Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,416,484 A→G H242H (CAT→CAC)  yjfR ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,416,4840AG84.8% 661.1 / 99.2 258H242H (CAT→CAC) yjfRhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/21);  new base G (115/103);  total (133/125)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCA  >  NC_000913/4416392‑4416575
                                                                                            |                                                                                           
    cccccATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGtt                                                                                         <  1:6465975/91‑1 (MQ=255)
    cACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGtt                                                                                         <  2:2432880/93‑1 (MQ=255)
    cACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGtt                                                                                         <  2:1669555/93‑1 (MQ=255)
     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTc                                                                                        >  1:1081488/1‑93 (MQ=255)
     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTc                                                                                        >  2:2897495/1‑93 (MQ=255)
     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTc                                                                                        >  1:4650735/1‑93 (MQ=255)
     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTc                                                                                        >  2:3521530/1‑93 (MQ=255)
     aCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTc                                                                                        >  2:4617453/1‑93 (MQ=255)
        cATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGtt                                                                                     >  2:5233469/1‑93 (MQ=255)
          tACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTAc                                                                                   <  1:2456787/93‑1 (MQ=255)
            cGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCg                                                                                 >  1:858062/1‑93 (MQ=255)
             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                <  1:1797524/93‑1 (MQ=255)
             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                >  2:389303/1‑93 (MQ=255)
             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                <  2:5921606/93‑1 (MQ=255)
             gcagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGt                                                                                >  2:3042672/1‑93 (MQ=255)
              cagcaTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGtc                                                                               >  1:2209956/1‑92 (MQ=255)
                 caTATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCtt                                                                            <  1:4579095/93‑1 (MQ=255)
                   tatCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACGGTGCTTCg                                                                          >  2:4860071/1‑93 (MQ=255)
                   tatCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCg                                                                          >  2:4514794/1‑93 (MQ=255)
                    atCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGc                                                                         >  1:1190227/1‑93 (MQ=255)
                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       <  2:4787801/93‑1 (MQ=255)
                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       >  1:1541743/1‑93 (MQ=255)
                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       <  1:1457055/93‑1 (MQ=255)
                      cGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCat                                                                       >  2:1015978/1‑93 (MQ=255)
                       ggCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCata                                                                      >  1:3907124/1‑93 (MQ=255)
                        gcgcTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCataa                                                                     >  1:5251577/1‑93 (MQ=255)
                          gcTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCataata                                                                   >  1:3757102/1‑93 (MQ=255)
                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  >  1:700304/1‑93 (MQ=255)
                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  <  2:4129251/93‑1 (MQ=255)
                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  >  1:535017/1‑93 (MQ=255)
                           cTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAg                                                                  <  2:6469731/93‑1 (MQ=255)
                            tGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGt                                                                 <  2:4393743/93‑1 (MQ=255)
                             ggTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGtt                                                                <  2:923209/93‑1 (MQ=255)
                               tCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTag                                                              >  2:5319627/1‑93 (MQ=255)
                                cATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTaga                                                             <  2:6074751/93‑1 (MQ=255)
                                   tttGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGagta                                                          <  2:318634/93‑1 (MQ=255)
                                     tGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGagtagt                                                        >  2:110167/1‑93 (MQ=255)
                                     tGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGagtagt                                                        <  1:4205434/93‑1 (MQ=255)
                                      gTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTg                                                       <  2:1391608/93‑1 (MQ=255)
                                      gTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTg                                                       <  2:5708159/93‑1 (MQ=255)
                                       tCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTgg                                                      >  2:6028048/1‑93 (MQ=255)
                                        cGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTggg                                                     <  2:1240005/93‑1 (MQ=255)
                                          gTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAg                                                   >  2:4193905/1‑93 (MQ=255)
                                          gTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAg                                                   >  1:4740853/1‑93 (MQ=255)
                                          gTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAg                                                   <  1:2421539/93‑1 (MQ=255)
                                           tGATACCGCGCGTGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGt                                                  <  1:3685147/93‑1 (MQ=255)
                                           tGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGt                                                  >  1:736707/1‑93 (MQ=255)
                                           tGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGt                                                  >  2:2469870/1‑93 (MQ=255)
                                            gATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTc                                                 <  1:1720621/93‑1 (MQ=255)
                                             aTACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcg                                                >  2:5425877/1‑93 (MQ=255)
