Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,232,040 A→G H235R (CAC→CGC)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,232,0400AG86.3% 684.6 / 84.6 262H235R (CAC→CGC) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (16/20);  new base G (98/128);  total (114/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 271 total aligned reads displayed.

GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGG  >  NC_000913/4231948‑4232129
                                                                                            |                                                                                         
   aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGt                                                                                        <  2:1866746/93‑1 (MQ=255)
   aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGt                                                                                        <  2:3925122/93‑1 (MQ=255)
    gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGtt                                                                                       >  2:2642745/1‑93 (MQ=255)
    gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGtt                                                                                       <  2:2770148/93‑1 (MQ=255)
     cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTg                                                                                      >  2:6042082/1‑93 (MQ=255)
      aTCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGc                                                                                     <  2:4245360/93‑1 (MQ=255)
      aTCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGGTGc                                                                                     >  2:5648905/1‑93 (MQ=255)
       tCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCa                                                                                    <  1:2136133/93‑1 (MQ=255)
       tCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGGTGCa                                                                                    >  2:468779/1‑93 (MQ=255)
            aaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAaca                                                                               >  1:6296019/1‑93 (MQ=255)
             aCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAacac                                                                              >  2:1896771/1‑93 (MQ=255)
             aCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAacac                                                                              >  2:4340350/1‑93 (MQ=255)
              ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACt                                                                             <  1:4146056/93‑1 (MQ=255)
              ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACt                                                                             >  2:4632393/1‑93 (MQ=255)
               cTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACtt                                                                            <  2:1335710/93‑1 (MQ=255)
                ttCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACttt                                                                           <  2:3448472/93‑1 (MQ=255)
                ttCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACttt                                                                           <  2:5838099/93‑1 (MQ=255)
                 tCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTg                                                                          >  1:1074517/1‑93 (MQ=255)
                     cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTgcggg                                                                      >  1:288067/1‑92 (MQ=255)
                     cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTgcggc                                                                      <  2:4764014/93‑1 (MQ=255)
                      acTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTgcggcg                                                                     >  2:4915652/1‑93 (MQ=255)
                       cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGgcgc                                                                    >  1:766278/1‑93 (MQ=255)
                       cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGgcgc                                                                    >  2:6441509/1‑93 (MQ=255)
                       cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGgcgc                                                                    <  1:6246029/93‑1 (MQ=255)
                        tCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCt                                                                   <  1:5841397/93‑1 (MQ=255)
                        tCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCt                                                                   <  2:3191868/93‑1 (MQ=255)
                         cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCtt                                                                  >  1:5654067/1‑93 (MQ=255)
                            gAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTcc                                                               >  2:1418789/1‑93 (MQ=255)
                             aaaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTcca                                                              <  1:3783961/93‑1 (MQ=255)
                              aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTccac                                                             <  1:3627434/93‑1 (MQ=255)
                              aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTccac                                                             <  1:4592384/93‑1 (MQ=255)
                               aCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGGAAAACGCGTTGCAAAACACTTTGCGGCGCTTTccacc                                                            >  2:338892/1‑93 (MQ=255)
                               aCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTccacc                                                            >  2:1591670/1‑93 (MQ=255)
                                    gACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGc                                                       <  1:5750267/93‑1 (MQ=255)
                                     aCCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGcc                                                      >  1:6363682/1‑93 (MQ=255)
                                       cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                    <  1:3004016/93‑1 (MQ=255)
                                        aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaaa                                                   >  2:2897158/1‑93 (MQ=255)
                                        aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaaa                                                   >  1:6627821/1‑93 (MQ=255)
                                          agCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGc                                                 <  1:650162/92‑1 (MQ=255)
                                             ccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                              <  1:4645880/93‑1 (MQ=255)
                                             ccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                              <  1:878594/93‑1 (MQ=255)
                                             ccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                              <  2:1675123/93‑1 (MQ=255)
                                              caaaGCGCGCCGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             <  1:5575179/90‑1 (MQ=255)
                                              cATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             >  1:5944378/1‑93 (MQ=255)
                                              cATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             <  2:696133/93‑1 (MQ=255)
                                              cATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                             <  2:5759327/93‑1 (MQ=255)
                                               aTAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTg                                            >  2:2104522/1‑93 (MQ=255)
                                                tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                           >  2:6570107/1‑93 (MQ=255)
                                                tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                           <  2:5447168/93‑1 (MQ=255)
                                                 aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                          <  2:5168590/93‑1 (MQ=255)
                                                 aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                          >  2:2509272/1‑93 (MQ=255)
                                                 aGCGCGCGTGACTGGTTCCTGAAAGCGACAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                          <  1:2686026/93‑1 (MQ=255)
                                                  gcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                         >  1:2224435/1‑93 (MQ=255)
                                                  gcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                         >  2:3494908/1‑93 (MQ=255)
                                                     cgctTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGt                                      <  2:3589215/93‑1 (MQ=255)
                                                     cgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGGCAAAGCCGTTGGCGAGt                                      >  2:3618987/1‑93 (MQ=255)
                                                     cgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGt                                      >  1:1393105/1‑93 (MQ=255)
                                                       cgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttt                                    <  1:1248822/93‑1 (MQ=255)
                                                        gTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                   <  1:1878718/93‑1 (MQ=255)
                                                        gTGACTGGTGCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                   <  1:3382180/93‑1 (MQ=255)
                                                         tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttttg                                  >  1:3267990/1‑91 (MQ=255)
                                                         tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTgg                                  >  2:4467509/1‑93 (MQ=255)
                                                          gACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                 <  1:450716/93‑1 (MQ=255)
                                                          gACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                 <  2:1274814/93‑1 (MQ=255)
                                                           aCTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                >  1:709214/1‑93 (MQ=255)
                                                            cTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                               <  1:417264/93‑1 (MQ=255)
                                                             tGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAtt                              <  2:1736023/93‑1 (MQ=255)
                                                             tGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAtt                              >  2:5432763/1‑93 (MQ=255)
                                                              ggTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTg                             <  1:4957082/93‑1 (MQ=255)
                                                              ggTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTg                             >  1:409055/1‑93 (MQ=255)
                                                               gTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            <  1:154883/93‑1 (MQ=255)
                                                               gTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            >  1:169488/1‑93 (MQ=255)
                                                               gTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            <  1:4030937/93‑1 (MQ=255)
                                                               gTTCCTGAAAGCGCCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                            <  2:3791838/93‑1 (MQ=255)
                                                                  ccTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                         <  2:1022260/93‑1 (MQ=255)
                                                                   cTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACt                        <  1:4547430/93‑1 (MQ=255)
                                                                    tGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTg                       >  1:2838187/1‑93 (MQ=255)
                                                                     gAAAGCGGCAGGGGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTGTTGCTACTGc                      >  1:3943908/1‑93 (MQ=255)
                                                                      aaaGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGcc                     <  2:6216393/93‑1 (MQ=255)
                                                                           ggCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACAt                >  2:6483282/1‑93 (MQ=255)
                                                                            gCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATg               <  2:2032269/93‑1 (MQ=255)
                                                                             cAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGt              >  2:1933935/1‑93 (MQ=255)
                                                                             cAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGt              <  1:19474/93‑1 (MQ=255)
                                                                              aGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt             >  2:537770/1‑93 (MQ=255)
                                                                               ggTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc            <  1:856767/93‑1 (MQ=255)
                                                                               ggTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc            <  2:639226/93‑1 (MQ=255)
                                                                                gTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg           <  1:290690/93‑1 (MQ=255)
                                                                                gTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg           <  2:2404134/93‑1 (MQ=255)
