Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,478,527 Δ1 bp intergenic (+36/+23) yjgN → / ← valS hypothetical protein/valine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,478,5270G.99.5% 985.4 / ‑4.1 209intergenic (+36/+23)yjgN/valShypothetical protein/valine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (122/86);  total (122/87)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

AGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCA  >  NC_000913/4478437‑4478614
                                                                                          |                                                                                       
    ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGGCTT‑CGGTTtcat                                                                                >  1:5754827/1‑93 (MQ=255)
     tcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatc                                                                               >  1:3252925/1‑93 (MQ=255)
      cttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatca                                                                              >  2:3141841/1‑93 (MQ=255)
      cttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatca                                                                              <  2:3467914/93‑1 (MQ=255)
       ttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCACTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAc                                                                             >  1:3011201/1‑93 (MQ=255)
        tAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACt                                                                            <  2:1524975/93‑1 (MQ=255)
         aGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtg                                                                           >  2:5982889/1‑93 (MQ=255)
           ccAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgtg                                                                         >  1:6128033/1‑93 (MQ=255)
           ccAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgtg                                                                         <  2:1370140/93‑1 (MQ=255)
           ccAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgtg                                                                         >  1:2040309/1‑93 (MQ=255)
            cAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTgtgt                                                                        >  2:3902474/1‑93 (MQ=255)
              gTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGttt                                                                      >  1:2152542/1‑93 (MQ=255)
              gTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCCT‑CGTTTTCATCACTGTGttt                                                                      >  1:2367899/1‑93 (MQ=255)
                 tttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGAAGCTGCGGCC‑TCCGTTTTTACCACCGTGctttta                                                                   >  1:80522/1‑91 (MQ=255)
                 tttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACCGA‑GCATGCTCCGGCCCT‑CGTTTTCCTCACTGTGGTTTgt                                                                   >  2:42358/1‑92 (MQ=255)
                  ttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGAt                                                                  <  1:5698015/93‑1 (MQ=255)
                   tCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGAtt                                                                 >  2:3286806/1‑93 (MQ=255)
                      cGGGTAGTTGTGCCTTCTTTCCCATTTCGGTAAGAGAT‑AAAAAGGGCCGGA‑GCATGCTCTGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACa                                                              <  2:4569903/93‑1 (MQ=255)
                       ggggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                                             <  2:5755625/93‑1 (MQ=255)
                       ggggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                                             >  2:5312388/1‑93 (MQ=255)
                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                                            >  1:1978462/1‑93 (MQ=255)
                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                                            <  2:2995086/93‑1 (MQ=255)
                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                                            <  1:4010545/93‑1 (MQ=255)
                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                                            >  2:1838190/1‑93 (MQ=255)
                         ggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcg                                                           >  2:5017749/1‑93 (MQ=255)
                          gAGTTATGCCTTCTTTCCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                                                          <  2:5589828/93‑1 (MQ=255)
                          gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                                                          >  1:3856917/1‑93 (MQ=255)
                          gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                                                          <  2:645875/93‑1 (MQ=255)
                            gTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcgg                                                        <  2:3588703/93‑1 (MQ=255)
                               aTGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGa                                                     <  2:2088679/93‑1 (MQ=255)
                               aTGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGa                                                     >  1:2413961/1‑93 (MQ=255)
                                 gctttcttTCCAATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATa                                                   <  1:5593551/90‑1 (MQ=255)
                                 gCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATa                                                   <  1:5228294/93‑1 (MQ=255)
                                 gCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATa                                                   >  1:6635873/1‑93 (MQ=255)
                                 gCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATa                                                   >  1:1191425/1‑93 (MQ=255)
                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTTATCACTGTGTTTTTATTACAGCGCGGCGATaa                                                  >  2:3749913/1‑93 (MQ=255)
                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATaa                                                  >  1:6008575/1‑93 (MQ=255)
                                   cttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                                                 <  1:1930720/93‑1 (MQ=255)
                                   cttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAACACTGTGGTTTGATTACAGCGCGGCGATCAc                                                 >  1:4827113/1‑93 (MQ=255)
                                      cttTCCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGc                                              <  1:4515789/93‑1 (MQ=255)
                                      cttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGc                                              <  2:862425/93‑1 (MQ=255)
                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGcc                                             >  2:3042105/1‑93 (MQ=255)
                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGcc                                             <  1:3305888/93‑1 (MQ=255)
                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGcc                                             <  2:1341262/93‑1 (MQ=255)
                                        ttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                                            <  2:406884/93‑1 (MQ=255)
                                          aCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgc                                          <  1:2967103/93‑1 (MQ=255)
                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgct                                         >  2:6247578/1‑93 (MQ=255)
                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgct                                         >  1:442541/1‑93 (MQ=255)
                                            cATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgctg                                        >  1:638211/1‑93 (MQ=255)
                                             aTTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGt                                       >  1:1593881/1‑93 (MQ=255)
                                               ttCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTc                                     >  1:5397302/1‑93 (MQ=255)
                                                tCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCa                                    >  2:793647/1‑93 (MQ=255)
                                                tCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCa                                    >  1:2189206/1‑93 (MQ=255)
                                                  tGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAt                                  <  1:2721744/93‑1 (MQ=255)
                                                  tGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAt                                  <  2:3110014/93‑1 (MQ=255)
                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCCATc                                 >  1:2855453/1‑93 (MQ=255)
                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc                                 >  1:2859519/1‑93 (MQ=255)
                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc                                 >  1:2794155/1‑93 (MQ=255)
                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc                                 <  2:2219905/93‑1 (MQ=255)
                                                    tAAGAGAT‑AAAAAAGGCCGG‑GGCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTTAATCa                                >  2:153727/1‑93 (MQ=255)
                                                       gagaT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtt                             <  1:5758346/93‑1 (MQ=255)
                                                            taaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgc                         >  1:4286074/2‑93 (MQ=255)
                                                            taaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgc                         >  2:5805931/2‑93 (MQ=255)
                                                             aaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATTACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgct                        >  1:3082017/1‑93 (MQ=255)
                                                              aaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctt                       <  1:50493/93‑1 (MQ=255)
                                                               aaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgcttt                      >  1:3870789/1‑93 (MQ=255)
                                                               aaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgcttt                      >  2:1450116/1‑93 (MQ=255)
                                                               aaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgcttt                      <  1:3396744/93‑1 (MQ=255)
                                                               aaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGttacgcttt                      <  1:5520622/93‑1 (MQ=255)
                                                                 aaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAATAGCTTGCTGTTCAATTAGtttcgctttcg                    >  1:641204/1‑93 (MQ=255)
                                                                  aGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctttcgc                   >  1:1016365/1‑93 (MQ=255)
                                                                  aGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctttcgc                   >  2:2349134/1‑93 (MQ=255)
                                                                   ggCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTttcgctttcgct                  <  2:1640903/93‑1 (MQ=255)
                                                                   ggCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTttcgctttcgct                  >  1:1706936/1‑93 (MQ=255)
                                                                   ggCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTttcgctttcgct                  >  1:2774090/1‑93 (MQ=255)
                                                                   ggCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTttcgctttcgct                  >  2:3988872/1‑93 (MQ=255)
                                                                   ggCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTttcgctttcgct                  <  1:4979616/93‑1 (MQ=255)
                                                                       cggagcatgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTcc               <  2:682083/89‑1 (MQ=255)
                                                                        ggagcatgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCg              >  2:1588815/4‑93 (MQ=255)
                                                                        ggagcatgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCg              >  2:1814970/4‑93 (MQ=255)
                                                                        ggagcatgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCg              >  1:1331985/4‑93 (MQ=255)
                                                                        ggagcatgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCg              <  2:3929901/90‑1 (MQ=255)
                                                                          agcatgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCa            >  2:2147238/2‑93 (MQ=255)
                                                                           gcatgcTCCGTCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCAt           <  2:5956274/93‑1 (MQ=255)
                                                                           gcatgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCAt           <  2:6039449/93‑1 (MQ=255)
                                                                            catgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATa          <  1:754365/93‑1 (MQ=255)
                                                                            catgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATa          >  1:5152983/1‑93 (MQ=255)
                                                                            catgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATa          >  2:5418614/1‑93 (MQ=255)
                                                                             atgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAg         >  2:5740871/1‑93 (MQ=255)
                                                                              tgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGTATAGc        <  1:4327774/93‑1 (MQ=255)
                                                                              tgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGc        >  2:1194320/1‑93 (MQ=255)
                                                                              tgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGc        <  2:2277905/93‑1 (MQ=255)
                                                                              tgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGc        >  1:696874/1‑93 (MQ=255)
