=60)
                      GCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTT                                                                         <  SRR4302547.2634231/94‑1 (MQ=60)
                      GCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTT                                                                         <  SRR4302547.535357/94‑1 (MQ=60)
                      GCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTT                                                                         >  SRR4302547.2503962/1‑94 (MQ=60)
                      ACTATCCGGCCTGCCCGGGACATACGGAAAAAGCCACCCTCTGGGAGCTGCTGGAAGTGGCAAAACACACTAGGATGAAACTCACAGAATCTTT                                                                         >  SRR4302547.5056039/1‑94 (MQ=60)
                       CTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTC                                                                        <  SRR4302547.1272974/94‑1 (MQ=60)
                       CTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTC                                                                        >  SRR4302547.436529/1‑94 (MQ=60)
                       CTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTC                                                                        <  SRR4302547.5861904/94‑1 (MQ=60)
                       CTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTC                                                                        >  SRR4302547.1928918/1‑94 (MQ=60)
                       CTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTC                                                                        >  SRR4302547.2885236/1‑94 (MQ=60)
                        TATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCG                                                                       <  SRR4302547.2713650/94‑1 (MQ=60)
                        TATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCG                                                                       <  SRR4302547.259778/94‑1 (MQ=60)
                        TATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCG                                                                       >  SRR4302547.2957847/1‑94 (MQ=60)
                         ATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGC                                                                      >  SRR4302547.2609626/1‑94 (MQ=60)
                         ATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGC                                                                      >  SRR4302547.4631184/1‑94 (MQ=60)
                           CCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCA                                                                    <  SRR4302547.6348109/94‑1 (MQ=60)
                           CCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCA                                                                    <  SRR4302547.3933556/94‑1 (MQ=60)
                           CCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCA                                                                    <  SRR4302547.5859946/94‑1 (MQ=60)
                            TGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCAT                                                                   >  SRR4302547.3368571/1‑94 (MQ=60)
                             GGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGCCATGAACCTCATAGAATCTTTCGCCATG                                                                  >  SRR4302547.1911587/1‑94 (MQ=60)
                             GGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCTATG                                                                  <  SRR4302547.3021582/94‑1 (MQ=60)
                              GCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGT                                                                 >  SRR4302547.2532276/1‑94 (MQ=60)
                               CCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTG                                                                >  SRR4302547.5722200/1‑94 (MQ=60)
                               CCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTG                                                                >  SRR4302547.627811/1‑94 (MQ=60)
                               CCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTG                                                                >  SRR4302547.3230214/1‑94 (MQ=60)
                                 TGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTAGCCATGTGGC                                                              >  SRR4302547.5041046/1‑94 (MQ=60)
                                  GCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCC                                                             >  SRR4302547.2163009/1‑94 (MQ=60)
                                  GCACGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCC                                                             >  SRR4302547.6492311/1‑94 (MQ=60)
                                  GACCGAAACATACGGAAAAAGCCACAATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCC                                                             <  SRR4302547.1934128/94‑1 (MQ=60)
                                   CCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCC                                                            <  SRR4302547.1802145/94‑1 (MQ=60)
                                   CCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCC                                                            >  SRR4302547.2573275/1‑94 (MQ=60)
                                   CCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCC                                                            >  SRR4302547.1495645/1‑94 (MQ=60)
                                   CCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCC                                                            >  SRR4302547.3852842/1‑94 (MQ=60)
                                   CCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCC                                                            >  SRR4302547.5716216/1‑94 (MQ=60)
                                    CCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGACCCTCCCAGACTCTTTCGCCATGTGGCCCG                                                           <  SRR4302547.277352/94‑1 (MQ=60)
                                    CCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCG                                                           >  SRR4302547.1327711/1‑94 (MQ=60)
                                     CGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGG                                                          >  SRR4302547.1012360/1‑94 (MQ=60)
                                     CGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGG                                                          <  SRR4302547.3094820/94‑1 (MQ=60)
                                      GGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGT                                                         <  SRR4302547.5204624/94‑1 (MQ=60)
                                      GGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGT                                                         >  SRR4302547.900505/1‑94 (MQ=60)
                                       GAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTG                                                        >  SRR4302547.283788/1‑94 (MQ=60)
                                       GAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTG                                                        >  SRR4302547.3285316/1‑94 (MQ=60)
                                        tgtgtttttccacttCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGC                                                       >  SRR4302547.219819/16‑94 (MQ=60)
