Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F3 I160 R1 220 104.1 6051714 95.9% 5803593 84.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,057,873 C→G W418C (TGG→TGC)  yihO ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,057,8730CG100.0% 497.4 / NA 130W418C (TGG→TGC) yihOMFS transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (63/67);  total (63/67)

Only 100 of 130 total aligned reads displayed.

TTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAA  >  NZ_CP009273/4057786‑4057958
                                                                                       |                                                                                     
ttCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCa                                                                                     <  1:1150480/90‑1 (MQ=255)
ttCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCa                                                                                     <  1:2751322/90‑1 (MQ=255)
ttCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCa                                                                                     <  1:2743004/90‑1 (MQ=255)
ttCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCa                                                                                     <  2:2724260/90‑1 (MQ=255)
    tCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATa                                                                                 >  1:636185/1‑90 (MQ=255)
       ataatCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATaaaa                                                                              >  1:1812312/1‑90 (MQ=255)
          atCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATg                                                                           <  2:2533542/90‑1 (MQ=255)
             aaTGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGc                                                                        >  1:920424/1‑90 (MQ=255)
             aaTGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGc                                                                        <  2:1297064/90‑1 (MQ=255)
             aaTGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGc                                                                        <  2:1807225/90‑1 (MQ=255)
               tGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCGATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTg                                                                      <  2:2114409/90‑1 (MQ=255)
               tGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTg                                                                      >  1:3021333/1‑90 (MQ=255)
               tGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTg                                                                      <  2:2082422/90‑1 (MQ=255)
               tGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTg                                                                      <  1:2330843/90‑1 (MQ=255)
               tGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTg                                                                      >  1:1948385/1‑90 (MQ=255)
                    aCCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCa                                                                 <  2:2361975/90‑1 (MQ=255)
                    aCCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCa                                                                 <  1:147271/90‑1 (MQ=255)
                    aCCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCa                                                                 <  1:1594466/90‑1 (MQ=255)
                     ccGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                                >  2:743467/1‑90 (MQ=255)
                     ccGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                                <  1:1228957/90‑1 (MQ=255)
                     ccGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                                <  1:1390479/90‑1 (MQ=255)
                     ccGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                                <  1:2535020/90‑1 (MQ=255)
                      cGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaaa                                                               <  1:243373/90‑1 (MQ=255)
                      cGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaaa                                                               <  2:140225/90‑1 (MQ=255)
                       gTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAc                                                              <  2:1036544/90‑1 (MQ=255)
                        tttttCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAcc                                                             >  2:257827/1‑90 (MQ=255)
                          tttCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAc                                                              >  1:515264/1‑87 (MQ=255)
                          tttCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAc                                                              <  2:515264/87‑1 (MQ=255)
                               ttGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAAt                                                      >  2:1694316/1‑90 (MQ=255)
                               ttGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAAt                                                      <  2:3021333/90‑1 (MQ=255)
                               ttGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAAt                                                      >  2:624848/1‑90 (MQ=255)
                               ttGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAAt                                                      >  2:2417928/1‑90 (MQ=255)
                                 gAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGt                                                    <  2:1812312/90‑1 (MQ=255)
                                 gAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGt                                                    >  2:2988592/1‑90 (MQ=255)
                                 gAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGAAGCAAACCCTGTAATGt                                                    >  1:1608396/1‑90 (MQ=255)
                                    tgtgtAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGc                                                 >  2:2456304/1‑90 (MQ=255)
                                     gtgtAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCa                                                >  2:2061388/1‑90 (MQ=255)
                                     gtgtAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCa                                                >  1:974887/1‑90 (MQ=255)
                                     gtgtAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCa                                                >  1:2434419/1‑90 (MQ=255)
                                      tgtAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCAt                                               <  2:1374100/90‑1 (MQ=255)
                                       gtAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATc                                              >  1:863144/1‑90 (MQ=255)
                                           aaGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCtt                                          <  1:916129/90‑1 (MQ=255)
                                           aaGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCtt                                          >  1:1649961/1‑90 (MQ=255)
                                             gAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACGAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTg                                        >  2:59656/1‑90 (MQ=255)
                                             gAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTg                                        >  1:2509885/1‑90 (MQ=255)
                                             gAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTg                                        >  2:1659698/1‑90 (MQ=255)
                                              aaTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgc                                       <  2:828420/90‑1 (MQ=255)
                                              aaTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgc                                       >  1:1497631/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                                >  1:428822/1‑64 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                                <  2:428822/64‑1 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCAt                                               <  1:1145991/81‑1 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCAt                                               >  2:1145991/1‑81 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCtt                                          >  2:2014595/1‑86 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCtt                                          <  1:2014595/86‑1 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      <  1:2983314/90‑1 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  1:78701/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  2:2983314/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  2:1582918/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  1:78038/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  2:635984/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  1:2389667/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  1:2567765/1‑90 (MQ=255)
                                               aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  1:353953/1‑90 (MQ=255)
                                               aTCCCATCATCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGAt                                                                                  >  1:540704/1‑46 (MQ=38)
                                                tCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcgc                                     <  2:838701/90‑1 (MQ=255)
                                                tCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcgc                                     <  2:1839523/90‑1 (MQ=255)
                                                tCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      <  1:2193504/89‑1 (MQ=255)
                                                tCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                      >  2:2193504/1‑89 (MQ=255)
                                                  ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa                                   <  2:2266183/90‑1 (MQ=255)
                                                  ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa                                   <  1:1659698/90‑1 (MQ=255)
                                                  ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa                                   <  2:36884/90‑1 (MQ=255)
                                                  ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa                                   <  1:22154/90‑1 (MQ=255)
                                                  ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa                                   <  1:805397/90‑1 (MQ=255)
                                                       gTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTg                              <  1:2455723/90‑1 (MQ=255)
                                                       gTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTg                              <  2:1948385/90‑1 (MQ=255)
                                                        tCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTgg                             <  1:2988592/90‑1 (MQ=255)
                                                           aTGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGaa                          >  2:1936223/1‑90 (MQ=255)
                                                            tGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAAc                         <  2:460720/90‑1 (MQ=255)
                                                             gcagcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACa                        >  2:1606688/1‑90 (MQ=255)
                                                              cagcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACAt                       >  2:1798833/1‑90 (MQ=255)
                                                                gcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcgc                                     <  2:2200525/74‑1 (MQ=255)
                                                                gcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcgc                                     >  1:2200525/1‑74 (MQ=255)
                                                                gcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATaa                     >  2:1033193/1‑90 (MQ=255)
                                                                gcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATaa                     >  1:2609681/1‑90 (MQ=255)
                                                                gcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATaa                     <  1:2832160/90‑1 (MQ=255)
                                                                  aataatTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAAcc                   <  1:1936223/90‑1 (MQ=255)
                                                                     aatTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAAt                >  1:186370/1‑90 (MQ=255)
                                                                        tGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTg             >  2:241345/1‑90 (MQ=255)
                                                                          ccAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCg           >  2:230891/1‑90 (MQ=255)
                                                                          ccAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCg           >  1:1280885/1‑90 (MQ=255)
                                                                           cAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGt          >  1:2767861/1‑90 (MQ=255)
                                                                            aGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc         <  2:2590114/90‑1 (MQ=255)
                                                                            aGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc         <  2:2264917/90‑1 (MQ=255)
                                                                            aGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc         <  1:1223541/90‑1 (MQ=255)
                                                                            aGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc         <  1:332066/90‑1 (MQ=255)
                                                                             gTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa                                   >  1:2646988/1‑63 (MQ=255)
                                                                             gTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCa        >  2:2884053/1‑90 (MQ=255)
                                                                             gTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCa        >  2:2650079/1‑90 (MQ=255)
                                                                             gTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCa        >  2:219800/1‑90 (MQ=255)
                                                                                   aaGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATaa  <  1:359726/90‑1 (MQ=255)
                                                                                       |                                                                                     
TTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAA  >  NZ_CP009273/4057786‑4057958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCCTGGCAA  >  NZ_CP009273/4057774‑4057968
                                                                                                   |                                                                                               
GCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGG                                                                                                 <  SRR3722030.386373/100‑1 (MQ=60)
 CGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGC                                                                                                <  SRR3722030.24408/100‑1 (MQ=60)
      GTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATA                                                                                           >  SRR3722030.646068/1‑100 (MQ=60)
       TTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAA                                                                                          <  SRR3722030.1820952/100‑1 (MQ=60)
       TTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAA                                                                                          <  SRR3722030.3040496/100‑1 (MQ=60)
         GATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAA                                                                                        >  SRR3722030.1842931/1‑100 (MQ=60)
            TTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATG                                                                                     <  SRR3722030.1167617/100‑1 (MQ=60)
            TTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATG                                                                                     <  SRR3722030.2493314/100‑1 (MQ=60)
            TTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATG                                                                                     <  SRR3722030.2793664/100‑1 (MQ=60)
            TTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATG                                                                                     <  SRR3722030.2802130/100‑1 (MQ=60)
               CTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGC                                                                                  >  SRR3722030.934527/1‑100 (MQ=60)
               CTCAATAATCAATGCAAACCGTTTTTAATTGAGTGTGTAAAAGAATCCCACCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGC                                                                                  <  SRR3722030.316045/100‑1 (MQ=60)
                 CAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTG                                                                                >  SRR3722030.3076970/1‑100 (MQ=60)
                 CAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTG                                                                                >  SRR3722030.1982374/1‑100 (MQ=60)
                           TGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCC                                                                      <  SRR3722030.2373166/100‑1 (MQ=60)
                            GCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCT                                                                     >  SRR3722030.523168/1‑100 (MQ=60)
                                ACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAA                                                                 <  SRR3722030.1620324/100‑1 (MQ=60)
                                ACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAA                                                                 <  SRR3722030.149399/100‑1 (MQ=60)
                                 CCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATctgtctcttatacacatctgac                                                                >  SRR3722030.2812136/1‑78 (MQ=60)
                                 CCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAAT                                                                <  SRR3722030.1412320/100‑1 (MQ=60)
                                 CCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAAT                                                                <  SRR3722030.1247069/100‑1 (MQ=60)
                                 CCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAAT                                                                <  SRR3722030.2581442/100‑1 (MQ=60)
                                  CGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATG                                                               <  SRR3722030.246879/100‑1 (MQ=60)
                                  CGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATG                                                               <  SRR3722030.1290602/100‑1 (MQ=60)
                                   GTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGT                                                              >  SRR3722030.2305869/1‑100 (MQ=60)
                                   GTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGT                                                              >  SRR3722030.841139/1‑100 (MQ=60)
                                   GTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGT                                                              <  SRR3722030.987390/100‑1 (MQ=60)
                                   GTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGAAGCAAACCCTGTAATGT                                                              >  SRR3722030.1634482/1‑100 (MQ=60)
                                       TTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCA                                                          >  SRR3722030.2478823/1‑100 (MQ=60)
                                       TTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCA                                                          >  SRR3722030.989728/1‑100 (MQ=60)
                                       TTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCA                                                          >  SRR3722030.196277/1‑100 (MQ=60)
                                         CATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATC                                                        >  SRR3722030.876393/1‑100 (MQ=60)
                                          ATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCG                                                       <  SRR3722030.1511297/100‑1 (MQ=60)
                                           TTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGC                                                      <  SRR3722030.2907658/100‑1 (MQ=60)
                                             GAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTT                                                    >  SRR3722030.1676750/1‑100 (MQ=60)
                                               GTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTG                                                  >  SRR3722030.2555811/1‑100 (MQ=60)
                                                TGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGC                                                 >  SRR3722030.1521654/1‑100 (MQ=60)
                                                 GTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATctgtctcttatacaca                                                >  SRR3722030.435322/1‑84 (MQ=60)