                                                                              tgcTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGc        <  1:1040616/93‑1 (MQ=255)
                                                                                 tCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCtt     >  2:4314042/1‑93 (MQ=255)
                                                                                  ccGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTc    >  2:1041655/1‑93 (MQ=255)
                                                                                  ccGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTc    >  1:3581774/1‑93 (MQ=255)
                                                                                   cGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTcc   >  2:124373/1‑93 (MQ=255)
                                                                                   cGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTcc   <  2:635586/93‑1 (MQ=255)
                                                                                   cGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTcc   <  2:2008/93‑1 (MQ=255)
                                                                                          |                                                                                       
AGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCA  >  NC_000913/4478437‑4478614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  >  NC_000913/4478432‑4478620
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GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTC                                                                                               <  SRR4302547.5492554/94‑1 (MQ=60)
  TAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                                            >  SRR4302547.4504691/1‑94 (MQ=60)
  TAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                                            <  SRR4302547.2713155/94‑1 (MQ=60)
   AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                                           >  SRR4302547.2129576/1‑94 (MQ=60)
     AGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTT                                                                                         >  SRR4302547.727141/1‑94 (MQ=60)
      GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                                        >  SRR4302547.6534939/1‑94 (MQ=60)
      GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                                        <  SRR4302547.5575838/94‑1 (MQ=60)
      GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                                        >  SRR4302547.699752/1‑94 (MQ=60)
         TTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATC                                                                                     >  SRR4302547.3317919/1‑94 (MQ=60)
         TTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATC                                                                                     >  SRR4302547.5064394/1‑94 (MQ=60)
           CTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCACTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAC                                                                                   >  SRR4302547.3071073/1‑94 (MQ=60)
              AGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGTCGGA‑GCATGCTCCGGCCTT‑CGTTTTCCTCACTGT                                                                                >  SRR4302547.172336/1‑94 (MQ=60)
              AGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGT                                                                                >  SRR4302547.207674/1‑94 (MQ=60)
               GCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG                                                                               >  SRR4302547.6255154/1‑94 (MQ=60)
               GCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG                                                                               >  SRR4302547.2080997/1‑94 (MQ=60)
                 CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTT                                                                             >  SRR4302547.3841356/1‑94 (MQ=60)
                 CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTT                                                                             >  SRR4302547.5634885/1‑94 (MQ=60)
                  AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                                            >  SRR4302547.2195520/1‑94 (MQ=60)
                  AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCCT‑CGTTTTCATCACTGTGTTT                                                                            >  SRR4302547.2415894/1‑94 (MQ=60)
                       TTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATT                                                                       <  SRR4302547.5815708/94‑1 (MQ=60)
                        TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTA                                                                      >  SRR4302547.4550318/1‑94 (MQ=60)
                        TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTA                                                                      >  SRR4302547.3111337/1‑94 (MQ=60)
                         CACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTAC                                                                     >  SRR4302547.6094171/1‑94 (MQ=60)
                         CACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTAC                                                                     >  SRR4302547.335599/1‑94 (MQ=60)
                            GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGC                                                                  <  SRR4302547.4053802/94‑1 (MQ=60)
                            GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGC                                                                  >  SRR4302547.2017739/1‑94 (MQ=60)
                             GGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCG                                                                 >  SRR4302547.3375045/1‑94 (MQ=60)
                             GGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCG                                                                 <  SRR4302547.4092063/94‑1 (MQ=60)
                              GGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGC                                                                >  SRR4302547.1130251/1‑94 (MQ=60)
                              GGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGC                                                                >  SRR4302547.3935415/1‑94 (MQ=60)
                               GATTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCG                                                               <  SRR4302547.1230175/94‑1 (MQ=60)
                               GAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCG                                                               >  SRR4302547.3050599/1‑94 (MQ=60)
                                 GTGATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGC                                                             <  SRR4302547.2836175/94‑1 (MQ=60)
                                   TATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGA                                                           >  SRR4302547.6194252/1‑94 (MQ=60)
                                   TATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGA                                                           >  SRR4302547.2462837/1‑94 (MQ=60)
                                    ATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGAT                                                          >  SRR4302547.2215160/1‑94 (MQ=60)
                                     TGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATA                                                         >  SRR4302547.1215468/1‑94 (MQ=60)
                                     TGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATA                                                         >  SRR4302547.6774001/1‑94 (MQ=60)
                                     TGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATA                                                         >  SRR4302547.449712/1‑94 (MQ=60)
                                      GCTTTCTTTCCAATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                                        <  SRR4302547.5708939/94‑1 (MQ=60)
                                      GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                                        >  SRR4302547.6133283/1‑94 (MQ=60)
                                      GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                                        >  SRR4302547.2810327/1‑94 (MQ=60)
                                      GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                                        <  SRR4302547.693794/94‑1 (MQ=60)
                                      GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                                        <  SRR4302547.5335918/94‑1 (MQ=60)
                                       CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                                       >  SRR4302547.4409361/1‑94 (MQ=60)
                                       CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAACACTGTGGTTTGATTACAGCGCGGCGATCAC                                                       >  SRR4302547.4927194/1‑94 (MQ=60)
                                        CTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACA                                                      <  SRR4302547.1968995/94‑1 (MQ=60)
                                        CTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACA                                                      >  SRR4302547.636807/1‑94 (MQ=60)
                                          TCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGC                                                    >  SRR4302547.4078028/1‑94 (MQ=60)
                                           CTTTCCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCC                                                   <  SRR4302547.4609090/94‑1 (MQ=60)
                                            TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  <  SRR4302547.3372510/94‑1 (MQ=60)
                                            TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  >  SRR4302547.2252847/1‑94 (MQ=60)
                                             TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTG                                                 >  SRR4302547.3940265/1‑94 (MQ=60)
                                               ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                                               <  SRR4302547.3026152/94‑1 (MQ=60)
                                               ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                                               >  SRR4302547.450784/1‑94 (MQ=60)
                                                CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                                              <  SRR4302547.5876451/94‑1 (MQ=60)
                                                CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                                              >  SRR4302547.649995/1‑94 (MQ=60)
                                                 CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                                             <  SRR4302547.4096975/94‑1 (MQ=60)
                                                 CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                                             <  SRR4302547.1571793/94‑1 (MQ=60)
                                                 CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                                             >  SRR4302547.1625527/1‑94 (MQ=60)
                                                 CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                                             >  SRR4302547.2913839/1‑94 (MQ=60)
                                                  ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAAAGCCTGCTGTT                                            >  SRR4302547.2148835/1‑94 (MQ=60)
                                                   TTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTC                                           >  SRR4302547.5508823/1‑94 (MQ=60)
                                                    TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          <  SRR4302547.5308799/94‑1 (MQ=60)
                                                    TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          >  SRR4302547.2233130/1‑94 (MQ=60)
                                                      CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAT                                        <  SRR4302547.2884271/94‑1 (MQ=60)
                                                      CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAT                                        >  SRR4302547.2856200/1‑94 (MQ=60)
                                                       TGTAAGTGAT‑AAAAAAGGCCGTA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                                       <  SRR4302547.3274538/94‑1 (MQ=60)
                                                       TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCCATC                                       >  SRR4302547.2912510/1‑94 (MQ=60)
                                                       TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                                       >  SRR4302547.2850098/1‑94 (MQ=60)
                                                       TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                                       >  SRR4302547.5144009/1‑94 (MQ=60)
                                                       TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                                       <  SRR4302547.2776401/94‑1 (MQ=60)
                                                       TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                                       >  SRR4302547.2916650/1‑94 (MQ=60)
                                                        GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                                      <  SRR4302547.2421047/94‑1 (MQ=60)
                                                           AGAGAT‑AAAAAAGGCCGGA‑GCATGGTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT                                   >  SRR4302547.5376568/1‑94 (MQ=60)
                                                           AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT                                   <  SRR4302547.2314931/94‑1 (MQ=60)
                                                            GAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                                  <  SRR4302547.5836759/94‑1 (MQ=60)
                                                            GAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                                  <  SRR4302547.5877412/94‑1 (MQ=60)
                                                             AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC                                 <  SRR4302547.2709225/94‑1 (MQ=60)
                                                                atAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGC                               >  SRR4302547.4374154/3‑94 (MQ=60)
                                                                 TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATTACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT                              >  SRR4302547.3143202/1‑94 (MQ=60)
                                                                   AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT                            >  SRR4302547.3949578/1‑94 (MQ=60)
                                                                   AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT                            >  SRR4302547.3013589/1‑94 (MQ=60)