                                              tACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgc                                               <  1:5571654/93‑1 (MQ=255)
                                              tACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgc                                               <  1:3954786/93‑1 (MQ=255)
                                               actgcgcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgcc                                              >  1:4274149/4‑93 (MQ=255)
                                               aCCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgcc                                              <  1:4994107/93‑1 (MQ=255)
                                                ccGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgccg                                             >  1:6416726/1‑93 (MQ=255)
                                                 cgcgcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgccgc                                            >  2:2017938/1‑93 (MQ=255)
                                                   cgcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtg                                          >  1:3452750/1‑93 (MQ=255)
                                                    gcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgt                                         >  1:2155813/1‑93 (MQ=255)
                                                    gcgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgt                                         <  2:3136746/93‑1 (MQ=255)
                                                     cgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgtg                                        >  1:1627083/1‑93 (MQ=255)
                                                     cgGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCtgtg                                        >  2:6631920/1‑93 (MQ=255)
                                                       ggTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGAt                                      >  2:3328554/1‑93 (MQ=255)
                                                        gTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATa                                     >  1:5809285/1‑93 (MQ=255)
                                                         ttttCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATAc                                    <  2:684511/93‑1 (MQ=255)
                                                           ttCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAg                                  <  1:5021974/93‑1 (MQ=255)
                                                            tCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAgg                                 <  2:3466784/93‑1 (MQ=255)
                                                            tCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAgg                                 <  2:4232513/93‑1 (MQ=255)
                                                             cGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAggg                                >  1:1671352/1‑93 (MQ=255)
                                                             cGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAggg                                <  1:2867299/93‑1 (MQ=255)
                                                             cGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAggg                                <  1:2611727/93‑1 (MQ=255)
                                                              gCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGa                               <  1:5169146/93‑1 (MQ=255)
                                                                cGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  1:604784/1‑93 (MQ=255)
                                                                cGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  2:6308212/1‑93 (MQ=255)
                                                                cGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  2:5081152/1‑93 (MQ=255)
                                                                cGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgc                             >  2:2652522/1‑93 (MQ=255)
                                                                 gTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgcc                            <  1:2340571/93‑1 (MQ=255)
                                                                   tcGATCCTAACGCCACGTCGATCTGTTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgccgc                          <  1:4322964/92‑1 (MQ=255)
                                                                    cGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgccgcc                         <  1:5960256/93‑1 (MQ=255)
                                                                      aTCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAg                       <  2:3698533/93‑1 (MQ=255)
                                                                          tAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGt                   <  2:898067/93‑1 (MQ=255)
                                                                           aaCGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtt                  <  2:258275/93‑1 (MQ=255)
                                                                            aCGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGttt                 <  2:5035692/93‑1 (MQ=255)
                                                                            aCGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGttt                 <  1:4080196/93‑1 (MQ=255)
                                                                             cGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                <  2:4727205/93‑1 (MQ=255)
                                                                             cGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                >  2:2219260/1‑93 (MQ=255)
                                                                             cGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                >  2:322594/1‑93 (MQ=255)
                                                                             cGCCACGTCGATCTGGTGTTCGTCACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGtttt                <  2:3615515/93‑1 (MQ=255)
                                                                              gCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCAGCCAGGCGTTTTg               >  1:3333583/1‑93 (MQ=255)
                                                                               ccACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGa              >  1:5827844/1‑93 (MQ=255)
                                                                                cACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGaa             <  1:3076823/93‑1 (MQ=255)
                                                                                   gTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAg          >  1:3609884/1‑93 (MQ=255)
                                                                                   gTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAg          >  1:1002658/1‑93 (MQ=255)
                                                                                     cGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGt        <  2:1906567/93‑1 (MQ=255)
                                                                                      gATCTGGTTTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTa       <  2:612998/93‑1 (MQ=255)
                                                                                      gATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTa       <  2:1548685/93‑1 (MQ=255)
                                                                                       aTCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAg      >  1:2551674/1‑93 (MQ=255)
                                                                                       aTCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTCAACAGGTAg      >  2:2511208/1‑93 (MQ=255)
                                                                                       aTCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAg      <  2:3990289/93‑1 (MQ=255)
                                                                                        tCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGt     <  1:451143/93‑1 (MQ=255)
                                                                                         cTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGtt    >  2:2775677/1‑93 (MQ=255)
