breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,884 C→A P21Q (CCG→CAG)  folA → type 3 dihydrofolate reductase
MC JC 1,613,631 Δ3 bp coding (255‑257/435 nt) marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,632,461 T→G L487R (CTT→CGT)  pitA → inorganic phosphate transporter PitA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 153689 153723 35 4 [1] [0] 2 yadN/folK fimbrial protein/2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
* * ÷ NZ_CP009273 356981 357570 590 5 [0] [1] 2 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 647494 647554 61 2 [1] [1] 2 citC/dpiB [citrate (pro‑3S)‑lyase] ligase/sensor histidine kinase DpiB
* * ÷ NZ_CP009273 729529 729534 6 2 [0] [1] 2 BW25113_RS03630 hypothetical protein
* * ÷ NZ_CP009273 869145 869174 30 2 [1] [1] 3 pdeI EAL domain‑containing protein
* * ÷ NZ_CP009273 1098970 1099001 32 2 [0] [1] 2 csgD/csgB biofilm master transcriptional regulator CsgD/curli minor subunit CsgB
* * ÷ NZ_CP009273 1207491 1207538 48 2 [1] [0] 2 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1207612 1207655 44 2 [0] [1] 3 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1210439 1210483 45 2 [1] [1] 2 bluF blue light‑responsive regulator BluF
* * ÷ NZ_CP009273 1225875 1225927 53 2 [0] [1] 3 hlyE/umuD hemolysin HlyE/translesion error‑prone DNA polymerase V autoproteolytic subunit
* * ÷ NZ_CP009273 1314765 1314802 38 3 [1] [1] 2 trpD bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
* * ÷ NZ_CP009273 1363740 1363764 25 2 [1] [0] 2 pspD phage shock protein PspD
* * ÷ NZ_CP009273 1459374 1459483 110 4 [1] [1] 3 [BW25113_RS25580] [BW25113_RS25580]
* * ÷ NZ_CP009273 1524460 1524472 13 2 [1] [0] 2 BW25113_RS26145 hypothetical protein
* * ÷ NZ_CP009273 1631964 1631993 30 2 [0] [0] 4 gnsB addiction module toxin, GnsA/GnsB family
* * ÷ NZ_CP009273 1836615 1836615 1 2 [0] [0] 2 ynjH/gdhA YnjH family protein/NADP‑specific glutamate dehydrogenase
* * ÷ NZ_CP009273 1864668 1864731 64 4 [1] [1] 5 cdgI GGDEF domain‑containing protein
* * ÷ NZ_CP009273 1888062 1888087 26 2 [1] [0] 2 yoaB/yoaC RidA family protein/DUF1889 family protein
* * ÷ NZ_CP009273 2004892 2004900 9 2 [1] [1] 2 BW25113_RS10120 DUF5503 domain‑containing protein
* * ÷ NZ_CP009273 2449501 2449508 8 2 [0] [0] 2 yfcV/sixA fimbrial protein/phosphohistidine phosphatase SixA
* * ÷ NZ_CP009273 2462903 2462919 17 2 [1] [0] 2 BW25113_RS12270 hypothetical protein
* * ÷ NZ_CP009273 2897143 2897193 51 2 [1] [1] 2 ygcE/queE FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
* * ÷ NZ_CP009273 2988543 2988563 21 2 [1] [0] 2 BW25113_RS14850/BW25113_RS26010 response regulator transcription factor/hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)44 (1.860) 26/164 0.1 50.6% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 43 (1.820)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =16 (0.680)3 (0.160) 3/132 1.6 23.2% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 7 (0.370)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)10 (0.430) 8/162 0.9 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)43 (1.820) 23/164 0.1 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 4532499 =3 (0.130)13 (0.620) 9/146 0.7 77.2% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 5 (0.240)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325073 (0.130)9 (0.430) 7/146 0.9 70.1% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 5 (0.240)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA