Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,429,526	C→T	A12A (GCG→GCA)	ytfE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,429,526	0	C	T	99.7%	1124.6 / NA	295	A12A (GCG→GCA)	ytfE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (130/164); minor base G (1/0); total (131/164)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

TGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGG  >  NC_000913/4429436‑4429615

tGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAgtgc                                                                                         <  1:5464200/93‑1 (MQ=255)

  ccgccgCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCa                                                                                       >  2:5644421/1‑93 (MQ=255)

    gccgcGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGt                                                                                     <  1:1030612/93‑1 (MQ=255)

    cccgcGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGt                                                                                     <  2:1196290/92‑1 (MQ=255)

       gcgcgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCa                                                                                  <  2:6171475/93‑1 (MQ=255)

       gcgcgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCa                                                                                  >  2:1514438/1‑93 (MQ=255)

       gcgcgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCa                                                                                  >  1:4438941/1‑93 (MQ=255)

        cgcgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCAc                                                                                 <  1:6553140/93‑1 (MQ=255)

         gcgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCAcc                                                                                <  2:5833693/93‑1 (MQ=255)

         gcgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCAcc                                                                                >  2:3422109/1‑93 (MQ=255)

         gcgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCAcc                                                                                <  2:2502450/93‑1 (MQ=255)

          cgcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCt                                                                               >  1:5716104/1‑93 (MQ=255)

           gcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTa                                                                              >  2:662763/1‑93 (MQ=255)

           gcgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTa                                                                              <  2:465097/93‑1 (MQ=255)

            cgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTaa                                                                             >  1:1174230/1‑93 (MQ=255)

             gcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTaaa                                                                            <  1:2373657/93‑1 (MQ=255)

              ccAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAg                                                                           >  2:1883646/1‑93 (MQ=255)

               cAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAgg                                                                          >  1:4260839/1‑93 (MQ=255)

                aGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGt                                                                         >  1:1788392/1‑93 (MQ=255)

                 gCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGtt                                                                        <  1:3245515/93‑1 (MQ=255)

                 gCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGtt                                                                        >  2:2060131/1‑93 (MQ=255)

                 gCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGtt                                                                        <  2:508298/93‑1 (MQ=255)

                 gCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGtt                                                                        >  1:3136563/1‑93 (MQ=255)

                 gCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGtt                                                                        <  1:804644/93‑1 (MQ=255)

                  cGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAGGGTTg                                                                       <  1:4205987/93‑1 (MQ=255)

                   gTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTgg                                                                      >  1:3571670/1‑93 (MQ=255)

                      tGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTcg                                                                   <  1:3255496/93‑1 (MQ=255)

                      tGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTcg                                                                   >  1:5400422/1‑93 (MQ=255)

                       gCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTcgc                                                                  >  2:142899/1‑93 (MQ=255)

                       gCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTcgc                                                                  >  2:2223163/1‑93 (MQ=255)

                          tACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGAt                                                               <  1:2749883/93‑1 (MQ=255)

                            ccGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATaa                                                             <  2:614637/93‑1 (MQ=255)

                                 acaGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCAt                                                        <  2:6114969/93‑1 (MQ=255)

                                  cagcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATa                                                       <  1:4062283/93‑1 (MQ=255)

                                  cagcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATa                                                       <  2:2623841/93‑1 (MQ=255)

                                  cagcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATa                                                       >  1:5739790/1‑93 (MQ=255)

                                   agcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATCAGCCATAg                                                      >  2:1802094/1‑93 (MQ=255)

                                   agcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAg                                                      <  2:5355054/93‑1 (MQ=255)

                                   agcagTAATCCATATCATACTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAg                                                      >  1:6500138/1‑93 (MQ=255)

                                    gcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGc                                                     <  2:3324609/93‑1 (MQ=255)

                                    gcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGc                                                     <  1:5118794/93‑1 (MQ=255)

                                    gcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGc                                                     >  2:1622789/1‑93 (MQ=255)

                                    gcagTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGc                                                     <  2:3542280/93‑1 (MQ=255)

                                     cagTAATCCATATCATATCTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCt                                                    <  2:3648303/93‑1 (MQ=255)

