Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,254,178	A→G	intergenic (‑133/‑38)	plsB ← / → dgkA	glycerol‑3‑phosphate acyltransferase/diacylglycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,254,178	0	A	G	86.1%	745.4 / 98.8	280	intergenic (‑133/‑38)	plsB/dgkA	glycerol‑3‑phosphate acyltransferase/diacylglycerol kinase
Reads supporting (aligned to +/- strand): ref base A (27/12); new base G (110/131); total (137/143)
Fisher's exact test for biased strand distribution p-value = 8.98e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 284 total aligned reads displayed.

AAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCT  >  NC_000913/4254087‑4254270

aaGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTacac                                                                                             >  2:469924/1‑93 (MQ=255)

    aTAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGat                                                                                         <  1:6373872/93‑1 (MQ=255)

    aTAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGat                                                                                         >  1:1905734/1‑93 (MQ=255)

    aTAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGat                                                                                         <  1:3694739/93‑1 (MQ=255)

     tAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGata                                                                                        <  1:4794362/93‑1 (MQ=255)

     tAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGata                                                                                        <  1:329916/93‑1 (MQ=255)

     tAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGata                                                                                        >  2:5272199/1‑93 (MQ=255)

       gCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATAtt                                                                                      <  2:4977881/93‑1 (MQ=255)

            aaGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGc                                                                                 >  1:113380/1‑93 (MQ=255)

            aaGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGc                                                                                 <  1:5709897/93‑1 (MQ=255)

            aaGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGc                                                                                 >  2:3732290/1‑93 (MQ=255)

             aGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCt                                                                                >  1:743924/1‑93 (MQ=255)

              gTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTc                                                                               >  1:3664798/1‑93 (MQ=255)

              gTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTc                                                                               <  1:5173235/93‑1 (MQ=255)

              gTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTc                                                                               >  2:5649385/1‑93 (MQ=255)

                 aTGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATa                                                                            >  2:4901589/1‑93 (MQ=255)

                  tGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATaa                                                                           >  1:1783761/1‑93 (MQ=255)

                  tGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATaa                                                                           <  1:1994226/93‑1 (MQ=255)

                       aaTTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTaa                                                                      <  1:3489292/93‑1 (MQ=255)

                       aaTTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTaa                                                                      <  2:2265604/93‑1 (MQ=255)

                          tCTGTGGTATCCTCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTaaaaa                                                                   <  1:5563353/93‑1 (MQ=255)

                          tCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTaaaaa                                                                   <  1:2184125/93‑1 (MQ=255)

                          tCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTaaaaa                                                                   <  2:5152126/93‑1 (MQ=255)

                          tCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTaaaaa                                                                   >  2:3221679/1‑93 (MQ=255)

                            tgtgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAgg                                                                 <  1:558655/93‑1 (MQ=255)

                            tgtgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAgg                                                                 >  1:110127/1‑93 (MQ=255)

                            tgtgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAgg                                                                 <  1:2668342/93‑1 (MQ=255)

                            tgtgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAgg                                                                 >  2:5544004/1‑93 (MQ=255)

                            tgtgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAgg                                                                 <  2:6052131/93‑1 (MQ=255)

                            tgtgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAgg                                                                 >  1:4097412/1‑93 (MQ=255)

                              tgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGtt                                                               >  1:5932775/1‑93 (MQ=255)

                                 tATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCtt                                                            >  1:845943/1‑93 (MQ=255)

                                 tATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCtt                                                            <  2:3460153/93‑1 (MQ=255)

                                 tATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCtt                                                            >  1:5982401/1‑93 (MQ=255)

                                  aTCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCttt                                                           >  2:10342/1‑93 (MQ=255)

                                  aTCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCttt                                                           >  1:6293356/1‑93 (MQ=255)

                                  aTCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCttt                                                           >  1:3785930/1‑93 (MQ=255)

                                  aTCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCttt                                                           <  1:2796963/93‑1 (MQ=255)

                                  aTCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCttt                                                           <  2:6010994/93‑1 (MQ=255)

                                   tCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTc                                                          >  2:1778695/1‑93 (MQ=255)

                                   tCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTc                                                          >  2:2692637/1‑93 (MQ=255)

                                   tCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTc                                                          >  2:791127/1‑93 (MQ=255)

                                   tCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTc                                                          <  1:5874958/93‑1 (MQ=255)

                                   tCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTc                                                          >  2:2159190/1‑93 (MQ=255)

                                      gCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAAt                                                       <  1:2038152/93‑1 (MQ=255)

                                      gCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAAt                                                       <  1:3019243/93‑1 (MQ=255)

                                      gCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAAt                                                       >  2:2621770/1‑93 (MQ=255)

                                      gCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGGACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCaac                                                       >  2:5965653/1‑92 (MQ=255)

                                       cTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATg                                                      >  1:4878779/1‑93 (MQ=255)

                                        tCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATgg                                                     <  2:2506923/93‑1 (MQ=255)

                                        tCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATgg                                                     >  1:6007542/1‑93 (MQ=255)

                                            gTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCaa                                                 <  1:4215747/93‑1 (MQ=255)

                                              ttCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCaata                                               <  2:5386052/93‑1 (MQ=255)

                                              ttCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCaata                                               <  2:3849537/93‑1 (MQ=255)

                                               tCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCaataa                                              <  1:2407650/93‑1 (MQ=255)

                                                 gcgcgGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAataata                                            >  2:4538005/1‑93 (MQ=255)

                                                 gcgcgGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAataata                                            <  1:4259243/93‑1 (MQ=255)

                                                  cgcgGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATAc                                           <  2:5029399/93‑1 (MQ=255)

                                                  cgcgGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATAc                                           <  1:5462020/93‑1 (MQ=255)

                                                   gcggcgCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATAcc                                          <  2:66431/93‑1 (MQ=255)

                                                      gcgcTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACt                                       >  1:5285969/1‑93 (MQ=255)

                                                      gcgcTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACt                                       >  2:5010662/1‑93 (MQ=255)

                                                        gcTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTgg                                     >  1:1951860/1‑93 (MQ=255)

                                                         cTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGa                                    <  1:6423123/93‑1 (MQ=255)

                                                          tACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGAt                                   <  2:1909290/93‑1 (MQ=255)

                                                           aCGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGAtt                                  >  1:3988957/1‑93 (MQ=255)

                                                           aCGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGAtt                                  >  2:6187894/1‑93 (MQ=255)

                                                            cGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGATGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTc                                 >  2:3569650/1‑93 (MQ=255)

                                                             gCAACCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCa                                <  1:358485/93‑1 (MQ=255)

                                                               aaaCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCAcc                              <  1:780853/93‑1 (MQ=255)

                                                               aaaCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCAcc                              <  2:6416792/93‑1 (MQ=255)

                                                                 aCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCg                            <  1:2366701/93‑1 (MQ=255)

                                                                 aCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCg                            >  1:4840145/1‑93 (MQ=255)

                                                                 aCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCg                            >  1:4660557/1‑93 (MQ=255)

                                                                  cccGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGa                           >  1:4730863/1‑93 (MQ=255)

                                                                   ccGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGaa                          >  2:1664884/1‑93 (MQ=255)

                                                                   ccGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGaa                          <  1:3073556/93‑1 (MQ=255)

                                                                   ccGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGaa                          <  1:6191656/93‑1 (MQ=255)

                                                                      aaTCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTa                       <  2:2805068/93‑1 (MQ=255)

                                                                      aaTCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTa                       <  2:4158576/93‑1 (MQ=255)

                                                                      aaTCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTa                       <  2:4525021/93‑1 (MQ=255)

                                                                      aaTCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTa                       <  2:5813484/93‑1 (MQ=255)

                                                                       atcatcGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTAt                      >  2:3016691/1‑93 (MQ=255)

                                                                        tcatcGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTCCAATGGCCAATAATACCACTGGATTCACCCGAATTATc                     <  1:5639549/93‑1 (MQ=255)

                                                                          atcGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCaa                   <  2:2907299/93‑1 (MQ=255)

                                                                          atcGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCaa                   >  2:119382/1‑93 (MQ=255)

                                                                           tcGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCaaa                  >  2:4089654/1‑93 (MQ=255)

                                                                           tcGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCaaa                  >  1:5217319/1‑93 (MQ=255)