                                                                                            |                                                                                           
GCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCA  >  NC_000913/4416392‑4416575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACC  >  NC_000913/4416391‑4416577
                                                                                             |                                                                                             
CGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGG                                                                                               >  SRR4302547.1869573/1‑94 (MQ=60)
CGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGG                                                                                               >  SRR4302547.6528341/1‑94 (MQ=60)
 GCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGT                                                                                              <  SRR4302547.1546377/94‑1 (MQ=60)
  CTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTG                                                                                             <  SRR4302547.5257682/94‑1 (MQ=60)
   TTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGT                                                                                            <  SRR4302547.3987873/94‑1 (MQ=60)
   TTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGT                                                                                            >  SRR4302547.6564422/1‑94 (MQ=60)
    TCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTT                                                                                           >  SRR4302547.5223959/1‑94 (MQ=60)
     CCCCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTC                                                                                          <  SRR4302547.6600616/94‑1 (MQ=60)
     CACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTC                                                                                          >  SRR4302547.1103083/1‑94 (MQ=60)
     CACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTC                                                                                          >  SRR4302547.4747067/1‑94 (MQ=60)
     CACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTC                                                                                          <  SRR4302547.82442/94‑1 (MQ=60)
     CACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTC                                                                                          >  SRR4302547.4797652/1‑94 (MQ=60)
     CACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTC                                                                                          >  SRR4302547.5173635/1‑94 (MQ=60)
      ACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCG                                                                                         >  SRR4302547.6120341/1‑94 (MQ=60)
       CCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGT                                                                                        >  SRR4302547.1806298/1‑94 (MQ=60)
       CCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACATCGATCTGGGGTTCGT                                                                                        >  SRR4302547.966954/1‑94 (MQ=60)
        CCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTT                                                                                       >  SRR4302547.4746690/1‑94 (MQ=60)
           TACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACC                                                                                    >  SRR4302547.4955868/1‑94 (MQ=60)
           TACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACC                                                                                    <  SRR4302547.2506436/94‑1 (MQ=60)
           TACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACC                                                                                    <  SRR4302547.1152358/94‑1 (MQ=60)
            ACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCG                                                                                   >  SRR4302547.874460/1‑94 (MQ=60)
             CGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGT                                                                                  <  SRR4302547.6162906/94‑1 (MQ=60)
              GCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTG                                                                                 <  SRR4302547.1833084/94‑1 (MQ=60)
              GCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTC                                                                                 >  SRR4302547.2254389/1‑94 (MQ=60)
               CAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGCTAACGTGC                                                                                >  SRR4302547.4049192/1‑94 (MQ=60)
               CAGCATATCGGCGCTGGGCATTTTGTCGGTGATACCGCGCGGGGTTTTGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACAGTGC                                                                                >  SRR4302547.4728420/1‑94 (MQ=60)
                AGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCT                                                                               >  SRR4302547.1853815/1‑94 (MQ=60)
                AGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCT                                                                               >  SRR4302547.5314339/1‑94 (MQ=60)
                 GCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTT                                                                              <  SRR4302547.6656165/94‑1 (MQ=60)
                  CATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTC                                                                             <  SRR4302547.4673866/94‑1 (MQ=60)
                  CATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTC                                                                             >  SRR4302547.1140124/1‑94 (MQ=60)
                   ATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCG                                                                            >  SRR4302547.4554315/1‑94 (MQ=60)
                   ATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCG                                                                            >  SRR4302547.2495334/1‑94 (MQ=60)
                   ATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCG                                                                            >  SRR4302547.3017258/1‑94 (MQ=60)
                    TATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGC                                                                           >  SRR4302547.1214249/1‑94 (MQ=60)
                      TCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCAT                                                                         >  SRR4302547.1572431/1‑94 (MQ=60)
                       CGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATA                                                                        <  SRR4302547.1622205/94‑1 (MQ=60)
                       CGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATA                                                                        >  SRR4302547.3986584/1‑94 (MQ=60)
                       CGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATA                                                                        <  SRR4302547.1486174/94‑1 (MQ=60)
                        GGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAA                                                                       >  SRR4302547.5359709/1‑94 (MQ=60)
                         GCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAAT                                                                      <  SRR4302547.5280374/94‑1 (MQ=60)
                          CGCTGGTGATTTTGTCGGTGATACCGCGCGGGTGTTCGCCGTACGATCCTTACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATA                                                                     >  SRR4302547.415729/1‑94 (MQ=60)
                          CGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATA                                                                     >  SRR4302547.3833753/1‑94 (MQ=60)
                           GCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAG                                                                    >  SRR4302547.544685/1‑94 (MQ=60)