                                        tAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGAt                                                 <  1:1434317/93‑1 (MQ=255)

                                         aaTCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATa                                                <  2:1931771/93‑1 (MQ=255)

                                          aTCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAc                                               >  1:2291064/1‑93 (MQ=255)

                                           tCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAcc                                              >  2:4912219/1‑93 (MQ=255)

                                            ccATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCt                                             <  1:1270579/93‑1 (MQ=255)

                                            ccATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCt                                             <  1:3429853/93‑1 (MQ=255)

                                             catatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTc                                            >  1:2222803/1‑93 (MQ=255)

                                              atatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCa                                           >  2:5918417/1‑93 (MQ=255)

                                              atatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCa                                           <  2:386387/93‑1 (MQ=255)

                                               tatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAt                                          >  1:3801340/1‑93 (MQ=255)

                                                 tcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTc                                        >  1:3893787/1‑93 (MQ=255)

                                                 tcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTc                                        >  2:3022431/1‑93 (MQ=255)

                                                  catatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCt                                       >  1:6205339/1‑93 (MQ=255)

                                                  catatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCt                                       >  1:3361019/1‑93 (MQ=255)

                                                   atatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCtt                                      >  1:6348673/1‑93 (MQ=255)

                                                   atatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCtt                                      >  1:588235/1‑93 (MQ=255)

                                                    tatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTta                                     <  2:808176/93‑1 (MQ=255)

                                                     atTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaa                                    <  1:6087880/93‑1 (MQ=255)

                                                       ttACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaata                                  <  2:6025120/93‑1 (MQ=255)

                                                        tACGAAACAGAGCTGAAGCGCGAGCAATAGAGAGTGCCAGTTCCCCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaataa                                 <  2:6551636/93‑1 (MQ=255)

                                                         aCGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataag                                <  2:3362332/93‑1 (MQ=255)

                                                         aCGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataag                                <  2:5589989/93‑1 (MQ=255)

                                                          cGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataaga                               <  2:5690026/93‑1 (MQ=255)

                                                          cGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataaga                               <  1:3205222/93‑1 (MQ=255)

                                                           gAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagat                              <  2:1307497/93‑1 (MQ=255)

                                                            aaaCAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagata                             <  1:2914306/93‑1 (MQ=255)

                                                             aaCAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagataa                            <  1:2135314/93‑1 (MQ=255)

                                                             aaCAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagataa                            <  2:5456184/93‑1 (MQ=255)

                                                              aCAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagataag                           >  2:4110544/1‑93 (MQ=255)

                                                              aCAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagataag                           <  1:3739679/93‑1 (MQ=255)

                                                               cAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAAtaagataaga                          <  2:308560/93‑1 (MQ=255)

                                                                agagCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATaagataagat                         <  1:602826/93‑1 (MQ=255)

                                                                 gagCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATg                        >  1:4646175/1‑93 (MQ=255)

                                                                 gagCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATg                        >  1:6385252/1‑93 (MQ=255)

                                                                 gagCTGAAGCGCGAGAAATAGAGAGTGCCACTTCACCTAAAGGTGGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATg                        <  1:201298/93‑1 (MQ=255)

                                                                  agCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGt                       <  1:5240283/93‑1 (MQ=255)

                                                                  agCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGt                       >  2:511272/1‑93 (MQ=255)

                                                                   gCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTa                      <  2:2276321/93‑1 (MQ=255)

                                                                    cTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTAt                     <  1:5327259/93‑1 (MQ=255)

                                                                       aaGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTAtttt                  <  1:4704865/93‑1 (MQ=255)

                                                                        aGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTa                 >  2:4807367/1‑93 (MQ=255)

                                                                        aGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTa                 <  2:3203742/93‑1 (MQ=255)

                                                                          cgcgAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTaaa               <  2:4683539/93‑1 (MQ=255)

                                                                          cgcgAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTaaa               <  1:5585812/93‑1 (MQ=255)

                                                                             gAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGc            <  1:1980161/93‑1 (MQ=255)

                                                                              aGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCa           >  2:5476052/1‑93 (MQ=255)