                                                                                gTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg           >  2:5871976/1‑93 (MQ=255)
                                                                                 tgatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa          <  1:4120803/93‑1 (MQ=255)
                                                                                 tgatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa          <  2:4086513/93‑1 (MQ=255)
                                                                                 tgatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa          <  2:4837649/93‑1 (MQ=255)
                                                                                 tgatgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCCACATGTTCGa          >  1:5256477/1‑93 (MQ=255)
                                                                                  gatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg         >  1:2075742/1‑93 (MQ=255)
                                                                                  gatgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg         >  1:5047365/1‑93 (MQ=255)
                                                                                   atgaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGt        >  1:2356766/1‑93 (MQ=255)
                                                                                     gaAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTc      <  1:2451798/93‑1 (MQ=255)
                                                                                       aaaaCGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTg    >  1:3431063/1‑93 (MQ=255)
                                                                                        aaaCGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgg   <  1:4279226/93‑1 (MQ=255)
                                                                                         aaCGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTggg  <  2:3525312/93‑1 (MQ=255)
                                                                                            |                                                                                         
GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGG  >  NC_000913/4231948‑4232129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTG  >  NC_000913/4231947‑4232133
                                                                                             |                                                                                             
TGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACG                                                                                               <  SRR4302547.103059/94‑1 (MQ=60)
TGCTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACG                                                                                               <  SRR4302547.3050571/94‑1 (MQ=60)
 GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGC                                                                                              >  SRR4302547.4395157/1‑94 (MQ=60)
 GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGC                                                                                              <  SRR4302547.5269105/94‑1 (MQ=60)
 GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACAC                                                                                              <  SRR4302547.6426483/94‑1 (MQ=60)
  GTAGCATCTAAAGCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCG                                                                                             <  SRR4302547.4438639/94‑1 (MQ=60)
  GTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCG                                                                                             >  SRR4302547.4207069/1‑94 (MQ=60)
  GTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCG                                                                                             >  SRR4302547.5897311/1‑94 (MQ=60)
  GTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCG                                                                                             >  SRR4302547.74842/1‑94 (MQ=60)
   TAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGT                                                                                            <  SRR4302547.2211560/94‑1 (MQ=60)
    accacctacacccttcccccCTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGCCTCGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTT                                                                                           <  SRR4302547.2631946/74‑1 (MQ=60)
    AGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTT                                                                                           <  SRR4302547.6533415/94‑1 (MQ=60)
    AGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTT                                                                                           <  SRR4302547.1411680/94‑1 (MQ=60)
     GCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTG                                                                                          <  SRR4302547.297071/94‑1 (MQ=60)
     GCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTG                                                                                          <  SRR4302547.2962366/94‑1 (MQ=60)
      CATCTAAACCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGC                                                                                         <  SRR4302547.3052073/94‑1 (MQ=60)
      CATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGC                                                                                         >  SRR4302547.783101/1‑94 (MQ=60)
      CATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGC                                                                                         >  SRR4302547.4185344/1‑94 (MQ=60)
       ATGTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCA                                                                                        <  SRR4302547.2134648/94‑1 (MQ=60)
        TCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAA                                                                                       <  SRR4302547.2178683/94‑1 (MQ=60)
         CTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAA                                                                                      >  SRR4302547.3144430/1‑94 (MQ=60)
          TAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAA                                                                                     >  SRR4302547.4934400/1‑94 (MQ=60)
          TAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAA                                                                                     >  SRR4302547.5953465/1‑94 (MQ=60)
            AAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACA                                                                                   >  SRR4302547.6426896/1‑94 (MQ=60)
            AAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACC                                                                                   >  SRR4302547.4522036/1‑94 (MQ=60)
             AACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACAC                                                                                  >  SRR4302547.4966521/1‑94 (MQ=60)
               CCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTT                                                                                <  SRR4302547.4230456/94‑1 (MQ=60)
                 TTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTG                                                                              >  SRR4302547.381128/1‑94 (MQ=60)