                                                                   AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT                            <  SRR4302547.51437/94‑1 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           <  SRR4302547.3465859/94‑1 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTACGCTTTC                           <  SRR4302547.5634585/94‑1 (MQ=60)
                                                                     AAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAATAGCTTGCTGTTCAATTAGTTTCGCTTTCG                          >  SRR4302547.653033/1‑94 (MQ=60)
                                                                      AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC                         >  SRR4302547.1036269/1‑94 (MQ=60)
                                                                      AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC                         >  SRR4302547.1778621/1‑94 (MQ=60)
                                                                       AGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT                        >  SRR4302547.2829656/1‑94 (MQ=60)
                                                                       AGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT                        >  SRR4302547.1740520/1‑94 (MQ=60)
                                                                        GGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTT                       <  SRR4302547.5082605/94‑1 (MQ=60)
                                                                        GGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTT                       >  SRR4302547.364473/1‑94 (MQ=60)
                                                                         GCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTC                      >  SRR4302547.1192400/1‑94 (MQ=60)
                                                                          CCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCC                     <  SRR4302547.5372112/94‑1 (MQ=60)
                                                                          CCGGA‑GCATCCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCC                     <  SRR4302547.75690/94‑1 (MQ=60)
                                                                           CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG                    >  SRR4302547.1358757/1‑94 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   >  SRR4302547.653719/1‑94 (MQ=60)
                                                                                TCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATA                <  SRR4302547.5236565/94‑1 (MQ=60)
                                                                                GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATA                <  SRR4302547.5409134/94‑1 (MQ=60)
                                                                                GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATA                >  SRR4302547.5259108/1‑94 (MQ=60)
                                                                                 CATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAG               <  SRR4302547.768582/94‑1 (MQ=60)
                                                                                 CATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAG               >  SRR4302547.4866030/1‑94 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302547.709755/1‑94 (MQ=60)
                                                                                   TGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGTATAGCC             <  SRR4302547.4416841/94‑1 (MQ=60)
                                                                                   TGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCC             >  SRR4302547.3364555/1‑94 (MQ=60)
                                                                                   TGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCC             <  SRR4302547.1061129/94‑1 (MQ=60)
                                                                                   TGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCC             >  SRR4302547.1038242/1‑94 (MQ=60)
                                                                                    GCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCT            >  SRR4302547.1975106/1‑94 (MQ=60)
                                                                                     CTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTT           >  SRR4302547.995577/1‑94 (MQ=60)
                                                                                      TCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTC          <  SRR4302547.5995867/94‑1 (MQ=60)
                                                                                      TCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTC          >  SRR4302547.3654858/1‑94 (MQ=60)
                                                                                      TCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTC          <  SRR4302547.1744067/94‑1 (MQ=60)
                                                                                      TCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCCTC          >  SRR4302547.5756748/1‑94 (MQ=60)
                                                                                        CGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCA        >  SRR4302547.802275/1‑94 (MQ=60)
                                                                                         GGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAG       >  SRR4302547.594046/1‑94 (MQ=60)
                                                                                          GCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGC      >  SRR4302547.1069743/1‑94 (MQ=60)
                                                                                          GCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGC      >  SRR4302547.168461/1‑94 (MQ=60)
                                                                                           CCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCTTTCCAGCT     >  SRR4302547.4658030/1‑94 (MQ=60)
                                                                                           CCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCT     <  SRR4302547.1076865/94‑1 (MQ=60)
                                                                                           CCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCT     <  SRR4302547.1356416/94‑1 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    >  SRR4302547.1051939/1‑94 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    <  SRR4302547.113504/94‑1 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    <  SRR4302547.1763443/94‑1 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    <  SRR4302547.3598408/94‑1 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    <  SRR4302547.5233499/94‑1 (MQ=60)
                                                                                              TTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTC   >  SRR4302547.3379492/1‑94 (MQ=60)
                                                                                              TTCGTTTTCATCACTGTGTTTTGATTACAGCGCAGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTC   >  SRR4302547.3138848/1‑94 (MQ=60)
                                                                                               TCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  <  SRR4302547.2277649/94‑1 (MQ=60)
                                                                                               TCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  <  SRR4302547.3579917/94‑1 (MQ=60)
                                                                                               |                                                                                             
GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  >  NC_000913/4478432‑4478620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