                                        AACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGC                                                       >  SRR4302547.1320815/1‑94 (MQ=60)
                                        AACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGC                                                       <  SRR4302547.2081715/94‑1 (MQ=60)
                                         ACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCA                                                      <  SRR4302547.3175424/94‑1 (MQ=60)
                                          CATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCAT                                                     <  SRR4302547.4898848/94‑1 (MQ=60)
                                           ATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATC                                                    >  SRR4302547.4371119/1‑94 (MQ=60)
                                           ATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATC                                                    <  SRR4302547.5786514/94‑1 (MQ=60)
                                            TACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCG                                                   <  SRR4302547.5055170/94‑1 (MQ=60)
                                            TACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCG                                                   <  SRR4302547.800642/94‑1 (MQ=60)
                                            TACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCG                                                   <  SRR4302547.4469354/94‑1 (MQ=60)
                                              CGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGT                                                 >  SRR4302547.5655319/1‑94 (MQ=60)
                                               GGAAAAAGCCCCCCTCTGGGATCTGCTGGAAGTTGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGTCCCGGTGCATCGGTT                                                <  SRR4302547.879062/94‑1 (MQ=60)
                                               GGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTT                                                <  SRR4302547.1926362/94‑1 (MQ=60)
                                               GGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTT                                                >  SRR4302547.1418501/1‑94 (MQ=60)
                                                GAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTT                                               <  SRR4302547.5698215/94‑1 (MQ=60)
                                                GAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTT                                               <  SRR4302547.2941443/94‑1 (MQ=60)
                                                 AAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTC                                              >  SRR4302547.2529626/1‑94 (MQ=60)
                                                 AAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTC                                              <  SRR4302547.1860405/94‑1 (MQ=60)
                                                  AAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCG                                             >  SRR4302547.1302855/1‑94 (MQ=60)
                                                  AAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCG                                             >  SRR4302547.548943/1‑94 (MQ=60)
                                                   AAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGG                                            >  SRR4302547.6219139/1‑94 (MQ=60)
                                                    AAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGG                                           >  SRR4302547.253995/1‑94 (MQ=60)
                                                    AAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGG                                           <  SRR4302547.4247790/94‑1 (MQ=60)
                                                     AGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGT                                          <  SRR4302547.75545/94‑1 (MQ=60)
                                                     AGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGT                                          >  SRR4302547.800622/1‑94 (MQ=60)
                                                     AGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGACCGGTGCATCGGTTTCGGGT                                          >  SRR4302547.5773087/1‑94 (MQ=60)
                                                      GCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTT                                         <  SRR4302547.3583758/94‑1 (MQ=60)
                                                      GCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTT                                         <  SRR4302547.5002116/94‑1 (MQ=60)
                                                      GCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTT                                         >  SRR4302547.6407138/1‑94 (MQ=60)
                                                       CCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTG                                        >  SRR4302547.974/1‑94 (MQ=60)
                                                       CCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTG                                        <  SRR4302547.4142811/94‑1 (MQ=60)
                                                       CCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTG                                        >  SRR4302547.6261314/1‑94 (MQ=60)
                                                        CACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAACCTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGG                                       <  SRR4302547.587324/94‑1 (MQ=60)
                                                          CCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTA                                     >  SRR4302547.6432018/1‑94 (MQ=60)
                                                          CCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTA                                     <  SRR4302547.6063030/94‑1 (MQ=60)
                                                           CATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTAC                                    >  SRR4302547.2848402/1‑94 (MQ=60)
                                                            ATCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACT                                   >  SRR4302547.986041/1‑94 (MQ=60)
                                                             TCTGGGAGCTGCTGGAAGTGGAAAACCACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTT                                  <  SRR4302547.2613238/94‑1 (MQ=60)
                                                             TCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTT                                  >  SRR4302547.2122451/1‑94 (MQ=60)
                                                             TCTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTT                                  >  SRR4302547.4359339/1‑94 (MQ=60)
                                                              CTGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTC                                 <  SRR4302547.6242843/94‑1 (MQ=60)
                                                               TGGGAGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCA                                <  SRR4302547.4255978/94‑1 (MQ=60)
                                                                   AGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCA                            >  SRR4302547.3293289/1‑94 (MQ=60)