                                                                               ggAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCAt          >  2:4376535/1‑93 (MQ=255)

                                                                                gAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATc         <  2:3974130/93‑1 (MQ=255)

                                                                                 aaTAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCt        <  2:4269195/93‑1 (MQ=255)

                                                                                 aaTAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCt        <  2:6239062/93‑1 (MQ=255)

                                                                                  aTAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCtt       <  1:2490645/93‑1 (MQ=255)

                                                                                   tAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCttt      <  1:885656/93‑1 (MQ=255)

                                                                                    agagagTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTa     >  1:729141/1‑93 (MQ=255)

                                                                                     gagagTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTaa    >  1:4864181/1‑93 (MQ=255)

                                                                                      agagTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAg   <  2:3915506/93‑1 (MQ=255)

                                                                                       gagTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAgg  <  2:552857/93‑1 (MQ=255)

TGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGG  >  NC_000913/4429436‑4429615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAA  >  NC_000913/4429433‑4429618

ACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGT                                                                                              <  SRR4302547.3144793/94‑1 (MQ=60)

 CGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTG                                                                                             <  SRR4302547.5648745/94‑1 (MQ=60)

 CGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTG                                                                                             <  SRR4302547.2156188/94‑1 (MQ=60)

  GTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGC                                                                                            >  SRR4302547.3452380/1‑94 (MQ=60)

  GTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGC                                                                                            >  SRR4302547.6371755/1‑94 (MQ=60)

  GTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGC                                                                                            >  SRR4302547.759116/1‑94 (MQ=60)

   TGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCC                                                                                           <  SRR4302547.5577079/94‑1 (MQ=60)

   TGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCC                                                                                           <  SRR4302547.1918404/94‑1 (MQ=60)

    GCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCA                                                                                          <  SRR4302547.580349/94‑1 (MQ=60)

     CCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAG                                                                                         <  SRR4302547.3309446/94‑1 (MQ=60)

      CGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGT                                                                                        <  SRR4302547.2644175/94‑1 (MQ=60)

      CGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGT                                                                                        >  SRR4302547.2417940/1‑94 (MQ=60)

       GCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTT                                                                                       <  SRR4302547.1050864/94‑1 (MQ=60)

        CCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTC                                                                                      <  SRR4302547.2359080/94‑1 (MQ=60)

         CGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCA                                                                                     >  SRR4302547.3961205/1‑94 (MQ=60)

         CGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCA                                                                                     >  SRR4302547.4530725/1‑94 (MQ=60)

         CGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCA                                                                                     >  SRR4302547.5436539/1‑94 (MQ=60)

          GCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCAC                                                                                    <  SRR4302547.3822998/94‑1 (MQ=60)

          GCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCAC                                                                                    >  SRR4302547.5998028/1‑94 (MQ=60)

           CGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACC                                                                                   <  SRR4302547.6689550/94‑1 (MQ=60)

            GCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCT                                                                                  <  SRR4302547.1176111/94‑1 (MQ=60)

            GCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCT                                                                                  >  SRR4302547.5834155/1‑94 (MQ=60)

            GCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCT                                                                                  <  SRR4302547.831668/94‑1 (MQ=60)

             CGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTA                                                                                 <  SRR4302547.5329923/94‑1 (MQ=60)

              GCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAA                                                                                >  SRR4302547.1197960/1‑94 (MQ=60)

                GCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAG                                                                              <  SRR4302547.2421760/94‑1 (MQ=60)

                GCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAG                                                                              <  SRR4302547.6508392/94‑1 (MQ=60)

                GCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAG                                                                              <  SRR4302547.6158731/94‑1 (MQ=60)

                GCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGATGAAGCGCGAGGAATAGAGAGGGCGAGTTCACCTAAAG                                                                              >  SRR4302547.4761606/1‑94 (MQ=60)

                 CCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGG                                                                             >  SRR4302547.583138/1‑94 (MQ=60)

                 CCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGG                                                                             >  SRR4302547.4348029/1‑94 (MQ=60)

                 CCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGG                                                                             <  SRR4302547.1215073/94‑1 (MQ=60)

                 CCAGCGACTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGG                                                                             >  SRR4302547.4292025/1‑94 (MQ=60)