                           GCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAG                                                                    >  SRR4302547.1826600/1‑94 (MQ=60)
                           GCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAG                                                                    >  SRR4302547.713245/1‑94 (MQ=60)
                            CTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGT                                                                   >  SRR4302547.2625632/1‑94 (MQ=60)
                            CTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGT                                                                   <  SRR4302547.1951733/94‑1 (MQ=60)
                             TGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTT                                                                  >  SRR4302547.296692/1‑94 (MQ=60)
                             TGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTT                                                                  >  SRR4302547.4659223/1‑94 (MQ=60)
                               GTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAG                                                                <  SRR4302547.656296/94‑1 (MQ=60)
                               GTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAG                                                                >  SRR4302547.379937/1‑94 (MQ=60)
                                TCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGA                                                               <  SRR4302547.1056621/94‑1 (MQ=60)
                                TCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGA                                                               >  SRR4302547.2231000/1‑94 (MQ=60)
                                  ATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGT                                                             >  SRR4302547.6715368/1‑94 (MQ=60)
                                   TTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTA                                                            <  SRR4302547.3531053/94‑1 (MQ=60)
                                    TTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAG                                                           >  SRR4302547.3531279/1‑94 (MQ=60)
                                     TTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGT                                                          >  SRR4302547.5579813/1‑94 (MQ=60)
                                      TGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTG                                                         >  SRR4302547.4300089/1‑94 (MQ=60)
                                      TGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTG                                                         <  SRR4302547.4291240/94‑1 (MQ=60)
                                         CGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGA                                                      >  SRR4302547.4378004/1‑94 (MQ=60)
                                          GGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAG                                                     >  SRR4302547.4839108/1‑94 (MQ=60)
                                          GGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAG                                                     <  SRR4302547.4322513/94‑1 (MQ=60)
                                           GTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGT                                                    >  SRR4302547.750494/1‑94 (MQ=60)
                                           GTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGT                                                    <  SRR4302547.2470556/94‑1 (MQ=60)
                                            TGATACCGCGCGTGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTC                                                   <  SRR4302547.3760378/94‑1 (MQ=60)
                                            TGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTC                                                   <  SRR4302547.3188147/94‑1 (MQ=60)
                                            TGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTC                                                   <  SRR4302547.1571178/94‑1 (MQ=60)
                                            TGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTC                                                   >  SRR4302547.245221/1‑94 (MQ=60)
                                            TGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTC                                                   <  SRR4302547.4631578/94‑1 (MQ=60)
                                             GATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCG                                                  <  SRR4302547.1754443/94‑1 (MQ=60)
                                               TACTGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCC                                                >  SRR4302547.4361912/1‑94 (MQ=60)
                                               TACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCC                                                <  SRR4302547.5686544/94‑1 (MQ=60)
                                               TACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCC                                                <  SRR4302547.4035230/94‑1 (MQ=60)
                                               TACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCC                                                <  SRR4302547.4497709/94‑1 (MQ=60)
                                                ACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCG                                               <  SRR4302547.5097362/94‑1 (MQ=60)
                                                ACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCG                                               >  SRR4302547.6550522/1‑94 (MQ=60)
                                                  CGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCT                                             <  SRR4302547.508892/94‑1 (MQ=60)
                                                   GCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTG                                            >  SRR4302547.373238/1‑94 (MQ=60)
                                                   GCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTG                                            >  SRR4302547.3523143/1‑94 (MQ=60)
                                                    CGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGT                                           >  SRR4302547.2198874/1‑94 (MQ=60)
                                                     GCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTG                                          >  SRR4302547.1659272/1‑94 (MQ=60)
                                                     GCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTG                                          >  SRR4302547.3901977/1‑94 (MQ=60)
                                                     GCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTG                                          <  SRR4302547.1355291/94‑1 (MQ=60)
                                                      CGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGA                                         <  SRR4302547.3837303/94‑1 (MQ=60)
                                                      CGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGA                                         <  SRR4302547.4948078/94‑1 (MQ=60)
                                                        GGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATA                                       >  SRR4302547.5929472/1‑94 (MQ=60)
                                                           TTTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAG                                    <  SRR4302547.2738695/94‑1 (MQ=60)
                                                            TTCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGG                                   <  SRR4302547.5125743/94‑1 (MQ=60)
                                                             TCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGG                                  <  SRR4302547.5385425/94‑1 (MQ=60)
                                                             TCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGG                                  <  SRR4302547.925613/94‑1 (MQ=60)
                                                             TCGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGG                                  >  SRR4302547.1704278/1‑94 (MQ=60)
