Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,227,714	A→G	L3P (CTG→CCG)	pepE ←	peptidase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,227,714	0	A	G	88.0%	729.1 / 82.2	266	L3P (CTG→CCG)	pepE	peptidase E
Reads supporting (aligned to +/- strand): ref base A (16/16); new base G (113/121); total (129/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

Only 100 of 267 total aligned reads displayed.

GAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCG  >  NC_000913/4227624‑4227803

gAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGt                                                                                         >  2:5729540/1‑93 (MQ=255)

 aGCGGCGACCCTGTAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGtt                                                                                        <  2:5183044/93‑1 (MQ=255)

  gcggcgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTc                                                                                       >  1:4880/1‑93 (MQ=255)

  gcggcgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTc                                                                                       <  1:2431455/93‑1 (MQ=255)

    ggcgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCa                                                                                     <  2:1672821/93‑1 (MQ=255)

     gcgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCAt                                                                                    <  2:510282/93‑1 (MQ=255)

     gcgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCAt                                                                                    <  2:5637744/93‑1 (MQ=255)

     gcgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGCCTTGACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCAt                                                                                    <  1:594680/93‑1 (MQ=255)

      cgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCAtt                                                                                   <  1:271027/93‑1 (MQ=255)

      cgACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCAtt                                                                                   >  1:843087/1‑93 (MQ=255)

        aCCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTAc                                                                                 <  2:3664022/93‑1 (MQ=255)

        aCCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTAc                                                                                 <  1:1328333/93‑1 (MQ=255)

        aCCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTAc                                                                                 >  1:315034/1‑93 (MQ=255)

         cccTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACt                                                                                >  2:5354648/1‑93 (MQ=255)

          ccTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACtt                                                                               >  2:719452/1‑93 (MQ=255)

           cTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTtc                                                                              <  1:70221/93‑1 (MQ=255)

           cTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTtc                                                                              <  1:913436/93‑1 (MQ=255)

           cTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTtc                                                                              >  2:3013302/1‑93 (MQ=255)

            tGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTtct                                                                             >  1:5867/1‑93 (MQ=255)

             gCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTtctc                                                                            >  1:1117202/1‑93 (MQ=255)

             gCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTtctc                                                                            >  2:5904977/1‑93 (MQ=255)

              cAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTcc                                                                           >  1:5690297/1‑93 (MQ=255)

               aaCTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCg                                                                          <  1:4615324/93‑1 (MQ=255)

               aaCTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCg                                                                          >  2:1892761/1‑93 (MQ=255)

                  tGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGtt                                                                       <  2:3155203/93‑1 (MQ=255)

                  tGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGtt                                                                       >  2:1958835/1‑93 (MQ=255)

                      cAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTa                                                                   >  2:2024575/1‑93 (MQ=255)

                           aTTAGCGGCAGTGCATGTTCCAGCCAGTCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGaaa                                                              <  2:554834/93‑1 (MQ=255)

                           aTTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGaaa                                                              <  2:4029419/93‑1 (MQ=255)

                            ttAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAAt                                                             >  1:5567965/1‑93 (MQ=255)

                            ttAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAAt                                                             <  2:2942442/93‑1 (MQ=255)

                             tAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATc                                                            <  1:4523455/93‑1 (MQ=255)

                              aGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCa                                                           <  2:65603/93‑1 (MQ=255)

                              aGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCa                                                           >  2:1148623/1‑93 (MQ=255)

                               gCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCAc                                                          <  2:585838/93‑1 (MQ=255)

                               gCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCAc                                                          <  1:2233061/93‑1 (MQ=255)

                               gCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCAc                                                          <  1:855480/93‑1 (MQ=255)

                                  gCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACggg                                                       >  2:3840413/1‑93 (MQ=255)

                                   cAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGa                                                      <  1:3592978/93‑1 (MQ=255)

                                    agggCATGTTCCAGCCAGGCTTTACCGGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAg                                                     <  2:1022343/90‑1 (MQ=255)

                                    aGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAg                                                     <  2:4570457/93‑1 (MQ=255)

                                     gTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAgg                                                    >  1:6435856/1‑93 (MQ=255)

                                     gTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAgg                                                    >  2:4729674/1‑93 (MQ=255)

                                      tGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGc                                                   >  1:4844670/1‑93 (MQ=255)

                                       gCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCa                                                  <  1:6292262/93‑1 (MQ=255)

                                        cATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCAt                                                 <  2:6467212/93‑1 (MQ=255)

                                        cATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCAt                                                 <  1:4775360/93‑1 (MQ=255)

                                         aTGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATg                                                >  2:3318098/1‑93 (MQ=255)

                                         aTGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATg                                                >  2:4455633/1‑93 (MQ=255)

                                           gTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCt                                              >  2:3716644/1‑93 (MQ=255)

                                             tCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTAc                                            >  1:5246095/1‑93 (MQ=255)

                                             tCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTAc                                            >  1:2557547/1‑93 (MQ=255)

                                               cagccagGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCa                                          >  1:6183132/1‑93 (MQ=255)

                                                 gccagGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACt                                        >  1:784250/1‑93 (MQ=255)

                                                 gccagGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACt                                        >  2:6032707/1‑93 (MQ=255)

                                                 gccagGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACt                                        >  1:1048071/1‑93 (MQ=255)

                                                  ccagGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTg                                       >  2:1312678/1‑93 (MQ=255)

                                                  ccagGCTTTACCCGGCAGCGTCGAGTGACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTg                                       <  1:3383519/93‑1 (MQ=255)

                                                   cagGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGc                                      <  1:616833/93‑1 (MQ=255)

                                                     ggCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGa                                    >  2:2253131/1‑93 (MQ=255)

                                                     ggCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGGTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGa                                    >  1:3970642/1‑93 (MQ=255)

                                                      gCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGaa                                   <  2:4951556/93‑1 (MQ=255)

                                                        tttACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAAtt                                 >  2:4079027/1‑93 (MQ=255)

                                                         ttACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAAttt                                <  1:1925641/93‑1 (MQ=255)

                                                            cccGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAc                             <  1:953482/93‑1 (MQ=255)

                                                            cccGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAc                             <  2:378696/93‑1 (MQ=255)

                                                             ccGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAcc                            >  1:5145543/1‑93 (MQ=255)

                                                                gCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACt                         <  1:1727666/93‑1 (MQ=255)

                                                                  aGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTa                       >  2:5285706/1‑93 (MQ=255)

                                                                  aGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTa                       >  2:4219002/1‑93 (MQ=255)

                                                                    cGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAGAACTTACt                     <  1:6539018/93‑1 (MQ=255)

                                                                    cGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACt                     <  1:4689228/93‑1 (MQ=255)

                                                                    cGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACt                     <  2:2248091/93‑1 (MQ=255)

                                                                     gTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTg                    >  2:786485/1‑93 (MQ=255)

                                                                     gTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTg                    >  2:6304893/1‑93 (MQ=255)

                                                                      tCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGa                   >  1:2202252/1‑93 (MQ=255)

                                                                       cGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGAc                  >  2:2080870/1‑93 (MQ=255)

                                                                        gAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACa                 >  2:5940677/1‑93 (MQ=255)

                                                                        gAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACa                 <  1:4236211/93‑1 (MQ=255)

                                                                         aGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAt                >  1:1652869/1‑93 (MQ=255)

                                                                         aGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAt                <  1:296253/93‑1 (MQ=255)

                                                                         aGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAt                >  2:3489934/1‑93 (MQ=255)

                                                                         aGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAt                <  1:280877/93‑1 (MQ=255)

                                                                          gTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATg               >  2:2513085/1‑93 (MQ=255)

                                                                            tACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGc             >  2:653665/1‑93 (MQ=255)

                                                                             aCTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGcc            >  1:2521132/1‑92 (MQ=255)

                                                                              cTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTc           <  1:1799411/93‑1 (MQ=255)

                                                                              cTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTc           <  1:729025/93‑1 (MQ=255)

                                                                              cTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTc           >  2:3946447/1‑93 (MQ=255)

                                                                               tCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCa          >  2:1636628/1‑93 (MQ=255)

                                                                               tCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCa          >  2:2789405/1‑93 (MQ=255)

                                                                                  aTAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGa       >  2:2663420/1‑93 (MQ=255)

                                                                                  aTAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGa       >  1:5322520/1‑93 (MQ=255)

                                                                                     aaaGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGt    >  2:3260861/1‑93 (MQ=255)

                                                                                     aaaGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGt    >  1:1481665/1‑93 (MQ=255)

                                                                                     aaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGt    <  2:2353352/93‑1 (MQ=255)

                                                                                      aaGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTc   >  1:2352625/1‑93 (MQ=255)

                                                                                      aaGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTc   >  2:3052858/1‑93 (MQ=255)

                                                                                       aGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg  <  2:6362230/93‑1 (MQ=255)

                                                                                       aGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCg  <  1:1180024/93‑1 (MQ=255)

GAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCG  >  NC_000913/4227624‑4227803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACA  >  NC_000913/4227621‑4227807

GCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCG                                                                                               >  SRR4302547.2827532/1‑94 (MQ=60)

  TGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGT                                                                                             <  SRR4302547.953784/94‑1 (MQ=60)

  TGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGT                                                                                             >  SRR4302547.2896806/1‑94 (MQ=60)

  TGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGT                                                                                             >  SRR4302547.861764/1‑94 (MQ=60)

  TGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGT                                                                                             >  SRR4302547.870875/1‑94 (MQ=60)

    AGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTC                                                                                           >  SRR4302547.4956/1‑94 (MQ=60)

    AGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTC                                                                                           >  SRR4302547.3164505/1‑94 (MQ=60)

     GCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCC                                                                                          <  SRR4302547.2480641/94‑1 (MQ=60)

       GGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCAT                                                                                        >  SRR4302547.6269438/1‑94 (MQ=60)

        GCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATT                                                                                       >  SRR4302547.859183/1‑94 (MQ=60)

        GCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGCCTTGACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATT                                                                                       <  SRR4302547.605568/94‑1 (MQ=60)

         CGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTA                                                                                      <  SRR4302547.276099/94‑1 (MQ=60)

         CGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTA                                                                                      <  SRR4302547.5590499/94‑1 (MQ=60)

          GACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTAC                                                                                     >  SRR4302547.320909/1‑94 (MQ=60)

          GACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTAC                                                                                     <  SRR4302547.540010/94‑1 (MQ=60)

           ACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACT                                                                                    >  SRR4302547.670284/1‑94 (MQ=60)

           ACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACT                                                                                    <  SRR4302547.6411012/94‑1 (MQ=60)

           ACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACT                                                                                    <  SRR4302547.1355033/94‑1 (MQ=60)

            CCCTGCAACTGTTCAGCAATTAGCGGCAGGGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGGTACTCAATAAAAGCGGGTCCATTTCTT                                                                                   >  SRR4302547.6724617/1‑94 (MQ=60)

             CCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTC                                                                                  <  SRR4302547.936901/94‑1 (MQ=60)

             CCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTC                                                                                  >  SRR4302547.937096/1‑94 (MQ=60)

              CTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCT                                                                                 <  SRR4302547.4921735/94‑1 (MQ=60)

              CTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCT                                                                                 >  SRR4302547.5967/1‑94 (MQ=60)

              CTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCT                                                                                 >  SRR4302547.4959060/1‑94 (MQ=60)

              CTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCT                                                                                 <  SRR4302547.71533/94‑1 (MQ=60)

              CTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCT                                                                                 <  SRR4302547.931056/94‑1 (MQ=60)

               TGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTC                                                                                >  SRR4302547.1139700/1‑94 (MQ=60)

               TGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTC                                                                                <  SRR4302547.2762151/94‑1 (MQ=60)

                GCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCC                                                                               >  SRR4302547.5807809/1‑94 (MQ=60)

                GCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCC                                                                               >  SRR4302547.394859/1‑94 (MQ=60)

                GCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCC                                                                               <  SRR4302547.1165278/94‑1 (MQ=60)

                 CAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCG                                                                              <  SRR4302547.5533295/94‑1 (MQ=60)

                 CAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCG                                                                              <  SRR4302547.4875319/94‑1 (MQ=60)

                 CAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCG                                                                              <  SRR4302547.1006285/94‑1 (MQ=60)

                  AACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGG                                                                             <  SRR4302547.4710877/94‑1 (MQ=60)

                   ACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGT                                                                            >  SRR4302547.993996/1‑94 (MQ=60)

                     TGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTA                                                                          >  SRR4302547.5729935/1‑94 (MQ=60)

                      GTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAG                                                                         <  SRR4302547.4854858/94‑1 (MQ=60)

                      GTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAG                                                                         <  SRR4302547.4580406/94‑1 (MQ=60)

                       TTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGT                                                                        <  SRR4302547.1693985/94‑1 (MQ=60)

                       TTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGT                                                                        <  SRR4302547.2438916/94‑1 (MQ=60)

                        TCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTA                                                                       <  SRR4302547.2155703/94‑1 (MQ=60)

                         CAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAA                                                                      <  SRR4302547.347785/94‑1 (MQ=60)

                         CAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAA                                                                      >  SRR4302547.2786687/1‑94 (MQ=60)

                          AGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAG                                                                     >  SRR4302547.2251169/1‑94 (MQ=60)

                           GCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGA                                                                    >  SRR4302547.6516245/1‑94 (MQ=60)

                             AATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAA                                                                  <  SRR4302547.5309429/94‑1 (MQ=60)

                             AATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAA                                                                  >  SRR4302547.4704000/1‑94 (MQ=60)

                              ATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAAT                                                                 >  SRR4302547.5682780/1‑94 (MQ=60)

                              ATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAAT                                                                 <  SRR4302547.4704885/94‑1 (MQ=60)

                              ATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAAT                                                                 <  SRR4302547.4303021/94‑1 (MQ=60)

                                TAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCA                                                               <  SRR4302547.6507190/94‑1 (MQ=60)

                                TAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCA                                                               <  SRR4302547.4616938/94‑1 (MQ=60)

                                TAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCA                                                               <  SRR4302547.6298830/94‑1 (MQ=60)

                                 AGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCAC                                                              <  SRR4302547.1708252/94‑1 (MQ=60)

                                  GCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACG                                                             <  SRR4302547.1279608/94‑1 (MQ=60)

                                  GCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACG                                                             <  SRR4302547.2278072/94‑1 (MQ=60)

                                  GCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACG                                                             <  SRR4302547.2563306/94‑1 (MQ=60)

                                  GCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACG                                                             <  SRR4302547.871825/94‑1 (MQ=60)

                                  GCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACG                                                             <  SRR4302547.6377340/94‑1 (MQ=60)

                                    GGCAGTGCAGGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGG                                                           <  SRR4302547.236937/94‑1 (MQ=60)

                                      GAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAG                                                         <  SRR4302547.6047924/94‑1 (MQ=60)

                                      CAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAG                                                         <  SRR4302547.3666261/94‑1 (MQ=60)

                                      CAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAG                                                         <  SRR4302547.1869249/94‑1 (MQ=60)

                                       AGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGG                                                        >  SRR4302547.6569998/1‑94 (MQ=60)

                                        GTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGC                                                       >  SRR4302547.4945067/1‑94 (MQ=60)

                                         TGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCA                                                      <  SRR4302547.1803402/94‑1 (MQ=60)

                                          GCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCAT                                                     <  SRR4302547.6423052/94‑1 (MQ=60)

                                           CATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATG                                                    <  SRR4302547.4874296/94‑1 (MQ=60)

                                              GTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTA                                                 >  SRR4302547.256961/1‑94 (MQ=60)

                                               TTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTAC                                                >  SRR4302547.5354073/1‑94 (MQ=60)

                                               TTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTAC                                                >  SRR4302547.2609201/1‑94 (MQ=60)

                                                 CCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCA                                              >  SRR4302547.4997349/1‑94 (MQ=60)

                                                 CCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCA                                              >  SRR4302547.6311464/1‑94 (MQ=60)

                                                 CCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCA                                              >  SRR4302547.358289/1‑94 (MQ=60)

                                                  CAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCAC                                             >  SRR4302547.1680271/1‑94 (MQ=60)

                                                   AGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACT                                            <  SRR4302547.1914938/94‑1 (MQ=60)

                                                   AGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACT                                            >  SRR4302547.799079/1‑94 (MQ=60)

                                                   AGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACT                                            >  SRR4302547.1068810/1‑94 (MQ=60)

                                                   AGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACT                                            <  SRR4302547.2596245/94‑1 (MQ=60)

                                                    GCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGGTAGTAAGAAATCACGGGAGGCATGCTACCACTG                                           >  SRR4302547.1552497/1‑94 (MQ=60)

                                                     CCAGGCTTTACCCGGCAGCGTCGAGTGACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGC                                          <  SRR4302547.3452294/94‑1 (MQ=60)

                                                      CAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCG                                         <  SRR4302547.628256/94‑1 (MQ=60)

                                                       AGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGA                                        <  SRR4302547.2623393/94‑1 (MQ=60)

                                                       AGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGGTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGA                                        >  SRR4302547.4051402/1‑94 (MQ=60)

                                                         GCTTTAGCCGCCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAAT                                      <  SRR4302547.5228661/94‑1 (MQ=60)

                                                           TTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTT                                    <  SRR4302547.3304936/94‑1 (MQ=60)

                                                            TTACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTA                                   <  SRR4302547.1963789/94‑1 (MQ=60)

                                                              ACCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAC                                 <  SRR4302547.2443303/94‑1 (MQ=60)

                                                               CCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACC                                >  SRR4302547.5251525/1‑94 (MQ=60)

                                                               CCCGGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACC                                <  SRR4302547.972006/94‑1 (MQ=60)

                                                                 CGGCAGCGTCGAGTTACTCAATAAAAGCGGGTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCAC                              >  SRR4302547.4348160/1‑94 (MQ=60)