                  CAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGT                                                                            >  SRR4302547.2019519/1‑94 (MQ=60)

                  CAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGT                                                                            >  SRR4302547.1823794/1‑94 (MQ=60)

                   AGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTT                                                                           >  SRR4302547.3198858/1‑94 (MQ=60)

                   AGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTT                                                                           >  SRR4302547.4128856/1‑94 (MQ=60)

                    GCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTG                                                                          <  SRR4302547.819892/94‑1 (MQ=60)

                    GCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTG                                                                          <  SRR4302547.1983353/94‑1 (MQ=60)

                    GCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTG                                                                          <  SRR4302547.3310349/94‑1 (MQ=60)

                     CGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAGGGTTGG                                                                         <  SRR4302547.4291812/94‑1 (MQ=60)

                     CGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGG                                                                         <  SRR4302547.5740513/94‑1 (MQ=60)

                     CGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGG                                                                         <  SRR4302547.3640229/94‑1 (MQ=60)

                     CGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGG                                                                         >  SRR4302547.3644531/1‑94 (MQ=60)

                     CGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGG                                                                         >  SRR4302547.6589845/1‑94 (MQ=60)

                      GTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGT                                                                        <  SRR4302547.3804065/94‑1 (MQ=60)

                        CTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCG                                                                      >  SRR4302547.5512034/1‑94 (MQ=60)

                         TGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGC                                                                     <  SRR4302547.3320545/94‑1 (MQ=60)

                          GCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCG                                                                    >  SRR4302547.3551432/1‑94 (MQ=60)

                             TACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATA                                                                 <  SRR4302547.2805025/94‑1 (MQ=60)

                             TACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATA                                                                 <  SRR4302547.2246285/94‑1 (MQ=60)

                              AACGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAA                                                                <  SRR4302547.3330482/94‑1 (MQ=60)

                                  CCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGGAATAAGCCA                                                            <  SRR4302547.6674929/94‑1 (MQ=60)

                                   CACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCAT                                                           >  SRR4302547.3335432/1‑94 (MQ=60)

                                   CACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCAT                                                           >  SRR4302547.2219668/1‑94 (MQ=60)

                                    ACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATA                                                          <  SRR4302547.1258830/94‑1 (MQ=60)

                                    ACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATA                                                          >  SRR4302547.5858413/1‑94 (MQ=60)

                                    ACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATA                                                          >  SRR4302547.3204566/1‑94 (MQ=60)

                                     CAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAG                                                         >  SRR4302547.5565399/1‑94 (MQ=60)

                                     CAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAG                                                         <  SRR4302547.3164350/94‑1 (MQ=60)

                                     CAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAG                                                         <  SRR4302547.4144797/94‑1 (MQ=60)

                                     CAGCAGTAATCCATATCATACTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAG                                                         >  SRR4302547.6635428/1‑94 (MQ=60)

                                      AGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGC                                                        >  SRR4302547.3317956/1‑94 (MQ=60)

                                      AGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGC                                                        >  SRR4302547.4423410/1‑94 (MQ=60)

                                       GCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCT                                                       >  SRR4302547.5399325/1‑94 (MQ=60)

                                       GCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCT                                                       <  SRR4302547.5224255/94‑1 (MQ=60)

                                        CAGTAATCCATATCATATTTACGAGACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTG                                                      <  SRR4302547.6655941/94‑1 (MQ=60)

                                        CAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTG                                                      >  SRR4302547.3087915/1‑94 (MQ=60)

                                        CAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTG                                                      >  SRR4302547.2130966/1‑94 (MQ=60)

                                         tatcttattaagaatgaggtatcagctatggcttAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGA                                                     >  SRR4302547.3938059/35‑94 (MQ=60)

                                         tatcttattaagaatgaggtatcagctatggcttAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGA                                                     >  SRR4302547.3938060/35‑94 (MQ=60)

                                         AGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGA                                                     <  SRR4302547.4200381/94‑1 (MQ=60)

                                           TAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATA                                                   <  SRR4302547.1456990/94‑1 (MQ=60)

                                           TAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATA                                                   <  SRR4302547.1462990/94‑1 (MQ=60)