                                                              CGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGA                                 >  SRR4302547.3710126/1‑94 (MQ=60)
                                                              CGCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGA                                 <  SRR4302547.2924576/94‑1 (MQ=60)
                                                              CGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGA                                 <  SRR4302547.2664354/94‑1 (MQ=60)
                                                               GCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAG                                <  SRR4302547.5799109/94‑1 (MQ=60)
                                                               GCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAG                                <  SRR4302547.5275581/94‑1 (MQ=60)
                                                               GCCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAG                                <  SRR4302547.2360189/94‑1 (MQ=60)
                                                                CCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGC                               >  SRR4302547.5662703/1‑94 (MQ=60)
                                                                CCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGC                               >  SRR4302547.5932355/1‑94 (MQ=60)
                                                                CCGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGC                               >  SRR4302547.616018/1‑94 (MQ=60)
                                                                 CGTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCC                              >  SRR4302547.794956/1‑94 (MQ=60)
                                                                 CGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCC                              <  SRR4302547.6038080/94‑1 (MQ=60)
                                                                  GTACGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCG                             <  SRR4302547.2388016/94‑1 (MQ=60)
                                                                   TACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGC                            >  SRR4302547.4174274/1‑94 (MQ=60)
                                                                    TCGATCCTAACGCCACGTCGATCTGTTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCC                           <  SRR4302547.4411959/94‑1 (MQ=60)
                                                                     CGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCA                          <  SRR4302547.6083848/94‑1 (MQ=60)
                                                                     CGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCA                          <  SRR4302547.3502931/94‑1 (MQ=60)
                                                                     CGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCA                          <  SRR4302547.597428/94‑1 (MQ=60)
                                                                     CGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCA                          <  SRR4302547.4020358/94‑1 (MQ=60)
                                                                     CGATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCA                          <  SRR4302547.2562817/94‑1 (MQ=60)
                                                                     CGATCCTAACGCCACGTCAATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCA                          <  SRR4302547.1689834/94‑1 (MQ=60)
                                                                      GATCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAG                         >  SRR4302547.1576393/1‑94 (MQ=60)
                                                                        TCCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGC                       <  SRR4302547.147145/94‑1 (MQ=60)
                                                                         CCTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCG                      >  SRR4302547.2396944/1‑94 (MQ=60)
                                                                          CTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGT                     >  SRR4302547.232090/1‑94 (MQ=60)
                                                                          CTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGT                     >  SRR4302547.2652246/1‑94 (MQ=60)
                                                                          CTAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGT                     >  SRR4302547.4203574/1‑94 (MQ=60)
                                                                           TAACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTT                    >  SRR4302547.1711501/1‑94 (MQ=60)
                                                                            AACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTT                   >  SRR4302547.5737426/1‑94 (MQ=60)
                                                                             ACGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTT                  <  SRR4302547.4163114/94‑1 (MQ=60)
                                                                              CGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTG                 >  SRR4302547.3316319/1‑94 (MQ=60)
                                                                              CGCCACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCAGCCAGGCGTTTTG                 >  SRR4302547.3401001/1‑94 (MQ=60)
                                                                               GCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGA                >  SRR4302547.5948451/1‑94 (MQ=60)
                                                                                 CACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAAC              <  SRR4302547.63309/94‑1 (MQ=60)
                                                                                 CACGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAAC              <  SRR4302547.3137937/94‑1 (MQ=60)
                                                                                  ACGACGATCTGGTGTTCGTTACCGTGCTTCGCGTAATAGTTAGAGTAGTGGGAGTTGCCGCTGTGATTCAGGGAGCCGCCAGGCGTTTTGAACA             <  SRR4302547.3340163/94‑1 (MQ=60)
                                                                                   CGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAG            >  SRR4302547.1022222/1‑94 (MQ=60)
                                                                                   CGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAG            >  SRR4302547.3683504/1‑94 (MQ=60)
                                                                                   CGTCGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAG            >  SRR4302547.4778407/1‑94 (MQ=60)
                                                                                      CGATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGAA         >  SRR4302547.2588300/1‑94 (MQ=60)
                                                                                       GATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAG        >  SRR4302547.733585/1‑94 (MQ=60)
                                                                                       GATCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAG        >  SRR4302547.2603201/1‑94 (MQ=60)
                                                                                         TCTGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTT      <  SRR4302547.1669120/94‑1 (MQ=60)
                                                                                         TCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTT      <  SRR4302547.459513/94‑1 (MQ=60)
                                                                                           TGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCA    >  SRR4302547.745234/1‑94 (MQ=60)
                                                                                           TGGTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCA    <  SRR4302547.2787581/94‑1 (MQ=60)
                                                                                             GTGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCATC  >  SRR4302547.2318663/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACC  >  NC_000913/4416391‑4416577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