                                                                  GGCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACT                             >  SRR4302547.73876/1‑94 (MQ=60)

                                                                  GGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACT                             >  SRR4302547.834378/1‑94 (MQ=60)

                                                                   GCAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTT                            <  SRR4302547.1761607/94‑1 (MQ=60)

                                                                    CAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTTCTTCTCCGTTTAGTAAGAAATCACGGGAGGCATGCTTCCCCTGCGAATTTAACCACTTA                           <  SRR4302547.6660236/94‑1 (MQ=60)

                                                                    CAGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTA                           >  SRR4302547.2316771/1‑94 (MQ=60)

                                                                     AGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTAC                          <  SRR4302547.6630157/94‑1 (MQ=60)

                                                                     AGCGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTAC                          >  SRR4302547.1388958/1‑94 (MQ=60)

                                                                      GCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACT                         <  SRR4302547.6032279/94‑1 (MQ=60)

                                                                       CGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAAGAACTTACTG                        <  SRR4302547.6675091/94‑1 (MQ=60)

                                                                       CGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTG                        <  SRR4302547.4786346/94‑1 (MQ=60)

                                                                       CGTCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTG                        <  SRR4302547.1236102/94‑1 (MQ=60)

                                                                        GTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGA                       >  SRR4302547.2246494/1‑94 (MQ=60)

                                                                         TCGAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGAC                      >  SRR4302547.3197536/1‑94 (MQ=60)

                                                                           GAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAT                    <  SRR4302547.4799201/94‑1 (MQ=60)

                                                                           GAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAT                    >  SRR4302547.894285/1‑94 (MQ=60)

                                                                           GAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAT                    >  SRR4302547.1685479/1‑94 (MQ=60)

                                                                           GAGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACAT                    <  SRR4302547.4322816/94‑1 (MQ=60)

                                                                            AGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATG                   >  SRR4302547.5769419/1‑94 (MQ=60)

                                                                            AGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATG                   <  SRR4302547.301757/94‑1 (MQ=60)

                                                                            AGTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATG                   <  SRR4302547.1132152/94‑1 (MQ=60)

                                                                            AGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATG                   <  SRR4302547.286103/94‑1 (MQ=60)

                                                                             GTTACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGG                  >  SRR4302547.204803/1‑94 (MQ=60)

                                                                               TACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCT                >  SRR4302547.3980034/1‑94 (MQ=60)

                                                                               TACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCT                <  SRR4302547.5954161/94‑1 (MQ=60)

                                                                               TACTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCC                >  SRR4302547.2572088/1‑94 (MQ=60)

                                                                                ACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTC               <  SRR4302547.670320/94‑1 (MQ=60)

                                                                                 CTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCA              <  SRR4302547.723482/94‑1 (MQ=60)

                                                                                 CTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCA              <  SRR4302547.742623/94‑1 (MQ=60)

                                                                                 CTCAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCA              <  SRR4302547.1835011/94‑1 (MQ=60)

                                                                                   CAATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACG            >  SRR4302547.6124896/1‑94 (MQ=60)

                                                                                    AATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGA           >  SRR4302547.5432243/1‑94 (MQ=60)

                                                                                    AATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGA           >  SRR4302547.4739674/1‑94 (MQ=60)

                                                                                     ATAAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGAT          <  SRR4302547.828788/94‑1 (MQ=60)

                                                                                       AAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGT        >  SRR4302547.771/1‑94 (MQ=60)

                                                                                       AAAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGT        >  SRR4302547.1511246/1‑94 (MQ=60)

                                                                                        AAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTC       >  SRR4302547.2400296/1‑94 (MQ=60)

                                                                                        AAAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTC       <  SRR4302547.5316125/94‑1 (MQ=60)

                                                                                         AAGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCG      <  SRR4302547.2918541/94‑1 (MQ=60)

                                                                                          AGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGC     <  SRR4302547.1203859/94‑1 (MQ=60)

                                                                                          AGCGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGC     <  SRR4302547.1191012/94‑1 (MQ=60)

                                                                                            CGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCAC   >  SRR4302547.6256896/1‑94 (MQ=60)

                                                                                            CGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCAC   >  SRR4302547.3963632/1‑94 (MQ=60)

                                                                                            CGGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCAC   >  SRR4302547.3357423/1‑94 (MQ=60)

                                                                                             GGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACA  >  SRR4302547.938485/1‑94 (MQ=60)

GCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAGCGGCAGTGCATGTTCCAGCCAGGCTTTACCCGGCAGCGTCGAGTTACTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACA  >  NC_000913/4227621‑4227807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`