                                            AATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAC                                                  >  SRR4302547.2337477/1‑94 (MQ=60)

                                             ATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACC                                                 >  SRR4302547.2331494/1‑94 (MQ=60)

                                              TCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCT                                                <  SRR4302547.3818157/94‑1 (MQ=60)

                                              TCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCT                                                >  SRR4302547.544607/1‑94 (MQ=60)

                                               CCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTC                                               >  SRR4302547.2267587/1‑94 (MQ=60)

                                               CCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTC                                               >  SRR4302547.3311721/1‑94 (MQ=60)

                                               CCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTC                                               <  SRR4302547.1296162/94‑1 (MQ=60)

                                               CCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTC                                               <  SRR4302547.3499730/94‑1 (MQ=60)

                                               CCATATCATATTTACGAAACACAGATGAAGCGCGAGGAATAGAGAGGGCCAGTTCACCTAAAGGATGGTCGCGATAAGCCATAGCTGATACCTC                                               >  SRR4302547.2432131/1‑94 (MQ=60)

                                                CATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCA                                              <  SRR4302547.4658328/94‑1 (MQ=60)

                                                CATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCA                                              <  SRR4302547.5857228/94‑1 (MQ=60)

                                                 ATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAT                                             >  SRR4302547.3878823/1‑94 (MQ=60)

                                                  TATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATT                                            >  SRR4302547.5938579/1‑94 (MQ=60)

                                                   ATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTC                                           <  SRR4302547.5226683/94‑1 (MQ=60)

                                                   ATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTC                                           >  SRR4302547.3972974/1‑94 (MQ=60)

                                                   ATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTC                                           <  SRR4302547.5567671/94‑1 (MQ=60)

                                                   ATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTC                                           <  SRR4302547.847214/94‑1 (MQ=60)

                                                    TCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCT                                          >  SRR4302547.6334170/1‑94 (MQ=60)

                                                    TCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCT                                          <  SRR4302547.268017/94‑1 (MQ=60)

                                                    TCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCT                                          >  SRR4302547.3429237/1‑94 (MQ=60)

                                                    TCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCT                                          <  SRR4302547.4832313/94‑1 (MQ=60)

                                                     CATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTT                                         >  SRR4302547.6480945/1‑94 (MQ=60)

                                                     CATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTT                                         >  SRR4302547.598860/1‑94 (MQ=60)

                                                      ATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTA                                        >  SRR4302547.5445225/1‑94 (MQ=60)

                                                        ATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAAT                                      >  SRR4302547.6057840/1‑94 (MQ=60)

                                                        ATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAAT                                      <  SRR4302547.6214168/94‑1 (MQ=60)

                                                          TTTCGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAA                                    >  SRR4302547.330246/1‑94 (MQ=60)

                                                          TTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAA                                    <  SRR4302547.6154070/94‑1 (MQ=60)

                                                          TTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAA                                    <  SRR4302547.4570770/94‑1 (MQ=60)

                                                          TTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAA                                    <  SRR4302547.6426176/94‑1 (MQ=60)

                                                             CGAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGAT                                 <  SRR4302547.3269147/94‑1 (MQ=60)

                                                              GAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATA                                <  SRR4302547.478795/94‑1 (MQ=60)

                                                              GAAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATA                                >  SRR4302547.3957800/1‑94 (MQ=60)

                                                               AAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAA                               >  SRR4302547.5692206/1‑94 (MQ=60)

                                                               AAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAA                               <  SRR4302547.6256213/94‑1 (MQ=60)

                                                               AAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAA                               <  SRR4302547.2972435/94‑1 (MQ=60)

                                                               AAACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAA                               <  SRR4302547.921985/94‑1 (MQ=60)

                                                                AACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAG                              <  SRR4302547.6274432/94‑1 (MQ=60)

                                                                AACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAG                              <  SRR4302547.1085026/94‑1 (MQ=60)

                                                                AACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAG                              >  SRR4302547.4893337/1‑94 (MQ=60)

                                                                AACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAG                              <  SRR4302547.2177845/94‑1 (MQ=60)

                                                                 ACAGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGA                             <  SRR4302547.3816027/94‑1 (MQ=60)