                 TTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTG                                                                              >  SRR4302547.1095956/1‑94 (MQ=60)
                 TTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTG                                                                              <  SRR4302547.1408396/94‑1 (MQ=60)
                  TCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGC                                                                             <  SRR4302547.6778229/94‑1 (MQ=60)
                   CACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCG                                                                            >  SRR4302547.5982236/1‑94 (MQ=60)
                   CACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGAGGCAGGTGATGAAAAACGCGCTGCAAAACACTTTGCG                                                                            >  SRR4302547.880867/1‑94 (MQ=60)
                    ACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGG                                                                           >  SRR4302547.1968088/1‑94 (MQ=60)
                     CCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGG                                                                          >  SRR4302547.293416/1‑94 (MQ=60)
                      CACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCG                                                                         >  SRR4302547.6573308/1‑94 (MQ=60)
                      CACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCG                                                                         <  SRR4302547.1844212/94‑1 (MQ=60)
                       ACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGC                                                                        >  SRR4302547.780744/1‑94 (MQ=60)
                       ACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGC                                                                        >  SRR4302547.2191597/1‑94 (MQ=60)
                        CTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCT                                                                       <  SRR4302547.6375777/94‑1 (MQ=60)
                         TCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTT                                                                      <  SRR4302547.5962255/94‑1 (MQ=60)
                         TCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTT                                                                      >  SRR4302547.5770794/1‑94 (MQ=60)
                          CAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTT                                                                     >  SRR4302547.2135969/1‑94 (MQ=60)
                            GGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCC                                                                   <  SRR4302547.4636038/94‑1 (MQ=60)
                             GAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCA                                                                  >  SRR4302547.405916/1‑94 (MQ=60)
                              AAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCAC                                                                 <  SRR4302547.3861112/94‑1 (MQ=60)
                               AACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACC                                                                <  SRR4302547.3701418/94‑1 (MQ=60)
                               AACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACC                                                                >  SRR4302547.3358398/1‑94 (MQ=60)
                               AACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACC                                                                >  SRR4302547.4114602/1‑94 (MQ=60)
                               AACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACC                                                                <  SRR4302547.4687415/94‑1 (MQ=60)
                               AACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACC                                                                >  SRR4302547.5712970/1‑94 (MQ=60)
                               AACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACC                                                                >  SRR4302547.1489454/1‑94 (MQ=60)
                                   ATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATG                                                            <  SRR4302547.2304535/94‑1 (MQ=60)
                                   ATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATG                                                            >  SRR4302547.2443909/1‑94 (MQ=60)
                                   ATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATG                                                            <  SRR4302547.5127176/94‑1 (MQ=60)
                                    TGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGC                                                           >  SRR4302547.1895737/1‑94 (MQ=60)
                                    TGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGC                                                           >  SRR4302547.3617172/1‑94 (MQ=60)
                                     GACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCC                                                          <  SRR4302547.5869129/94‑1 (MQ=60)
                                     GACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCC                                                          >  SRR4302547.6496440/1‑94 (MQ=60)
                                      ACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCA                                                         <  SRR4302547.4146468/94‑1 (MQ=60)
                                       CCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAA                                                        >  SRR4302547.146238/1‑94 (MQ=60)
                                       CCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGACAA                                                        >  SRR4302547.6459300/1‑94 (MQ=60)
                                        CAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAA                                                       <  SRR4302547.3063773/94‑1 (MQ=60)
                                        CAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAA                                                       <  SRR4302547.4254488/94‑1 (MQ=60)
                                        CAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAA                                                       >  SRR4302547.6765791/1‑94 (MQ=60)
                                         AACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCaaaac                                                      >  SRR4302547.4643603/1‑89 (MQ=60)
                                           CGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCC                                                    <  SRR4302547.3455714/94‑1 (MQ=60)
                                           AGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCC                                                    <  SRR4302547.662144/94‑1 (MQ=60)
                                            GCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCG                                                   >  SRR4302547.751716/1‑94 (MQ=60)
