breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→G D27E (GAT→GAG folA → type 3 dihydrofolate reductase
RA 50,279 T→C F153L (TTT→CTT)  folA → type 3 dihydrofolate reductase
RA 1,613,723 G→C G116A (GGC→GCC)  marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356976 357573 598 4 [3] [3] 4 [lacA] [lacA]
* * ÷ NZ_CP009273 578897 579033 137 4 [2] [3] 4 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 613493 613561 69 7 [3] [3] 4 [entF] [entF]
* * ÷ NZ_CP009273 982708 982746 39 5 [2] [3] 4 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase
* * ÷ NZ_CP009273 1116572 1116606 35 5 [3] [3] 4 bssS/dinI biofilm formation regulator BssS/DNA damage‑inducible protein I
* * ÷ NZ_CP009273 1205059 1205074 16 18 [0] [0] 31 BW25113_RS06005 phage tail protein
* * ÷ NZ_CP009273 1207580 1207689 110 4 [3] [3] 5 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1748979 1749084 106 4 [2] [2] 4 ydhY/fumD ferredoxin‑like protein/fumarate hydratase FumD
* * ÷ NZ_CP009273 1800499 1800537 39 4 [2] [2] 4 ydiY/pfkB YdiY family protein/6‑phosphofructokinase II
* * ÷ NZ_CP009273 2027336 2027453 118 4 [2] [2] 4 drpB/ompC cell division protein DrpB/porin OmpC
* * ÷ NZ_CP009273 2096858 2096907 50 5 [3] [3] 4 [wbbL]–[wbbK] [wbbL],[wbbK]
* * ÷ NZ_CP009273 2768272 2768341 70 6 [3] [3] 5 BW25113_RS13770 hypothetical protein
* * ÷ NZ_CP009273 2975709 2975779 71 4 [2] [3] 5 araE/kduD arabinose‑proton symporter AraE/2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase KduD
* * ÷ NZ_CP009273 2988540 2988671 132 4 [2] [3] 5 BW25113_RS14850/BW25113_RS26010 response regulator transcription factor/hypothetical protein
* * ÷ NZ_CP009273 3629488 3631696 2209 43 [0] [2] 4 yhiN–[pitA] yhiN,[pitA]
* * ÷ NZ_CP009273 3631732 3633354 1623 4 [3] [3] 4 [pitA]–uspB [pitA],uspB
* * ÷ NZ_CP009273 3633361 3634006 646 4 [3] [3] 4 uspA–[BW25113_RS26230] uspA,[BW25113_RS26225],[BW25113_RS26230]
* * ÷ NZ_CP009273 3634012 3638127 4116 4 [3] [2] 4 [BW25113_RS26225]–[prlC] [BW25113_RS26225],[BW25113_RS26230],dtpB,rsmJ,[prlC]
* * ÷ NZ_CP009273 3638188 3642299 4112 4 [2] [3] 4 [prlC]–[arsB] [prlC],rlmJ,gorA,dinQ,arsR,[arsB]
* * ÷ NZ_CP009273 3642385 3646524–3645398 3014–4140 4 [2] [3] 4 [arsB]–BW25113_RS18160 [arsB],arsC,BW25113_RS18155,BW25113_RS18160
* * ÷ NZ_CP009273 4250367 4250399 33 4 [3] [3] 5 BW25113_RS21020 DUF2713 domain‑containing protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)43 (1.280) 22/164 0.3 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =2 (0.060)24 (0.890) 18/132 0.3 96.8% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 0 (0.000)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032612 (0.060)18 (0.670) 16/132 0.4 95.7% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 0 (0.000)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)19 (0.570) 13/162 0.8 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)37 (1.100) 22/164 0.3 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 2282398 =NA (NA)42 (1.250) 20/164 0.4 100% pseudogene (3/2532 nt) yejO autotransporter YejO
?NZ_CP009273 = 3629487 0 (0.000)intergenic (+234/+81) yhiM/yhiN DUF2776 domain‑containing protein/NAD(P)/FAD‑dependent oxidoreductase
* ? NZ_CP009273 4532499 =0 (0.000)9 (0.300) 6/146 1.6 90.0% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 2 (0.070)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325070 (0.000)14 (0.470) 10/146 1.0 93.3% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 2 (0.070)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA