Notes

Publication: None

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 3 67
2 4 68

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 444 1336
Multiple Base Substitutions 1 1
Deletions 60 255
Insertions 14 32
Mobile Element Insertions 0 0
Amplifications 112 148
Gene Conversions 0 0
Inversions 0 0
Total 631 1772

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 3 0 57.3 3828570 94.4% 3614170 84.4
A1 F1 I2 R2 5 0 59.1 3845041 93.4% 3591268 82.4
A1 F1 I3 R2 24 0 0.0 21072 39.5% 8323 78.9
A1 F1 I5 R2 2 0 20.2 1215144 92.8% 1127653 83.6
A1 F1 I6 R2 4 0 13.9 847578 94.4% 800113 84.1
A1 F2 I74 R1 158 0 13.7 815430 95.7% 780366 86.0
A1 F2 I75 R1 71 0 32.8 2150506 93.3% 2006422 83.2
A1 F2 I76 R1 7 0 11.0 663162 95.7% 634646 85.7
A1 F2 I77 R1 69 0 0.0 0 0.0% 0 0.0
A1 F2 I78 R1 12 0 11.6 697012 95.6% 666343 85.5
A1 F2 I79 R1 9 0 10.0 590080 95.6% 564116 85.0
A1 F2 I80 R1 9 0 12.5 716974 96.4% 691162 86.0
A1 F2 I81 R1 9 0 8.6 496014 96.7% 479645 86.3
A1 F2 I82 R1 8 0 11.7 688212 96.3% 662748 85.4
A1 F2 I83 R1 6 0 17.0 977548 95.8% 936490 84.9
A1 F2 I84 R1 6 0 18.6 1086478 95.6% 1038672 85.6
A1 F2 I85 R1 6 0 9.1 514804 96.7% 497815 86.5
A1 F2 I86 R1 13 0 0.0 0 0.0% 0 0.0
A1 F2 I87 R1 7 0 17.1 1012950 96.1% 973444 85.2
A1 F2 I88 R1 2 0 0.0 21158 10.6% 2242 79.4
A1 F2 I89 R1 5 0 15.7 941600 95.5% 899228 84.7
A1 F2 I90 R1 5 0 19.3 1188686 94.4% 1122119 83.5
A1 F2 I91 R1 6 0 18.5 1088486 95.8% 1042769 84.9
A1 F2 I92 R1 2 0 12.6 736934 95.0% 700087 84.3
A1 F2 I93 R1 5 0 15.0 885740 95.4% 844995 84.1
A1 F2 I94 R1 5 0 13.0 742698 96.2% 714475 85.3
A1 F3 I95 R1 196 0 14.2 808436 96.0% 776098 85.4
A1 F3 I96 R1 172 0 34.7 2005794 96.2% 1929573 85.4
A1 F3 I100 R1 2 0 0.0 13730 17.2% 2361 79.0
A1 F3 I122 R1 193 0 20.9 1228102 94.7% 1163012 84.0
A1 F3 I123 R1 9 0 36.7 2327398 93.5% 2176117 82.5
A1 F3 I124 R1 9 0 28.7 1792628 94.6% 1695826 83.3
A1 F3 I125 R1 8 0 23.4 1420394 94.5% 1342272 83.4
A1 F3 I126 R1 7 0 27.8 1630878 95.5% 1557488 84.0
A1 F3 I127 R1 10 0 20.7 1249536 95.1% 1188308 83.9
A1 F3 I128 R1 7 0 33.6 1999592 94.8% 1895613 83.5
A1 F3 I129 R1 10 0 20.4 1208620 95.3% 1151814 84.1
A1 F3 I130 R1 9 0 27.0 1591488 96.0% 1527828 85.7
A1 F3 I131 R1 7 0 31.9 1885958 94.8% 1787888 84.0
A1 F3 I132 R1 20 0 0.0 0 0.0% 0 0.0
A1 F3 I133 R1 8 0 25.9 1516138 96.2% 1458524 85.4
A1 F3 I134 R1 6 0 21.3 1193788 96.3% 1149617 85.7
A1 F3 I135 R1 12 0 14.9 912542 96.9% 884253 86.0
A1 F3 I136 R1 8 0 18.0 1078482 95.8% 1033185 84.7
A1 F3 I137 R1 8 0 51.7 3166574 94.6% 2995579 84.8
A1 F3 I138 R1 8 0 27.5 1620068 96.7% 1566605 85.6
A1 F3 I139 R1 10 0 45.6 2930156 95.5% 2798298 83.8
A1 F3 I140 R1 11 0 50.8 3223530 93.3% 3007553 83.7
A1 F3 I141 R1 10 0 17.9 1193536 95.8% 1143407 84.9
A1 F3 I142 R1 10 0 30.9 1778688 96.4% 1714655 84.8
A1 F3 I143 R1 8 0 24.1 1400000 95.9% 1342600 84.8
A1 F3 I144 R1 10 0 24.9 1432174 96.4% 1380615 85.3
A1 F3 I146 R1 8 0 43.8 2728252 95.4% 2602752 84.7
A1 F3 I147 R1 10 0 27.9 1981380 94.3% 1868441 83.6
A1 F3 I148 R1 8 0 25.7 1750778 89.7% 1570447 79.4
A1 F3 I149 R1 8 0 65.0 3813846 96.1% 3665106 85.1
A1 F3 I150 R1 9 0 47.7 3300572 95.9% 3165248 84.9
A1 F3 I151 R1 8 0 20.9 1215326 95.5% 1160636 84.4
A1 F3 I152 R1 8 0 44.1 2758600 95.6% 2637221 84.7
A1 F3 I153 R1 9 0 31.4 1878488 94.6% 1777049 83.6
A1 F3 I154 R1 12 0 36.3 2142882 95.4% 2044309 85.1
A1 F3 I155 R1 6 0 17.6 1031254 95.6% 985878 84.7
A1 F3 I156 R1 18 0 0.0 0 0.0% 0 0.0
A1 F3 I157 R1 5 0 22.3 1326252 93.5% 1240045 82.9
A1 F3 I158 R1 3 0 18.7 1109348 93.0% 1031693 82.7
A1 F3 I159 R1 206 0 0.0 0 0.0% 0 0.0
A1 F3 I160 R1 220 0 104.1 6051714 95.9% 5803593 84.4
A1 F3 I161 R1 8 0 14.2 829238 94.5% 783629 83.4

Mutation Needle Plot

Mutation Targets

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