                                             CCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGT                                                  <  SRR4302547.3884902/94‑1 (MQ=60)
                                              CCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTT                                                 <  SRR4302547.895474/94‑1 (MQ=60)
                                              CCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTT                                                 <  SRR4302547.4742094/94‑1 (MQ=60)
                                              CCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTT                                                 >  SRR4302547.6067643/1‑94 (MQ=60)
                                               CATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTG                                                <  SRR4302547.420854/94‑1 (MQ=60)
                                               CAAAGCGCGCCGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTG                                                <  SRR4302547.5690135/94‑1 (MQ=60)
                                                ATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGG                                               <  SRR4302547.4636944/94‑1 (MQ=60)
                                                  AGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCG                                             >  SRR4302547.2269261/1‑94 (MQ=60)
                                                  AGCGCGCGTGACTGGTTCCTGAAAGCGACAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCG                                             <  SRR4302547.2739942/94‑1 (MQ=60)
                                                     GCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGT                                          >  SRR4302547.1420983/1‑94 (MQ=60)
                                                      CGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTT                                         <  SRR4302547.1585764/94‑1 (MQ=60)
                                                       GCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTT                                        <  SRR4302547.5396546/94‑1 (MQ=60)
                                                       GCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTT                                        >  SRR4302547.1920990/1‑94 (MQ=60)
                                                       GCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTT                                        <  SRR4302547.811085/94‑1 (MQ=60)
                                                       GCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTT                                        <  SRR4302547.4650342/94‑1 (MQ=60)
                                                        CGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTG                                       <  SRR4302547.1274029/94‑1 (MQ=60)
                                                        CGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTG                                       <  SRR4302547.3491686/94‑1 (MQ=60)
                                                         GTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTTG                                      >  SRR4302547.3333378/1‑94 (MQ=60)
                                                         GTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGG                                      <  SRR4302547.5711541/94‑1 (MQ=60)
                                                         GTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGG                                      <  SRR4302547.1915831/94‑1 (MQ=60)
                                                         GTGACTGGTGCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGG                                      <  SRR4302547.3450924/94‑1 (MQ=60)
                                                           GACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTA                                    <  SRR4302547.5414310/94‑1 (MQ=60)
                                                           GACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTA                                    <  SRR4302547.459077/94‑1 (MQ=60)
                                                           GACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTA                                    >  SRR4302547.722331/1‑94 (MQ=60)
                                                           GACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTA                                    >  SRR4302547.6359511/1‑94 (MQ=60)
                                                            ACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAT                                   <  SRR4302547.6721905/94‑1 (MQ=60)
                                                             ctgcgTCCTGACAGCGGCAGGTGATGAACACCGCGTGGCAAAGCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGTCGAGTTTGGTATT                                  <  SRR4302547.2201414/89‑1 (MQ=60)
                                                             CTGGTTCCTGAAAGCGGCAGGTGATGAAAATCGCGTTGCAAAACACTTTGCGGCACTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATT                                  <  SRR4302547.5924739/94‑1 (MQ=60)
                                                             CTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATT                                  <  SRR4302547.4498922/94‑1 (MQ=60)
                                                             CTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATT                                  <  SRR4302547.11814/94‑1 (MQ=60)
                                                             CTGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATT                                  <  SRR4302547.2300734/94‑1 (MQ=60)
                                                             CTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATT                                  <  SRR4302547.425000/94‑1 (MQ=60)
                                                              TGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTG                                 >  SRR4302547.4232013/1‑94 (MQ=60)
                                                              TGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTG                                 >  SRR4302547.416640/1‑94 (MQ=60)
                                                              TGGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTACAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTG                                 <  SRR4302547.6035608/94‑1 (MQ=60)
                                                               GGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGA                                >  SRR4302547.172592/1‑94 (MQ=60)
                                                               GGTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGA                                <  SRR4302547.5059676/94‑1 (MQ=60)
                                                                GTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAT                               <  SRR4302547.4112810/94‑1 (MQ=60)
                                                                GTTCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAT                               <  SRR4302547.157715/94‑1 (MQ=60)
                                                                  TCCTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGTGCAAGCCGTTGGCGAGTTTGGTTTTGATAC                             >  SRR4302547.4313396/1‑94 (MQ=60)
                                                                    CTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTG                           >  SRR4302547.642400/1‑94 (MQ=60)