                                                                            cGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAg                 <  2:4219408/93‑1 (MQ=255)

                                                                            cGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAg                 <  2:1524114/93‑1 (MQ=255)

                                                                             ggATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAgc                >  2:443768/1‑93 (MQ=255)

                                                                             ggATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAgc                >  2:391651/1‑93 (MQ=255)

                                                                             ggATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAgc                <  2:315255/93‑1 (MQ=255)

                                                                              gATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAgct               <  1:2579304/93‑1 (MQ=255)

                                                                              gATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAgct               >  2:2863788/1‑93 (MQ=255)

                                                                              gATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAgct               >  1:946642/1‑93 (MQ=255)

                                                                                tttAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAgctgc             <  2:3664820/93‑1 (MQ=255)

                                                                                    aCGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGc         >  1:382622/1‑93 (MQ=255)

                                                                                      ggTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTa       >  1:1457421/1‑93 (MQ=255)

                                                                                       gTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTAt      <  1:4383159/93‑1 (MQ=255)

AAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCT  >  NC_000913/4254087‑4254270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTG  >  NC_000913/4254085‑4254271

GCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACG                                                                                               <  SRR4302547.389671/94‑1 (MQ=60)

GCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACG                                                                                               <  SRR4302547.5693687/94‑1 (MQ=60)

GCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACG                                                                                               <  SRR4302547.1811509/94‑1 (MQ=60)

  AAGGATAGCAAAAAGTCATAGGAAATTCTGTTGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCT                                                                                             <  SRR4302547.5974079/94‑1 (MQ=60)

   AGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTG                                                                                            <  SRR4302547.4829327/94‑1 (MQ=60)

    GGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGA                                                                                           >  SRR4302547.6124090/1‑94 (MQ=60)

     GATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGAT                                                                                          >  SRR4302547.1943446/1‑94 (MQ=60)

      ATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATA                                                                                         <  SRR4302547.3770161/94‑1 (MQ=60)

      ATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATA                                                                                         <  SRR4302547.6225261/94‑1 (MQ=60)

      ATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATA                                                                                         <  SRR4302547.6506856/94‑1 (MQ=60)

       TAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATAT                                                                                        <  SRR4302547.336058/94‑1 (MQ=60)

       TAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATAT                                                                                        <  SRR4302547.4893707/94‑1 (MQ=60)

        AGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATT                                                                                       >  SRR4302547.5522642/1‑94 (MQ=60)

         GCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTG                                                                                      <  SRR4302547.4431033/94‑1 (MQ=60)

          CAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGA                                                                                     >  SRR4302547.1792282/1‑94 (MQ=60)

          CAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGA                                                                                     <  SRR4302547.785187/94‑1 (MQ=60)

            GAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACG                                                                                   <  SRR4302547.2011421/94‑1 (MQ=60)

            GAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACG                                                                                   >  SRR4302547.892655/1‑94 (MQ=60)

            GAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACG                                                                                   <  SRR4302547.654112/94‑1 (MQ=60)

             AAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGC                                                                                  >  SRR4302547.115443/1‑94 (MQ=60)

             AAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGC                                                                                  >  SRR4302547.3619730/1‑94 (MQ=60)

              AAGTCATGGGAAATTCTGTGTTATCCGCTCATGTTTCGCGCGGCGCTACGCAAGCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCT                                                                                 <  SRR4302547.951958/94‑1 (MQ=60)

              AAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCT                                                                                 >  SRR4302547.757931/1‑94 (MQ=60)

              AAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCT                                                                                 <  SRR4302547.5827832/94‑1 (MQ=60)

               AGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTC                                                                                >  SRR4302547.3739558/1‑94 (MQ=60)

               AGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTC                                                                                <  SRR4302547.3082667/94‑1 (MQ=60)

                GTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCA                                                                               <  SRR4302547.5279732/94‑1 (MQ=60)

                   ATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAA                                                                            <  SRR4302547.4469832/94‑1 (MQ=60)

                   ATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAA                                                                            >  SRR4302547.2183942/1‑94 (MQ=60)

                   ATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAA                                                                            >  SRR4302547.1819093/1‑94 (MQ=60)

                   ATGGGAAATGCTGTGGTATCTGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAA                                                                            >  SRR4302547.6635356/1‑94 (MQ=60)