                                                                   AGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG                           >  SRR4302547.5640802/1‑94 (MQ=60)

                                                                   AGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG                           >  SRR4302547.6518495/1‑94 (MQ=60)

                                                                   AGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG                           >  SRR4302547.4742422/1‑94 (MQ=60)

                                                                   AGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG                           >  SRR4302547.3444705/1‑94 (MQ=60)

                                                                   AGAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG                           <  SRR4302547.614008/94‑1 (MQ=60)

                                                                    GAGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGT                          >  SRR4302547.2928598/1‑94 (MQ=60)

                                                                    GAGCTGAAGCGCGAGAAATAGAGAGTGCCACTTCACCTAAAGGTGGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGT                          <  SRR4302547.205057/94‑1 (MQ=60)

                                                                     AGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTA                         <  SRR4302547.5348151/94‑1 (MQ=60)

                                                                     AGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTA                         >  SRR4302547.5492476/1‑94 (MQ=60)

                                                                     AGCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTA                         >  SRR4302547.3761100/1‑94 (MQ=60)

                                                                      GCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTAT                        <  SRR4302547.802368/94‑1 (MQ=60)

                                                                      GCTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTAT                        <  SRR4302547.70784/94‑1 (MQ=60)

                                                                       CTGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATT                       <  SRR4302547.5437057/94‑1 (MQ=60)

                                                                        TGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTT                      <  SRR4302547.1312005/94‑1 (MQ=60)

                                                                        TGAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTT                      <  SRR4302547.4098222/94‑1 (MQ=60)

                                                                         GAAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTT                     <  SRR4302547.739056/94‑1 (MQ=60)

                                                                          AAGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTA                    <  SRR4302547.4802299/94‑1 (MQ=60)

                                                                           AGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAA                   <  SRR4302547.4174465/94‑1 (MQ=60)

                                                                           AGCGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAA                   >  SRR4302547.2172590/1‑94 (MQ=60)

                                                                             CGCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAAT                 <  SRR4302547.5701021/94‑1 (MQ=60)

                                                                              GCGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATG                <  SRR4302547.6486132/94‑1 (MQ=60)

                                                                               CGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGC               >  SRR4302547.2321507/1‑94 (MQ=60)

                                                                               CGAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGC               <  SRR4302547.1624206/94‑1 (MQ=60)

                                                                                GAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCA              <  SRR4302547.2508942/94‑1 (MQ=60)

                                                                                GAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCA              >  SRR4302547.4324130/1‑94 (MQ=60)

                                                                                GAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCA              <  SRR4302547.2038466/94‑1 (MQ=60)

                                                                                GAGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCA              <  SRR4302547.2019478/94‑1 (MQ=60)

                                                                                 AGGAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCAT             >  SRR4302547.6133779/1‑94 (MQ=60)

                                                                                   GAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCT           <  SRR4302547.323512/94‑1 (MQ=60)

                                                                                   GAATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCT           <  SRR4302547.5068013/94‑1 (MQ=60)

                                                                                    AATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTT          <  SRR4302547.4496089/94‑1 (MQ=60)

                                                                                     ATAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTT         <  SRR4302547.2540957/94‑1 (MQ=60)

                                                                                      TAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTA        <  SRR4302547.902675/94‑1 (MQ=60)

                                                                                      TAGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTA        >  SRR4302547.742741/1‑94 (MQ=60)

                                                                                      TAGAGAGTGCCAGTTCACCTAAAGGTTGCTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTA        <  SRR4302547.1794342/94‑1 (MQ=60)

                                                                                       AGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAA       <  SRR4302547.2891260/94‑1 (MQ=60)

                                                                                       AGAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAA       >  SRR4302547.4964979/1‑94 (MQ=60)

                                                                                        GAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAG      >  SRR4302547.4994401/1‑94 (MQ=60)

                                                                                        GAGAGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAG      <  SRR4302547.6600369/94‑1 (MQ=60)

                                                                                           AGTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCA   <  SRR4302547.5308277/94‑1 (MQ=60)

                                                                                            GTGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAA  >  SRR4302547.3102673/1‑94 (MQ=60)

ACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAA  >  NC_000913/4429433‑4429618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`