                                                                    CTGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTG                           >  SRR4302547.2894915/1‑94 (MQ=60)
                                                                    CTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTG                           >  SRR4302547.2745523/1‑94 (MQ=60)
                                                                    CTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTG                           <  SRR4302547.4641477/94‑1 (MQ=60)
                                                                     TGAAAGCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGC                          <  SRR4302547.6782771/94‑1 (MQ=60)
                                                                     TGAAAGCGGCAGGGGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTGTTGCTACTGC                          >  SRR4302547.4024120/1‑94 (MQ=60)
                                                                          GCGGCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACA                     <  SRR4302547.4321683/94‑1 (MQ=60)
                                                                             GCAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGT                  <  SRR4302547.5097848/94‑1 (MQ=60)
                                                                              CAGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTT                 <  SRR4302547.19847/94‑1 (MQ=60)
                                                                               AGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTC                <  SRR4302547.5026076/94‑1 (MQ=60)
                                                                               AGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTC                >  SRR4302547.6337958/1‑94 (MQ=60)
                                                                               AGGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTC                <  SRR4302547.2945136/94‑1 (MQ=60)
                                                                               AGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTC                <  SRR4302547.5347720/94‑1 (MQ=60)
                                                                                GGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG               <  SRR4302547.471160/94‑1 (MQ=60)
                                                                                GGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG               <  SRR4302547.4044435/94‑1 (MQ=60)
                                                                                GGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG               <  SRR4302547.873139/94‑1 (MQ=60)
                                                                                GGTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG               <  SRR4302547.2690111/94‑1 (MQ=60)
                                                                                GGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG               <  SRR4302547.2083162/94‑1 (MQ=60)
                                                                                GGAGATGAAAAACGCGATGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCAGTTGGCGAGTTTGGTATTGATACTGCCAACATGCTCG               >  SRR4302547.2767476/1‑94 (MQ=60)
                                                                                CGTGATGGAAAACGCGTTGCAAAAGACTTTGCGGCGCTTTCCACCAATTCCACAGCCGATGGTGAGATTGGTATTGATACTGCCAACATGTTCG               >  SRR4302547.4521614/1‑94 (MQ=60)
                                                                                 GTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGA              <  SRR4302547.3512913/94‑1 (MQ=60)
                                                                                 GTGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGA              <  SRR4302547.296086/94‑1 (MQ=60)
                                                                                 GTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCCACATGTTCGA              >  SRR4302547.5364706/1‑94 (MQ=60)
                                                                                  TGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAG             <  SRR4302547.4204542/94‑1 (MQ=60)
                                                                                  TGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAG             <  SRR4302547.3294250/94‑1 (MQ=60)
                                                                                  TGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAG             >  SRR4302547.2117147/1‑94 (MQ=60)
                                                                                  TGATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAG             >  SRR4302547.5151575/1‑94 (MQ=60)
                                                                                   GATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGT            >  SRR4302547.2404526/1‑94 (MQ=60)
                                                                                    ATGAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTT           <  SRR4302547.386005/94‑1 (MQ=60)
                                                                                    ATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTT           >  SRR4302547.2915782/1‑94 (MQ=60)
                                                                                      GAAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCT         <  SRR4302547.2501339/94‑1 (MQ=60)
                                                                                       AAAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTG        >  SRR4302547.3500975/1‑94 (MQ=60)
                                                                                        AAAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGG       <  SRR4302547.5859446/94‑1 (MQ=60)
                                                                                         AAACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGG      <  SRR4302547.4367116/94‑1 (MQ=60)
                                                                                         AAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGG      >  SRR4302547.2716524/1‑94 (MQ=60)
                                                                                          AACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGG     >  SRR4302547.1454774/1‑94 (MQ=60)
                                                                                           ACGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGAC    >  SRR4302547.850529/1‑94 (MQ=60)
                                                                                            CGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACT   >  SRR4302547.3865545/1‑94 (MQ=60)
                                                                                            CGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACT   <  SRR4302547.1881802/94‑1 (MQ=60)
                                                                                            CGCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACT   >  SRR4302547.3952280/1‑94 (MQ=60)
                                                                                             GCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTG  >  SRR4302547.5841777/1‑94 (MQ=60)
                                                                                             GCGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTG  <  SRR4302547.3354174/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTG  >  NC_000913/4231947‑4232133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