Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,229,642 A→G L391L (TTG→CTG)  lysC ← aspartokinase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,229,6420AG99.6% 808.7 / ‑4.5 226L391L (TTG→CTG) lysCaspartokinase III
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (130/95);  total (131/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

Only 100 of 239 total aligned reads displayed.

CATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAG  >  NC_000913/4229552‑4229731
                                                                                          |                                                                                         
cATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGcg                                                                                         >  2:29490/1‑93 (MQ=255)
cATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGcg                                                                                         >  1:3100333/1‑93 (MQ=255)
cATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGcg                                                                                         >  1:1021263/1‑93 (MQ=255)
cATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGcg                                                                                         >  2:684962/1‑93 (MQ=255)
 aTAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAgcgc                                                                                        <  2:4684507/93‑1 (MQ=255)
 aTAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAgcgc                                                                                        <  1:2750286/93‑1 (MQ=255)
  tAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGTCAATCAGcgcg                                                                                       >  2:6076722/1‑93 (MQ=255)
      aaaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCa                                                                                   >  2:6009382/1‑93 (MQ=255)
      aaaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCa                                                                                   <  2:5510745/93‑1 (MQ=255)
      aaaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCa                                                                                   <  1:1361082/93‑1 (MQ=255)
       aaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAg                                                                                  >  2:509296/1‑93 (MQ=255)
       aaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAg                                                                                  >  2:4502913/1‑93 (MQ=255)
       aaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAg                                                                                  >  1:2693045/1‑93 (MQ=255)
       aaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAg                                                                                  >  1:5676147/1‑93 (MQ=255)
         tCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAgcg                                                                                >  2:2620393/1‑93 (MQ=255)
            tGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCa                                                                             >  1:1462994/1‑93 (MQ=255)
            tGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCa                                                                             >  1:5020338/1‑93 (MQ=255)
            tGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCa                                                                             <  1:4118438/93‑1 (MQ=255)
            tGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCa                                                                             <  2:1240461/93‑1 (MQ=255)
               gAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGAc                                                                          >  2:4997451/1‑93 (MQ=255)
                  tGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACctt                                                                       <  2:38552/93‑1 (MQ=255)
                   gTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACcttc                                                                      <  1:2053399/93‑1 (MQ=255)
                      gAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCttcttc                                                                   <  1:4328146/93‑1 (MQ=255)
                      gAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCttcttc                                                                   <  2:4651592/93‑1 (MQ=255)
                       aaCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtcc                                                                  <  1:3146458/93‑1 (MQ=255)
                        aCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtcca                                                                 >  1:4396169/1‑93 (MQ=255)
                        aCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtcca                                                                 <  2:203118/93‑1 (MQ=255)
                        aCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtcca                                                                 >  2:3700269/1‑93 (MQ=255)
                          ggTTCCAGTACGCCGAGTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtccacc                                                               >  1:5856616/1‑93 (MQ=255)
                           gTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtccacct                                                              >  2:3142223/1‑93 (MQ=255)
                           gTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtccacct                                                              >  1:6076841/1‑93 (MQ=255)
                             tCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtccacctcc                                                            >  1:4943657/1‑93 (MQ=255)
                              ccAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTtccacctcca                                                           >  1:1165652/1‑93 (MQ=255)
                                  tACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCg                                                       >  2:1093759/1‑93 (MQ=255)
                                      ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGacac                                                   <  2:5958799/93‑1 (MQ=255)
                                      ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGacac                                                   <  1:5101258/93‑1 (MQ=255)
                                       cGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCCCCTCCACCCGAcaca                                                  <  2:845186/93‑1 (MQ=255)
                                        gagtACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                 <  1:4608722/90‑1 (MQ=255)
                                        gAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                 >  2:1722315/1‑93 (MQ=255)
                                        gAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                 <  2:5654057/93‑1 (MQ=255)
                                         aaTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCGCCTCCACCCGACACAGt                                                <  1:3375747/93‑1 (MQ=255)
                                         aaTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGt                                                <  1:231986/93‑1 (MQ=255)
                                         aaTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGt                                                <  1:3068679/93‑1 (MQ=255)
                                          aTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTg                                               >  1:1109471/1‑93 (MQ=255)
                                           tACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGc                                              >  2:2308377/1‑93 (MQ=255)
                                           tACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGc                                              >  1:1859352/1‑93 (MQ=255)
                                            tccTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCg                                             <  1:4912941/92‑1 (MQ=255)
                                            aCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCg                                             >  1:618085/1‑93 (MQ=255)
                                            aCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCg                                             >  1:3899927/1‑93 (MQ=255)
                                             ccTCTTTTCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCgg                                            <  2:2256138/93‑1 (MQ=255)
                                               tctTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGaa                                          >  2:1778891/1‑93 (MQ=255)
                                               tctTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGaa                                          >  2:2555749/1‑93 (MQ=255)
                                                ctTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGaaa                                         <  2:4056373/93‑1 (MQ=255)
                                                 tttGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAg                                        <  2:3228726/93‑1 (MQ=255)
                                                  ttGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGc                                       <  1:4613034/93‑1 (MQ=255)
                                                    gCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTc                                     <  2:260631/93‑1 (MQ=255)
                                                    gCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTc                                     <  2:1497970/93‑1 (MQ=255)
                                                     ccAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTcc                                    >  1:5160973/1‑93 (MQ=255)
                                                     ccAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTcc                                    >  1:2754620/1‑93 (MQ=255)
                                                     ccAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTcc                                    <  1:3014144/93‑1 (MQ=255)
                                                      cAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCa                                   >  1:600400/1‑93 (MQ=255)
                                                        aCGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATc                                 <  2:1276327/93‑1 (MQ=255)
                                                         cgccgcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATca                                <  2:3404360/93‑1 (MQ=255)
                                                           ccgcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagc                              <  2:1359737/93‑1 (MQ=255)
                                                           ccgcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagc                              >  2:1497755/1‑93 (MQ=255)
                                                            cgcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagca                             >  2:4420643/1‑93 (MQ=255)
                                                            cgcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagca                             >  2:5291364/1‑93 (MQ=255)
                                                            cgcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagca                             <  1:932248/93‑1 (MQ=255)
                                                             gcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagcag                            >  1:829053/1‑93 (MQ=255)
                                                             gcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagcag                            <  1:3950829/93‑1 (MQ=255)
                                                             gcAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagcag                            <  1:4533385/93‑1 (MQ=255)
                                                              cAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCaga                           >  1:758648/1‑93 (MQ=255)
                                                                ggCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAgaga                         >  1:3115460/1‑93 (MQ=255)
                                                                ggCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCAACAGCAgaga                         <  1:3600261/93‑1 (MQ=255)
                                                                ggCTTTTGACAGGGCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAgaga                         >  1:5551957/1‑93 (MQ=255)
                                                                   ttttGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTg                      >  2:1654411/1‑93 (MQ=255)
                                                                   ttttGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTg                      >  1:5591919/1‑93 (MQ=255)
                                                                    tttGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGc                     >  2:3909397/1‑93 (MQ=255)
                                                                    tgtGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGc                     <  1:172398/91‑1 (MQ=255)
                                                                      tGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGt                   <  2:285968/93‑1 (MQ=255)
                                                                       gACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTc                  <  2:4850875/93‑1 (MQ=255)
                                                                       gACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTc                  <  2:3117284/93‑1 (MQ=255)
                                                                        aCAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCa                 <  2:1901087/93‑1 (MQ=255)
                                                                        aCAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCa                 >  2:3430692/1‑93 (MQ=255)
                                                                         cAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAg                >  1:2151645/1‑93 (MQ=255)
                                                                          agctCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGCCACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc               <  1:4717248/90‑1 (MQ=255)
                                                                          aGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc               >  2:5168974/1‑93 (MQ=255)
                                                                            gTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCaa             <  1:1950467/93‑1 (MQ=255)
                                                                               aTTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGt          >  1:5378352/1‑93 (MQ=255)
                                                                                ttGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTa         <  1:3720738/93‑1 (MQ=255)
                                                                                ttGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTa         >  1:5808631/1‑93 (MQ=255)
                                                                                ttGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTa         <  1:2462088/93‑1 (MQ=255)
                                                                                ttGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTa         >  1:1069022/1‑93 (MQ=255)
                                                                                 tGCCAATCAGCGCGACCAGCGCCAGCCCTTCTTCCACCTCCCCCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTAt        <  2:1696210/93‑1 (MQ=255)
                                                                                 tGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTAt        <  2:3782956/93‑1 (MQ=255)
                                                                                  gCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATc       <  2:4083877/93‑1 (MQ=255)
                                                                                  gCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATc       <  2:5722574/93‑1 (MQ=255)
                                                                                    cAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGc     >  1:4046453/1‑93 (MQ=255)
                                                                                      aTCAGCGCGACCAGCGCCAGACCGTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCa   <  1:5036160/93‑1 (MQ=255)
                                                                                       tCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAg  >  1:3576881/1‑93 (MQ=255)
                                                                                          |                                                                                         
CATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAG  >  NC_000913/4229552‑4229731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGA  >  NC_000913/4229549‑4229735
                                                                                             |                                                                                             
CGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAG                                                                                               <  SRR4302532.5624939/94‑1 (MQ=60)
CGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAG                                                                                               <  SRR4302532.4888742/94‑1 (MQ=60)
CGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAG                                                                                               >  SRR4302532.2198527/1‑94 (MQ=60)
CGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAG                                                                                               >  SRR4302532.1258672/1‑94 (MQ=60)
 GCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGGCATTGCCAATCAGC                                                                                              >  SRR4302532.474569/1‑94 (MQ=60)
  CCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCG                                                                                             >  SRR4302532.3203152/1‑94 (MQ=60)
  CCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCG                                                                                             >  SRR4302532.1055880/1‑94 (MQ=60)
  CCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCG                                                                                             >  SRR4302532.4388595/1‑94 (MQ=60)
  CCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCG                                                                                             >  SRR4302532.3026198/1‑94 (MQ=60)
   CATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGC                                                                                            >  SRR4302532.6032320/1‑94 (MQ=60)
   CATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGC                                                                                            >  SRR4302532.3010228/1‑94 (MQ=60)
   CATAACAAATCATGCGAATGTTGAACGGTTCCAGGACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGC                                                                                            >  SRR4302532.5105554/1‑94 (MQ=60)
    ATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCG                                                                                           <  SRR4302532.5181841/94‑1 (MQ=60)
    ATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCG                                                                                           <  SRR4302532.574902/94‑1 (MQ=60)
    ATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCG                                                                                           <  SRR4302532.2842658/94‑1 (MQ=60)
     TAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGA                                                                                          >  SRR4302532.5314550/1‑94 (MQ=60)
      AACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGAC                                                                                         <  SRR4302532.4175981/94‑1 (MQ=60)
       ACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACC                                                                                        <  SRR4302532.1074204/94‑1 (MQ=60)
       ACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACC                                                                                        >  SRR4302532.3241613/1‑94 (MQ=60)
         AAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAG                                                                                      >  SRR4302532.2783501/1‑94 (MQ=60)
         AAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAG                                                                                      >  SRR4302532.5874264/1‑94 (MQ=60)
         AAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAG                                                                                      <  SRR4302532.1406663/94‑1 (MQ=60)
            TCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGC                                                                                   >  SRR4302532.451687/1‑94 (MQ=60)
              ATGCGAATGTTGAACGGTTCCAGTACGCCGAATCCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCA                                                                                 <  SRR4302532.4986696/94‑1 (MQ=60)
              ATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCA                                                                                 <  SRR4302532.5274399/94‑1 (MQ=60)
              ATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCA                                                                                 >  SRR4302532.1512294/1‑94 (MQ=60)
              ATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCA                                                                                 >  SRR4302532.5196798/1‑94 (MQ=60)
              ATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCA                                                                                 >  SRR4302532.5192516/1‑94 (MQ=60)
               TGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAG                                                                                <  SRR4302532.4256662/94‑1 (MQ=60)
                      GTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCT                                                                         <  SRR4302532.2121783/94‑1 (MQ=60)
                         GAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCC                                                                      <  SRR4302532.4577876/94‑1 (MQ=60)
                         GAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCC                                                                      <  SRR4302532.4577877/94‑1 (MQ=60)
                         GAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCC                                                                      <  SRR4302532.4474386/94‑1 (MQ=60)
                         GAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCC                                                                      >  SRR4302532.4844643/1‑94 (MQ=60)
                          AACGTTTCCAGTACGCCGAATCCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCA                                                                     <  SRR4302532.2766248/94‑1 (MQ=60)
                          AACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCA                                                                     >  SRR4302532.4545736/1‑94 (MQ=60)
                          AACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCA                                                                     <  SRR4302532.3250865/94‑1 (MQ=60)
                            CGGTTCCAGTACGCCGAGTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACC                                                                   >  SRR4302532.6059702/1‑94 (MQ=60)
                             GGTTCCAGTACGCCGAATCCCTCTTTACCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGAGACCAGCGCCAGACCTTCTTCCACCT                                                                  <  SRR4302532.5664901/94‑1 (MQ=60)
                             GGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCT                                                                  >  SRR4302532.2019973/1‑94 (MQ=60)
                             GGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCT                                                                  >  SRR4302532.6286689/1‑94 (MQ=60)
                               TTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCC                                                                >  SRR4302532.5113340/1‑94 (MQ=60)
                                TCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCA                                                               >  SRR4302532.356522/1‑94 (MQ=60)
                                TCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCA                                                               >  SRR4302532.1204580/1‑94 (MQ=60)
                                TCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCA                                                               >  SRR4302532.2407309/1‑94 (MQ=60)
                                 CCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCAC                                                              >  SRR4302532.200960/1‑94 (MQ=60)
                                  tcctgctgaacgctcttccgatcttgccgatCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACC                                                             >  SRR4302532.4680826/32‑94 (MQ=60)
                                   AGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCC                                                            >  SRR4302532.665563/1‑94 (MQ=60)
                                        GCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACAC                                                       >  SRR4302532.5241293/1‑94 (MQ=60)
                                         CCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACA                                                      >  SRR4302532.175647/1‑94 (MQ=60)
                                         CCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACA                                                      <  SRR4302532.5276642/94‑1 (MQ=60)
                                         CCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACA                                                      >  SRR4302532.11681/1‑94 (MQ=60)
                                          CGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAG                                                     >  SRR4302532.3733202/1‑94 (MQ=60)
                                           GAGTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGT                                                    <  SRR4302532.4765861/94‑1 (MQ=60)
                                            AATCCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTG                                                   <  SRR4302532.4769498/94‑1 (MQ=60)
                                            AATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCGCCTCCACCCGACACAGTG                                                   <  SRR4302532.3488644/94‑1 (MQ=60)
                                            AATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTG                                                   >  SRR4302532.4222503/1‑94 (MQ=60)
                                            AATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTG                                                   >  SRR4302532.5099442/1‑94 (MQ=60)
                                            AATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTG                                                   <  SRR4302532.5110114/94‑1 (MQ=60)
                                            AATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTG                                                   >  SRR4302532.1146567/1‑94 (MQ=60)
                                            AATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTG                                                   <  SRR4302532.3170076/94‑1 (MQ=60)
                                            AATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTG                                                   <  SRR4302532.239909/94‑1 (MQ=60)
                                             ATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGC                                                  >  SRR4302532.1920988/1‑94 (MQ=60)
                                              TACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCG                                                 >  SRR4302532.4031452/1‑94 (MQ=60)
                                              TACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCG                                                 >  SRR4302532.639623/1‑94 (MQ=60)
                                              TACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCG                                                 >  SRR4302532.1649517/1‑94 (MQ=60)
                                              TACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCG                                                 <  SRR4302532.891355/94‑1 (MQ=60)
                                               TCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGG                                                <  SRR4302532.5081738/94‑1 (MQ=60)
                                                  TCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAA                                             <  SRR4302532.4078371/94‑1 (MQ=60)
                                                   CTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAG                                            >  SRR4302532.3859104/1‑94 (MQ=60)
                                                   CTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAG                                            >  SRR4302532.6280660/1‑94 (MQ=60)
                                                     TTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCT                                          >  SRR4302532.4728061/1‑94 (MQ=60)
                                                     TTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCT                                          <  SRR4302532.4770334/94‑1 (MQ=60)
                                                      TGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTC                                         >  SRR4302532.3170872/1‑94 (MQ=60)
                                                       GCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCC                                        <  SRR4302532.4787288/94‑1 (MQ=60)
                                                       GCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCC                                        <  SRR4302532.903975/94‑1 (MQ=60)
                                                       GCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCC                                        >  SRR4302532.5338589/1‑94 (MQ=60)
                                                       GCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCC                                        >  SRR4302532.2847132/1‑94 (MQ=60)
                                                        CCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCA                                       <  SRR4302532.3113626/94‑1 (MQ=60)
                                                        CCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCA                                       >  SRR4302532.621361/1‑94 (MQ=60)
                                                        CCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCA                                       <  SRR4302532.112613/94‑1 (MQ=60)
                                                         CAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCAT                                      >  SRR4302532.2173649/1‑94 (MQ=60)
                                                          AACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATC                                     >  SRR4302532.6191573/1‑94 (MQ=60)
                                                           ACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCA                                    <  SRR4302532.4052454/94‑1 (MQ=60)
                                                              CCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCA                                 >  SRR4302532.3205630/1‑94 (MQ=60)
                                                              CCGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCA                                 <  SRR4302532.63133/94‑1 (MQ=60)
                                                               CGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAG                                <  SRR4302532.964194/94‑1 (MQ=60)
                                                               CGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAG                                >  SRR4302532.857425/1‑94 (MQ=60)
                                                               CGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAG                                >  SRR4302532.4262009/1‑94 (MQ=60)