                    TGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAAT                                                                           <  SRR4302547.2033889/94‑1 (MQ=60)

                    TGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAAT                                                                           >  SRR4302547.4963012/1‑94 (MQ=60)

                     GGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATG                                                                          <  SRR4302547.3887604/94‑1 (MQ=60)

                      GGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGT                                                                         <  SRR4302547.2452087/94‑1 (MQ=60)

                       GAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTA                                                                        <  SRR4302547.632966/94‑1 (MQ=60)

                         AATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAA                                                                      <  SRR4302547.3560491/94‑1 (MQ=60)

                          ATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAA                                                                     >  SRR4302547.5609802/1‑94 (MQ=60)

                            TCTGTGGTATCCTCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAG                                                                   <  SRR4302547.5678092/94‑1 (MQ=60)

                            TCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAG                                                                   <  SRR4302547.2227894/94‑1 (MQ=60)

                            TCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAG                                                                   >  SRR4302547.6025045/1‑94 (MQ=60)

                             CTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGG                                                                  >  SRR4302547.5703210/1‑94 (MQ=60)

                             CTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGG                                                                  >  SRR4302547.112139/1‑94 (MQ=60)

                             CTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGG                                                                  >  SRR4302547.5237044/1‑94 (MQ=60)

                             CTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGG                                                                  >  SRR4302547.5913954/1‑94 (MQ=60)

                             CTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGG                                                                  >  SRR4302547.4180732/1‑94 (MQ=60)

                              TGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGT                                                                 <  SRR4302547.2721958/94‑1 (MQ=60)

                              TGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGT                                                                 <  SRR4302547.568715/94‑1 (MQ=60)

                               GTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTT                                                                <  SRR4302547.4868034/94‑1 (MQ=60)

                               GTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTT                                                                <  SRR4302547.6378663/94‑1 (MQ=60)

                               GTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTT                                                                >  SRR4302547.6055795/1‑94 (MQ=60)

                                TGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTC                                                               >  SRR4302547.3767699/1‑94 (MQ=60)

                                TGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTC                                                               >  SRR4302547.3700924/1‑94 (MQ=60)

                                TGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTC                                                               >  SRR4302547.415021/1‑94 (MQ=60)

                                  GTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTT                                                             >  SRR4302547.862095/1‑94 (MQ=60)

                                  GTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTT                                                             <  SRR4302547.2040779/94‑1 (MQ=60)

                                  GTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTT                                                             >  SRR4302547.6106502/1‑94 (MQ=60)

                                   TATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTT                                                            >  SRR4302547.6424179/1‑94 (MQ=60)

                                   TATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTT                                                            >  SRR4302547.3863122/1‑94 (MQ=60)

                                   TATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTT                                                            <  SRR4302547.5825798/94‑1 (MQ=60)

                                   TATCCGCTCATGTGTCGCGCGGCGCTACGCAACCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTT                                                            <  SRR4302547.5392874/94‑1 (MQ=60)

                                    ATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTC                                                           >  SRR4302547.5312622/1‑94 (MQ=60)

                                    ATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTC                                                           <  SRR4302547.2131377/94‑1 (MQ=60)

                                    ATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTC                                                           <  SRR4302547.2852954/94‑1 (MQ=60)

                                     TCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCA                                                          <  SRR4302547.5996577/94‑1 (MQ=60)

                                      CCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAA                                                         <  SRR4302547.6237985/94‑1 (MQ=60)

                                      CCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAA                                                         >  SRR4302547.4797510/1‑94 (MQ=60)

                                       CGCTCATGTTTGGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAAT                                                        >  SRR4302547.4251752/1‑94 (MQ=60)

                                       CGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAAT                                                        <  SRR4302547.491171/94‑1 (MQ=60)

                                       CGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATAATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGGTCTTTTAAT                                                        >  SRR4302547.1119337/1‑94 (MQ=60)

                                        GCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATG                                                       <  SRR4302547.3079282/94‑1 (MQ=60)

                                        GCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATG                                                       >  SRR4302547.4979865/1‑94 (MQ=60)

                                        GCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATG                                                       <  SRR4302547.2078796/94‑1 (MQ=60)

                                         CTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGG                                                      >  SRR4302547.267641/1‑94 (MQ=60)

                                         CTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGG                                                      >  SRR4302547.6132234/1‑94 (MQ=60)

                                          TCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGC                                                     <  SRR4302547.1352095/94‑1 (MQ=60)

                                            ATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCA                                                   <  SRR4302547.3629813/94‑1 (MQ=60)

                                            ATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCA                                                   >  SRR4302547.2696347/1‑94 (MQ=60)

                                             TGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAA                                                  >  SRR4302547.5682818/1‑94 (MQ=60)

                                              GTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAAT                                                 <  SRR4302547.4301804/94‑1 (MQ=60)

                                              GTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAAT                                                 <  SRR4302547.6058167/94‑1 (MQ=60)

                                              GTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAAT                                                 <  SRR4302547.1518055/94‑1 (MQ=60)

                                               TTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATA                                                <  SRR4302547.3288549/94‑1 (MQ=60)

                                               TTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATA                                                <  SRR4302547.3765837/94‑1 (MQ=60)

                                                 TCGCGCGTCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAAT                                              <  SRR4302547.979822/94‑1 (MQ=60)

                                                 TCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAAT                                              <  SRR4302547.2456397/94‑1 (MQ=60)

                                                  CGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATA                                             >  SRR4302547.3401666/1‑94 (MQ=60)

                                                  CGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATA                                             <  SRR4302547.6510909/94‑1 (MQ=60)

                                                   GCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATAC                                            <  SRR4302547.4346404/94‑1 (MQ=60)

                                                   GCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATAC                                            <  SRR4302547.5765748/94‑1 (MQ=60)

                                                   GCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATAC                                            >  SRR4302547.3317043/1‑94 (MQ=60)

                                                    CGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACC                                           <  SRR4302547.5574856/94‑1 (MQ=60)

                                                     GCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCA                                          >  SRR4302547.5159508/1‑94 (MQ=60)

                                                       GGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACT                                        <  SRR4302547.3398009/94‑1 (MQ=60)

                                                       GGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACT                                        >  SRR4302547.5395009/1‑94 (MQ=60)

                                                       GGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACT                                        >  SRR4302547.201844/1‑94 (MQ=60)

                                                        GCGCTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTG                                       >  SRR4302547.1963769/1‑94 (MQ=60)

                                                         CGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGG                                      >  SRR4302547.1990576/1‑94 (MQ=60)

                                                           CTACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGAT                                    >  SRR4302547.1142549/1‑94 (MQ=60)

                                                           CTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGAT                                    <  SRR4302547.6557025/94‑1 (MQ=60)

                                                            TACGCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATT                                   >  SRR4302547.4070066/1‑94 (MQ=60)

                                                               GCAACCCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCAC                                <  SRR4302547.365138/94‑1 (MQ=60)

                                                               GCAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCAC                                >  SRR4302547.5644833/1‑94 (MQ=60)

                                                                CAAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACC                               >  SRR4302547.782663/1‑94 (MQ=60)

                                                                CAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACC                               <  SRR4302547.533912/94‑1 (MQ=60)

                                                                 AAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCC                              <  SRR4302547.2595714/94‑1 (MQ=60)

                                                                 AAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCC                              >  SRR4302547.3119115/1‑94 (MQ=60)

                                                                 AAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCC                              <  SRR4302547.795618/94‑1 (MQ=60)

                                                                 AAACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGGTCTTTCAATGGCCAATGATACCACTGGATTCACag                              >  SRR4302547.1832393/1‑92 (MQ=60)

                                                                 AAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCC                              >  SRR4302547.3901608/1‑94 (MQ=60)

                                                                  AACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCG                             >  SRR4302547.1265091/1‑94 (MQ=60)

                                                                  AACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCG                             >  SRR4302547.4757080/1‑94 (MQ=60)

                                                                  AACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCG                             <  SRR4302547.3842534/94‑1 (MQ=60)

                                                                  AACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCG                             >  SRR4302547.4940462/1‑94 (MQ=60)

                                                                   ACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGA                            >  SRR4302547.4828904/1‑94 (MQ=60)

                                                                   ACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGA                            >  SRR4302547.2278298/1‑94 (MQ=60)

                                                                   ACCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGA                            <  SRR4302547.2414673/94‑1 (MQ=60)