                                                                   AGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCA                            <  SRR4302547.4086946/94‑1 (MQ=60)
                                                                   AGCTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCA                            <  SRR4302547.613577/94‑1 (MQ=60)
                                                                     CTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACC                          <  SRR4302547.2900863/94‑1 (MQ=60)
                                                                     CTGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACC                          >  SRR4302547.730253/1‑94 (MQ=60)
                                                                      TGCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCC                         >  SRR4302547.1536496/1‑94 (MQ=60)
                                                                       GCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCG                        <  SRR4302547.3893126/94‑1 (MQ=60)
                                                                       GCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCG                        <  SRR4302547.949070/94‑1 (MQ=60)
                                                                       GCTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCG                        <  SRR4302547.436487/94‑1 (MQ=60)
                                                                        CTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGG                       >  SRR4302547.3729702/1‑94 (MQ=60)
                                                                        CTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGG                       <  SRR4302547.1203640/94‑1 (MQ=60)
                                                                        CTGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGG                       >  SRR4302547.3696603/1‑94 (MQ=60)
                                                                         TGGAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGA                      >  SRR4302547.189342/1‑94 (MQ=60)
                                                                          GGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGAC                     <  SRR4302547.3329289/94‑1 (MQ=60)
                                                                          GGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGAC                     <  SRR4302547.4425036/94‑1 (MQ=60)
                                                                           GAAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACA                    >  SRR4302547.2417499/1‑94 (MQ=60)
                                                                            AAGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAG                   >  SRR4302547.3616485/1‑94 (MQ=60)
                                                                             AGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGC                  >  SRR4302547.2916701/1‑94 (MQ=60)
                                                                             AGTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGC                  <  SRR4302547.6475341/94‑1 (MQ=60)
                                                                              GTGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGCCAGCA                 >  SRR4302547.3268811/1‑94 (MQ=60)
                                                                               TGGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCCGGCTGGTACTTCAGCCACCGGGACAGCAA                >  SRR4302547.6295202/1‑94 (MQ=60)
                                                                                GGAAAAACACACTAGCATGAAACTCACAGAATGTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAG               >  SRR4302547.5417843/1‑94 (MQ=60)
                                                                                GGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCATCCACCCGGACAGCAAG               >  SRR4302547.629498/1‑94 (MQ=60)
                                                                                GGAAAAACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAG               >  SRR4302547.5496009/1‑94 (MQ=60)
                                                                                 GAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGT              >  SRR4302547.4741209/1‑94 (MQ=60)
                                                                                    AAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACT           <  SRR4302547.545590/94‑1 (MQ=60)
                                                                                     AACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTA          >  SRR4302547.1375628/1‑94 (MQ=60)
                                                                                      ACACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTAC         >  SRR4302547.110048/1‑94 (MQ=60)
                                                                                       CACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCCAGTACTACG        <  SRR4302547.6628735/94‑1 (MQ=60)
                                                                                       CACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACG        <  SRR4302547.58339/94‑1 (MQ=60)
                                                                                       CACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACG        >  SRR4302547.5587903/1‑94 (MQ=60)
                                                                                       CACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACG        >  SRR4302547.5131849/1‑94 (MQ=60)
                                                                                        ACACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGC       >  SRR4302547.4040319/1‑94 (MQ=60)
                                                                                         CACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCT      <  SRR4302547.4136183/94‑1 (MQ=60)
                                                                                         CACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCT      <  SRR4302547.2312507/94‑1 (MQ=60)
                                                                                         CACTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCT      <  SRR4302547.1936474/94‑1 (MQ=60)
                                                                                           CTAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGT    <  SRR4302547.615914/94‑1 (MQ=60)
                                                                                            TGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTA   <  SRR4302547.4368199/94‑1 (MQ=60)
                                                                                            TAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTA   <  SRR4302547.3303489/94‑1 (MQ=60)
                                                                                            TAGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTA   <  SRR4302547.3247146/94‑1 (MQ=60)
                                                                                             AGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAG  <  SRR4302547.3701324/94‑1 (MQ=60)
                                                                                             AGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAG  >  SRR4302547.1459923/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAGGGCATCCGTCCGGCACCGGGCTATCCGGCCTGCCCGGAACATACGGAAAAAGCCACCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAG  >  NC_000913/4224797‑4224983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