                                                               CGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAG                                >  SRR4302532.1842716/1‑94 (MQ=60)
                                                               CGCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAG                                <  SRR4302532.2602228/94‑1 (MQ=60)
                                                                GCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGA                               <  SRR4302532.4688116/94‑1 (MQ=60)
                                                                GCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGA                               >  SRR4302532.784701/1‑94 (MQ=60)
                                                                GCAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGA                               <  SRR4302532.4083974/94‑1 (MQ=60)
                                                                 CAGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAG                              <  SRR4302532.2830132/94‑1 (MQ=60)
                                                                  AGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGA                             >  SRR4302532.5797160/1‑94 (MQ=60)
                                                                  AGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGA                             >  SRR4302532.3708067/1‑94 (MQ=60)
                                                                  AGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGA                             >  SRR4302532.3322010/1‑94 (MQ=60)
                                                                  AGGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGA                             >  SRR4302532.3218838/1‑94 (MQ=60)
                                                                  AGGCTTTTGACAGGGCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGA                             >  SRR4302532.5744682/1‑94 (MQ=60)
                                                                   GGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGAT                            >  SRR4302532.814083/1‑94 (MQ=60)
                                                                   GGCTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCAACAGCAGAGAT                            <  SRR4302532.3720618/94‑1 (MQ=60)
                                                                     CTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTG                          >  SRR4302532.5786027/1‑94 (MQ=60)
                                                                     CTTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTG                          >  SRR4302532.3792515/1‑94 (MQ=60)
                                                                      TTTTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGC                         <  SRR4302532.5508951/94‑1 (MQ=60)
                                                                       TGTGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCG                        <  SRR4302532.178168/94‑1 (MQ=60)
                                                                         TGACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTC                      >  SRR4302532.2295760/1‑94 (MQ=60)
                                                                          GACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCA                     <  SRR4302532.1312334/94‑1 (MQ=60)
                                                                           ACAGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAG                    >  SRR4302532.2223541/1‑94 (MQ=60)
                                                                             AGGTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCA                  >  SRR4302532.5913108/1‑94 (MQ=60)
                                                                             AGCTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGCCACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCA                  <  SRR4302532.4878195/94‑1 (MQ=60)
                                                                              GGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGCAAGCTCCATCAGCAGAGATTGCGTCAGCAA                 >  SRR4302532.3125161/1‑94 (MQ=60)
                                                                               GTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAAC                <  SRR4302532.2262594/94‑1 (MQ=60)
                                                                               GTCATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAAC                <  SRR4302532.2015298/94‑1 (MQ=60)
                                                                                TCATTGCCAACCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACG               <  SRR4302532.586624/94‑1 (MQ=60)
                                                                                 CATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGT              >  SRR4302532.5564654/1‑94 (MQ=60)
                                                                                 CATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGT              >  SRR4302532.1930789/1‑94 (MQ=60)
                                                                                  ATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTA             >  SRR4302532.1104951/1‑94 (MQ=60)
                                                                                  ATTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTA             >  SRR4302532.6010438/1‑94 (MQ=60)
                                                                                   TTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTAT            <  SRR4302532.3253653/94‑1 (MQ=60)
                                                                                   TTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTAT            <  SRR4302532.2543993/94‑1 (MQ=60)
                                                                                   TTGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTAT            <  SRR4302532.3845656/94‑1 (MQ=60)
                                                                                    TGCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATC           <  SRR4302532.3175573/94‑1 (MQ=60)
                                                                                     GCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCG          <  SRR4302532.5575427/94‑1 (MQ=60)
                                                                                     GCCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCG          <  SRR4302532.6203929/94‑1 (MQ=60)
                                                                                      CCAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGC         >  SRR4302532.4182677/1‑94 (MQ=60)
                                                                                       CAATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGGAACGTATCGCC        >  SRR4302532.1438907/1‑94 (MQ=60)
                                                                                       CAATCAGAGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGCCACAGTaccgacaactcaagcacccaagattggggtggcgaggaatggcc        >  SRR4302532.4018455/1‑50 (MQ=51)
                                                                                        AATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCA       >  SRR4302532.1158836/1‑94 (MQ=60)
                                                                                         ATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAG      >  SRR4302532.2844109/1‑94 (MQ=60)
                                                                                         ATCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAG      >  SRR4302532.3696560/1‑94 (MQ=60)
                                                                                         ATCAGCGCGACCAGCGCCAGACCGTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAG      <  SRR4302532.5208884/94‑1 (MQ=60)
                                                                                          TCAGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGT     <  SRR4302532.6324460/94‑1 (MQ=60)
                                                                                            AGCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGG   >  SRR4302532.6238221/1‑94 (MQ=60)
                                                                                             GCGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGA  <  SRR4302532.4201659/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGA  >  NC_000913/4229549‑4229735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