                                                                    CCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAA                           >  SRR4302547.1835320/1‑94 (MQ=60)

                                                                    CCCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAA                           >  SRR4302547.5894669/1‑94 (MQ=60)

                                                                     CCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAAT                          <  SRR4302547.4760436/94‑1 (MQ=60)

                                                                     CCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAAT                          >  SRR4302547.5739340/1‑94 (MQ=60)

                                                                     CCGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAAT                          <  SRR4302547.3134616/94‑1 (MQ=60)

                                                                     CCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAAT                          <  SRR4302547.6320189/94‑1 (MQ=60)

                                                                      CGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATT                         <  SRR4302547.490739/94‑1 (MQ=60)

                                                                      CGAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATT                         >  SRR4302547.2330180/1‑94 (MQ=60)

                                                                       GAATCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTA                        <  SRR4302547.2463705/94‑1 (MQ=60)

                                                                          TCATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTCCAATGGCCAATAATACCACTGGATTCACCCGAATTATCA                     <  SRR4302547.5755961/94‑1 (MQ=60)

                                                                           CATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAA                    >  SRR4302547.4222963/1‑94 (MQ=60)

                                                                            ATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAA                   >  SRR4302547.4122273/1‑94 (MQ=60)

                                                                            ATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAA                   >  SRR4302547.5324711/1‑94 (MQ=60)

                                                                            ATCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCAACCGAATTATCAAA                   >  SRR4302547.465533/1‑94 (MQ=60)

                                                                            ATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAA                   >  SRR4302547.5295157/1‑94 (MQ=60)

                                                                             TCGGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAG                  >  SRR4302547.3998302/1‑94 (MQ=60)

                                                                               GGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCT                >  SRR4302547.965021/1‑94 (MQ=60)

                                                                               GGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCT                <  SRR4302547.4316050/94‑1 (MQ=60)

                                                                               GGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCT                >  SRR4302547.646264/1‑94 (MQ=60)

                                                                               GGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCT                <  SRR4302547.2992897/94‑1 (MQ=60)

                                                                               GGATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCT                >  SRR4302547.6066060/1‑94 (MQ=60)

                                                                               GGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCT                >  SRR4302547.858977/1‑94 (MQ=60)

                                                                               GGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCT                <  SRR4302547.6708074/94‑1 (MQ=60)

                                                                                GATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTG               >  SRR4302547.4594054/1‑94 (MQ=60)

                                                                                GATTTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTG               <  SRR4302547.2631353/94‑1 (MQ=60)

                                                                                   TTAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTG            <  SRR4302547.2885251/94‑1 (MQ=60)

                                                                                    TAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGG           >  SRR4302547.2441812/1‑94 (MQ=60)

                                                                                    TAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGG           <  SRR4302547.1943467/94‑1 (MQ=60)

                                                                                    TAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGG           >  SRR4302547.2753682/1‑94 (MQ=60)

                                                                                    TAACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGG           >  SRR4302547.60261/1‑94 (MQ=60)

                                                                                     AACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGC          <  SRR4302547.6447933/94‑1 (MQ=60)

                                                                                     AACGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGC          >  SRR4302547.389719/1‑94 (MQ=60)

                                                                                       CGGTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTA        >  SRR4302547.1486548/1‑94 (MQ=60)

                                                                                         GTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATT      <  SRR4302547.4473320/94‑1 (MQ=60)

                                                                                         GTACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATT      >  SRR4302547.5556945/1‑94 (MQ=60)

                                                                                          TACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTC     >  SRR4302547.3842176/1‑94 (MQ=60)

                                                                                           ACGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCC    >  SRR4302547.5968990/1‑94 (MQ=60)

                                                                                            CGCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCT   >  SRR4302547.4228455/1‑94 (MQ=60)

                                                                                            CACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCT   >  SRR4302547.3909700/1‑94 (MQ=60)

                                                                                             GCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTG  >  SRR4302547.3278663/1‑94 (MQ=60)

                                                                                             GCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTG  >  SRR4302547.3203209/1‑94 (MQ=60)

                                                                                             GCTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTG  >  SRR4302547.2698457/1‑94 (MQ=60)

GCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTG  >  NC_000913/4254085‑4254271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`