                                                 GTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCG                                                >  SRR3722030.2614856/1‑100 (MQ=60)
                                                 GTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCG                                                >  SRR3722030.359199/1‑100 (MQ=60)
                                                 GTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCG                                                >  SRR3722030.79244/1‑100 (MQ=60)
                                                 GTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCG                                                >  SRR3722030.79921/1‑100 (MQ=60)
                                                 GTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCG                                                >  SRR3722030.2433149/1‑100 (MQ=60)
                                                 GTGTAAAAGAATCCCATCATCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCctgtctcttatacacatctgacgctgccgacgac                                                >  SRR3722030.549048/1‑66 (MQ=60)
                                                  TGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGC                                               <  SRR3722030.1163057/100‑1 (MQ=60)
                                                       AAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGT                                          <  SRR3722030.2050103/100‑1 (MQ=60)
                                                       AAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGT                                          <  SRR3722030.930171/100‑1 (MQ=60)
                                                           ATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATActgtctcttatacacatctgacgctgccgacgacatggccagtgtagatctcg                                      >  SRR3722030.1425196/1‑47 (MQ=60)
                                                           ATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGG                                      <  SRR3722030.2232929/100‑1 (MQ=60)
                                                           ATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGG                                      <  SRR3722030.3038262/100‑1 (MQ=60)
                                                            gtcggcagcgtcagatgtgtataagagacagGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGA                                     <  SRR3722030.59921/69‑1 (MQ=60)
                                                              CCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAAC                                   <  SRR3722030.817786/100‑1 (MQ=60)
                                                              CCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAAC                                   <  SRR3722030.717504/100‑1 (MQ=60)
                                                              CCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAAC                                   <  SRR3722030.1686650/100‑1 (MQ=60)
                                                              CCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAAC                                   <  SRR3722030.22516/100‑1 (MQ=60)
                                                                  CGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGActgtct                               >  SRR3722030.2240096/1‑94 (MQ=60)
                                                                  CGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAA                               >  SRR3722030.2657603/1‑100 (MQ=60)
                                                                   GTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAAC                              <  SRR3722030.2500591/100‑1 (MQ=60)
                                                                    TCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACC                             <  SRR3722030.3043632/100‑1 (MQ=60)
                                                                       ATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAAT                          >  SRR3722030.189049/1‑100 (MQ=60)
                                                                            GCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCG                     <  SRR3722030.2884447/100‑1 (MQ=60)
                                                                            GCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCG                     >  SRR3722030.1300166/1‑100 (MQ=60)
                                                                             CAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGT                    >  SRR3722030.2818983/1‑100 (MQ=60)
                                                                              AATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTC                   <  SRR3722030.1969926/100‑1 (MQ=60)
                                                                              AATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGGAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTC                   >  SRR3722030.2071616/1‑100 (MQ=60)
                                                                               ATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACctgtctcttatacacat                  >  SRR3722030.2695720/1‑83 (MQ=60)
                                                                               ATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCA                  >  SRR3722030.397066/1‑100 (MQ=60)
                                                                                        AGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATTC         <  SRR3722030.1241589/100‑1 (MQ=60)
                                                                                        AGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGC         <  SRR3722030.1663530/100‑1 (MQ=60)
                                                                                        AGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGC         <  SRR3722030.2599923/100‑1 (MQ=60)
                                                                                        AGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGC         <  SRR3722030.277793/100‑1 (MQ=60)
                                                                                        AGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGC         <  SRR3722030.336939/100‑1 (MQ=60)
                                                                                         GTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCctgtctcttatacacatctgacgctgccgacgacatggc        >  SRR3722030.1345747/1‑61 (MQ=60)
                                                                                         GTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCctgtctcttatacacatctga        >  SRR3722030.2418831/1‑79 (MQ=60)
                                                                                         GTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCC        >  SRR3722030.2565307/1‑100 (MQ=60)
                                                                                         GTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCC        >  SRR3722030.1179144/1‑100 (MQ=60)
                                                                                           GCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCCTG      >  SRR3722030.1298233/1‑100 (MQ=60)
                                                                                               AAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCCTGGCAA  <  SRR3722030.365083/100‑1 (MQ=60)
                                                                                                   |                                                                                               
GCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCCTGGCAA  >  NZ_CP009273/4057774‑4057968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